Staphylococcus aureus(S.aureus)is the third most common pathogen causing 10.6%of bacterial foodborne illnesses in China in 2021[1].Heat-stable Staphylococcal Enterotoxins(SEs)produced by S.aureus are the main contribu...Staphylococcus aureus(S.aureus)is the third most common pathogen causing 10.6%of bacterial foodborne illnesses in China in 2021[1].Heat-stable Staphylococcal Enterotoxins(SEs)produced by S.aureus are the main contributors to staphylococcal food poisoning(SFP),causing vomiting,diarrhea,abdominal pain,headache,muscle cramps,and other acute gastroenteritis symptoms.More than 25 SEs and staphylococcal enterotoxin-like toxins(SE/s)have been described and which together comprise a superfamily of pyrogenic toxin superantigens(SAgs)[2].展开更多
Foodborne bacteria produce biofilms and their viable but non-culturable(VBNC)formation,can affect food quality and safety.Studies have shown that these characteristics are regulated by the bacterial quorum sensing(QS)...Foodborne bacteria produce biofilms and their viable but non-culturable(VBNC)formation,can affect food quality and safety.Studies have shown that these characteristics are regulated by the bacterial quorum sensing(QS)system.Quenching the QS system of foodborne bacteria and blocking the expression of the corresponding genes may be an effective way to improve food quality and safety.Therefore,this article reviews the QS systems for foodborne bacteria,the regulatory mechanisms of QS systems in biofilm and VBNC formation and resuscitation,the research progress on quorum sensing inhibitors(QSIs)for Gram-negative and Gram-positive bacteria,and introduces QSIs from various sources.In addition,we have also summarized the current research issues on QS regulation of biofilms and VBNC formation.The systematic study of the QS phenomenon of foodborne bacteria in practical situations,the mechanism of bacterial QS cooperation-cheating,the screening of novel and highly active QSIs,the combination of QSIs and other technologies to improve their bioavailability,and the regulatory network between biofilm and VBNC formation and resuscitation are research directions that need to be paid attention to in the future.展开更多
In order to ensure food safety,controlling foodborne pathogen contamination is of utmost importance.Growing apprehensions regarding the safety of synthetic antimicrobials,due to their adverse health effects,have promp...In order to ensure food safety,controlling foodborne pathogen contamination is of utmost importance.Growing apprehensions regarding the safety of synthetic antimicrobials,due to their adverse health effects,have prompted a search for alternative options.Plant natural products(PNPs)with antimicrobial activity are being explored as a viable alternative.Among the various antimicrobial natural products studied,plant essential oils,plant flavonoids,plant polyphenols,plant polysaccharides,and plant antimicrobial peptides have been identified as potential candidates.PNPs demonstrate a diverse array of antimicrobial mechanisms,encompassing cell wall and membrane damage,interference with genetic replication,disruption of energy metabolism,and induction of oxidative stress at the single-cell level,as well as inhibition of biofilm formation and quorum sensing at the population level.Certain PNPs have been harnessed as natural antimicrobial agents for the food preservation.The utilization of encapsulation technology proves to be an effective strategy in protecting PNPs,thereby ensuring good antimicrobial efficacy,enhanced dispersibility,and controlled release within food products.The utilization of nanoemulsions,nanoliposomes,edible packaging,electrospun nanofibers,and microcapsules formed by encapsulation has enriched the ways in which PNPs can be applied in food preservation.Although PNPs have great potential in food preservation,their widespread application in the food industry is currently constrained by factors such as production costs,safety concerns,and legal considerations.Chemical synthesis and biosynthesis pathways offer viable strategies for reducing the cost of producing PNPs,and ongoing efforts to assess safety and improve regulatory frameworks are likely to facilitate the broader adoption of PNPs in food preservation practices.This article provides an overview of the main types of PNPs with antimicrobial activity and their properties,focusing on their mechanisms of action.Additionally,it summarizes the use of PNPs in food preservation and discusses the characteristics and applications of different encapsulation technologies.Lastly,the paper briefly analyzes current limitations and proposes potential future trends for this field.展开更多
Antimicrobial resistance(AMR)has become a critical global public health challenge in the 21st century.Since the initial isolation of a blaNDM-1-carrying and carbapenem-resistant Klebsiella pneumoniae from an Indian ho...Antimicrobial resistance(AMR)has become a critical global public health challenge in the 21st century.Since the initial isolation of a blaNDM-1-carrying and carbapenem-resistant Klebsiella pneumoniae from an Indian hospital in 2009[1],the escalating prevalence of New Delhi metallo-β-lactamase(NDM)-encoding genes(blaNDM)has transformed carbapenem resistance into a worldwide phenomenon,transcending national and regional boundaries[2].Up to 90 distinct NDM variants have been reported globally according to the NCBI GenBank Pathogens database.Plasmidmediated horizontal gene transfer(HGT),which occurs both within and across bacterial species,has significantly accelerated the global dissemination of blaNDM-related genes and the associated resistance[3].Carbapenem-resistant pathogens were responsible for 200,000 deaths globally in 2019[4].Although NDM-1 has been relatively well characterized[5],the epidemiological profiles of other NDM variants require continued surveillance and indepth investigation.The novel NDM-9 variant(GenBank accession no.KC999080)was first identified in 2013 from a clinically significant isolate of Klebsiella pneumoniae ST107 strain PPH1303 with a high level of resistance to carbapenems recovered from the urine culture of a pediatric patient in Beijing,China,who had acute lymphocytic leukemia and had undergone allogeneic stem cell transplantation[6].展开更多
Addressing the uncontrolled spread and increase in antibiotic resistance in methicillin-resistant Staphylococcus aureus(MRSA)will require new control strategies,particularly to improve the safety of food.Our results r...Addressing the uncontrolled spread and increase in antibiotic resistance in methicillin-resistant Staphylococcus aureus(MRSA)will require new control strategies,particularly to improve the safety of food.Our results revealed the efficacy of theaflavin-3,3′-digallate(TFBG),which is a novel polyphenol derived from tea,in targeting the key regulatory protein multiple gene regulator A(MgrA)in S.aureus.Through fluorescence anisotropy,we showed that the half maximal inhibitory concentration(IC50)of TFBG was 26.76μg/mL.TFBG uniquely counters S.aureus by regulating its virulence factors and adhesion processes rather than by killing the bacteria directly.This compound alters the expression of key virulence factors and modulates the transcription levels of genes related to adhesion in S.aureus,ultimately reducing the bacteria’s ability to adhere to fibrinogen and its hemolytic activity.Our assays confirmed that TFBG directly interacts with the MgrA protein in MRSA,and we identified critical binding sites.Our in vivo studies highlighted the potent efficacy of TFBG.TFBG administration is an innovative approach to improve food safety by diminishing MRSA virulence and reducing its pathogenicity.展开更多
