Food breakdown during storage and transit greatly adds to worldwide food waste.Biodegradable edible coatings derived from natural sources provide a sustainable method to extend the shelf life and preserve the freshnes...Food breakdown during storage and transit greatly adds to worldwide food waste.Biodegradable edible coatings derived from natural sources provide a sustainable method to extend the shelf life and preserve the freshness of fresh fruit.This study explores the potential of the readily available and nutrient-rich seaweed,Caulerpa lentillifera,as a base material for edible coatings that can enhance the shelf life and maintain the physicochemical properties of fruits and vegetables.Caulerpa lentillifera,a marine macroalga renowned for its unique biochemical composition,presents a promising avenue for developing sustainable bio-coatings to improve the post-harvest quality of fresh produce.Biodegradable edible coatings derived from natural sources provide a sustainable method to extend the shelf life and preserve the freshness of fresh fruit.This study is aimed at exploring the potential of Caulerpa lentillifera,a nutrient-dense green seaweed,as a foundational material for creating edible coatings to enhance the post-harvest quality of fruits and vegetables.Various Caulerpa lentillifera-derived coating formulations were developed and applied to specific fruits and vegetables.The coated samples were maintained under controlled conditions and assessed for alterations in physicochemical parameters,including weight loss,hardness,colour,and microbial proliferation.The optimised coating formulation markedly diminished weight loss,postponed ripening,and maintained firmness and colour relative to uncoated controls.The coating demonstrated promising antibacterial properties,aiding in the extension of shelf life.Coatings derived from Caulerpa lentillifera offer a viable,environmentally sustainable alternative to synthetic preservatives,facilitating the advancement of sustainable food preservation technology.展开更多
This study was designed to determine factors that influence market performance of avocado fruits among smallholder farmers in Mbeya and Songwe regions. A cross section research design entailing 209 farmers from five (...This study was designed to determine factors that influence market performance of avocado fruits among smallholder farmers in Mbeya and Songwe regions. A cross section research design entailing 209 farmers from five (5) districts of two (2) regions and 15 key informants;5 local service providers (LSP), 5 traders/brokers and 5 district extension officers (DEO) were involved in the study. Random selection procedures were used to select avocado producers and purposive selection procedures were used to select key informants. The study employed survey questionnaires to collect data from avocado farmers and structured interview to collect data from key informants. Collected data were analyzed with an aid of descriptive statistics and linear regression model was used to determine significant factors that determine market prices of avocado fruits among smallholder farmers. The demographic characteristics of avocado farmers showed that;61.2% of farmers had age between 36 to 59 years old, 84.7% were married, 74.6% had primary education level, average number of 5 members of the family, average 4.3 acres of owned land and 1.8 acres planted with avocado. Moreover, findings showed that 90.9% grew improved avocado varieties. Farm gate (88.5%) was the major market outlet chosen by avocado farmers. Local merchants and processors were the major buyers of the produced avocado. Results from linear regression analysis showed that variety grown (P P P P < 0.05) were factors that statistically influenced market prices for avocado fruits. The study concluded that despite the remarkable contributions to livelihood of the farmers yet, it is highly fragile on market prices. Therefore, the study recommends the need to establish market systems that will govern avocado fruits marketing for sustainability of the sub-sector.展开更多
The content of soluble sugars is a vital parameter that indicates the quality of fleshy fruits such as apple(Malus domestica Borkh.).Studying the patterns of accumulation of soluble sugars and regulatory mechanisms as...The content of soluble sugars is a vital parameter that indicates the quality of fleshy fruits such as apple(Malus domestica Borkh.).Studying the patterns of accumulation of soluble sugars and regulatory mechanisms associated with fruit development is crucial for breeding improved fruit varieties.Here,we report that MdCIbHLH1,a low temperature-induced b HLH transcription factor,inhibits the accumulation of soluble sugars by regulating sugar-metabolizing enzyme activities,photosynthetic performance,and the expression of sugar-related genes in developing apple fruits.MdCIbHLH1 inhibits MdFBP and MdPEPCK expression,thus blocking the conversion of acids to sugars in apple fruits.We also discovered that MdCIbHLH1 decreases the photosynthetic rate and carbohydrate accumulation in apple leaves.Our results suggest that soluble sugar accumulation in apple fruits is influenced by multiple factors,including metabolic status,photosynthesis,and carbohydrate allocation.MdCIbHLH1 is critically involved in controlling the accumulation of soluble sugars by coordinating carbohydrate synthesis and allocation,thus influencing sugar transport and its metabolism during the development of apple fruits.展开更多
The WSC proteins produced by Penicillium expansum play a crucial role in causing blue mold on pears.To analyze the role of the WSC1 gene in the pathogenic process of this fungal pathogen,we conducted transcriptomic an...The WSC proteins produced by Penicillium expansum play a crucial role in causing blue mold on pears.To analyze the role of the WSC1 gene in the pathogenic process of this fungal pathogen,we conducted transcriptomic analysis of a WSC1 knockout(ΔWSC1)strain.The knockout of WSC1 significantly altered the gene expression profile in P.expansum,particularly for genes involved in cell wall integrity,signaling,stress response,and toxin production.The differential expression of these genes might make theΔWSC1 strain more vulnerable to environmental stress,while reducing the toxin production capacity,ultimately leading to a decrease in the pathogenicity.The transcriptomic analysis revealed that the expression of genes related to stress response signals,defense mechanisms and oxidative stress management changed when pear fruits were infected with theΔWSC1 strain.These changes may trigger a cascade of responses in pear fruits.In addition,compared with those infected with the wild-type strain,pear fruits infected with theΔWSC1 strain exhibited up-regulated expression of genes related to defense and oxidative stress.This study clarifies how the WSC1 gene influences P.expansum’s ability to infect pear fruits and how pear fruits respond to the infection.展开更多
According to the requirements of agricultural production and usem, taking diagnosis and decision-making of prevention for common diseases and pests in fruits and vegetables in southern China as the core, with communic...According to the requirements of agricultural production and usem, taking diagnosis and decision-making of prevention for common diseases and pests in fruits and vegetables in southern China as the core, with communication and sharing as principle, adopted diagnosis, inquiries and guiding prevention of diseases and pests in fruits and vegetables as purpose, expert examination system of plant disease and pests in fruits and vegetables based on Web highly integrates the knowledge and prevention techniques of common diseases and pests for main fruit and vegetable in south China. In this system, the users can browse and inquiry the information about the fruit and vegetable diseases and pests, as well as their diagnosis and control. The implementation of the system plays an active role in promo- ting plant protection knowledge and guiding farms to scientifically control diseases and pests in fruits and vegetables展开更多
