Objective: To observe the neuroprotective mechanism of water extract of Fomito^p^is Pinicola on MPP+ induced apoptosis of mesencephala dopaminergic cells in vitro. Methods: The antioxidant activity of fungi was determ...Objective: To observe the neuroprotective mechanism of water extract of Fomito^p^is Pinicola on MPP+ induced apoptosis of mesencephala dopaminergic cells in vitro. Methods: The antioxidant activity of fungi was determined by FRAP method. The anti-inflammatory activity of the fungi was detected by LPS-induced NO release method. Mesencephalic dopaminergic neurons were labeled by TH staining to observe the survival of THir neurons. Results: In the anti-oxidant activity assay, the Trolox equivalent anti-oxidant capacity (TEAC) of water extract of Fomitopsis Pinicola was determined to be ( 165.80±7.13 )μmol Trolox/g extract. Water extracts o f Fomitopsis Pinicola treatment(100, 5 0 ,2 5 , 12.5^g/ml) decreased NO formation significantly. MPP+ induced significant chromatin condensation in the nuclei of mesencephala dopaminergic neurons with nuclear lysis, the mitochondrial membrane potential decreased remarkably, and ROS production increased significantly. Compared with the MPP+ control group, the morphological changes of cell nuclei after apoptosis was reversed by water extract of Fomitopsis Pinicola. Water extract of Fomitopsis Pinicola treatment (50,25,12.5^g/ml) dramatically increased relative mitochondrial membrane potential compared with MPP+ control respectively. Compared with the MPP+ control, water extract of Fomitopsis Pinicola treatment (50, 25^g/ml) significantly decreased relative ROS formation respectively. Conclusions: Water extract of Fomitopsis Pinicola showed significant neuroprotective effect on mesencephala dopaminergic cells induced by MPP+. The water extract of Fomitopsis Pinicola showed antioxidant and anti-inflammatory activities. The mechanism of neuroprotective effect of water extract of Fomitopsis Pinicola may be related to inhibitory on mitochondrial oxidative stress.展开更多
Six triphenylmethane dyes viz., bromophenol blue, basic fuchsin, methyl violet, methyl green, ethyl violet and malachite green were studied for their decolorization by Fomitopsis feei. Among, basic fuchsin (98%) was m...Six triphenylmethane dyes viz., bromophenol blue, basic fuchsin, methyl violet, methyl green, ethyl violet and malachite green were studied for their decolorization by Fomitopsis feei. Among, basic fuchsin (98%) was maximum decolorized followed by bromophenol blue (96.8%). However, the rate of bromophenol blue decolorization was very fast. There was no correlation between the lignolytic activity and dye decolurization of the dyes. The highest laccase and lignin peroxidase activities were observed in basic fuchsin (46 U/mL) and methyl green (44 U/mL) respectively after 21 days of incubation, which were poor dye degraders. The triphenylmethane reductase enzyme was the responsible enzyme for the decolorization of these triphenyl methane dyes. The treatment by using fungal organisms was considered to be the cost-effective and ecofriendly method of decolourization of effluents discharged from the dye industries.展开更多
文摘Objective: To observe the neuroprotective mechanism of water extract of Fomito^p^is Pinicola on MPP+ induced apoptosis of mesencephala dopaminergic cells in vitro. Methods: The antioxidant activity of fungi was determined by FRAP method. The anti-inflammatory activity of the fungi was detected by LPS-induced NO release method. Mesencephalic dopaminergic neurons were labeled by TH staining to observe the survival of THir neurons. Results: In the anti-oxidant activity assay, the Trolox equivalent anti-oxidant capacity (TEAC) of water extract of Fomitopsis Pinicola was determined to be ( 165.80±7.13 )μmol Trolox/g extract. Water extracts o f Fomitopsis Pinicola treatment(100, 5 0 ,2 5 , 12.5^g/ml) decreased NO formation significantly. MPP+ induced significant chromatin condensation in the nuclei of mesencephala dopaminergic neurons with nuclear lysis, the mitochondrial membrane potential decreased remarkably, and ROS production increased significantly. Compared with the MPP+ control group, the morphological changes of cell nuclei after apoptosis was reversed by water extract of Fomitopsis Pinicola. Water extract of Fomitopsis Pinicola treatment (50,25,12.5^g/ml) dramatically increased relative mitochondrial membrane potential compared with MPP+ control respectively. Compared with the MPP+ control, water extract of Fomitopsis Pinicola treatment (50, 25^g/ml) significantly decreased relative ROS formation respectively. Conclusions: Water extract of Fomitopsis Pinicola showed significant neuroprotective effect on mesencephala dopaminergic cells induced by MPP+. The water extract of Fomitopsis Pinicola showed antioxidant and anti-inflammatory activities. The mechanism of neuroprotective effect of water extract of Fomitopsis Pinicola may be related to inhibitory on mitochondrial oxidative stress.
文摘Six triphenylmethane dyes viz., bromophenol blue, basic fuchsin, methyl violet, methyl green, ethyl violet and malachite green were studied for their decolorization by Fomitopsis feei. Among, basic fuchsin (98%) was maximum decolorized followed by bromophenol blue (96.8%). However, the rate of bromophenol blue decolorization was very fast. There was no correlation between the lignolytic activity and dye decolurization of the dyes. The highest laccase and lignin peroxidase activities were observed in basic fuchsin (46 U/mL) and methyl green (44 U/mL) respectively after 21 days of incubation, which were poor dye degraders. The triphenylmethane reductase enzyme was the responsible enzyme for the decolorization of these triphenyl methane dyes. The treatment by using fungal organisms was considered to be the cost-effective and ecofriendly method of decolourization of effluents discharged from the dye industries.
