Background: Programmed cell death protein 4(PDCD4) is a novel tumor suppressor protein involved in pro?grammed cell death. Its association with cancer progression has been observed in multiple tumor models, but eviden...Background: Programmed cell death protein 4(PDCD4) is a novel tumor suppressor protein involved in pro?grammed cell death. Its association with cancer progression has been observed in multiple tumor models, but evidence supporting its association with solid tumors in humans remains controversial. This study aimed to determine the clinical signiicance and prognostic value of PDCD4 in solid tumors.Methods: A systematic literature review was performed to retrieve publications with available clinical informa?tion and survival data. The eligibility of the selected articles was based on the criteria of the Dutch Cochrane Centre proposed by the Meta?analysis Of Observational Studies in Epidemiology group. Pooled odds ratios(ORs), hazard ratios(HRs), and 95% conidence intervals(CIs) for survival analysis were calculated. Publication bias was examined by Begg's and Egger's tests.Results: Clinical data of 2227 cancer patients with solid tumors from 23 studies were evaluated. PDCD4 expression was signiicantly associated with the diferentiation status of head and neck cancer(OR 4.25, 95% CI 1.87–9.66) and digestive system cancer(OR 2.87, 95% CI 1.84–4.48). Down?regulation of PDCD4 was signiicantly associated with short overall survival of patients with head and neck(HR: 3.44, 95% CI 2.38–4.98), breast(HR: 1.86, 95% CI 1.36–2.54), digestive system(HR: 2.12, 95% CI 1.75–2.56), and urinary system cancers(HR: 3.16, 95% CI 1.06–9.41).Conclusions: The current evidence suggests that PDCD4 down?regulation is involved in the progression of several types of solid tumor and is a potential marker for solid tumor prognoses. Its clinical usefulness should be conirmed by large?scale prospective studies.展开更多
目的分析首发精神分裂症患者认知水平与血清高迁移率族蛋白B1(high mobil ity group protein B1,HMGB1)、胃饥饿素(Ghrelin)、程序性细胞死亡因子4(programmed cell death factor 4,PDCD4)的相关性。方法选定乌鲁木齐市第四人民医院2021...目的分析首发精神分裂症患者认知水平与血清高迁移率族蛋白B1(high mobil ity group protein B1,HMGB1)、胃饥饿素(Ghrelin)、程序性细胞死亡因子4(programmed cell death factor 4,PDCD4)的相关性。方法选定乌鲁木齐市第四人民医院2021年6月~2023年6月就诊的60例首发精神分裂症患者为研究对象,以MATRICS公认认知成套测验(MATRICS consensus cognitive battery,MCCB)量表对患者进行认知功能评价,以MCCB量表评价患者认知功能并分组,比较认知功能正常组(39例)、认知功能障碍组(21例)阳性阴性症状评定量表(PANSS)评分、血清HMGB1、Ghrelin、PDCD4水平的差异,Pearson分析血清HMGB1、Ghrelin、PDCD4与MCCB评分、PANSS评分的相关性。结果认知功能障碍组MCCB评分、血清Ghrelin、PDCD4水平均低于认知功能正常组(P<0.05);认知功能障碍组PANSS评分、血清HMGB1均高于认知功能正常组(P<0.05);血清HMGB1与MCCB评分呈负相关性(P<0.05,r值=-0.394),与PANSS评分呈正相关性(P<0.05,r值=0.405),血清Ghrelin、PDCD4与MCCB评分均呈正相关性(P均<0.05,r值=0.386、0.412),血清Ghrelin、PDCD4与PANSS评分均呈负相关性(P<0.05,r值=-0.392、-0.402)。结论首发精神分裂症患者血清Ghrelin、PDCD4水平较低,血清HMGB1水平较高,血清HMGB1、Ghrelin、PDCD4与首发精神分裂症患者认知功能具有相关性。展开更多
Peking Union Medical College(PUMC)launched the"4+4"Medical Doctor(MD)pilot program in 2018,admitting students with non-medical backgrounds from top universities,aligning with national medical talent training...Peking Union Medical College(PUMC)launched the"4+4"Medical Doctor(MD)pilot program in 2018,admitting students with non-medical backgrounds from top universities,aligning with national medical talent training policies to foster diverse and eager learners in medicine.On the occasion of the graduation of the first class of the"4+4"MD pilot class at PUMC in 2023,we reviewed the teaching reform in the pilot program and carried out a systematic survey and interviews with students,faculties,and management staff of the pilot class.This article reports on the measures taken by the pilot class at PUMC in enrollment and curriculum setting,and demonstrates the achievements of the pilot class in terms of student academic background structure,knowledge acquisition and skill learning,scientific research ability,and course evaluation.The results indicated that the pilot