The carbonate-free fraction of 20 surface sediments collected from the ultraslow-spreading Southwest Indian Ridge(SWIR) was studied by grain size analysis and mineralogical analysis with X-ray powder diffraction(XRD),...The carbonate-free fraction of 20 surface sediments collected from the ultraslow-spreading Southwest Indian Ridge(SWIR) was studied by grain size analysis and mineralogical analysis with X-ray powder diffraction(XRD),stereo microscopy and scanning electron microscopy(SEM). The characteristics of the carbonate-free fraction of the sediments were obtained, and related influential factors were discussed. The results show that the mean grain size of this fraction is in 1.96Φ–8.19Φ, with poorly sorting and unimodal, bimodal or irregular bimodal distribution patterns. Four grain size end members of the fraction are derived with the End Member Model method. The finest end member EM1 shows a significant contribution of terrigenous materials of the aeolian input and sediment carried by the bottom current. End member EM2 with medium size mainly reflects sediment of a siliceous bioclast origin. EM3 and EM4 are interpreted as representing the coarser volcanic materials related to bedrock weathering or volcanic activities. Multi-provenance is the dominant factor controlling the grain size pattern of the carbonate-free fraction of the sediments in that area. In addition, sediment transport processes such as the bottom current and wind are the minor factors that influence the grain size distribution of the carbonate-free fraction sediments.展开更多
The molecular composition and biomarker distribution of various occurrences of organic matter in argillaceous source rocks developed in fresh and saline lacustrine environments were revealed by successive treatments o...The molecular composition and biomarker distribution of various occurrences of organic matter in argillaceous source rocks developed in fresh and saline lacustrine environments were revealed by successive treatments of solvent extraction followed by acid hydrolysis using gas chromatography–mass spectrometry. The free fraction obtained by solvent extraction provided abundant geochemical information concerning the sedimentary environment, thermal maturity and biogenic origin, and obvious differences existed between fresh and saline lacustrine source rock samples. Our research results indicate that the carbonate-mineral-bound(CM-bound) fraction released by successive acid hydrolysis could also serve as a significant biogenic indicator, as the bicyclic sesquiterpenoids, indicative of Botryococcus braunii origin, were specifically detected in quite high abundance in the acid-soluble fraction. In addition, the light end hydrocarbons were much better preserved in the acid-soluble fraction, and elemental sulfur was only detected in the CM-bound fraction, suggesting a relatively confined environment for the CM-bound fraction, which thus could preserve additional geochemical information compared to that of the free fraction. The CM-bound fraction also exhibited discernable differences between fresh and saline lacustrine samples. Therefore, it can be concluded that comprehensive analysis of free and CM-bound fractions in the argillaceous source rocks can provide a more authentic and objective interpretation of geologic conditions.展开更多
Effects of free iron oxyhydrates （Fed） and soil organic matter （SOM） on copper （Cu^2＋） sorption-desorption behavior by size fractions of aggregates from two typical paddy soils （Ferric-Accumulic Stagnic Anthro...Effects of free iron oxyhydrates （Fed） and soil organic matter （SOM） on copper （Cu^2＋） sorption-desorption behavior by size fractions of aggregates from two typical paddy soils （Ferric-Accumulic Stagnic Anthrosol （Soil H） and Gleyic Stagnic Anthrosol （Soil W）） were investigated with and without treatments of dithionite-citrate-bicarbonate and of H2O2. The size fractions of aggregates were obtained from the undisturbed bulk topsoil using a low energy ultrasonic dispersion procedure. Experiments of equilibrium sorption and subsequent desorption were conducted at soil water ratio of 1：20, 25℃. For Soil H, Cu^2＋ sorption capacity of the DCB-treated