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The fast flight stabilization strategy in flying insects
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作者 Xuefei Cai Hao Liu 《Theoretical & Applied Mechanics Letters》 2025年第5期427-437,共11页
Flying insects demonstrate remarkable control over their body movements and orientation,enabling them to perform rapid maneuvers and withstand external disturbances in just a few wing beats.This fast flight stabilizat... Flying insects demonstrate remarkable control over their body movements and orientation,enabling them to perform rapid maneuvers and withstand external disturbances in just a few wing beats.This fast flight stabilization mechanism has captured the interest of biologists and engineers,driving the exploration of flapping-wing flight control systems and their potential applications in bioinspired flying robots.While many control models have been developed within a rigorous mathematical framework using linear feedback systems,such as proportional(P),integral(I),and derivative(D)-based controllers,the exact mechanisms by which insects achieve the fastest stabilization-despite constraints such as passive aerodynamic damping and feedback delay-remain unclear.In this study,we demonstrate that flying insects employ a novel strategy for fast flight stabilization by minimizing the restoration time under external perturbations.We introduce a versatile PD-based control model that solves the closed-loop dynamics of insect flight and optimizes flight stabilization within a mathematical framework.Our findings reveal that passive aerodynamic damping plays a crucial role in stabilizing flight,acting as derivative feedback without delay,whereas feedback delay hinders stabilization.Additionally,we show that minimizing the restoring time leads to the fastest flight stabilization.Hovering flight analyses of fruit flies,honeybees,hawkmoths,and hummingbirds suggest that restoring time minimization through dynamic oscillatory modes rather than closed-loop time constants is a common strategy among small bioflies for effective maneuvering against disturbances.This strategy,which spans a broad range of Reynolds numbers(on the order of 102 to 104),could offer valuable insights for designing flight controllers in bioinspired flying robots. 展开更多
关键词 flying insects Flight stabilization Proportional-derivative control Oscillatory mode Restoring time
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Flight control of a flying wing aircraft based on circulation control using synthetic jet actuators 被引量:5
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作者 Zhijie ZHAO Xiong DENG +3 位作者 Zhenbing LUO Wenqiang PENG Jianyuan ZHANG Jiefu LIU 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2023年第10期152-164,共13页
To achieve the nice stealth performance and aerodynamic maneuverability of a Flying Wing Aircraft(FWA),a longitudinal aerodynamic control technology based on circulation control using trailing-edge synthetic jet actua... To achieve the nice stealth performance and aerodynamic maneuverability of a Flying Wing Aircraft(FWA),a longitudinal aerodynamic control technology based on circulation control using trailing-edge synthetic jet actuators was proposed without the movement of rudders.Effects on the longitudinal aerodynamic characteristics of a small-sweep FWA were investigated.Then,flight tests were carried out to verify the control abilities,providing a novel technology for the design of a future rudderless FWA.Results show that synthetic jets could narrow the dead zone area,improve the flow velocity near the trailing edge,and then move the trailing-edge separation point and the leading-edge stagnation point downwards,which make the effective Attack of Angle(AOA)increase,thereby enhancing the pressure envelope area.Circulation control based on synthetic jets could improve the lift,drag and nose-down moment.The variations of lift and nosedown moment decrease with the growth of AOA caused by the improved reverse pressure gradient and the weakened circulation control efficiency.Finally,synthetic jet actuators were integrated into the trailing edge of a small-sweep FWA,which could realize the roll and pitch control without deflections of rudders during the cruise stage,and the maximum roll and pitch angular velocity are 12.64(°)/s and 8.51(°)/s,respectively. 