A ratiometric fluorescent sensor（Hoe-NI） was developed by connecting a nucleus targeted Hoechst unit to a naphthalimide dye via ＂click chemistry＂. The sensor achieves high specific nucleus labeling with wash-free ...A ratiometric fluorescent sensor（Hoe-NI） was developed by connecting a nucleus targeted Hoechst unit to a naphthalimide dye via ＂click chemistry＂. The sensor achieves high specific nucleus labeling with wash-free staining method in various kinds of living cells. The fluorescence ratio of the two emission bands（450 nm for Hoechst and 505 nm for naphthalimide） is changed sensitively to the variation of DNA concentrations, which provides the quantitative information in the processes of DNA damage induced by hydroxyl radicals and antitumor drug. Therefore, Hoe-NI is a recommendable sensor for the monitoring of nuclear DNA damage that reveals the health status of cells.展开更多
基金supported by the National Natural Science Foundation of China (Nos. 21376038, 21421005 and 21576040)National Basic Research Program of China (No. 2013CB733702)
文摘A ratiometric fluorescent sensor（Hoe-NI） was developed by connecting a nucleus targeted Hoechst unit to a naphthalimide dye via ＂click chemistry＂. The sensor achieves high specific nucleus labeling with wash-free staining method in various kinds of living cells. The fluorescence ratio of the two emission bands（450 nm for Hoechst and 505 nm for naphthalimide） is changed sensitively to the variation of DNA concentrations, which provides the quantitative information in the processes of DNA damage induced by hydroxyl radicals and antitumor drug. Therefore, Hoe-NI is a recommendable sensor for the monitoring of nuclear DNA damage that reveals the health status of cells.
