The practice of conservation tillage or straw return to the farmland influences the grain yield and quality of rice(Oryza sativa).The key volatile compound responsible for the fragrance of fragrant rice is 2-acetyl-1-...The practice of conservation tillage or straw return to the farmland influences the grain yield and quality of rice(Oryza sativa).The key volatile compound responsible for the fragrance of fragrant rice is 2-acetyl-1-pyrroline(2-AP),which is significantly affected by field management measures.The purpose of this study was to investigate the impact of tillage management and straw return on the grain yield and biosynthesis of 2-AP in fragrant rice.This study was conducted over two years in 2016 and 2017 and used two fragrant rice cultivars(Meixiangzhan 2 and Xiangyaxiangzhan)as materials.The experimental design consisted of different tillage management and straw return treatments,which included three tillage management regimes:rotary tillage(T0),minimum tillage(T1),and no tillage(T2);and two straw return treatments:without straw return(S0)and straw return(S1).The straw used for the experiment was sourced from the residue of the corresponding fragrant rice cultivar harvested in the early season.Tillage management and straw return substantially affected the grain yields,grain quality,and 2-AP contents of both fragrant rice cultivars.Compared with the T0S0 treatment,tillage management and straw return resulted in 2-AP content improvements in 2016(12.41–116.85%)and 2017(34.85–103.89%)on average.Higher 2-AP contents were also detected in both fragrant rice cultivars in the T2S1 and T1S1 treatments.A structural equation model(SEM)demonstrated that the activities of enzymes related to fragrance metabolism in the leaves and grain jointly regulated the biosynthesis of precursors of fragrance metabolism in the grain,which further promoted the accumulation of 2-AP.In addition,a principal component analysis indicated that the T1S1 treatment was positively correlated with both 2-AP and grain yield.The SEM demonstrated that the enzymes related to nitrogen metabolism,parameters related to photosynthesis,and yield components contributed to the grain yield.The T1S1 treatment resulted in the highest average grain yield of 760.75 g m^(-2),which could be attributed to increases in various attributes,such as the leaf area index,SPAD value,nitrogen metabolism,panicle number m^(-2),and grain number per panicle.In summary,the minimum tillage and straw return(T1S1)treatment is more effective at simultaneously improving both the grain yield and 2-AP content in fragrant rice.展开更多
Fragrant rice has a high market value,and it is a popular rice type among consumers owing to its pleasant flavor.Plantation methods,nitrogen(N)fertilizers,and silicon(Si)fertilizers can affect the grain yield and frag...Fragrant rice has a high market value,and it is a popular rice type among consumers owing to its pleasant flavor.Plantation methods,nitrogen(N)fertilizers,and silicon(Si)fertilizers can affect the grain yield and fragrance of fragrant rice.However,the core commercial rice production attributes,namely the head rice yield(HRY)and 2-acetyl-1-pyrroline(2-AP)content of fragrant rice,under various nitrogen and silicon(N-Si)fertilization levels and different plantation methods remain unknown.The field experiment in this study was performed in the early seasons of 2018 and 2019 with two popular indica fragrant rice cultivars(Yuxiangyouzhan and Xiangyaxiangzhan).They were grown under six N-Si fertilization treatments(combinations of two levels of Si fertilizer,0 kg Si ha^(−1)(Si0)and 150 kg Si ha^(−1)(Si1),and three levels of N fertilizer,0 kg N ha^(−1)(N0),150 kg N ha^(−1)(N1),and 220 kg N ha^(−1)(N2))and three plantation methods(artificial transplanting(AT),mechanical transplanting(MT),and mechanical direct-seeding(MD)).The results showed that the N-Si fertilization treatments and all the plantation methods significantly affected the HRY and 2-AP content and related parameters of the two different fragrant rice cultivars.Compared with the Si0N0 treatment,the N-Si fertilization treatments resulted in higher HRY and 2-AP contents.The rates of brown rice,milled rice,head rice,and chalky rice of the fragrant rice also improved with the N-Si fertilization treatments.The N-Si fertilization treatments increased the activities of N metabolism enzymes and the accumulation of N and Si in various parts of the fragrant rice,and affected their antioxidant response parameters.The key parameters for the HRY and 2-AP content were assessed by redundancy analysis.Furthermore,the structural equation model revealed that the Si and N accumulation levels indirectly affected the HRY by affecting the N metabolism enzyme activity,N use efficiency,and grain quality of fragrant rice.Moreover,high N and Si accumulation directly promoted the 2-AP content or affected the antioxidant response parameters and indirectly regulated 2-AP synthesis.The interactions of the MT method with the N-Si fertilization treatments varied in the fragrant rice cultivars in terms of the HRY and 2-AP content,whereas the MD method was beneficial to the 2-AP content in both fragrant rice cultivars under the N-Si fertilization treatments.展开更多