class had met the national demand for the"Medicine+X"talent training model.More specifically,with a diverse academic backgrounds,the pilot class graduates had academic levels comparable to the eight-year medical education graduates,and their scientific research abilities were satisfactory.The pilot program at PUMC will optimize the curriculum setting,strengthen the construction of faculty,learning resources,and teaching facilities,and reform the academic evaluation methods,thus deepening the reform of medical education and improving the"4+4"MD program as a novel medical education model.展开更多
AIM:To investigate the expression of programmed cell death 4(Pdcd4)tumor suppressor gene in tissue specimen of renal cell carcinoma(RCC),testicular germ cell cancer and penile cancer.METHODS:Pdcd4 expression was studi...AIM:To investigate the expression of programmed cell death 4(Pdcd4)tumor suppressor gene in tissue specimen of renal cell carcinoma(RCC),testicular germ cell cancer and penile cancer.METHODS:Pdcd4 expression was studied using immunohistochemistry in 188 cases of RCC and 28 controls(including 9 oncocytoma);in 74 cases of penile carcinoma(including 17 metastatic tissue samples)and26 controls;in 11 cases of seminoma,in 14 cases of non-seminoma and 5 controls.RESULTS:Control tissues exhibited strong core and cytoplasmatic Pdcd4 staining.In contrast,core and cy-toplasmatic Pdcd4 levels were significantly decreased in cancer tissues.CONCLUSION:Our data support a role for Pdcd4(down-)regulation in urologic tumors.Interestingly,Pdcd4 expression seem to be a potential diagnostic marker for renal or penile tumors.展开更多
Objective:To explore the diagnostic value of PDCD4 on the degree of arterial stenosis in"blood stasis"coronary heart disease.Methods:Select 80 patients with coronary heart disease in the Second Cardiovascula...Objective:To explore the diagnostic value of PDCD4 on the degree of arterial stenosis in"blood stasis"coronary heart disease.Methods:Select 80 patients with coronary heart disease in the Second Cardiovascular Zone of the First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine in April 2020,and divide them into the"phlegm toxin"group(n=40)and the"phlegm stasis"group(n=40)based on the dialectics of traditional Chinese medicine.).Record the gender,age,smoking,and alcohol consumption of the subjects between the two groups,and detect their white blood cell count,neutrophil count,platelet count,platelet volume,platelet distribution width,blood creatinine,uric acid,cystatin,and fibrin The expression levels of original,high-sensitivity C-reactive protein,D-dimer,total cholesterol,triglycerides,high-density lipoprotein,low-density lipoprotein,apolipoprotein a,apolipoprotein b,and PDCD4.Multivariate logistic regression analysis was used to screen out the risk factors that affect coronary plaque formation,and the receiver operating characteristic(ROC)curve of each index was established to evaluate the severity of coronary stenosis in patients with stasis coronary heart disease by each index and combined index Diagnostic efficiency.Results:The two groups of patients were tested in terms of gender,age,smoking,drinking,triglycerides,cholesterol,high-density lipoprotein,low-density lipoprotein,apolipoprotein-a,apolipoprotein-b,white blood cell count,neutrophil The cell count,platelet count,platelet volume width and platelet distribution width were not statistically significant(P>0.05);the expression levels of hypersensitivity-C-reactive protein,serum creatinine,cystatin,uric acid and PDCD4 were statistically significant between the two groups Difference(P<0.05),and the corresponding hypersensitivity-C-reactive protein,creatinine,cystatin,uric acid and PDCD4 expression levels in the blood stasis group were higher than those in the phlegm blood stasis group.After multivariate logistic regression analysis,the level of PDCD4 in peripheral