展开更多
关键词 Synthetic jets flying wing aircraft Circulation control Control mechanism Flight test
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Three-axis coupled flight control law design forflying wing aircraft using eigenstructure assignment method 被引量:3
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作者 Lixin WANG Ning ZHANG +3 位作者 Ting YUE Hailiang LIU Jianghui ZHU Xiaopeng JIA 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2020年第10期2510-2526,共17页
Due to elimination of horizontal and vertical tails,flying wing aircraft has poor longitudinal and directional dynamic characteristics.In addition,flying wing aircraft uses drag rudders for yaw control,which tends to ... Due to elimination of horizontal and vertical tails,flying wing aircraft has poor longitudinal and directional dynamic characteristics.In addition,flying wing aircraft uses drag rudders for yaw control,which tends to generate strong three-axis control coupling.To overcome these problems,a flight control law design method that couples the longitudinal axis with the lateraldirectional axes is proposed.First,the three-axis coupled control augmentation structure is specified.In the structure,a‘‘soft/hard"cross-connection method is developed for three-axis dynamic decoupling and longitudinal control response decoupling from the drag rudders;maneuvering turn angular rate estimation and subtraction are used in the yaw axis to improve the directional damping.Besides,feedforward control is adopted to improve the maneuverability and control decoupling performance.Then,detailed design methods for feedback and feedforward control parameters are established using eigenstructure assignment and model following technique.Finally,the proposed design method is evaluated and compared with conventional method by numeric simulations.The influences of control derivatives variation of drag rudders on the method are also analyzed.It is demonstrated that the method can effectively improve the dynamic characteristics of flying wing aircraft,especially the directional damping characteristics,and decouple the longitudinal responses from the drag rudders. 展开更多
关键词 Drag rudder Eigenstructure assignment Flight control law flying wing Three-axis coupled
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Virtual flight test of pitch and roll attitude control based on circulation control of tailless flying wing aircraft without rudders 被引量:5
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作者 Liu ZHANG Yong HUANG +4 位作者 Zhenglong ZHU Lihua GAO Fuzheng CHEN Fuzhang WU Meng HE 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2023年第6期52-62,共11页
Circulation Control(CC) realizes rudderless flight control by driving compressed air jet to generate a virtual rudder surface, which significantly improves low detectability. The layout plan of combined control rudder... Circulation Control(CC) realizes rudderless flight control by driving compressed air jet to generate a virtual rudder surface, which significantly improves low detectability. The layout plan of combined control rudder surface is proposed based on the tailless flying wing aircraft. The closed-loop jet actuator system and stepless rudder surface switching control strategy