Phosphophoryn (PP) and dentin sialoprotein (DSP) are the most dominant non-collagenous proteins in dentin. PP is an extremely acidic protein that can function as a mineral nucleator for dentin mineralization. DSP was ...Phosphophoryn (PP) and dentin sialoprotein (DSP) are the most dominant non-collagenous proteins in dentin. PP is an extremely acidic protein that can function as a mineral nucleator for dentin mineralization. DSP was first identified in 1981, yet its functional significance is still controversial. Historically, these two proteins were considered to be independently synthesized and secreted by dental pulp cells into the developing dentin matrix. However, with the identification of the DSP coding sequence in 1994, followed2 years later by the finding that the PP coding sequence was located immediately downstream from the DSP sequence, it became immediately clear that DSP and PP proteins were derived from a single DSP-PP (i.e., dentin sialophosphoprotein, DSPP) transcript.Since DSPP cDNA became available, tremendous progress has been made in studying DSP-PP mRNA distribution and DSP generation from the DSP-PP precursor protein at specific cleavage sites by protease tolloid-related-1 (TLR1) or bone morphogenetic protein 1 (BMP1). The functions of DSP-PP and DSP were investigated via DSP-PP knockout (KO) and DSP knockin in DSP-PP KO mice. In addition, a number of in vitro studies aimed to elucidate DSPP and DSP function in dental pulp cells.展开更多
Natural genetic polymorphisms are gifts from nature and sources of variations at all levels.The postgenomic era permits new perspectives on interpreting genetic polymorphisms and also poses challenges for scientists t...Natural genetic polymorphisms are gifts from nature and sources of variations at all levels.The postgenomic era permits new perspectives on interpreting genetic polymorphisms and also poses challenges for scientists to answer system questions.Rather than a comprehensive coverage of genetic polymorphisms upto-date,this review attempts to present some of the results in a somewhat coherent manner to highlight the needs and potentials of pertinent studies.展开更多
Tests of preoperative visual function and prediction of postoperative E chart visual acuity(ECVA) using laser interferometric visual acuity(LIVA) and electroretinogram(ERG) were performed in 16 cases(19 eyes) of catar...Tests of preoperative visual function and prediction of postoperative E chart visual acuity(ECVA) using laser interferometric visual acuity(LIVA) and electroretinogram(ERG) were performed in 16 cases(19 eyes) of cataract. The results showed that the coincident rate between preoperative LIVA and postoperative ECVA was 63.2%, and there was a parallel correlation between preoperative amplitude of photopic ERG b-wave and postoperative ECVA in 79.0% of the eyes. Comparing these two methods, the test of LIVA ...展开更多
Coronary lesions of intermediate severity, defined as 50%-70% luminal diameter narrowing by visualestimation at angiography, are frequently encountered in patients with stable or unstable coronary artery disease. Clin...Coronary lesions of intermediate severity, defined as 50%-70% luminal diameter narrowing by visualestimation at angiography, are frequently encountered in patients with stable or unstable coronary artery disease. Clinical decision making for patients with intermediate coronary stenosis is still challenging, but may be facilitated by assessing the morphology and physiological significance of these lesions.1 It is clear that angiographic stenosis is a highly unreliable surrogate for myocardial ischemia, as a significant proportion of anatomically high-grade lesions do not cause ischemia. Conversely, even for anatomically mild coronary lesions, a non-negligible rate of myocardial ischemia is consistently noted. Because the physiological significance of a lesion is mainly determined by both the severity of a stenosis and the amount of myocardium supplied, coronary angiography alone does not accurately predict the functional significance of intermediate coronary lesions.2,3展开更多
To improve the efficiency of safety tests of driver-automation cooperation,a method for generating a scenario library is proposed that considers the probability of scenario occurrence and driver-handling challenges in...To improve the efficiency of safety tests of driver-automation cooperation,a method for generating a scenario library is proposed that considers the probability of scenario occurrence and driver-handling challenges in real driving situations.First,the original scenario data under cut-in conditions stored in a time series are extracted from the scenario data set.Then,a mathematical performance index is used to model the scenario and a significance function in terms of the occurrence frequency of the scenario,and the performance challenge between the driver and the vehicle is established.Next,the important scenario set is extracted from the original scenario set by constructing and optimizing a significance auxiliary function.Finally,the extracted important scenario sets are filtered by using the significance function values of the scenarios to generate a scenario library.Simulation results show that the proposed method for scenario library generation can effectively identify scenarios with potential adventure during driver-automation cooperation and thus accelerate safety tests compared with traditional methods.展开更多
Activating transcription factor 2(ATF2)is a member of the ATF/cyclic AMP-responsive element bind-ing protein family of transcription factors.However,the information concerning ATF2 ion-mediated DNA binding module and ...Activating transcription factor 2(ATF2)is a member of the ATF/cyclic AMP-responsive element bind-ing protein family of transcription factors.However,the information concerning ATF2 ion-mediated DNA binding module and function of ATF2 in malignant pleural mesothelioma(MPM)has never been addressed.In this study,by using GRNInfer and GVedit based on linear pro-gramming and a decomposition procedure,with integrated analysis of the function cluster using Kappa statistics and fuzzy heuristic clustering in MPM,we identified one ATF2 ion-mediated DNA binding module involved in invasive function including ATF2 inhibition to target genes FALZ,C20orf31,NME2,PLOD2,RNF10,and RNASEH1,upstream RNF10 and PLOD2 activation to ATF2,upstream RNASEH1 and FALZ inhibition to ATF2 from 40 MPM tumors and 5 normal pleural tissues.Remarkably,our results showed that the predominant effect of ATF2 occupancy is to suppress the activation of target genes on MPM.Importantly,the ATF2 ion-mediated DNA binding module reflects‘mutual’positive and negative feedback regulation mechanism of ATF2 with up-and down-stream genes.It may be useful for developing novel prognostic markers and therapeutic targets in MPM.展开更多
文摘Phosphophoryn (PP) and dentin sialoprotein (DSP) are the most dominant non-collagenous proteins in dentin. PP is an extremely acidic protein that can function as a mineral nucleator for dentin mineralization. DSP was first identified in 1981, yet its functional significance is still controversial. Historically, these two proteins were considered to be independently synthesized and secreted by dental pulp cells into the developing dentin matrix. However, with the identification of the DSP coding sequence in 1994, followed2 years later by the finding that the PP coding sequence was located immediately downstream from the DSP sequence, it became immediately clear that DSP and PP proteins were derived from a single DSP-PP (i.e., dentin sialophosphoprotein, DSPP) transcript.Since DSPP cDNA became available, tremendous progress has been made in studying DSP-PP mRNA distribution and DSP generation from the DSP-PP precursor protein at specific cleavage sites by protease tolloid-related-1 (TLR1) or bone morphogenetic protein 1 (BMP1). The functions of DSP-PP and DSP were investigated via DSP-PP knockout (KO) and DSP knockin in DSP-PP KO mice. In addition, a number of in vitro studies aimed to elucidate DSPP and DSP function in dental pulp cells.