blood[OR=31.088,95%CI(2.498,3.869)]was an independent influencing factor of the"stagnation"type of coronary heart disease,and PDCD4 was diagnosed as the"stagnation"type of coronary heart disease The area under the ROC curve(AUC)is 88.6%,95%CI(1.894,2.532)(P=0.29);the level of PDCD4 in peripheral blood is positively correlated with the number and severity of coronary artery disease,the number of coronary artery disease and stenosis The greater the degree,the higher the detection value of PDCD4,(P<0.05).Conclusion:The expression level of PDCD4 in peripheral blood is closely related to the subtype of"stasis toxin"and the severity of coronary vascular stenosis.It can be used as a quantitative diagnostic index for the diagnosis of"stasis toxin"coronary heart disease and the severity of coronary vascular stenosis.展开更多
目的探索肝细胞癌(HCC)患者外周血程序性细胞死亡受体1(programmed death-1,PD-1)、细胞毒T淋巴细胞相关抗原4(cytotoxic T lymphocyte-associated antigen-4,CTLA-4)、Th1/Th2及其相关细胞因子水平,并分析其与肿瘤标志物甲胎蛋白(alpha...目的探索肝细胞癌(HCC)患者外周血程序性细胞死亡受体1(programmed death-1,PD-1)、细胞毒T淋巴细胞相关抗原4(cytotoxic T lymphocyte-associated antigen-4,CTLA-4)、Th1/Th2及其相关细胞因子水平,并分析其与肿瘤标志物甲胎蛋白(alpha fetoprotein,AFP)、异常凝血酶原(protein induced by vitamin K absence or antagonistⅡ,PIVKAⅡ)的相关性。方法选择2021年1月至2022年12月简阳市中医医院收治的50例HCC患者作为HCC组,选择同期行健康体检者作为对照组。采用酶联免疫吸附法测定测定外周血AFP、PIVKAⅡ、CTLA-4、PD-1、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、γ干扰素(interferonγ,IFN-γ)、白细胞介素-6(interleukin-6,IL-6)和白细胞介素-10(interleukin-10,IL-10)的水平。采用酶联免疫斑点法测定外周血Th1/Th2。比较两组患者上述指标差异,并采用Pearson法将上述指标与HCC组患者AFP和PIVKAⅡ水平进行相关性分析。结果与对照组相比,HCC组患者外周血TNF-α、IFN-γ及Th1/Th2细胞比例明显下降,而AFP、PIVKAⅡ、IL-6、IL-10、CTLA-4和PD-1水平则明显升高,差异有统计学意义(P<0.05)。相关性分析表明,Th1/Th2与AFP和PIVKA负相关性最高,而PD-1与AFP和PIVKA正相关性最高(r>|7|,P<0.05)。结论HCC患者外周血Th1/Th2明显降低,而CTLA-4和PD-1显著升高,且与HCC血清肿瘤标志物AFP和PIVKA具有密切关系,基于上述指标可为HCC的临床诊断和免疫治疗评估提供参考依据。展开更多
4D printing technology represents a new generation of additive manufacturing methods that enable three-dimensional(3D)printed structures to change their shapes or properties over time(the fourth dimension)in response ...4D printing technology represents a new generation of additive manufacturing methods that enable three-dimensional(3D)printed structures to change their shapes or properties over time(the fourth dimension)in response to external stimuli such as temperature,magnetic fields,and light.Among the most popular types of 4D-printed structures are thermally responsive bilayer actuators using shape memory polymers,valued for their programmability and convenience.However,achieving precise deformations without collisions is hindered by the nonlinear and time-varying morphing process of these bilayer actuators,which is crucial for creating dynamically controllable shapes on demand in 4D printing.This study presents a rapid and effective design and optimization strategy for 4D printed self-folding structures that can be sequentially and accurately folded.Theoretical analyses were conducted to guide the design of the folding processes.The response surface method(RSM)was used to investigate key parameters affecting the design of 4D-printed bilayer actuators.The results indicate that increasing printing speed enhances internal strain,whereas higher printing temperatures,layer heights,or actuator heights have the opposite effect.The RSM model achieved an R-squared value of 0.983,accurately capturing the coupling effects of these variables on the output responses,thereby enabling controlled timescales for bending motion and sequential folding without collisions.These findings can be applied to enhance the design and acceleration of 4D-printed self-folding structures,ensuring controlled speed of shape transformation.To validate this concept,a self-folding hand-shaped structure with five fingers was designed and fabricated,demonstrating how design and printing parameters can precisely control the timescale of shape changes for each finger based on the design specifications.展开更多
