Objective To investigate the prevalence and clinical significance of the centromere protein-F-like(CENP-F-like)immunofluorescence staining pattern in a large patient cohort and through literature review.Methods We ret...Objective To investigate the prevalence and clinical significance of the centromere protein-F-like(CENP-F-like)immunofluorescence staining pattern in a large patient cohort and through literature review.Methods We retrospectively analyzed antinuclear antibody(ANA)immunofluorescence assay results from 191274 patients at West China Hospital of Sichuan University between March 2018 and November 2020.Specific immunological markers were tested in sera with CENP-F-like patterns.Additionally,a narrative review of seven relevant studies was performed for comparison.Results In Southwest China,ANA positivity was found in 32.09%of patients,with the CENP-F-like pattern detected in 0.015%of all cases and 0.05%of ANA-positive individuals.The CENP-F-like pattern appeared predominantly at titers≥1∶320,most often in isolation(68.97%),but also mixed with cytoplasmic speckled patterns.Patients with cancers accounted for the highest proportion(31.03%),including solid tumors and hematologic malignancies.Metastasis was observed in patients with solid tumors,while graft-versus-host disease(GVHD)occurred in those with hematologic malignancies post-transplantation.Autoimmune diseases(AIDs)were diagnosed in 20.69%of cases,all showing disease-specific autoantibodies.These findings were broadly consistent with previous reports and suggest a possible association between the CENP-F-like pattern and malignancies.Conclusion The CENP-F-like pattern is rare in ANA tests but may be associated with clinically important conditions,particularly cancers and AIDs.The occurrence of metastasis and GVHD in patients with this pattern highlights its potential clinical relevance,and concurrent autoantibodies may assist in diagnosing AIDs.展开更多
Cyclo[n]Thiophenes(CnTs)are a distinctive class of π-conjugated macrocyclic molecules that have attracted growing attention owing to their structural aesthetics and organic electronic characteristics.However,the deve...Cyclo[n]Thiophenes(CnTs)are a distinctive class of π-conjugated macrocyclic molecules that have attracted growing attention owing to their structural aesthetics and organic electronic characteristics.However,the development of CnTs has been largely impeded by inefficient synthetic route.In this work,we employ a bridge strategy using bipyridine as bridge to link two quaterthiophene units resulting in Φ-shaped bicyclosystem.This strain-retaining approach improves the synthesis efficiency of the macrocycles.Two new macrocyclic molecules,(4T-2hexyl-2Me)_(2)-DPBP and(4T-2hexyl)_(2)-DPBP,were successfully synthesized in total yield 17%and 16%,respectively.Single-crystal structure of(4T-2hexyl-2Me)_(2)-DPBP reveals that the bipyridine bridge is orthogonally strapped by two quaterthiophene units.Notably,both compounds exhibit aggregation-induced emission enhancement(AIEE)behavior-an unprecedented feature among CnT-based macrocycles.Theoretical calculations reveal that this AIE phenomenon originates from the restriction of intramolecular motion(RIM)in the aggregated state,which suppresses the non-radiative decay channels.These results demonstrate a generalized strategy for the synthesis of functionalπ-conjugated macrocyclic molecules based fluorescent materials.展开更多
Over the past ten years,numerous papers have been published on the use of indocyanine green(ICG)fluorescence in liver surgery for hepatocellular carcinoma(HCC).There are many different applications.The first involves ...Over the past ten years,numerous papers have been published on the use of indocyanine green(ICG)fluorescence in liver surgery for hepatocellular carcinoma(HCC).There are many different applications.The first involves targeting superficial tumors in patients with macronodular cirrhosis and an irregular liver surface.In a minimally invasive setting,the lack of tactile feedback on the hepatic surface makes detecting subcapsular HCC with ultrasound alone challenging.ICG fusion images can mimic the tactile feedback of the hand and act as an ultrasound booster.ICG fluorescence can be used to evaluate tumor residues after minimally invasive thermal ablation.ICG fluorescence imaging can also be used to identify the grade of HCC early on and evaluate the microinvasive component.展开更多
Based on 4'-(1H-tetrazol-5-yl)-[1,1'-biphenyl]-2,4,6-tricarboxylic acid(H_(4)bta)ligand,zinc metal-organic framework(Zn-MOF):{[Zn_(2)(bta)(bpy)_(2)(H_(2)O)]·1.5H_(2)O}_(n)(bpy=2,2'-bipyridine)was desi...Based on 4'-(1H-tetrazol-5-yl)-[1,1'-biphenyl]-2,4,6-tricarboxylic acid(H_(4)bta)ligand,zinc metal-organic framework(Zn-MOF):{[Zn_(2)(bta)(bpy)_(2)(H_(2)O)]·1.5H_(2)O}_(n)(bpy=2,2'-bipyridine)was designed and synthesized by hydrothermal method.Its structure was characterized by elemental analysis,IR spectra,X-ray single crystal diffraction,etc.The asymmetric unit of Zn-MOF contains two crystallographically independent Zn^(2+)ions.Through the connection of Zn^(2+)ions via H_(4)bta,a 1D double-layer network structure is formed.Adjacent double-layer networks further form a 2D supramolecular network through hydrogen bonding.Notably,Zn-MOF exhibited excellent fluorescence properties and could efficiently and sensitively detect various water pollutants:4-nitrophenol(4-NP),Cu^(2+),and pyrimethanil(Pth).Additionally,the mechanism of fluorescence sensing was investigated.CCDC:2432230.展开更多
