C_(2)H_(2)zinc finger transcription factors such as FlbC and Msn2,have broad regulatory roles in fungal growth and conidiation.In the present study,we cloned and characterized a C_(2)H_(2)zinc finger transcription fac...C_(2)H_(2)zinc finger transcription factors such as FlbC and Msn2,have broad regulatory roles in fungal growth and conidiation.In the present study,we cloned and characterized a C_(2)H_(2)zinc finger transcription factor gene,FpCzf14,in the wheat pathogen Fusarium pseudograminearum.FpCzf14 was localized to the nuclei.The expression of FpCzf14 was significantly upregulated in conidia,suggesting that FpCzf14 might contribute to conidiation.Further analysis of the fpczf14-deleted mutant(Δfpczf14)demonstrated that it exhibited defect in conidiation,and this defect was restored in the complemented strainΔfpczf14-C expressing FpCzf14,demonstrating that FpCzf14 was essential for conidiation.Moreover,FpCzf14 was required for mycelial growth and pathogenicity of F.pseudograminearum.Microscopic observation results showed thatΔfpczf14 produced only very few penetration pegs and invasive hyphae inside host tissues compared with WT andΔfpczf14-C.Additionally,results of reverse transcription quantitative PCR(RT-qPCR)showed that FpCzf14 regulated expression of several conidiation-related genes in F.pseudograminearum.In conclusion,FpCzf14,as a core regulatory gene in conidiation,provides new insights into the mechanism of conidiation in F.pseudograminearum.展开更多
基金supported by grants from the International(Regional)Cooperation and Exchange Program of National Natural Science Foundation of China(31961143018)the National Key R&D Plan of China(2017YFD0301104).
文摘C_(2)H_(2)zinc finger transcription factors such as FlbC and Msn2,have broad regulatory roles in fungal growth and conidiation.In the present study,we cloned and characterized a C_(2)H_(2)zinc finger transcription factor gene,FpCzf14,in the wheat pathogen Fusarium pseudograminearum.FpCzf14 was localized to the nuclei.The expression of FpCzf14 was significantly upregulated in conidia,suggesting that FpCzf14 might contribute to conidiation.Further analysis of the fpczf14-deleted mutant(Δfpczf14)demonstrated that it exhibited defect in conidiation,and this defect was restored in the complemented strainΔfpczf14-C expressing FpCzf14,demonstrating that FpCzf14 was essential for conidiation.Moreover,FpCzf14 was required for mycelial growth and pathogenicity of F.pseudograminearum.Microscopic observation results showed thatΔfpczf14 produced only very few penetration pegs and invasive hyphae inside host tissues compared with WT andΔfpczf14-C.Additionally,results of reverse transcription quantitative PCR(RT-qPCR)showed that FpCzf14 regulated expression of several conidiation-related genes in F.pseudograminearum.In conclusion,FpCzf14,as a core regulatory gene in conidiation,provides new insights into the mechanism of conidiation in F.pseudograminearum.