[Objective] This study aimed to compare and analyse the morphological characteristics of the seed-like fruits between Salvia shandongensis J. X. Li et F. Q. Zhou and its relatives. [Method] The morphological character...[Objective] This study aimed to compare and analyse the morphological characteristics of the seed-like fruits between Salvia shandongensis J. X. Li et F. Q. Zhou and its relatives. [Method] The morphological characteristics of the mature seed-like fruits of Salvia shandongensis and its three congeners, S. miltiorrhiza, S. miltiorrhiza f. alba and S. bowleyana, were comparatively observed using both optical microscope and scanning electron microscope. [Result] The results showed that there were distinct differences in the shape, size and exine sculpture of the seed-like fruits of the four Danshen congeners： the seed-like fruits of S. shandongensis that were elliptical and had areolate sculpture with subquadrangular convex meshes as well as densely filamentous transverse lines were easy to be distinguished from those of S. miltiorrhiza Bge., which were broadly elliptical and had reticulate sculpture with subquadrate concave meshes. The seed-like fruits of S. bowleyana were fusiform and their ektexine had reticulate sculpture with polygonal meshes, which were slightly convex at the center, which are obviously different from those of S. shandongensis. [Conclusion] This study first accumulates data of the seed-like fruits of S. shandongensis, which provides important bases for establishing its taxonomical status and studying its germplasm. The morphological characteristics and reticulate sculpture on ektexine of the seed-like fruits S. miltiorrhiza Bge. are consistent with the previous report. The results in this study not only provide references for uncovering the genetic relationships between S. shandongensis and S. miltiorrhiza Bge., as well as the interspecific classification and identification. In addition, this study also provides new scientific proofs for the identification of Danshen species and their introduction and cultivation, genetics and breeding.展开更多
BACKGROUND Fruits of Gardenia jasminoides(G.jasminoides)have extremely high medicinal value.However,the quality and traits of the plants vary significantly based on their provenances.In addition,the behaviour of the k...BACKGROUND Fruits of Gardenia jasminoides(G.jasminoides)have extremely high medicinal value.However,the quality and traits of the plants vary significantly based on their provenances.In addition,the behaviour of the known bioactive components,such as geniposide and crocin,has been the primary focus of the research on G.jasminoides.However,the identification of unknown bioactive components and their metabolomics remains underexplored.Therefore,analysing the metabolic differences between gardenias from different sources is essential to provide a comprehensive theoretical basis for the evaluation of G.jasminoides and germplasm resource identification.AIM To systematically evaluate the morphology,secondary metabolites,typical active ingredients,and antioxidant activity of wild G.jasminoides fruits.METHODS Gardenia fruits were collected from different provenances.Metabolites were identified via ultra-high-performance liquid chromatography–tandem mass spectrometry(UPLC-MS/MS).The metabolic differences were compared using hierarchical cluster analysis(HCA).The antioxidant capacity was evaluated using 2,2-diphenyl-1-picrylhydrazyl radical scavenging assays and ferric reducing antioxidant power assays,and its correlation with typical active ingredients was analysed.RESULTS A total of 444 and 240 metabolites were identified using UPLC-MS/MS in positive and negative ion modes,respectively.The HCA results of the flavonoids indicated that the higher content of flavonoids was in the fruits from Lukou.The differential analysis of metabolites in fruits from Shaoyang,Miluo and Lukou showed that the fruit from Miluo had the highest upregulated differential metabolites.CONCLUSION The metabolic characteristics of the Ningxiang and Xiangxi extracts were similar,while those of Lukou,Miluo and Shaoyang extracts differed significantly.展开更多
[Objective] This study aimed to identify red pigment of Panax notoginseng fruits and explore the correlation between pigment content and total saponins of the fruits. [Method] The red pigment of Panax notoginseng frui...[Objective] This study aimed to identify red pigment of Panax notoginseng fruits and explore the correlation between pigment content and total saponins of the fruits. [Method] The red pigment of Panax notoginseng fruits was preliminarily identi- fied with specific color reactions and UV-vis spectra, and the contents of the pigment and total saponins were determined via spectrophotometry. [Result] The red hues of the fruits were contributed by anthocyanins and/or the anthocyanidins. The contents of anthocyanins and total saponins of the fruits both decreased along with thinning of the red hues. The content difference of the anthocyanins in fruits with different red hues reached extremely significant level, but that of total saponins just reached significant level. [Conclusion] The red pigment of P. notoginseng fruits is anthocyanins which are of extremely significant positive correlation with total saponins in contents.展开更多
The increasing incidence of neurodegenerative diseases(NDs)and the constraints of existing treatment methods have spurred a keen interest in investigating alternative therapies.Medicinal plants,renowned for their long...The increasing incidence of neurodegenerative diseases(NDs)and the constraints of existing treatment methods have spurred a keen interest in investigating alternative therapies.Medicinal plants,renowned for their long-standing use in traditional medicine,offer a hopeful avenue for discovering new neuroprotective agents.This study emphasizes the potential neuroprotective characteristics of edible fruit plants in Bangladesh,specifically focusing on their traditional folk medicine uses for neurological disorders.This study provides an in-depth overview of the different types of edible fruit trees in Bangladesh and their phytochemicals,including flavonoids,terpenoids,and phenolic acids.This work examines the scientific data supporting the neuroprotective properties of bioactive chemicals from plants.It further explores the mechanisms by which these compounds work to counteract oxidative stress,decrease inflammation,and stimulate neurogenesis.Moreover,the study investigates toxicological characteristics and bioactive components of some fruits,emphasizing the importance of further investigation to measure their safety profile comprehensively.This thorough study highlights the potential benefits of Bangladesh's edible fruit trees as a rich source of neuroprotective chemicals.It also shows that additional research might lead to novel approaches for improving brain functioning and preventing NDs.展开更多