The level of glutathione(GSH)is significantly associated with numerous pathological processes,thus,real-time detection of the GSH level is of significance for early diagnosis of GSH-related diseases.Herein,we develope...The level of glutathione(GSH)is significantly associated with numerous pathological processes,thus,real-time detection of the GSH level is of significance for early diagnosis of GSH-related diseases.Herein,we developed in vivo second near-infrared(NIR-II)window fluorescence(FL)and ratiometric photoacoustic(RPA)dual-modality imaging of GSH using a GSH-activatable probe(LET-14).LET-14 was synthesized based on a rhodamine hybrid xanthene skeleton with a FL shielding 2,4-dinitrobenzene sulfonyl group that can be specifically cleaved by GSH,thus resulting in a markedly bathochromic-shift absorption,a 6.5-fold increase in NIR-II FL intensity(FL_(920))and a 13-fold increase in RPA signal(PA_(880)/PA_(705))in vitro.Intriguingly,LET-14 exhibits good selectivity and sensitivity for NIR-II FL and RPA dual-modality imaging of GSH in 4T1 tumor-bearing mouse model.Our findings develop an in vivo detection tool of GSH,which has great potential in the field of cancer diagnosis.展开更多
Accurate phenological information is essential for measuring ecosystem dynamics and carbon uptake.Southwest China is one of the country's largest terrestrial carbon sink regions and plays a crucial role in carbon ...Accurate phenological information is essential for measuring ecosystem dynamics and carbon uptake.Southwest China is one of the country's largest terrestrial carbon sink regions and plays a crucial role in carbon peaking and neutrality.However,its complex terrain,fragile ecosystem,and variable climate challenge carbon sink stability.Vegetation phenology significantly impacts carbon absorption and release,making accurate phenological data essential for understanding carbon sequestration dynamics.The widespread distribution of evergreen forests and their weak seasonal variation in canopy introduce significant uncertainties in extracting phenology using traditional remote sensing information in this region.These limitations can lead to inaccurate assessments of carbon sink dynamics.Therefore,precise phenology extraction and analysis are vital for improving ecosystem dynamics and the carbon cycle in Southwest China.Firstly,we employed different ways to evaluate the ability of solar-induced chlorophyll fluorescence(SIF)and traditional remote sensing information to extract phenology.Secondly,based on SIF,we analyzed the spatial and temporal changes in the start of the growing season(SOS),the end of the growing season(EOS),and the length of the growing season(LOS)from 2001 to 2020.Finally,we systematically analyzed the response of SOS and EOS to five preseason climatic factors.The results showed that(1)SIF outperformed traditional remote sensing information in extracting phenology.(2)Vegetation phenology exhibited significant spatial heterogeneity.Moreover,SOS,EOS,and LOS showed trends of advancement,delay,and extension both overall and across all vegetation types.(3)Precipitation was the main factor influencing SOS,while surface downward solar radiation and mean temperature were the main factors affecting EOS,and the phenology of different vegetation types showed a great difference in response to preseason climate factors.These findings improve our understanding of vegetation phenology and its dynamics over Southwest China.展开更多
Accurately assessing the carbon sequestration capacity of forests is crucial for mitigating climate change.Traditional methods for estimating Gross Primary Productivity(GPP)of vegetation involve significant uncertaint...Accurately assessing the carbon sequestration capacity of forests is crucial for mitigating climate change.Traditional methods for estimating Gross Primary Productivity(GPP)of vegetation involve significant uncertainties.As a novel remote sensing approach,Solar-Induced chlorophyll Fluorescence(SIF)is directly related to photosynthesis and has demonstrated strong correlations with GPP across various ecosystems,climate zones,and spatial scales.Current GPP estimation methods based on SIF include Light Use Efficiency(LUE)models,the SCOPE process models,and the latest mechanistic light response(MLR)models.Future research should focus on improving the mechanistic understanding of SIF-related processes and promoting the integration of multi-source remote sensing data with SIF-based modeling to enhance the accuracy and universality of GPP estimation.展开更多