Epoxiconazole is a triazole compound.However,the effects of epoxiconazole on crop productivity and quality were rarely reported.In this study,we investigated the effects of epoxiconazole application on yield formation...Epoxiconazole is a triazole compound.However,the effects of epoxiconazole on crop productivity and quality were rarely reported.In this study,we investigated the effects of epoxiconazole application on yield formation,grain quality attributes,and 2-acetyl-1-pyrroline(2-AP)content in fragrant rice.A three-year field experiment was carried out with a fragrant rice variety,Meixiangzhan 2.At the heading stage,0,0.02,0.04,0.08,0.16 and 0.32 g/L epoxiconazole solutions were foliar applied to fragrant rice plants,respectively.The results showed that epoxiconazole application significantly increased grain yield,seed-setting rate and 1000-grain weight.Chlorophyll content and net photosynthetic rate of fragrant rice during the grain-filling stage significantly increased due to epoxiconazole application.Foliar application of epoxiconazole at 0.08 g/L increased grain protein content and decreased both chalky rice rate and chalkiness area ratio of fragrant rice.Epoxiconazole also substantially increased grain 2-AP content by inducing the regulation in contents of related synthetic precursors,including proline,pyrroline-5-carboxylic acid,Δ1-pyrroline and methylglyoxal.Overall,foliar application of epoxiconazole could be used for the improvement in grain yield,grain quality and 2-AP content in fragrant rice production when applied concentration at 0.08-0.32 g/L.Our findings provided the new roles of epoxiconazole in crop production.展开更多
Climate conditions is an important factor affected the fragrant rice growth and development.In order to study the effects of different planting seasons on fragrant rice performance in South China,present study was con...Climate conditions is an important factor affected the fragrant rice growth and development.In order to study the effects of different planting seasons on fragrant rice performance in South China,present study was conducted with three planting seasons(early season(April to July),middle season(June to September)and late season(August to November))and three fragrant rice cultivars,‘Basmati-385’,‘Meixiangzhan-2’and‘Xiangyaxiangzhan’.The results showed that the highest grain yield and grain 2-acetyl-1-pyrroline(2-AP,key component of fragrant rice aroma)content were both recorded in late season treatment while the fragrant rice in middle season treatment produced the lowest grain yield,grain filling percentage,1000-grain weight and gain 2-AP content.The highest contents of precursors(proline,pyrroline-5-carboxylic acid and 1-pyrro-line)which related to 2-AP biosynthesis were recorded in late season treatment compared with early season treatment and middle season treatment.The highest activities of enzymes(proline dehydrogenase,pyrroline-5-carboxylic acid synthetase and ornithine transaminase)which involved in 2-AP biosynthesis were also observed in late season treatment.Moreover,the fragrant rice cultivars in late season possessed the lowest chalk rice rate,chalkiness as well as the highest brown rice rate,head rice and protein content.Thus,the optimal season for fragrant rice production in South China is the late season.展开更多
Brassinolide(BR)is a new green plant growth regulator.The present field study was conducted on two fragrant rice cultivars(i.e.,Meixiangzhan-2 and Xiangyaxiangzhan)to study the effects of foliar application of BR on f...Brassinolide(BR)is a new green plant growth regulator.The present field study was conducted on two fragrant rice cultivars(i.e.,Meixiangzhan-2 and Xiangyaxiangzhan)to study the effects of foliar application of BR on fragrant rice performance.At the heading stage,BR solutions at 0.05,0.15,0.25 and 0.50 mg L-1 were sprayed on fragrant rice at 600 liters per hectare;these treatments were named as BR1,BR2,BR3 and BR4,respectively.The treatment sprayed with distilled water was taken as a control(CK).Compared with CK,the BR2 treatment significantly enhanced the chlorophyll concentration and net photosynthetic rate for Meixiangzhan-2,and Xiangyaxiangzhan.The BR3 and BR4 treatments increased the concentrations of chlorophyll a,chlorophyll b and carotenoid,and also enhanced net photosynthetic rate by 31.91%and 40.43%,respectively.Higher grain yields were recorded in the BR2 treatment than on CK on Meixiangzhan-2,while on Xiangyaxiangzhan,the BR3 and BR4 treatments increased grain yield compared with CK.In relation to CK,higher head rice rates were recorded in the BR2 treatment for Meixiangzhan-2,and in the BR3 and BR4 treatments for Xiangyaxiangzhan.BR treatments(BR2 for Meixiangzhan-2,BR3 and BR4 for Xiangyaxiangzhan)also significantly enhanced the activities of GPX,SOD and CAT by 10.22%to 23.00%,and reduced the malonaldehyde concentration.In addition,we observed that some BR treatments(BR2 for Meixiangzhan-2,BR3 and BR4 for Xiangyaxiangzhan)decreased the grain 2-acetyl-1-pyrroline concentration of fragrant rice.展开更多
Vermicompost is an organic fertilizer contains multiple nutrient elements.However,the application of vermicompost in fragrant rice production is rarely reported.In order to study the effects of vermicompost applicatio...Vermicompost is an organic fertilizer contains multiple nutrient elements.However,the application of vermicompost in fragrant rice production is rarely reported.In order to study the effects of vermicompost application on fragrant rice(Oryza sativa L.)seedling performances,present study was conducted with two fragrant rice cultivars and four vermicompost rate treatments(0(CK),2.5(Wo1),5.0(Wo2)and 10.0(Wo3)g kg−1).The results showed that vermicompost treatments significantly increased dry weight of fragrant rice seedling by 8.31–32.56%compared with CK.21.10–59.13%higher net photosynthetic rates and 10.66–59.16%higher chlorophyll contents(chlorophyll a,chlorophyll b and total chlorophyll)were recorded in vermicompost treatments than CK.Application of vermicompost also significantly increased 2-acetyl-1-pyrroline(2-AP,the key compound of fragrant rice aroma)content and reduced the transcript level of gene BADH2 which related to 2-AP biosynthesis in fragrant rice seedling.Moreover,compared with CK,vermicompost treatments enhanced activities of superoxide dismutase,peroxidase,catalase by 24.42–28.66%,24.98–25.73%and 22.45–23.57%,respectively.11.54–40.53%lower malonaldehyde contents were recorded in vermicompost treatments in related to CK.In conclusion,vermicompost improved growth,increased 2-AP content and might enhance stress resistant of fragrant rice seedling.展开更多
