The response surface methodology(RSM) combined with bioassays was employed to optimize the extraction process of crude fucose-containing sulphated polysaccharides(c FCSP) from Sargassum fusiforme. The central composit...The response surface methodology(RSM) combined with bioassays was employed to optimize the extraction process of crude fucose-containing sulphated polysaccharides(c FCSP) from Sargassum fusiforme. The central composite design(CCD) was used with four variables, five levels, and four responses. The four variables were p H value of hydrochloric acid solution, extraction temperature(℃), ratio of liquid to raw material(m L g^(-1)), and extraction time(h), respectively. Chemical and bioassay indices were used in combination as the response parameters, which included the yield of c FCSP, fucose content, proliferation rate of spleen cells, and lipopolysaccharide-induced proliferation of splenocytes. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis, and examined using the appropriate statistical methods. The best extraction conditions were as follows: the p H value of hydrochloric acid solution was 3.50; the extraction temperature was 100℃; the ratio of liquid to raw material was 15.00 m L g^(-1) and the extraction time was 2.50 h. The experimental yield was close to the predicted from the model. The extract could promote spleen lymphocyte proliferation, especially the lipopolysaccharide-induced lymphocyte proliferation in vitro, which suggested that its immunomodulatory effect on B lymphocytes. Therefore, c FCSP extracted from S. fusiforme could be utilized as an immunostimulant in functional foods and pharmaceutical industry in future.展开更多
Fucose-containing oligosaccharides exhibit various biological activities,particularly for improving the intestinal health of infants.Bacterial exopolysaccharides are promising resource of fucose-containing oligosaccha...Fucose-containing oligosaccharides exhibit various biological activities,particularly for improving the intestinal health of infants.Bacterial exopolysaccharides are promising resource of fucose-containing oligosaccharides.A fucose-containing trisaccharide(_(D-)Glcp-β-(1→4)_(-L)-Fucp and _(D-)Galp-α-(1→3)_(-L)-Fucp,GuFGa)with same monosaccharide composition with 2′-fucosyllactose(2′-FL,the most abundant oligosaccharide in human milk),is obtained from bacterial exopolysaccharides.This study compared the in vitro prebiotic activities of GuFGa and 2′-FL using mono-and co-culture fermentation.Mono-culture shows GuFGa could be utilized by Bifidobacterium infantis and Lactobacillus plantarum,while 2′-FL only promote the proliferation of B.infantis.Co-culture fermentation involves cross-feeding between B.infantis and L.plantarum to utilize GuFGa.Further in vitro fecal microbiota fermentation of breastfed infants demonstrates that GuFGa exhibits better prebiotic activity than 2′-FL in increasing beneficial bacteria(Bifidobacterium and Lactobacillus)abundance and reducing harmful bacteria(Clostridium_sensu_stricto and Escherichia-Shigella)abundance.Moreover,GuFGa and 2′-FL fermentation shifted the gut microbiota composition of formula-fed infants to that of breastfed infants.Furthermore,herein,GuFGa and 2′-FL caused distinct metabolic profiles in both short-chain fatty acids and indole derivatives(e.g.indolelactic acid and indolepropionic acid).In summary,this study provides insights into the exploration of novel functional fucose-containing oligosaccharides as potential prebiotics.展开更多
The deoxyhexose sugar L-fucose and fucosylated oligosaccharides(FCO)are essential for various biological processes in human body and associated gut microbiota.Bacterial exopolysaccharides offer a promising new source ...The deoxyhexose sugar L-fucose and fucosylated oligosaccharides(FCO)are essential for various biological processes in human body and associated gut microbiota.Bacterial exopolysaccharides offer a promising new source for preparing functional oligosaccharides.Here,FCO were obtained through acidolysis of fucosecontaining exopolysaccharides with a molecular weight of 5.9×10^(3) kDa,derived from Clavibacter michiganensis M2.Structural analysis revealed that the main disaccharide components of FCO exhibited a molecular weight of 326 Da and a monosaccharide composition of fucose,galactose,and glucose in a 3:1:2 ratio.Nuclear magnetic resonance indicated that the disaccharide fragments consisted of two backbones of α-Galp-(1→2)-α-Fucp andβ-Glcp-(1→4)-β-Fucp,respectively.In vitro fermentation of FCO with human fecal cultures resulted in an increased abundance of Bifidobacterium and Bacteroides,and a reduction in Escherichia-Shigella and Fusobacterium.This research outlines a novel structural fucosylated oligosaccharide and its preparation method,suggesting its potential application as a promising new prebiotic.展开更多