Plant polyphenols are potential substitutes for clinicalα-glucosidase inhibitors.Our previous studies indicated that prodelphinidins from Chinese bayberry leaves(BLPs)have strongerα-glucosidase inhibitory activity t...Plant polyphenols are potential substitutes for clinicalα-glucosidase inhibitors.Our previous studies indicated that prodelphinidins from Chinese bayberry leaves(BLPs)have strongerα-glucosidase inhibitory activity than other common polyphenols,but they have no safe history of consumption.There is a reasonable prospect that prodelphinidins from Chinese bayberry fruits(BFPs)can improve postprandial hyperglycemia,though known active components are only myricetin and cyanidin-3-O-glucoside.Hence,the aim of this study was to analyze structure and hypoglycemic effect of BFPs of“Biqi”(BQPs)and“Dongkui”(DKPs)cultivars,and compare their difference with BLPs.The contents of BQPs and DKPs were(221.73±0.60)and(136.82±4.33)mg epicatechin(EC)equivalents/g dry weight,respectively.The most subunits were epigallocatechin gallate along with a small amount of epigallocatechin,epicatechin gallate and EC connected by B-type linkages and a small portion of A-type linkages with mean polymerization degree of 4.25 and 4.08,respectively.Importantly,BQPs and DKPs wereα-glucosidase inhibitors with half inhibitory concentration of 11.91 and 9.47μg/m L respectively,which were significantly stronger than BLPs.DKPs could also improve postprandial hyperglycemia of normal mice and high fat diet-induced type II diabetic mice.Therefore,edible prodelphinidins,which have stronger hypoglycemic effect than BLPs,were first found in Chinese bayberry fruits.展开更多
The present study was carried out to make new healthy synbiotic flavored fermented skim milk drinks (SFFSD) supplemented with either Doum (Hyphaene thebaica L.) or Carob (Ceratonia siliqua) fruits powder for nutrition...The present study was carried out to make new healthy synbiotic flavored fermented skim milk drinks (SFFSD) supplemented with either Doum (Hyphaene thebaica L.) or Carob (Ceratonia siliqua) fruits powder for nutritional, antioxidant and antimicrobial activities. The HPLC analysis showed higher phenolic and flavonoid content in Doum extract than that of Carob. The antimicrobial activity of the methanolic extracts of both Doum and Carob against foodborne pathogens showed that the most sensitive indicators were Bacillus cereus, C. albicans and S. aureus, followed by T. mentagrophyte and E. coli wherein, the diameter of clear zones was, 29, 27, 24, 23 and 13 mm, respectively. While no effect was noticed against Aspergillus flavus. On the other hand, the methanolic extract of Carob fruit exhibited only a weak antibacterial effect against B. cereus (20 mm). Six treatments of SFFSD were made using both Doum or Carob fruit powder as prebiotic and Lactobacillus paracasei as probiotic bacteria. The added levels from Doum were 2%, 4% and 6%, while that added from Carob were 5%, 10% and 15%. Adding Doum and Carob powder increased the containing of minerals;Ca, K, Mg and iron compared with control. Both viscosity and viability of L. paracasei for the SFFSD were increased with increasing the added levels from either Doum or Carob, compared with the control. Samples of SFFSD containing Carob powder, show superior sensory for all parameters and total score points during storage period, especially at a level of 10%. It seems evident that samples supplemented with Doum powder were also acceptable.展开更多
This paper aimed to explore the major meteorological disasters impacts on the growth of Jujube fruits.By analyzing statistic characters of meteorological data and historic yield data of Jujube fruits,the influence of ...This paper aimed to explore the major meteorological disasters impacts on the growth of Jujube fruits.By analyzing statistic characters of meteorological data and historic yield data of Jujube fruits,the influence of different factors on the different growing stages of Jujube fruits was studied,in terms of temperature,precipitation,and relative humidity.The major meteorological disasters which impacted the growth of Jujube fruits were as follow:(1) Hot days when the temperature was equal to or above 30 ℃ continued over 9 days at full bloom stage;(2) The continuous rainy days lasted over 10 days at mature stage;(3) The continuous rainy days lasted over 7 days at full bloom stage;(4) Clod days when the temperature was equal to or below-22 ℃ continued over 4 days and the minimum temperature reached-24 ℃ at wintering stage.The major meteorological disasters which impact Jujube fruits were different at different stages.The most frequently occurred major meteorological disasters was the hot days when the temperature was equal to or above 30 ℃ and lasted over 9 days at full bloom stage.展开更多
Genetic manipulation of genes to upregulate specific branches of metabolic pathways is a method that is commonly used to improve fruit quality.However,the use of a single gene to impact several metabolic pathways is d...Genetic manipulation of genes to upregulate specific branches of metabolic pathways is a method that is commonly used to improve fruit quality.However,the use of a single gene to impact several metabolic pathways is difficult.Here,we show that overexpression of the single gene SlMYB75(SlMYB75-OE)is effective at improving multiple fruit quality traits.In these engineered fruits,the anthocyanin content reached 1.86mg g−1 fresh weight at the red-ripe stage,and these SlMYB75-OE tomatoes displayed a series of physiological changes,including delayed ripening and increased ethylene production.In addition to anthocyanin,the total contents of phenolics,flavonoids and soluble solids in SlMYB75-OE fruits were enhanced by 2.6,4,and 1.2 times,respectively,compared to those of wild-type(WT)fruits.Interestingly,a number of aroma volatiles,such as aldehyde,phenylpropanoid-derived and terpene volatiles,were significantly increased in SlMYB75-OE fruits,with some terpene volatiles showing more than 10 times higher levels than those in WT fruits.Consistent with the metabolic assessment,transcriptomic profiling indicated that the genes involved in the ethylene signaling,phenylpropanoid and isoprenoid pathways were greatly upregulated in SlMYB75-OE fruits.Yeast one-hybrid and transactivation assays revealed that SlMYB75 is able to directly bind to the MYBPLANT and MYBPZM cis-regulatory elements and to activate the promoters of the LOXC,AADC2 and TPS genes.The identification of SlMYB75 as a key regulator of fruit quality attributes through the transcriptional regulation of downstream genes involved in several metabolic pathways opens new avenues towards engineering fruits with a higher sensory and nutritional quality.展开更多
Peach fruits [Prumus persica （L.） Batsch, cv. Yuhuasanhao] were used as materials to investigate the changes of reactive oxygen species （ROS） and related enzymes in mitochondria respiration during storage and then...Peach fruits [Prumus persica （L.） Batsch, cv. Yuhuasanhao] were used as materials to investigate the changes of reactive oxygen species （ROS） and related enzymes in mitochondria respiration during storage and then their influence on senescence of harvested Peach fruits was studied. The results showed that low temperature （5℃） strongly inhibited the reduction of firmness and the increase in respiration rate. During storage at ambient temperature （20℃）, ROS had a cumulative process while malondialdehye （MDA） content continued to increase in associated with enhanced membrane lipid peroxidation. Lipoxygenase （LOX） activity was strongly inhibited under the low temperature condition. The activities of succinic dehydrogenase （SDH）, cytochrome C oxidase （CCO）, and Ca^2＋-ATPase declined to a certain extent at ambient temperature, while they showed higher activities at low temperature, which may be related to lower membrane lipid peroxidation at low temperature. Higher Ca^2＋ content at ambient temperature may be responsible for impairment of mitochondrial function, thus, leading to fruit senescence. The results showed that under low temperature condition, the low accumulation of ROS and the low level of membrane lipid peroxidation could maintain the function of mitochondria that would help to delay the senescence of peach fruits. These suggested a close relationship existed between ROS metabolism and mitochondrial respiration. It can be inferred that the low temperature helps to delay senescence of peach fruits via suppression of ROS and related enzymes, maintain better homeostasis of Ca^2＋ in mitochondria and thus better mitochondrial functions.展开更多