are used to quantitatively study the control characteristics of circulation actuator for pitch and roll attitude through 3-DOF virtual flight test in a wind tunnel with a powered model at wind speed of 40 m/s. The results show that the combined use of circulation actuators can achieve bidirectional continuous and stable control of the aircraft’s pitch and roll attitude, with the maximum pitch rate of 12.3(°)/s and the maximum roll rate of 21.5(°)/s;the response time of attitude angular rate varying with the jet pressure ratio is less than 0.02 s, which can satisfy the control response requirements of aircraft motion stability for the control system;the jet rudder surface has a strong moment control ability, and the pitch moment of the jet elevator with a pressure ratio of 1.28 is the same as that of the mechanical elevator with 28° rudder deflection, which can expand the flight control boundary. 展开更多
关键词 Active flow control Circulation Control(CC) flying wing Wind tunnel test Virtual flight test
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Autonomous Formation Flight Control of Large-Sized Flapping-Wing Flying Robots Based on Leader–Follower Strategy 被引量:1
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作者 Hui Xu Yuanpeng Wang +2 位作者 Erzhen Pan Wenfu Xu Dong Xue 《Journal of Bionic Engineering》 SCIE EI CSCD 2023年第6期2542-2558,共17页
Birds in nature exhibit excellent long-distance flight capabilities through formation flight,which could reduce energy consumption and improve flight efficiency.Inspired by the biological habits of birds,this paper pr... Birds in nature exhibit excellent long-distance flight capabilities through formation flight,which could reduce energy consumption and improve flight efficiency.Inspired by the biological habits of birds,this paper proposes an autonomous formation flight control method for Large-sized Flapping-Wing Flying Robots(LFWFRs),which can enhance their search range and flight efficiency.First,the kinematics model for LFWFRs is established.Then,an autonomous flight controller based on this model is designed,which has multiple flight control modes,including attitude stabilization,course keeping,hovering,and so on.Second,a formation flight control method is proposed based on the leader–follower strategy and periodic characteristics of flapping-wing flight.The up and down fluctuation of the fuselage of each LFWFR during wing flapping is considered in the control algorithm to keep the relative distance,which overcomes the trajectory divergence caused by sensor delay and fuselage fluctuation.Third,typical formation flight modes are realized,including straight formation,circular formation,and switching formation.Finally,the outdoor formation flight experiment is carried out,and the proposed autonomous formation flight control method is verified in real environment. 展开更多
关键词 BIONIC Large-sized flapping-wing flying robot HIT-Phoenix Periodic flight characteristics Formation flight Leader follower strategy
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Reconfigurable Flight Control Design for Combat Flying Wing with Multiple Control Surfaces 被引量:5
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作者 WANG Lei WANG Lixin 《Chinese Journal of Aeronautics》 SCIE EI CSCD 2012年第4期493-499,共7页
