[Objective] The aim was to explore the genetic differences among F2 progenies of sugarcane.[Method] 11 primers were used for ISSR analysis on 20 F2 hybrids of Erianthus fulvus.[Result] A total of 121 bands were amplif...[Objective] The aim was to explore the genetic differences among F2 progenies of sugarcane.[Method] 11 primers were used for ISSR analysis on 20 F2 hybrids of Erianthus fulvus.[Result] A total of 121 bands were amplified in the PCR reactions,among which 110 were polymorphic bands and the polymorphic rate was 90.91%,indicating that there were abundance genetic variation among F2 progenies derived from cross combinations between cultivated sugarcane and E.fulvus.The average genetic coefficients between Yacheng 89/9 and F1 progenies,E.fulvus and F1 progenies,were 0.71 and 0.54,respectively.The clustering result of F2 progenies showed that all the progenies clustered with female parent and suggested that the genetic material of female parent was predominated in F2 progenies.[Conclusion] The study could provide basis for the application of sugarcane innovation germplasm materials in the breeding of sugarcane.展开更多
Natal dispersal is a critical trait for individual ftness and the viability,structure,and genetic identity of populations.However,there is a pronounced information gap for large and long-lived species due to the diffc...Natal dispersal is a critical trait for individual ftness and the viability,structure,and genetic identity of populations.However,there is a pronounced information gap for large and long-lived species due to the diffculty of monitoring individuals at appropriate spatio-temporal scales.Here we study how individual traits and social and environmental characteristics infuence natal dispersal decisions of griffon vultures(Gypsfulvus)using long-term(30 years)monitoring of a large number of individuals marked as nestlings in Spain.Our results show a strong philopatryin both sexes,with some individuals recruiting as breeders on the same cliffs,and even the same nests,where they were born.This philopatrictendency was modulated by the effect of conspecifc density on individual parameters and emphasized the importance of conspecifc attraction and changes in food availability that may have infuenced the increment in colony size and the colonization of new areas.Although furtherresearch is needed considering smaller colonies and more isolated population nuclei,our results highlight the importance of long-term studieson long-lived species to understand the factors that determine their population dynamics and their relationship with anthropogenic activities,whose effects should be predicted and managed using conservation criteria.展开更多
In this study, twelve primers were used for ISSR （ inter simple sequence repeat） analysis of eight F1 hybrids of Erianthu.sfulvus and sugarcane （ Saccha- rum spp. ）. The results showed that totally 133 bands were ...In this study, twelve primers were used for ISSR （ inter simple sequence repeat） analysis of eight F1 hybrids of Erianthu.sfulvus and sugarcane （ Saccha- rum spp. ）. The results showed that totally 133 bands were amplified, including 111 polymorphic bands, showing a polymorphic rate of 83.46%, which indicated that there are abundant genetic variations among F1 progenies derived from crossing between cultivated sugarcane and E. fu/vus. The average genetic similarity coef- ficients between F1 progenies and female parent Yacheng 89-9, F1 progenies and male parent E. fidvas were 0.67 and 0.44, respectively. UPGMA clustering result of F1 progenies showed that all the progenies were clustered with female parent, suggesting that the genetic material of female parent was predominated in F： proge- nies, which also revealed that the chromosome number of F, progenies was not directly related to the clustering results based on ISSR analysis.展开更多
In this study, karyotype analysis of F2BCl progenies between sugarcane （Saccharum spp. ） and Erianthusfulvus was conducted. The result showed that most chromosomes of YAU04/14, YAU09/02, YAU09/05, YAU09/26 and YAU09...In this study, karyotype analysis of F2BCl progenies between sugarcane （Saccharum spp. ） and Erianthusfulvus was conducted. The result showed that most chromosomes of YAU04/14, YAU09/02, YAU09/05, YAU09/26 and YAU09/52 are median region （m） chromosomes, while only a small amount of chromosomes are submedian （sin） chromosomes and median point （M） chromosomes. The karyotype formulae of five experimental materials are 2n = 106 = 98m ＋ 8sm, 2n = 106 - 2M ＋ 104m, 2n = 106 = 4M ＋ 96m ＋ 6sin, 2n = 102 = 2M ＋ 92m ＋ 8sin and 2n = 106 = 4M ＋ 96m ＋ 6sin, respectively. The karyotypes of all materials belong to symmetrical 2B type, indicating that the chromosomes of progenies are primitive.展开更多
