Quinoa is a good source of phenolics,which both exist as free and bound forms.In order to mark clear the characteristic free and bound phenolics in different quinoa samples,in this study,characteristic free and bound ...Quinoa is a good source of phenolics,which both exist as free and bound forms.In order to mark clear the characteristic free and bound phenolics in different quinoa samples,in this study,characteristic free and bound phenolics in three colored quinoas including WQ(white quinoa),RQ(red quinoa)and BQ(black quinoa)were investigated.Result showed a total of 14 phenolics both acted as free and bound form were analyzed in three colored quinoas(WQ,RQ and BQ).Gallic acid,vanillic acid,epicatechin,p-coumaric acid and quercetin existed both as free and bound forms were common phenolics in quinoas.The highest total free phenolics(238.10 mg/kg)and bound phenolics(3377.75 mg/kg)were presented in WQ and RQ,respectively.It indicated WQ and RQ were respectively good source of free and bound phenolics.Moreover,characteristic free and bound phenolics in three colored quinoas could be well analyzed by principal component analysis(PCA),indicating it was an effective and reliable method in distinguishing three colored quinoas based on their characteristic free and bound phenolics,respectively.展开更多
In this study the antioxidant activity of barley malt rootlet (BMR) extracts w</span><span style="font-family:"">as</span><span style="font-family:""> evaluated in...In this study the antioxidant activity of barley malt rootlet (BMR) extracts w</span><span style="font-family:"">as</span><span style="font-family:""> evaluated in heat treated corn oil up to 5 hours at 185</span><span style="font-family:"">°</span><span style="font-family:"">C frying temperature. The antioxidant activity </span><span style="font-family:"">of </span><span style="font-family:"">BMR extracts was measured at 25, 50, 100 and 150 ppm concentrations. The free and bound antioxidant phenolics were extracted from BMR using three different extraction methods. Conventional solvent extraction (CSE), microwave assisted extraction (MAE) and autoclave assisted pretreated solvent extraction (APSE). In the present experiment, the total phenolic content and antioxidant activity of the various extracts w</span><span style="font-family:"">ere</span><span style="font-family:""> measured. Thiobarbituric acid reactive substances (TBARS) assay was used to evaluate the ability of the BMR to protect lipid peroxidation in corn oil at 185</span><span style="font-family:"">°</span><span style="font-family:"">C frying temperature. The formation of TBARS at 5 hours of heat treated corn oil ha</span><span style="font-family:"">s</span><span style="font-family:""> shown similar antioxidant levels in 150 ppm butylated hydroxytoluene (BHT) or MAE free phenolic extract added to corn oil. TBARS value for BHT was 1.896 ± 0.013 μg/mL of corn oil and for MAE was 1.896 ± 0.034 μg/mL of corn oil. The highest level of antioxidant activity was found for the free phenolic extracts. The order of inhibition of oxidation was found to be for free phenolics as follows: BHT (100 ppm) > APSE (50 ppm) > MAE (100 ppm) > CSE (100 ppm).展开更多
Cinnamon is a traditional herbal medicine that is a valuable source of bioactive phenolic compounds.In this study,we determined the effects of cinnamon free phenolic extract(CFPE)on glucose transport in Caco-2 cells a...Cinnamon is a traditional herbal medicine that is a valuable source of bioactive phenolic compounds.In this study,we determined the effects of cinnamon free phenolic extract(CFPE)on glucose transport in Caco-2 cells and its possible mechanisms of action.First,the contents of phenolic compounds and flavonoids in bark were compared among four cinnamon cultivars.The‘Taiwan’cultivar had the highest contents of these compounds,and the CFPE was prepared from it.A Caco-2 monolayer was established,and the effect of CFPE on glucose transport across the monolayer was determined.The results showed that CFPE(at 150-300μg/mL)suppressed glucose transport across the monolayer in a dose-dependent manner.Procyanidin C1,procyanidin B2,procyanidin B1,procyanidin B3,procyanidin A2,and()-epicatechin derivatives in CFPE may have contributed to its suppressive effect.Treatment with CFPE decreased the transcript levels of SGK1 encoding serum and glucocorticoid-inducible kinase and PLC/PKC encoding phospholipase C/protein kinase C,which are involved in the signaling pathway associated with glucose transport;and the transcript levels of SGLT1 encoding sodium/glucose cotransporter 1 and GLUT2 encoding glucose transporter 2,the key glucose transporters in this pathway.These findings suggest that CFPE inhibits glucose transport via its effects on the signaling pathway and glucose transporters involved in glucose absorption in intestinal cells.Thus,CFPE has potential applications in preventing postprandial hyperglycemia.展开更多
