AIM: To investigate the effect of polydatin (PD), a resveratrol glucoside, on mast cell degranulation and antiallergic activity. METHODS: After the rats were orally sensitized with ovalbumin (OVA) for 48 d and underwe...AIM: To investigate the effect of polydatin (PD), a resveratrol glucoside, on mast cell degranulation and antiallergic activity. METHODS: After the rats were orally sensitized with ovalbumin (OVA) for 48 d and underwent PD treatment for 4 d, all the rats were stimulated by 100 mg/mL OVA for24 h and then sacrificed for the following experiments. The small intestines from all the groups were prepared for morphology examination by hematoxylin and eosin staining. We also used a smooth muscle organ bath to evaluate the motility of the small intestines. The OVA-specific immunoglobulin E (IgE) production and interleu-kin-4 (IL-4) levels in serum or supernatant of intestinal mucosa homogenates were analyzed by enzyme-linked immunosorbent assay (ELISA). Using toluidine blue stain, the activation and degranulation of isolated rat peritoneal mast cells (RPMCs) were analyzed. Release of histamine from RPMCs was measured by ELISA, and regulation of PD on intracellular Ca 2+ mobilization was investigated by probing intracellular Ca 2+ with fluo-4 fluo-rescent dye, with the signal recorded and analyzed. RESULTS: We found that intragastric treatment with PD significantly reduced loss of mucosal barrier integrity in the small intestine. However, OVA-sensitization caused significant hyperactivity in the small intestine of allergic rats, which was attenuated by PD administration by 42% (1.26 ± 0.13 g vs OVA 2.18 ± 0.21 g, P < 0.01). PD therapy also inhibited IgE production (3.95 ± 0.53 ng/mL vs OVA 4.53 ± 0.52 ng/mL, P < 0.05) by suppressing the secretion of Th2-type cytokine, IL-4, by 34% (38.58 ± 4.41 pg/mLvs OVA 58.15 ± 6.24 pg/mL, P < 0.01). The ratio of degranulated mast cells, as indicated by vehicles (at least five) around the cells, dramatically increased in the OVA group by 5.5 fold (63.50% ± 15.51% vs phosphate-buffered saline 11.15% ± 8.26%, P < 0.001) and fell by 65% after PD treatment (21.95% ± 4.37% vs OVA 63.50% ± 15.51%, P < 0.001). PD mediated attenuation of mast cell degranulation was further confirmed by decreased histamine levels in both serum (5.98 ± 0.17 vs OVA 6.67 ± 0.12, P < 0.05) and intestinal mucosa homogenates (5.83 ± 0.91 vs OVA 7.35 ± 0.97, P < 0.05). Furthermore, we demonstrated that administration with PD significantly decreased mast cell degranulation due to reduced Ca 2+ influx through store-operated calcium channels (SOCs) (2.35 ± 0.39vs OVA 3.51 ± 0.38,P < 0.01).CONCLUSION: Taken together, our data indicate that PD stabilizes mast cells by suppressing intracellular Ca 2+ mobilization, mainly through inhibiting Ca 2+ entry via SOCs, thus exerting a protective role against OVA-sensitized food allergy.展开更多
By the food storing preference experiment conducted on the M. Brandti ,it is revealed that there is an apparent preference of this species: the most favorable component is the root of Artemisia scoparia ,then the A.fr...By the food storing preference experiment conducted on the M. Brandti ,it is revealed that there is an apparent preference of this species: the most favorable component is the root of Artemisia scoparia ,then the A.frigida ,and then the branch and fruit of A.scoparia .Such preference is related with the food storing order in the caches of the communal over winter group.展开更多
The family group food-storing-territories of Microtus brandti are analyzed in 4 different grazing habitations: light-grazing, middle-grazing, over-grazing, and serious-grazing. Significant differences have been detect...The family group food-storing-territories of Microtus brandti are analyzed in 4 different grazing habitations: light-grazing, middle-grazing, over-grazing, and serious-grazing. Significant differences have been detected in the food-storing-territory of M.brandti in various habitations with ANOVA. The food-storing-territory and vegetative biomass has exhibited an apparent negative correlation. This may be considered an adaptation of M.brandti to the variable environment.展开更多
Two methods were used to estimate the foodstoringterritory of communal overwinter nest of Brandt’s vole, that is, the rootcounting method and runwaymeasuring technique. It reveals that the 2 estimators accord with di...Two methods were used to estimate the foodstoringterritory of communal overwinter nest of Brandt’s vole, that is, the rootcounting method and runwaymeasuring technique. It reveals that the 2 estimators accord with direct proportion relationship. The utility rate of the group on its food storing territory is about 67 percent. The runway measuring method may be considered as a convenient estimator of the food storing territory of communal over winter group of this展开更多