A series of blue and blue‑green Ir(Ⅲ)complexes has been investigated theoretically to explore their electronic structures,photophysical properties,efficiency roll‑off effect,and thermal activation delayed fluorescenc...A series of blue and blue‑green Ir(Ⅲ)complexes has been investigated theoretically to explore their electronic structures,photophysical properties,efficiency roll‑off effect,and thermal activation delayed fluorescence(TADF)properties.All calculations were performed using density functional theory(DFT)and time‑dependent density functional theory(TDDFT).Calculations for electronic structures,frontier molecular orbital characteristics(which determine the efficiency roll‑off effect of the complexes),and photophysical properties were conducted using the Gaussian 09 software package.The calculation of spin‑orbit coupling matrix elements<T|HSOC|S>,which determine the TADF properties of the complexes,was performed using the ORCA software package.The calculation results show that the auxiliary ligand tetraphenylimidodiphosphinate(tpip),a strong electron‑withdrawing group,can mitigate the efficiency roll‑off effect of the complex.Furthermore,TADF is observed in one of the designed complexes,(F_(3)Phppy)_(2)Ir(tpip),where F_(3)Phppy=2‑[4‑(2,4,6‑trifluorophenyl)phenyl]pyridine.展开更多
Existing methods for tracing water pollution sources typically integrate three-dimensional excitationemission matrix(3D-EEM)fluorescence spectroscopy with similarity-based matching algorithms.However,these approaches ...Existing methods for tracing water pollution sources typically integrate three-dimensional excitationemission matrix(3D-EEM)fluorescence spectroscopy with similarity-based matching algorithms.However,these approaches exhibit high error rates in borderline cases and necessitate expert manual review,which limits scalability and introduces inconsistencies between algorithmic outputs and expert judgment.To address these limitations,we propose a large vision-language model(VLM)designed as an“expert agent”to automatically refine similarity scores,ensuring alignment with expert decisions and overcoming key application bottlenecks.The model consists of two core components:(1)rule-based similarity calculation module generate initial spectral similarity scores,and(2)pre-trained large vision-language model fine-tuned via supervised learning and reinforcement learning with human feedback(RLHF)to emulate expert assessments.To facilitate training and evaluation,we introduce two expert-annotated datasets,Spec1k and SpecReason,which capture both quantitative corrections and qualitative reasoning patterns,allowing the model to emulate expert decision-making processes.Experimental results demonstrate that our method achieves 81.45%source attribution accuracy,38.24%higher than rule-based and machine learning baselines.Real-world deployment further validates its effectiveness.展开更多
The increasing demand for flexible displays and wearable electronics has driven extensive efforts to develop stretchable organic lightemitting diodes(OLEDs).A critical challenge in this field is the creation of emissi...The increasing demand for flexible displays and wearable electronics has driven extensive efforts to develop stretchable organic lightemitting diodes(OLEDs).A critical challenge in this field is the creation of emissive layers that combine high efficiency with mechanical robustness.Thermally activated delayed fluorescence(TADF)materials have attracted significant attention as third-generation emitters capable of achieving 100%internal quantum efficiency;however,their application in stretchable OLEDs has been limited.In this study,we propose an elastomer doping strategy.Polyurethane(PU)is incorporated into TADF polymers to improve their mechanical flexibility while maintaining a high luminescent efficiency.The resulting composite films exhibited excellent TADF characteristics and remarkable stretchability(75%).OLEDs fabricated from these materials achieved a maximum external quantum efficiency(EQE)of 14.26%and a peak luminance of 73570 cd·m^(-2),with the PUdoped devices showing a significantly suppressed efficiency roll-off.Additionally,a fully stretchable OLED architecture was designed and operated under tensile strain to maintain stable electroluminescent performance.These results demonstrate that elastomer doping is an effective strategy for balancing the mechanical compliance with optoelectronic performance,offering a promising pathway for the development of high-performance stretchable OLEDs for flexible electronics.展开更多
Bay-site carboxyl functionalized perylene diimide derivative 1,7-COOH-PDI-C_(12)(PDI-COOH)was synthesized and distinct enhanced fluorescence was observed through combining with calcium ion(Ca^(2+))in THF/H_(2)O soluti...Bay-site carboxyl functionalized perylene diimide derivative 1,7-COOH-PDI-C_(12)(PDI-COOH)was synthesized and distinct enhanced fluorescence was observed through combining with calcium ion(Ca^(2+))in THF/H_(2)O solution.The assembly and fluorescence behavior of PDI-COOH/Ca^(2+)were studied in detail by changing hydration state with different concentrations.Based on the differences in assembly morphology and stoichiometric ratios of PDICOOH/Ca^(2+),we proposed the fluorescence emission mechanism of PDI-COOH/Ca^(2+)in THF/H_(2)O and THF,respectively.This work reveals a novel strategy of aggregated state fluorescence enhancement and reminds us of the important role of water in molecular fluorescence emission and assembly.展开更多
