The SafeAmpCase is an innovative 3D-printed solution developed to address critical challenges in transporting and storing fragile glass drug ampoules during emergencies.This study employs a multidisciplinary approach...The SafeAmpCase is an innovative 3D-printed solution developed to address critical challenges in transporting and storing fragile glass drug ampoules during emergencies.This study employs a multidisciplinary approach—integrating biomedical engineering,advanced materials science,and emergency medicine expertise—to develop a compact,durable,and user-friendly ampoule case.A key innovation lies in the strategic selection of thermoplastic polyurethane(TPU)as the material,leveraging its superior impact resistance,flexibility,and noise-damping characteristics to ensure reliability under performance in demanding real-world conditions.To optimize the 3D printing process,key parameters,including printing temperature(220-250℃),volumetric flow rate(3-20 mm^(3)/s),retraction speed(30-90 mm/s),and retraction length(0.4-1.2 mm),were systematically adjusted using calibration models.The final optimized parameters(245℃,7 mm^(3)/s,90 mm/s,and 1.2 mm)reduced production time by 43%while preserving structural integrity.American Society for Testing and Materials(ASTM)international standard drop tests confirmed the case’s exceptional impact resistance,demonstrating a 90%reduction in ampoule breakage compared to polylactic acid plus.Further refinements,guided by feedback from 25 emergency professionals,resulted in medicationspecific color coding and an enhanced locking mechanism for usability in high-pressure situations.The final SafeAmpCase model withstood 18 consecutive drop trials without ampoule breakage,confirming its robustness in field conditions.This research underscores the transformative potential of additive manufacturing in developing customized,high-performance solutions for critical healthcare applications,setting a new benchmark for biomedical device design and rapid prototyping.展开更多
Biological invasions have become recognized as one of the greatest threats to ecosystems.Codium,a genus of invasive green algae,has frequent global outbreaks and damages local marine ecosystems.It is now generally acc...Biological invasions have become recognized as one of the greatest threats to ecosystems.Codium,a genus of invasive green algae,has frequent global outbreaks and damages local marine ecosystems.It is now generally accepted that light is one of the main factors affecting the luxuriant growth of macroalgae such as Codium.In this study,to investigate the invasive photo-adaptation strategy of Codium fragile,the photo-adaptation characteristics of C.fragile and Codium.cylindricum from the Nan’ao Island of China were compared and explored.The effect of light intensity on the photosynthetic properties of the two species was investigated:the maximum quantum yield of photosystemⅡ(Fv/Fm)of C.fragile was significantly higher at low light intensity.At a light intensity of 90μmol/(m^(2)·s),maximum relative electron transport rate(rETR_(max))of the thalli was maximum,and the minimum saturating irradiance(Ek)was significantly increased.The photosynthetic rate(αvalue)of thalli was highest at a light intensity of 30μmol/(m^(2)·s).The photochemical quenching(qP)was enhanced but non-photochemical quenching(NPQ)was reduced at high light intensities.As for C.cylindricum,the optimal photochemical efficiency of the thalli at low light intensity was higher.High light intensity significantly reduced the rETR of the thalli.At low light intensity,αwas significantly higher,Ek was significantly lower,and NPQ was also significantly decreased.The response relationship between light acclimation and antioxidant capacity of the thalli of two species of Codium was investigated:there was no significant effect of light intensity variation on the total antioxidant capacity of C.fragile.In the case of C.cylindricum,the degree of membrane lipid peroxidation was significantly increased at low light intensity,and its antioxidant capacity was significantly reduced when the light intensity was too high or too low.It can be hypothesized that the self-protection ability of C.fragile may be stronger than that of C.cylindricum under low and high light intensities,which is closely related to the strong invasiveness of C.fragile.展开更多
Biometric template protection is essential for finger-based authentication systems,as template tampering and adversarial attacks threaten the security.This paper proposes a DCT-based fragile watermarking scheme incorp...Biometric template protection is essential for finger-based authentication systems,as template tampering and adversarial attacks threaten the security.This paper proposes a DCT-based fragile watermarking scheme incorporating AI-based tamper detection to improve the integrity and robustness of finger authentication.The system was tested against NIST SD4 and Anguli fingerprint datasets,wherein 10,000 watermarked fingerprints were employed for training.The designed approach recorded a tamper detection rate of 98.3%,performing 3–6%better than current DCT,SVD,and DWT-based watermarking approaches.The false positive rate(≤1.2%)and false negative rate(≤1.5%)were much lower compared to previous research,which maintained high reliability for template change detection.The system showed real-time performance,averaging 12–18 ms processing time per template,and is thus suitable for real-world biometric authentication scenarios.Quality analysis of fingerprints indicated that NFIQ scores were enhanced from 2.07 to 1.81,reflecting improved minutiae clarity and ridge structure preservation.The approach also exhibited strong resistance to compression and noise distortions,with the improvements in PSNR being 2 dB(JPEG compression Q=80)and the SSIM values rising by 3%–5%under noise attacks.Comparative assessment demonstrated that training with NIST SD4 data greatly improved the ridge continuity and quality of fingerprints,resulting in better match scores(260–295)when tested against Bozorth3.Smaller batch sizes(batch=2)also resulted in improved ridge clarity,whereas larger batch sizes(batch=8)resulted in distortions.The DCNN-based tamper detection model supported real-time classification,which greatly minimized template exposure to adversarial attacks and synthetic fingerprint forgeries.Results demonstrate that fragile watermarking with AI indeed greatly enhances fingerprint security,providing privacy-preserving biometric authentication with high robustness,accuracy,and computational efficiency.展开更多
Guazhou County of Gansu Province is a typical ecological fragile area lying in the transition area of desert and oasis.The development of its agricultural economy is highly dependent on natural resources and environme...Guazhou County of Gansu Province is a typical ecological fragile area lying in the transition area of desert and oasis.The development of its agricultural economy is highly dependent on natural resources and environment.Contradiction of resources characterized agriculture and the fragile ecological environment has become one of the main barriers to log the impact of local agriculture to achieve sustainable development.Aiming at fragile ecological environment and limiting factors of agricultural sustainable development,thoughts and countermeasures of realizing agricultural sustainable development have been proposed.展开更多