基金Supported by The Natural Science Foundation of China,No.81271950,to Ji QMProjects of International/HMT(Hong Kong,Macao,and Taiwan)Cooperation and Innovation Platform in Science and Technology of Guangdong Higher Education Institutions,No.2012gjhz0009,to Liu ZG+2 种基金Key Laboratory Construction Program of Shenzhen,No.SW201110010,to Liu ZGBasic Research Program of Shenzhen University,No.201101,to Liu ZGBasic Research Foundation of Shenzhen,No.JC201005250059A,JCYJ20120613115535998
文摘AIM: To investigate the effect of polydatin (PD), a resveratrol glucoside, on mast cell degranulation and antiallergic activity. METHODS: After the rats were orally sensitized with ovalbumin (OVA) for 48 d and underwent PD treatment for 4 d, all the rats were stimulated by 100 mg/mL OVA for24 h and then sacrificed for the following experiments. The small intestines from all the groups were prepared for morphology examination by hematoxylin and eosin staining. We also used a smooth muscle organ bath to evaluate the motility of the small intestines. The OVA-specific immunoglobulin E (IgE) production and interleu-kin-4 (IL-4) levels in serum or supernatant of intestinal mucosa homogenates were analyzed by enzyme-linked immunosorbent assay (ELISA). Using toluidine blue stain, the activation and degranulation of isolated rat peritoneal mast cells (RPMCs) were analyzed. Release of histamine from RPMCs was measured by ELISA, and regulation of PD on intracellular Ca 2+ mobilization was investigated by probing intracellular Ca 2+ with fluo-4 fluo-rescent dye, with the signal recorded and analyzed. RESULTS: We found that intragastric treatment with PD significantly reduced loss of mucosal barrier integrity in the small intestine. However, OVA-sensitization caused significant hyperactivity in the small intestine of allergic rats, which was attenuated by PD administration by 42% (1.26 ± 0.13 g vs OVA 2.18 ± 0.21 g, P < 0.01). PD therapy also inhibited IgE production (3.95 ± 0.53 ng/mL vs OVA 4.53 ± 0.52 ng/mL, P < 0.05) by suppressing the secretion of Th2-type cytokine, IL-4, by 34% (38.58 ± 4.41 pg/mLvs OVA 58.15 ± 6.24 pg/mL, P < 0.01). The ratio of degranulated mast cells, as indicated by vehicles (at least five) around the cells, dramatically increased in the OVA group by 5.5 fold (63.50% ± 15.51% vs phosphate-buffered saline 11.15% ± 8.26%, P < 0.001) and fell by 65% after PD treatment (21.95% ± 4.37% vs OVA 63.50% ± 15.51%, P < 0.001). PD mediated attenuation of mast cell degranulation was further confirmed by decreased histamine levels in both serum (5.98 ± 0.17 vs OVA 6.67 ± 0.12, P < 0.05) and intestinal mucosa homogenates (5.83 ± 0.91 vs OVA 7.35 ± 0.97, P < 0.05). Furthermore, we demonstrated that administration with PD significantly decreased mast cell degranulation due to reduced Ca 2+ influx through store-operated calcium channels (SOCs) (2.35 ± 0.39vs OVA 3.51 ± 0.38,P < 0.01).CONCLUSION: Taken together, our data indicate that PD stabilizes mast cells by suppressing intracellular Ca 2+ mobilization, mainly through inhibiting Ca 2+ entry via SOCs, thus exerting a protective role against OVA-sensitized food allergy.
文摘By the food storing preference experiment conducted on the M. Brandti ,it is revealed that there is an apparent preference of this species: the most favorable component is the root of Artemisia scoparia ,then the A.frigida ,and then the branch and fruit of A.scoparia .Such preference is related with the food storing order in the caches of the communal over winter group.
文摘The family group food-storing-territories of Microtus brandti are analyzed in 4 different grazing habitations: light-grazing, middle-grazing, over-grazing, and serious-grazing. Significant differences have been detected in the food-storing-territory of M.brandti in various habitations with ANOVA. The food-storing-territory and vegetative biomass has exhibited an apparent negative correlation. This may be considered an adaptation of M.brandti to the variable environment.
文摘Two methods were used to estimate the foodstoringterritory of communal overwinter nest of Brandt’s vole, that is, the rootcounting method and runwaymeasuring technique. It reveals that the 2 estimators accord with direct proportion relationship. The utility rate of the group on its food storing territory is about 67 percent. The runway measuring method may be considered as a convenient estimator of the food storing territory of communal over winter group of this