Objectives:A comparative study regarding the fatty acid profiles and antioxidant properties of the two most common varieties of rice(Carnaroli and Karnak)cultivated in Sybaris(Southern Italy)was conducted.Carnaroli va...Objectives:A comparative study regarding the fatty acid profiles and antioxidant properties of the two most common varieties of rice(Carnaroli and Karnak)cultivated in Sybaris(Southern Italy)was conducted.Carnaroli variety from Pavia district(Northern Italy)was also investigated.Methods:Free and bound lipids were separately extracted.Fatty acid composition was determined by high resolution gas chromatography(HRGC).Free and bound phenolic acids were determined using high performance liquid chromatography(HPLC).The antioxidant properties were assessed using 2,2’-diphenyl-1-picrylhydrazyl(DPPH)radical scavenging assay.Results:The total lipid content was found to be higher in both Sybaris species with respect to the Pavia variety.The lipid distribution was also significantly different.In particular,the amount of bound lipids(14.2-16.3 mg g−1)was higher than the free lipids(7.2-7.5 mg g−1),and unsaturated fatty acids(USFA)were significantly higher in the free-FA fraction than in bound-FA in both Sybaris varieties.By contrast,Carnaroli variety from Northern Italy displayed a higher content of free(5.3 mg g−1)than bound lipids(2.5 mg g−1)and a higher content of USFA in the bound-FA.The antioxidant activity of Pavia rice extract was higher than that of both Sybaris varieties.Conclusions:The two Sybaris varieties are richer in lipids than the Pavia rice,while exhibiting the same qualitative fatty acid profile with some differences in the content of individual fatty acids.The distribution of lipids(free or bound)was found to be significantly different in the Carnaroli varieties,highlighting the effects of different climatic and pedological areas.展开更多
Thermomechanical process is the most commonly used for treating grains but inevitably cause loss of endogenous bioactive ingredients such as phenolics.This work investigated the phenolic retention of brown rice via bi...Thermomechanical process is the most commonly used for treating grains but inevitably cause loss of endogenous bioactive ingredients such as phenolics.This work investigated the phenolic retention of brown rice via bioextrusion at different concentration gradients of mesophilicα-amylase(MαA,0-2000 U/g),and determined the conversion of individual phenolic acids at both free and bound forms.Results showed that extrusion promoted the release of phenolics but lead to a great loss of them.While the phenolic retention of brown rice was significantly increased with increasing MαA levels up to 1000 U/g(twice more than that of traditional extrudate),which simultaneously modified rice matrix from big granules to small ones by SEM observation.The viscosity of bioextruded brown rice was decreased significantly but negatively relating to phenolic retention.Extrusion kinetics revealed that mechanical input and residence time had obvious effects on starch gelatinization which ultimately influence the presence of phenolics.展开更多
基金support of Youth Project of Liaoning Provincial Education Department(JYTQN2023334)Anhui Provincial Department of Education Key Research Project on Natural Science in Higher Education Institutions(KJ2021A1072)Science and Technology Plan Project of Chuzhou Science and Technology Bureau(2021ZD023).
文摘Quinoa is a good source of phenolics,which both exist as free and bound forms.In order to mark clear the characteristic free and bound phenolics in different quinoa samples,in this study,characteristic free and bound phenolics in three colored quinoas including WQ(white quinoa),RQ(red quinoa)and BQ(black quinoa)were investigated.Result showed a total of 14 phenolics both acted as free and bound form were analyzed in three colored quinoas(WQ,RQ and BQ).Gallic acid,vanillic acid,epicatechin,p-coumaric acid and quercetin existed both as free and bound forms were common phenolics in quinoas.The highest total free phenolics(238.10 mg/kg)and bound phenolics(3377.75 mg/kg)were presented in WQ and RQ,respectively.It indicated WQ and RQ were respectively good source of free and bound phenolics.Moreover,characteristic free and bound phenolics in three colored quinoas could be well analyzed by principal component analysis(PCA),indicating it was an effective and reliable method in distinguishing three colored quinoas based on their characteristic free and bound phenolics,respectively.
文摘In this study the antioxidant activity of barley malt rootlet (BMR) extracts w</span><span style="font-family:"">as</span><span style="font-family:""> evaluated in heat treated corn oil up to 5 hours at 185</span><span style="font-family:"">°</span><span style="font-family:"">C frying temperature. The antioxidant activity </span><span style="font-family:"">of </span><span style="font-family:"">BMR extracts was measured at 25, 50, 100 and 150 ppm concentrations. The free and bound antioxidant phenolics were extracted from BMR using three different extraction methods. Conventional solvent extraction (CSE), microwave assisted extraction (MAE) and autoclave assisted pretreated solvent extraction (APSE). In the present experiment, the total phenolic content and antioxidant activity of the various extracts w</span><span style="font-family:"">ere</span><span style="font-family:""> measured. Thiobarbituric acid reactive substances (TBARS) assay was used to evaluate the ability of the BMR to protect lipid peroxidation in corn oil at 185</span><span style="font-family:"">°</span><span style="font-family:"">C frying temperature. The formation of TBARS at 5 hours of heat treated corn oil ha</span><span style="font-family:"">s</span><span style="font-family:""> shown similar antioxidant levels in 150 ppm butylated hydroxytoluene (BHT) or MAE free phenolic extract added to corn oil. TBARS value for BHT was 1.896 ± 0.013 μg/mL of corn oil and for MAE was 1.896 ± 0.034 μg/mL of corn oil. The highest level of antioxidant activity was found for the free phenolic extracts. The order of inhibition of oxidation was found to be for free phenolics as follows: BHT (100 ppm) > APSE (50 ppm) > MAE (100 ppm) > CSE (100 ppm).