Variation in offspring sex ratios is a central topic in animal demography and population dynamics.Most studies have focused on bird species with marked sexual dimorphism and multiple-nestling broods,where the offsprin...Variation in offspring sex ratios is a central topic in animal demography and population dynamics.Most studies have focused on bird species with marked sexual dimorphism and multiple-nestling broods,where the offspring sex ratio is often biased due to different individual or environmental variables.However,biases in offspring sex ratios have been far less investigated in monomorphic and single-egg laying species,and few studies have evaluated long-term and large-scale variations in the sex ratio of nestling vultures.Here,we explore individual and environmental factors potentially affecting the secondary sex ratio of the monomorphic griffon vulture Gyps fulvus.We used information collected at three breeding nuclei from central Spain over a 30-year period(1990–2020)to analyse the effects of nestling age,parental age,breeding phenology,conspecific density,population reproductive parameters,and spatial and temporal variability on nestling sex.Sex ratio did not differ from parity either at the population or the nuclei level.No significant between-year differences were detected,even under highly changing conditions of food availability associated with the mad-cow crisis.We found that tree nesting breeders tend to have more sons than daughters,but as this nesting behavior is rare and we consequently have a small sample size,this issue would require additional examination.Whereas further research is needed to assess the potential effect of breeder identity on nestling sex ratio,this study contributes to understanding the basic ecology and population dynamics of Griffon Vultures,a long-lived species with deferred maturity and low fecundity,whose minor deviations in the offspring sex ratio might imply major changes at the population level.展开更多
The present work documented two snapper species, Lutjanus fulvus Forster, 1801 and Lutjanus erythropterus Bloch, 1790, recently collected from the Saint Martin's Island, Bay of Bengal, Bangladesh, during a survey ...The present work documented two snapper species, Lutjanus fulvus Forster, 1801 and Lutjanus erythropterus Bloch, 1790, recently collected from the Saint Martin's Island, Bay of Bengal, Bangladesh, during a survey on coral associated fishes of Bangladesh from July 2017 to September 2018. These two species were identified with morphological characteristics and by analyzing partial mitochondrial COI sequences. The document also included an updated checklist of snappers available in Bangladesh.展开更多
During the last years many authors have char-acterized the produced formation waters (PFWs) with respect to chemical compounds and toxic-ity. Most of data are related to PFWs collected on offshore platform after treat...During the last years many authors have char-acterized the produced formation waters (PFWs) with respect to chemical compounds and toxic-ity. Most of data are related to PFWs collected on offshore platform after treatment process. The available results showed that the particulate phase had an influence on PFW toxicity. As-suming the toxicity of PFWs treated on platform, the aim of this paper is to study the toxicity of these PFWs after a further filtration treatment carried out in laboratory. For this purpose PFWs were sampled from three natural gas platforms located in the Adriatic Sea (Italy) below treat-ment system. The eco-toxicological bioassays have been conducted on test-organisms be-longing to different trophic levels such as bac-teria, algae, crustaceans and fishes. The PFWs resulted toxic according to an overall assess-ment obtained through the bioassays. Further-more, it has been possible to identify the spe-cies that were more sensitive to the tested PFWs, namely Tigriopus fulvus, Dicentrarchus labrax and Vibrio fischeri. Besides, a chemical char-acterization was reported related to the con-taminants present in the PFWs to go with eco-toxicological assessment. Barium, zinc and manganese showed the most concentrations among the metals and the lower molecular weight components were common among the organic compounds. Some differences among PFWs were observed both for toxicity and chemical composition. The highest toxicity was recorded in PFWs (PFW1 and PFW2) containing the highest concentrations of some metals (Ba, Mn and Zn) and/or BTEX.展开更多