With control using redundant multiple control surface arrangement and large-deflection drag rudders,a combat flying wing has a higher probability for control surface failures.Therefore,its flight control system must b... With control using redundant multiple control surface arrangement and large-deflection drag rudders,a combat flying wing has a higher probability for control surface failures.Therefore,its flight control system must be able to reconfigure after such failures.Considering three types of typical control surface failures(lock-in-place(LIP),loss-of-effectiveness(LOE) and float),flight control reconfiguration characteristic and capability of such aircraft types are analyzed.Because of the control surface redundancy,the aircraft using the dynamic inversion flight control law already has a control allocation block.In this paper,its flight control configuration during the above failures is achieved by modifying this block.It is shown that such a reconfigurable flight control design is valid,through numerical simulations of flight attitude control task.Results indicate that,in the circumstances of control surface failures with limited degree and the degradation of the flying quality level,a combat flying wing adopting this flight control reconfiguration approach based on control allocation could guarantee its flight safety and perform some flight combat missions. 展开更多
关键词 flight control reconfiguration control allocation control surface failure flying wing multiple control surfaces drag rudder
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FLI通过增加糖代谢途径促进牛卵母细胞体外成熟
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作者 韩僖彤 张楠 +1 位作者 张宁 张家新 《畜牧兽医学报》 北大核心 2025年第6期2778-2789,共12页
为了明确FGF2、LIF和IGF1(FLI)改善牛卵母细胞体外成熟的作用机制。本研究使用屠宰场收集的卵巢,将从卵巢中抽取的卵丘卵母细胞复合体(COCs)随机分为对照组和FLI组,进行体外成熟,每组30枚COCs,每个试验重复3次。体外成熟24 h后检测第一... 为了明确FGF2、LIF和IGF1(FLI)改善牛卵母细胞体外成熟的作用机制。本研究使用屠宰场收集的卵巢,将从卵巢中抽取的卵丘卵母细胞复合体(COCs)随机分为对照组和FLI组,进行体外成熟,每组30枚COCs,每个试验重复3次。体外成熟24 h后检测第一极体排出率、卵丘扩展情况、线粒体膜电位、皮质颗粒分布、培养基中葡萄糖消耗量和丙酮酸含量、卵母细胞氧化还原态、TZPs数量、活性氧含量、相关基因的表达;并统计后续胚胎发育的卵裂率、囊胚率和囊胚细胞数。结果表明,FLI组卵丘扩展指数显著高于对照组(分别为3.16±0.04和2.43±0.02,P<0.001)。与对照组相比,FLI组卵母细胞的卵丘扩展相关基因表达升高(P<0.05),皮质颗粒向卵周迁移增多(P<0.05),但是卵母细胞第一极体排出率、线粒体膜电位没有显著变化(P>0.05)。与对照组相比,FLI组的培养基中葡萄糖的消耗、丙酮酸的含量显著增加(P<0.05),卵母细胞内NADPH显著增加(P<0.001),并且糖代谢相关基因的表达显著上升(P<0.05)。添加FLI可以显著降低卵母细胞内氧化还原水平、FAD++和ROS水平(P<0.05)。IVM 8 h时,FLI组TZPs数量显著高于对照组(分别为106±6.91和78±8.76,P<0.001)。体外受精后,FLI组卵裂率和囊胚率显著高于对照组(分别为(86.49±0.80)%、(37.44±0.42)%和(74.08±0.91)%、(27.34±1.08)%,P<0.05)。结果显示,FLI通过增加糖代谢提高了卵母细胞体外成熟质量和胚胎发育。 展开更多
关键词 卵母细胞体外成熟 FLI 糖代谢
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From flying dreams to programming pioneer
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作者 孙启禄 《疯狂英语(新读写)》 2025年第7期24-27,76,共5页
More than seventy years before airplanes were invented,a twelve⁃year⁃old girl named Ada Lovelace dreamed of flying.She studied birds and experimented with materials to make wings,even writing a guide called Flyology.B... More than seventy years before airplanes were invented,a twelve⁃year⁃old girl named Ada Lovelace dreamed of flying.She studied birds and experimented with materials to make wings,even writing a guide called Flyology.But her curiosity didnt stop there. 展开更多
关键词 GUIDE flyology ada lovelace programming pioneer flying
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Evaporation of sub-millimeter flying dinitrogen tetroxide droplet under high temperature and pressure:Experimental measurement and theoretical modeling
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作者 Lihan FEI Wu ZHU +3 位作者 Yuyuan ZHANG Peng ZHANG Zuohua HUANG Chenglong TANG 《Chinese Journal of Aeronautics》 2025年第5期80-91,共12页
The evaporation behaviors are crucial for the flame location estimation in liquid rocketengines.This work,for the first time,experimentally reports the sub-millimeter droplet evaporationcharacteristics of the corrosiv... The evaporation behaviors are crucial for the flame location estimation in liquid rocketengines.This work,for the first time,experimentally reports the sub-millimeter droplet evaporationcharacteristics of the corrosive dinitrogen tetroxide(NTO,one prevailing hypergolic oxidizer)athigh ambient pressure up to 4.5 MPa.An in-house corrosion-resistant droplet generator is usedto generate isolated flying droplets of