[Objective] The aim was to explore eco-economy in ecological fragile region with Wuqi County in Shaanxi Province as an example. [Method] Eco-economy development and major environmental problems in Wuqi County in Shaan...[Objective] The aim was to explore eco-economy in ecological fragile region with Wuqi County in Shaanxi Province as an example. [Method] Eco-economy development and major environmental problems in Wuqi County in Shaanxi Province were researched based on local social and economic conditions, population of impoverished people and environment vulnerability. [Result] The areas with vulnerable environment and backward economy should establish a virtuous circle between economy and environment and a sustainable model of economic development. [Conclusion] It is significant to change the severe situation of environment deterioration and to promote ecological civilization.展开更多
Peptide composition and arrangement of 4 major light-harvesting complexes LHCP 1-3 and LHCP 3′ isolated from siphonous green algae (Codium fragile (Sur.) Hariot.) were investigated. LHCP 1 showed five main pep...Peptide composition and arrangement of 4 major light-harvesting complexes LHCP 1-3 and LHCP 3′ isolated from siphonous green algae (Codium fragile (Sur.) Hariot.) were investigated. LHCP 1 showed five main peptides, 34.4, 31.5, 29.5, 28.2 and 26.5 kD in SDS-PAGE, the 34.4 and 31.5 kD peptides were never found in higher plants. LHCP 3 contained the other four kinds of LHCP 1 peptides except 34.4 kD, while LHCP 3′ consisted of only 28.2 and 26.5 kD peptides. We found that 34.4, 28.2 and 26.5 kD peptides were easy to decompose from LHCP 1 when subjected to SDS-PAGE without pretreatment. They might be located at the exterior of LHCP 1, while the 31.5 and 29.5 kD peptides were at the central part. The 28.2 and 26.5 kD peptides often occurred in CPa, the center complex of PSⅡ. They are possibly the LHCⅡ peptides tightly associated with CCⅡ. According to the results described above, a peptide map of LHCP 1 was sketched.展开更多
AIM: To evaluate the expression of fragile histidine triad (FHIT) gene protein, product of a candidate tumor suppressor, and to investigate the relationship between FHIT, cell apoptosis and proliferation, and patholog...AIM: To evaluate the expression of fragile histidine triad (FHIT) gene protein, product of a candidate tumor suppressor, and to investigate the relationship between FHIT, cell apoptosis and proliferation, and pathological features of primary hepatocellular carcinoma (HCC). METHODS: Forty-seven HCC and ten normal liver specimens were collected during surgical operation between 2001 and 2003. FHIT and proliferating cell nuclear antigen (PCNA) expression were detected by immunohistochemistry, and apoptotic level was evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay on the tissue sections. RESULTS: All normal liver tissues showed a strong expression of FHIT, whereas 28 of 47 (59.6%) carcinomas showed a significant loss or absence of FHIT expression (P= 0.001). The proportion of reduced FHIT expression in those carcinomas at stages Ⅲ-Ⅳ (70.6%) and in those with extrahepatic metastasis (86.7%) showed an increasing trend compared with those at stages HI (30.8%, P= 0.013) and those without metastasis (46.9%, P = 0.010) respectively. Apoptotic incidence in advanced TNM stage carcinoma and those with positive FHIT expression was higher than that in early stage carcinoma (P=0.030) and in those with negative FHIT expression (P=0.044) respectively. The proliferating potential of hepatocellular carcinoma was associated with FHIT expression (P= 0.016) and the aggressive feature (P = 0.019). Kaplan-Meier analysis demonstrated that the survival time of these 47 patients correlated with TNM stage, FHIT expression and metastasis. CONCLUSION: There is marked loss or absence of FHIT expression, as well as abnormal apoptosis-prdiferation balance in HCC. FHIT may play an important role in carcinogenesis and development of HCC.展开更多
Fragile X syndrome （FXS） is one of the most prevalent mental retardations. It is mainly caused by the loss of fragile X mental retardation protein （FMRP）. FMRP is an RNA binding protein and can regulate the transl...Fragile X syndrome （FXS） is one of the most prevalent mental retardations. It is mainly caused by the loss of fragile X mental retardation protein （FMRP）. FMRP is an RNA binding protein and can regulate the translation of its binding RNA, thus regulate several signaling pathways. Many FXS patients show high susceptibility to epilepsy. Epilepsy is a chronic neurological disorder which is characterized by the recurrent appearance of spontaneous seizures due to neuronal hyperactivity in the brain. Both the abnormal activation of several signaling pathway and morphological abnormality that are caused by the loss of FMRP can lead to a high susceptibility to epilepsy. Combining with the research progresses on both FXS and epilepsy, we outlined the possible mechanisms of high susceptibility to epilepsy in FXS and tried to give a prospect on the future research on the mechanism of epilepsy that happened in other mental retardations.展开更多
AIM： To detect the expression of fragile histidine triad （FHIT） in normal colorectal tissue, colorectal adenoma and colorectal cancer （CRC） tissue, and to analyze its relationship with the clinicopathological fea...AIM： To detect the expression of fragile histidine triad （FHIT） in normal colorectal tissue, colorectal adenoma and colorectal cancer （CRC） tissue, and to analyze its relationship with the clinicopathological features of CRC, and apoptosis-associatecl proteins （Bcl-2, Bax, survivin） and apoptosis in colorectal cancer. METHODS： FHIT mRNA analysis was performed by nested reverse transcription-polymerase chain reaction （RT-PCR） assay. Tissue microarray （TMA） was established to detect the expression of FHIT, Bcl-2, Bax and survivin genes in 80 CRC tissue specimens, 16 colorectal adenoma tissue specimens and 16 hemorrhoid （PPH） tissue specimens during the same period of time as the control. Citrate-microwave-SP was used as immunohistochemical method. The relationship between clinicopathological factors, such as differentiation grades and 5-year survival rate was observed. TUNEL assay was used to detect the apoptosis index in 80 CRC tissue specimens. RESULTS： Ten out of 26 （38.5%） CRC tissue specimens expressed aberrant FHIT transcripts, none of the aberrant FHIT transcripts was observed in the matchednormal tissue and colorectal adenoma tissue by nested RT-PCR assay. The positive rate of FHIT gene expression in normal colorectal tissue, colorectal adenoma and carcinoma tissue was 93.75%, 68.75% and 46.25%, respectively. Clinicopathological analysis of patients showed that the decreased FHIT gene expression was not associated with age, sex, serum CEA levels, tumor site and size, histological classification. However, the expression of FHIT was correlated with differentiation grades, pathological stages, lymph node metastases and 5-year survival rate after operation. The positive rate of apoptosis-associated proteins （Bax, Bcl-2 and survivin） in CRC tissue was 72.50%, 51.25% and 77.50%, respectively. The expression of these apoptosisassociated proteins in CRC tissue was correlated with the expression of FHIT. The mean apoptosis index in FHIT negative tumors was significantly lower than that in FHIT- positive tumors （5.41 ± 0.23 vs 0.56 ± 0.10, P 〈 0.01）. CONCLUSION： The FHIT gene plays an important role in the regulation of apoptosis and decreased FHIT expression plays a key role in the initiation and progression of colorectal carcinoma.展开更多