Objective:To investigate the anti-inflammatory effects and the action mechanism of the fruits of Horenia dulcis(H.dulcis) in lipopolysaccharide(LPS)-induced mouse macrophage Raw 264.7cells.Methods:The extract of H.dul...Objective:To investigate the anti-inflammatory effects and the action mechanism of the fruits of Horenia dulcis(H.dulcis) in lipopolysaccharide(LPS)-induced mouse macrophage Raw 264.7cells.Methods:The extract of H.dulcis fruits(EHDF) were extracted with 70%ethanol.Mouse macrophages were treated with different concentrations of EHDF in the presence and absence of LPS(1 μg/mL).To demonstrate the inflammatory mediators including nitric oxide,inducible nitric oxide synthase and cyclooxygenase(COX)-2 expression levels were analyzed by usingin vitro assay systems.COX-derived pro-inflammatory cytokines including interleukin-1 β.tumor necrosis factor- α and prostaglandin F_2 were determined using ELISA kits.Cell viability,heme oxygenase-1 expression,nuclear factor-kappaB and nuclear factor F.2-related factors 2 translocation were also investigated.Results:EHDF potently inhibited the LPS-stimulated nitric oxide,inducible nitric oxide synthase.COX-2,interleukin-1 β and tumor necrosis factor- α expression in a dose-dependent manner.EHDF suppressed the phosphorylation of inhibited kappaB-alpha and p65 nuclear translocation.Treatment of macrophage cells with EHDF alone induced the heme oxygenase-1 and nuclear translocation of nuclear factor E2-reIated factor 2.Conclusions:These results suggest that the ethanol extract of H.dulcis fruit exerts its anti-inflammatory effects by inhibiting inhibited kappaBalpha phorylation and nuclear translocation of nuclear factor-kappaB.展开更多
In this paper,we report the construction of two accurate mass databases and the development of a combination detection method that simultaneously screens for 733 pesticide and chemical contaminant multi-residues via h...In this paper,we report the construction of two accurate mass databases and the development of a combination detection method that simultaneously screens for 733 pesticide and chemical contaminant multi-residues via high-throughput liquid chromatography(LC)-and gas chromatography(GC)-quadru pole-time-of-flight mass spectrometry(Q-TOFMS).This work demonstrates that electronic mass spectral standards may replace chemical-source standard materials as references through one sample preparation and the combination of GC/LC-Q-TOFMS screening.This cutting-edge technique has also replaced multiresidue determination using targeted detection with non-targeted screening.The pesticide residue types,sensitivity,recovery,and reproducibility of this combination technique are evaluated in eight fruit and vegetable matrices.This technique shows three advantages:①In comparison with the discovery capability of a single technique,the combination technique shows an improvement of 51.1%(GC-QTOFMS)and 39.6%(LC-Q-TOFMS),respectively;②the combination technique can satisfy a screening limit lower than 10μg·kg^-1 and meet the requirements of“uniform standards,”although some of the pesticide residues could be optimized to further improve screening sensitivity;③over 488 pesticides with recoveries between 60%-120%and relative standard deviation(RSD)<20%at a spiked level of 10μg·kg^-1 were detected with the combination technique in eight different matrices.From 2012 to 2017,this combination technique was applied in an investigation to screen pesticide residues from 1384 sampling locations for 38138 batched samples covering 18 categories and 134 types of fruits and vegetables obtained from across the mainland of China.After statistical analysis,533 pesticides in 115891 determinations were detected,and the regularity of pesticides in the fruits and vegetables sold on the Chinese market was shown.展开更多
To assess levels of contamination and human health risk, we analyzed the concentrations of the heavy metals lead （Pb）, cadmium （Cd）, chromium （Cr）, and nickel （Ni） in China’s main deciduous fruits-apple, pear...To assess levels of contamination and human health risk, we analyzed the concentrations of the heavy metals lead （Pb）, cadmium （Cd）, chromium （Cr）, and nickel （Ni） in China’s main deciduous fruits-apple, pear, peach, grape, and jujube. The concentration order of the heavy metals was Ni〉Cr〉Pb〉Cd. In 97.5% of the samples, heavy metal concentrations were within the maximum permissible limits. Among the fruits studied, the heavy metal concentrations in jujube and peach proved to be the highest, and those in grape proved to be the lowest. Only 2.2% of the samples were poluted by Ni, only 0.4% of the samples were poluted by Pb, and no samples were poluted by Cd or Cr. Compared with the other fruits, the combined heavy metal polution was signiifcantly higher （P〈0.05） in peach and signiifcantly lower （P〈0.05） in grape. For the combined heavy metal polution, 96.9% of the samples were at safe level, 2.32% at warning level, 0.65% at light level, and 0.13% at moderate level. In the fruits studied, the contribution of heavy metals to the daily intake rates （DIR） folowed the order of Ni〉Cr〉Pb〉Cd. The highest DIR came from apple, while the lowest DIR came from grape. For each of the heavy metals, the total DIR from ifve studied fruits corresponded to no more than 1.1% of the tolerable daily intake, indicating that no signiifcant adverse health effects are expected from the heavy metals and the fruits studied. The target hazard quotients and the total target hazard quotients demonstrated that none of the analyzed heavy metals may pose risk to consumers through the fruits studied. The highest risk was posed by apple, folowed in decreasing order by peach and pear, jujube, and grape. We suggest that the main deciduous fruits （apple, pear, peach, grape, and jujube） of China’s main producing areas are safe to eat.展开更多
Rhizopus rot of peach fruits could be significantly suppressed by Pichia membranefaciens. Polyphenol oxidase (PPO), peroxidase (POD) and phenylalanine ammonium-lyase (PAL) activities induced by inoculation with P. mem...Rhizopus rot of peach fruits could be significantly suppressed by Pichia membranefaciens. Polyphenol oxidase (PPO), peroxidase (POD) and phenylalanine ammonium-lyase (PAL) activities induced by inoculation with P. membrane faciens or R. stolonifer were studied in postharvest peach fruits. The activities of PPO and PAL in peaches increased significantly after being inoculated with P. membrane faciens + R. stolonifer by 24 h, the activities maintained at a high level throughout the experiment. Under the condition of infected with R. stolonifer alone, activity of PPO and PAL could also increased, but the levels were lower than those treated with P. membrane faciens+ R. stolonifer. However, fruits inoculaed with P. membrane-faciens + R. stolonifer or R. stolonifer alone did not stimulated POD activity. The results suggest that the activation of these defense enzymes is involved in the action of P. membrane faciens against R. stolonifer.展开更多