文摘Background: Programmed cell death protein 4(PDCD4) is a novel tumor suppressor protein involved in pro?grammed cell death. Its association with cancer progression has been observed in multiple tumor models, but evidence supporting its association with solid tumors in humans remains controversial. This study aimed to determine the clinical signiicance and prognostic value of PDCD4 in solid tumors.Methods: A systematic literature review was performed to retrieve publications with available clinical informa?tion and survival data. The eligibility of the selected articles was based on the criteria of the Dutch Cochrane Centre proposed by the Meta?analysis Of Observational Studies in Epidemiology group. Pooled odds ratios(ORs), hazard ratios(HRs), and 95% conidence intervals(CIs) for survival analysis were calculated. Publication bias was examined by Begg's and Egger's tests.Results: Clinical data of 2227 cancer patients with solid tumors from 23 studies were evaluated. PDCD4 expression was signiicantly associated with the diferentiation status of head and neck cancer(OR 4.25, 95% CI 1.87–9.66) and digestive system cancer(OR 2.87, 95% CI 1.84–4.48). Down?regulation of PDCD4 was signiicantly associated with short overall survival of patients with head and neck(HR: 3.44, 95% CI 2.38–4.98), breast(HR: 1.86, 95% CI 1.36–2.54), digestive system(HR: 2.12, 95% CI 1.75–2.56), and urinary system cancers(HR: 3.16, 95% CI 1.06–9.41).Conclusions: The current evidence suggests that PDCD4 down?regulation is involved in the progression of several types of solid tumor and is a potential marker for solid tumor prognoses. Its clinical usefulness should be conirmed by large?scale prospective studies.
文摘目的分析首发精神分裂症患者认知水平与血清高迁移率族蛋白B1(high mobil ity group protein B1,HMGB1)、胃饥饿素(Ghrelin)、程序性细胞死亡因子4(programmed cell death factor 4,PDCD4)的相关性。方法选定乌鲁木齐市第四人民医院2021年6月~2023年6月就诊的60例首发精神分裂症患者为研究对象,以MATRICS公认认知成套测验(MATRICS consensus cognitive battery,MCCB)量表对患者进行认知功能评价,以MCCB量表评价患者认知功能并分组,比较认知功能正常组(39例)、认知功能障碍组(21例)阳性阴性症状评定量表(PANSS)评分、血清HMGB1、Ghrelin、PDCD4水平的差异,Pearson分析血清HMGB1、Ghrelin、PDCD4与MCCB评分、PANSS评分的相关性。结果认知功能障碍组MCCB评分、血清Ghrelin、PDCD4水平均低于认知功能正常组(P<0.05);认知功能障碍组PANSS评分、血清HMGB1均高于认知功能正常组(P<0.05);血清HMGB1与MCCB评分呈负相关性(P<0.05,r值=-0.394),与PANSS评分呈正相关性(P<0.05,r值=0.405),血清Ghrelin、PDCD4与MCCB评分均呈正相关性(P均<0.05,r值=0.386、0.412),血清Ghrelin、PDCD4与PANSS评分均呈负相关性(P<0.05,r值=-0.392、-0.402)。结论首发精神分裂症患者血清Ghrelin、PDCD4水平较低,血清HMGB1水平较高,血清HMGB1、Ghrelin、PDCD4与首发精神分裂症患者认知功能具有相关性。
文摘Peking Union Medical College(PUMC)launched the"4+4"Medical Doctor(MD)pilot program in 2018,admitting students with non-medical backgrounds from top universities,aligning with national medical talent training policies to foster diverse and eager learners in medicine.On the occasion of the graduation of the first class of the"4+4"MD pilot class at PUMC in 2023,we reviewed the teaching reform in the pilot program and carried out a systematic survey and interviews with students,faculties,and management staff of the pilot class.This article reports on the measures taken by the pilot class at PUMC in enrollment and curriculum setting,and demonstrates the achievements of the pilot class in terms of student academic background structure,knowledge acquisition and skill learning,scientific research ability,and course evaluation.The results indicated that the pilot class had met the national demand for the"Medicine+X"talent training model.More specifically,with a diverse academic backgrounds,the pilot class graduates had academic levels comparable to the eight-year medical education graduates,and their scientific research abilities were satisfactory.The pilot program at PUMC will optimize the curriculum setting,strengthen the construction of faculty,learning resources,and teaching facilities,and reform the academic evaluation methods,thus deepening the reform of medical education and improving the"4+4"MD program as a novel medical education model.
