[Objectives] This study aimed to study the morphological characteristics and molecular phylogeny of a new strain collected from rotten wood of sea buckthorn in Yanbei area of Shanxi Province. [Methods]The specimens we...[Objectives] This study aimed to study the morphological characteristics and molecular phylogeny of a new strain collected from rotten wood of sea buckthorn in Yanbei area of Shanxi Province. [Methods]The specimens were observed with naked eyes. With Melzer,cotton blue and 5% KOH as floating carriers,the slices were prepared,which were observed under a microscope. The ITS sequence of the new strain was compared with those of the 20 kinds of reported fungi to construct molecular phylogenetic tree based on ITS. [Results] The new strain has horseshoe-shaped basidiocarp and pileus shape and has no stipe. Sometimes,it grows procumbently and scrolled backward. The mycelial system is monomitic. The skeletal hyphae showed a negative reaction in the Melzer reagent and cotton blue reagent. The basidiospores are subglobose or spherical and colorless with thick wall. The results of molecular phylogenetic analysis showed that the new strain had a close relationship with Fomitiporia spp. and was clustered into the same branch with them. It formed an independent sub-branch in the phylogenetic tree. [Conclusions] The new strain was identified as a new species of Fomitiporia,named as Fomitiporia yanbeiensis S. Guo & L. Zhou( Gen Bank: KT861405,Fungal name: FN570360).展开更多
基金Supported by Coal-Based Key Science and Technology Program of Shanxi Province(FT2014-03)
文摘[Objectives] This study aimed to study the morphological characteristics and molecular phylogeny of a new strain collected from rotten wood of sea buckthorn in Yanbei area of Shanxi Province. [Methods]The specimens were observed with naked eyes. With Melzer,cotton blue and 5% KOH as floating carriers,the slices were prepared,which were observed under a microscope. The ITS sequence of the new strain was compared with those of the 20 kinds of reported fungi to construct molecular phylogenetic tree based on ITS. [Results] The new strain has horseshoe-shaped basidiocarp and pileus shape and has no stipe. Sometimes,it grows procumbently and scrolled backward. The mycelial system is monomitic. The skeletal hyphae showed a negative reaction in the Melzer reagent and cotton blue reagent. The basidiospores are subglobose or spherical and colorless with thick wall. The results of molecular phylogenetic analysis showed that the new strain had a close relationship with Fomitiporia spp. and was clustered into the same branch with them. It formed an independent sub-branch in the phylogenetic tree. [Conclusions] The new strain was identified as a new species of Fomitiporia,named as Fomitiporia yanbeiensis S. Guo & L. Zhou( Gen Bank: KT861405,Fungal name: FN570360).
