The ovary is an excellent system for studying stem cell renewal and differentiation, which is under the control of ovarian somatic cells. In order to understand oogenesis in Fugu rubripes （Temminck et. Schlegel） as ...The ovary is an excellent system for studying stem cell renewal and differentiation, which is under the control of ovarian somatic cells. In order to understand oogenesis in Fugu rubripes （Temminck et. Schlegel） as a marine fish model of aquaculture importance, we established cell lines called TSOC 1 and TSOC2 from a juvenile ovary of this organism. TSOC1 is composed of spindle epithelial-like cells, while the other is cobblestone-like ceils. Therefore, TSOC1 and TSOC2 appear to consist of ovarian somatic cells. Growth requirement condition was investigated including temperature, concentration of FBS and pH. Significant fluorescent signals were observed after TSOC1 and TSOC2 cells were transfected with pEGFP-N3 vector, indicating its potential utility for genetic manipulation such as gene function studies. It is shown that these cell lines are effective for infection by the turbot reddish body iridovirus and flounder lymphosystis disease virus as evidenced by the appearance of cytopathic effect and virus propagation in the virus-infected cells, and most convincingly, the observation of viral particles by electron microscopy, demonstrating that TSOC1 and TSOC2 are suitable to study interactions between virus and host cells. It is believed that TSOC1 and TSOC2 will be useful tools to study sex-related events and interactions between primordial germ cells and oogonia cells during oogenesis. Therefore, establishment of ovary cell lines from Fugu rubripes seems to be significant for those research areas.展开更多
Based on the Leaderman constitutive relations in nonlinear viscoelasticity and the linear geometrical assumption, a mathematical model for the bending of nonlinear viscoelastic beams was established in this paper. The...Based on the Leaderman constitutive relations in nonlinear viscoelasticity and the linear geometrical assumption, a mathematical model for the bending of nonlinear viscoelastic beams was established in this paper. The Laplace transformation method and the Titchmarsh theorem were used to prove that some relations exist between solutions to bending problems of visco- and elastic beams, which reveals the fugue effect of viscoelastic materials. The high-order Galerkin approximate solution to the quasi-static response of nonlinear viscoelastic beams under a step load was obtained by using the new method suggested in this paper as well as the Mathematica software and the Newton iteration technique.展开更多
In this study, genes of two distinct tissue inhibitors of metalloproteinases-2 (TIMP-2) from Japanese puffer fishFugu rubripes, Fugu TIMP-2a and TIMP-2b, were cloned. The open reading frames of Fugu TIMP-2a and TIMP-2...In this study, genes of two distinct tissue inhibitors of metalloproteinases-2 (TIMP-2) from Japanese puffer fishFugu rubripes, Fugu TIMP-2a and TIMP-2b, were cloned. The open reading frames of Fugu TIMP-2a and TIMP-2b cDNAsare composed of 660 and 657 nucleotides and 220 and 219 amino acids, respectively. Both Fugu TIMP-2s contain 12 cysteineresidues, which might form six disulfide bonds as in other animals’ TIMP-2s. Reverse-transcribed polymerase chain reactionanalysis showed the mRNAs of Fugu TIMP-2a and TIMP-2b to be expressed in some tissues examined with different expres-sion patterns. These findings suggest that the two distinct Fugu TIMP-2s might perform different functions in Fugu tissues.展开更多
Carson McCullers The Heart is a Lonely Hunter is her representative work. McCullers deploys the skill of fugue pattern through the multi-voices reflected in the characters, tactfully unite the form and content into a ...Carson McCullers The Heart is a Lonely Hunter is her representative work. McCullers deploys the skill of fugue pattern through the multi-voices reflected in the characters, tactfully unite the form and content into a whole. By adopting Bakhtin's theory of polyphony, this paper aims to analyze the fugue pattern together with its multi-voices in the three parts, namely, the exposition, the development and the return reflected in the novel, which highlights its theme of universal loneliness so impressively.展开更多
为开展黄鳍东方鲀（Fugu xanthopterus）批量全人工育苗、胚胎及仔稚幼鱼发育特征研究,作者使用地欧酮（DOM）、促黄体释放激素类似物（LRH）及绒毛膜促性腺激素（HCG）对性成熟黄鳍东方鲀进行人工催产。结果表明黄鳍东方鲀亲鱼效应时间...为开展黄鳍东方鲀（Fugu xanthopterus）批量全人工育苗、胚胎及仔稚幼鱼发育特征研究,作者使用地欧酮（DOM）、促黄体释放激素类似物（LRH）及绒毛膜促性腺激素（HCG）对性成熟黄鳍东方鲀进行人工催产。结果表明黄鳍东方鲀亲鱼效应时间为39-60h,获产率为55.0%-92.5%；卵受精率为35.5%-84.7%,3年产卵7400 g；受精卵在海水水温17.0-23.2℃、盐度21-30、pH8.0-8.2人工条件下孵化,孵化率为33.6%-91.7%,孵出仔鱼499万尾；2005年、2006年经前期培育,共培育出黄鳍东方鲀后期仔鱼394.5万尾,成活率为68.2%-94.3%；室内水泥池后期培育出平均全长2.6 cm±0.2 cm幼鱼3100尾,成活率为15.5%；池塘生态培育出平均全长3.7 cm±0.5 cm幼鱼53.3万尾,成活率为11.2%-13.8%。黄鳍东方鲀受精卵为沉性黏性卵,近椭球形,乳白色,卵膜厚且不透明,卵径0.9-1.0mm,在海水水温18.2-22.2℃条件下,3 h分裂为2细胞；3 h 20 min 4细胞；3 h 45 min 8细胞；10 h 10 min多细胞；17 h 30 min囊胚出现；28 h原肠期；57 h 15 min胚体形成期；69 h 20 min心跳出现；130 h 50 min出膜期；144 h孵出仔鱼。初孵仔鱼全长2.2-2.4 mm,体圆而粗短,尾部细长、透明；孵出后第20天,胸鳍、臀鳍、背鳍基本形成,进入稚鱼期；孵出后第45天,上下颌门齿发达,各鳍发育完整,小刺基本形成,进入幼鱼期。展开更多