The presence of aluminum(Al^(3+))and fluoride(F^(−))ions in the environment can be harmful to ecosystems and human health,highlighting the need for accurate and efficient monitoring.In this paper,an innovative approac...The presence of aluminum(Al^(3+))and fluoride(F^(−))ions in the environment can be harmful to ecosystems and human health,highlighting the need for accurate and efficient monitoring.In this paper,an innovative approach is presented that leverages the power of machine learning to enhance the accuracy and efficiency of fluorescence-based detection for sequential quantitative analysis of aluminum(Al^(3+))and fluoride(F^(−))ions in aqueous solutions.The proposed method involves the synthesis of sulfur-functionalized carbon dots(C-dots)as fluorescence probes,with fluorescence enhancement upon interaction with Al^(3+)ions,achieving a detection limit of 4.2 nmol/L.Subsequently,in the presence of F^(−)ions,fluorescence is quenched,with a detection limit of 47.6 nmol/L.The fingerprints of fluorescence images are extracted using a cross-platform computer vision library in Python,followed by data preprocessing.Subsequently,the fingerprint data is subjected to cluster analysis using the K-means model from machine learning,and the average Silhouette Coefficient indicates excellent model performance.Finally,a regression analysis based on the principal component analysis method is employed to achieve more precise quantitative analysis of aluminum and fluoride ions.The results demonstrate that the developed model excels in terms of accuracy and sensitivity.This groundbreaking model not only showcases exceptional performance but also addresses the urgent need for effective environmental monitoring and risk assessment,making it a valuable tool for safeguarding our ecosystems and public health.展开更多
Plants play a crucial role in maintaining ecological balance and biodiversity.However,plant health is easily affected by environmental stresses.Hence,the rapid and precise monitoring of plant health is crucial for glo...Plants play a crucial role in maintaining ecological balance and biodiversity.However,plant health is easily affected by environmental stresses.Hence,the rapid and precise monitoring of plant health is crucial for global food security and ecological balance.Currently,traditional detection strategies for monitoring plant health mainly rely on expensive equipment and complex operational procedures,which limit their widespread application.Fortunately,near-infrared(NIR)fluorescence and surface-enhanced Raman scattering(SERS)techniques have been recently highlighted in plants.NIR fluorescence imaging holds the advantages of being non-invasive,high-resolution and real-time,which is suitable for rapid screening in large-scale scenarios.While SERS enables highly sensitive and specific detection of trace chemical substances within plant tissues.Therefore,the complementarity of NIR fluorescence and SERS modalities can provide more comprehensive and accurate information for plant disease diagnosis and growth status monitoring.This article summarizes these two modalities in plant applications,and discusses the advantages of multimodal NIR fluorescence/SERS for a better understanding of a plant’s response to stress,thereby improving the accuracy and sensitivity of detection.展开更多
Synergistic therapy using multiple modalities is a highly promising therapeutic strategy.Near-infrared-Ⅱ(NIR-Ⅱ)fluorescence imaging,with its deep penetration and high fidelity,has frequently been employed in the lit...Synergistic therapy using multiple modalities is a highly promising therapeutic strategy.Near-infrared-Ⅱ(NIR-Ⅱ)fluorescence imaging,with its deep penetration and high fidelity,has frequently been employed in the literature to guide and assist treatment.Herein,we report the development of a NIR-Ⅱfluorescence imaging guided multi-therapy platform PDI-DS NPs,which integrates a novel activatable phototheranostic agent PDI-DBU,a H_(2)S donor DPS and an amphiphilic polymer DSPE-m PEG2000.In order to maximize redshift of absorption and emission of PDI derivatives,we introduced an electron donating group DBU on PDI to obtain PDI-DBU.PDI-DBU exhibits a distinct absorption band at 700-900 nm and demonstrates excellent NIR-Ⅱfluorescence emission/imaging properties and good photothermal effects under 808 nm laser irradiation.More importantly,under 808 nm laser irradiation,PDI-DBU could be oxidized,and the photodynamic effect of the material could be subsequently activated under 530 nm laser irradiation,achieving the combination of photothermal and activatable photodynamic dual modality treatment.The H_(2)S donor DPS,when triggered by the abundant glutathione(GSH)within the tumor microenvironment(TME),is capable of generating H_(2)S.On one hand,H_(2)S can inhibit tumor growth by disrupting mitochondrial function,on the other hand,it can also repress the expression of heat shock protein 90(HSP90),thereby reversing tumor cell resistance mechanism against photothermal therapy.The utilization of PDI-DS NPs combined with DPS for efficient tumor ablation has been successfully demonstrated both in vitro and in vivo.This synergistic therapeutic platform thus offers a promising strategy in the field of NIR-Ⅱfluorescence imaging guided tumor therapy.展开更多
