[Objectives]To analyze the chemical components of Meconopsis integrifolia(Maxim.)Franch total flavonoids(MITF).[Methods]The chemical components of MITF were identified by UPLC-Q-Exactive Orbitrap MS.The column was per...[Objectives]To analyze the chemical components of Meconopsis integrifolia(Maxim.)Franch total flavonoids(MITF).[Methods]The chemical components of MITF were identified by UPLC-Q-Exactive Orbitrap MS.The column was performed using ACQUITY UPLC HSS T3(100 mm×2.1 mm,1.8μm)with 0.1%formic acid water(A)-acetonitrile(B)with gradient elution at 30℃,injection volume of 2μL,and flow rate of 0.3 mL/min.Electrospray ion source adopted positive and negative ion detection mode with scanning range m/z 100-1000.[Results]A total of 93 compounds were identified from MITF,including 57 flavonoids,25 phenolic acids,9 alkaloids and 2 others.Among them,dimethoxytaxifolin was identified as a possible new compound by SciFinder search,and 67 compounds were first identified in M.integrifolia.[Conclusions]This study provides a scientific foundation for clarifying the material basis of the efficacy of M.integrifolia and improving the quality standards.展开更多
[Objective] This study aimed at comparing the four extraction methods of genomic DNA from Clematis fasciculiflora Franch and determining the optimal extraction method for extracting the genomic DNA from Clematis fasci...[Objective] This study aimed at comparing the four extraction methods of genomic DNA from Clematis fasciculiflora Franch and determining the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch.[Method] Leavies of Clematis fasciculiflora Franch were used as materials for comparing the purity and concentration of extracted DNA and extracting time among the four extraction methods of genomic DNA including improved CTAB method Ⅰ,improved CTAB method Ⅱ,improved CTAB method Ⅲ and improved SDS method.[Result] The four extraction methods could all be successfully used for extracting the genomic DNA from Clematis fasciculiflora Franch.The purity of genomic DNA was the highest using improved CTAB method Ⅰ,with the longest extracting time;while the concentration of genomic DNA was the maximum using the improved SDS method,with the shortest extracting time and relatively low purity;the extracting time of improved CTAB method Ⅲ was the shortest.[Conclusion] This study had established the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch and supported for the further research using molecular biological methods.展开更多
This paper aims to study characteristics and biodiversity of the Torreya fargesii community in the Jinfo Mountains to provide baseline information related to the protection of T. fargesii. The results indicated that a...This paper aims to study characteristics and biodiversity of the Torreya fargesii community in the Jinfo Mountains to provide baseline information related to the protection of T. fargesii. The results indicated that among the 5 plots(100 m2 each) set along an altitudinal gradient, 176 species of vascular plants, 136 genera and 64 families were recorded. These included 7 species of pteridophytes, 6 genera and 4 families, 3 species of gymnosperms, 3 genera and 2 families, and 166 species of angiosperms, 127 genera and 58 families. The community physiognomy was mainly dominated by phanerophytes with medium-sized herbaceous and simple leaves. The proportions of phanerophytes among the fl oristic spectrum, frequency spectrum and vegetational spectrum are 68.75%, 73.13% and 67.48% respectively. The vertical structure can be divided into the shrub and herb layers in this community. The dominant species in shrub layer were T. fargesii, Zanthoxylum ovalifolium, Rubus coreanus, Kerria japonica, Cotoneaster horizontalis etc.. The dominant herbs included Miscanthus sinensis, Carex gibba, Artemisia lactifl ora, Pteridium aquilinum var. latiusculum, Pilea notata, Aster ageratoides etc.. Species richness(S) and the Simpson(D), Shannon-Wiener(H') and Pielou evenness(Jsw) indices of shrub layer species richness are higher than those of the herb layer; the distribution of the species in the community is obviously uneven. The species richness and diversity indices increase with the rising altitude.展开更多