Foodborne disease is a worldwide il ness and over 1 bil ion people get diseased every year. In China, thousands of foodborne diseases occur every year. It is proved that prevention is the most crucial for control ing ...Foodborne disease is a worldwide il ness and over 1 bil ion people get diseased every year. In China, thousands of foodborne diseases occur every year. It is proved that prevention is the most crucial for control ing foodborne disease. In the research, food safety monitoring system, laws and early warning system in the US and EU were introduced to explore the successful experience and monitoring or control mode suitable for China.展开更多
Helicobacter pylori(H. pylori) is an organism that is widespread in the human population and is sometimes responsible for some of the most common chronic clinical disorders of the upper gastrointestinal tract in human...Helicobacter pylori(H. pylori) is an organism that is widespread in the human population and is sometimes responsible for some of the most common chronic clinical disorders of the upper gastrointestinal tract in humans, such as chronic-active gastritis, duodenal and gastric ulcer disease, low-grade B-cell mucosa associated lymphoid tissue lymphoma of the stomach, and gastric adenocarcinoma, which is the third leading cause of cancer death worldwide. The routes of infection have not yet been firmly established, and different routes of transmission have been suggested, although the most commonly accepted hypothesis is that infection takes place through the faecal-oral route and that contaminated water and foods might play an important role in transmission of the microorganism to humans. Furthermore, several authors have considered H. pylori to be a foodborne pathogen because of some of its microbiological and epidemiological characteristics. H. pylori has been detected in drinking water, seawater, vegetables and foods of animal origin. H. pylori survives in complex foodstuffs such as milk, vegetables and ready-to-eat foods. This review article presents an overview of the present knowledge on the microbiological aspects in terms of phenotypic characteristics and growth requirements of H. pylori, focusing on the potential role that foodstuffs and water may play in the transmission of the pathogen to humans and the methods successfully used for the detection of this microorganism in foodstuffs and water.展开更多
Traditional Fourier transform infrared(FTIR)spectroscopy has been recognized as a valuable method to characterize and classify kinds of microorganisms.In this study,combined with multivariate statistical analysis,sync...Traditional Fourier transform infrared(FTIR)spectroscopy has been recognized as a valuable method to characterize and classify kinds of microorganisms.In this study,combined with multivariate statistical analysis,synchrotron radiation-based FTIR(SR-FTIR) microspectroscopy was applied to identify and discriminate ten foodborne bacterial strains.Our results show that the whole spectra(3000-900 cm^(-1)) and three subdivided spectral regions(3000-2800,1800-1500 and 1200-900 cm^(-1),representing lipids,proteins and polysaccharides,respectively) can be used to type bacteria.Either the whole spectra or the three subdivided spectra are good for discriminating the bacteria at levels of species and subspecies,but the whole spectra should be given preference at the genus level.The findings demonstrate that SR-FTIR microspectroscopy is a powerful tool to identify and classify foodborne pathogenic bacteria at the genus,species and subspecies level.展开更多
Foods are often contaminated by multiple foodborne pathogens,which threatens human health.In this work,we developed a microfluidic biosensor for multiplex immunoassay of foodborne bacteria with agitation driven by pro...Foods are often contaminated by multiple foodborne pathogens,which threatens human health.In this work,we developed a microfluidic biosensor for multiplex immunoassay of foodborne bacteria with agitation driven by programmed audio signals.This agitation,powered by the vibration of a speaker cone during music playing,accelerated the mass transport in the incubation process to form bacterial complexes within 10 min.Immunoassay reagents of the two target bacteria(Escherichia coli O157:H7 and Salmonella typhimurium)were preloaded into the corresponding fore-vacuum storage chamber on the chip,and released to participate in the subsequent immune analysis process by piercing the chambers.All the detection processes were integrated into a single microfluidic chip and controlled by a smartphone through Bluetooth.Under selected conditions,wide linear ranges and low limits of detection(LODs<2CFU/m L)were obtained,and real food samples were successfully determined within 30 min.This biosensing method can be extended to wide-ranging applications by loading different recognizing reagents.展开更多
Cases of foodborne doping are frequently reported in sports events and can cause severe consequences for athletes.The foodborne doping can be divided into natural endogenous and artifi cially added foods according to ...Cases of foodborne doping are frequently reported in sports events and can cause severe consequences for athletes.The foodborne doping can be divided into natural endogenous and artifi cially added foods according to the sources,including anabolic agents,stimulants,diuretics,β-blockers,β2 agonists and others.In order to control foodborne doping,chromatographic technique,immunoassay,nuclear magnetic resonance,biosensor technology,pyrolytic spectroscopy,comprehensive analysis and electrochemical analysis have usually used as analytical and inspection strategies.Meanwhile,the legislation of anti-doping,the improvement of testing standard and technology,and the prevention and control of food safety,as well as the improvement of risk perception of athletes are highly necessary for achieving the effective risk control and supervision of foodborne doping,which will be benefi cial for athletes,doctors and administrators to avoid the risks of foodborne doping test and reduce foodborne doping risks for the health of athletes.展开更多
Objective: To refine the infectious doses of enteric bacterial pathogens in animal assays and vaccine clinical trials by studying the invasion kinetics of five bacterial pathogens with human intestinal cells.Methods: ...Objective: To refine the infectious doses of enteric bacterial pathogens in animal assays and vaccine clinical trials by studying the invasion kinetics of five bacterial pathogens with human intestinal cells.Methods: Utilizing in vitro cultured cell invasion assays with gentamicin-killing step,the invasive effects were analyzed in foodborne pathogens including Salmonella,Shigella, Yersinia, Escherichia coli(E. coli) O157 and opportunistic pathogens Citrobacter in human embryonic intestine 407 cells and ileocecum HCT-8 cells at multiplicities of infection(MOIs) of 0.04–4 000.00 E. coli HS served as a noninvasive control.Results: The study results showed that the bacterial invasive efficiency and the average number of internalized bacteria per host cell changed with different starting MOIs. Higher starting MOIs did not always produce more bacterial internalization. The bacterial invasion effects varied with different bacterial strains and host cell lines. E. coli O157:H7 did invade human ileocecum HCT-8 cells.Conclusions: This study shows that these bacteria possess different invasive patterns at various starting MOIs and also in different cell lines. The results could help to figure out the appropriate infectious doses of the bacteria in animal assays and in vaccine clinical trials. The bacterial invasion kinetics is also valuable in evaluating the safety and efficacy of live attenuated bacterial vaccines.展开更多