Fragrant rice is popular for the good grain quality and special aroma.The present study conducted a field experiment to investigate the effects of ultrasonic seed treatment on grain yield,quality characters,physiologi...Fragrant rice is popular for the good grain quality and special aroma.The present study conducted a field experiment to investigate the effects of ultrasonic seed treatment on grain yield,quality characters,physiological properties and aroma biosynthesis of different fragrant rice genotypes.The seeds of three fragrant rice genotypes were exposed to 1 min of ultrasonic vibration and then cultivated in paddy field.The results of present study showed that ultrasonic seed treatment increased grain yield of all fragrant rice genotypes but the responses of yield formation to ultrasonic were varied with different genotypes.Compared with control,ultrasonic seed treatment increased grain 2-acetyl-1-pyrroline(2-AP,the key component of fragrant rice aroma)content by 13.40%–44.88%.Ultrasonic seed treatment also reduced the crude protein contents in grains.The head rice rate,rice length,chalky rice rate,and chalkiness degree were influenced by ultrasonic for one or two fragrant rice genotypes.The activities of peroxidase and superoxide dismutase were also enhanced due to ultrasonic seed treatment.In conclusion,ultrasonic seed treatment increased grain,regulated grain aroma and quality,and improved stress resistance of fragrant rice varieties.展开更多
In this study,we performed amplificaion and sequence analysis of exon7 of gene Badh2 of 12 fragrant rice materials,and identified the aroma of fragrant rice materials by the method of seed chewing and KOH soaking,so a...In this study,we performed amplificaion and sequence analysis of exon7 of gene Badh2 of 12 fragrant rice materials,and identified the aroma of fragrant rice materials by the method of seed chewing and KOH soaking,so as to analyze the sequence mutation of exon 7 in the Badh2 gene of rice material and its corresponding relation with the flavor character.The results showed that an 8 bp deletion(aaaa--t---ggc)and a mutation of SNP(g→t)in exon 7 of Badh2 gene were found in 10 materials,including Xiangnuo,Lvjinxiang,Meixiangzhan 2,Huaxiang,Yuexiangxuan 1,Hongyuxiang,Meixiangxuan 1,Baxiangxuan 1,Taixiangxuan 1,Taixiangxuan 2.This mutation was consistent with the mutation of EU155083 sequence in GenBank and was reported for the first time in Chinese rice materials.In these 10 fragrant rice materials with mutation,Huaxiang and Meixiangxuan 1 were identified as the heterozygote genotype,and Hongyuxiang was identified as non-fragrant rice,so the sequence mutation in exon 7 of Badh2 gene in fragrant rice materials did not correspond to aroma traits one by one;and 7 materials were identified as fragrant rice,and the brown rice of Meixiangzhan 2 and Yuexiangyuan 1 had sweet taste.The results could provide a reference for the research on the genetic mechanism of rice aroma character and the promotion of fragrant rice varieties.展开更多
Rice (<em>Oryza sativa</em> L.) is the most important cereal food grain crop and is consumed by the majority of the world’s human population. Among all cultivars, fragrant/aromatic rice is preferred by th...Rice (<em>Oryza sativa</em> L.) is the most important cereal food grain crop and is consumed by the majority of the world’s human population. Among all cultivars, fragrant/aromatic rice is preferred by the better part of the human population because of its aroma, taste and cooking quality. But most of the fragrant rice varieties are low yielding and easily sensitive to the surrounding environmental condition. Among different agronomic performances, proper nutrient management can improve the yield of fragrant rice not only by giving the required amount of nutrients but also by maintaining the health of the soil and the quality of the produce. In most cases, traditional agricultural practices degraded soil health and increased environmental pollution which leads to inferior grain quality. On the other hand, excessive application of chemical fertilizers reduced the nutrient status of the soil and badly affected the soil productivity and environmental stability. Therefore, a suitable approach of nutrient management is required to keep the production of fragrant rice to a notable amount and increase the nutrient use efficiency of soil. Application of manures and fertilizers in an appropriate dose which is the main object of nutrient management is required for its utmost importance in the growth and development of the crop that finally results in better yield and grain quality. Therefore, nutrient management is an important aspect in aromatic rice production to attain sustainable grain yield and high economic return with better quality of produce.展开更多