In this study, 15 mycotoxins were detected in 233 nut and dried fruit samples from China. The 15 mycotoxins included aflatoxins （AFs： AFB1, AFB2, AFG1 and AFG2）, trichothecene toxins （TCs： T-2, ZEA, ENA, ENA1, EN...In this study, 15 mycotoxins were detected in 233 nut and dried fruit samples from China. The 15 mycotoxins included aflatoxins （AFs： AFB1, AFB2, AFG1 and AFG2）, trichothecene toxins （TCs： T-2, ZEA, ENA, ENA1, ENB, ENB1 and BEA）, Alternaria toxins （ATs： TEN, AOH and AME） and ochratoxin A （OTA）. The mycotoxins were detected in 47.6% of the samples and all 15 of the mycotoxins were found. Two samples were positive for AFB1 and exceeded the maximum tolerable levels allowed in China. The contamination levels of the mycotoxins found in nuts, dried jujubes, raisins, dried figs and dried Iongans were in the ranges of 0.1-462.7, 0.2-247.3, 0.8-10.1,0.2-384.1 and 0.1-89.2 μg kg^-1, respectively. Dried figs （80.0%） had the highest incidence of mycotoxins, followed by dried Iongans （60.0%）, dried jujubes （57.1%）, nuts （43.6%） and raisins （26.7%）. The estimated daily intake （EDI） values of each individual mycotoxin and all of the mycotoxins collectively were calculated by both the deterministic approach （DA） and the probability approach （PA）. For risk characterization, dietary exposure to TCs, ATs and OTA through consumption of nuts and dried fruits according to both approaches, showed no health risk to Chinese adults by exposure to either individual mycotoxins or in combination. To the best of our knowledge, this is the first work in which risk assessment of multimycotoxins is performed, specifically including the emerging ENNs and BEA, in nuts and dried fruits of China.展开更多
文摘Food breakdown during storage and transit greatly adds to worldwide food waste.Biodegradable edible coatings derived from natural sources provide a sustainable method to extend the shelf life and preserve the freshness of fresh fruit.This study explores the potential of the readily available and nutrient-rich seaweed,Caulerpa lentillifera,as a base material for edible coatings that can enhance the shelf life and maintain the physicochemical properties of fruits and vegetables.Caulerpa lentillifera,a marine macroalga renowned for its unique biochemical composition,presents a promising avenue for developing sustainable bio-coatings to improve the post-harvest quality of fresh produce.Biodegradable edible coatings derived from natural sources provide a sustainable method to extend the shelf life and preserve the freshness of fresh fruit.This study is aimed at exploring the potential of Caulerpa lentillifera,a nutrient-dense green seaweed,as a foundational material for creating edible coatings to enhance the post-harvest quality of fruits and vegetables.Various Caulerpa lentillifera-derived coating formulations were developed and applied to specific fruits and vegetables.The coated samples were maintained under controlled conditions and assessed for alterations in physicochemical parameters,including weight loss,hardness,colour,and microbial proliferation.The optimised coating formulation markedly diminished weight loss,postponed ripening,and maintained firmness and colour relative to uncoated controls.The coating demonstrated promising antibacterial properties,aiding in the extension of shelf life.Coatings derived from Caulerpa lentillifera offer a viable,environmentally sustainable alternative to synthetic preservatives,facilitating the advancement of sustainable food preservation technology.
文摘This study was designed to determine factors that influence market performance of avocado fruits among smallholder farmers in Mbeya and Songwe regions. A cross section research design entailing 209 farmers from five (5) districts of two (2) regions and 15 key informants;5 local service providers (LSP), 5 traders/brokers and 5 district extension officers (DEO) were involved in the study. Random selection procedures were used to select avocado producers and purposive selection procedures were used to select key informants. The study employed survey questionnaires to collect data from avocado farmers and structured interview to collect data from key informants. Collected data were analyzed with an aid of descriptive statistics and linear regression model was used to determine significant factors that determine market prices of avocado fruits among smallholder farmers. The demographic characteristics of avocado farmers showed that;61.2% of farmers had age between 36 to 59 years old, 84.7% were married, 74.6% had primary education level, average number of 5 members of the family, average 4.3 acres of owned land and 1.8 acres planted with avocado. Moreover, findings showed that 90.9% grew improved avocado varieties. Farm gate (88.5%) was the major market outlet chosen by avocado farmers. Local merchants and processors were the major buyers of the produced avocado. Results from linear regression analysis showed that variety grown (P P P P < 0.05) were factors that statistically influenced market prices for avocado fruits. The study concluded that despite the remarkable contributions to livelihood of the farmers yet, it is highly fragile on market prices. Therefore, the study recommends the need to establish market systems that will govern avocado fruits marketing for sustainability of the sub-sector.
基金supported by grants from the National Natural Science Foundation of China(Grant Nos.32122080,31972375)Shandong Province(Grant No.ZR2020YQ25)。
文摘The content of soluble sugars is a vital parameter that indicates the quality of fleshy fruits such as apple(Malus domestica Borkh.).Studying the patterns of accumulation of soluble sugars and regulatory mechanisms associated with fruit development is crucial for breeding improved fruit varieties.Here,we report that MdCIbHLH1,a low temperature-induced b HLH transcription factor,inhibits the accumulation of soluble sugars by regulating sugar-metabolizing enzyme activities,photosynthetic performance,and the expression of sugar-related genes in developing apple fruits.MdCIbHLH1 inhibits MdFBP and MdPEPCK expression,thus blocking the conversion of acids to sugars in apple fruits.We also discovered that MdCIbHLH1 decreases the photosynthetic rate and carbohydrate accumulation in apple leaves.Our results suggest that soluble sugar accumulation in apple fruits is influenced by multiple factors,including metabolic status,photosynthesis,and carbohydrate allocation.MdCIbHLH1 is critically involved in controlling the accumulation of soluble sugars by coordinating carbohydrate synthesis and allocation,thus influencing sugar transport and its metabolism during the development of apple fruits.
文摘The WSC proteins produced by Penicillium expansum play a crucial role in causing blue mold on pears.To analyze the role of the WSC1 gene in the pathogenic process of this fungal pathogen,we conducted transcriptomic analysis of a WSC1 knockout(ΔWSC1)strain.The knockout of WSC1 significantly altered the gene expression profile in P.expansum,particularly for genes involved in cell wall integrity,signaling,stress response,and toxin production.The differential expression of these genes might make theΔWSC1 strain more vulnerable to environmental stress,while reducing the toxin production capacity,ultimately leading to a decrease in the pathogenicity.The transcriptomic analysis revealed that the expression of genes related to stress response signals,defense mechanisms and oxidative stress management changed when pear fruits were infected with theΔWSC1 strain.These changes may trigger a cascade of responses in pear fruits.In addition,compared with those infected with the wild-type strain,pear fruits infected with theΔWSC1 strain exhibited up-regulated expression of genes related to defense and oxidative stress.This study clarifies how the WSC1 gene influences P.expansum’s ability to infect pear fruits and how pear fruits respond to the infection.