Foodborne diseases(FBDs) represent a substantial, yet largely preventable, health burden and result in considerable morbidity and mortality in both developed and developing countries[1]. The FBDs Burden Epidemiology R...Foodborne diseases(FBDs) represent a substantial, yet largely preventable, health burden and result in considerable morbidity and mortality in both developed and developing countries[1]. The FBDs Burden Epidemiology Reference Group(FERG)found that the global burden of FBDs is comparable to those of major infectious diseases, such as human immunodeficiency virus infection/acquired immune deficiency syndrome, malaria, and tuberculosis[2].展开更多
Objective:Foodborne parasitic diseases,although with a declining overall incidence rate,are still endangering local public safety.This review aims to describe the current situation and epidemiological trends of foodbo...Objective:Foodborne parasitic diseases,although with a declining overall incidence rate,are still endangering local public safety.This review aims to describe the current situation and epidemiological trends of foodborne parasitic diseases in China in order to explore possible reasons contributors to its high prevalence in some areas,and propose strategies for prevention and control accordingly.Methods:A scoping review was conducted by searching Pub Med,CNKI,Wanfang,CQVIP,Embase,and the Cochrane Library using search formula"foodborne parasitic diseases(or foodborne parasites)"AND"China".Studies on foodborne parasitic diseases in China were considered,but only articles in English or Chinese published between January 1980 and June 2020 were retrieved.Included studies were screened according to the eligibility criteria:1)diseases consistent with the WHO definition of foodborne parasitic diseases;2)the food carriers were included in the WHO food classification;3)data related to epidemiology,pathogenicity,and prevention and control;4)Foodborne parasitic diseases cases or outbreaks in China.Results:A total of 111 out of 665 records were included and summarized.The prevalence of clonorchiasis,angiostrongyliasis,echinococcosis,trichinellosis and cysticercosis was still increasing although the infection rate of soil-transmitted nematodes has substantially decreased in recent years.Diverse eating habits,close contact with animals,and urbanization were contributing factors to the increase.Conclusions:Foodborne parasitic diseases remain an important public health issue in China with the progress of economic globalization and food diversification.We should manage to raise public awareness about the prevention and control of foodborne parasitic diseases,improve health and safety inspections,as well as public health practice.展开更多
Background:Understanding the global burden of enteric infections is crucial for prioritizing control strategies for foodborne and waterborne diseases.This study aimed to assess the global burden of enteric infections ...Background:Understanding the global burden of enteric infections is crucial for prioritizing control strategies for foodborne and waterborne diseases.This study aimed to assess the global burden of enteric infections in 2021 and identify risk factors from One Health aspects.Methods:Leveraging the Global Burden of Disease(GBD)2021 database,the incidence,disability-adjusted life years(DALYs),and deaths of enteric infections and the subtypes were estimated,including diarrheal diseases,typhoid and paratyphoid fever,invasive non-typhoidal Salmonella(iNTS)infections,and other intestinal infectious diseases.The estimates were quantified by absolute number,age-standardized incidence rate(ASIR),agestandardized mortality rate(ASMR)and age-standardized DALY rate with 95%uncertainty intervals(UIs).Thirteen pathogens and three risk factors associated with diarrheal diseases were analyzed.Results:In 2021,the global age-standardized DALY rate of enteric infections was 1020.15 per 100,000 popultion(95%UI:822.70-1259.39 per 100,000 population)with an estimated annual percentage change(EAPC)of-4.11%(95%confidence interval:-4.31%to-3.90%)in 1990-2021.A larger burden was observed in regions with lower socio-demographic index(SDI)levels.Diarrheal disease was the most serious subtype with Western Sub-Saharan Africa exhibiting the highest age-standardized DALY rate(2769.81 per 100,000 population,95%UI:1976.80-3674.41 per 100,000 population).Children under 5 and adults over 65 years suffered more from diarrheal diseases with the former experiencing the highest global age-standardized DALY rate(9382.46 per 100,000 population,95%UI:6771.76-13,075.12 per 100,000 population).Rotavirus remained the leading cause of diarrheal diseases despite a cross-year decline in the observed age-standardized DALY rate.Unsafe water,sanitation,and handwashing contributed most to the disease burden.Conclusion:The reduced burden of enteric infections suggested the effectiveness of previous control strategies;however,more efforts should be made in vulnerable regions and populations through a One Health approach.展开更多
Botulism is a severe neuroparalytic disease caused by Clostridium botulinums toxins.Although the disease is uncommon,causes great concern due to its high rate of mortality;foodbome outbreaks of botulism occur worldwid...Botulism is a severe neuroparalytic disease caused by Clostridium botulinums toxins.Although the disease is uncommon,causes great concern due to its high rate of mortality;foodbome outbreaks of botulism occur worldwide and require immediate public health and acute care resources.This study had a review of outstanding outbreaks published;Journals articles related to the subject.From the outbreaks analysis we found the most involved food products were;fermented fish products in Alaska;home canning food,oil preservation and restaurant sauce in London and USA;home canned vegetables,food airtight packed with inappropriate refrigeration and aerosols in Argentina.In conclusion,the diagnosis is based only on clinical findings matching the disease and previous exposure to suspicious food.Botulism must be immediately identified as one case suggests an epidemic and should be treated as a public health emergency.Therefore the purpose of the following review is to recognize the associated risks with the consumption of potentially dangerous foods,to help work our way on prevention for every public health professional to be aware of the dangers of this potentially lethal disease.展开更多
Two-dimensional(2D)nanomaterials have captured an increasing attention in biophotonics owing to their excellent optical features.Herein,2D hafnium ditelluride(HfTe_(2)),a new member of transition metal tellurides,is e...Two-dimensional(2D)nanomaterials have captured an increasing attention in biophotonics owing to their excellent optical features.Herein,2D hafnium ditelluride(HfTe_(2)),a new member of transition metal tellurides,is exploited to support gold nanoparticles fabricating HfTe_(2)-Au nanocomposites.The nanohybrids can serve as novel 2D surface-enhanced Raman scattering(SERS)substrate for the label-free detection of analyte with high sensitivity and reproducibility.Chemical mechanism originated from HfTe_(2) nanosheets and the electromagnetic enhancement induced by the hot spots on the nano-hybrids may largely contribute to the superior SERS effect of HfTe_(2)-Au nanocomposites.Finally,HfTe_(2)-Au nanocomposites are utilized for the label-free SERS analysis of foodborne pathogenic bac-teria,which realize the rapid and ultrasensitive Raman test of Escherichia coli,Listeria mono-cytogenes,Staphylococcus aureus and Salmonella with the limit of detection of 10 CFU/mL and the maximum Raman enhancement factor up to 1.7×10^(8).Combined with principal component analysis,HfTe_(2)-Au-based SERS analysis also completes the bacterial classification without extra treatment.展开更多