A substantial reduction in groundwater level,exacerbated by coal mining activities,is intensifying water scarcity in western China’s ecologically fragile coal mining areas.China’s national strategic goal of achievin...A substantial reduction in groundwater level,exacerbated by coal mining activities,is intensifying water scarcity in western China’s ecologically fragile coal mining areas.China’s national strategic goal of achieving a carbon peak and carbon neutrality has made eco-friendly mining that prioritizes the protection and efficient use of water resources essential.Based on the resource characteristics of mine water and heat hazards,an intensive coal-water-thermal collaborative co-mining paradigm for the duration of the mining process is proposed.An integrated system for the production,supply,and storage of mining companion resources is achieved through technologies such as roof water inrush prevention and control,hydrothermal quality improvement,and deep-injection geological storage.An active preventive and control system achieved by adjusting the mining technology and a passive system centered on multiobjective drainage and grouting treatment are suggested,in accordance with the original geological characteristics and dynamic process of water inrush.By implementing advanced multi-objective drainage,specifically designed to address the“skylight-type”water inrush mode in the Yulin mining area of Shaanxi Province,a substantial reduction of 50%in water drillings and inflow was achieved,leading to stabilized water conditions that effectively ensure subsequent safe coal mining.An integrated-energy complementary model that incorporates the clean production concept of heat utilization is also proposed.The findings indicate a potential saving of 8419 t of standard coal by using water and air heat as an alternative heating source for the Xiaojihan coalmine,resulting in an impressive energy conservation of 50.2%and a notable 24.2%reduction in carbon emissions.The ultra-deep sustained water injection of 100 m^(3)·h^(-1)in a single well would not rupture the formation or cause water leakage,and 7.87×10^(5)t of mine water could be effectively stored in the Liujiagou Formation,presenting a viable method for mine-water management in the Ordos Basin and providing insights for green and low-carbon mining.展开更多
Identification of carriers of fragile X syndrome(FXS) with the subsequent prenatal diagnosis and knowledge of FXS-associated genetic profiles are essential for intervention in specific populations. We report the resul...Identification of carriers of fragile X syndrome(FXS) with the subsequent prenatal diagnosis and knowledge of FXS-associated genetic profiles are essential for intervention in specific populations. We report the results of carrier screening of 39,458 East Asian adult women and prenatal diagnosis from 87 FXS carriers.The prevalence of FXS carriers and full mutation fetuses was estimated to be 1/581 and 1/3124 in East Asian populations, respectively. We confirmed the validity of the current threshold of CGG trinucleotide repeats for FMR1 categorization;the integral risks of full mutation expansion were approximately 6.0%,43.8%, and 100% for premutation alleles with 55—74, 75—89, and ≥ 90 CGG repeats, respectively. The protective effect of AGG(adenine-guanine-guanine nucleotides) interruption in East Asian populations was validated, which is important in protecting premutation alleles with 75—89 CGG repeats from full mutation expansion. Finally, family history was shown not an effective indicator for FXS carrier screening in East Asian populations, and population-based screening was more cost-effective. This study provides an insight into the largest carrier screening and prenatal diagnosis for FXS in East Asian populations to date. The FXSassociated genetic profiles of East Asian populations are delineated, and population-based carrier screening is shown to be promising for FXS intervention.展开更多
Fragile X syndrome （FXS） is the most common form of inherited mental retardation, characterized by moderate-to- severe mental retardation, attention deficits, and hyperactivity. This disease results from the expansi...Fragile X syndrome （FXS） is the most common form of inherited mental retardation, characterized by moderate-to- severe mental retardation, attention deficits, and hyperactivity. This disease results from the expansion of a trinucleotide repeat （CGG） within the X-linked fragile X mental retardation l （FMR1） gene, which leads to the lack of the product of the FMR1 gene--fragile X mental retardation protein. Many mental disorders such as FXS and Rett syndrome are thought to originate during early developmental period, but recent findings have suggested the involvement of the processes in the adult nervous system. Here we outline our recent studies and initial clinical trials that may provide an approach to treat FXS in the adulthood.展开更多
AIM: To establish the role of FHIT in the pathogenesis hepatocellular carcinoma (HCC). METHODS: We examined genomic alterations, as well as, mRNA and protein expression patterns from the FHIT gene, in 48 surgically re...AIM: To establish the role of FHIT in the pathogenesis hepatocellular carcinoma (HCC). METHODS: We examined genomic alterations, as well as, mRNA and protein expression patterns from the FHIT gene, in 48 surgically resected hepatocellular carcinoma (HCC) tissues. Additionally, p53 mutations were analyzed. RESULTS: Aberrant FHIT transcripts were detected in 11 of 48 surrounding non-tumor liver tissues and 27 of 48 HCC samples (22.9% vs 56.3%, P = 0.002). No point mutations were identified within the open reading frame region of FHIT. Loss of heterozygosity (LOH) of the FHIT locus was detected in 4 of 42 informative cases for D3S1300, and 3 of 29 informative cases for D3S1313. Reduced expression of FHIT protein (Fhit) was observed in 8 (16.7%) of 48 HCC samples, with complete loss of Fhit in only 1 case. There were no associations with abnormal transcripts, LOH, and Fhit expression. p53 mutations were identified in 9 of the 48 HCC cases. However, none of the cases displayed a G to T transversion at p53 codon 249. CONCLUSION: Aberrant FHIT transcripts were more common in HCC tissues as compared to non-cancerous liver tissues. However, Fhit expression was lost or reduced in a minor fraction of HCC tissues, while it was strongly expressed in non-cancerous liver tissues.Therefore, our study suggests that FHIT plays a role in relatively few HCC cases in South Korea.展开更多
AIM:To evaluate the inhibitory effects of human fragile histidine triad(FHIT) gene on cell proliferation and apoptosis in human hepatocellular carcinoma line Hep3B in vitro. METHODS:A recombinant pcDNA3.1(+) /FHIT inc...AIM:To evaluate the inhibitory effects of human fragile histidine triad(FHIT) gene on cell proliferation and apoptosis in human hepatocellular carcinoma line Hep3B in vitro. METHODS:A recombinant pcDNA3.1(+) /FHIT including the functional region of FHIT gene was constructed and transferred into human hepatocellular carcinoma cells in vitro. mRNA and protein expression of the FHIT gene in the transfected cells was detected by RT-PCR and Western blot,respectively. The effect of FHIT on proliferation was detected by MTT assay. Changes in cell cycle and apoptosis were assayed by flow cytometry. Five mice received subcutaneous transplantation of Hep3B-FHIT;5 mice received subcutaneous transplantation of normal Hep3B and Hep3B-C as controls. The body weight of nude mice and tumor growth were measured. RESULTS:RT-PCR and Western blot analysis showed that the expression level of FHIT-mRNA and FHIT protein was higher in Hep3B cells after infection withpcDNA3.1(+) /FHIT. The growth of Hep3B cells treated with pcDNA3.1(+) /FHIT was significantly inhibited. The pcDNA3.1(+) /FHIT-transfected Hep3B cells showed a significantly higher cell rate at G0-G1 phase and increased apoptosis in comparison with controls(P < 0.05) . The growth of transplanted tumor was inhibited markedly by FHIT. Tumors arising from the Hep3B-FHIT cells occurred much later than those arising from the Hep3B and Hep3B-C cells. The growth of Hep3B-FHIT cells was slow and the tumor volume was low. CONCLUSION:Transduction of FHIT gene inhibits the growth of human hepatocellular carcinoma cells and induces cell apoptosis in vivo and in vitro.展开更多