Two new oleanane-type triterpenoid saponins.sateryunn3noside A and B,were isolated from the roots of Aster yunnanensis Franch,and their Structures were elucidated based on spectral evidence and chemical hydrolysis. Th...Two new oleanane-type triterpenoid saponins.sateryunn3noside A and B,were isolated from the roots of Aster yunnanensis Franch,and their Structures were elucidated based on spectral evidence and chemical hydrolysis. They have the same aglycone,Which has been assigned as 2a,3 .23-trihydroxyolean-12-en-28-cio acid,and the deference consisted only in 28-O-linked sugar moieties. Asteryunnanoside A is a 28-O-a-L-rhamnopyranosyl-(1-2)- -D-glucopyranoside and B is 28-O- -D-glucopyranosyl-(1 -2),-D-glucopyranoside of the corresponding aglycone.展开更多
[Objectives] To determine the content of 10 kinds of inorganic elements(Pb,Cu,As,Cd,Hg,Zn,Fe,Mn,Sr and Cr) in samples of Paris polyphylla Smith var. yunnanensis( Franch.) Hand.-Mazz.( and polygerm varieties) produced ...[Objectives] To determine the content of 10 kinds of inorganic elements(Pb,Cu,As,Cd,Hg,Zn,Fe,Mn,Sr and Cr) in samples of Paris polyphylla Smith var. yunnanensis( Franch.) Hand.-Mazz.( and polygerm varieties) produced in different areas. [Methods] The wet digestion and technique of inductively coupled plasma optical emission spectrometry( ICP-OES) were used. [Results] Under the experimental conditions,elements were not related to each other,and several kinds of elements could be measured at the same time. The content of Pb exceeding the standard only in sample S4 of P. polyphylla Smith var. yunnanensis( and polygerm varieties),Hg was not detected in all samples,and the rest heavy metals did not exceed the limit. [Conclusions] This study was expected to provide a certain reference for the development and utilization of P. polyphylla Smith var. yunnanensis( and polygerm varieties).展开更多
[Objectives] To make original plant and microscopic identification and study the traits of Paris polyphylla Smith var. yunnanensis( Franch.) Hand.-Mazz.( polygerm varieties) produced in Gaoligong Mountain areas,and pr...[Objectives] To make original plant and microscopic identification and study the traits of Paris polyphylla Smith var. yunnanensis( Franch.) Hand.-Mazz.( polygerm varieties) produced in Gaoligong Mountain areas,and provide experimental data for their identification and application. [Methods] The original plant powder was used,and paraffin sections and physicochemical properties were identified. [Results]P. polyphylla Smith var. yunnanensis( polygerm varieties) produced in Gaoligong Mountain areas showed obvious microscopic features and physicochemical properties. [Conclusions] This study was expected to provide certain reference for identification of original plant and medicine and formulation of quality standard for P. polyphylla Smith var. yunnanensis( polygerm varieties) produced in Gaoligong Mountain areas,and provided basis for their further research and development.展开更多
Coptis chinensis Franch, a widely used Traditional Chinese Medicine, shows various kinds of bioactivity. The major active components of the herb are considered to be alkaloids. Thus, preparative separation of these al...Coptis chinensis Franch, a widely used Traditional Chinese Medicine, shows various kinds of bioactivity. The major active components of the herb are considered to be alkaloids. Thus, preparative separation of these alkaloids is critical important for further pharmacology and mechanism studies. In the paper, five alkaloids from C. chinensis were purified by HSCCC using the solvent system composed of chloro-form-metha- nol-water (2:1:1, v/v/v) single prepared. The content of each solvent in solvent system were determined by gas chromatography (GC), then according the ratios of solvents in each phase to prepare the mobile and stationary phase respectively. And a comparative study was carried out between together preparation and single preparation of the solvent system. The purities and recoveries of all the products were over 98.5% and 92%. However, 134 mL chloroform, 336 mL methanol and 452 mL water were saved when the two phase were singled by GC. Our research showed an economical method for separating alkaloids from C. chinensis by HSCCC using the solvent system single prepared by GC.展开更多
