Effect of folic acid on delivery of liposomes was examined using cultured HeL.a cells.Folate-Abstractliposomes encapsulating calcein and liposomes were cultured with HeLa cells respectively.Liposomes taken up bycells ...Effect of folic acid on delivery of liposomes was examined using cultured HeL.a cells.Folate-Abstractliposomes encapsulating calcein and liposomes were cultured with HeLa cells respectively.Liposomes taken up bycells were measured by a fluorescence spectrophotometer.The cell fluorescence increased slowly at the 4 h timepoint,and the saturated folate-liposome concentration was 0.45 mg·mL'1.The binding of folate-liposomes to HeLacells could be competitively inhibited by excess free folate.Enzyme phospholipase D(PL.D)or phosphatidylinositol-specific phospholipase C(PIPLC)influenced liposome uptake via folate receptor.Folate targeting improved thespecificity of liposomes for cells which overexpressed the folate receptors.展开更多
基金Project supported by the National Natural Science Foundation of China(No 39670859)by the Research Fund for the Doctoral Program of Higher Education No 9506183.
文摘Effect of folic acid on delivery of liposomes was examined using cultured HeL.a cells.Folate-Abstractliposomes encapsulating calcein and liposomes were cultured with HeLa cells respectively.Liposomes taken up bycells were measured by a fluorescence spectrophotometer.The cell fluorescence increased slowly at the 4 h timepoint,and the saturated folate-liposome concentration was 0.45 mg·mL'1.The binding of folate-liposomes to HeLacells could be competitively inhibited by excess free folate.Enzyme phospholipase D(PL.D)or phosphatidylinositol-specific phospholipase C(PIPLC)influenced liposome uptake via folate receptor.Folate targeting improved thespecificity of liposomes for cells which overexpressed the folate receptors.
