Cytokine monitoring has attracted great attention due to its significance in the diagnosis and treatment of many diseases,such as tumors,microbial infections,and immunological diseases.Enzyme-linked immunosorbent assa...Cytokine monitoring has attracted great attention due to its significance in the diagnosis and treatment of many diseases,such as tumors,microbial infections,and immunological diseases.Enzyme-linked immunosorbent assay(ELISA)is one of the most popular methods in cytokine detection,ascribing to the lavish signal amplification methods in the ELISA platform.In addition to classical enzymes,other signal amplifiers such as fluorescent probes,artificial nano-enzymes,and photothermal reagents have been applied to reduce the detection limit and produce more sensitive ELISA kits.Due to the accumulative effect of heat,photothermal reagents are promising materials in the signal amplification of ELISA.However,the lack of efficient photothermal generation material at an aggregate scale may delay the further development of this area.In this contribution,based on an efficient organic photothermal aggregate material,an enzyme-free photothermally amplified fluorescent immunosorbent assay system consisting of an assay microfluidic chip and detecting platform was developed.The photothermal nanoparticles with highly efficient photothermal conversion by harvesting energy via excited-state intramolecular motions and enlarging molar absorptivity were successfully prepared.The detection concentration at 50 pg/mL of interleukin-2 was achieved,realizing a signal improvement of detection limits by 20-fold compared to that of previously reported photothermal ELISA.The microscopic imaging integrated with plane sweeping technology provided high spatial resolution and precision,indicating the potential of achieving high throughput profiling at the microscale.Moreover,as an alternative excitation source,light-emitting diode not only provided a more affordable and miniaturized detection system but also revealed the great feasibility of intramolecular motion-induced photothermy nanoparticles for biological analyses.展开更多
Objective: To comprehensively understand the infection status of human T-lymphotropic virus (HTLV) among the voluntary blood donors in Yulin City and accurately assess the impact of HTLV on the current situation of bl...Objective: To comprehensively understand the infection status of human T-lymphotropic virus (HTLV) among the voluntary blood donors in Yulin City and accurately assess the impact of HTLV on the current situation of blood safety in Yulin City. Methods: A total of 113,588 blood samples from voluntary blood donors collected in Yulin City from January 2023 to June 2024 were selected. The HTLV-I/II antibody screening was carried out on these samples using the HTLV enzyme-linked immunosorbent assay (ELISA) kit. For the samples with reactive screening results, further confirmation was performed by means of immunoblotting assay and real-time fluorescent quantitative polymerase chain reaction (qPCR). Results: Among the 113,588 blood samples from voluntary blood donors, 20 samples showed positive reactions for HTLV-I/II antibodies, with an initial screening positive reaction rate of 1.76‱. After confirmation, 8 of them were confirmed to be HTLV-I positive, with a positive rate of 0.7‱, and no HTLV-II positive blood donors were detected. Moreover, all the HTLV-I positive blood donors were Han people from Yulin. In addition, by following up the 4 children of a female positive blood donor, it was found that her eldest daughter was confirmed to be HTLV-I positive, while the test results of the other children were negative. Conclusion: At present, there is no HTLV-II infection among the voluntary blood donors in Yulin City. There is a relatively low level of HTLV-I infection mainly among the local people. Continuous monitoring is still needed in the future. Emphasis should be placed on strengthening the publicity, education and testing work for the close relatives of the confirmed positive blood donors and women during pregnancy and childbirth periods, so as to prevent and reduce the HTLV infection caused by family transmission.展开更多
基金Basic and Applied Basic Research Foundation of Guangdong Province,Grant/Award Number:2023A1515010702National Natural Science Foundation of China,Grant/Award Numbers:31870981,82020108016+2 种基金Innovation and Technology Commission,Grant/Award Number:ITC-CNERC14SC01Research Grants Council,University Grants Committee,Grant/Award Numbers:16306620,GRF 16209820STU Scientific Research Initiation Grant,Grant/Award Number:NTF22023。
文摘Cytokine monitoring has attracted great attention due to its significance in the diagnosis and treatment of many diseases,such as tumors,microbial infections,and immunological diseases.Enzyme-linked immunosorbent assay(ELISA)is one of the most popular methods in cytokine detection,ascribing to the lavish signal amplification methods in the ELISA platform.In addition to classical enzymes,other signal amplifiers such as fluorescent probes,artificial nano-enzymes,and photothermal reagents have been applied to reduce the detection limit and produce more sensitive ELISA kits.Due to the accumulative effect of heat,photothermal reagents are promising materials in the signal amplification of ELISA.However,the lack of efficient photothermal generation material at an aggregate scale may delay the further development of this area.In this contribution,based on an efficient organic photothermal aggregate material,an enzyme-free photothermally amplified fluorescent immunosorbent assay system consisting of an assay microfluidic chip and detecting platform was developed.The photothermal nanoparticles with highly efficient photothermal conversion by harvesting energy via excited-state intramolecular motions and enlarging molar absorptivity were successfully prepared.The detection concentration at 50 pg/mL of interleukin-2 was achieved,realizing a signal improvement of detection limits by 20-fold compared to that of previously reported photothermal ELISA.The microscopic imaging integrated with plane sweeping technology provided high spatial resolution and precision,indicating the potential of achieving high throughput profiling at the microscale.Moreover,as an alternative excitation source,light-emitting diode not only provided a more affordable and miniaturized detection system but also revealed the great feasibility of intramolecular motion-induced photothermy nanoparticles for biological analyses.
文摘Objective: To comprehensively understand the infection status of human T-lymphotropic virus (HTLV) among the voluntary blood donors in Yulin City and accurately assess the impact of HTLV on the current situation of blood safety in Yulin City. Methods: A total of 113,588 blood samples from voluntary blood donors collected in Yulin City from January 2023 to June 2024 were selected. The HTLV-I/II antibody screening was carried out on these samples using the HTLV enzyme-linked immunosorbent assay (ELISA) kit. For the samples with reactive screening results, further confirmation was performed by means of immunoblotting assay and real-time fluorescent quantitative polymerase chain reaction (qPCR). Results: Among the 113,588 blood samples from voluntary blood donors, 20 samples showed positive reactions for HTLV-I/II antibodies, with an initial screening positive reaction rate of 1.76‱. After confirmation, 8 of them were confirmed to be HTLV-I positive, with a positive rate of 0.7‱, and no HTLV-II positive blood donors were detected. Moreover, all the HTLV-I positive blood donors were Han people from Yulin. In addition, by following up the 4 children of a female positive blood donor, it was found that her eldest daughter was confirmed to be HTLV-I positive, while the test results of the other children were negative. Conclusion: At present, there is no HTLV-II infection among the voluntary blood donors in Yulin City. There is a relatively low level of HTLV-I infection mainly among the local people. Continuous monitoring is still needed in the future. Emphasis should be placed on strengthening the publicity, education and testing work for the close relatives of the confirmed positive blood donors and women during pregnancy and childbirth periods, so as to prevent and reduce the HTLV infection caused by family transmission.