Infection and intoxication are two common types of foodborne illnesses throughout the world.The aim of the present work was to isolate and characterize biosurfactant producing bacteria fromgroundnut oil cake dumping s...Infection and intoxication are two common types of foodborne illnesses throughout the world.The aim of the present work was to isolate and characterize biosurfactant producing bacteria fromgroundnut oil cake dumping sites and evaluate their biocontrol effects against foodborne pathogens.Bacteria were isolated by enrichment culture technique and preliminary screening method.Biosurfactant activity evaluation was carried out by oil displacement,drop collapse test,lipase activity,hemolytic activity,emulsification index and emulsification assay methods.16s rRNA sequence analysis was used for the isolate identification.Crude biosurfactant was extracted by acid precipitation method and characterized using FTIR(Fourier Transform Infrared Spectroscopy).Antibacterial activity was investigated using disc diffusion method.CMCand surface reduction were analyzed by DuNouy tensiometer.Top 10 strains were selected for biosurfactant activity assessment from the total 30 isolates.In addition,16s rRNA sequence identified that the potential isolate was Bacillus pseudomycoides OR 1.Then,FTIR result of the extracted biosurfactant established the extract as a lipopeptide based on the absorption peaks at 3500 to 3200 cm^-1 and 2963 to 2854.68 cm^-1,respectively.50μg/mL of lipopeptide showed the highest antibacterial activity.Critical Micelle Concentration(CMC)of the lipopeptide was 60 mg/L and it reduced the surface tension of water from 71.6 to 31.6 mN/m.Hence,this study widens the scope to employ the bacterial lipopeptide surfactant as a promising biocontrol agent against foodborne pathogens.展开更多
In this work,to enhance the antibacterial potential of black rice anthocyanins for food preservation applications,cyanidin-3-O-glucoside(C3G)was isolated and subsequently acylated with capric acid using Novozym 435 li...In this work,to enhance the antibacterial potential of black rice anthocyanins for food preservation applications,cyanidin-3-O-glucoside(C3G)was isolated and subsequently acylated with capric acid using Novozym 435 lipase as the catalyst.The resulting derivative was identified as cyanidin-3-O-(6″-capryl)-glucoside(ACD).This enzy-matic modification successfully achieved its goal,as ACD exhibited significantly enhanced antibacterial activity against major foodborne pathogens.Its minimum inhibitory concentrations to Listeria monocytegenes,Staphylo-coccus aureus,and Vibrio parahaemolyticus were 50.00%-87.30%lower than those of the precursor C3G.After ACD treatment,the bacterial cells displayed twisted and sunken morphologies.The permeability of cell walls and membranes was severely compromised,as evidenced by 78.57%-346.43%increase in extracellular alkaline phosphatase activity and a 60%to 73.5%increase in nucleic acid leakage compared to untreated controls.Furthermore,ACD interfered with intracellular functions,reducing the activities of adenosine triphosphatase and superoxide dismutase by 4.58 U/mL and 4.88 U/mL,respectively,and inhibiting the tricarboxylic acid cycle by 85.2%.This study demonstrated that enzymatic acylation was an effective strategy to enhance the antibacterial efficacy of black rice anthocyanins through multi-target mechanisms,highlighting the potential of ACD as a promising candidate for development as a natural food preservative.展开更多
The aim of this study was to investigate the antibacterial,antibiofilm,antioxidant and shelf life protective po-tentials of infusion,decoction,and room temperature samples of sumac fruits prepared as used by the local...The aim of this study was to investigate the antibacterial,antibiofilm,antioxidant and shelf life protective po-tentials of infusion,decoction,and room temperature samples of sumac fruits prepared as used by the local people against three foodborne pathogens including Bacillus cereus,Escherichia coli O157:H7,and Staphylococcus aureus isolated from food samples.The room temperature samples of sumac showed a remarkable antibacterial activity(81.25%)against S.aureus.Infusion and decoction samples of sumac fruit showed an effective anti-bacterial activity(~70%)against E.coli O157:H7.Moderate efficacy against B.cereus was observed in all treatment groups.Antibiofilm effects of sumac were observed against B.cereus and S.aureus,but not against E.coli O157:H7.The total antioxidant activity was found to be close to the reference compounds.The twenty-two phenolic and flavonoid chemical compounds(gallic acid,gentisic acid,protocatechuic acid,proto-catechualdehyde,4-OH-benzoic acid,salicylic acid,chlorogenic acid,cafeic acid,p-coumaric acid,ferulic acid,rosmarinic acid,sinapic acid,trans-cinnamic acid,rutin,resveratrol,myricetin,naringenin,quercetin,luteolin,kaempferol,apigenin,and chrysin)were identified by Liquid Chromatography Mass Spectrometer(LC-MS/MS)and these molecules were analyzed by computational analyses using SwissADME,admetSAR 3.0 and the AntiBac-Pred web tool.In addition,the antibacterial efficacy of sumac fruit extracts was tested on mixed Mediterranean salad samples to evaluate the shelf-life enhancing effect.The efficacy of sumac fruit against common foodborne pathogens suggests that the ethnobotanical use of sumac may provide protection against bacterial diseases and can be utilized as an additive and/or ingredient in the food industry.展开更多
Ensuring food safety remains a global challenge because of the presence of foodborne pathogens,such as Escherichia coli and Staphylococcus aureus.In this study,we present a turn-on fluorescence biosensor based on an i...Ensuring food safety remains a global challenge because of the presence of foodborne pathogens,such as Escherichia coli and Staphylococcus aureus.In this study,we present a turn-on fluorescence biosensor based on an iron metal-organic framework(Fe-MOF)for the selective and rapid detection of two major foodborne pathogens,E.coli and S.aureus.Fe-MOF was synthesised via a solvothermal method and thoroughly characterised by Fourier-transform infrared spectroscopy(FTIR),X-ray diffraction(XRD),scanning electron microscopy with energy-dispersive X-ray spectroscopy(SEM-EDX),and thermogravimetric analysis(TGA).Upon interaction with bacterial suspensions,the sensor exhibited significant fluorescence enhancement,which was attributed to electron transfer modulation triggered by bacterial surface components.The analytical performance was evaluated across three matrices—phosphate-buffered saline(PBS),drinking water,and milk—achieving detection limits as low as 0.464 log CFU/mL for S.aureus and 0.584 log CFU/mL for E.coli.The sensor enabled rapid detection within less than 1 h with high recovery rates(95-104%)across all tested matrices,confirming its practical usability for real sample analysis.These findings address a critical gap in biosensor research by validating Fe-MOFs for practical food-safety applications in multiple real-world environments.展开更多
基金supported by the Ministry of Science and Technology of the People’s Republic of China(2022YFD1800400).
