Objective:To analyze the risk of osteoporosis among middle-aged men in the cold regions of China(Heilongjiang Province)and provide theoretical support for the early identification of high-risk populations.Methods:Bone...Objective:To analyze the risk of osteoporosis among middle-aged men in the cold regions of China(Heilongjiang Province)and provide theoretical support for the early identification of high-risk populations.Methods:Bone mineral density(BMD)data were collected from male subjects aged 50-65 who met the inclusion criteria at the physical examination center of a hospital in Harbin between August to December 2022.General clinical data and dietary information were obtained through face-to-face interviews using a dietary questionnaire survey.Results:The prevalence of osteoporosis and osteopenia was 14.38%and 52.06%,respectively,while normal bone mass accounted for 33.56%.Significant differences were observed among groups in smoking habits,sunlight exposure,exercise levels,and dietary patterns at each bone mass level.The BMD of the lumbar spine,femoral neck,and hip showed a negative correlation with the Dietary Inflammatory Index(DII)score.Multivariate logistic regression analysis revealed that smoking and a diet high in oil and salt were positively associated with the risk of osteoporosis.A pro-inflammatory diet was also positively correlated with osteoporosis risk,with individuals in this group being 7.723 times more likely to develop osteoporosis compared to those in the anti-inflammatory diet group.Conclusion:The high prevalence of osteoporosis and osteopenia observed in this study highlighted that osteoporosis is a significant and pressing issue among middle-aged men.Smoking,limited sunlight exposure,reduced physical activity,diets high in oil and salt,and pro-inflammatory diets were identified as major risk factors for bone loss.These factors are closely linked to the geography,climate,and cultural practices of cold regions in China.Primary healthcare in this region should focus on the screening and prevention of osteoporosis in middle-aged men by promoting smoking cessation,increased sunlight exposure,adequate vitamin D supplementation,regular physical activity,and adherence to a healthy diet to maintain bone health.展开更多
Through several years seedling-growing experiments in nursery in Daxing‘anMountains forest region,the author posed that the seedling cultivated for the region mustpossess the characteristics of being suited to thin a...Through several years seedling-growing experiments in nursery in Daxing‘anMountains forest region,the author posed that the seedling cultivated for the region mustpossess the characteristics of being suited to thin and meager soil and having strongresistance to adverse conditions.For this purpose,the cultivation regime ought to bedirectization,standardization and programmatization;seedling growth ought to be promotedby regulating ecological conditions;and the mursery stock quality ought to be tested usingvigor index.展开更多
Suihua City in Heilongjiang Province is located in the heart of the frigid region with black earth and it has created abundant green property resources. However,the environmental issue of this place has caused wide at...Suihua City in Heilongjiang Province is located in the heart of the frigid region with black earth and it has created abundant green property resources. However,the environmental issue of this place has caused wide attention,so the government should make full use of various means to solve the environment problem and protect the ecology in this region. Among the measures taken by the government,the tax means is the most effective economic device to solve the environmental problem. Economic means supplemented by lawful and administrative measures should be adopted to solve the ecological environmental problems in the frigid region with black earth.展开更多
Understanding the genetic mechanism of cold adaptation in cashmere goats and dairy goats is very important to improve their production performance.The purpose of this study was to comprehensively analyze the genetic b...Understanding the genetic mechanism of cold adaptation in cashmere goats and dairy goats is very important to improve their production performance.The purpose of this study was to comprehensively analyze the genetic basis of goat adaptation to cold environments,clarify the impact of environmental factors on genome diversity,and lay the foundation for breeding goat breeds to adapt to climate change.A total of 240 dairy goats were subjected to genome resequencing,and the whole genome sequencing data of 57 individuals from 6 published breeds were incorporated.By integrating multiple approaches such as phylogenetic analysis,population structure analysis,gene flow and population history exploration,selection signal analysis,and genome-environment association analysis,an in-depth investigation was carried out.Phylogenetic analysis