Erianthus produces substantial biomass,exhibits a good Brix value,and shows wide environmental adaptability,making it a potential biofuel plant.In contrast to closely related sorghum and sugarcane,Erianthus can grow i...Erianthus produces substantial biomass,exhibits a good Brix value,and shows wide environmental adaptability,making it a potential biofuel plant.In contrast to closely related sorghum and sugarcane,Erianthus can grow in degraded soils,thus releasing pressure on agricultural lands used for biofuel production.However,the lack of genomic resources for Erianthus hinders its genetic improvement,thus limiting its potential for biofuel production.In the present study,we generated a chromosome-scale reference genome for Erianthus fulvus Nees.The genome size estimated by flow cytometry was 937 Mb,and the assembled genome size was 902 Mb,covering 96.26%of the estimated genome size.A total of 35065 proteincoding genes were predicted,and 67.89%of the genome was found to be repetitive.A recent wholegenome duplication occurred approximately 74.10 million years ago in the E.fulvus genome.Phylogenetic analysis showed that E.fulvus is evolutionarily closer to S.spontaneum and diverged after S.bicolor.Three of the 10 chromosomes of E.fulvus formed through rearrangements of ancestral chromosomes.Phylogenetic reconstruction of the Saccharum complex revealed a polyphyletic origin of the complex and a sister relationship of E.fulvus with Saccharum sp.,excluding S.arundinaceum.On the basis of the four amino acid residues that provide substrate specificity,the E.fulvus SWEET proteins were classified as monoand disaccharide sugar transporters.Ortho-QTL genes identified for 10 biofuel-related traits may aid in the rapid screening of E.fulvus populations to enhance breeding programs for improved biofuel production.The results of this study provide valuable insights for breeding programs aimed at improving biofuel production in E.fulvus and enhancing sugarcane introgression programs.展开更多
基金Supported by National Natural Science Foundation Project of China(30960045)Key and New Products Launch Program of Yunnan Province(2010BB011)+1 种基金Talent Training Program of Yunnan Province(2006PY01-38)Sugarcane Industry Technical System Construction Project of Yunnan Province(2009-2013)~~
文摘[Objective] The aim was to explore the genetic differences among F2 progenies of sugarcane.[Method] 11 primers were used for ISSR analysis on 20 F2 hybrids of Erianthus fulvus.[Result] A total of 121 bands were amplified in the PCR reactions,among which 110 were polymorphic bands and the polymorphic rate was 90.91%,indicating that there were abundance genetic variation among F2 progenies derived from cross combinations between cultivated sugarcane and E.fulvus.The average genetic coefficients between Yacheng 89/9 and F1 progenies,E.fulvus and F1 progenies,were 0.71 and 0.54,respectively.The clustering result of F2 progenies showed that all the progenies clustered with female parent and suggested that the genetic material of female parent was predominated in F2 progenies.[Conclusion] The study could provide basis for the application of sugarcane innovation germplasm materials in the breeding of sugarcane.
基金partially funded through projects PID2019-109685GB-I00,CGL2007-61395,CGL2010-15726,and CGL2013-42451-P,of the Spanish Ministry of Science and Innovation.
文摘Natal dispersal is a critical trait for individual ftness and the viability,structure,and genetic identity of populations.However,there is a pronounced information gap for large and long-lived species due to the diffculty of monitoring individuals at appropriate spatio-temporal scales.Here we study how individual traits and social and environmental characteristics infuence natal dispersal decisions of griffon vultures(Gypsfulvus)using long-term(30 years)monitoring of a large number of individuals marked as nestlings in Spain.Our results show a strong philopatryin both sexes,with some individuals recruiting as breeders on the same cliffs,and even the same nests,where they were born.This philopatrictendency was modulated by the effect of conspecifc density on individual parameters and emphasized the importance of conspecifc attraction and changes in food availability that may have infuenced the increment in colony size and the colonization of new areas.Although furtherresearch is needed considering smaller colonies and more isolated population nuclei,our results highlight the importance of long-term studieson long-lived species to understand the factors that determine their population dynamics and their relationship with anthropogenic activities,whose effects should be predicted and managed using conservation criteria.