文摘AIM:To investigate the expression of programmed cell death 4(Pdcd4)tumor suppressor gene in tissue specimen of renal cell carcinoma(RCC),testicular germ cell cancer and penile cancer.METHODS:Pdcd4 expression was studied using immunohistochemistry in 188 cases of RCC and 28 controls(including 9 oncocytoma);in 74 cases of penile carcinoma(including 17 metastatic tissue samples)and26 controls;in 11 cases of seminoma,in 14 cases of non-seminoma and 5 controls.RESULTS:Control tissues exhibited strong core and cytoplasmatic Pdcd4 staining.In contrast,core and cy-toplasmatic Pdcd4 levels were significantly decreased in cancer tissues.CONCLUSION:Our data support a role for Pdcd4(down-)regulation in urologic tumors.Interestingly,Pdcd4 expression seem to be a potential diagnostic marker for renal or penile tumors.
基金Regional Fund Project of National Natural Science Foundation of China(No.8196086181460712)+1 种基金Guangxi Science Key Research and Development Program(No.AB19110006)Guangxi Support Project for Graduate Education Innovation(No.YCXJ2021052)。
文摘Objective:To explore the diagnostic value of PDCD4 on the degree of arterial stenosis in"blood stasis"coronary heart disease.Methods:Select 80 patients with coronary heart disease in the Second Cardiovascular Zone of the First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine in April 2020,and divide them into the"phlegm toxin"group(n=40)and the"phlegm stasis"group(n=40)based on the dialectics of traditional Chinese medicine.).Record the gender,age,smoking,and alcohol consumption of the subjects between the two groups,and detect their white blood cell count,neutrophil count,platelet count,platelet volume,platelet distribution width,blood creatinine,uric acid,cystatin,and fibrin The expression levels of original,high-sensitivity C-reactive protein,D-dimer,total cholesterol,triglycerides,high-density lipoprotein,low-density lipoprotein,apolipoprotein a,apolipoprotein b,and PDCD4.Multivariate logistic regression analysis was used to screen out the risk factors that affect coronary plaque formation,and the receiver operating characteristic(ROC)curve of each index was established to evaluate the severity of coronary stenosis in patients with stasis coronary heart disease by each index and combined index Diagnostic efficiency.Results:The two groups of patients were tested in terms of gender,age,smoking,drinking,triglycerides,cholesterol,high-density lipoprotein,low-density lipoprotein,apolipoprotein-a,apolipoprotein-b,white blood cell count,neutrophil The cell count,platelet count,platelet volume width and platelet distribution width were not statistically significant(P>0.05);the expression levels of hypersensitivity-C-reactive protein,serum creatinine,cystatin,uric acid and PDCD4 were statistically significant between the two groups Difference(P<0.05),and the corresponding hypersensitivity-C-reactive protein,creatinine,cystatin,uric acid and PDCD4 expression levels in the blood stasis group were higher than those in the phlegm blood stasis group.After multivariate logistic regression analysis,the level of PDCD4 in peripheral blood[OR=31.088,95%CI(2.498,3.869)]was an independent influencing factor of the"stagnation"type of coronary heart disease,and PDCD4 was diagnosed as the"stagnation"type of coronary heart disease The area under the ROC curve(AUC)is 88.6%,95%CI(1.894,2.532)(P=0.29);the level of PDCD4 in peripheral blood is positively correlated with the number and severity of coronary artery disease,the number of coronary artery disease and stenosis The greater the degree,the higher the detection value of PDCD4,(P<0.05).Conclusion:The expression level of PDCD4 in peripheral blood is closely related to the subtype of"stasis toxin"and the severity of coronary vascular stenosis.It can be used as a quantitative diagnostic index for the diagnosis of"stasis toxin"coronary heart disease and the severity of coronary vascular stenosis.