Cholecystectomy is extensively employed for the treatment of various gallbladder diseases,including symptomatic cholelithiasis,asymptomatic cholelithiasis with a high risk of gallbladder cancer or complications,non-ca...Cholecystectomy is extensively employed for the treatment of various gallbladder diseases,including symptomatic cholelithiasis,asymptomatic cholelithiasis with a high risk of gallbladder cancer or complications,non-calculous cholecystitis,gallbladder polyps larger than 1.0 cm,and porcelain gallbladder,etc.Currently,laparoscopic cholecystectomy(LC)constitutes over 95%of all cholecystectomy procedures,which is the preferred approach for gallbladder surgery[1,2].展开更多
Volatile aromatic aldehydes,including benzaldehyde(BzH),4-fluorobenzaldehyde(4-F-BzH),4-isobutylbenzaldehyde(4-iBu-BzH),3-trifluoromethylbenzaldehyde(3-CF_(3)-BzH),p-methoxybenzaldehyde(4-MeO-BzH),and o-trifluoromethy...Volatile aromatic aldehydes,including benzaldehyde(BzH),4-fluorobenzaldehyde(4-F-BzH),4-isobutylbenzaldehyde(4-iBu-BzH),3-trifluoromethylbenzaldehyde(3-CF_(3)-BzH),p-methoxybenzaldehyde(4-MeO-BzH),and o-trifluoromethylbenzaldehyde(2-CF_(3)-BzH),are crucial raw materials for the synthesis of various pesticides and pharmaceuticals[1].展开更多
Two novel lanthanide complexes,[Sm_(2)(BA)_(6)(4-OH-terpy)_(2)]·2H_(2)O·2EtOH(1)and[Pr_(2)(BA)_(6)(4-OH-terpy)_(2)(H_(2)O)_(2)]·HBA·H_(2)O(2),where HBA=benzoic acid,4-OH-terpy=4-hydroxy-2,2'∶6...Two novel lanthanide complexes,[Sm_(2)(BA)_(6)(4-OH-terpy)_(2)]·2H_(2)O·2EtOH(1)and[Pr_(2)(BA)_(6)(4-OH-terpy)_(2)(H_(2)O)_(2)]·HBA·H_(2)O(2),where HBA=benzoic acid,4-OH-terpy=4-hydroxy-2,2'∶6',2″-terpyridine,were successfully synthesized using ultrasonic dissolution and the conventional solution method with two mixed ligands HBA and 4-OH-terpy.During the synthesis,4-OH-terpy was involved in the reaction as a neutral ligand,while HBA,in its deprotonated form(BA-),coordinated with the lanthanide ions as an acidic ligand.The crystal structures of these two complexes were precisely determined by single-crystal X-ray diffraction.Elemental analysis,infrared and Raman spectroscopy,and powder X-ray diffraction techniques were also employed to further explore the physicochemical properties of the two complexes.The single-crystal X-ray diffraction data indicate that,despite their structural differences,both complexes belong to the triclinic crystal system P1 space group.The central lanthanide ions have the same coordination number but exhibit different coordination environments.To comprehensively evaluate the thermal stability of these two complexes,comprehensive tests including thermogravimetric analysis,differential thermogravimetric analysis,differential scanning calorimetry,Fourier transform infrared spectroscopy,and mass spectrometry were conducted.Meanwhile,an in-depth investigation was conducted into the 3D infrared stacked images and mass spectra of the gases emitted from the complexes.In addition,studies of the fluorescence properties of complex1 showed that it exhibited fluorescence emission matching the Sm^(3+)characteristic transition.展开更多
Using 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran(TCF)as a near-infrared fluorescent chromophore,we designed and synthesized a TCF-based fluorescent probe TCF-NS by introducing 2,4-dinitrophenyl ether ...Using 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran(TCF)as a near-infrared fluorescent chromophore,we designed and synthesized a TCF-based fluorescent probe TCF-NS by introducing 2,4-dinitrophenyl ether as the recognized site for H_(2)S.The probe TCF-NS displayed a rapid-response fluorescent against H_(2)S with high sensitivity and selection but had no significant fluorescence response to other biothiols.Furthermore,TCF-NS was applied to sense H_(2)S in living cells successfully with minimized cytotoxicity and a large Stokes shift.展开更多
We used the natural product chamomile as a carbon source to synthesize praseodymium(Pr) and nitrogen(N) co-doped biomass carbon dots(Pr/N-BCDs) with remarkable luminescence properties by one-step hydrothermal method.C...We used the natural product chamomile as a carbon source to synthesize praseodymium(Pr) and nitrogen(N) co-doped biomass carbon dots(Pr/N-BCDs) with remarkable luminescence properties by one-step hydrothermal method.Compared with single N-doped BCDs(N-BCDs) and Pr-doped BCDs(Pr-BCDs),Pr/N-BCDs not only showed better fluorescence properties and stability but also achieved a significant increase in quantum yield of 12%.More importantly,under certain conditions,Pr/N-BCDs and 2,4-dinitrophenylhydrazide(2,4-DNPH) had significant fluorescence internal filtration effect(IFE) and dynamic quenching effect,and in the concentration range of0.50-20 μmol·L^(-1),the concentration of 2,4-DNPH had a good linear relationship with the fluorescence quenching signal,and the detection limit was as low as 2.1 nmol·L^(-1).展开更多