Rice-duck co-culture is an integrated farming technology that benefits rice production, grain quality, and ecological sustainability in paddy fields. However, little is known about the effects of rice-duck co-culture ...Rice-duck co-culture is an integrated farming technology that benefits rice production, grain quality, and ecological sustainability in paddy fields. However, little is known about the effects of rice-duck co-culture on enzyme activity involved in the biosynthesis of 2-acetyl-1-pyrroline (2-AP), the volatile that gives fragrant rice its' distinctive and sought-after aroma. The present study aimed to examine the influence of rice-duck co-culture on the photosynthesis, yield, grain quality, rice aroma, and the enzymes involved in 2-acetyl-1-pyrroline biosynthesis in the cultivar Meixiangzhan 2 during the early and late rice growing seasons of 2016 in Guangzhou, China. We compared the rice grown in paddy fields with and without ducks. We found that rice-duck co-culture not only improved the yield and quality of fragrant rice grain, but also promoted the precursors of 2-AP biosynthesis formation and 2-AP accumulation in the grain. Grain 2-AP content in rice-duck co-culture was noticeably increased with 9.60% and 20.81% in early and late seasons, respectively. Proline and pyrroline-5-carboxylic acid (P5C) (precursors of 2-AP biosynthesis) and the activity of enzymes such as proline dehydrogenase (ProDH), ornithine aminotransferase (OAT) and Δ1 pyrroline-5-carboxylic acid synthetase (P5CS) were all improved by 10.15%–12.99%, 32.91%–47.75%, 17.81%–26.71%, 6.25%–21.78%, and 10.58%–38.87% under rice-duck co-culture in both seasons, respectively. Overall, our results suggest that rice-duck co-culture is an environmentally-friendly and sustainable approach to improving rice aroma and grain quality of fragrant rice.展开更多
Dear Editor,Rice serves as a staple food for over half of the global population,with fragrant varieties particularly valuable for their distinctive aroma,superior taste,and favorable cooking qualities.These attributes...Dear Editor,Rice serves as a staple food for over half of the global population,with fragrant varieties particularly valuable for their distinctive aroma,superior taste,and favorable cooking qualities.These attributes have been methodically perpetuated through generations via selective breeding(Prodhan and Qingyao,2020).The aroma quality of rice is predominantly determined by the concentration of 2-acetyl-1-pyrroline(2-AP),the key compound responsible for its characteristic fragrance(Okpala et al.,2019).The identification of the first aroma-causing gene in rice,BADH2,marked a critical advance in elucidating the genetic basis of rice aroma.This breakthrough was attributed to the discovery of a specific 8-bp deletion in the BADH2 gene on chromosome 8,which is closely associated with the aromatic trait across diverse rice varieties(Bradbury et al.,2005).The targeted genetic manipulation of the BADH2 gene via genetic engineering and genome-editing techniques has facilitated the development of multiple aromatic rice varieties(Shan et al.,2015;Tang et al.,2021;Hui et al.,2022).Nevertheless,the observed variability in 2-AP concentrations across different fragrant rice varieties,including those with a functional OsBADH2 gene,highlights the complexity of the genetic mechanisms that govern rice aroma.This suggests that,in addition to OsBADH2,other genetic alleles might also contribute to the synthesis of this key aromatic compound(Huang et al.,2008).展开更多
One of Cambodia’s leading rice exporters,Apsara Rice,is known for its flagship product Apsara fragrant rice.Nurtured by the country’s fertile red soil and ample sunlight and rainfall thanks to its tropical monsoon c...One of Cambodia’s leading rice exporters,Apsara Rice,is known for its flagship product Apsara fragrant rice.Nurtured by the country’s fertile red soil and ample sunlight and rainfall thanks to its tropical monsoon climate,this rice features plump,translucent grains with a rich aroma and a soft,sticky,and elastic texture.展开更多
Yunjing 29 bred by Institute of Food Crops of Yunnan Academy of Agricultural Sciences is a new conventional japonica rice variety with fragrant and sof rice, it was examined and approved by Yunnan Provincial Variety E...Yunjing 29 bred by Institute of Food Crops of Yunnan Academy of Agricultural Sciences is a new conventional japonica rice variety with fragrant and sof rice, it was examined and approved by Yunnan Provincial Variety Examination and Approval Committee in March of 2011. The variety is early-maturing, high-yield and good-quality, and has resistances to lodging, blast and bacterial leaf blight, its rice is sweet soft, goluptious and lucidus as well as not coarse when it is cold, so it is a good commodity. To further promote the popularization and cultivation of the variety, maintain its characteristics of high quality and high yield, and prevent commingling and degeneration, the purification and rejuvenation as well as breeding technology of high-quality seeds were proposed after continuous exploration and study.展开更多
Taoxiangyoulijing is a new fine quality fragrant hybrid combination bred by matching the fine quality indica sterile line Taoxiang A with the fine quality restorer line R Lijing by Hunan Taohuayuan Agricultural Scienc...Taoxiangyoulijing is a new fine quality fragrant hybrid combination bred by matching the fine quality indica sterile line Taoxiang A with the fine quality restorer line R Lijing by Hunan Taohuayuan Agricultural Science and Technology Co.Ltd,Changsha Zhulixiang Agricultural Science and Technology Co.Ltd and Hunan Zhihe Seed Technology Co.,Ltd.The combination is featured with wide adaptability,high yield,strong resistance to rice blast,and GB class 2 high quality.In October 2019,Taoxiangyoulijing was approved by the National Crop Variety Certification Committee and it reached the second class standard of quality score according to the Standard for Fine Quality Rice,Ministry of Agriculture,China.展开更多