文摘Staphylococcus aureus(S.aureus)is the third most common pathogen causing 10.6%of bacterial foodborne illnesses in China in 2021[1].Heat-stable Staphylococcal Enterotoxins(SEs)produced by S.aureus are the main contributors to staphylococcal food poisoning(SFP),causing vomiting,diarrhea,abdominal pain,headache,muscle cramps,and other acute gastroenteritis symptoms.More than 25 SEs and staphylococcal enterotoxin-like toxins(SE/s)have been described and which together comprise a superfamily of pyrogenic toxin superantigens(SAgs)[2].
基金financially supported by the National Natural Science Foundation of China(32202191)and(32272279)the Key R&D Project of Shandong Province(2023CXPT007 and 2024CXPT014)the Key R&D Project of Qingdao Science and Technology Plan(24-2-3-4-zyyd-jch).
文摘Foodborne bacteria produce biofilms and their viable but non-culturable(VBNC)formation,can affect food quality and safety.Studies have shown that these characteristics are regulated by the bacterial quorum sensing(QS)system.Quenching the QS system of foodborne bacteria and blocking the expression of the corresponding genes may be an effective way to improve food quality and safety.Therefore,this article reviews the QS systems for foodborne bacteria,the regulatory mechanisms of QS systems in biofilm and VBNC formation and resuscitation,the research progress on quorum sensing inhibitors(QSIs)for Gram-negative and Gram-positive bacteria,and introduces QSIs from various sources.In addition,we have also summarized the current research issues on QS regulation of biofilms and VBNC formation.The systematic study of the QS phenomenon of foodborne bacteria in practical situations,the mechanism of bacterial QS cooperation-cheating,the screening of novel and highly active QSIs,the combination of QSIs and other technologies to improve their bioavailability,and the regulatory network between biofilm and VBNC formation and resuscitation are research directions that need to be paid attention to in the future.
基金supported by the National Natural Science Foundation of China(32060520)Science and Technology Talents and Platform Program of Yunnan Province(202105AF150049)University Key Laboratory of Food Microbial Resources and Utilization in Yunnan Province(Yunjiaofa[2018]No.135).
文摘In order to ensure food safety,controlling foodborne pathogen contamination is of utmost importance.Growing apprehensions regarding the safety of synthetic antimicrobials,due to their adverse health effects,have prompted a search for alternative options.Plant natural products(PNPs)with antimicrobial activity are being explored as a viable alternative.Among the various antimicrobial natural products studied,plant essential oils,plant flavonoids,plant polyphenols,plant polysaccharides,and plant antimicrobial peptides have been identified as potential candidates.PNPs demonstrate a diverse array of antimicrobial mechanisms,encompassing cell wall and membrane damage,interference with genetic replication,disruption of energy metabolism,and induction of oxidative stress at the single-cell level,as well as inhibition of biofilm formation and quorum sensing at the population level.Certain PNPs have been harnessed as natural antimicrobial agents for the food preservation.The utilization of encapsulation technology proves to be an effective strategy in protecting PNPs,thereby ensuring good antimicrobial efficacy,enhanced dispersibility,and controlled release within food products.The utilization of nanoemulsions,nanoliposomes,edible packaging,electrospun nanofibers,and microcapsules formed by encapsulation has enriched the ways in which PNPs can be applied in food preservation.Although PNPs have great potential in food preservation,their widespread application in the food industry is currently constrained by factors such as production costs,safety concerns,and legal considerations.Chemical synthesis and biosynthesis pathways offer viable strategies for reducing the cost of producing PNPs,and ongoing efforts to assess safety and improve regulatory frameworks are likely to facilitate the broader adoption of PNPs in food preservation practices.This article provides an overview of the main types of PNPs with antimicrobial activity and their properties,focusing on their mechanisms of action.Additionally,it summarizes the use of PNPs in food preservation and discusses the characteristics and applications of different encapsulation technologies.Lastly,the paper briefly analyzes current limitations and proposes potential future trends for this field.
基金supported financially by the National Key Research and Science Program of the Ministry of Science and Technology of the People’s Republic of China(2022YFC2303900)the Beijing Natural Science Foundation(7232242).
文摘Antimicrobial resistance(AMR)has become a critical global public health challenge in the 21st century.Since the initial isolation of a blaNDM-1-carrying and carbapenem-resistant Klebsiella pneumoniae from an Indian hospital in 2009[1],the escalating prevalence of New Delhi metallo-β-lactamase(NDM)-encoding genes(blaNDM)has transformed carbapenem resistance into a worldwide phenomenon,transcending national and regional boundaries[2].Up to 90 distinct NDM variants have been reported globally according to the NCBI GenBank Pathogens database.Plasmidmediated horizontal gene transfer(HGT),which occurs both within and across bacterial species,has significantly accelerated the global dissemination of blaNDM-related genes and the associated resistance[3].Carbapenem-resistant pathogens were responsible for 200,000 deaths globally in 2019[4].Although NDM-1 has been relatively well characterized[5],the epidemiological profiles of other NDM variants require continued surveillance and indepth investigation.The novel NDM-9 variant(GenBank accession no.KC999080)was first identified in 2013 from a clinically significant isolate of Klebsiella pneumoniae ST107 strain PPH1303 with a high level of resistance to carbapenems recovered from the urine culture of a pediatric patient in Beijing,China,who had acute lymphocytic leukemia and had undergone allogeneic stem cell transplantation[6].
文摘Addressing the uncontrolled spread and increase in antibiotic resistance in methicillin-resistant Staphylococcus aureus(MRSA)will require new control strategies,particularly to improve the safety of food.Our results revealed the efficacy of theaflavin-3,3′-digallate(TFBG),which is a novel polyphenol derived from tea,in targeting the key regulatory protein multiple gene regulator A(MgrA)in S.aureus.Through fluorescence anisotropy,we showed that the half maximal inhibitory concentration(IC50)of TFBG was 26.76μg/mL.TFBG uniquely counters S.aureus by regulating its virulence factors and adhesion processes rather than by killing the bacteria directly.This compound alters the expression of key virulence factors and modulates the transcription levels of genes related to adhesion in S.aureus,ultimately reducing the bacteria’s ability to adhere to fibrinogen and its hemolytic activity.Our assays confirmed that TFBG directly interacts with the MgrA protein in MRSA,and we identified critical binding sites.Our in vivo studies highlighted the potent efficacy of TFBG.TFBG administration is an innovative approach to improve food safety by diminishing MRSA virulence and reducing its pathogenicity.