In the present study,Fmr1 knockout mice （KO mice） were used as the model for fragile X syndrome.The results of step-through and step-down tests demonstrated that Fmr1 KO mice had shorter latencies and more error cou...In the present study,Fmr1 knockout mice （KO mice） were used as the model for fragile X syndrome.The results of step-through and step-down tests demonstrated that Fmr1 KO mice had shorter latencies and more error counts,indicating a learning and memory disorder.After treatment with 30,60,90,120,or 200 mg/kg lithium chloride,the learning and memory abilities of the Fmr1 KO mice were significantly ameliorated,in particular,the 200 mg/kg lithium chloride treatment had the most significant effect.Western blot analysis showed that lithium chloride significantly enhanced the expression of phosphorylated glycogen synthase kinase 3 beta,an inactive form of glycogen synthase kinase 3 beta,in the cerebral cortex and hippocampus of the Fmr1 KO mice.These results indicated that lithium chloride improved learning and memory in the Fmr1 KO mice,possibly by inhibiting glycogen synthase kinase 3 beta activity.展开更多
AIM:To characterize aberrant crypt focus (ACF) in adjoining mucosa in sporadic colorectal carcinoma and to evaluate fragile histidine triad (Fhit) protein and Ki67. METHODS:ACF was identified grossly and classified hi...AIM:To characterize aberrant crypt focus (ACF) in adjoining mucosa in sporadic colorectal carcinoma and to evaluate fragile histidine triad (Fhit) protein and Ki67. METHODS:ACF was identified grossly and classified histologically in 75 resected specimens. ACF was typed into hyperplastic ACF (HACF) and dysplastic ACF (DACF). Sections of ACF, carcinoma and normal colonic mucosa as control were studied for Fhit and Ki67 expressions by immunohistochemistry and were grouped according to staining intensity and the number of positive stained cells observed in different histological groups. Comparison was done between the different groups by Pearson's χ 2 test and γ test for the ordinal data. P value < 0.05 was considered as significant.RESULTS:Age range was 40 to 86 years in males (mean = 43.36) and 45 to 70 years in females (mean = 56). HACF was identified in all cases studied in the non-tumorous colonic mucosa; ACF was observed as non-contiguous scattered foci, which supports the hypothesis of acquisition of single focus monoclonality by colonic epithelial cells in tumor generation. Twenty-four (32%) had DACF and were observed as closure to carcinoma foci. Intensity of Fhit expression:(1) HACF 40% exhibited strong intensity, similar to normal, moderate in 36% and weak in 24%; (2) DACF strong in 25%, moderate in 37.5% and weak in 37.5%; and (3) carcinoma negative in 16%, strong in 43% and moderate and weak in 28.5% each. Significant difference was observed in intensity of the Fhit protein expressions by HACF and DACF (P < 0.05). Tumor in older patients showed a stronger Fhit intensity compared to younger patients (P = 0.036). Vegetarian diet intake and nonsmokers showed stronger Fhit intensities. Advanced stage tumor, non-vegetarian diet and younger age was associated with loss of Fhit protein. Ki67 positivity was an extended crypt pattern in HACF and DACF showed extension up to the neck region of the crypts and surface epithelium. Carcinomas showed a marked increase in Ki67 expression (P < 0.05). Fhit protein had an inverse association with Ki67 expression. CONCLUSION:Weaker Fhit intensity was associated with smoking, non-vegetarian diet intake and increasing Ki67 expression. Loss of Fhit protein expression is possibly influenced by environmental factors like smoking and non-vegetarian diet intake.展开更多
Coal is the most important energy in China, accounting for about 60% of energy consumption, which is about half of the total coal output in the world. With the excavation of coal resources, a lot of land was damaged a...Coal is the most important energy in China, accounting for about 60% of energy consumption, which is about half of the total coal output in the world. With the excavation of coal resources, a lot of land was damaged and serious environmental problems were produced.展开更多
On the basis of the soil environment investigation in Da＇an City, Jilin Province, China, 40 soil samples from main land use types were obtained and tested by standard method. Soil organic matter （SOM）, total N （TN...On the basis of the soil environment investigation in Da＇an City, Jilin Province, China, 40 soil samples from main land use types were obtained and tested by standard method. Soil organic matter （SOM）, total N （TN）, total P （TP）, total K （TK）, available N （AN）, available P （AP） and available K （AK） were chosen as the evaluation factors. A regional soil nutrient evaluation model was developed based on the matter-element model. The results show that the soil samples with nutrient grade Ⅱ-Ⅴ respectively account for 10%, 30%, 32.5% and 27.5%, and those with grade Ⅳ and Ⅴ account for 60% in all samples. The relationship between soil nutrients and land types indicates that the nutrients of farmland are relatively good, with 41.7% of soil samples with the nutrient grade Ⅳ and Ⅴ. The nutrients of saline-alkali land and sandy land are the worst, with 100% of soil samples with the nutrient grade IV and V. And the ratios of soil samples grade IV and V in grassland and wasteland are respectively 62.5 % and 54.55%. Generally speaking, the soil nutrients status in Da＇an City is poor, 60% of soil samples are in poor and extremely poor conditions, indicating that the soil has been severely eroded. Being a relatively superior evaluation method with more accurate resuits and spatial distribution consistency, matter-element analysis is more suitable for regional soil nutrient evaluation than previous models.展开更多
AIM： To investigate the expression of fragile histidine triad （FHIT） protein in 64 patients with ulcerative colitis （UC） and Crohn＇s disease （CD）, and its relation with clinicopathological data. METHODS： Rabb...AIM： To investigate the expression of fragile histidine triad （FHIT） protein in 64 patients with ulcerative colitis （UC） and Crohn＇s disease （CD）, and its relation with clinicopathological data. METHODS： Rabbit-anti-FHIT antibody was used to detect FHIT protein expression in 64 formalin-fixed, paraffin-embedded tissue specimens of inflammatory bowel disease （IBD） by citrate-microwave-streptavidin （SP）-HRP immunohistochemical method. RESULTS： The positive FHIT protein expression was 22.79% ± 16.16%, 42.14% ± 16.82% in active and remittent phases of UC, 36.07% ± 19.23% in CD, and 57.05% ±8.86% in normal colon mucosa. Statistically significant differences in FHIT protein expression were observed between the active and remittent phases of UC, between the active phase of UC and normal colon mucosa, as well as between the remittent phase of UC and normal colon mucosa, and between CD and normal colon mucosa. CONCLUSION： Our results show that FHIT protein expression is completely absent or reduced in IBD, suggesting that the FHIT gene might be associated with the oncogenesis and progression of IBD, an early event from inflammatory conditions to carcinoma in IBD.展开更多
基金Open access funding provided by Ben-Gurion University.