A new oleanane-type triterpene saponin. asteryunnanoside E. has been isolated from the roots of Astir yunnanensis Franch. and its structure elucidated as 3-O-β-D-glucopyranosyl-bayogenin-28-O-β-glucopyranoside by me...A new oleanane-type triterpene saponin. asteryunnanoside E. has been isolated from the roots of Astir yunnanensis Franch. and its structure elucidated as 3-O-β-D-glucopyranosyl-bayogenin-28-O-β-glucopyranoside by means of spectral data particularly 2D NMR including COSY. HETCOR, HOHAHA and ROESY techniques and chemical transformation.展开更多
Objective:To investigate the effect of decocted Coptis chinensis Franch.(Huanglian Jianji,黄连煎剂,HLJJ) and berbenne on the gut microbiota of the rats with metabolic syndrome (MS) induced by high-fat diet (HFD).Metho...Objective:To investigate the effect of decocted Coptis chinensis Franch.(Huanglian Jianji,黄连煎剂,HLJJ) and berbenne on the gut microbiota of the rats with metabolic syndrome (MS) induced by high-fat diet (HFD).Methods:Sixty Sprague Dawley rats were randomly divided into normal diet (ND) group (n =15)and HFD group (n =45).After 8 weeks of feeding,the HFD group was further divided into three groups:a group provided with a combination of HLJJ and HFD for 4 weeks,a group provided with a combination of berberine and HFD for 4 weeks,and a group supplied only with HFD for 4 consecutive weeks.Epididymal fat weight,serum high-density lipoproteincholesterohC (HDL-C),lowdensity lipoproteincholesterol-C (LDL-C) and irisin levels were measured.In addition,the V3-V4 region of the cecum microbiota 16S rRNA gene amplicon was sequenced on the Illumina MiSeq platform.Results:MS was successfully induced in the rats with HFD for 8 weeks.After 12 weeks,serum irisin levels in the HFD + berberine group were significantly increased compared with those in the HFD group (P <.05).The high-quality tags were delineated into 1149 operational taxonomic units at a 97% similarity level.Furthermore,at the genus level,Akkermansia muciniphila (A.muciniphila),Bacteroides,and Ruminococcus were markedly increased,whereas Candidatus arthromitus,Prevotella,Phascolarctobacterium were in great decrease in the HLJJ group and the berberine group,compared with the HFD group.The abundance of A.muciniphila,Ruminococcus gnavus and Bacteroides was significantly higher in the berberine group,whereas,the abundance of Oscillospira was lower in the berberine group,compared with the HLJJ group.Conclusion:HLJJ and berberine changed the gut microbiota in rats with MS.The mechanism underlying the treatments of berberine and HLJJ on HFD-induced MS is partly different.展开更多
基金Supported by Natural Science Foundation of Sichuan Province(2023NS-FSC0610)the Special Fund of Natural Science for the Central Universities of Southwest Minzu University(ZYN2023071).
文摘[Objectives]To analyze the chemical components of Meconopsis integrifolia(Maxim.)Franch total flavonoids(MITF).[Methods]The chemical components of MITF were identified by UPLC-Q-Exactive Orbitrap MS.The column was performed using ACQUITY UPLC HSS T3(100 mm×2.1 mm,1.8μm)with 0.1%formic acid water(A)-acetonitrile(B)with gradient elution at 30℃,injection volume of 2μL,and flow rate of 0.3 mL/min.Electrospray ion source adopted positive and negative ion detection mode with scanning range m/z 100-1000.[Results]A total of 93 compounds were identified from MITF,including 57 flavonoids,25 phenolic acids,9 alkaloids and 2 others.Among them,dimethoxytaxifolin was identified as a possible new compound by SciFinder search,and 67 compounds were first identified in M.integrifolia.[Conclusions]This study provides a scientific foundation for clarifying the material basis of the efficacy of M.integrifolia and improving the quality standards.