unraveled the genetic relationships and differentiation patterns among dairy goats and other goat breeds.Through signal analysis(θπ,FST,XP-CLR),we identified numerous candidate genes associated with cold adaptation in dairy goats(STRIP1,ALX3,HTR4,NTRK2,MRPL11,PELI3,DPP3,BBS1)and cashmere goats(MED12L,MARC2,MARC1,DSG3,C6H4orf22,CHD7,MYPN,KIAA0825,MITF).Genomeenvironment association(GEA)analysis confirmed the link between these genes and environmental factors.Moreover,a detailed analysis of the critical genes C6H4orf22 and STRIP1 demonstrated their significant roles in the geographical variations of cold adaptation and allele frequency differences among different breeds.This study contributes to understanding the genetic basis of cold adaptation,providing crucial theoretical support for precision breeding programs aimed at improving production performance in cold regions by leveraging adaptive alleles,thereby ensuring sustainable animal husbandry.展开更多
Objective:Effective Chronic Kidney Disease(CKD)management is particularly important in cold regions of China,where climate and lifestyle factors play significant roles.However,there is a lack of relevant studies in th...Objective:Effective Chronic Kidney Disease(CKD)management is particularly important in cold regions of China,where climate and lifestyle factors play significant roles.However,there is a lack of relevant studies in this area.Therefore,the purpose of this study was:(1)to assess the status of self-management capacity in individuals with CKD in cold regions of China and analyze the factors influencing it;(2)to identify strategies to improve CKD management in primary care settings in these regions;and(3)to understand patients'attitudes toward eHealth services.Methods:This was a regional,cross-sectional observational study.A questionnaire measuring CKD patients'self-management abilities was derived from the Perceived Kidney Disease Self-Management Scale,the Kidney Disease Behavioral Inventory(KDBI),and the Health Literacy Questionnaire.Data were collected from hospitalized CKD patients in Heilongjiang Province and analyzed using One-Way Analysis of Variance(ANOVA),Hierarchical Regression Analysis,and K-prototype cluster analysis.Results:A total of 957 participants were tested.Of these,70.64%had less than a bachelor's degree,and 56.27%had been diagnosed with hypertensive or diabetic nephropathy.The KDBI scale showed a lower overall score compared to the PKDSMS.Factors such as CKD stage 4(F=2.367,P=0.042),last year's medical expenses(F=3.974,P=0.004),and poor self-rated health(F=33.352,P<0.01)were found to influence scores on both scales.The health literacy questionnaire revealed significant differences(P<0.01)in health knowledge,except by sex.Additionally,healthcare expenditures and poor self-rated health were negatively associated with self-management capacity.Conclusion:This study provides valuable insights into the self-management challenges faced by CKD patients in cold regions of China.Despite some difficulties in improving self-management,patients showed positive attitudes toward enhancing CKD management services in primary care and developing digital management tools.These findings offer useful references and recommendations for future clinical practice and research in this field.展开更多
目的:基于网络药理学和分子对接技术探究四逆失笑散加味方治疗子宫腺肌病(adenomyosis,AM)的作用机制。方法:利用中药系统药理学数据库和分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,T...目的:基于网络药理学和分子对接技术探究四逆失笑散加味方治疗子宫腺肌病(adenomyosis,AM)的作用机制。方法:利用中药系统药理学数据库和分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)筛选四逆失笑散加味方的活性成分,通过SwissTargetPrediction数据库和PharmMapper数据库预测化学成分的潜在作用靶点。通过DisGeNET与GeneCards数据库获取AM相关靶点。取药物与疾病的交集靶点并绘制Venn图。使用Cytoscape 3.10.0软件构建药物-成分-靶点网络,并进行拓扑分析。借助STRING数据库构建蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络,筛选核心靶点。使用DAVID数据库进行基因本体(Gene Ontology,GO)和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)信号通路富集分析。利用AutoDockTools 1.5.7软件对关键活性成分与核心靶点进行分子对接。结果:筛选得到四逆失笑散加味方有效活性成分257个,相关靶点1255个。AM的相关靶点390个,与四逆失笑散加味方有效活性成分相关靶点的交集95个。PPI网络分析显示,蛋白激酶B1(protein kinase B1,PKB1,又称AKT1)、白细胞介素-6(interleukin-6,IL-6)、表皮生长因子受体(epidermal growth factor receptor,EGFR)、TP53、肿瘤坏死因子(tumor necrosis factor,TNF)等可能为该方治疗AM的关键靶点。GO功能分析获得生物过程590条、细胞成分59条、分子功能102条,KEGG分析筛选出158条相关信号通路,其中磷脂酰肌醇3激酶(phosphoinositide 3-kinase,PI3K)-AKT信号通路、缺氧诱导因子-1(hypoxia-inducible factor-1,HIF-1)信号通路、IL-17信号通路及TNF信号通路等与AM的治疗密切相关。四逆失笑散加味方的关键活性成分quercetin(槲皮素)、kaempferol(山柰酚)、isorhamnetin(异鼠李素)、beta-sitosterol(β-谷甾醇)、sitosterol(谷甾醇)与核心靶点AKT1、IL-6、EGFR、TP53、TNF对接良好。结论:四逆失笑散加味方通过多成分、多靶点及多条信号通路协同作用治疗AM,其疗效可能与抗炎及抑制异位细胞增殖等过程密切相关。展开更多