sub-millimeter size,which are then exposed in a gas environ-ment with temperatures between 1010 K and 1210 K and pressures in the range between 2.0 MPaand 4.5 MPa,provided by an optical rapid compression machine.Parallelly,a theoretical modelconsidering both the droplet ambient convection and the NTO dissociation is developed.Resultsindicate that firstly,the present theoretical model that considers the transient droplet-ambient con-vection as well as the temperature and pressure dependent rate of dissociation shows good agree-ment with the experimentally observed droplet lifetime.In addition,the flying droplets velocityregress gradually due to momentum exchange with the ambient,which is more prominent at higherpressure.The evaporation caused droplet size reduction is consistent with the classical D^(2)-law pre-diction,in the present temperature and pressure range.Finally,higher temperature and pressureaccelerate the evaporation and an empirical correlation for the temperature and pressure dependentevaporation rate constant is proposed,which shows good agreement with experiment and simula-tion results. 展开更多
关键词 DROPS EVAPORATION flying isolated droplets Dinitrogen tetroxide High pressure effect
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Experimental research on three-axis control of flying-wing aircraft based on active flow control
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作者 Buxian XU Lihao FENG 《Chinese Journal of Aeronautics》 2025年第8期169-186,共18页
The flying-wing aircraft has excellent aerodynamic efficiency and stealth performance.However,due to the lack of tails,the flying-wing aircraft has a serious attitude control problem.In this paper,the effective flow c... The flying-wing aircraft has excellent aerodynamic efficiency and stealth performance.However,due to the lack of tails,the flying-wing aircraft has a serious attitude control problem.In this paper,the effective flow control strategy of three-axis control is proposed by using continuous jets for a flapless flying-wing aircraft.The wind tunnel test of two kinds of flying-wing models,namely one flow control model and one mechanical control model,is conducted,and the control effect is analyzed and compared.By simultaneous blowing of the circulation control actuators inboard and differential blowing of the circulation control actuators outboard,the pitch and roll controls are achieved,respectively.It also has an effective control effect at very large angles of attack where the conventional control surface fails.A linear relationship is found between the increment of the controlled aerodynamic force/moment coefficient and the momentum coefficient for circulation control actuators.Moreover,to resolve the difficulty in yaw control,a novel wingtip jet is proposed based on the concept of the all-moving tip and compared with apex jet and circulation control jet.It is found that the wingtip jet is the most efficient actuator,followed by the simultaneous-blowing circulation control jet.Therefore,based on the research above,two optimized fluidic control configurations are proposed.One employs circulation control jet and wingtip jet,and the other is completely dependent on circulation control jet.Finally,the flow control mechanism of circulation control is discussed.Circulation control significantly accelerates the flow on the upper surface of the airfoil in attached flow and reduces the flow separation region in separated flow,leading to aerodynamic performance improvement.These results provide an important theoretic basis for the flapless flight control of flying-wing aircraft. 展开更多
关键词 Circulation control Three-axis control flying wing Flapless aircraft Wind tunnel test
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Fantasy Transportation:New Rail-Powered Flying Coach