文摘The SafeAmpCase is an innovative 3D-printed solution developed to address critical challenges in transporting and storing fragile glass drug ampoules during emergencies.This study employs a multidisciplinary approach—integrating biomedical engineering,advanced materials science,and emergency medicine expertise—to develop a compact,durable,and user-friendly ampoule case.A key innovation lies in the strategic selection of thermoplastic polyurethane(TPU)as the material,leveraging its superior impact resistance,flexibility,and noise-damping characteristics to ensure reliability under performance in demanding real-world conditions.To optimize the 3D printing process,key parameters,including printing temperature(220-250℃),volumetric flow rate(3-20 mm^(3)/s),retraction speed(30-90 mm/s),and retraction length(0.4-1.2 mm),were systematically adjusted using calibration models.The final optimized parameters(245℃,7 mm^(3)/s,90 mm/s,and 1.2 mm)reduced production time by 43%while preserving structural integrity.American Society for Testing and Materials(ASTM)international standard drop tests confirmed the case’s exceptional impact resistance,demonstrating a 90%reduction in ampoule breakage compared to polylactic acid plus.Further refinements,guided by feedback from 25 emergency professionals,resulted in medicationspecific color coding and an enhanced locking mechanism for usability in high-pressure situations.The final SafeAmpCase model withstood 18 consecutive drop trials without ampoule breakage,confirming its robustness in field conditions.This research underscores the transformative potential of additive manufacturing in developing customized,high-performance solutions for critical healthcare applications,setting a new benchmark for biomedical device design and rapid prototyping.
基金The National Natural Science Foundation of China under contract Nos 32270219 and 31970216.
文摘Biological invasions have become recognized as one of the greatest threats to ecosystems.Codium,a genus of invasive green algae,has frequent global outbreaks and damages local marine ecosystems.It is now generally accepted that light is one of the main factors affecting the luxuriant growth of macroalgae such as Codium.In this study,to investigate the invasive photo-adaptation strategy of Codium fragile,the photo-adaptation characteristics of C.fragile and Codium.cylindricum from the Nan’ao Island of China were compared and explored.The effect of light intensity on the photosynthetic properties of the two species was investigated:the maximum quantum yield of photosystemⅡ(Fv/Fm)of C.fragile was significantly higher at low light intensity.At a light intensity of 90μmol/(m^(2)·s),maximum relative electron transport rate(rETR_(max))of the thalli was maximum,and the minimum saturating irradiance(Ek)was significantly increased.The photosynthetic rate(αvalue)of thalli was highest at a light intensity of 30μmol/(m^(2)·s).The photochemical quenching(qP)was enhanced but non-photochemical quenching(NPQ)was reduced at high light intensities.As for C.cylindricum,the optimal photochemical efficiency of the thalli at low light intensity was higher.High light intensity significantly reduced the rETR of the thalli.At low light intensity,αwas significantly higher,Ek was significantly lower,and NPQ was also significantly decreased.The response relationship between light acclimation and antioxidant capacity of the thalli of two species of Codium was investigated:there was no significant effect of light intensity variation on the total antioxidant capacity of C.fragile.In the case of C.cylindricum,the degree of membrane lipid peroxidation was significantly increased at low light intensity,and its antioxidant capacity was significantly reduced when the light intensity was too high or too low.It can be hypothesized that the self-protection ability of C.fragile may be stronger than that of C.cylindricum under low and high light intensities,which is closely related to the strong invasiveness of C.fragile.
文摘Biometric template protection is essential for finger-based authentication systems,as template tampering and adversarial attacks threaten the security.This paper proposes a DCT-based fragile watermarking scheme incorporating AI-based tamper detection to improve the integrity and robustness of finger authentication.The system was tested against NIST SD4 and Anguli fingerprint datasets,wherein 10,000 watermarked fingerprints were employed for training.The designed approach recorded a tamper detection rate of 98.3%,performing 3–6%better than current DCT,SVD,and DWT-based watermarking approaches.The false positive rate(≤1.2%)and false negative rate(≤1.5%)were much lower compared to previous research,which maintained high reliability for template change detection.The system showed real-time performance,averaging 12–18 ms processing time per template,and is thus suitable for real-world biometric authentication scenarios.Quality analysis of fingerprints indicated that NFIQ scores were enhanced from 2.07 to 1.81,reflecting improved minutiae clarity and ridge structure preservation.The approach also exhibited strong resistance to compression and noise distortions,with the improvements in PSNR being 2 dB(JPEG compression Q=80)and the SSIM values rising by 3%–5%under noise attacks.Comparative assessment demonstrated that training with NIST SD4 data greatly improved the ridge continuity and quality of fingerprints,resulting in better match scores(260–295)when tested against Bozorth3.Smaller batch sizes(batch=2)also resulted in improved ridge clarity,whereas larger batch sizes(batch=8)resulted in distortions.The DCNN-based tamper detection model supported real-time classification,which greatly minimized template exposure to adversarial attacks and synthetic fingerprint forgeries.Results demonstrate that fragile watermarking with AI indeed greatly enhances fingerprint security,providing privacy-preserving biometric authentication with high robustness,accuracy,and computational efficiency.