文摘Objective:To analyze the risk of osteoporosis among middle-aged men in the cold regions of China(Heilongjiang Province)and provide theoretical support for the early identification of high-risk populations.Methods:Bone mineral density(BMD)data were collected from male subjects aged 50-65 who met the inclusion criteria at the physical examination center of a hospital in Harbin between August to December 2022.General clinical data and dietary information were obtained through face-to-face interviews using a dietary questionnaire survey.Results:The prevalence of osteoporosis and osteopenia was 14.38%and 52.06%,respectively,while normal bone mass accounted for 33.56%.Significant differences were observed among groups in smoking habits,sunlight exposure,exercise levels,and dietary patterns at each bone mass level.The BMD of the lumbar spine,femoral neck,and hip showed a negative correlation with the Dietary Inflammatory Index(DII)score.Multivariate logistic regression analysis revealed that smoking and a diet high in oil and salt were positively associated with the risk of osteoporosis.A pro-inflammatory diet was also positively correlated with osteoporosis risk,with individuals in this group being 7.723 times more likely to develop osteoporosis compared to those in the anti-inflammatory diet group.Conclusion:The high prevalence of osteoporosis and osteopenia observed in this study highlighted that osteoporosis is a significant and pressing issue among middle-aged men.Smoking,limited sunlight exposure,reduced physical activity,diets high in oil and salt,and pro-inflammatory diets were identified as major risk factors for bone loss.These factors are closely linked to the geography,climate,and cultural practices of cold regions in China.Primary healthcare in this region should focus on the screening and prevention of osteoporosis in middle-aged men by promoting smoking cessation,increased sunlight exposure,adequate vitamin D supplementation,regular physical activity,and adherence to a healthy diet to maintain bone health.
文摘Through several years seedling-growing experiments in nursery in Daxing‘anMountains forest region,the author posed that the seedling cultivated for the region mustpossess the characteristics of being suited to thin and meager soil and having strongresistance to adverse conditions.For this purpose,the cultivation regime ought to bedirectization,standardization and programmatization;seedling growth ought to be promotedby regulating ecological conditions;and the mursery stock quality ought to be tested usingvigor index.
基金Supported by the Foundation for Outstanding Youth of Suihua University(SJ09005)
文摘Suihua City in Heilongjiang Province is located in the heart of the frigid region with black earth and it has created abundant green property resources. However,the environmental issue of this place has caused wide attention,so the government should make full use of various means to solve the environment problem and protect the ecology in this region. Among the measures taken by the government,the tax means is the most effective economic device to solve the environmental problem. Economic means supplemented by lawful and administrative measures should be adopted to solve the ecological environmental problems in the frigid region with black earth.
基金supported by the Genome Sequence Archiv in National Genomics Data Center,China National Center for Bioinformation/Beijing Institute of Genomics(CRA017705)supported the discovers could been obtained from the corresponding authors.
文摘Understanding the genetic mechanism of cold adaptation in cashmere goats and dairy goats is very important to improve their production performance.The purpose of this study was to comprehensively analyze the genetic basis of goat adaptation to cold environments,clarify the impact of environmental factors on genome diversity,and lay the foundation for breeding goat breeds to adapt to climate change.A total of 240 dairy goats were subjected to genome resequencing,and the whole genome sequencing data of 57 individuals from 6 published breeds were incorporated.By integrating multiple approaches such as phylogenetic analysis,population structure analysis,gene flow and population history exploration,selection signal analysis,and genome-environment association analysis,an in-depth investigation was carried out.Phylogenetic analysis unraveled the genetic relationships and differentiation patterns among dairy goats and other goat breeds.Through signal analysis(θπ,FST,XP-CLR),we identified numerous candidate genes associated with cold adaptation in dairy goats(STRIP1,ALX3,HTR4,NTRK2,MRPL11,PELI3,DPP3,BBS1)and cashmere goats(MED12L,MARC2,MARC1,DSG3,C6H4orf22,CHD7,MYPN,KIAA0825,MITF).Genomeenvironment association(GEA)analysis confirmed the link between these genes and environmental factors.Moreover,a detailed analysis of the critical genes C6H4orf22 and STRIP1 demonstrated their significant roles in the geographical variations of cold adaptation and allele frequency differences among different breeds.This study contributes to understanding the genetic basis of cold adaptation,providing crucial theoretical support for precision breeding programs aimed at improving production performance in cold regions by leveraging adaptive alleles,thereby ensuring sustainable animal husbandry.
