Through several years seedling-growing experiments in nursery in Daxing‘anMountains forest region,the author posed that the seedling cultivated for the region mustpossess the characteristics of being suited to thin a...Through several years seedling-growing experiments in nursery in Daxing‘anMountains forest region,the author posed that the seedling cultivated for the region mustpossess the characteristics of being suited to thin and meager soil and having strongresistance to adverse conditions.For this purpose,the cultivation regime ought to bedirectization,standardization and programmatization;seedling growth ought to be promotedby regulating ecological conditions;and the mursery stock quality ought to be tested usingvigor index.展开更多
Suihua City in Heilongjiang Province is located in the heart of the frigid region with black earth and it has created abundant green property resources. However,the environmental issue of this place has caused wide at...Suihua City in Heilongjiang Province is located in the heart of the frigid region with black earth and it has created abundant green property resources. However,the environmental issue of this place has caused wide attention,so the government should make full use of various means to solve the environment problem and protect the ecology in this region. Among the measures taken by the government,the tax means is the most effective economic device to solve the environmental problem. Economic means supplemented by lawful and administrative measures should be adopted to solve the ecological environmental problems in the frigid region with black earth.展开更多
Objective:Effective Chronic Kidney Disease(CKD)management is particularly important in cold regions of China,where climate and lifestyle factors play significant roles.However,there is a lack of relevant studies in th...Objective:Effective Chronic Kidney Disease(CKD)management is particularly important in cold regions of China,where climate and lifestyle factors play significant roles.However,there is a lack of relevant studies in this area.Therefore,the purpose of this study was:(1)to assess the status of self-management capacity in individuals with CKD in cold regions of China and analyze the factors influencing it;(2)to identify strategies to improve CKD management in primary care settings in these regions;and(3)to understand patients'attitudes toward eHealth services.Methods:This was a regional,cross-sectional observational study.A questionnaire measuring CKD patients'self-management abilities was derived from the Perceived Kidney Disease Self-Management Scale,the Kidney Disease Behavioral Inventory(KDBI),and the Health Literacy Questionnaire.Data were collected from hospitalized CKD patients in Heilongjiang Province and analyzed using One-Way Analysis of Variance(ANOVA),Hierarchical Regression Analysis,and K-prototype cluster analysis.Results:A total of 957 participants were tested.Of these,70.64%had less than a bachelor's degree,and 56.27%had been diagnosed with hypertensive or diabetic nephropathy.The KDBI scale showed a lower overall score compared to the PKDSMS.Factors such as CKD stage 4(F=2.367,P=0.042),last year's medical expenses(F=3.974,P=0.004),and poor self-rated health(F=33.352,P<0.01)were found to influence scores on both scales.The health literacy questionnaire revealed significant differences(P<0.01)in health knowledge,except by sex.Additionally,healthcare expenditures and poor self-rated health were negatively associated with self-management capacity.Conclusion:This study provides valuable insights into the self-management challenges faced by CKD patients in cold regions of China.Despite some difficulties in improving self-management,patients showed positive attitudes toward enhancing CKD management services in primary care and developing digital management tools.These findings offer useful references and recommendations for future clinical practice and research in this field.展开更多