size fractions was decreased by 5.9% for fine sand fraction, by 40.4% for coarse sand fraction, in comparison to 2.9% for the bnlk sample. However, Cu^2＋ sorption capacities of the H2O2-treated fractions were decreased by over 80% for the coarse sand fraction and by 15% for the clay-sized fraction in comparison to 88% for bulk soil. For Soil W, Cu^2＋ sorption capacity of the DCB-treated size fraction was decreased by 30% for the coarse sand fraction and by over 75% for silt sand fraction in comparison to 44.5% for the bulk sample. Cu^2＋ sorption capacities of the H2O2-treated fractions were decreased by only 2.0% for the coarse sand fraction and by 15% for the fine sand fraction in comparison to by 3.4% for bulk soil. However, Cu^2＋ desorption rates were increased much in H2O2-treated samples by over 80% except the clay-sized fraction （only 9.5%） for Soil H. While removal of SOM with H2O2 tendend to increase the desorption rate, DCB- and H2O2-treatments caused decrease in Cu^2＋ retention capacity of size fractions, Particularly, there hardly remained Cu^2＋ retention capacity by size fractions from Soil H after H2O2 treatment except for clay-sized fraction. These findings supported again the dominance of the coarse sand fraction in sorption of metals and the preference of absorbed metals bound to SOM in differently stabilized status among the size fractions. Thus, enrichment and turnover of SOM in paddy soils may have great effects on metal retention and chemical mobility in paddy soils.展开更多
The present paper deals with the numerical solution of time-fractional partial differential equations using the element-free Galerkin （EFG） method, which is based on the moving least-square approximation. Compared w...The present paper deals with the numerical solution of time-fractional partial differential equations using the element-free Galerkin （EFG） method, which is based on the moving least-square approximation. Compared with numerical methods based on meshes, the EFG method for time-fractional partial differential equations needs only scattered nodes instead of meshing the domain of the problem. It neither requires element connectivity nor suffers much degradation in accuracy when nodal arrangements are very irregular. In this method, the first-order time derivative is replaced by the Caputo fractional derivative of order α（0 〈 α≤ 1）. The Galerkin weak form is used to obtain the discrete equations, and the essential boundary conditions are enforced by the penalty method. Several numerical examples are presented and the results we obtained are in good agreement with the exact solutions.展开更多
Anion-exchange membrane water electrolysers(AEMWEs)and fuel cells(AEMFCs)are critical technologies for converting renewable resources into green hydrogen(H_(2)),where anion-exchange membranes(AEMs)play a vital role in...Anion-exchange membrane water electrolysers(AEMWEs)and fuel cells(AEMFCs)are critical technologies for converting renewable resources into green hydrogen(H_(2)),where anion-exchange membranes(AEMs)play a vital role in efficiently transporting hydroxide ions(OH^(-))and minimizing fuel crossover,thus enhancing overall efficiency.While conventional AEMs with linear,side-chain,and block polymer architectures show promise through functionalization,their long-term performance remains a concern.To address this,hyperbranched polymers offer a promising alternative due to their three-dimensional structure,higher terminal functionality,and ease of functionalization.This unique architecture provides interconnected ion transport pathways,fractional free volume,and enhanced long-term stability in alkaline environments.Recent studies have achieved conductivities as high as 304.5 mS cm^(-1),attributed to their improved fractional free volume and microphase separation in hyperbranched AEMs.This review explores the chemical,mechanical,and ionic properties of hyperbranched AEMs in AEMFCs and assesses their potential for application in AEMWEs.Strategies such as blending and structural functionalisation have significantly improved the properties by promoting microphase separation and increasing the density of cationic groups on the polymer surface.The review provides essential insights for future research,highlighting the challenges and opportunities in developing high-performance hyperbranched AEMs to advance hydrogen energy infrastructure.展开更多