基金Supported by Key New Product Development Project of Yunnan Province(2010BB011)National Natural Science Foundation of China (30960045)+2 种基金Yunnan Agricultural Science and Technology Key Project during the Eleventh Five-Year Plan (2006NG11)Talent Training Project of Yunnan Province(2006PY-38)Yunnan Provincial Modern Agricultural Industry Technology System Construction Project of Sugarcane Breeding Research Center
文摘In this study, twelve primers were used for ISSR （ inter simple sequence repeat） analysis of eight F1 hybrids of Erianthu.sfulvus and sugarcane （ Saccha- rum spp. ）. The results showed that totally 133 bands were amplified, including 111 polymorphic bands, showing a polymorphic rate of 83.46%, which indicated that there are abundant genetic variations among F1 progenies derived from crossing between cultivated sugarcane and E. fu/vus. The average genetic similarity coef- ficients between F1 progenies and female parent Yacheng 89-9, F1 progenies and male parent E. fidvas were 0.67 and 0.44, respectively. UPGMA clustering result of F1 progenies showed that all the progenies were clustered with female parent, suggesting that the genetic material of female parent was predominated in F： proge- nies, which also revealed that the chromosome number of F, progenies was not directly related to the clustering results based on ISSR analysis.
基金Supported by National Natural Science Foundation of China(30960045)Program for Construction of Modern Agricultural Sugarcane Industry Technology System of Yunnan Province(YCJ[2014]No.105)+3 种基金Key Project of New Product Development of Yunnan Province(Agriculture,2012BB014)Innovation Team Project for Sustainable Production System of Mountain Plateau Crops of Yunnan Agricultural University(YKRF[2012]No.18)Research Fund for the Doctoral Program of Yunnan Agricultural University(A2002181)General Program of Applied Basic Research of Yunnan Province(2013FB037)
文摘In this study, karyotype analysis of F2BCl progenies between sugarcane （Saccharum spp. ） and Erianthusfulvus was conducted. The result showed that most chromosomes of YAU04/14, YAU09/02, YAU09/05, YAU09/26 and YAU09/52 are median region （m） chromosomes, while only a small amount of chromosomes are submedian （sin） chromosomes and median point （M） chromosomes. The karyotype formulae of five experimental materials are 2n = 106 = 98m ＋ 8sm, 2n = 106 - 2M ＋ 104m, 2n = 106 = 4M ＋ 96m ＋ 6sin, 2n = 102 = 2M ＋ 92m ＋ 8sin and 2n = 106 = 4M ＋ 96m ＋ 6sin, respectively. The karyotypes of all materials belong to symmetrical 2B type, indicating that the chromosomes of progenies are primitive.
基金The study was carried out with the permission of the regional government of Castilla y Leon,Direccion General del Medio Natural,Servicio de Espacios Naturales.Research was funded by the Spanish Ministry of Economy,Industry,and Competitiveness through projects CGL2007-61395,CGL2010-15726,CGL2013-42451-PPID2019-109685GB-I00 of the Spanish Ministry of Science and Innovation+1 种基金G.G.L.was supported by a FPU fellowship of the Spanish Ministry of Science,Innovation and Universities(FPU19/06511)A.S.A.was supported by a Ramon y Cajal fellowship of the Spanish Ministry of Science(RYC-2017-22796).
文摘Variation in offspring sex ratios is a central topic in animal demography and population dynamics.Most studies have focused on bird species with marked sexual dimorphism and multiple-nestling broods,where the offspring sex ratio is often biased due to different individual or environmental variables.However,biases in offspring sex ratios have been far less investigated in monomorphic and single-egg laying species,and few studies have evaluated long-term and large-scale variations in the sex ratio of nestling vultures.Here,we explore individual and environmental factors potentially affecting the secondary sex ratio of the monomorphic griffon vulture Gyps fulvus.We used information collected at three breeding nuclei from central Spain over a 30-year period(1990–2020)to analyse the effects of nestling age,parental age,breeding phenology,conspecific density,population reproductive parameters,and spatial and temporal variability on nestling sex.Sex ratio did not differ from parity either at the population or the nuclei level.No significant between-year differences were detected,even under highly changing conditions of food availability associated with the mad-cow crisis.We found that tree nesting breeders tend to have more sons than daughters,but as this nesting behavior is rare and we consequently have a small sample size,this issue would require additional examination.Whereas further research is needed to assess the potential effect of breeder identity on nestling sex ratio,this study contributes to understanding the basic ecology and population dynamics of Griffon Vultures,a long-lived species with deferred maturity and low fecundity,whose minor deviations in the offspring sex ratio might imply major changes at the population level.