文摘目的探索肝细胞癌(HCC)患者外周血程序性细胞死亡受体1(programmed death-1,PD-1)、细胞毒T淋巴细胞相关抗原4(cytotoxic T lymphocyte-associated antigen-4,CTLA-4)、Th1/Th2及其相关细胞因子水平,并分析其与肿瘤标志物甲胎蛋白(alpha fetoprotein,AFP)、异常凝血酶原(protein induced by vitamin K absence or antagonistⅡ,PIVKAⅡ)的相关性。方法选择2021年1月至2022年12月简阳市中医医院收治的50例HCC患者作为HCC组,选择同期行健康体检者作为对照组。采用酶联免疫吸附法测定测定外周血AFP、PIVKAⅡ、CTLA-4、PD-1、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、γ干扰素(interferonγ,IFN-γ)、白细胞介素-6(interleukin-6,IL-6)和白细胞介素-10(interleukin-10,IL-10)的水平。采用酶联免疫斑点法测定外周血Th1/Th2。比较两组患者上述指标差异,并采用Pearson法将上述指标与HCC组患者AFP和PIVKAⅡ水平进行相关性分析。结果与对照组相比,HCC组患者外周血TNF-α、IFN-γ及Th1/Th2细胞比例明显下降,而AFP、PIVKAⅡ、IL-6、IL-10、CTLA-4和PD-1水平则明显升高,差异有统计学意义(P<0.05)。相关性分析表明,Th1/Th2与AFP和PIVKA负相关性最高,而PD-1与AFP和PIVKA正相关性最高(r>|7|,P<0.05)。结论HCC患者外周血Th1/Th2明显降低,而CTLA-4和PD-1显著升高,且与HCC血清肿瘤标志物AFP和PIVKA具有密切关系,基于上述指标可为HCC的临床诊断和免疫治疗评估提供参考依据。
基金supported by National Natural Science Foundation of China(Grant No.52375272)Zhejiang Provincial Natural Science Foundation of China(Grant No.LR22E050006)China Postdoctoral Science Foundation(Grant Nos.2024M751644,2024M754069).
文摘4D printing technology represents a new generation of additive manufacturing methods that enable three-dimensional(3D)printed structures to change their shapes or properties over time(the fourth dimension)in response to external stimuli such as temperature,magnetic fields,and light.Among the most popular types of 4D-printed structures are thermally responsive bilayer actuators using shape memory polymers,valued for their programmability and convenience.However,achieving precise deformations without collisions is hindered by the nonlinear and time-varying morphing process of these bilayer actuators,which is crucial for creating dynamically controllable shapes on demand in 4D printing.This study presents a rapid and effective design and optimization strategy for 4D printed self-folding structures that can be sequentially and accurately folded.Theoretical analyses were conducted to guide the design of the folding processes.The response surface method(RSM)was used to investigate key parameters affecting the design of 4D-printed bilayer actuators.The results indicate that increasing printing speed enhances internal strain,whereas higher printing temperatures,layer heights,or actuator heights have the opposite effect.The RSM model achieved an R-squared value of 0.983,accurately capturing the coupling effects of these variables on the output responses,thereby enabling controlled timescales for bending motion and sequential folding without collisions.These findings can be applied to enhance the design and acceleration of 4D-printed self-folding structures,ensuring controlled speed of shape transformation.To validate this concept,a self-folding hand-shaped structure with five fingers was designed and fabricated,demonstrating how design and printing parameters can precisely control the timescale of shape changes for each finger based on the design specifications.
