Fructus corni is the dried sarcocarp of Cornus officinalis Sieb.et Zucc.It has been used as an important traditional Chinese medicine.Its action is to protect liver and kidney and regulate the essence of human body.In...Fructus corni is the dried sarcocarp of Cornus officinalis Sieb.et Zucc.It has been used as an important traditional Chinese medicine.Its action is to protect liver and kidney and regulate the essence of human body.In this paper,the research progress on the chemical ingredients and pharmacological activities of Fructus corni are reviewed.According to its pharmacological activities,some suggestions for future research and development have been made.展开更多
The components of crude and processed Fructus Corni were investigated by means of electrospray ionization-tandem mass spectrometry（ESI-MSn） technique in the negative ion mode. Compared with those of crude Fructus Co...The components of crude and processed Fructus Corni were investigated by means of electrospray ionization-tandem mass spectrometry（ESI-MSn） technique in the negative ion mode. Compared with those of crude Fructus Corni, the chemical components of the processed Fructus Corni were changed both in quality and in quantity. From the ESI-MS spectra of the crude and processed Fructus Corni, six peaks were selected to establish the characte-ristic ESI-MS peaks. Several factors in the processing procedure were examined. The experimental results demonstrate that the chemical reactions that occurred in the processing procedure can be used for the elucidation of the processed mechanism of Fructus Corni, which is regularly affected by the processing conditions. The present article provides both the chemistry evidence for the understanding of the processing procedure of Fructus Corni and the specific methodology for the research of the processing procedure and quality identification of traditional Chinese medicine.展开更多
As one of the most common metabolic diseases,type 2 diabetes(T2D)has become a public health concern with rising global prevalence.Corni fructus(CF),a traditional Chinese herb utilized for centuries as homologous medic...As one of the most common metabolic diseases,type 2 diabetes(T2D)has become a public health concern with rising global prevalence.Corni fructus(CF),a traditional Chinese herb utilized for centuries as homologous medicinal and food resources,has been widely used to treat glucose and lipid metabolism disorders.However,as a core active ingredient of CF,whether and how morroniside(MOR)improves T2D is still unclear.This study aimed to explore the pathways by which MOR ameliorates T2D in mice induced by high-fat diet(HFD)and low-dose streptozotocin through 16S rRNA gene sequencing.We found that MOR treatment significantly ameliorated body weight loss,hyperglycemia,hyperlipidemia and insulin resistance in T2D mice.In addition,MOR remarkably improved inflammation-and oxidative stress-driven hepatic and pancreatic injuries in the model mice.Mechanistically,MOR rehabilitated the dysregulated diversity and constitution of the gut microbiota in T2D mice,with significant changes in relative abundance in genus such as Lactobacillus,Lachnospiraceae_NK4A136_group,and Lachnospiraceae_UCG-006 which are believed to be highly correlated with serum parameters and insulin resistance in mice with T2D.Therefore,we infer that MOR improves T2D at least partially by maintaining the host microbiota homeostasis,and MOR may be a promising candidate for T2D treatment.展开更多
Zhang Xichun classifed the collapse syndrome into upper collapse,lower collapse and exterior col-lapse.Based on the therapeutic principle of tonifying the liver and astringing the liver to consolidate collapse,he used...Zhang Xichun classifed the collapse syndrome into upper collapse,lower collapse and exterior col-lapse.Based on the therapeutic principle of tonifying the liver and astringing the liver to consolidate collapse,he used drugs boldly to cure disease.The method of tonifying the liver and astringing sweating is applied to res-cue exterior collapse with Laifu Deoction(来复汤);the method of tonifying the liver and relieving asthma is applied to treat upper collapse with Shenzhe Zhenqi Decoction(参赭镇气汤);the method of restoring yang with emergent treatment is applied to rescue the lower collapse with Jijiu Huiyang Decoction(急救回阳汤).This will provide references for the prevention and treatment of critical diseases for later generations in practi-cing Chinese medicine.展开更多