基金The finance special program of Tianjinthe transformation project of Tianjin Agricultural Achievements
文摘The ovary is an excellent system for studying stem cell renewal and differentiation, which is under the control of ovarian somatic cells. In order to understand oogenesis in Fugu rubripes （Temminck et. Schlegel） as a marine fish model of aquaculture importance, we established cell lines called TSOC 1 and TSOC2 from a juvenile ovary of this organism. TSOC1 is composed of spindle epithelial-like cells, while the other is cobblestone-like ceils. Therefore, TSOC1 and TSOC2 appear to consist of ovarian somatic cells. Growth requirement condition was investigated including temperature, concentration of FBS and pH. Significant fluorescent signals were observed after TSOC1 and TSOC2 cells were transfected with pEGFP-N3 vector, indicating its potential utility for genetic manipulation such as gene function studies. It is shown that these cell lines are effective for infection by the turbot reddish body iridovirus and flounder lymphosystis disease virus as evidenced by the appearance of cytopathic effect and virus propagation in the virus-infected cells, and most convincingly, the observation of viral particles by electron microscopy, demonstrating that TSOC1 and TSOC2 are suitable to study interactions between virus and host cells. It is believed that TSOC1 and TSOC2 will be useful tools to study sex-related events and interactions between primordial germ cells and oogonia cells during oogenesis. Therefore, establishment of ovary cell lines from Fugu rubripes seems to be significant for those research areas.
文摘Based on the Leaderman constitutive relations in nonlinear viscoelasticity and the linear geometrical assumption, a mathematical model for the bending of nonlinear viscoelastic beams was established in this paper. The Laplace transformation method and the Titchmarsh theorem were used to prove that some relations exist between solutions to bending problems of visco- and elastic beams, which reveals the fugue effect of viscoelastic materials. The high-order Galerkin approximate solution to the quasi-static response of nonlinear viscoelastic beams under a step load was obtained by using the new method suggested in this paper as well as the Mathematica software and the Newton iteration technique.
基金partially supported by a grant from the Ministry of Education,Culture,Sports,Science and Technology of Japan.
文摘In this study, genes of two distinct tissue inhibitors of metalloproteinases-2 (TIMP-2) from Japanese puffer fishFugu rubripes, Fugu TIMP-2a and TIMP-2b, were cloned. The open reading frames of Fugu TIMP-2a and TIMP-2b cDNAsare composed of 660 and 657 nucleotides and 220 and 219 amino acids, respectively. Both Fugu TIMP-2s contain 12 cysteineresidues, which might form six disulfide bonds as in other animals’ TIMP-2s. Reverse-transcribed polymerase chain reactionanalysis showed the mRNAs of Fugu TIMP-2a and TIMP-2b to be expressed in some tissues examined with different expres-sion patterns. These findings suggest that the two distinct Fugu TIMP-2s might perform different functions in Fugu tissues.
文摘Carson McCullers The Heart is a Lonely Hunter is her representative work. McCullers deploys the skill of fugue pattern through the multi-voices reflected in the characters, tactfully unite the form and content into a whole. By adopting Bakhtin's theory of polyphony, this paper aims to analyze the fugue pattern together with its multi-voices in the three parts, namely, the exposition, the development and the return reflected in the novel, which highlights its theme of universal loneliness so impressively.
文摘为开展黄鳍东方鲀（Fugu xanthopterus）批量全人工育苗、胚胎及仔稚幼鱼发育特征研究,作者使用地欧酮（DOM）、促黄体释放激素类似物（LRH）及绒毛膜促性腺激素（HCG）对性成熟黄鳍东方鲀进行人工催产。结果表明黄鳍东方鲀亲鱼效应时间为39-60h,获产率为55.0%-92.5%；卵受精率为35.5%-84.7%,3年产卵7400 g；受精卵在海水水温17.0-23.2℃、盐度21-30、pH8.0-8.2人工条件下孵化,孵化率为33.6%-91.7%,孵出仔鱼499万尾；2005年、2006年经前期培育,共培育出黄鳍东方鲀后期仔鱼394.5万尾,成活率为68.2%-94.3%；室内水泥池后期培育出平均全长2.6 cm±0.2 cm幼鱼3100尾,成活率为15.5%；池塘生态培育出平均全长3.7 cm±0.5 cm幼鱼53.3万尾,成活率为11.2%-13.8%。黄鳍东方鲀受精卵为沉性黏性卵,近椭球形,乳白色,卵膜厚且不透明,卵径0.9-1.0mm,在海水水温18.2-22.2℃条件下,3 h分裂为2细胞；3 h 20 min 4细胞；3 h 45 min 8细胞；10 h 10 min多细胞；17 h 30 min囊胚出现；28 h原肠期；57 h 15 min胚体形成期；69 h 20 min心跳出现；130 h 50 min出膜期；144 h孵出仔鱼。初孵仔鱼全长2.2-2.4 mm,体圆而粗短,尾部细长、透明；孵出后第20天,胸鳍、臀鳍、背鳍基本形成,进入稚鱼期；孵出后第45天,上下颌门齿发达,各鳍发育完整,小刺基本形成,进入幼鱼期。
