Staphylococcal food poisoning is a significant foodborne illness caused by staphylococcal enterotoxins(SEs).Immu-noassays have become the primary method for rapidly detecting harmful bacteria and toxins because of the...Staphylococcal food poisoning is a significant foodborne illness caused by staphylococcal enterotoxins(SEs).Immu-noassays have become the primary method for rapidly detecting harmful bacteria and toxins because of their excel-lent sensitivity and specificity.However,these assays have limitations in that they cannot differentiate between types of SEs and do not provide rapid,on-site,quantitative testing.In this study,a time-resolved fluorescence immuno-chromatography assay(TRFICA)was developed specifically for detecting staphylococcal enterotoxin E(SEE),which is commonly found in dairy products.Compared with a double antibody sandwich enzyme-linked immunosorbent assay,which had a detection limit of 0.028 ng/mL,TRFICA demonstrated comparable sensitivity,enabling SEE quan-tification with a detection limit as low as 0.081 ng/mL in infant formula.Validation by spiking infant formula samples confirmed no cross-reactivity with analogs(recoveries ranged from 93.17%to 128.77%).Furthermore,with an 8-min reaction time and interpretation delivered by a portable TRFICA strip reader,our method demonstrates potential for use in mobile and on-site detection.This study describes a rapid,easy,and reliable method for detecting trace lev-els of SEE in infant formula,which could serve as an early screening tool toward preventing food poisoning in infants and children.展开更多
基金supported by Beijing Municipal Science and Technology Commission(Z211100007021007)the Key R&D Program of Ningxia Hui Autonomous Region(2021BBF02036).
文摘Staphylococcal food poisoning is a significant foodborne illness caused by staphylococcal enterotoxins(SEs).Immu-noassays have become the primary method for rapidly detecting harmful bacteria and toxins because of their excel-lent sensitivity and specificity.However,these assays have limitations in that they cannot differentiate between types of SEs and do not provide rapid,on-site,quantitative testing.In this study,a time-resolved fluorescence immuno-chromatography assay(TRFICA)was developed specifically for detecting staphylococcal enterotoxin E(SEE),which is commonly found in dairy products.Compared with a double antibody sandwich enzyme-linked immunosorbent assay,which had a detection limit of 0.028 ng/mL,TRFICA demonstrated comparable sensitivity,enabling SEE quan-tification with a detection limit as low as 0.081 ng/mL in infant formula.Validation by spiking infant formula samples confirmed no cross-reactivity with analogs(recoveries ranged from 93.17%to 128.77%).Furthermore,with an 8-min reaction time and interpretation delivered by a portable TRFICA strip reader,our method demonstrates potential for use in mobile and on-site detection.This study describes a rapid,easy,and reliable method for detecting trace lev-els of SEE in infant formula,which could serve as an early screening tool toward preventing food poisoning in infants and children.