To capture the subdiffusive characteristics of financial markets, the subordinated process, directed by the inverse α-stale subordinator Sα （t） for 0 〈 α〈 1, has been employed as the model of asset prices. In t...To capture the subdiffusive characteristics of financial markets, the subordinated process, directed by the inverse α-stale subordinator Sα （t） for 0 〈 α〈 1, has been employed as the model of asset prices. In this article, we introduce a multidimensional subdiffusion model that has a bond and K correlated stocks. The stock price process is a multidimen- sional subdiffusion process directed by the inverse a-stable subordinator. This model describes the period of stagnation for each stock and the behavior of the dependency between multiple stocks. Moreover, we derive the multidimensional fractional backward Kolmogorov equation for the subordinated process using the Laplace transform technique. Finally, using a martingale approach, we prove that the multidimensional subdiffusion model is arbitrage-free, and also gives an arbitrage-free pricing rule for contingent claims associated with the martingale measure.展开更多
We obtain explicit expressions for one unknown thermal coefficient (among the conductivity, mass density, specific heat and latent heat of fusion) of a semi-infinite material through the one-phase fractional Lamé...We obtain explicit expressions for one unknown thermal coefficient (among the conductivity, mass density, specific heat and latent heat of fusion) of a semi-infinite material through the one-phase fractional Lamé-Clapeyron-Stefan problem with an over-specified boundary condition on the fixed face . The partial differential equation and one of the conditions on the free boundary include a time Caputo’s fractional derivative of order . Moreover, we obtain the necessary and sufficient conditions on data in order to have a unique solution by using recent results obtained for the fractional diffusion equation exploiting the properties of the Wright and Mainardi functions, given in: 1) Roscani-Santillan Marcus, Fract. Calc. Appl. Anal., 16 (2013), 802 - 815;2) Roscani-Tarzia, Adv. Math. Sci. Appl., 24 (2014), 237 - 249 and 3) Voller, Int. J. Heat Mass Transfer, 74 (2014), 269 - 277. This work generalizes the method developed for the determination of unknown thermal coefficients for the classical Lamé-Clapeyron-Stefan problem given in Tarzia, Adv. Appl. Math., 3 (1982), 74 - 82, which is recovered by taking the limit when the order .展开更多
Background and aim of work: We aimed from this study to determine the response and local relapse free survival (RFS) of pediatric patients with localized Ewing’s sarcoma treated with accelerated hyper-fractionated RT...Background and aim of work: We aimed from this study to determine the response and local relapse free survival (RFS) of pediatric patients with localized Ewing’s sarcoma treated with accelerated hyper-fractionated RT. Patient and methods: This study was a nonrandomized uncontrolled phase II study and was conducted at clinical oncology department and south Egypt cancer institute;it involved 28 patients with histologically confirmed Ewing’s sarcoma;all of them were subjected to PET/CT whenever possible or MRI with contrast of the primary site, MSCT chest, bone scan, and LDH to ensure absence of metastasis followed by the protocol of accelerated hyper fractionated RT. Results: The overall response rate (ORR) was 92.9% by MRI with significant effect of the type of response on local RFS (P 0.002). The median local RFS of 28 patients with localized Ewing’s sarcoma was 30 ± 8.599 months with 95% CI = 13.147 - 46.853;the 3-year local control was 35%. Conclusion: Accelerated hyper fractionated RT didn’t achieve better results than standard fractionation RT, but it is recommended to be done on a large sample size, and multiple centers, and continued follow up is also recommended to evaluate 5-year LRFS, 5-year OS.展开更多