文摘Foodborne disease is a worldwide il ness and over 1 bil ion people get diseased every year. In China, thousands of foodborne diseases occur every year. It is proved that prevention is the most crucial for control ing foodborne disease. In the research, food safety monitoring system, laws and early warning system in the US and EU were introduced to explore the successful experience and monitoring or control mode suitable for China.
文摘Helicobacter pylori(H. pylori) is an organism that is widespread in the human population and is sometimes responsible for some of the most common chronic clinical disorders of the upper gastrointestinal tract in humans, such as chronic-active gastritis, duodenal and gastric ulcer disease, low-grade B-cell mucosa associated lymphoid tissue lymphoma of the stomach, and gastric adenocarcinoma, which is the third leading cause of cancer death worldwide. The routes of infection have not yet been firmly established, and different routes of transmission have been suggested, although the most commonly accepted hypothesis is that infection takes place through the faecal-oral route and that contaminated water and foods might play an important role in transmission of the microorganism to humans. Furthermore, several authors have considered H. pylori to be a foodborne pathogen because of some of its microbiological and epidemiological characteristics. H. pylori has been detected in drinking water, seawater, vegetables and foods of animal origin. H. pylori survives in complex foodstuffs such as milk, vegetables and ready-to-eat foods. This review article presents an overview of the present knowledge on the microbiological aspects in terms of phenotypic characteristics and growth requirements of H. pylori, focusing on the potential role that foodstuffs and water may play in the transmission of the pathogen to humans and the methods successfully used for the detection of this microorganism in foodstuffs and water.
基金supported by Science Frontier Project of the Chinese Academy of Sciences(No.QYZDJ-SSW-SLH019)Open Research Fund Program of Shanghai Key Laboratory of Medical Biodefense
文摘Traditional Fourier transform infrared(FTIR)spectroscopy has been recognized as a valuable method to characterize and classify kinds of microorganisms.In this study,combined with multivariate statistical analysis,synchrotron radiation-based FTIR(SR-FTIR) microspectroscopy was applied to identify and discriminate ten foodborne bacterial strains.Our results show that the whole spectra(3000-900 cm^(-1)) and three subdivided spectral regions(3000-2800,1800-1500 and 1200-900 cm^(-1),representing lipids,proteins and polysaccharides,respectively) can be used to type bacteria.Either the whole spectra or the three subdivided spectra are good for discriminating the bacteria at levels of species and subspecies,but the whole spectra should be given preference at the genus level.The findings demonstrate that SR-FTIR microspectroscopy is a powerful tool to identify and classify foodborne pathogenic bacteria at the genus,species and subspecies level.
基金supported financially by“Kunlun Talents High-end Innovation and Entrepreneurship Talents”of Qinghai Province in 2022National Natural Science Foundation of China(Nos.22322401 and 82073816)Beijing Nova Program(No.20220484055)。
文摘Foods are often contaminated by multiple foodborne pathogens,which threatens human health.In this work,we developed a microfluidic biosensor for multiplex immunoassay of foodborne bacteria with agitation driven by programmed audio signals.This agitation,powered by the vibration of a speaker cone during music playing,accelerated the mass transport in the incubation process to form bacterial complexes within 10 min.Immunoassay reagents of the two target bacteria(Escherichia coli O157:H7 and Salmonella typhimurium)were preloaded into the corresponding fore-vacuum storage chamber on the chip,and released to participate in the subsequent immune analysis process by piercing the chambers.All the detection processes were integrated into a single microfluidic chip and controlled by a smartphone through Bluetooth.Under selected conditions,wide linear ranges and low limits of detection(LODs<2CFU/m L)were obtained,and real food samples were successfully determined within 30 min.This biosensing method can be extended to wide-ranging applications by loading different recognizing reagents.
基金financially supported by the Donghu Xuezi Program from Wuhan Sports University in China to Wei Chenthe Key Special Project of Disciplinary Development, Hubei Superior Discipline Groups of Physical Education and Health Promotionthe Chutian Scholar Program and Innovative Start-Up Foundation from Wuhan Sports University to Ning Chen。
文摘Cases of foodborne doping are frequently reported in sports events and can cause severe consequences for athletes.The foodborne doping can be divided into natural endogenous and artifi cially added foods according to the sources,including anabolic agents,stimulants,diuretics,β-blockers,β2 agonists and others.In order to control foodborne doping,chromatographic technique,immunoassay,nuclear magnetic resonance,biosensor technology,pyrolytic spectroscopy,comprehensive analysis and electrochemical analysis have usually used as analytical and inspection strategies.Meanwhile,the legislation of anti-doping,the improvement of testing standard and technology,and the prevention and control of food safety,as well as the improvement of risk perception of athletes are highly necessary for achieving the effective risk control and supervision of foodborne doping,which will be benefi cial for athletes,doctors and administrators to avoid the risks of foodborne doping test and reduce foodborne doping risks for the health of athletes.
文摘Objective: To refine the infectious doses of enteric bacterial pathogens in animal assays and vaccine clinical trials by studying the invasion kinetics of five bacterial pathogens with human intestinal cells.Methods: Utilizing in vitro cultured cell invasion assays with gentamicin-killing step,the invasive effects were analyzed in foodborne pathogens including Salmonella,Shigella, Yersinia, Escherichia coli(E. coli) O157 and opportunistic pathogens Citrobacter in human embryonic intestine 407 cells and ileocecum HCT-8 cells at multiplicities of infection(MOIs) of 0.04–4 000.00 E. coli HS served as a noninvasive control.Results: The study results showed that the bacterial invasive efficiency and the average number of internalized bacteria per host cell changed with different starting MOIs. Higher starting MOIs did not always produce more bacterial internalization. The bacterial invasion effects varied with different bacterial strains and host cell lines. E. coli O157:H7 did invade human ileocecum HCT-8 cells.Conclusions: This study shows that these bacteria possess different invasive patterns at various starting MOIs and also in different cell lines. The results could help to figure out the appropriate infectious doses of the bacteria in animal assays and in vaccine clinical trials. The bacterial invasion kinetics is also valuable in evaluating the safety and efficacy of live attenuated bacterial vaccines.
文摘Foodborne diseases(FBDs) represent a substantial, yet largely preventable, health burden and result in considerable morbidity and mortality in both developed and developing countries[1]. The FBDs Burden Epidemiology Reference Group(FERG)found that the global burden of FBDs is comparable to those of major infectious diseases, such as human immunodeficiency virus infection/acquired immune deficiency syndrome, malaria, and tuberculosis[2].