目的:基于网络药理学和分子对接技术探究四逆失笑散加味方治疗子宫腺肌病(adenomyosis,AM)的作用机制。方法:利用中药系统药理学数据库和分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,T...目的:基于网络药理学和分子对接技术探究四逆失笑散加味方治疗子宫腺肌病(adenomyosis,AM)的作用机制。方法:利用中药系统药理学数据库和分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)筛选四逆失笑散加味方的活性成分,通过SwissTargetPrediction数据库和PharmMapper数据库预测化学成分的潜在作用靶点。通过DisGeNET与GeneCards数据库获取AM相关靶点。取药物与疾病的交集靶点并绘制Venn图。使用Cytoscape 3.10.0软件构建药物-成分-靶点网络,并进行拓扑分析。借助STRING数据库构建蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络,筛选核心靶点。使用DAVID数据库进行基因本体(Gene Ontology,GO)和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)信号通路富集分析。利用AutoDockTools 1.5.7软件对关键活性成分与核心靶点进行分子对接。结果:筛选得到四逆失笑散加味方有效活性成分257个,相关靶点1255个。AM的相关靶点390个,与四逆失笑散加味方有效活性成分相关靶点的交集95个。PPI网络分析显示,蛋白激酶B1(protein kinase B1,PKB1,又称AKT1)、白细胞介素-6(interleukin-6,IL-6)、表皮生长因子受体(epidermal growth factor receptor,EGFR)、TP53、肿瘤坏死因子(tumor necrosis factor,TNF)等可能为该方治疗AM的关键靶点。GO功能分析获得生物过程590条、细胞成分59条、分子功能102条,KEGG分析筛选出158条相关信号通路,其中磷脂酰肌醇3激酶(phosphoinositide 3-kinase,PI3K)-AKT信号通路、缺氧诱导因子-1(hypoxia-inducible factor-1,HIF-1)信号通路、IL-17信号通路及TNF信号通路等与AM的治疗密切相关。四逆失笑散加味方的关键活性成分quercetin(槲皮素)、kaempferol(山柰酚)、isorhamnetin(异鼠李素)、beta-sitosterol(β-谷甾醇)、sitosterol(谷甾醇)与核心靶点AKT1、IL-6、EGFR、TP53、TNF对接良好。结论:四逆失笑散加味方通过多成分、多靶点及多条信号通路协同作用治疗AM,其疗效可能与抗炎及抑制异位细胞增殖等过程密切相关。展开更多
文摘Through several years seedling-growing experiments in nursery in Daxing‘anMountains forest region,the author posed that the seedling cultivated for the region mustpossess the characteristics of being suited to thin and meager soil and having strongresistance to adverse conditions.For this purpose,the cultivation regime ought to bedirectization,standardization and programmatization;seedling growth ought to be promotedby regulating ecological conditions;and the mursery stock quality ought to be tested usingvigor index.
基金Supported by the Foundation for Outstanding Youth of Suihua University(SJ09005)
文摘Suihua City in Heilongjiang Province is located in the heart of the frigid region with black earth and it has created abundant green property resources. However,the environmental issue of this place has caused wide attention,so the government should make full use of various means to solve the environment problem and protect the ecology in this region. Among the measures taken by the government,the tax means is the most effective economic device to solve the environmental problem. Economic means supplemented by lawful and administrative measures should be adopted to solve the ecological environmental problems in the frigid region with black earth.
基金supported by the Natural Science Foundation of China(7247042122)the Key Research and Development Project of Heilongjiang Province,China(2024ZX12C06).
文摘Objective:Effective Chronic Kidney Disease(CKD)management is particularly important in cold regions of China,where climate and lifestyle factors play significant roles.However,there is a lack of relevant studies in this area.Therefore,the purpose of this study was:(1)to assess the status of self-management capacity in individuals with CKD in cold regions of China and analyze the factors influencing it;(2)to identify strategies to improve CKD management in primary care settings in these regions;and(3)to understand patients'attitudes toward eHealth services.Methods:This was a regional,cross-sectional observational study.A questionnaire measuring CKD patients'self-management abilities was derived from the Perceived Kidney Disease Self-Management Scale,the Kidney Disease Behavioral Inventory(KDBI),and the Health Literacy Questionnaire.Data were collected from hospitalized CKD patients in Heilongjiang Province and analyzed using One-Way Analysis of Variance(ANOVA),Hierarchical Regression Analysis,and K-prototype cluster analysis.Results:A total of 957 participants were tested.Of these,70.64%had less than a bachelor's degree,and 56.27%had been diagnosed with hypertensive or diabetic nephropathy.The KDBI scale showed a lower overall score compared to the PKDSMS.Factors such as CKD stage 4(F=2.367,P=0.042),last year's medical expenses(F=3.974,P=0.004),and poor self-rated health(F=33.352,P<0.01)were found to influence scores on both scales.The health literacy questionnaire revealed significant differences(P<0.01)in health knowledge,except by sex.Additionally,healthcare expenditures and poor self-rated health were negatively associated with self-management capacity.Conclusion:This study provides valuable insights into the self-management challenges faced by CKD patients in cold regions of China.Despite some difficulties in improving self-management,patients showed positive attitudes toward enhancing CKD management services in primary care and developing digital management tools.These findings offer useful references and recommendations for future clinical practice and research in this field.