基金Supported by Central Public-interest Scientific Institution Basal Research Fund of China(BRF090202)~~
文摘Guazhou County of Gansu Province is a typical ecological fragile area lying in the transition area of desert and oasis.The development of its agricultural economy is highly dependent on natural resources and environment.Contradiction of resources characterized agriculture and the fragile ecological environment has become one of the main barriers to log the impact of local agriculture to achieve sustainable development.Aiming at fragile ecological environment and limiting factors of agricultural sustainable development,thoughts and countermeasures of realizing agricultural sustainable development have been proposed.
文摘[Objective] The aim was to explore eco-economy in ecological fragile region with Wuqi County in Shaanxi Province as an example. [Method] Eco-economy development and major environmental problems in Wuqi County in Shaanxi Province were researched based on local social and economic conditions, population of impoverished people and environment vulnerability. [Result] The areas with vulnerable environment and backward economy should establish a virtuous circle between economy and environment and a sustainable model of economic development. [Conclusion] It is significant to change the severe situation of environment deterioration and to promote ecological civilization.
文摘Peptide composition and arrangement of 4 major light-harvesting complexes LHCP 1-3 and LHCP 3′ isolated from siphonous green algae (Codium fragile (Sur.) Hariot.) were investigated. LHCP 1 showed five main peptides, 34.4, 31.5, 29.5, 28.2 and 26.5 kD in SDS-PAGE, the 34.4 and 31.5 kD peptides were never found in higher plants. LHCP 3 contained the other four kinds of LHCP 1 peptides except 34.4 kD, while LHCP 3′ consisted of only 28.2 and 26.5 kD peptides. We found that 34.4, 28.2 and 26.5 kD peptides were easy to decompose from LHCP 1 when subjected to SDS-PAGE without pretreatment. They might be located at the exterior of LHCP 1, while the 31.5 and 29.5 kD peptides were at the central part. The 28.2 and 26.5 kD peptides often occurred in CPa, the center complex of PSⅡ. They are possibly the LHCⅡ peptides tightly associated with CCⅡ. According to the results described above, a peptide map of LHCP 1 was sketched.
文摘AIM: To evaluate the expression of fragile histidine triad (FHIT) gene protein, product of a candidate tumor suppressor, and to investigate the relationship between FHIT, cell apoptosis and proliferation, and pathological features of primary hepatocellular carcinoma (HCC). METHODS: Forty-seven HCC and ten normal liver specimens were collected during surgical operation between 2001 and 2003. FHIT and proliferating cell nuclear antigen (PCNA) expression were detected by immunohistochemistry, and apoptotic level was evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay on the tissue sections. RESULTS: All normal liver tissues showed a strong expression of FHIT, whereas 28 of 47 (59.6%) carcinomas showed a significant loss or absence of FHIT expression (P= 0.001). The proportion of reduced FHIT expression in those carcinomas at stages Ⅲ-Ⅳ (70.6%) and in those with extrahepatic metastasis (86.7%) showed an increasing trend compared with those at stages HI (30.8%, P= 0.013) and those without metastasis (46.9%, P = 0.010) respectively. Apoptotic incidence in advanced TNM stage carcinoma and those with positive FHIT expression was higher than that in early stage carcinoma (P=0.030) and in those with negative FHIT expression (P=0.044) respectively. The proliferating potential of hepatocellular carcinoma was associated with FHIT expression (P= 0.016) and the aggressive feature (P = 0.019). Kaplan-Meier analysis demonstrated that the survival time of these 47 patients correlated with TNM stage, FHIT expression and metastasis. CONCLUSION: There is marked loss or absence of FHIT expression, as well as abnormal apoptosis-prdiferation balance in HCC. FHIT may play an important role in carcinogenesis and development of HCC.
文摘Fragile X syndrome （FXS） is one of the most prevalent mental retardations. It is mainly caused by the loss of fragile X mental retardation protein （FMRP）. FMRP is an RNA binding protein and can regulate the translation of its binding RNA, thus regulate several signaling pathways. Many FXS patients show high susceptibility to epilepsy. Epilepsy is a chronic neurological disorder which is characterized by the recurrent appearance of spontaneous seizures due to neuronal hyperactivity in the brain. Both the abnormal activation of several signaling pathway and morphological abnormality that are caused by the loss of FMRP can lead to a high susceptibility to epilepsy. Combining with the research progresses on both FXS and epilepsy, we outlined the possible mechanisms of high susceptibility to epilepsy in FXS and tried to give a prospect on the future research on the mechanism of epilepsy that happened in other mental retardations.
基金Supported by the Natural Science Foundation of Guangdong Province, No. 06020005
文摘AIM： To detect the expression of fragile histidine triad （FHIT） in normal colorectal tissue, colorectal adenoma and colorectal cancer （CRC） tissue, and to analyze its relationship with the clinicopathological features of CRC, and apoptosis-associatecl proteins （Bcl-2, Bax, survivin） and apoptosis in colorectal cancer. METHODS： FHIT mRNA analysis was performed by nested reverse transcription-polymerase chain reaction （RT-PCR） assay. Tissue microarray （TMA） was established to detect the expression of FHIT, Bcl-2, Bax and survivin genes in 80 CRC tissue specimens, 16 colorectal adenoma tissue specimens and 16 hemorrhoid （PPH） tissue specimens during the same period of time as the control. Citrate-microwave-SP was used as immunohistochemical method. The relationship between clinicopathological factors, such as differentiation grades and 5-year survival rate was observed. TUNEL assay was used to detect the apoptosis index in 80 CRC tissue specimens. RESULTS： Ten out of 26 （38.5%） CRC tissue specimens expressed aberrant FHIT transcripts, none of the aberrant FHIT transcripts was observed in the matchednormal tissue and colorectal adenoma tissue by nested RT-PCR assay. The positive rate of FHIT gene expression in normal colorectal tissue, colorectal adenoma and carcinoma tissue was 93.75%, 68.75% and 46.25%, respectively. Clinicopathological analysis of patients showed that the decreased FHIT gene expression was not associated with age, sex, serum CEA levels, tumor site and size, histological classification. However, the expression of FHIT was correlated with differentiation grades, pathological stages, lymph node metastases and 5-year survival rate after operation. The positive rate of apoptosis-associated proteins （Bax, Bcl-2 and survivin） in CRC tissue was 72.50%, 51.25% and 77.50%, respectively. The expression of these apoptosisassociated proteins in CRC tissue was correlated with the expression of FHIT. The mean apoptosis index in FHIT negative tumors was significantly lower than that in FHIT- positive tumors （5.41 ± 0.23 vs 0.56 ± 0.10, P 〈 0.01）. CONCLUSION： The FHIT gene plays an important role in the regulation of apoptosis and decreased FHIT expression plays a key role in the initiation and progression of colorectal carcinoma.