基金funding provided by the National Natural Science Foundation of China(31971843 and 31271646)the Guangxi Natural Science Foundation,China(2021GXNSFBA196084)。
文摘The practice of conservation tillage or straw return to the farmland influences the grain yield and quality of rice(Oryza sativa).The key volatile compound responsible for the fragrance of fragrant rice is 2-acetyl-1-pyrroline(2-AP),which is significantly affected by field management measures.The purpose of this study was to investigate the impact of tillage management and straw return on the grain yield and biosynthesis of 2-AP in fragrant rice.This study was conducted over two years in 2016 and 2017 and used two fragrant rice cultivars(Meixiangzhan 2 and Xiangyaxiangzhan)as materials.The experimental design consisted of different tillage management and straw return treatments,which included three tillage management regimes:rotary tillage(T0),minimum tillage(T1),and no tillage(T2);and two straw return treatments:without straw return(S0)and straw return(S1).The straw used for the experiment was sourced from the residue of the corresponding fragrant rice cultivar harvested in the early season.Tillage management and straw return substantially affected the grain yields,grain quality,and 2-AP contents of both fragrant rice cultivars.Compared with the T0S0 treatment,tillage management and straw return resulted in 2-AP content improvements in 2016(12.41–116.85%)and 2017(34.85–103.89%)on average.Higher 2-AP contents were also detected in both fragrant rice cultivars in the T2S1 and T1S1 treatments.A structural equation model(SEM)demonstrated that the activities of enzymes related to fragrance metabolism in the leaves and grain jointly regulated the biosynthesis of precursors of fragrance metabolism in the grain,which further promoted the accumulation of 2-AP.In addition,a principal component analysis indicated that the T1S1 treatment was positively correlated with both 2-AP and grain yield.The SEM demonstrated that the enzymes related to nitrogen metabolism,parameters related to photosynthesis,and yield components contributed to the grain yield.The T1S1 treatment resulted in the highest average grain yield of 760.75 g m^(-2),which could be attributed to increases in various attributes,such as the leaf area index,SPAD value,nitrogen metabolism,panicle number m^(-2),and grain number per panicle.In summary,the minimum tillage and straw return(T1S1)treatment is more effective at simultaneously improving both the grain yield and 2-AP content in fragrant rice.
基金This project was financially supported by the National Natural Science Foundation of China(31601244 and 31971843)the Guangdong Provincial Key Field Research and Development Plan Project,China(2019B020221003)the Modern Agricultural Industrial Technology System of Guangdong Province,China(2020KJ105).
文摘Fragrant rice has a high market value,and it is a popular rice type among consumers owing to its pleasant flavor.Plantation methods,nitrogen(N)fertilizers,and silicon(Si)fertilizers can affect the grain yield and fragrance of fragrant rice.However,the core commercial rice production attributes,namely the head rice yield(HRY)and 2-acetyl-1-pyrroline(2-AP)content of fragrant rice,under various nitrogen and silicon(N-Si)fertilization levels and different plantation methods remain unknown.The field experiment in this study was performed in the early seasons of 2018 and 2019 with two popular indica fragrant rice cultivars(Yuxiangyouzhan and Xiangyaxiangzhan).They were grown under six N-Si fertilization treatments(combinations of two levels of Si fertilizer,0 kg Si ha^(−1)(Si0)and 150 kg Si ha^(−1)(Si1),and three levels of N fertilizer,0 kg N ha^(−1)(N0),150 kg N ha^(−1)(N1),and 220 kg N ha^(−1)(N2))and three plantation methods(artificial transplanting(AT),mechanical transplanting(MT),and mechanical direct-seeding(MD)).The results showed that the N-Si fertilization treatments and all the plantation methods significantly affected the HRY and 2-AP content and related parameters of the two different fragrant rice cultivars.Compared with the Si0N0 treatment,the N-Si fertilization treatments resulted in higher HRY and 2-AP contents.The rates of brown rice,milled rice,head rice,and chalky rice of the fragrant rice also improved with the N-Si fertilization treatments.The N-Si fertilization treatments increased the activities of N metabolism enzymes and the accumulation of N and Si in various parts of the fragrant rice,and affected their antioxidant response parameters.The key parameters for the HRY and 2-AP content were assessed by redundancy analysis.Furthermore,the structural equation model revealed that the Si and N accumulation levels indirectly affected the HRY by affecting the N metabolism enzyme activity,N use efficiency,and grain quality of fragrant rice.Moreover,high N and Si accumulation directly promoted the 2-AP content or affected the antioxidant response parameters and indirectly regulated 2-AP synthesis.The interactions of the MT method with the N-Si fertilization treatments varied in the fragrant rice cultivars in terms of the HRY and 2-AP content,whereas the MD method was beneficial to the 2-AP content in both fragrant rice cultivars under the N-Si fertilization treatments.
基金This study was supported by the National Natural Science Foundation of China(Grant No.31971843)the Technology System of Modern Agricultural Industry in Guangdong(Grant No.2020KJ105)Guangzhou Science and Technology Project in China(Grant No.202103000075).
