A fluorescence immunochromatographic strip was developed in this study for natamycin detection in food. The results showed that the best amount of labeled antibody was 10 μg, for every 50 μl of fluorescent microsphe...A fluorescence immunochromatographic strip was developed in this study for natamycin detection in food. The results showed that the best amount of labeled antibody was 10 μg, for every 50 μl of fluorescent microspheres with a 2.5%(w/v) concentration. This labeled antibody was diluted for 10 times, and the diluted solution was dispensed into conjugate pad at the amount of 3 μl/cm. The concentrations of natamycin labeled BSA for test line and goat anti-mouse IgG for control line were 2.0 and 1 mg/ml, respectively, which performed best. With the best conditions, the limit of detection was 1 ng/ml, the linearity ranged from 2 to 100 ng/ml, the recovery was about 80% to 120%, and the CV was below 23%.展开更多
We report the development of a sensitive,fast,and simple method for the detection of prometryn(PRO)in potato and celery using a lateral flow immunochromatographic assay(LFIA).A monoclonal antibody(mAb)against PRO was ...We report the development of a sensitive,fast,and simple method for the detection of prometryn(PRO)in potato and celery using a lateral flow immunochromatographic assay(LFIA).A monoclonal antibody(mAb)against PRO was prepared under optimized conditions,and the 50%inhibition concentration(IC50)for use in ELISA was determined to be 0.6 ng mL−1.Moreover,the cut-off value for use in an LFIA was 50 ng mL−1.To validate the reliability of the developed LFIA strip,real samples were used,and the recovery rate was found to be 99.3-104.3%.The assays were completed within 8 min,demonstrating the suitability of our developed methods for the detection of PRO in potato and celery.展开更多
In order to achieve fast and quantitative detection of fluorescence immunochromatographic chip,a rapid detection system based on smartphone has been developed.In this system,fluorescent signal from quantum dots(QDs)on...In order to achieve fast and quantitative detection of fluorescence immunochromatographic chip,a rapid detection system based on smartphone has been developed.In this system,fluorescent signal from quantum dots(QDs)on lateral flow test strips(LFTSs)can be accurately extracted,and the system also can calculate the concentration of the analyte.The method of extraction and recognition of fluorescence signal intensity can be applied to different fluorescent chip detection systems.Based on the fluorescence tomography chip image,a specific program is used for image acquisition,processing and data handling.The Sobel operator algorithm was used in the software,which improved greatly the ability of distinguishing between the test area and the background boundary information.Extracting the components from the red format of the fluorescent strips,the high-signal intensity and sensitivity were achieved.The simulation results show that the proposed method can be applied to the detection system of fluorescence immunochromatographic chip.The experimental results show that the signal intensity has a good correlation with the concentration of immunoassay,which indicates the detection system can extract the intensity of fluorescence signal of the chip.展开更多
基金Supported by Ningbo Entry-Exit Inspection and Quarantine Bureau(Ningbo Customs)Science and Technology Project(YK07-2017)
文摘A fluorescence immunochromatographic strip was developed in this study for natamycin detection in food. The results showed that the best amount of labeled antibody was 10 μg, for every 50 μl of fluorescent microspheres with a 2.5%(w/v) concentration. This labeled antibody was diluted for 10 times, and the diluted solution was dispensed into conjugate pad at the amount of 3 μl/cm. The concentrations of natamycin labeled BSA for test line and goat anti-mouse IgG for control line were 2.0 and 1 mg/ml, respectively, which performed best. With the best conditions, the limit of detection was 1 ng/ml, the linearity ranged from 2 to 100 ng/ml, the recovery was about 80% to 120%, and the CV was below 23%.
基金supported by the National Natural Science Foundation of China(22236002).
文摘We report the development of a sensitive,fast,and simple method for the detection of prometryn(PRO)in potato and celery using a lateral flow immunochromatographic assay(LFIA).A monoclonal antibody(mAb)against PRO was prepared under optimized conditions,and the 50%inhibition concentration(IC50)for use in ELISA was determined to be 0.6 ng mL−1.Moreover,the cut-off value for use in an LFIA was 50 ng mL−1.To validate the reliability of the developed LFIA strip,real samples were used,and the recovery rate was found to be 99.3-104.3%.The assays were completed within 8 min,demonstrating the suitability of our developed methods for the detection of PRO in potato and celery.
基金support by the National Natural Scientific Foundation of China(Grant No.81571835,61503246,81672247 and 81671737)National Key Basic Research Program(973 Project)(No.2015CB931802)+1 种基金the 863 High-Tech Project of China(No.2014AA020700)Shanghai Science and Technology Fund(No.13NM1401500 and 15DZ2252000).
文摘In order to achieve fast and quantitative detection of fluorescence immunochromatographic chip,a rapid detection system based on smartphone has been developed.In this system,fluorescent signal from quantum dots(QDs)on lateral flow test strips(LFTSs)can be accurately extracted,and the system also can calculate the concentration of the analyte.The method of extraction and recognition of fluorescence signal intensity can be applied to different fluorescent chip detection systems.Based on the fluorescence tomography chip image,a specific program is used for image acquisition,processing and data handling.The Sobel operator algorithm was used in the software,which improved greatly the ability of distinguishing between the test area and the background boundary information.Extracting the components from the red format of the fluorescent strips,the high-signal intensity and sensitivity were achieved.The simulation results show that the proposed method can be applied to the detection system of fluorescence immunochromatographic chip.The experimental results show that the signal intensity has a good correlation with the concentration of immunoassay,which indicates the detection system can extract the intensity of fluorescence signal of the chip.