基金Supported by Applied Basic Research Project of Yunnan Province(2010ZC089)the948Project of National Forestry Bureau(2008-4-11)+1 种基金Sharing Platform Project of Provincial and Ministerial Key Subject,Key Laboratory and School Laboratory of Provincial Colleges and Universities in Yunnan ProvinceScience and Technology Innovation Fund of Southwest Forestry University~~
文摘[Objective] This study aimed at comparing the four extraction methods of genomic DNA from Clematis fasciculiflora Franch and determining the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch.[Method] Leavies of Clematis fasciculiflora Franch were used as materials for comparing the purity and concentration of extracted DNA and extracting time among the four extraction methods of genomic DNA including improved CTAB method Ⅰ,improved CTAB method Ⅱ,improved CTAB method Ⅲ and improved SDS method.[Result] The four extraction methods could all be successfully used for extracting the genomic DNA from Clematis fasciculiflora Franch.The purity of genomic DNA was the highest using improved CTAB method Ⅰ,with the longest extracting time;while the concentration of genomic DNA was the maximum using the improved SDS method,with the shortest extracting time and relatively low purity;the extracting time of improved CTAB method Ⅲ was the shortest.[Conclusion] This study had established the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch and supported for the further research using molecular biological methods.
基金Supported by National Natural Science Foundation of China(31470568)Project of Scientifi c Research Innovation Team of Yangtze Normal University(2014XJTD06)
文摘This paper aims to study characteristics and biodiversity of the Torreya fargesii community in the Jinfo Mountains to provide baseline information related to the protection of T. fargesii. The results indicated that among the 5 plots(100 m2 each) set along an altitudinal gradient, 176 species of vascular plants, 136 genera and 64 families were recorded. These included 7 species of pteridophytes, 6 genera and 4 families, 3 species of gymnosperms, 3 genera and 2 families, and 166 species of angiosperms, 127 genera and 58 families. The community physiognomy was mainly dominated by phanerophytes with medium-sized herbaceous and simple leaves. The proportions of phanerophytes among the fl oristic spectrum, frequency spectrum and vegetational spectrum are 68.75%, 73.13% and 67.48% respectively. The vertical structure can be divided into the shrub and herb layers in this community. The dominant species in shrub layer were T. fargesii, Zanthoxylum ovalifolium, Rubus coreanus, Kerria japonica, Cotoneaster horizontalis etc.. The dominant herbs included Miscanthus sinensis, Carex gibba, Artemisia lactifl ora, Pteridium aquilinum var. latiusculum, Pilea notata, Aster ageratoides etc.. Species richness(S) and the Simpson(D), Shannon-Wiener(H') and Pielou evenness(Jsw) indices of shrub layer species richness are higher than those of the herb layer; the distribution of the species in the community is obviously uneven. The species richness and diversity indices increase with the rising altitude.
文摘Two new oleanane-type triterpenoid saponins.sateryunn3noside A and B,were isolated from the roots of Aster yunnanensis Franch,and their Structures were elucidated based on spectral evidence and chemical hydrolysis. They have the same aglycone,Which has been assigned as 2a,3 .23-trihydroxyolean-12-en-28-cio acid,and the deference consisted only in 28-O-linked sugar moieties. Asteryunnanoside A is a 28-O-a-L-rhamnopyranosyl-(1-2)- -D-glucopyranoside and B is 28-O- -D-glucopyranosyl-(1 -2),-D-glucopyranoside of the corresponding aglycone.
基金Supported by Key Sci-Tech Project of Sichuan Provincial Science and Technology Department(2016FZ0045)Fundamental Research Funds for the Central Universities of Southwest Minzu University(2018NZD10)
文摘[Objectives] To determine the content of 10 kinds of inorganic elements(Pb,Cu,As,Cd,Hg,Zn,Fe,Mn,Sr and Cr) in samples of Paris polyphylla Smith var. yunnanensis( Franch.) Hand.-Mazz.( and polygerm varieties) produced in different areas. [Methods] The wet digestion and technique of inductively coupled plasma optical emission spectrometry( ICP-OES) were used. [Results] Under the experimental conditions,elements were not related to each other,and several kinds of elements could be measured at the same time. The content of Pb exceeding the standard only in sample S4 of P. polyphylla Smith var. yunnanensis( and polygerm varieties),Hg was not detected in all samples,and the rest heavy metals did not exceed the limit. [Conclusions] This study was expected to provide a certain reference for the development and utilization of P. polyphylla Smith var. yunnanensis( and polygerm varieties).