Coronary angiography is considered to be the gold standard in the morphological evaluation of coronary artery stenosis. The morphological assessment of the severity of a coronary lesion is very subjective. Thus, the i...Coronary angiography is considered to be the gold standard in the morphological evaluation of coronary artery stenosis. The morphological assessment of the severity of a coronary lesion is very subjective. Thus, the invasive fractional flow reserve(FFR) measurement represents the current standard for estimation of the hemodynamic significance of coronary artery stenosis. The FFR-guided revascularization strategy was initially classified as a Class-IA-recommendation in the 2014 European Society of Cardiology/European Association for Cardio-Thoracic Surgery guidelines on myocardial revascularization. Both the Deferral vs Performance of Percutaneous Coronary Intervention of Functionally Non-Significant Coronary Stenosis and Flow Reserve vs Angiography for Multivessel Evaluation studies showed no treatment advantage for hemodynamically insignificant stenoses. With the help of FFR(and targeted interventions), clinical results could be improved; however, the use in clinical practice is still limited due to the need of adenosine administration and a significant prolongation of the length of the procedure. Instantaneous wave-free ratio(iFR~) is a new innovative approach for the determination of the hemodynamic significance of coronary stenosis, which can be obtained at rest without the use of vasodilators. Regarding the periprocedural complications as well as prognosis, iFR~ showed non-inferiority to FFR in the SWEDEHEART and DEFINE-FLAIR trials. Furthermore, iFR~, enhanced by iFR~-pullback, provides the possibility to display the iFR~-change over the course of the vessel to create a hemodynamic map.展开更多
目的应用非标记定量技术研究糖尿病患者血清中差异表达蛋白,筛选特异性蛋白质标志物。方法收集2023年深圳市糖尿病患者和健康对照者的血清各3例,应用免疫亲和层析和纳升液相色谱-四极杆-静电场轨道线性离子阱三合一质谱法,利用分级(frac...目的应用非标记定量技术研究糖尿病患者血清中差异表达蛋白,筛选特异性蛋白质标志物。方法收集2023年深圳市糖尿病患者和健康对照者的血清各3例,应用免疫亲和层析和纳升液相色谱-四极杆-静电场轨道线性离子阱三合一质谱法,利用分级(fraction,FC)和不分级(no fraction,NF)2种方法对糖尿病患者和健康对照者的血清蛋白质组进行非标记定量分析,质谱数据经MaxQuant1.6.1.0进行检索,对分级方法鉴定的差异蛋白质进行GO(gene oncology)功能聚类分析和KEGG(kyoto encyclopedia of genes and genomes)通路分析。结果未分级方法鉴定可信蛋白251种,筛选差异蛋白17种;分级方法鉴定可信蛋白494种,筛选差异蛋白74种。其中7种蛋白在2种方法中变化趋势完全一致。有5种蛋白下调,分别是妊娠区带蛋白(pregnancy zone protein,PZP)、碳酸酐酶1(carbonic anhydrase 1,CAH1)、甘露糖结合蛋白C(mannose-binding protein C,MBL2)、纤维蛋白原α链(fibrinogen alpha chain,FIBA)和触珠蛋白(haptoglobin,HPT);2种蛋白上调,分别是脂蛋白a[apolipoprotein(a),LPA]和补体因子H相关蛋白4(complement fac‐tor H-related protein 4,CFH4)。GO分析显示差异蛋白的分子功能主要集中在糖胺聚糖结合、肝素结合等过程。KEGG通路分析显示差异蛋白主要集中在补体和级联凝血、细胞黏附、PI3K-Akt信号通路等通路上。结论基于分级的非标记定量蛋白质组学分析技术可有效分析糖尿病患者血清蛋白的差异表达,为筛选大规模队列疾病血清样本的特异性蛋白质标志物提供了有效技术手段。展开更多
基金China Ocean Mineral Resources Research and Development under contract No.DY125-11-R-04the National Natural Science Foundation of China under contract No.41676036
文摘The carbonate-free fraction of 20 surface sediments collected from the ultraslow-spreading Southwest Indian Ridge(SWIR) was studied by grain size analysis and mineralogical analysis with X-ray powder diffraction(XRD),stereo microscopy and scanning electron microscopy(SEM). The characteristics of the carbonate-free fraction of the sediments were obtained, and related influential factors were discussed. The results show that the mean grain size of this fraction is in 1.96Φ–8.19Φ, with poorly sorting and unimodal, bimodal or irregular bimodal distribution patterns. Four grain size end members of the fraction are derived with the End Member Model method. The finest end member EM1 shows a significant contribution of terrigenous materials of the aeolian input and sediment carried by the bottom current. End member EM2 with medium size mainly reflects sediment of a siliceous bioclast origin. EM3 and EM4 are interpreted as representing the coarser volcanic materials related to bedrock weathering or volcanic activities. Multi-provenance is the dominant factor controlling the grain size pattern of the carbonate-free fraction of the sediments in that area. In addition, sediment transport processes such as the bottom current and wind are the minor factors that influence the grain size distribution of the carbonate-free fraction sediments.