文摘Epoxiconazole is a triazole compound.However,the effects of epoxiconazole on crop productivity and quality were rarely reported.In this study,we investigated the effects of epoxiconazole application on yield formation,grain quality attributes,and 2-acetyl-1-pyrroline(2-AP)content in fragrant rice.A three-year field experiment was carried out with a fragrant rice variety,Meixiangzhan 2.At the heading stage,0,0.02,0.04,0.08,0.16 and 0.32 g/L epoxiconazole solutions were foliar applied to fragrant rice plants,respectively.The results showed that epoxiconazole application significantly increased grain yield,seed-setting rate and 1000-grain weight.Chlorophyll content and net photosynthetic rate of fragrant rice during the grain-filling stage significantly increased due to epoxiconazole application.Foliar application of epoxiconazole at 0.08 g/L increased grain protein content and decreased both chalky rice rate and chalkiness area ratio of fragrant rice.Epoxiconazole also substantially increased grain 2-AP content by inducing the regulation in contents of related synthetic precursors,including proline,pyrroline-5-carboxylic acid,Δ1-pyrroline and methylglyoxal.Overall,foliar application of epoxiconazole could be used for the improvement in grain yield,grain quality and 2-AP content in fragrant rice production when applied concentration at 0.08-0.32 g/L.Our findings provided the new roles of epoxiconazole in crop production.
基金This work was supported by National Natural Science Foundation of China(31271646)Agricultural and Rural Department Foundation of Guangdong Province(2018kczx06)+1 种基金Special fund for scientific innovation strategy-construction of high-level Academy of Agriculture Science(R2019QD-002)Key Areas Research Projects of Guangdong Province(2020B020225004)and(2018B020202004).
文摘Climate conditions is an important factor affected the fragrant rice growth and development.In order to study the effects of different planting seasons on fragrant rice performance in South China,present study was conducted with three planting seasons(early season(April to July),middle season(June to September)and late season(August to November))and three fragrant rice cultivars,‘Basmati-385’,‘Meixiangzhan-2’and‘Xiangyaxiangzhan’.The results showed that the highest grain yield and grain 2-acetyl-1-pyrroline(2-AP,key component of fragrant rice aroma)content were both recorded in late season treatment while the fragrant rice in middle season treatment produced the lowest grain yield,grain filling percentage,1000-grain weight and gain 2-AP content.The highest contents of precursors(proline,pyrroline-5-carboxylic acid and 1-pyrro-line)which related to 2-AP biosynthesis were recorded in late season treatment compared with early season treatment and middle season treatment.The highest activities of enzymes(proline dehydrogenase,pyrroline-5-carboxylic acid synthetase and ornithine transaminase)which involved in 2-AP biosynthesis were also observed in late season treatment.Moreover,the fragrant rice cultivars in late season possessed the lowest chalk rice rate,chalkiness as well as the highest brown rice rate,head rice and protein content.Thus,the optimal season for fragrant rice production in South China is the late season.
基金This study was financially supported by National Natural Science Foundation of China(31971843)Technology System of Modern Agricultural Industry in Guangdong(2020KJ105)Guangzhou Science and Technology Project(202103000075).
文摘Brassinolide(BR)is a new green plant growth regulator.The present field study was conducted on two fragrant rice cultivars(i.e.,Meixiangzhan-2 and Xiangyaxiangzhan)to study the effects of foliar application of BR on fragrant rice performance.At the heading stage,BR solutions at 0.05,0.15,0.25 and 0.50 mg L-1 were sprayed on fragrant rice at 600 liters per hectare;these treatments were named as BR1,BR2,BR3 and BR4,respectively.The treatment sprayed with distilled water was taken as a control(CK).Compared with CK,the BR2 treatment significantly enhanced the chlorophyll concentration and net photosynthetic rate for Meixiangzhan-2,and Xiangyaxiangzhan.The BR3 and BR4 treatments increased the concentrations of chlorophyll a,chlorophyll b and carotenoid,and also enhanced net photosynthetic rate by 31.91%and 40.43%,respectively.Higher grain yields were recorded in the BR2 treatment than on CK on Meixiangzhan-2,while on Xiangyaxiangzhan,the BR3 and BR4 treatments increased grain yield compared with CK.In relation to CK,higher head rice rates were recorded in the BR2 treatment for Meixiangzhan-2,and in the BR3 and BR4 treatments for Xiangyaxiangzhan.BR treatments(BR2 for Meixiangzhan-2,BR3 and BR4 for Xiangyaxiangzhan)also significantly enhanced the activities of GPX,SOD and CAT by 10.22%to 23.00%,and reduced the malonaldehyde concentration.In addition,we observed that some BR treatments(BR2 for Meixiangzhan-2,BR3 and BR4 for Xiangyaxiangzhan)decreased the grain 2-acetyl-1-pyrroline concentration of fragrant rice.
基金supported by National Natural Science Foundation of China(31971843)Technology System of Modern Agricultural Industry in Guangdong(2019KJ105)XT received the grant.
文摘Vermicompost is an organic fertilizer contains multiple nutrient elements.However,the application of vermicompost in fragrant rice production is rarely reported.In order to study the effects of vermicompost application on fragrant rice(Oryza sativa L.)seedling performances,present study was conducted with two fragrant rice cultivars and four vermicompost rate treatments(0(CK),2.5(Wo1),5.0(Wo2)and 10.0(Wo3)g kg−1).The results showed that vermicompost treatments significantly increased dry weight of fragrant rice seedling by 8.31–32.56%compared with CK.21.10–59.13%higher net photosynthetic rates and 10.66–59.16%higher chlorophyll contents(chlorophyll a,chlorophyll b and total chlorophyll)were recorded in vermicompost treatments than CK.Application of vermicompost also significantly increased 2-acetyl-1-pyrroline(2-AP,the key compound of fragrant rice aroma)content and reduced the transcript level of gene BADH2 which related to 2-AP biosynthesis in fragrant rice seedling.Moreover,compared with CK,vermicompost treatments enhanced activities of superoxide dismutase,peroxidase,catalase by 24.42–28.66%,24.98–25.73%and 22.45–23.57%,respectively.11.54–40.53%lower malonaldehyde contents were recorded in vermicompost treatments in related to CK.In conclusion,vermicompost improved growth,increased 2-AP content and might enhance stress resistant of fragrant rice seedling.