基金Supported by Science and Technology Project of Guangdong Province(2007A020300002-12)~~
文摘According to the requirements of agricultural production and usem, taking diagnosis and decision-making of prevention for common diseases and pests in fruits and vegetables in southern China as the core, with communication and sharing as principle, adopted diagnosis, inquiries and guiding prevention of diseases and pests in fruits and vegetables as purpose, expert examination system of plant disease and pests in fruits and vegetables based on Web highly integrates the knowledge and prevention techniques of common diseases and pests for main fruit and vegetable in south China. In this system, the users can browse and inquiry the information about the fruit and vegetable diseases and pests, as well as their diagnosis and control. The implementation of the system plays an active role in promo- ting plant protection knowledge and guiding farms to scientifically control diseases and pests in fruits and vegetables
基金Supported by the Scientific and Technological Development Project of Shandong Province(2008GG2NS02022)the Special Fund for Agricultural Fine Seed Project in Shandong Province(2009LZ01-03)~~
文摘[Objective] This study aimed to compare and analyse the morphological characteristics of the seed-like fruits between Salvia shandongensis J. X. Li et F. Q. Zhou and its relatives. [Method] The morphological characteristics of the mature seed-like fruits of Salvia shandongensis and its three congeners, S. miltiorrhiza, S. miltiorrhiza f. alba and S. bowleyana, were comparatively observed using both optical microscope and scanning electron microscope. [Result] The results showed that there were distinct differences in the shape, size and exine sculpture of the seed-like fruits of the four Danshen congeners： the seed-like fruits of S. shandongensis that were elliptical and had areolate sculpture with subquadrangular convex meshes as well as densely filamentous transverse lines were easy to be distinguished from those of S. miltiorrhiza Bge., which were broadly elliptical and had reticulate sculpture with subquadrate concave meshes. The seed-like fruits of S. bowleyana were fusiform and their ektexine had reticulate sculpture with polygonal meshes, which were slightly convex at the center, which are obviously different from those of S. shandongensis. [Conclusion] This study first accumulates data of the seed-like fruits of S. shandongensis, which provides important bases for establishing its taxonomical status and studying its germplasm. The morphological characteristics and reticulate sculpture on ektexine of the seed-like fruits S. miltiorrhiza Bge. are consistent with the previous report. The results in this study not only provide references for uncovering the genetic relationships between S. shandongensis and S. miltiorrhiza Bge., as well as the interspecific classification and identification. In addition, this study also provides new scientific proofs for the identification of Danshen species and their introduction and cultivation, genetics and breeding.
基金Supported by The Forestry Science and Technology Innovation Funded Project of Hunan Forestry Bureau,No.XLKY202221。
文摘BACKGROUND Fruits of Gardenia jasminoides(G.jasminoides)have extremely high medicinal value.However,the quality and traits of the plants vary significantly based on their provenances.In addition,the behaviour of the known bioactive components,such as geniposide and crocin,has been the primary focus of the research on G.jasminoides.However,the identification of unknown bioactive components and their metabolomics remains underexplored.Therefore,analysing the metabolic differences between gardenias from different sources is essential to provide a comprehensive theoretical basis for the evaluation of G.jasminoides and germplasm resource identification.AIM To systematically evaluate the morphology,secondary metabolites,typical active ingredients,and antioxidant activity of wild G.jasminoides fruits.METHODS Gardenia fruits were collected from different provenances.Metabolites were identified via ultra-high-performance liquid chromatography–tandem mass spectrometry(UPLC-MS/MS).The metabolic differences were compared using hierarchical cluster analysis(HCA).The antioxidant capacity was evaluated using 2,2-diphenyl-1-picrylhydrazyl radical scavenging assays and ferric reducing antioxidant power assays,and its correlation with typical active ingredients was analysed.RESULTS A total of 444 and 240 metabolites were identified using UPLC-MS/MS in positive and negative ion modes,respectively.The HCA results of the flavonoids indicated that the higher content of flavonoids was in the fruits from Lukou.The differential analysis of metabolites in fruits from Shaoyang,Miluo and Lukou showed that the fruit from Miluo had the highest upregulated differential metabolites.CONCLUSION The metabolic characteristics of the Ningxiang and Xiangxi extracts were similar,while those of Lukou,Miluo and Shaoyang extracts differed significantly.
基金Supported by the National Natural Science Foundation of China(No.31060045,31260091)~~
文摘[Objective] This study aimed to identify red pigment of Panax notoginseng fruits and explore the correlation between pigment content and total saponins of the fruits. [Method] The red pigment of Panax notoginseng fruits was preliminarily identi- fied with specific color reactions and UV-vis spectra, and the contents of the pigment and total saponins were determined via spectrophotometry. [Result] The red hues of the fruits were contributed by anthocyanins and/or the anthocyanidins. The contents of anthocyanins and total saponins of the fruits both decreased along with thinning of the red hues. The content difference of the anthocyanins in fruits with different red hues reached extremely significant level, but that of total saponins just reached significant level. [Conclusion] The red pigment of P. notoginseng fruits is anthocyanins which are of extremely significant positive correlation with total saponins in contents.
文摘The increasing incidence of neurodegenerative diseases(NDs)and the constraints of existing treatment methods have spurred a keen interest in investigating alternative therapies.Medicinal plants,renowned for their long-standing use in traditional medicine,offer a hopeful avenue for discovering new neuroprotective agents.This study emphasizes the potential neuroprotective characteristics of edible fruit plants in Bangladesh,specifically focusing on their traditional folk medicine uses for neurological disorders.This study provides an in-depth overview of the different types of edible fruit trees in Bangladesh and their phytochemicals,including flavonoids,terpenoids,and phenolic acids.This work examines the scientific data supporting the neuroprotective properties of bioactive chemicals from plants.It further explores the mechanisms by which these compounds work to counteract oxidative stress,decrease inflammation,and stimulate neurogenesis.Moreover,the study investigates toxicological characteristics and bioactive components of some fruits,emphasizing the importance of further investigation to measure their safety profile comprehensively.This thorough study highlights the potential benefits of Bangladesh's edible fruit trees as a rich source of neuroprotective chemicals.It also shows that additional research might lead to novel approaches for improving brain functioning and preventing NDs.
基金supported by grants from the National Key Research and Development Program of China(2022YFF1100204)the National Natural Science Foundation of China(31972088,32001715)。
文摘Plant polyphenols are potential substitutes for clinicalα-glucosidase inhibitors.Our previous studies indicated that prodelphinidins from Chinese bayberry leaves(BLPs)have strongerα-glucosidase inhibitory activity than other common polyphenols,but they have no safe history of consumption.There is a reasonable prospect that prodelphinidins from Chinese bayberry fruits(BFPs)can improve postprandial hyperglycemia,though known active components are only myricetin and cyanidin-3-O-glucoside.Hence,the aim of this study was to analyze structure and hypoglycemic effect of BFPs of“Biqi”(BQPs)and“Dongkui”(DKPs)cultivars,and compare their difference with BLPs.The contents of BQPs and DKPs were(221.73±0.60)and(136.82±4.33)mg epicatechin(EC)equivalents/g dry weight,respectively.The most subunits were epigallocatechin gallate along with a small amount of epigallocatechin,epicatechin gallate and EC connected by B-type linkages and a small portion of A-type linkages with mean polymerization degree of 4.25 and 4.08,respectively.Importantly,BQPs and DKPs wereα-glucosidase inhibitors with half inhibitory concentration of 11.91 and 9.47μg/m L respectively,which were significantly stronger than BLPs.DKPs could also improve postprandial hyperglycemia of normal mice and high fat diet-induced type II diabetic mice.Therefore,edible prodelphinidins,which have stronger hypoglycemic effect than BLPs,were first found in Chinese bayberry fruits.