基金Supported by Key Sci-Tech Project of Sichuan Provincial Science and Technology Department(2016FZ0045)Fundamental Research Funds for the Central Universities of Southwest Minzu University(2018NZD10)
文摘[Objectives] To make original plant and microscopic identification and study the traits of Paris polyphylla Smith var. yunnanensis( Franch.) Hand.-Mazz.( polygerm varieties) produced in Gaoligong Mountain areas,and provide experimental data for their identification and application. [Methods] The original plant powder was used,and paraffin sections and physicochemical properties were identified. [Results]P. polyphylla Smith var. yunnanensis( polygerm varieties) produced in Gaoligong Mountain areas showed obvious microscopic features and physicochemical properties. [Conclusions] This study was expected to provide certain reference for identification of original plant and medicine and formulation of quality standard for P. polyphylla Smith var. yunnanensis( polygerm varieties) produced in Gaoligong Mountain areas,and provided basis for their further research and development.
文摘Coptis chinensis Franch, a widely used Traditional Chinese Medicine, shows various kinds of bioactivity. The major active components of the herb are considered to be alkaloids. Thus, preparative separation of these alkaloids is critical important for further pharmacology and mechanism studies. In the paper, five alkaloids from C. chinensis were purified by HSCCC using the solvent system composed of chloro-form-metha- nol-water (2:1:1, v/v/v) single prepared. The content of each solvent in solvent system were determined by gas chromatography (GC), then according the ratios of solvents in each phase to prepare the mobile and stationary phase respectively. And a comparative study was carried out between together preparation and single preparation of the solvent system. The purities and recoveries of all the products were over 98.5% and 92%. However, 134 mL chloroform, 336 mL methanol and 452 mL water were saved when the two phase were singled by GC. Our research showed an economical method for separating alkaloids from C. chinensis by HSCCC using the solvent system single prepared by GC.
文摘A new oleanane-type triterpene saponin. asteryunnanoside E. has been isolated from the roots of Astir yunnanensis Franch. and its structure elucidated as 3-O-β-D-glucopyranosyl-bayogenin-28-O-β-glucopyranoside by means of spectral data particularly 2D NMR including COSY. HETCOR, HOHAHA and ROESY techniques and chemical transformation.
文摘Objective:To investigate the effect of decocted Coptis chinensis Franch.(Huanglian Jianji,黄连煎剂,HLJJ) and berbenne on the gut microbiota of the rats with metabolic syndrome (MS) induced by high-fat diet (HFD).Methods:Sixty Sprague Dawley rats were randomly divided into normal diet (ND) group (n =15)and HFD group (n =45).After 8 weeks of feeding,the HFD group was further divided into three groups:a group provided with a combination of HLJJ and HFD for 4 weeks,a group provided with a combination of berberine and HFD for 4 weeks,and a group supplied only with HFD for 4 consecutive weeks.Epididymal fat weight,serum high-density lipoproteincholesterohC (HDL-C),lowdensity lipoproteincholesterol-C (LDL-C) and irisin levels were measured.In addition,the V3-V4 region of the cecum microbiota 16S rRNA gene amplicon was sequenced on the Illumina MiSeq platform.Results:MS was successfully induced in the rats with HFD for 8 weeks.After 12 weeks,serum irisin levels in the HFD + berberine group were significantly increased compared with those in the HFD group (P <.05).The high-quality tags were delineated into 1149 operational taxonomic units at a 97% similarity level.Furthermore,at the genus level,Akkermansia muciniphila (A.muciniphila),Bacteroides,and Ruminococcus were markedly increased,whereas Candidatus arthromitus,Prevotella,Phascolarctobacterium were in great decrease in the HLJJ group and the berberine group,compared with the HFD group.The abundance of A.muciniphila,Ruminococcus gnavus and Bacteroides was significantly higher in the berberine group,whereas,the abundance of Oscillospira was lower in the berberine group,compared with the HLJJ group.Conclusion:HLJJ and berberine changed the gut microbiota in rats with MS.The mechanism underlying the treatments of berberine and HLJJ on HFD-induced MS is partly different.