基金supported by National Natural Science Foundation of China(31971843)The Technology System of Modern Agricultural Industry in Guangdong(2020KJ105)Guangzhou Science and Technology Project(202103000075).Xiangru Tang received the grants.
文摘Fragrant rice is popular for the good grain quality and special aroma.The present study conducted a field experiment to investigate the effects of ultrasonic seed treatment on grain yield,quality characters,physiological properties and aroma biosynthesis of different fragrant rice genotypes.The seeds of three fragrant rice genotypes were exposed to 1 min of ultrasonic vibration and then cultivated in paddy field.The results of present study showed that ultrasonic seed treatment increased grain yield of all fragrant rice genotypes but the responses of yield formation to ultrasonic were varied with different genotypes.Compared with control,ultrasonic seed treatment increased grain 2-acetyl-1-pyrroline(2-AP,the key component of fragrant rice aroma)content by 13.40%–44.88%.Ultrasonic seed treatment also reduced the crude protein contents in grains.The head rice rate,rice length,chalky rice rate,and chalkiness degree were influenced by ultrasonic for one or two fragrant rice genotypes.The activities of peroxidase and superoxide dismutase were also enhanced due to ultrasonic seed treatment.In conclusion,ultrasonic seed treatment increased grain,regulated grain aroma and quality,and improved stress resistance of fragrant rice varieties.
基金Earmarked Fund for China Agriculture Research System(CARS-01-89)Fundamental Scientific Research Fund of Hainan Academy of Agricultural Sciences(JBKYYWF2020-03)Science and Technology Innovation Project of Hainan Academy of Agricultural Sciences(KJCX-2020-11)。
文摘In this study,we performed amplificaion and sequence analysis of exon7 of gene Badh2 of 12 fragrant rice materials,and identified the aroma of fragrant rice materials by the method of seed chewing and KOH soaking,so as to analyze the sequence mutation of exon 7 in the Badh2 gene of rice material and its corresponding relation with the flavor character.The results showed that an 8 bp deletion(aaaa--t---ggc)and a mutation of SNP(g→t)in exon 7 of Badh2 gene were found in 10 materials,including Xiangnuo,Lvjinxiang,Meixiangzhan 2,Huaxiang,Yuexiangxuan 1,Hongyuxiang,Meixiangxuan 1,Baxiangxuan 1,Taixiangxuan 1,Taixiangxuan 2.This mutation was consistent with the mutation of EU155083 sequence in GenBank and was reported for the first time in Chinese rice materials.In these 10 fragrant rice materials with mutation,Huaxiang and Meixiangxuan 1 were identified as the heterozygote genotype,and Hongyuxiang was identified as non-fragrant rice,so the sequence mutation in exon 7 of Badh2 gene in fragrant rice materials did not correspond to aroma traits one by one;and 7 materials were identified as fragrant rice,and the brown rice of Meixiangzhan 2 and Yuexiangyuan 1 had sweet taste.The results could provide a reference for the research on the genetic mechanism of rice aroma character and the promotion of fragrant rice varieties.
文摘Rice (<em>Oryza sativa</em> L.) is the most important cereal food grain crop and is consumed by the majority of the world’s human population. Among all cultivars, fragrant/aromatic rice is preferred by the better part of the human population because of its aroma, taste and cooking quality. But most of the fragrant rice varieties are low yielding and easily sensitive to the surrounding environmental condition. Among different agronomic performances, proper nutrient management can improve the yield of fragrant rice not only by giving the required amount of nutrients but also by maintaining the health of the soil and the quality of the produce. In most cases, traditional agricultural practices degraded soil health and increased environmental pollution which leads to inferior grain quality. On the other hand, excessive application of chemical fertilizers reduced the nutrient status of the soil and badly affected the soil productivity and environmental stability. Therefore, a suitable approach of nutrient management is required to keep the production of fragrant rice to a notable amount and increase the nutrient use efficiency of soil. Application of manures and fertilizers in an appropriate dose which is the main object of nutrient management is required for its utmost importance in the growth and development of the crop that finally results in better yield and grain quality. Therefore, nutrient management is an important aspect in aromatic rice production to attain sustainable grain yield and high economic return with better quality of produce.