文摘The present study was carried out to make new healthy synbiotic flavored fermented skim milk drinks (SFFSD) supplemented with either Doum (Hyphaene thebaica L.) or Carob (Ceratonia siliqua) fruits powder for nutritional, antioxidant and antimicrobial activities. The HPLC analysis showed higher phenolic and flavonoid content in Doum extract than that of Carob. The antimicrobial activity of the methanolic extracts of both Doum and Carob against foodborne pathogens showed that the most sensitive indicators were Bacillus cereus, C. albicans and S. aureus, followed by T. mentagrophyte and E. coli wherein, the diameter of clear zones was, 29, 27, 24, 23 and 13 mm, respectively. While no effect was noticed against Aspergillus flavus. On the other hand, the methanolic extract of Carob fruit exhibited only a weak antibacterial effect against B. cereus (20 mm). Six treatments of SFFSD were made using both Doum or Carob fruit powder as prebiotic and Lactobacillus paracasei as probiotic bacteria. The added levels from Doum were 2%, 4% and 6%, while that added from Carob were 5%, 10% and 15%. Adding Doum and Carob powder increased the containing of minerals;Ca, K, Mg and iron compared with control. Both viscosity and viability of L. paracasei for the SFFSD were increased with increasing the added levels from either Doum or Carob, compared with the control. Samples of SFFSD containing Carob powder, show superior sensory for all parameters and total score points during storage period, especially at a level of 10%. It seems evident that samples supplemented with Doum powder were also acceptable.
基金Supported by Key Technologies R&D Program of Technology Bureau in Ningxia Hui Autonomous Region of China
文摘This paper aimed to explore the major meteorological disasters impacts on the growth of Jujube fruits.By analyzing statistic characters of meteorological data and historic yield data of Jujube fruits,the influence of different factors on the different growing stages of Jujube fruits was studied,in terms of temperature,precipitation,and relative humidity.The major meteorological disasters which impacted the growth of Jujube fruits were as follow:(1) Hot days when the temperature was equal to or above 30 ℃ continued over 9 days at full bloom stage;(2) The continuous rainy days lasted over 10 days at mature stage;(3) The continuous rainy days lasted over 7 days at full bloom stage;(4) Clod days when the temperature was equal to or below-22 ℃ continued over 4 days and the minimum temperature reached-24 ℃ at wintering stage.The major meteorological disasters which impact Jujube fruits were different at different stages.The most frequently occurred major meteorological disasters was the hot days when the temperature was equal to or above 30 ℃ and lasted over 9 days at full bloom stage.
基金supported by the National Key Research and Development Program(2016YFD0400101)the National Natural Science Foundation of China(31572175,31772370)+1 种基金the Fundamental Research Funds for the Central Universities(2018CDXYSM0021)the Committee of Science and Technology of Chongqing(cstckjcxljrc15).
文摘Genetic manipulation of genes to upregulate specific branches of metabolic pathways is a method that is commonly used to improve fruit quality.However,the use of a single gene to impact several metabolic pathways is difficult.Here,we show that overexpression of the single gene SlMYB75(SlMYB75-OE)is effective at improving multiple fruit quality traits.In these engineered fruits,the anthocyanin content reached 1.86mg g−1 fresh weight at the red-ripe stage,and these SlMYB75-OE tomatoes displayed a series of physiological changes,including delayed ripening and increased ethylene production.In addition to anthocyanin,the total contents of phenolics,flavonoids and soluble solids in SlMYB75-OE fruits were enhanced by 2.6,4,and 1.2 times,respectively,compared to those of wild-type(WT)fruits.Interestingly,a number of aroma volatiles,such as aldehyde,phenylpropanoid-derived and terpene volatiles,were significantly increased in SlMYB75-OE fruits,with some terpene volatiles showing more than 10 times higher levels than those in WT fruits.Consistent with the metabolic assessment,transcriptomic profiling indicated that the genes involved in the ethylene signaling,phenylpropanoid and isoprenoid pathways were greatly upregulated in SlMYB75-OE fruits.Yeast one-hybrid and transactivation assays revealed that SlMYB75 is able to directly bind to the MYBPLANT and MYBPZM cis-regulatory elements and to activate the promoters of the LOXC,AADC2 and TPS genes.The identification of SlMYB75 as a key regulator of fruit quality attributes through the transcriptional regulation of downstream genes involved in several metabolic pathways opens new avenues towards engineering fruits with a higher sensory and nutritional quality.
基金funded by the National Natural Science Fundation of China (30840016)the Natural Science Fundation of Jiangsu Province, China (BK 2010310)the Natural Science Fundation for Colleges and Universities in Jiangsu Province, China (10KJB550004)
文摘Peach fruits [Prumus persica （L.） Batsch, cv. Yuhuasanhao] were used as materials to investigate the changes of reactive oxygen species （ROS） and related enzymes in mitochondria respiration during storage and then their influence on senescence of harvested Peach fruits was studied. The results showed that low temperature （5℃） strongly inhibited the reduction of firmness and the increase in respiration rate. During storage at ambient temperature （20℃）, ROS had a cumulative process while malondialdehye （MDA） content continued to increase in associated with enhanced membrane lipid peroxidation. Lipoxygenase （LOX） activity was strongly inhibited under the low temperature condition. The activities of succinic dehydrogenase （SDH）, cytochrome C oxidase （CCO）, and Ca^2＋-ATPase declined to a certain extent at ambient temperature, while they showed higher activities at low temperature, which may be related to lower membrane lipid peroxidation at low temperature. Higher Ca^2＋ content at ambient temperature may be responsible for impairment of mitochondrial function, thus, leading to fruit senescence. The results showed that under low temperature condition, the low accumulation of ROS and the low level of membrane lipid peroxidation could maintain the function of mitochondria that would help to delay the senescence of peach fruits. These suggested a close relationship existed between ROS metabolism and mitochondrial respiration. It can be inferred that the low temperature helps to delay senescence of peach fruits via suppression of ROS and related enzymes, maintain better homeostasis of Ca^2＋ in mitochondria and thus better mitochondrial functions.
文摘Objective:To investigate the anti-inflammatory effects and the action mechanism of the fruits of Horenia dulcis(H.dulcis) in lipopolysaccharide(LPS)-induced mouse macrophage Raw 264.7cells.Methods:The extract of H.dulcis fruits(EHDF) were extracted with 70%ethanol.Mouse macrophages were treated with different concentrations of EHDF in the presence and absence of LPS(1 μg/mL).To demonstrate the inflammatory mediators including nitric oxide,inducible nitric oxide synthase and cyclooxygenase(COX)-2 expression levels were analyzed by usingin vitro assay systems.COX-derived pro-inflammatory cytokines including interleukin-1 β.tumor necrosis factor- α and prostaglandin F_2 were determined using ELISA kits.Cell viability,heme oxygenase-1 expression,nuclear factor-kappaB and nuclear factor F.2-related factors 2 translocation were also investigated.Results:EHDF potently inhibited the LPS-stimulated nitric oxide,inducible nitric oxide synthase.COX-2,interleukin-1 β and tumor necrosis factor- α expression in a dose-dependent manner.EHDF suppressed the phosphorylation of inhibited kappaB-alpha and p65 nuclear translocation.Treatment of macrophage cells with EHDF alone induced the heme oxygenase-1 and nuclear translocation of nuclear factor E2-reIated factor 2.Conclusions:These results suggest that the ethanol extract of H.dulcis fruit exerts its anti-inflammatory effects by inhibiting inhibited kappaBalpha phorylation and nuclear translocation of nuclear factor-kappaB.