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作者 Shuangyue Zhao Tianyu Wu +4 位作者 Rongjin Wang Peiyin Wen Zilong Fan Zhongyu Jing Yongming He 《Journal of Electronic Research and Application》 2025年第1期88-93,共6页
To address the challenges of long commuting times,traffic congestion,high energy consumption,and emissions in inter-city travel,a new type of flying coach has been developed.This innovation aims to significantly short... To address the challenges of long commuting times,traffic congestion,high energy consumption,and emissions in inter-city travel,a new type of flying coach has been developed.This innovation aims to significantly shorten inter-city commuting times,enhance travel efficiency,and simultaneously reduce energy consumption and emissions.The flying coach integrates rail power supply technology,an intelligent operating system,and advanced new materials,comprising a catenary power supply guide rod and various sensor components.Based on analysis of traditional aircraft design principles,the research team simulated the design of the rail-powered flying coach using software such as AutoCAD and SolidWorks for three-dimensional modeling.The analysis results indicate that,compared to traditional aircraft and rail trains,the design of the new flying coach reduces its overall weight while maintaining carrying capacity,thereby improving commuting efficiency and environmental performance.This development lays a solid foundation for creating a greener,more efficient,and convenient inter-city transportation network. 展开更多
关键词 Public transportation Rail power flying coach Carbon emission reduction New energy supply methods
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Passively safe configuration design for spacecraft swarm flying with boundary constraints
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作者 Chenglong XU Chengxi ZHANG Jihe WANG 《Chinese Journal of Aeronautics》 2025年第8期399-414,共16页
This paper investigates the configuration design associated with boundary-constrained swarm flying.An analytic swarm configuration is identified to ensure the passive safety between each pair of spacecraft in the radi... This paper investigates the configuration design associated with boundary-constrained swarm flying.An analytic swarm configuration is identified to ensure the passive safety between each pair of spacecraft in the radial-cross-track plane.For the first time,this work derives the explicit configurable spacecraft amount to clarify the configuration's accommodation capacity while considering the maximum inter-spacecraft separation constraint.For larger-scale design problem that involves hundreds of spacecraft,this paper proposes an optimization framework that integrates a Relative Orbit Element(ROE)affine transformation operation and successional convex optimization.The framework establishes a multi-subcluster swarm structure,allowing decoupling the maintenance issues of each subcluster.Compared with previous design methods,it ensures that the computational cost for constraints verification only scales linearly with the swarm size,while also preserving the configuration optimization capacities.Numerical simulations demonstrate that the proposed analytic configuration strictly meets the design constraints.It is also shown that the proposed framework reduces the handled constraint amount by two orders compared with direct optimization,while achieving a remarkable swarm safety enhancement based on the existing analytic configuration. 展开更多
关键词 Collision avoidance Passive safety Relative Eccentricity/Inclination(E/I)vectors Spacecraft swarm flying Swarm configuration design
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软皮汤对硬皮病小鼠模型皮肤组织中p-SMAD3、FLI1因子表达的影响 被引量:10