基金supported by the National High Technology Research and Development Program of China (863 Program) (2013AA093003)
文摘The response surface methodology(RSM) combined with bioassays was employed to optimize the extraction process of crude fucose-containing sulphated polysaccharides(c FCSP) from Sargassum fusiforme. The central composite design(CCD) was used with four variables, five levels, and four responses. The four variables were p H value of hydrochloric acid solution, extraction temperature(℃), ratio of liquid to raw material(m L g^(-1)), and extraction time(h), respectively. Chemical and bioassay indices were used in combination as the response parameters, which included the yield of c FCSP, fucose content, proliferation rate of spleen cells, and lipopolysaccharide-induced proliferation of splenocytes. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis, and examined using the appropriate statistical methods. The best extraction conditions were as follows: the p H value of hydrochloric acid solution was 3.50; the extraction temperature was 100℃; the ratio of liquid to raw material was 15.00 m L g^(-1) and the extraction time was 2.50 h. The experimental yield was close to the predicted from the model. The extract could promote spleen lymphocyte proliferation, especially the lipopolysaccharide-induced lymphocyte proliferation in vitro, which suggested that its immunomodulatory effect on B lymphocytes. Therefore, c FCSP extracted from S. fusiforme could be utilized as an immunostimulant in functional foods and pharmaceutical industry in future.
基金supported by the National Natural Science Foundation of China(31872893 and 32202064)China Postdoctoral Science Foundation(2021M701547)+1 种基金Fundamental Research Funds for the Central Universities(202261043)Natural Science Foundation of Jiangxi Province(20224BAB215044).
文摘Fucose-containing oligosaccharides exhibit various biological activities,particularly for improving the intestinal health of infants.Bacterial exopolysaccharides are promising resource of fucose-containing oligosaccharides.A fucose-containing trisaccharide(_(D-)Glcp-β-(1→4)_(-L)-Fucp and _(D-)Galp-α-(1→3)_(-L)-Fucp,GuFGa)with same monosaccharide composition with 2′-fucosyllactose(2′-FL,the most abundant oligosaccharide in human milk),is obtained from bacterial exopolysaccharides.This study compared the in vitro prebiotic activities of GuFGa and 2′-FL using mono-and co-culture fermentation.Mono-culture shows GuFGa could be utilized by Bifidobacterium infantis and Lactobacillus plantarum,while 2′-FL only promote the proliferation of B.infantis.Co-culture fermentation involves cross-feeding between B.infantis and L.plantarum to utilize GuFGa.Further in vitro fecal microbiota fermentation of breastfed infants demonstrates that GuFGa exhibits better prebiotic activity than 2′-FL in increasing beneficial bacteria(Bifidobacterium and Lactobacillus)abundance and reducing harmful bacteria(Clostridium_sensu_stricto and Escherichia-Shigella)abundance.Moreover,GuFGa and 2′-FL fermentation shifted the gut microbiota composition of formula-fed infants to that of breastfed infants.Furthermore,herein,GuFGa and 2′-FL caused distinct metabolic profiles in both short-chain fatty acids and indole derivatives(e.g.indolelactic acid and indolepropionic acid).In summary,this study provides insights into the exploration of novel functional fucose-containing oligosaccharides as potential prebiotics.
基金supported by the National Natural Science Foundation of China(32202064)Jiangxi Provincial Natural Science Foundation(20224BAB215044).
文摘The deoxyhexose sugar L-fucose and fucosylated oligosaccharides(FCO)are essential for various biological processes in human body and associated gut microbiota.Bacterial exopolysaccharides offer a promising new source for preparing functional oligosaccharides.Here,FCO were obtained through acidolysis of fucosecontaining exopolysaccharides with a molecular weight of 5.9×10^(3) kDa,derived from Clavibacter michiganensis M2.Structural analysis revealed that the main disaccharide components of FCO exhibited a molecular weight of 326 Da and a monosaccharide composition of fucose,galactose,and glucose in a 3:1:2 ratio.Nuclear magnetic resonance indicated that the disaccharide fragments consisted of two backbones of α-Galp-(1→2)-α-Fucp andβ-Glcp-(1→4)-β-Fucp,respectively.In vitro fermentation of FCO with human fecal cultures resulted in an increased abundance of Bifidobacterium and Bacteroides,and a reduction in Escherichia-Shigella and Fusobacterium.This research outlines a novel structural fucosylated oligosaccharide and its preparation method,suggesting its potential application as a promising new prebiotic.