Corni fructus(CF),the fruit of Cornus officinalis,is widely used in traditional Chinese medicine.This study investigated the effects of CF extract and Atorvastatin(AS)on obesity-related disorders.Twenty-five mice were...Corni fructus(CF),the fruit of Cornus officinalis,is widely used in traditional Chinese medicine.This study investigated the effects of CF extract and Atorvastatin(AS)on obesity-related disorders.Twenty-five mice were initially divided into two groups:a normal diet and a high-fat diet(HFD)group.After developing obesity,the HFD group was split into four subgroups:continued HFD,HFD with CF(400 mg/kg BW),HFD with CF(400 mg/kg BW)and AS(3 mg/kg BW),and HFD with AS(3 mg/kg BW).After six weeks of treatment,biochemical parameters were measured in each group(n=5).The mRNA expression of tumor necrosis factor-alpha(TNF-α),peroxisome proliferator-activated receptor gamma(PPAR-γ),monocyte chemoattractant protein-1(MCP-1),and glucose transporter 4(GLUT-4)was also analyzed.The molecular docking of the fruit-derived compounds was also performed with TNFα,PPAR-γ,MCP-1,GLUT4,and HMG-CoA reductase.The individual administration of CF and AS in obese mice significantly(p<0.05)reduced body weight,abdominal fat,liver weight,triglyceride,and cholesterol levels.Co-administration of CF and AS resulted in more pronounced effects.Most importantly,CF treatment significantly lowered triglyceride and low-density lipoprotein levels,while significantly increasing high-density lipoprotein levels in HFD-treated mice(p<0.05).Furthermore,the CF extract significantly(p<0.05)reduced aspartate aminotransferase and alkaline phosphatase levels in AS+HFD-treated mice.Consistent with these biochemical findings,a notable decrease in the mRNA expression of the studied genes was observed.These findings suggested that the combined use of CF with AS may substantially reduce obesity-related metabolic disorders.展开更多
The protective effect of 5-hydroxymethylfurfural（5-HMF） against hydrogen peroxide（H2O2）-induced injured in human umbilical vein endothelial cells（HUVECs） was examined. Treatment of HUVECs with 5-HMF significantl...The protective effect of 5-hydroxymethylfurfural（5-HMF） against hydrogen peroxide（H2O2）-induced injured in human umbilical vein endothelial cells（HUVECs） was examined. Treatment of HUVECs with 5-HMF significantly increased their viability, decreased cell apoptosis induced by H2O2, accompanied by the decreased nitric oxide level. Western blotting analysis demonstrated that Bcl-2 expression was increased whereas Caspase-3 and NF-κB expression was decreased in 5-HMF groups. In conclusion, the results showed that 5-HMF could prevent endothelial dysfunction and related complications caused by oxidative stress.展开更多
Background:To explore the action mechanism of Huangqi(Radix Astragali)and Shanzhuyu(Fructus Corni)in the treatment of diabetic nephropathy based on network pharmacology,aiming to provide a basis for clinical applicati...Background:To explore the action mechanism of Huangqi(Radix Astragali)and Shanzhuyu(Fructus Corni)in the treatment of diabetic nephropathy based on network pharmacology,aiming to provide a basis for clinical application.Methods:The main active components of Huangqi and Shanzhuyu were discerned through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),Traditional Chinese Medicine Integrated Database(TCMID).The targets related to diabetic nephropathy(DN)were obtained using Genecards,Therapeutic Target Database(TTD),and National Center for Biotechnology Information(NCBI)Gene.Subsequently,protein-protein interaction(PPI)networks were constructed with Cytoscape 3.7.2 and the STRING database.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses were performed to analyze the intersection of targets via Database for Annotation,Visualization,and Integrated Discovery(DAVID)6.8.Built on the above analysis,we made a“Chinese medicine-chemical composition-target gene-disease”network.Results:Twenty-one active components were predicted from the Huangqi and Shanzhuyu herb pair,such as jaranol,mandenol and sitosterol.These components were applied to 41 targets mainly involved in many biological processes such as the PI3K-Akt signaling pathway,vascular endothelial growth factor(VEGF)signaling pathway,regulation of sodium ion transport and steroid-binding.Conclusion:This study proposes the network pharmacology method and identifies the potent combination therapeutic mechanism of Huangqi and Shanzhuyu for diabetic nephropathy(DN)through multiple targets and routes,this strategy will lay a good foundation for further in-depth study of the mechanism of action.展开更多