基金funded by a competitive research grant of PIU-BARC, NATP-2 project of Bangladesh Agricultural Research Council。
文摘The present work documented two snapper species, Lutjanus fulvus Forster, 1801 and Lutjanus erythropterus Bloch, 1790, recently collected from the Saint Martin's Island, Bay of Bengal, Bangladesh, during a survey on coral associated fishes of Bangladesh from July 2017 to September 2018. These two species were identified with morphological characteristics and by analyzing partial mitochondrial COI sequences. The document also included an updated checklist of snappers available in Bangladesh.
文摘During the last years many authors have char-acterized the produced formation waters (PFWs) with respect to chemical compounds and toxic-ity. Most of data are related to PFWs collected on offshore platform after treatment process. The available results showed that the particulate phase had an influence on PFW toxicity. As-suming the toxicity of PFWs treated on platform, the aim of this paper is to study the toxicity of these PFWs after a further filtration treatment carried out in laboratory. For this purpose PFWs were sampled from three natural gas platforms located in the Adriatic Sea (Italy) below treat-ment system. The eco-toxicological bioassays have been conducted on test-organisms be-longing to different trophic levels such as bac-teria, algae, crustaceans and fishes. The PFWs resulted toxic according to an overall assess-ment obtained through the bioassays. Further-more, it has been possible to identify the spe-cies that were more sensitive to the tested PFWs, namely Tigriopus fulvus, Dicentrarchus labrax and Vibrio fischeri. Besides, a chemical char-acterization was reported related to the con-taminants present in the PFWs to go with eco-toxicological assessment. Barium, zinc and manganese showed the most concentrations among the metals and the lower molecular weight components were common among the organic compounds. Some differences among PFWs were observed both for toxicity and chemical composition. The highest toxicity was recorded in PFWs (PFW1 and PFW2) containing the highest concentrations of some metals (Ba, Mn and Zn) and/or BTEX.
基金supported by grants from the Major Science and Technology Projects in Yunnan Province(202202AE090021)a special project of Yunnan Key Laboratory of Crop Production and Smart Agriculture(202105AG070007)+3 种基金a sub-project of the National Key Research and Development Program of China(2018YFD1000503)the National Natural Science Foundation of China(31960451,31560417)a Key Project of Applied Basic Research Program of Yunnan Province(2015FA024)the ESI Discipline Promotion Program of Yunnan Agricultural University(2019YNAUESIMS01).
文摘Erianthus produces substantial biomass,exhibits a good Brix value,and shows wide environmental adaptability,making it a potential biofuel plant.In contrast to closely related sorghum and sugarcane,Erianthus can grow in degraded soils,thus releasing pressure on agricultural lands used for biofuel production.However,the lack of genomic resources for Erianthus hinders its genetic improvement,thus limiting its potential for biofuel production.In the present study,we generated a chromosome-scale reference genome for Erianthus fulvus Nees.The genome size estimated by flow cytometry was 937 Mb,and the assembled genome size was 902 Mb,covering 96.26%of the estimated genome size.A total of 35065 proteincoding genes were predicted,and 67.89%of the genome was found to be repetitive.A recent wholegenome duplication occurred approximately 74.10 million years ago in the E.fulvus genome.Phylogenetic analysis showed that E.fulvus is evolutionarily closer to S.spontaneum and diverged after S.bicolor.Three of the 10 chromosomes of E.fulvus formed through rearrangements of ancestral chromosomes.Phylogenetic reconstruction of the Saccharum complex revealed a polyphyletic origin of the complex and a sister relationship of E.fulvus with Saccharum sp.,excluding S.arundinaceum.On the basis of the four amino acid residues that provide substrate specificity,the E.fulvus SWEET proteins were classified as monoand disaccharide sugar transporters.Ortho-QTL genes identified for 10 biofuel-related traits may aid in the rapid screening of E.fulvus populations to enhance breeding programs for improved biofuel production.The results of this study provide valuable insights for breeding programs aimed at improving biofuel production in E.fulvus and enhancing sugarcane introgression programs.