基金supported by the National Key Research and Development Program of China(2021YFC2902004)the National Natural Science Foundation of China(42072284,42027801,and 41877186).
文摘A substantial reduction in groundwater level,exacerbated by coal mining activities,is intensifying water scarcity in western China’s ecologically fragile coal mining areas.China’s national strategic goal of achieving a carbon peak and carbon neutrality has made eco-friendly mining that prioritizes the protection and efficient use of water resources essential.Based on the resource characteristics of mine water and heat hazards,an intensive coal-water-thermal collaborative co-mining paradigm for the duration of the mining process is proposed.An integrated system for the production,supply,and storage of mining companion resources is achieved through technologies such as roof water inrush prevention and control,hydrothermal quality improvement,and deep-injection geological storage.An active preventive and control system achieved by adjusting the mining technology and a passive system centered on multiobjective drainage and grouting treatment are suggested,in accordance with the original geological characteristics and dynamic process of water inrush.By implementing advanced multi-objective drainage,specifically designed to address the“skylight-type”water inrush mode in the Yulin mining area of Shaanxi Province,a substantial reduction of 50%in water drillings and inflow was achieved,leading to stabilized water conditions that effectively ensure subsequent safe coal mining.An integrated-energy complementary model that incorporates the clean production concept of heat utilization is also proposed.The findings indicate a potential saving of 8419 t of standard coal by using water and air heat as an alternative heating source for the Xiaojihan coalmine,resulting in an impressive energy conservation of 50.2%and a notable 24.2%reduction in carbon emissions.The ultra-deep sustained water injection of 100 m^(3)·h^(-1)in a single well would not rupture the formation or cause water leakage,and 7.87×10^(5)t of mine water could be effectively stored in the Liujiagou Formation,presenting a viable method for mine-water management in the Ordos Basin and providing insights for green and low-carbon mining.
基金supported by the National Natural Science Foundation of China(82071662,to Q.G.)。
文摘Identification of carriers of fragile X syndrome(FXS) with the subsequent prenatal diagnosis and knowledge of FXS-associated genetic profiles are essential for intervention in specific populations. We report the results of carrier screening of 39,458 East Asian adult women and prenatal diagnosis from 87 FXS carriers.The prevalence of FXS carriers and full mutation fetuses was estimated to be 1/581 and 1/3124 in East Asian populations, respectively. We confirmed the validity of the current threshold of CGG trinucleotide repeats for FMR1 categorization;the integral risks of full mutation expansion were approximately 6.0%,43.8%, and 100% for premutation alleles with 55—74, 75—89, and ≥ 90 CGG repeats, respectively. The protective effect of AGG(adenine-guanine-guanine nucleotides) interruption in East Asian populations was validated, which is important in protecting premutation alleles with 75—89 CGG repeats from full mutation expansion. Finally, family history was shown not an effective indicator for FXS carrier screening in East Asian populations, and population-based screening was more cost-effective. This study provides an insight into the largest carrier screening and prenatal diagnosis for FXS in East Asian populations to date. The FXSassociated genetic profiles of East Asian populations are delineated, and population-based carrier screening is shown to be promising for FXS intervention.
基金supported by the National Natural Science Foundation of China (No. 30670684,30770686)
文摘Fragile X syndrome （FXS） is the most common form of inherited mental retardation, characterized by moderate-to- severe mental retardation, attention deficits, and hyperactivity. This disease results from the expansion of a trinucleotide repeat （CGG） within the X-linked fragile X mental retardation l （FMR1） gene, which leads to the lack of the product of the FMR1 gene--fragile X mental retardation protein. Many mental disorders such as FXS and Rett syndrome are thought to originate during early developmental period, but recent findings have suggested the involvement of the processes in the adult nervous system. Here we outline our recent studies and initial clinical trials that may provide an approach to treat FXS in the adulthood.
基金The Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund), KRF-2007-412-J00303Ulsan University Hospital Biomedical Research Center Promotion Fund (UUH-2007-05)
文摘AIM: To establish the role of FHIT in the pathogenesis hepatocellular carcinoma (HCC). METHODS: We examined genomic alterations, as well as, mRNA and protein expression patterns from the FHIT gene, in 48 surgically resected hepatocellular carcinoma (HCC) tissues. Additionally, p53 mutations were analyzed. RESULTS: Aberrant FHIT transcripts were detected in 11 of 48 surrounding non-tumor liver tissues and 27 of 48 HCC samples (22.9% vs 56.3%, P = 0.002). No point mutations were identified within the open reading frame region of FHIT. Loss of heterozygosity (LOH) of the FHIT locus was detected in 4 of 42 informative cases for D3S1300, and 3 of 29 informative cases for D3S1313. Reduced expression of FHIT protein (Fhit) was observed in 8 (16.7%) of 48 HCC samples, with complete loss of Fhit in only 1 case. There were no associations with abnormal transcripts, LOH, and Fhit expression. p53 mutations were identified in 9 of the 48 HCC cases. However, none of the cases displayed a G to T transversion at p53 codon 249. CONCLUSION: Aberrant FHIT transcripts were more common in HCC tissues as compared to non-cancerous liver tissues. However, Fhit expression was lost or reduced in a minor fraction of HCC tissues, while it was strongly expressed in non-cancerous liver tissues.Therefore, our study suggests that FHIT plays a role in relatively few HCC cases in South Korea.
文摘AIM:To evaluate the inhibitory effects of human fragile histidine triad(FHIT) gene on cell proliferation and apoptosis in human hepatocellular carcinoma line Hep3B in vitro. METHODS:A recombinant pcDNA3.1(+) /FHIT including the functional region of FHIT gene was constructed and transferred into human hepatocellular carcinoma cells in vitro. mRNA and protein expression of the FHIT gene in the transfected cells was detected by RT-PCR and Western blot,respectively. The effect of FHIT on proliferation was detected by MTT assay. Changes in cell cycle and apoptosis were assayed by flow cytometry. Five mice received subcutaneous transplantation of Hep3B-FHIT;5 mice received subcutaneous transplantation of normal Hep3B and Hep3B-C as controls. The body weight of nude mice and tumor growth were measured. RESULTS:RT-PCR and Western blot analysis showed that the expression level of FHIT-mRNA and FHIT protein was higher in Hep3B cells after infection withpcDNA3.1(+) /FHIT. The growth of Hep3B cells treated with pcDNA3.1(+) /FHIT was significantly inhibited. The pcDNA3.1(+) /FHIT-transfected Hep3B cells showed a significantly higher cell rate at G0-G1 phase and increased apoptosis in comparison with controls(P < 0.05) . The growth of transplanted tumor was inhibited markedly by FHIT. Tumors arising from the Hep3B-FHIT cells occurred much later than those arising from the Hep3B and Hep3B-C cells. The growth of Hep3B-FHIT cells was slow and the tumor volume was low. CONCLUSION:Transduction of FHIT gene inhibits the growth of human hepatocellular carcinoma cells and induces cell apoptosis in vivo and in vitro.
