Fluoroacetamide(FAM)has been employed as a rodenticide for an extended duration,leading to a multitude of inci-dents involving human ingestion poisoning.Currently,FAMs have been prohibited by nations globally;however,...Fluoroacetamide(FAM)has been employed as a rodenticide for an extended duration,leading to a multitude of inci-dents involving human ingestion poisoning.Currently,FAMs have been prohibited by nations globally;however,there are still instances of their illegal usage.Conventional instrument methods are characterized by their time-consuming nature and complex operational procedures,rendering them inadequate for meeting urgent diagnostic needs in patients with acute FAM poisoning.Therefore,there is an immediate need to develop a prompt,user-friendly,and precise immunoassay method for the diagnosis of acute poisoning induced by FAM.A lateral flow immunochro-matography assay(LFIA)was developed in this study for the visual detection of FAMs in blood samples,representing the first report of such an approach.The method exhibited a cut-off value of 0.5 mg/mL under the optimized condi-tions,enabling the entire FAM detection process in blood samples to be completed within a mere 8 min without any pretreatment requirements.Notably,the results were easily discernible by visual inspection alone.These results indi-cate that the developed LFIA holds great promise as a convenient and rapid diagnostic tool for FAM poisoning diag-nosis,thereby offering valuable support for subsequent treatment strategies.展开更多
Nuclear magnetic resonance (NMR) is well known for its capacity to identify thestructure of chemical compounds. But, as we know, it has not yet been used in thedetection and identification of fluorine-containing toxic...Nuclear magnetic resonance (NMR) is well known for its capacity to identify thestructure of chemical compounds. But, as we know, it has not yet been used in thedetection and identification of fluorine-containing toxicants in complicated matrixes.Here some efforts were made in this area to apply this powerful technique totoxicological analysis. Sodium fluoroacetate and fluoroacetamide are two fluorine-containing rodenticidesof high toxicity. Their determination in samples related to intoxication is展开更多
基金supported by Beijing Municipal Science and Technology Commission(Z211100007021007)the earmarked fund for CARS36,the Key R&D Program of Ningxia Hui Autonomous Region(2021BBF02036)the program of Beijing Municipal Education Commission(KM202212448002).
文摘Fluoroacetamide(FAM)has been employed as a rodenticide for an extended duration,leading to a multitude of inci-dents involving human ingestion poisoning.Currently,FAMs have been prohibited by nations globally;however,there are still instances of their illegal usage.Conventional instrument methods are characterized by their time-consuming nature and complex operational procedures,rendering them inadequate for meeting urgent diagnostic needs in patients with acute FAM poisoning.Therefore,there is an immediate need to develop a prompt,user-friendly,and precise immunoassay method for the diagnosis of acute poisoning induced by FAM.A lateral flow immunochro-matography assay(LFIA)was developed in this study for the visual detection of FAMs in blood samples,representing the first report of such an approach.The method exhibited a cut-off value of 0.5 mg/mL under the optimized condi-tions,enabling the entire FAM detection process in blood samples to be completed within a mere 8 min without any pretreatment requirements.Notably,the results were easily discernible by visual inspection alone.These results indi-cate that the developed LFIA holds great promise as a convenient and rapid diagnostic tool for FAM poisoning diag-nosis,thereby offering valuable support for subsequent treatment strategies.
文摘Nuclear magnetic resonance (NMR) is well known for its capacity to identify thestructure of chemical compounds. But, as we know, it has not yet been used in thedetection and identification of fluorine-containing toxicants in complicated matrixes.Here some efforts were made in this area to apply this powerful technique totoxicological analysis. Sodium fluoroacetate and fluoroacetamide are two fluorine-containing rodenticidesof high toxicity. Their determination in samples related to intoxication is