In this editorial,we comment on the article by Li et al.We specifically focus on the novel use of multicolor near-infrared fluorescence imaging(MCFI)with indocyanine green in laparoscopic cholecystectomy,which is an i...In this editorial,we comment on the article by Li et al.We specifically focus on the novel use of multicolor near-infrared fluorescence imaging(MCFI)with indocyanine green in laparoscopic cholecystectomy,which is an innovative approach for enhancing biliary visualization during laparoscopic cholecystectomy.This study also highlighted the limitations of conventional single-color fluorescence imaging(SCFI),which relies solely on a green fluorescence signal,leading to challenges such as visual fatigue and difficulty in distinguishing biliary structures from background hepatic tissue.Given the complex anatomy of the biliary system and the challenges of visual fatigue encountered with SCFI,MCFI addresses these issues by enabling the differentiation of biliary structures by mapping the fluorescence intensity across a unique blue-to-purple color spectrum,thus improving the clarity of anatomical structures and reducing the visual strain for surgeons.We also focus specifically on the complications and cautious usage of indocyanine green in this context,as well as the advantages and disadvantages of MCFI and SCFI.Overall,MCFI represents a significant advancement in fluorescence-guided surgery,with the potential to become a standard imaging modality for safer and more effective laparoscopic procedures.展开更多
文摘Objective To investigate the prevalence and clinical significance of the centromere protein-F-like(CENP-F-like)immunofluorescence staining pattern in a large patient cohort and through literature review.Methods We retrospectively analyzed antinuclear antibody(ANA)immunofluorescence assay results from 191274 patients at West China Hospital of Sichuan University between March 2018 and November 2020.Specific immunological markers were tested in sera with CENP-F-like patterns.Additionally,a narrative review of seven relevant studies was performed for comparison.Results In Southwest China,ANA positivity was found in 32.09%of patients,with the CENP-F-like pattern detected in 0.015%of all cases and 0.05%of ANA-positive individuals.The CENP-F-like pattern appeared predominantly at titers≥1∶320,most often in isolation(68.97%),but also mixed with cytoplasmic speckled patterns.Patients with cancers accounted for the highest proportion(31.03%),including solid tumors and hematologic malignancies.Metastasis was observed in patients with solid tumors,while graft-versus-host disease(GVHD)occurred in those with hematologic malignancies post-transplantation.Autoimmune diseases(AIDs)were diagnosed in 20.69%of cases,all showing disease-specific autoantibodies.These findings were broadly consistent with previous reports and suggest a possible association between the CENP-F-like pattern and malignancies.Conclusion The CENP-F-like pattern is rare in ANA tests but may be associated with clinically important conditions,particularly cancers and AIDs.The occurrence of metastasis and GVHD in patients with this pattern highlights its potential clinical relevance,and concurrent autoantibodies may assist in diagnosing AIDs.
基金support from the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB0520000)the National Key R&D Program of China(No.2022YFA1203200)+3 种基金National Natural Science Foundation of China(No.52273170)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(No.2021030)Postdoctoral Fellowship Program of CPSF(No.GZC20232735)BMS Junior Fellow of Beijing National Laboratory For molecular Science(No.2023BMS20111).
文摘Cyclo[n]Thiophenes(CnTs)are a distinctive class of π-conjugated macrocyclic molecules that have attracted growing attention owing to their structural aesthetics and organic electronic characteristics.However,the development of CnTs has been largely impeded by inefficient synthetic route.In this work,we employ a bridge strategy using bipyridine as bridge to link two quaterthiophene units resulting in Φ-shaped bicyclosystem.This strain-retaining approach improves the synthesis efficiency of the macrocycles.Two new macrocyclic molecules,(4T-2hexyl-2Me)_(2)-DPBP and(4T-2hexyl)_(2)-DPBP,were successfully synthesized in total yield 17%and 16%,respectively.Single-crystal structure of(4T-2hexyl-2Me)_(2)-DPBP reveals that the bipyridine bridge is orthogonally strapped by two quaterthiophene units.Notably,both compounds exhibit aggregation-induced emission enhancement(AIEE)behavior-an unprecedented feature among CnT-based macrocycles.Theoretical calculations reveal that this AIE phenomenon originates from the restriction of intramolecular motion(RIM)in the aggregated state,which suppresses the non-radiative decay channels.These results demonstrate a generalized strategy for the synthesis of functionalπ-conjugated macrocyclic molecules based fluorescent materials.
文摘Over the past ten years,numerous papers have been published on the use of indocyanine green(ICG)fluorescence in liver surgery for hepatocellular carcinoma(HCC).There are many different applications.The first involves targeting superficial tumors in patients with macronodular cirrhosis and an irregular liver surface.In a minimally invasive setting,the lack of tactile feedback on the hepatic surface makes detecting subcapsular HCC with ultrasound alone challenging.ICG fusion images can mimic the tactile feedback of the hand and act as an ultrasound booster.ICG fluorescence can be used to evaluate tumor residues after minimally invasive thermal ablation.ICG fluorescence imaging can also be used to identify the grade of HCC early on and evaluate the microinvasive component.