基金financial support of the National Key Technology Research and Development Program(2012BAD29B01)the Key Basic Research Program(2015FY111200)of the Ministry of Science and Technology,China.
文摘In this paper,we report the construction of two accurate mass databases and the development of a combination detection method that simultaneously screens for 733 pesticide and chemical contaminant multi-residues via high-throughput liquid chromatography(LC)-and gas chromatography(GC)-quadru pole-time-of-flight mass spectrometry(Q-TOFMS).This work demonstrates that electronic mass spectral standards may replace chemical-source standard materials as references through one sample preparation and the combination of GC/LC-Q-TOFMS screening.This cutting-edge technique has also replaced multiresidue determination using targeted detection with non-targeted screening.The pesticide residue types,sensitivity,recovery,and reproducibility of this combination technique are evaluated in eight fruit and vegetable matrices.This technique shows three advantages:①In comparison with the discovery capability of a single technique,the combination technique shows an improvement of 51.1%(GC-QTOFMS)and 39.6%(LC-Q-TOFMS),respectively;②the combination technique can satisfy a screening limit lower than 10μg·kg^-1 and meet the requirements of“uniform standards,”although some of the pesticide residues could be optimized to further improve screening sensitivity;③over 488 pesticides with recoveries between 60%-120%and relative standard deviation(RSD)<20%at a spiked level of 10μg·kg^-1 were detected with the combination technique in eight different matrices.From 2012 to 2017,this combination technique was applied in an investigation to screen pesticide residues from 1384 sampling locations for 38138 batched samples covering 18 categories and 134 types of fruits and vegetables obtained from across the mainland of China.After statistical analysis,533 pesticides in 115891 determinations were detected,and the regularity of pesticides in the fruits and vegetables sold on the Chinese market was shown.
基金financially supported by the National Program for Quality and Safety Risk Assessment of Agricultural Products of China(GJFP2014002,GJFP2015002,and GJFP2016003)the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-ASTIP)the Core Research Budget of the Non-Profit Governmental Research Institution of China(0032014013)
文摘To assess levels of contamination and human health risk, we analyzed the concentrations of the heavy metals lead （Pb）, cadmium （Cd）, chromium （Cr）, and nickel （Ni） in China’s main deciduous fruits-apple, pear, peach, grape, and jujube. The concentration order of the heavy metals was Ni〉Cr〉Pb〉Cd. In 97.5% of the samples, heavy metal concentrations were within the maximum permissible limits. Among the fruits studied, the heavy metal concentrations in jujube and peach proved to be the highest, and those in grape proved to be the lowest. Only 2.2% of the samples were poluted by Ni, only 0.4% of the samples were poluted by Pb, and no samples were poluted by Cd or Cr. Compared with the other fruits, the combined heavy metal polution was signiifcantly higher （P〈0.05） in peach and signiifcantly lower （P〈0.05） in grape. For the combined heavy metal polution, 96.9% of the samples were at safe level, 2.32% at warning level, 0.65% at light level, and 0.13% at moderate level. In the fruits studied, the contribution of heavy metals to the daily intake rates （DIR） folowed the order of Ni〉Cr〉Pb〉Cd. The highest DIR came from apple, while the lowest DIR came from grape. For each of the heavy metals, the total DIR from ifve studied fruits corresponded to no more than 1.1% of the tolerable daily intake, indicating that no signiifcant adverse health effects are expected from the heavy metals and the fruits studied. The target hazard quotients and the total target hazard quotients demonstrated that none of the analyzed heavy metals may pose risk to consumers through the fruits studied. The highest risk was posed by apple, folowed in decreasing order by peach and pear, jujube, and grape. We suggest that the main deciduous fruits （apple, pear, peach, grape, and jujube） of China’s main producing areas are safe to eat.
基金the grants fromthe National Natural Science Foundation of China(NNSF-30170663) the Chinese Academy of Sciences.
文摘Rhizopus rot of peach fruits could be significantly suppressed by Pichia membranefaciens. Polyphenol oxidase (PPO), peroxidase (POD) and phenylalanine ammonium-lyase (PAL) activities induced by inoculation with P. membrane faciens or R. stolonifer were studied in postharvest peach fruits. The activities of PPO and PAL in peaches increased significantly after being inoculated with P. membrane faciens + R. stolonifer by 24 h, the activities maintained at a high level throughout the experiment. Under the condition of infected with R. stolonifer alone, activity of PPO and PAL could also increased, but the levels were lower than those treated with P. membrane faciens+ R. stolonifer. However, fruits inoculaed with P. membrane-faciens + R. stolonifer or R. stolonifer alone did not stimulated POD activity. The results suggest that the activation of these defense enzymes is involved in the action of P. membrane faciens against R. stolonifer.
基金supported by the National Program for Quality and Safety Risk Assessment of Agricultural Products of China (GJFP2016003 and GJFP2017003)the Scientific and Technological Innovation Project of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP)
文摘In this study, 15 mycotoxins were detected in 233 nut and dried fruit samples from China. The 15 mycotoxins included aflatoxins （AFs： AFB1, AFB2, AFG1 and AFG2）, trichothecene toxins （TCs： T-2, ZEA, ENA, ENA1, ENB, ENB1 and BEA）, Alternaria toxins （ATs： TEN, AOH and AME） and ochratoxin A （OTA）. The mycotoxins were detected in 47.6% of the samples and all 15 of the mycotoxins were found. Two samples were positive for AFB1 and exceeded the maximum tolerable levels allowed in China. The contamination levels of the mycotoxins found in nuts, dried jujubes, raisins, dried figs and dried Iongans were in the ranges of 0.1-462.7, 0.2-247.3, 0.8-10.1,0.2-384.1 and 0.1-89.2 μg kg^-1, respectively. Dried figs （80.0%） had the highest incidence of mycotoxins, followed by dried Iongans （60.0%）, dried jujubes （57.1%）, nuts （43.6%） and raisins （26.7%）. The estimated daily intake （EDI） values of each individual mycotoxin and all of the mycotoxins collectively were calculated by both the deterministic approach （DA） and the probability approach （PA）. For risk characterization, dietary exposure to TCs, ATs and OTA through consumption of nuts and dried fruits according to both approaches, showed no health risk to Chinese adults by exposure to either individual mycotoxins or in combination. To the best of our knowledge, this is the first work in which risk assessment of multimycotoxins is performed, specifically including the emerging ENNs and BEA, in nuts and dried fruits of China.