基金supported by the Major Science and Technology Program of Hainan Province(Grant No.ZDKJ202003)Grants from the Key Research and Development Program of Hainan Province(Grant No.ZDYF2020120)+7 种基金the National Natural Science Foundation of China(Grant Nos.81572023 and 81371836)the Guangdong Natural Science Foundation(Grant No.2019A1515011541)the Science and Technology Planning Project of Guangdong Province(Grant No.2019B030316025)the National Parasitic Resources Center of China(Grant No.NPRC-2019-194-30)the Open Foundation of Key Laboratory of Tropical Translational Medicine of the Ministry of EducationHainan Medical University(Grant No.2020TTM007)the 111 Project(Grant No.B12003)the Teaching Reform Project of Guangdong Province(Grant No.2017001)
文摘Objective:Foodborne parasitic diseases,although with a declining overall incidence rate,are still endangering local public safety.This review aims to describe the current situation and epidemiological trends of foodborne parasitic diseases in China in order to explore possible reasons contributors to its high prevalence in some areas,and propose strategies for prevention and control accordingly.Methods:A scoping review was conducted by searching Pub Med,CNKI,Wanfang,CQVIP,Embase,and the Cochrane Library using search formula"foodborne parasitic diseases(or foodborne parasites)"AND"China".Studies on foodborne parasitic diseases in China were considered,but only articles in English or Chinese published between January 1980 and June 2020 were retrieved.Included studies were screened according to the eligibility criteria:1)diseases consistent with the WHO definition of foodborne parasitic diseases;2)the food carriers were included in the WHO food classification;3)data related to epidemiology,pathogenicity,and prevention and control;4)Foodborne parasitic diseases cases or outbreaks in China.Results:A total of 111 out of 665 records were included and summarized.The prevalence of clonorchiasis,angiostrongyliasis,echinococcosis,trichinellosis and cysticercosis was still increasing although the infection rate of soil-transmitted nematodes has substantially decreased in recent years.Diverse eating habits,close contact with animals,and urbanization were contributing factors to the increase.Conclusions:Foodborne parasitic diseases remain an important public health issue in China with the progress of economic globalization and food diversification.We should manage to raise public awareness about the prevention and control of foodborne parasitic diseases,improve health and safety inspections,as well as public health practice.
基金supported by Bill&Melinda Gates Foundation[grant number OPP1152504]International Joint Laboratory on Tropical Diseases Control in Greater Mekong Subregion[grant number 21410750200]+3 种基金National Natural Science Foundation of China[grant number 82304102]Natural Science Foundation of Shanghai[grant number 23ZR1436200]Shanghai Science and Technology Development Foundation[grant number 22YF1421100]Shanghai Science and Technology Development Foundation[grant number 23YF1421200].
文摘Background:Understanding the global burden of enteric infections is crucial for prioritizing control strategies for foodborne and waterborne diseases.This study aimed to assess the global burden of enteric infections in 2021 and identify risk factors from One Health aspects.Methods:Leveraging the Global Burden of Disease(GBD)2021 database,the incidence,disability-adjusted life years(DALYs),and deaths of enteric infections and the subtypes were estimated,including diarrheal diseases,typhoid and paratyphoid fever,invasive non-typhoidal Salmonella(iNTS)infections,and other intestinal infectious diseases.The estimates were quantified by absolute number,age-standardized incidence rate(ASIR),agestandardized mortality rate(ASMR)and age-standardized DALY rate with 95%uncertainty intervals(UIs).Thirteen pathogens and three risk factors associated with diarrheal diseases were analyzed.Results:In 2021,the global age-standardized DALY rate of enteric infections was 1020.15 per 100,000 popultion(95%UI:822.70-1259.39 per 100,000 population)with an estimated annual percentage change(EAPC)of-4.11%(95%confidence interval:-4.31%to-3.90%)in 1990-2021.A larger burden was observed in regions with lower socio-demographic index(SDI)levels.Diarrheal disease was the most serious subtype with Western Sub-Saharan Africa exhibiting the highest age-standardized DALY rate(2769.81 per 100,000 population,95%UI:1976.80-3674.41 per 100,000 population).Children under 5 and adults over 65 years suffered more from diarrheal diseases with the former experiencing the highest global age-standardized DALY rate(9382.46 per 100,000 population,95%UI:6771.76-13,075.12 per 100,000 population).Rotavirus remained the leading cause of diarrheal diseases despite a cross-year decline in the observed age-standardized DALY rate.Unsafe water,sanitation,and handwashing contributed most to the disease burden.Conclusion:The reduced burden of enteric infections suggested the effectiveness of previous control strategies;however,more efforts should be made in vulnerable regions and populations through a One Health approach.
文摘Botulism is a severe neuroparalytic disease caused by Clostridium botulinums toxins.Although the disease is uncommon,causes great concern due to its high rate of mortality;foodbome outbreaks of botulism occur worldwide and require immediate public health and acute care resources.This study had a review of outstanding outbreaks published;Journals articles related to the subject.From the outbreaks analysis we found the most involved food products were;fermented fish products in Alaska;home canning food,oil preservation and restaurant sauce in London and USA;home canned vegetables,food airtight packed with inappropriate refrigeration and aerosols in Argentina.In conclusion,the diagnosis is based only on clinical findings matching the disease and previous exposure to suspicious food.Botulism must be immediately identified as one case suggests an epidemic and should be treated as a public health emergency.Therefore the purpose of the following review is to recognize the associated risks with the consumption of potentially dangerous foods,to help work our way on prevention for every public health professional to be aware of the dangers of this potentially lethal disease.
基金supported by the National Natural Science Foundation of China(11874021,61675072 and 21505047)the Science and Technology Project of Guangdong Province of China(2017A020215059)+2 种基金the Science and Technology Project of Guangzhou City(201904010323 and 2019050001)the Innovation Project of Graduate School of South China Normal University School(2019LKXM023)the Natural Science Research Project of Guangdong Food and Drug Vocational College(2019ZR01)
文摘Two-dimensional(2D)nanomaterials have captured an increasing attention in biophotonics owing to their excellent optical features.Herein,2D hafnium ditelluride(HfTe_(2)),a new member of transition metal tellurides,is exploited to support gold nanoparticles fabricating HfTe_(2)-Au nanocomposites.The nanohybrids can serve as novel 2D surface-enhanced Raman scattering(SERS)substrate for the label-free detection of analyte with high sensitivity and reproducibility.Chemical mechanism originated from HfTe_(2) nanosheets and the electromagnetic enhancement induced by the hot spots on the nano-hybrids may largely contribute to the superior SERS effect of HfTe_(2)-Au nanocomposites.Finally,HfTe_(2)-Au nanocomposites are utilized for the label-free SERS analysis of foodborne pathogenic bac-teria,which realize the rapid and ultrasensitive Raman test of Escherichia coli,Listeria mono-cytogenes,Staphylococcus aureus and Salmonella with the limit of detection of 10 CFU/mL and the maximum Raman enhancement factor up to 1.7×10^(8).Combined with principal component analysis,HfTe_(2)-Au-based SERS analysis also completes the bacterial classification without extra treatment.