基金supported by grants from the Chinese Academy of Sciences(No.171846).
文摘Natural genetic polymorphisms are gifts from nature and sources of variations at all levels.The postgenomic era permits new perspectives on interpreting genetic polymorphisms and also poses challenges for scientists to answer system questions.Rather than a comprehensive coverage of genetic polymorphisms upto-date,this review attempts to present some of the results in a somewhat coherent manner to highlight the needs and potentials of pertinent studies.
文摘Tests of preoperative visual function and prediction of postoperative E chart visual acuity(ECVA) using laser interferometric visual acuity(LIVA) and electroretinogram(ERG) were performed in 16 cases(19 eyes) of cataract. The results showed that the coincident rate between preoperative LIVA and postoperative ECVA was 63.2%, and there was a parallel correlation between preoperative amplitude of photopic ERG b-wave and postoperative ECVA in 79.0% of the eyes. Comparing these two methods, the test of LIVA ...
文摘Coronary lesions of intermediate severity, defined as 50%-70% luminal diameter narrowing by visualestimation at angiography, are frequently encountered in patients with stable or unstable coronary artery disease. Clinical decision making for patients with intermediate coronary stenosis is still challenging, but may be facilitated by assessing the morphology and physiological significance of these lesions.1 It is clear that angiographic stenosis is a highly unreliable surrogate for myocardial ischemia, as a significant proportion of anatomically high-grade lesions do not cause ischemia. Conversely, even for anatomically mild coronary lesions, a non-negligible rate of myocardial ischemia is consistently noted. Because the physiological significance of a lesion is mainly determined by both the severity of a stenosis and the amount of myocardium supplied, coronary angiography alone does not accurately predict the functional significance of intermediate coronary lesions.2,3
基金Major Project of Scientific and Technological Innovation 2030“New Generation Artificial Intelligence”(Grant No.2020AAA0108105)National Nature Science Foundation of China(Grants Nos.62103162 and U19A2069)+1 种基金Jilin Key Research and Development Program(Grant No.20200401088GX)the Jilin Major Science and Technology Projects(Grant No.20200501011GX).
文摘To improve the efficiency of safety tests of driver-automation cooperation,a method for generating a scenario library is proposed that considers the probability of scenario occurrence and driver-handling challenges in real driving situations.First,the original scenario data under cut-in conditions stored in a time series are extracted from the scenario data set.Then,a mathematical performance index is used to model the scenario and a significance function in terms of the occurrence frequency of the scenario,and the performance challenge between the driver and the vehicle is established.Next,the important scenario set is extracted from the original scenario set by constructing and optimizing a significance auxiliary function.Finally,the extracted important scenario sets are filtered by using the significance function values of the scenarios to generate a scenario library.Simulation results show that the proposed method for scenario library generation can effectively identify scenarios with potential adventure during driver-automation cooperation and thus accelerate safety tests compared with traditional methods.
基金supported by the National Natural Science Foundation of China(Grant No.60673109)the Teaching and Scientific Research Foundation for the Returned Overseas Chinese Scholars,Ministry of Education of China.
文摘Activating transcription factor 2(ATF2)is a member of the ATF/cyclic AMP-responsive element bind-ing protein family of transcription factors.However,the information concerning ATF2 ion-mediated DNA binding module and function of ATF2 in malignant pleural mesothelioma(MPM)has never been addressed.In this study,by using GRNInfer and GVedit based on linear pro-gramming and a decomposition procedure,with integrated analysis of the function cluster using Kappa statistics and fuzzy heuristic clustering in MPM,we identified one ATF2 ion-mediated DNA binding module involved in invasive function including ATF2 inhibition to target genes FALZ,C20orf31,NME2,PLOD2,RNF10,and RNASEH1,upstream RNF10 and PLOD2 activation to ATF2,upstream RNASEH1 and FALZ inhibition to ATF2 from 40 MPM tumors and 5 normal pleural tissues.Remarkably,our results showed that the predominant effect of ATF2 occupancy is to suppress the activation of target genes on MPM.Importantly,the ATF2 ion-mediated DNA binding module reflects‘mutual’positive and negative feedback regulation mechanism of ATF2 with up-and down-stream genes.It may be useful for developing novel prognostic markers and therapeutic targets in MPM.