Corni Fructus(CF)has great potential as a food product in the treatment of T2DM(thirst-quenching).As the main active ingredient of CF,loganin(LOG)possesses multiple pharmacological propertiesincluding hypoglycemic,hyp...Corni Fructus(CF)has great potential as a food product in the treatment of T2DM(thirst-quenching).As the main active ingredient of CF,loganin(LOG)possesses multiple pharmacological propertiesincluding hypoglycemic,hypolipidemic,anti-inflammatory and antibacterial.In this study,we firstly verified the efficacy of LOG in improving the disordered glycolipid metabolism and liver damages in a murine insulin resistance(IR)model,and then determined the ameliorative effect of LOG on IR-HepG2 cell model.To clarify the mechanism of LOG on IR,a series of differentially expressed metabolites(DEM)and genes(DEG)were screened out through non-targeted metabolomics and transcriptomics techniques.In addition,integrative metabolomics and transcriptomics analyses revealed a few key pathways(foxO signaling pathway,glycine serine and threonine metabolism,glutamatergic synapse,cocaine addiction,amphetamine addiction,and valine leucine and isoleucine biosynthesis),important DEM(L-Glutamate),and DEGs(Sgk2,Grin3b,Acadsb,Grik1)were reversely regulated by LOG as compared with the IR model.It is hypothesized that LOG may exert the anti-IR effect via finely tuning amino acid metabolism.Taken together,our data may shine light on the screening of new intervention targets and developing of new LOG-related agents for IR treatment.展开更多
Conventional medicine-based Chinese herbal prescriptions,have fascinated much attention due to their extensive and unique diversity of biological effects without toxicity and/or adverse effects.Treatment with Hachimi-...Conventional medicine-based Chinese herbal prescriptions,have fascinated much attention due to their extensive and unique diversity of biological effects without toxicity and/or adverse effects.Treatment with Hachimi-jio-gan(Ba-Wei-Di-Huang-Wan in Chinese)improved the dysregulated levels of hyperglycemic condition-related oxidative stress generation,advanced glycation endproduct generation,and renal function parameters.These results indicate that Hachimi-jio-gan is a prospective therapeutic agent against the pathogenesis of diabetic nephropathy.Cornel iridoid glycosides and polyphenol are the active compounds of Corni Fructus,the active component of Hachimi-jio-gan,against kidney damage caused by diabetes.Additionally,major components of the Corni Fructus,morroniside and 7-O-Galloyl-D-sedoheptulose(GS)are considered to be important contributors to prevent and/or delay the onset of kidney damage caused by diabetes.Chief of all,GS is expected to be developed as a novel therapeutic drug for the diabetes-accelerated kidney damage.展开更多
基金National Natural Science Foundation of China (Grant No.30873438/c190801).
文摘Fructus corni is the dried sarcocarp of Cornus officinalis Sieb.et Zucc.It has been used as an important traditional Chinese medicine.Its action is to protect liver and kidney and regulate the essence of human body.In this paper,the research progress on the chemical ingredients and pharmacological activities of Fructus corni are reviewed.According to its pharmacological activities,some suggestions for future research and development have been made.
基金the National Natural Science Foundation of China(No.30672600)the Great Research Project of Chinese Academy of Sciences(No.KGCX2-SW-213-06)+1 种基金the Natural Science Foundation of Jilin Province(No.20060902)the Natural Science Foundation of Changchun City, China(No.04-02GG233)
文摘The components of crude and processed Fructus Corni were investigated by means of electrospray ionization-tandem mass spectrometry（ESI-MSn） technique in the negative ion mode. Compared with those of crude Fructus Corni, the chemical components of the processed Fructus Corni were changed both in quality and in quantity. From the ESI-MS spectra of the crude and processed Fructus Corni, six peaks were selected to establish the characte-ristic ESI-MS peaks. Several factors in the processing procedure were examined. The experimental results demonstrate that the chemical reactions that occurred in the processing procedure can be used for the elucidation of the processed mechanism of Fructus Corni, which is regularly affected by the processing conditions. The present article provides both the chemistry evidence for the understanding of the processing procedure of Fructus Corni and the specific methodology for the research of the processing procedure and quality identification of traditional Chinese medicine.
基金supported by the Administration of Traditional Chinese Medicine Special Fund of Shaanxi Province(2021-QYZL-01,2021-QYZL-03)Special Funding for Basic Research Operating Expenses of the Central Universities(GK202205001,GK202205010)Hebei Province Innovation Capacity Enhancement Program Project(225A2501D).