文摘Infection and intoxication are two common types of foodborne illnesses throughout the world.The aim of the present work was to isolate and characterize biosurfactant producing bacteria fromgroundnut oil cake dumping sites and evaluate their biocontrol effects against foodborne pathogens.Bacteria were isolated by enrichment culture technique and preliminary screening method.Biosurfactant activity evaluation was carried out by oil displacement,drop collapse test,lipase activity,hemolytic activity,emulsification index and emulsification assay methods.16s rRNA sequence analysis was used for the isolate identification.Crude biosurfactant was extracted by acid precipitation method and characterized using FTIR(Fourier Transform Infrared Spectroscopy).Antibacterial activity was investigated using disc diffusion method.CMCand surface reduction were analyzed by DuNouy tensiometer.Top 10 strains were selected for biosurfactant activity assessment from the total 30 isolates.In addition,16s rRNA sequence identified that the potential isolate was Bacillus pseudomycoides OR 1.Then,FTIR result of the extracted biosurfactant established the extract as a lipopeptide based on the absorption peaks at 3500 to 3200 cm^-1 and 2963 to 2854.68 cm^-1,respectively.50μg/mL of lipopeptide showed the highest antibacterial activity.Critical Micelle Concentration(CMC)of the lipopeptide was 60 mg/L and it reduced the surface tension of water from 71.6 to 31.6 mN/m.Hence,this study widens the scope to employ the bacterial lipopeptide surfactant as a promising biocontrol agent against foodborne pathogens.
基金supported by Engineering Research Center of Bio-Process of Ministry of Educationsupport from Fundamental Research Funds for the Central Universities in China(PA2024GDSK0092,PA2025GDGP0026).
文摘In this work,to enhance the antibacterial potential of black rice anthocyanins for food preservation applications,cyanidin-3-O-glucoside(C3G)was isolated and subsequently acylated with capric acid using Novozym 435 lipase as the catalyst.The resulting derivative was identified as cyanidin-3-O-(6″-capryl)-glucoside(ACD).This enzy-matic modification successfully achieved its goal,as ACD exhibited significantly enhanced antibacterial activity against major foodborne pathogens.Its minimum inhibitory concentrations to Listeria monocytegenes,Staphylo-coccus aureus,and Vibrio parahaemolyticus were 50.00%-87.30%lower than those of the precursor C3G.After ACD treatment,the bacterial cells displayed twisted and sunken morphologies.The permeability of cell walls and membranes was severely compromised,as evidenced by 78.57%-346.43%increase in extracellular alkaline phosphatase activity and a 60%to 73.5%increase in nucleic acid leakage compared to untreated controls.Furthermore,ACD interfered with intracellular functions,reducing the activities of adenosine triphosphatase and superoxide dismutase by 4.58 U/mL and 4.88 U/mL,respectively,and inhibiting the tricarboxylic acid cycle by 85.2%.This study demonstrated that enzymatic acylation was an effective strategy to enhance the antibacterial efficacy of black rice anthocyanins through multi-target mechanisms,highlighting the potential of ACD as a promising candidate for development as a natural food preservative.
文摘The aim of this study was to investigate the antibacterial,antibiofilm,antioxidant and shelf life protective po-tentials of infusion,decoction,and room temperature samples of sumac fruits prepared as used by the local people against three foodborne pathogens including Bacillus cereus,Escherichia coli O157:H7,and Staphylococcus aureus isolated from food samples.The room temperature samples of sumac showed a remarkable antibacterial activity(81.25%)against S.aureus.Infusion and decoction samples of sumac fruit showed an effective anti-bacterial activity(~70%)against E.coli O157:H7.Moderate efficacy against B.cereus was observed in all treatment groups.Antibiofilm effects of sumac were observed against B.cereus and S.aureus,but not against E.coli O157:H7.The total antioxidant activity was found to be close to the reference compounds.The twenty-two phenolic and flavonoid chemical compounds(gallic acid,gentisic acid,protocatechuic acid,proto-catechualdehyde,4-OH-benzoic acid,salicylic acid,chlorogenic acid,cafeic acid,p-coumaric acid,ferulic acid,rosmarinic acid,sinapic acid,trans-cinnamic acid,rutin,resveratrol,myricetin,naringenin,quercetin,luteolin,kaempferol,apigenin,and chrysin)were identified by Liquid Chromatography Mass Spectrometer(LC-MS/MS)and these molecules were analyzed by computational analyses using SwissADME,admetSAR 3.0 and the AntiBac-Pred web tool.In addition,the antibacterial efficacy of sumac fruit extracts was tested on mixed Mediterranean salad samples to evaluate the shelf-life enhancing effect.The efficacy of sumac fruit against common foodborne pathogens suggests that the ethnobotanical use of sumac may provide protection against bacterial diseases and can be utilized as an additive and/or ingredient in the food industry.
文摘Ensuring food safety remains a global challenge because of the presence of foodborne pathogens,such as Escherichia coli and Staphylococcus aureus.In this study,we present a turn-on fluorescence biosensor based on an iron metal-organic framework(Fe-MOF)for the selective and rapid detection of two major foodborne pathogens,E.coli and S.aureus.Fe-MOF was synthesised via a solvothermal method and thoroughly characterised by Fourier-transform infrared spectroscopy(FTIR),X-ray diffraction(XRD),scanning electron microscopy with energy-dispersive X-ray spectroscopy(SEM-EDX),and thermogravimetric analysis(TGA).Upon interaction with bacterial suspensions,the sensor exhibited significant fluorescence enhancement,which was attributed to electron transfer modulation triggered by bacterial surface components.The analytical performance was evaluated across three matrices—phosphate-buffered saline(PBS),drinking water,and milk—achieving detection limits as low as 0.464 log CFU/mL for S.aureus and 0.584 log CFU/mL for E.coli.The sensor enabled rapid detection within less than 1 h with high recovery rates(95-104%)across all tested matrices,confirming its practical usability for real sample analysis.These findings address a critical gap in biosensor research by validating Fe-MOFs for practical food-safety applications in multiple real-world environments.