文摘目的:基于网络药理学和分子对接技术探究四逆失笑散加味方治疗子宫腺肌病(adenomyosis,AM)的作用机制。方法:利用中药系统药理学数据库和分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)筛选四逆失笑散加味方的活性成分,通过SwissTargetPrediction数据库和PharmMapper数据库预测化学成分的潜在作用靶点。通过DisGeNET与GeneCards数据库获取AM相关靶点。取药物与疾病的交集靶点并绘制Venn图。使用Cytoscape 3.10.0软件构建药物-成分-靶点网络,并进行拓扑分析。借助STRING数据库构建蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络,筛选核心靶点。使用DAVID数据库进行基因本体(Gene Ontology,GO)和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)信号通路富集分析。利用AutoDockTools 1.5.7软件对关键活性成分与核心靶点进行分子对接。结果:筛选得到四逆失笑散加味方有效活性成分257个,相关靶点1255个。AM的相关靶点390个,与四逆失笑散加味方有效活性成分相关靶点的交集95个。PPI网络分析显示,蛋白激酶B1(protein kinase B1,PKB1,又称AKT1)、白细胞介素-6(interleukin-6,IL-6)、表皮生长因子受体(epidermal growth factor receptor,EGFR)、TP53、肿瘤坏死因子(tumor necrosis factor,TNF)等可能为该方治疗AM的关键靶点。GO功能分析获得生物过程590条、细胞成分59条、分子功能102条,KEGG分析筛选出158条相关信号通路,其中磷脂酰肌醇3激酶(phosphoinositide 3-kinase,PI3K)-AKT信号通路、缺氧诱导因子-1(hypoxia-inducible factor-1,HIF-1)信号通路、IL-17信号通路及TNF信号通路等与AM的治疗密切相关。四逆失笑散加味方的关键活性成分quercetin(槲皮素)、kaempferol(山柰酚)、isorhamnetin(异鼠李素)、beta-sitosterol(β-谷甾醇)、sitosterol(谷甾醇)与核心靶点AKT1、IL-6、EGFR、TP53、TNF对接良好。结论:四逆失笑散加味方通过多成分、多靶点及多条信号通路协同作用治疗AM,其疗效可能与抗炎及抑制异位细胞增殖等过程密切相关。
文摘目的:基于网络药理学与分子对接研究当归四逆汤治疗稳定型心绞痛(stable angina pectoris,SAP)的作用机制。方法:使用SyMmap数据库和中药系统药理学数据库与分析平台检索当归四逆汤组方中药甘草、大枣、当归、桂枝、细辛、通草和白芍的化学成分及相关靶点;在GeneCards数据库获取SAP相关疾病靶点;取SAP疾病相关靶点和当归四逆汤组方中药活性成分相关靶点的交集,即为当归四逆汤治疗SAP的潜在靶点,并绘制韦恩图。利用STRING 11.5数据库对潜在靶点进行蛋白质相互作用(protein protein interaction,PPI)网络分析并筛选核心靶点。采用Cytoscape 3.10.1构建“中药-活性成分-潜在靶点”网络,并筛选关键成分。采用Matascape对潜在靶点进行基因本体(Gene Ontology,GO)富集分析和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)信号通路富集分析。利用PyMOL4.6.0对关键靶点和成分进行分子对接。结果:从当归四逆汤组方中药共筛选得到155种化学成分及1728个相关靶点;在GeneCards数据库获得SAP相关靶点1089个;取交集得到当归四逆汤治疗SAP的潜在靶点121个。PPI网络分析发现信号传导及转录激活蛋白3(signal transducer and activator of transcription 3,STAT3)、B细胞淋巴因子2(B cell lymphoma 2,BCL2)、蛋白激酶B1(protein kinase B1,PKB1,又称AKT1)等为核心靶点;“中药-活性成分-潜在靶点”网络分析筛选出槲皮素、山柰酚、β-谷甾醇等为关键成分。GO分析得到4688个生物过程,404个细胞组分和715个分子功能;KEGG分析得到磷脂酰肌醇3-激酶(phosphoinositide 3-kinase,PI3K)-AKT、缺氧诱导因子1(hypoxia-inducible factor 1,HIF1)、丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)等200条信号通路。分子对接显示活性成分与靶点对接良好。结论:当归四逆汤中的多个化学成分通过调控多个相关靶点参与多条信号通路发挥治疗SAP的作用。