基金supported by the Natural Science Foundation of China(7247042122)the Key Research and Development Project of Heilongjiang Province,China(2024ZX12C06).
文摘Objective:Effective Chronic Kidney Disease(CKD)management is particularly important in cold regions of China,where climate and lifestyle factors play significant roles.However,there is a lack of relevant studies in this area.Therefore,the purpose of this study was:(1)to assess the status of self-management capacity in individuals with CKD in cold regions of China and analyze the factors influencing it;(2)to identify strategies to improve CKD management in primary care settings in these regions;and(3)to understand patients'attitudes toward eHealth services.Methods:This was a regional,cross-sectional observational study.A questionnaire measuring CKD patients'self-management abilities was derived from the Perceived Kidney Disease Self-Management Scale,the Kidney Disease Behavioral Inventory(KDBI),and the Health Literacy Questionnaire.Data were collected from hospitalized CKD patients in Heilongjiang Province and analyzed using One-Way Analysis of Variance(ANOVA),Hierarchical Regression Analysis,and K-prototype cluster analysis.Results:A total of 957 participants were tested.Of these,70.64%had less than a bachelor's degree,and 56.27%had been diagnosed with hypertensive or diabetic nephropathy.The KDBI scale showed a lower overall score compared to the PKDSMS.Factors such as CKD stage 4(F=2.367,P=0.042),last year's medical expenses(F=3.974,P=0.004),and poor self-rated health(F=33.352,P<0.01)were found to influence scores on both scales.The health literacy questionnaire revealed significant differences(P<0.01)in health knowledge,except by sex.Additionally,healthcare expenditures and poor self-rated health were negatively associated with self-management capacity.Conclusion:This study provides valuable insights into the self-management challenges faced by CKD patients in cold regions of China.Despite some difficulties in improving self-management,patients showed positive attitudes toward enhancing CKD management services in primary care and developing digital management tools.These findings offer useful references and recommendations for future clinical practice and research in this field.
文摘目的:基于网络药理学和分子对接技术探究四逆失笑散加味方治疗子宫腺肌病(adenomyosis,AM)的作用机制。方法:利用中药系统药理学数据库和分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)筛选四逆失笑散加味方的活性成分,通过SwissTargetPrediction数据库和PharmMapper数据库预测化学成分的潜在作用靶点。通过DisGeNET与GeneCards数据库获取AM相关靶点。取药物与疾病的交集靶点并绘制Venn图。使用Cytoscape 3.10.0软件构建药物-成分-靶点网络,并进行拓扑分析。借助STRING数据库构建蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络,筛选核心靶点。使用DAVID数据库进行基因本体(Gene Ontology,GO)和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)信号通路富集分析。利用AutoDockTools 1.5.7软件对关键活性成分与核心靶点进行分子对接。结果:筛选得到四逆失笑散加味方有效活性成分257个,相关靶点1255个。AM的相关靶点390个,与四逆失笑散加味方有效活性成分相关靶点的交集95个。PPI网络分析显示,蛋白激酶B1(protein kinase B1,PKB1,又称AKT1)、白细胞介素-6(interleukin-6,IL-6)、表皮生长因子受体(epidermal growth factor receptor,EGFR)、TP53、肿瘤坏死因子(tumor necrosis factor,TNF)等可能为该方治疗AM的关键靶点。GO功能分析获得生物过程590条、细胞成分59条、分子功能102条,KEGG分析筛选出158条相关信号通路,其中磷脂酰肌醇3激酶(phosphoinositide 3-kinase,PI3K)-AKT信号通路、缺氧诱导因子-1(hypoxia-inducible factor-1,HIF-1)信号通路、IL-17信号通路及TNF信号通路等与AM的治疗密切相关。四逆失笑散加味方的关键活性成分quercetin(槲皮素)、kaempferol(山柰酚)、isorhamnetin(异鼠李素)、beta-sitosterol(β-谷甾醇)、sitosterol(谷甾醇)与核心靶点AKT1、IL-6、EGFR、TP53、TNF对接良好。结论:四逆失笑散加味方通过多成分、多靶点及多条信号通路协同作用治疗AM,其疗效可能与抗炎及抑制异位细胞增殖等过程密切相关。