基金the National Natural Science Foundation of China,No.30870876the Natural Science Foundation of Guangdong Province,No.815101700100005+2 种基金the Science and Technology Program of Guangdong Province,No.2005B60302004,2008B030301371,2009B030801368the Traditional Chinese Medicineand Combination of Traditional Chinese and Western Medicine Program of Guangzhou,No.2008A52the Medical and Health Scientific Research Program of Guangzhou,No.2009-YB-167
文摘In the present study,Fmr1 knockout mice （KO mice） were used as the model for fragile X syndrome.The results of step-through and step-down tests demonstrated that Fmr1 KO mice had shorter latencies and more error counts,indicating a learning and memory disorder.After treatment with 30,60,90,120,or 200 mg/kg lithium chloride,the learning and memory abilities of the Fmr1 KO mice were significantly ameliorated,in particular,the 200 mg/kg lithium chloride treatment had the most significant effect.Western blot analysis showed that lithium chloride significantly enhanced the expression of phosphorylated glycogen synthase kinase 3 beta,an inactive form of glycogen synthase kinase 3 beta,in the cerebral cortex and hippocampus of the Fmr1 KO mice.These results indicated that lithium chloride improved learning and memory in the Fmr1 KO mice,possibly by inhibiting glycogen synthase kinase 3 beta activity.
文摘AIM:To characterize aberrant crypt focus (ACF) in adjoining mucosa in sporadic colorectal carcinoma and to evaluate fragile histidine triad (Fhit) protein and Ki67. METHODS:ACF was identified grossly and classified histologically in 75 resected specimens. ACF was typed into hyperplastic ACF (HACF) and dysplastic ACF (DACF). Sections of ACF, carcinoma and normal colonic mucosa as control were studied for Fhit and Ki67 expressions by immunohistochemistry and were grouped according to staining intensity and the number of positive stained cells observed in different histological groups. Comparison was done between the different groups by Pearson's χ 2 test and γ test for the ordinal data. P value < 0.05 was considered as significant.RESULTS:Age range was 40 to 86 years in males (mean = 43.36) and 45 to 70 years in females (mean = 56). HACF was identified in all cases studied in the non-tumorous colonic mucosa; ACF was observed as non-contiguous scattered foci, which supports the hypothesis of acquisition of single focus monoclonality by colonic epithelial cells in tumor generation. Twenty-four (32%) had DACF and were observed as closure to carcinoma foci. Intensity of Fhit expression:(1) HACF 40% exhibited strong intensity, similar to normal, moderate in 36% and weak in 24%; (2) DACF strong in 25%, moderate in 37.5% and weak in 37.5%; and (3) carcinoma negative in 16%, strong in 43% and moderate and weak in 28.5% each. Significant difference was observed in intensity of the Fhit protein expressions by HACF and DACF (P < 0.05). Tumor in older patients showed a stronger Fhit intensity compared to younger patients (P = 0.036). Vegetarian diet intake and nonsmokers showed stronger Fhit intensities. Advanced stage tumor, non-vegetarian diet and younger age was associated with loss of Fhit protein. Ki67 positivity was an extended crypt pattern in HACF and DACF showed extension up to the neck region of the crypts and surface epithelium. Carcinomas showed a marked increase in Ki67 expression (P < 0.05). Fhit protein had an inverse association with Ki67 expression. CONCLUSION:Weaker Fhit intensity was associated with smoking, non-vegetarian diet intake and increasing Ki67 expression. Loss of Fhit protein expression is possibly influenced by environmental factors like smoking and non-vegetarian diet intake.
文摘Coal is the most important energy in China, accounting for about 60% of energy consumption, which is about half of the total coal output in the world. With the excavation of coal resources, a lot of land was damaged and serious environmental problems were produced.
基金Under the auspices of National Natural Science Foundation of China (No. 40572170, 40871088 )
文摘On the basis of the soil environment investigation in Da＇an City, Jilin Province, China, 40 soil samples from main land use types were obtained and tested by standard method. Soil organic matter （SOM）, total N （TN）, total P （TP）, total K （TK）, available N （AN）, available P （AP） and available K （AK） were chosen as the evaluation factors. A regional soil nutrient evaluation model was developed based on the matter-element model. The results show that the soil samples with nutrient grade Ⅱ-Ⅴ respectively account for 10%, 30%, 32.5% and 27.5%, and those with grade Ⅳ and Ⅴ account for 60% in all samples. The relationship between soil nutrients and land types indicates that the nutrients of farmland are relatively good, with 41.7% of soil samples with the nutrient grade Ⅳ and Ⅴ. The nutrients of saline-alkali land and sandy land are the worst, with 100% of soil samples with the nutrient grade IV and V. And the ratios of soil samples grade IV and V in grassland and wasteland are respectively 62.5 % and 54.55%. Generally speaking, the soil nutrients status in Da＇an City is poor, 60% of soil samples are in poor and extremely poor conditions, indicating that the soil has been severely eroded. Being a relatively superior evaluation method with more accurate resuits and spatial distribution consistency, matter-element analysis is more suitable for regional soil nutrient evaluation than previous models.
基金Supported by grant from Wuhan Municipal Government Science and Technology Department No. 301121017
文摘AIM： To investigate the expression of fragile histidine triad （FHIT） protein in 64 patients with ulcerative colitis （UC） and Crohn＇s disease （CD）, and its relation with clinicopathological data. METHODS： Rabbit-anti-FHIT antibody was used to detect FHIT protein expression in 64 formalin-fixed, paraffin-embedded tissue specimens of inflammatory bowel disease （IBD） by citrate-microwave-streptavidin （SP）-HRP immunohistochemical method. RESULTS： The positive FHIT protein expression was 22.79% ± 16.16%, 42.14% ± 16.82% in active and remittent phases of UC, 36.07% ± 19.23% in CD, and 57.05% ±8.86% in normal colon mucosa. Statistically significant differences in FHIT protein expression were observed between the active and remittent phases of UC, between the active phase of UC and normal colon mucosa, as well as between the remittent phase of UC and normal colon mucosa, and between CD and normal colon mucosa. CONCLUSION： Our results show that FHIT protein expression is completely absent or reduced in IBD, suggesting that the FHIT gene might be associated with the oncogenesis and progression of IBD, an early event from inflammatory conditions to carcinoma in IBD.