文摘As one of the most common metabolic diseases,type 2 diabetes(T2D)has become a public health concern with rising global prevalence.Corni fructus(CF),a traditional Chinese herb utilized for centuries as homologous medicinal and food resources,has been widely used to treat glucose and lipid metabolism disorders.However,as a core active ingredient of CF,whether and how morroniside(MOR)improves T2D is still unclear.This study aimed to explore the pathways by which MOR ameliorates T2D in mice induced by high-fat diet(HFD)and low-dose streptozotocin through 16S rRNA gene sequencing.We found that MOR treatment significantly ameliorated body weight loss,hyperglycemia,hyperlipidemia and insulin resistance in T2D mice.In addition,MOR remarkably improved inflammation-and oxidative stress-driven hepatic and pancreatic injuries in the model mice.Mechanistically,MOR rehabilitated the dysregulated diversity and constitution of the gut microbiota in T2D mice,with significant changes in relative abundance in genus such as Lactobacillus,Lachnospiraceae_NK4A136_group,and Lachnospiraceae_UCG-006 which are believed to be highly correlated with serum parameters and insulin resistance in mice with T2D.Therefore,we infer that MOR improves T2D at least partially by maintaining the host microbiota homeostasis,and MOR may be a promising candidate for T2D treatment.
基金We thank for the funding support from the Scientific Research Project of Hunan Provincial Department of Education(18C0378)。
文摘Zhang Xichun classifed the collapse syndrome into upper collapse,lower collapse and exterior col-lapse.Based on the therapeutic principle of tonifying the liver and astringing the liver to consolidate collapse,he used drugs boldly to cure disease.The method of tonifying the liver and astringing sweating is applied to res-cue exterior collapse with Laifu Deoction(来复汤);the method of tonifying the liver and relieving asthma is applied to treat upper collapse with Shenzhe Zhenqi Decoction(参赭镇气汤);the method of restoring yang with emergent treatment is applied to rescue the lower collapse with Jijiu Huiyang Decoction(急救回阳汤).This will provide references for the prevention and treatment of critical diseases for later generations in practi-cing Chinese medicine.
基金funded by the Research Cell of Noakhali Science and Technology University,Bangladesh.
文摘Corni fructus(CF),the fruit of Cornus officinalis,is widely used in traditional Chinese medicine.This study investigated the effects of CF extract and Atorvastatin(AS)on obesity-related disorders.Twenty-five mice were initially divided into two groups:a normal diet and a high-fat diet(HFD)group.After developing obesity,the HFD group was split into four subgroups:continued HFD,HFD with CF(400 mg/kg BW),HFD with CF(400 mg/kg BW)and AS(3 mg/kg BW),and HFD with AS(3 mg/kg BW).After six weeks of treatment,biochemical parameters were measured in each group(n=5).The mRNA expression of tumor necrosis factor-alpha(TNF-α),peroxisome proliferator-activated receptor gamma(PPAR-γ),monocyte chemoattractant protein-1(MCP-1),and glucose transporter 4(GLUT-4)was also analyzed.The molecular docking of the fruit-derived compounds was also performed with TNFα,PPAR-γ,MCP-1,GLUT4,and HMG-CoA reductase.The individual administration of CF and AS in obese mice significantly(p<0.05)reduced body weight,abdominal fat,liver weight,triglyceride,and cholesterol levels.Co-administration of CF and AS resulted in more pronounced effects.Most importantly,CF treatment significantly lowered triglyceride and low-density lipoprotein levels,while significantly increasing high-density lipoprotein levels in HFD-treated mice(p<0.05).Furthermore,the CF extract significantly(p<0.05)reduced aspartate aminotransferase and alkaline phosphatase levels in AS+HFD-treated mice.Consistent with these biochemical findings,a notable decrease in the mRNA expression of the studied genes was observed.These findings suggested that the combined use of CF with AS may substantially reduce obesity-related metabolic disorders.
基金The Chinese Medicine Research Program of Zhejiang Province,China(Grant No.2014ZQ008)the Science and Technology Project of Hangzhou,China(Grant No.20130533B68,and 20131813A23)+1 种基金the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD,Grant No.2011ZYX2-006)the Medical Scientific Research Foundation of Zhejiang Province,China(Grant No.2013KYB183)
文摘The protective effect of 5-hydroxymethylfurfural（5-HMF） against hydrogen peroxide（H2O2）-induced injured in human umbilical vein endothelial cells（HUVECs） was examined. Treatment of HUVECs with 5-HMF significantly increased their viability, decreased cell apoptosis induced by H2O2, accompanied by the decreased nitric oxide level. Western blotting analysis demonstrated that Bcl-2 expression was increased whereas Caspase-3 and NF-κB expression was decreased in 5-HMF groups. In conclusion, the results showed that 5-HMF could prevent endothelial dysfunction and related complications caused by oxidative stress.