基金funded by NSFC (National Science Foundation of China) No. 41372130
文摘The molecular composition and biomarker distribution of various occurrences of organic matter in argillaceous source rocks developed in fresh and saline lacustrine environments were revealed by successive treatments of solvent extraction followed by acid hydrolysis using gas chromatography–mass spectrometry. The free fraction obtained by solvent extraction provided abundant geochemical information concerning the sedimentary environment, thermal maturity and biogenic origin, and obvious differences existed between fresh and saline lacustrine source rock samples. Our research results indicate that the carbonate-mineral-bound(CM-bound) fraction released by successive acid hydrolysis could also serve as a significant biogenic indicator, as the bicyclic sesquiterpenoids, indicative of Botryococcus braunii origin, were specifically detected in quite high abundance in the acid-soluble fraction. In addition, the light end hydrocarbons were much better preserved in the acid-soluble fraction, and elemental sulfur was only detected in the CM-bound fraction, suggesting a relatively confined environment for the CM-bound fraction, which thus could preserve additional geochemical information compared to that of the free fraction. The CM-bound fraction also exhibited discernable differences between fresh and saline lacustrine samples. Therefore, it can be concluded that comprehensive analysis of free and CM-bound fractions in the argillaceous source rocks can provide a more authentic and objective interpretation of geologic conditions.
基金supported by the National Natural Science Foundation of China (No. 40231016).
文摘Effects of free iron oxyhydrates （Fed） and soil organic matter （SOM） on copper （Cu^2＋） sorption-desorption behavior by size fractions of aggregates from two typical paddy soils （Ferric-Accumulic Stagnic Anthrosol （Soil H） and Gleyic Stagnic Anthrosol （Soil W）） were investigated with and without treatments of dithionite-citrate-bicarbonate and of H2O2. The size fractions of aggregates were obtained from the undisturbed bulk topsoil using a low energy ultrasonic dispersion procedure. Experiments of equilibrium sorption and subsequent desorption were conducted at soil water ratio of 1：20, 25℃. For Soil H, Cu^2＋ sorption capacity of the DCB-treated size fractions was decreased by 5.9% for fine sand fraction, by 40.4% for coarse sand fraction, in comparison to 2.9% for the bnlk sample. However, Cu^2＋ sorption capacities of the H2O2-treated fractions were decreased by over 80% for the coarse sand fraction and by 15% for the clay-sized fraction in comparison to 88% for bulk soil. For Soil W, Cu^2＋ sorption capacity of the DCB-treated size fraction was decreased by 30% for the coarse sand fraction and by over 75% for silt sand fraction in comparison to 44.5% for the bulk sample. Cu^2＋ sorption capacities of the H2O2-treated fractions were decreased by only 2.0% for the coarse sand fraction and by 15% for the fine sand fraction in comparison to by 3.4% for bulk soil. However, Cu^2＋ desorption rates were increased much in H2O2-treated samples by over 80% except the clay-sized fraction （only 9.5%） for Soil H. While removal of SOM with H2O2 tendend to increase the desorption rate, DCB- and H2O2-treatments caused decrease in Cu^2＋ retention capacity of size fractions, Particularly, there hardly remained Cu^2＋ retention capacity by size fractions from Soil H after H2O2 treatment except for clay-sized fraction. These findings supported again the dominance of the coarse sand fraction in sorption of metals and the preference of absorbed metals bound to SOM in differently stabilized status among the size fractions. Thus, enrichment and turnover of SOM in paddy soils may have great effects on metal retention and chemical mobility in paddy soils.
基金Project supported by the National Natural Science Foundation of China(Grant No.11072117)the Natural Science Foundationof Zhejiang Province,China(Grant Nos.Y6110007and Y6110502)the K.C.Wong Magna Fund in Ningbo University,China
文摘The present paper deals with the numerical solution of time-fractional partial differential equations using the element-free Galerkin （EFG） method, which is based on the moving least-square approximation. Compared with numerical methods based on meshes, the EFG method for time-fractional partial differential equations needs only scattered nodes instead of meshing the domain of the problem. It neither requires element connectivity nor suffers much degradation in accuracy when nodal arrangements are very irregular. In this method, the first-order time derivative is replaced by the Caputo fractional derivative of order α（0 〈 α≤ 1）. The Galerkin weak form is used to obtain the discrete equations, and the essential boundary conditions are enforced by the penalty method. Several numerical examples are presented and the results we obtained are in good agreement with the exact solutions.