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作者 齐庆 李永贤 +3 位作者 陈英杰 李东海 朱珂 查旭山 《湖南中医药大学学报》 CAS 2014年第9期19-22,65,共5页
目的前期研究显示软皮汤能改善硬皮病小鼠疾病模型皮肤纤维化程度,本研究进一步探讨软皮汤对纤维化重要通路TGF-β途径的活化因子SMAD3和抑制因子FLI1的影响。方法构建硬皮病小鼠疾病模型,采用Masson三色染色皮肤组织,Western Blot法检... 目的前期研究显示软皮汤能改善硬皮病小鼠疾病模型皮肤纤维化程度,本研究进一步探讨软皮汤对纤维化重要通路TGF-β途径的活化因子SMAD3和抑制因子FLI1的影响。方法构建硬皮病小鼠疾病模型,采用Masson三色染色皮肤组织,Western Blot法检测皮肤CollagenⅠ、Fibronectin、p-SMAD3和FLI1表达水平。结果 (1)Masson染色结果显示,模型对照组动物皮肤中胶原纤维含量明显高于正常对照组(P<0.05);软皮汤可减少软皮汤处理组小鼠皮肤中胶原纤维含量。(2)Western Blot结果显示,模型对照组小鼠皮肤中CollagenⅠ、Fibronectin、p-SMAD3的表达水平高于正常对照组,而FLI1的表达则低于正常对照组(P<P0.05);软皮汤处理组小鼠皮肤中CollagenⅠ、Fibronectin、p-SMAD3的表达水平低于模型对照组,而FLI1的表达则高于模型对照组(P<0.05)。结论软皮汤可改善硬皮病小鼠模型皮肤组织纤维化程度,其机制可能与下调活化因子p-SMAD3、上调抑制因子FLI1有关。 展开更多
关键词 硬皮病 软皮汤 小鼠皮肤组织 p-SMAD3 FLI1
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基于Modelsim FLI接口的FPGA仿真技术 被引量:6
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作者 胡军强 李津生 洪佩琳 《电子技术应用》 北大核心 2002年第7期75-77,共3页
介绍了如何利用modelsim提供的FLI(ForeignLanguageInterface)接口对VHDL设计文件进行协同仿真,给出了协同仿真的意义以及协同仿真的程序结构和系统结构。
关键词 MODELSIM FLI接口 FPGA 仿真软件 协同仿真 VHDL设计 C语言
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尤文肉瘤EWS-FLI1蛋白融合区的二级结构及B细胞表位的预测 被引量:3
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作者 曹凯 黄路 +4 位作者 安洪 舒勇 韩智敏 黄山虎 廖翔 《中国免疫学杂志》 CAS CSCD 北大核心 2008年第1期11-15,共5页
目的:预测尤文肉瘤EWS-FLI1蛋白融合区的二级结构及B细胞表位。方法:采用SOPM/SOPMA法、Chou-Fasman法和Karplus-Schulz法预测EWS-FLI1蛋白的二级结构;综合分析蛋白的柔性结构、亲水性、表面可及性与抗原性,通过预测数据再确定EWS-FLI1... 目的:预测尤文肉瘤EWS-FLI1蛋白融合区的二级结构及B细胞表位。方法:采用SOPM/SOPMA法、Chou-Fasman法和Karplus-Schulz法预测EWS-FLI1蛋白的二级结构;综合分析蛋白的柔性结构、亲水性、表面可及性与抗原性,通过预测数据再确定EWS-FLI1蛋白融合区的抗原表位。结果:EWS-FLI1蛋白的二级结构主要为柔性区域,位于EWS-FLI1蛋白N端5,23-30,32,36-49,62,69-100,118-123,128-132,135,137-170,173-179,183-271,276-286,291-301,317-319,328-331,346-353,396-400,407-408,426-438区段;α螺旋位于N端10-15,34,55,106-107,306-316,340-345,359-362,386-392区段;β折叠位于N端20,22,50-52,65-67,102-107,272,274,289-290,323,325-327,371-375,380-384,422-424,446-447区段;B细胞表位位于N端69-79,99-114,128-152,167-171,194-208,248-265,397-406区段,融合区B细胞表位位于N端248-265区段。结论:应用多参数预测EWS-FLI1蛋白融合区的二级结构与B细胞表位,为蛋白特征及复合表位疫苗的进一步研制奠定了基础。 展开更多
关键词 尤文肉瘤 EWS—FLI1蛋白 二级结构 B细胞表位
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糖肾宝对高糖诱导小鼠肾小球内皮细胞Fli-1、MCP-1及ICAM-1表达的影响 被引量:4
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作者 向少伟 黄柳慧 +5 位作者 陈宇 胡维 黄露 龙韵 贺西南 许雯雯 《中华中医药杂志》 CAS CSCD 北大核心 2021年第10期5854-5858,共5页
目的:探讨中药复方糖肾宝对高糖环境下肾小球内皮细胞迁移功能、Fli-1及炎症因子表达的影响。方法:合成Fli-1-siRNA并转染小鼠肾小球内皮细胞。体外培养小鼠肾小球内皮细胞分为正常组(正常血清)、高糖组(30mmol/L葡萄糖+正常血清)、高糖... 目的:探讨中药复方糖肾宝对高糖环境下肾小球内皮细胞迁移功能、Fli-1及炎症因子表达的影响。方法:合成Fli-1-siRNA并转染小鼠肾小球内皮细胞。体外培养小鼠肾小球内皮细胞分为正常组(正常血清)、高糖组(30mmol/L葡萄糖+正常血清)、高糖+糖肾宝血清组(30mmol/L葡萄糖+糖肾宝血清)、Fli-1-siRNA组(30mmol/L葡萄糖+Fli-1-siRNA+正常血清)、Fli-1-siRNA+糖肾宝组(30mmol/L葡萄糖+Fli-1-siRNA+糖肾宝血清)、空转组(30mmol/L葡萄糖+Control-siRNA+正常血清)。实时定量PCR检测Fli-1、MCP-1、ICAM-1 mRNA表达水平;Western blot法检测Fli-1、MCP-1、ICAM-1蛋白表达水平;Transwell检测小鼠肾小球内皮细胞迁移率。结果:与正常组比较,高糖诱导各组内皮细胞迁移率显著下降(P<0.01);与高糖组比较,高糖+糖肾宝组、Fli-1-siRNA组和Fli-1-siRNA+糖肾宝组内皮细胞迁移率显著升高(P<0.01)。与正常组比较,高糖组、高糖+糖肾宝组及空转组Fli-1、MCP-1、ICAM-1 mRNA及蛋白表达水平均显著升高(P<0.01,P<0.05);与高糖组比较,高糖+糖肾宝组、Fli-1-siRNA组和Fli-1-siRNA+糖肾宝组Fli-1、MCP-1、ICAM-1 mRNA及蛋白表达水平显著下降(P<0.05,P<0.01)。结论:中药复方糖肾宝可能通过抑制高糖培养小鼠肾小球内皮细胞Fli-1的表达,减少炎症因子的产生,改善内皮细胞迁移率。 展开更多
关键词 糖肾宝 糖尿病肾病 肾小球内皮细胞 FLI-1 炎症反应
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汉滩病毒西安分离84FLi的M片段全基因序列测定及分析 被引量:3
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作者 李新红 杨为松 +4 位作者 杭长寿 白雪帆 马本江 黄长形 李光玉 《第四军医大学学报》 北大核心 2002年第8期728-731,共4页
目的 测定我国肾综合征出血热患者流产胎儿肝脏分离病毒 84 FL i株的全 M片段基因序列 ,了解其分子基础 .方法 采用反转录 -聚合酶链反应 (RT- PCR) ,分节段扩增 M基因片段的 c DNA,直接用 PCR产物或克隆入 p MD18- T载体后进行测序 ... 目的 测定我国肾综合征出血热患者流产胎儿肝脏分离病毒 84 FL i株的全 M片段基因序列 ,了解其分子基础 .方法 采用反转录 -聚合酶链反应 (RT- PCR) ,分节段扩增 M基因片段的 c DNA,直接用 PCR产物或克隆入 p MD18- T载体后进行测序 .结果  84 FL i株的全 M基因序列组成为 :M片段基因共由 36 16个核苷酸组成 ,四种核苷酸的比例分别为A 30 .92 % ,C 18.0 3% ,G 2 1.18% ,T 2 9.87% . GC含量为39.2 1% ,AT含量为 6 0 .79% .最大开放读码框为 4 1~ 344 8,共编码 1135个氨基酸 .核苷酸序列同源性分析表明 84 FL i株与 HTN型同源性高于与其他型汉坦病毒的同源性 ,其中与中国株的同源性较高 ,与 HV114株的同源性为 85 .5 % .与HTNV的国际标准毒株 76 - 118的核苷酸同源性分别为84 .0 % ,氨基酸的同源性为 96 .0 % .结论  84 FL i株属于汉滩型病毒 。 展开更多