文摘Background:To explore the action mechanism of Huangqi(Radix Astragali)and Shanzhuyu(Fructus Corni)in the treatment of diabetic nephropathy based on network pharmacology,aiming to provide a basis for clinical application.Methods:The main active components of Huangqi and Shanzhuyu were discerned through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),Traditional Chinese Medicine Integrated Database(TCMID).The targets related to diabetic nephropathy(DN)were obtained using Genecards,Therapeutic Target Database(TTD),and National Center for Biotechnology Information(NCBI)Gene.Subsequently,protein-protein interaction(PPI)networks were constructed with Cytoscape 3.7.2 and the STRING database.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses were performed to analyze the intersection of targets via Database for Annotation,Visualization,and Integrated Discovery(DAVID)6.8.Built on the above analysis,we made a“Chinese medicine-chemical composition-target gene-disease”network.Results:Twenty-one active components were predicted from the Huangqi and Shanzhuyu herb pair,such as jaranol,mandenol and sitosterol.These components were applied to 41 targets mainly involved in many biological processes such as the PI3K-Akt signaling pathway,vascular endothelial growth factor(VEGF)signaling pathway,regulation of sodium ion transport and steroid-binding.Conclusion:This study proposes the network pharmacology method and identifies the potent combination therapeutic mechanism of Huangqi and Shanzhuyu for diabetic nephropathy(DN)through multiple targets and routes,this strategy will lay a good foundation for further in-depth study of the mechanism of action.
基金supported by the Administration of Traditional Chinese Medicine Special Fund of Shaanxi Province(2021-QYZL-01,2021-QYZL-03)Special Funding for Basic Research Operating Expenses of the Central Universities(GK202205001,GK202205010)Hebei Province Innovation Capacity Enhancement Program Project(225A2501D).
文摘Corni Fructus(CF)has great potential as a food product in the treatment of T2DM(thirst-quenching).As the main active ingredient of CF,loganin(LOG)possesses multiple pharmacological propertiesincluding hypoglycemic,hypolipidemic,anti-inflammatory and antibacterial.In this study,we firstly verified the efficacy of LOG in improving the disordered glycolipid metabolism and liver damages in a murine insulin resistance(IR)model,and then determined the ameliorative effect of LOG on IR-HepG2 cell model.To clarify the mechanism of LOG on IR,a series of differentially expressed metabolites(DEM)and genes(DEG)were screened out through non-targeted metabolomics and transcriptomics techniques.In addition,integrative metabolomics and transcriptomics analyses revealed a few key pathways(foxO signaling pathway,glycine serine and threonine metabolism,glutamatergic synapse,cocaine addiction,amphetamine addiction,and valine leucine and isoleucine biosynthesis),important DEM(L-Glutamate),and DEGs(Sgk2,Grin3b,Acadsb,Grik1)were reversely regulated by LOG as compared with the IR model.It is hypothesized that LOG may exert the anti-IR effect via finely tuning amino acid metabolism.Taken together,our data may shine light on the screening of new intervention targets and developing of new LOG-related agents for IR treatment.
文摘Conventional medicine-based Chinese herbal prescriptions,have fascinated much attention due to their extensive and unique diversity of biological effects without toxicity and/or adverse effects.Treatment with Hachimi-jio-gan(Ba-Wei-Di-Huang-Wan in Chinese)improved the dysregulated levels of hyperglycemic condition-related oxidative stress generation,advanced glycation endproduct generation,and renal function parameters.These results indicate that Hachimi-jio-gan is a prospective therapeutic agent against the pathogenesis of diabetic nephropathy.Cornel iridoid glycosides and polyphenol are the active compounds of Corni Fructus,the active component of Hachimi-jio-gan,against kidney damage caused by diabetes.Additionally,major components of the Corni Fructus,morroniside and 7-O-Galloyl-D-sedoheptulose(GS)are considered to be important contributors to prevent and/or delay the onset of kidney damage caused by diabetes.Chief of all,GS is expected to be developed as a novel therapeutic drug for the diabetes-accelerated kidney damage.