基金UKRI financial support under grant number EP/Y026098/1 for Global Hydrogen Production Technologies(HyPT)Center。
文摘Anion-exchange membrane water electrolysers(AEMWEs)and fuel cells(AEMFCs)are critical technologies for converting renewable resources into green hydrogen(H_(2)),where anion-exchange membranes(AEMs)play a vital role in efficiently transporting hydroxide ions(OH^(-))and minimizing fuel crossover,thus enhancing overall efficiency.While conventional AEMs with linear,side-chain,and block polymer architectures show promise through functionalization,their long-term performance remains a concern.To address this,hyperbranched polymers offer a promising alternative due to their three-dimensional structure,higher terminal functionality,and ease of functionalization.This unique architecture provides interconnected ion transport pathways,fractional free volume,and enhanced long-term stability in alkaline environments.Recent studies have achieved conductivities as high as 304.5 mS cm^(-1),attributed to their improved fractional free volume and microphase separation in hyperbranched AEMs.This review explores the chemical,mechanical,and ionic properties of hyperbranched AEMs in AEMFCs and assesses their potential for application in AEMWEs.Strategies such as blending and structural functionalisation have significantly improved the properties by promoting microphase separation and increasing the density of cationic groups on the polymer surface.The review provides essential insights for future research,highlighting the challenges and opportunities in developing high-performance hyperbranched AEMs to advance hydrogen energy infrastructure.
基金Supported by National Outstanding Youth Science Foundation （61125306）, Major Research Plan of National Natural Science Foundation of China （91016004）, National Natural Science Foundation （61203071）, Fundamental Research Funds for the Central Universities （FRF-TP-13-017A）, and Specialized Research Fund for the Doctoral Program of Higher Education （20130006120027, 20110092110020）
基金Project supported by the National Natural Science Foundation of China(Grant No.11171238)
文摘To capture the subdiffusive characteristics of financial markets, the subordinated process, directed by the inverse α-stale subordinator Sα （t） for 0 〈 α〈 1, has been employed as the model of asset prices. In this article, we introduce a multidimensional subdiffusion model that has a bond and K correlated stocks. The stock price process is a multidimen- sional subdiffusion process directed by the inverse a-stable subordinator. This model describes the period of stagnation for each stock and the behavior of the dependency between multiple stocks. Moreover, we derive the multidimensional fractional backward Kolmogorov equation for the subordinated process using the Laplace transform technique. Finally, using a martingale approach, we prove that the multidimensional subdiffusion model is arbitrage-free, and also gives an arbitrage-free pricing rule for contingent claims associated with the martingale measure.
文摘We obtain explicit expressions for one unknown thermal coefficient (among the conductivity, mass density, specific heat and latent heat of fusion) of a semi-infinite material through the one-phase fractional Lamé-Clapeyron-Stefan problem with an over-specified boundary condition on the fixed face . The partial differential equation and one of the conditions on the free boundary include a time Caputo’s fractional derivative of order . Moreover, we obtain the necessary and sufficient conditions on data in order to have a unique solution by using recent results obtained for the fractional diffusion equation exploiting the properties of the Wright and Mainardi functions, given in: 1) Roscani-Santillan Marcus, Fract. Calc. Appl. Anal., 16 (2013), 802 - 815;2) Roscani-Tarzia, Adv. Math. Sci. Appl., 24 (2014), 237 - 249 and 3) Voller, Int. J. Heat Mass Transfer, 74 (2014), 269 - 277. This work generalizes the method developed for the determination of unknown thermal coefficients for the classical Lamé-Clapeyron-Stefan problem given in Tarzia, Adv. Appl. Math., 3 (1982), 74 - 82, which is recovered by taking the limit when the order .