关键词 汉滩病毒 84FLi株 序列分析 系统发生树 M片段基因序列 测定
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FLI-1在小圆细胞肿瘤鉴别诊断中的应用 被引量:8
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作者 王宁 郑晓刚 +2 位作者 石群立 周晓军 孟奎 《临床与实验病理学杂志》 CAS CSCD 北大核心 2005年第6期646-649,共4页
目的研究FLI-1和CD99在小圆细胞肿瘤中的表达情况,探讨二者在小圆细胞肿瘤鉴别诊断中的应用价值。方法对46例小圆细胞肿瘤进行FLI-1和CD99免疫组化标记,并结合临床与病理组织学进行对比研究。结果FLI-1在Ew ing肉瘤/外周原始神经外胚叶... 目的研究FLI-1和CD99在小圆细胞肿瘤中的表达情况,探讨二者在小圆细胞肿瘤鉴别诊断中的应用价值。方法对46例小圆细胞肿瘤进行FLI-1和CD99免疫组化标记,并结合临床与病理组织学进行对比研究。结果FLI-1在Ew ing肉瘤/外周原始神经外胚叶肿瘤(EW S/PNET)中阳性表达率为90.5%(19/21),在分化差的滑膜肉瘤、横纹肌肉瘤分别为14.3%(1/7)、22.2%(2/9),而在嗅神经母细胞瘤和间叶软骨肉瘤均无表达。CD99在EW S/PNET中阳性表达率为95.2%(20/21),在分化差的滑膜肉瘤、横纹肌肉瘤、嗅神经母细胞瘤和间叶软骨肉瘤分别为71.4%(5/7)、66.7%(6/9)、75.0%(3/4)和60%(3/5)。FLI-1标记在EW S/PNET的敏感性为90.5%,特异性为88%;而CD99在EW S/PNET的敏感性为95.0%,特异性为32%。FLI-1在EW S/PNET中的特异性明显高于CD99(P<0.05)。结论FLI-1在EW S/PNET诊断中的价值优于CD99,并且可用于小圆细胞肿瘤的鉴别诊断。 展开更多
关键词 小圆细胞肿瘤 肉瘤 Ewing 神经外胚瘤 原始 诊断 鉴别 免疫组织化学 FLI-1 CD99
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A Criterion Based on Closed-loop Pilot-aircraft Systems for Predicting Flying Qualities 被引量:2
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作者 谭文倩 A.V.Efremov 屈香菊 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2010年第5期511-517,共7页
During the process of aircraft design, the mathematical model of pilot control behavior characteristics is always used to predict aircraft flying qualities (FQ). This is one of the important methods to avoid pilot-a... During the process of aircraft design, the mathematical model of pilot control behavior characteristics is always used to predict aircraft flying qualities (FQ). This is one of the important methods to avoid pilot-aircraft adverse coupling. In order to study the FQ criterion based on closed-loop pilot-aircraft systems, first, an experimental database is built, which includes 40 aircraft dynamics configurations and the corresponding flight simulation results. Second, the mathematical pilot models with a set of different aircraft configurations are obtained by this experimental database. Then, two FQ criteria, Neal-Smith criterion and Moscow Aviation Institute (MAI) criterion, are analyzed. And the relationship between the FQ level evaluated by actual pilot and the parameters of closed-loop pilot-aircraft systems is studied. Finally, an improved criterion of aircraft FQ is built based on the above two criteria. This new criterion is further used to predict FQ for four new aircraft dynamics configurations, and the prediction results verify its accuracy and practicability. 展开更多
关键词 pilot-aircraft system flying qualities flight simulation pilot model Neal-Smith criterion
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汉滩病毒西安分离株84FLi的L片段核苷酸序列测定以及分析 被引量:3
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作者 李新红 杨为松 +5 位作者 杭长寿 白雪帆 马本江 黄长形 李光玉 张岩 《中国病毒学》 CSCD 2002年第3期221-225,共5页
采用逆转录 聚合酶链式反应 (RT PCR) ,分节段扩增汉滩病毒 84FLi株的L基因片段cDNA ,纯化的PCR产物片段直接用于序列测定或克隆入 pND18 T载体中进行测序。结果表明 ,84FLi株的L片段cDNA由 6 5 33个核苷酸组成 ,四种核苷酸的比例分别... 采用逆转录 聚合酶链式反应 (RT PCR) ,分节段扩增汉滩病毒 84FLi株的L基因片段cDNA ,纯化的PCR产物片段直接用于序列测定或克隆入 pND18 T载体中进行测序。结果表明 ,84FLi株的L片段cDNA由 6 5 33个核苷酸组成 ,四种核苷酸的比例分别为A33.39% ,C16 .4 3% ,G2 0 .74 % ,T2 9.4 4 %。GC含量为 37.17% ,AT含量为6 2 .83%。推导出的最大开放读码框架为从 38到 6 4 93,共编码 2 15 1个氨基酸。序列同源性分析表明 ,84FLi株核苷酸与HTN型国际标准毒株 76 118的同源性为 83.7% ,差异性为 18.8% ;而与中国株A9的同源性高达 97.6 % ,差异性仅为 2 .4 %。与SEO型代表Seoul80 39的同源性为 75 .2 % ,6 6 .1%~ 6 6 .5 %。L片段的氨基酸比较分析表明 ,L片段与HTN型间的同源性为 97.5 %~ 98.0 % ,而与SEO型的同源性为 83.5 %~ 85 .5 %。与PUC、TUL、SN和AND等其它型汉滩病毒的同源性仅为 6 8.6 %~ 6 9.6 %。结果表明 84FLi株属于HTN型 。 展开更多
关键词 汉滩病毒西安分离株84FLi L片段核苷酸序列 序列测定 系统发生树
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