文摘Based on 4'-(1H-tetrazol-5-yl)-[1,1'-biphenyl]-2,4,6-tricarboxylic acid(H_(4)bta)ligand,zinc metal-organic framework(Zn-MOF):{[Zn_(2)(bta)(bpy)_(2)(H_(2)O)]·1.5H_(2)O}_(n)(bpy=2,2'-bipyridine)was designed and synthesized by hydrothermal method.Its structure was characterized by elemental analysis,IR spectra,X-ray single crystal diffraction,etc.The asymmetric unit of Zn-MOF contains two crystallographically independent Zn^(2+)ions.Through the connection of Zn^(2+)ions via H_(4)bta,a 1D double-layer network structure is formed.Adjacent double-layer networks further form a 2D supramolecular network through hydrogen bonding.Notably,Zn-MOF exhibited excellent fluorescence properties and could efficiently and sensitively detect various water pollutants:4-nitrophenol(4-NP),Cu^(2+),and pyrimethanil(Pth).Additionally,the mechanism of fluorescence sensing was investigated.CCDC:2432230.
基金supported by the National Natural Science Foundation of China(Nos.82372116,U23A2097)Guangdong Basic and Applied Basic Research Foundation(No.2022A1515010620)+2 种基金Shenzhen Medical Research Fund(Nos.B2302047,A2302047)Shenzhen Science and Technology Program(No.JCYJ20220818095806014)Research Team Cultivation Program of Shenzhen University(No.2023QNT019).
文摘The level of glutathione(GSH)is significantly associated with numerous pathological processes,thus,real-time detection of the GSH level is of significance for early diagnosis of GSH-related diseases.Herein,we developed in vivo second near-infrared(NIR-II)window fluorescence(FL)and ratiometric photoacoustic(RPA)dual-modality imaging of GSH using a GSH-activatable probe(LET-14).LET-14 was synthesized based on a rhodamine hybrid xanthene skeleton with a FL shielding 2,4-dinitrobenzene sulfonyl group that can be specifically cleaved by GSH,thus resulting in a markedly bathochromic-shift absorption,a 6.5-fold increase in NIR-II FL intensity(FL_(920))and a 13-fold increase in RPA signal(PA_(880)/PA_(705))in vitro.Intriguingly,LET-14 exhibits good selectivity and sensitivity for NIR-II FL and RPA dual-modality imaging of GSH in 4T1 tumor-bearing mouse model.Our findings develop an in vivo detection tool of GSH,which has great potential in the field of cancer diagnosis.
基金supported by the National Natural Science Foundation of China[Grant NO.42401465 and 42401464]Yunnan Fundamental Research Projects[Grant NO.202501AT070343,202401AU070169 and 202401CF070161]+1 种基金Natural Science Fund of Kunming University of Science and Technology(KKZ3202421125)Yunnan Provincial Talent Project“High-level Talent Training Support Plan”[YNWR-QNBJ-2020-031]。
文摘Accurate phenological information is essential for measuring ecosystem dynamics and carbon uptake.Southwest China is one of the country's largest terrestrial carbon sink regions and plays a crucial role in carbon peaking and neutrality.However,its complex terrain,fragile ecosystem,and variable climate challenge carbon sink stability.Vegetation phenology significantly impacts carbon absorption and release,making accurate phenological data essential for understanding carbon sequestration dynamics.The widespread distribution of evergreen forests and their weak seasonal variation in canopy introduce significant uncertainties in extracting phenology using traditional remote sensing information in this region.These limitations can lead to inaccurate assessments of carbon sink dynamics.Therefore,precise phenology extraction and analysis are vital for improving ecosystem dynamics and the carbon cycle in Southwest China.Firstly,we employed different ways to evaluate the ability of solar-induced chlorophyll fluorescence(SIF)and traditional remote sensing information to extract phenology.Secondly,based on SIF,we analyzed the spatial and temporal changes in the start of the growing season(SOS),the end of the growing season(EOS),and the length of the growing season(LOS)from 2001 to 2020.Finally,we systematically analyzed the response of SOS and EOS to five preseason climatic factors.The results showed that(1)SIF outperformed traditional remote sensing information in extracting phenology.(2)Vegetation phenology exhibited significant spatial heterogeneity.Moreover,SOS,EOS,and LOS showed trends of advancement,delay,and extension both overall and across all vegetation types.(3)Precipitation was the main factor influencing SOS,while surface downward solar radiation and mean temperature were the main factors affecting EOS,and the phenology of different vegetation types showed a great difference in response to preseason climate factors.These findings improve our understanding of vegetation phenology and its dynamics over Southwest China.
基金sponsored by the National Natural Science Foundation of China(Grant number 42250205,42471510)the Open Found of Technology Innovation Center for Intelligent Monitoring and Spatial Regulation of Land Carbon Sinks,MNR(CUG-SRCS-0002)the Open Fund of Hubei Key Laboratory of Regional Ecological Process and Environmental Evolution(REEC-OF-202405).