基金We acknowledge the Agricultural Competitive Industry Discipline Team Building Project of Guangdong Academy of Agricultural Sciences(202119TD)the Excellent Doctors Foundation of Guangdong Academy of Agricultural Sciences(R2021YJ-YB1002)+1 种基金the Guidance for Young Scientist of Guangdong Academy of Agricultural Sciences(R2020QD-043)the Innovative Research Team Construction Project for Modern Agricultural Industry Common Key Technologies of Guangdong Province(2022KJ117)for financial support.
文摘Cinnamon is a traditional herbal medicine that is a valuable source of bioactive phenolic compounds.In this study,we determined the effects of cinnamon free phenolic extract(CFPE)on glucose transport in Caco-2 cells and its possible mechanisms of action.First,the contents of phenolic compounds and flavonoids in bark were compared among four cinnamon cultivars.The‘Taiwan’cultivar had the highest contents of these compounds,and the CFPE was prepared from it.A Caco-2 monolayer was established,and the effect of CFPE on glucose transport across the monolayer was determined.The results showed that CFPE(at 150-300μg/mL)suppressed glucose transport across the monolayer in a dose-dependent manner.Procyanidin C1,procyanidin B2,procyanidin B1,procyanidin B3,procyanidin A2,and()-epicatechin derivatives in CFPE may have contributed to its suppressive effect.Treatment with CFPE decreased the transcript levels of SGK1 encoding serum and glucocorticoid-inducible kinase and PLC/PKC encoding phospholipase C/protein kinase C,which are involved in the signaling pathway associated with glucose transport;and the transcript levels of SGLT1 encoding sodium/glucose cotransporter 1 and GLUT2 encoding glucose transporter 2,the key glucose transporters in this pathway.These findings suggest that CFPE inhibits glucose transport via its effects on the signaling pathway and glucose transporters involved in glucose absorption in intestinal cells.Thus,CFPE has potential applications in preventing postprandial hyperglycemia.
文摘Objectives:A comparative study regarding the fatty acid profiles and antioxidant properties of the two most common varieties of rice(Carnaroli and Karnak)cultivated in Sybaris(Southern Italy)was conducted.Carnaroli variety from Pavia district(Northern Italy)was also investigated.Methods:Free and bound lipids were separately extracted.Fatty acid composition was determined by high resolution gas chromatography(HRGC).Free and bound phenolic acids were determined using high performance liquid chromatography(HPLC).The antioxidant properties were assessed using 2,2’-diphenyl-1-picrylhydrazyl(DPPH)radical scavenging assay.Results:The total lipid content was found to be higher in both Sybaris species with respect to the Pavia variety.The lipid distribution was also significantly different.In particular,the amount of bound lipids(14.2-16.3 mg g−1)was higher than the free lipids(7.2-7.5 mg g−1),and unsaturated fatty acids(USFA)were significantly higher in the free-FA fraction than in bound-FA in both Sybaris varieties.By contrast,Carnaroli variety from Northern Italy displayed a higher content of free(5.3 mg g−1)than bound lipids(2.5 mg g−1)and a higher content of USFA in the bound-FA.The antioxidant activity of Pavia rice extract was higher than that of both Sybaris varieties.Conclusions:The two Sybaris varieties are richer in lipids than the Pavia rice,while exhibiting the same qualitative fatty acid profile with some differences in the content of individual fatty acids.The distribution of lipids(free or bound)was found to be significantly different in the Carnaroli varieties,highlighting the effects of different climatic and pedological areas.
基金Natural Science Foundation of Zhejiang Province,China(LQ21C200008)Young Elite Scientists Sponsorship Program by CAST(2022QNRC001)+1 种基金the Natural Science Foundation of China(32001837 and 32272464)the National Key Research and Development Program of China(2022YFF1101800).
文摘Thermomechanical process is the most commonly used for treating grains but inevitably cause loss of endogenous bioactive ingredients such as phenolics.This work investigated the phenolic retention of brown rice via bioextrusion at different concentration gradients of mesophilicα-amylase(MαA,0-2000 U/g),and determined the conversion of individual phenolic acids at both free and bound forms.Results showed that extrusion promoted the release of phenolics but lead to a great loss of them.While the phenolic retention of brown rice was significantly increased with increasing MαA levels up to 1000 U/g(twice more than that of traditional extrudate),which simultaneously modified rice matrix from big granules to small ones by SEM observation.The viscosity of bioextruded brown rice was decreased significantly but negatively relating to phenolic retention.Extrusion kinetics revealed that mechanical input and residence time had obvious effects on starch gelatinization which ultimately influence the presence of phenolics.