文摘Background and aim of work: We aimed from this study to determine the response and local relapse free survival (RFS) of pediatric patients with localized Ewing’s sarcoma treated with accelerated hyper-fractionated RT. Patient and methods: This study was a nonrandomized uncontrolled phase II study and was conducted at clinical oncology department and south Egypt cancer institute;it involved 28 patients with histologically confirmed Ewing’s sarcoma;all of them were subjected to PET/CT whenever possible or MRI with contrast of the primary site, MSCT chest, bone scan, and LDH to ensure absence of metastasis followed by the protocol of accelerated hyper fractionated RT. Results: The overall response rate (ORR) was 92.9% by MRI with significant effect of the type of response on local RFS (P 0.002). The median local RFS of 28 patients with localized Ewing’s sarcoma was 30 ± 8.599 months with 95% CI = 13.147 - 46.853;the 3-year local control was 35%. Conclusion: Accelerated hyper fractionated RT didn’t achieve better results than standard fractionation RT, but it is recommended to be done on a large sample size, and multiple centers, and continued follow up is also recommended to evaluate 5-year LRFS, 5-year OS.
文摘Coronary angiography is considered to be the gold standard in the morphological evaluation of coronary artery stenosis. The morphological assessment of the severity of a coronary lesion is very subjective. Thus, the invasive fractional flow reserve(FFR) measurement represents the current standard for estimation of the hemodynamic significance of coronary artery stenosis. The FFR-guided revascularization strategy was initially classified as a Class-IA-recommendation in the 2014 European Society of Cardiology/European Association for Cardio-Thoracic Surgery guidelines on myocardial revascularization. Both the Deferral vs Performance of Percutaneous Coronary Intervention of Functionally Non-Significant Coronary Stenosis and Flow Reserve vs Angiography for Multivessel Evaluation studies showed no treatment advantage for hemodynamically insignificant stenoses. With the help of FFR(and targeted interventions), clinical results could be improved; however, the use in clinical practice is still limited due to the need of adenosine administration and a significant prolongation of the length of the procedure. Instantaneous wave-free ratio(iFR~) is a new innovative approach for the determination of the hemodynamic significance of coronary stenosis, which can be obtained at rest without the use of vasodilators. Regarding the periprocedural complications as well as prognosis, iFR~ showed non-inferiority to FFR in the SWEDEHEART and DEFINE-FLAIR trials. Furthermore, iFR~, enhanced by iFR~-pullback, provides the possibility to display the iFR~-change over the course of the vessel to create a hemodynamic map.
文摘目的应用非标记定量技术研究糖尿病患者血清中差异表达蛋白,筛选特异性蛋白质标志物。方法收集2023年深圳市糖尿病患者和健康对照者的血清各3例,应用免疫亲和层析和纳升液相色谱-四极杆-静电场轨道线性离子阱三合一质谱法,利用分级(fraction,FC)和不分级(no fraction,NF)2种方法对糖尿病患者和健康对照者的血清蛋白质组进行非标记定量分析,质谱数据经MaxQuant1.6.1.0进行检索,对分级方法鉴定的差异蛋白质进行GO(gene oncology)功能聚类分析和KEGG(kyoto encyclopedia of genes and genomes)通路分析。结果未分级方法鉴定可信蛋白251种,筛选差异蛋白17种;分级方法鉴定可信蛋白494种,筛选差异蛋白74种。其中7种蛋白在2种方法中变化趋势完全一致。有5种蛋白下调,分别是妊娠区带蛋白(pregnancy zone protein,PZP)、碳酸酐酶1(carbonic anhydrase 1,CAH1)、甘露糖结合蛋白C(mannose-binding protein C,MBL2)、纤维蛋白原α链(fibrinogen alpha chain,FIBA)和触珠蛋白(haptoglobin,HPT);2种蛋白上调,分别是脂蛋白a[apolipoprotein(a),LPA]和补体因子H相关蛋白4(complement fac‐tor H-related protein 4,CFH4)。GO分析显示差异蛋白的分子功能主要集中在糖胺聚糖结合、肝素结合等过程。KEGG通路分析显示差异蛋白主要集中在补体和级联凝血、细胞黏附、PI3K-Akt信号通路等通路上。结论基于分级的非标记定量蛋白质组学分析技术可有效分析糖尿病患者血清蛋白的差异表达,为筛选大规模队列疾病血清样本的特异性蛋白质标志物提供了有效技术手段。