基金supported by the Science and Technology Project of Guangdong Province (2015B090903077, 2016A020210094, 2017A090905030), Chinathe Science and Technology Project of Guangzhou (201604020062), China+1 种基金the Innovation Team Construction Project of Modern Agricultural Industry Technology System of Guangdong Province (2016LM1100), Chinathe Overseas Joint Doctoral Training Program of South China Agricultural University (2018LHPY010), China
文摘Rice-duck co-culture is an integrated farming technology that benefits rice production, grain quality, and ecological sustainability in paddy fields. However, little is known about the effects of rice-duck co-culture on enzyme activity involved in the biosynthesis of 2-acetyl-1-pyrroline (2-AP), the volatile that gives fragrant rice its' distinctive and sought-after aroma. The present study aimed to examine the influence of rice-duck co-culture on the photosynthesis, yield, grain quality, rice aroma, and the enzymes involved in 2-acetyl-1-pyrroline biosynthesis in the cultivar Meixiangzhan 2 during the early and late rice growing seasons of 2016 in Guangzhou, China. We compared the rice grown in paddy fields with and without ducks. We found that rice-duck co-culture not only improved the yield and quality of fragrant rice grain, but also promoted the precursors of 2-AP biosynthesis formation and 2-AP accumulation in the grain. Grain 2-AP content in rice-duck co-culture was noticeably increased with 9.60% and 20.81% in early and late seasons, respectively. Proline and pyrroline-5-carboxylic acid (P5C) (precursors of 2-AP biosynthesis) and the activity of enzymes such as proline dehydrogenase (ProDH), ornithine aminotransferase (OAT) and Δ1 pyrroline-5-carboxylic acid synthetase (P5CS) were all improved by 10.15%–12.99%, 32.91%–47.75%, 17.81%–26.71%, 6.25%–21.78%, and 10.58%–38.87% under rice-duck co-culture in both seasons, respectively. Overall, our results suggest that rice-duck co-culture is an environmentally-friendly and sustainable approach to improving rice aroma and grain quality of fragrant rice.
基金supported by the Project of Zhong Shan Laboratory of Biological Breeding(BM2022008-02 and ZSBBL-KY2023-06)the Jiangsu Agricultural Science and Technology Innovation Fund(CX(21)1002)+1 种基金the Jiangsu Key Research and Development Program(BE2022365)the National Natural Science Foundation of China(3217047).
文摘Dear Editor,Rice serves as a staple food for over half of the global population,with fragrant varieties particularly valuable for their distinctive aroma,superior taste,and favorable cooking qualities.These attributes have been methodically perpetuated through generations via selective breeding(Prodhan and Qingyao,2020).The aroma quality of rice is predominantly determined by the concentration of 2-acetyl-1-pyrroline(2-AP),the key compound responsible for its characteristic fragrance(Okpala et al.,2019).The identification of the first aroma-causing gene in rice,BADH2,marked a critical advance in elucidating the genetic basis of rice aroma.This breakthrough was attributed to the discovery of a specific 8-bp deletion in the BADH2 gene on chromosome 8,which is closely associated with the aromatic trait across diverse rice varieties(Bradbury et al.,2005).The targeted genetic manipulation of the BADH2 gene via genetic engineering and genome-editing techniques has facilitated the development of multiple aromatic rice varieties(Shan et al.,2015;Tang et al.,2021;Hui et al.,2022).Nevertheless,the observed variability in 2-AP concentrations across different fragrant rice varieties,including those with a functional OsBADH2 gene,highlights the complexity of the genetic mechanisms that govern rice aroma.This suggests that,in addition to OsBADH2,other genetic alleles might also contribute to the synthesis of this key aromatic compound(Huang et al.,2008).
文摘One of Cambodia’s leading rice exporters,Apsara Rice,is known for its flagship product Apsara fragrant rice.Nurtured by the country’s fertile red soil and ample sunlight and rainfall thanks to its tropical monsoon climate,this rice features plump,translucent grains with a rich aroma and a soft,sticky,and elastic texture.
基金Supported by the Project of Technology Innovation and Talent Cultivation in Yunnan Province(2015HB107)the Project of New Product Development of Yunnan Province"The Breeding of New Conventional Rice Variety and Its Industrialization Development"(2012BB013)+3 种基金the Major Special Project of Biological Seed Industry in Yunnan Province"The ResearchApplication of the Key Technology of the Industrialization of Plateau Japonica Rice Seed Industry"(2015ZA003)the Project of"Leading Talents Training of Yunling Industrial Technology"in Yunnan Provincethe Project of Rice Industrial Technology System of Modern Agriculture in Yunnan Province~~
文摘Yunjing 29 bred by Institute of Food Crops of Yunnan Academy of Agricultural Sciences is a new conventional japonica rice variety with fragrant and sof rice, it was examined and approved by Yunnan Provincial Variety Examination and Approval Committee in March of 2011. The variety is early-maturing, high-yield and good-quality, and has resistances to lodging, blast and bacterial leaf blight, its rice is sweet soft, goluptious and lucidus as well as not coarse when it is cold, so it is a good commodity. To further promote the popularization and cultivation of the variety, maintain its characteristics of high quality and high yield, and prevent commingling and degeneration, the purification and rejuvenation as well as breeding technology of high-quality seeds were proposed after continuous exploration and study.
文摘Taoxiangyoulijing is a new fine quality fragrant hybrid combination bred by matching the fine quality indica sterile line Taoxiang A with the fine quality restorer line R Lijing by Hunan Taohuayuan Agricultural Science and Technology Co.Ltd,Changsha Zhulixiang Agricultural Science and Technology Co.Ltd and Hunan Zhihe Seed Technology Co.,Ltd.The combination is featured with wide adaptability,high yield,strong resistance to rice blast,and GB class 2 high quality.In October 2019,Taoxiangyoulijing was approved by the National Crop Variety Certification Committee and it reached the second class standard of quality score according to the Standard for Fine Quality Rice,Ministry of Agriculture,China.