文摘Accurately assessing the carbon sequestration capacity of forests is crucial for mitigating climate change.Traditional methods for estimating Gross Primary Productivity(GPP)of vegetation involve significant uncertainties.As a novel remote sensing approach,Solar-Induced chlorophyll Fluorescence(SIF)is directly related to photosynthesis and has demonstrated strong correlations with GPP across various ecosystems,climate zones,and spatial scales.Current GPP estimation methods based on SIF include Light Use Efficiency(LUE)models,the SCOPE process models,and the latest mechanistic light response(MLR)models.Future research should focus on improving the mechanistic understanding of SIF-related processes and promoting the integration of multi-source remote sensing data with SIF-based modeling to enhance the accuracy and universality of GPP estimation.
文摘A series of blue and blue‑green Ir(Ⅲ)complexes has been investigated theoretically to explore their electronic structures,photophysical properties,efficiency roll‑off effect,and thermal activation delayed fluorescence(TADF)properties.All calculations were performed using density functional theory(DFT)and time‑dependent density functional theory(TDDFT).Calculations for electronic structures,frontier molecular orbital characteristics(which determine the efficiency roll‑off effect of the complexes),and photophysical properties were conducted using the Gaussian 09 software package.The calculation of spin‑orbit coupling matrix elements<T|HSOC|S>,which determine the TADF properties of the complexes,was performed using the ORCA software package.The calculation results show that the auxiliary ligand tetraphenylimidodiphosphinate(tpip),a strong electron‑withdrawing group,can mitigate the efficiency roll‑off effect of the complex.Furthermore,TADF is observed in one of the designed complexes,(F_(3)Phppy)_(2)Ir(tpip),where F_(3)Phppy=2‑[4‑(2,4,6‑trifluorophenyl)phenyl]pyridine.
文摘Existing methods for tracing water pollution sources typically integrate three-dimensional excitationemission matrix(3D-EEM)fluorescence spectroscopy with similarity-based matching algorithms.However,these approaches exhibit high error rates in borderline cases and necessitate expert manual review,which limits scalability and introduces inconsistencies between algorithmic outputs and expert judgment.To address these limitations,we propose a large vision-language model(VLM)designed as an“expert agent”to automatically refine similarity scores,ensuring alignment with expert decisions and overcoming key application bottlenecks.The model consists of two core components:(1)rule-based similarity calculation module generate initial spectral similarity scores,and(2)pre-trained large vision-language model fine-tuned via supervised learning and reinforcement learning with human feedback(RLHF)to emulate expert assessments.To facilitate training and evaluation,we introduce two expert-annotated datasets,Spec1k and SpecReason,which capture both quantitative corrections and qualitative reasoning patterns,allowing the model to emulate expert decision-making processes.Experimental results demonstrate that our method achieves 81.45%source attribution accuracy,38.24%higher than rule-based and machine learning baselines.Real-world deployment further validates its effectiveness.
基金supported by the National Natural Science Foundation of China(Nos.T2441002,22525506,U24A20137,and U22A6002)Strategic Priority Research Program of CAS(No.XDB0520101)+1 种基金National Key R&D Program of China(No.2023YFB3609000)CAS Project for Young Scientists in Basic Research(No.YSBR-053)。
文摘The increasing demand for flexible displays and wearable electronics has driven extensive efforts to develop stretchable organic lightemitting diodes(OLEDs).A critical challenge in this field is the creation of emissive layers that combine high efficiency with mechanical robustness.Thermally activated delayed fluorescence(TADF)materials have attracted significant attention as third-generation emitters capable of achieving 100%internal quantum efficiency;however,their application in stretchable OLEDs has been limited.In this study,we propose an elastomer doping strategy.Polyurethane(PU)is incorporated into TADF polymers to improve their mechanical flexibility while maintaining a high luminescent efficiency.The resulting composite films exhibited excellent TADF characteristics and remarkable stretchability(75%).OLEDs fabricated from these materials achieved a maximum external quantum efficiency(EQE)of 14.26%and a peak luminance of 73570 cd·m^(-2),with the PUdoped devices showing a significantly suppressed efficiency roll-off.Additionally,a fully stretchable OLED architecture was designed and operated under tensile strain to maintain stable electroluminescent performance.These results demonstrate that elastomer doping is an effective strategy for balancing the mechanical compliance with optoelectronic performance,offering a promising pathway for the development of high-performance stretchable OLEDs for flexible electronics.
文摘Bay-site carboxyl functionalized perylene diimide derivative 1,7-COOH-PDI-C_(12)(PDI-COOH)was synthesized and distinct enhanced fluorescence was observed through combining with calcium ion(Ca^(2+))in THF/H_(2)O solution.The assembly and fluorescence behavior of PDI-COOH/Ca^(2+)were studied in detail by changing hydration state with different concentrations.Based on the differences in assembly morphology and stoichiometric ratios of PDICOOH/Ca^(2+),we proposed the fluorescence emission mechanism of PDI-COOH/Ca^(2+)in THF/H_(2)O and THF,respectively.This work reveals a novel strategy of aggregated state fluorescence enhancement and reminds us of the important role of water in molecular fluorescence emission and assembly.
基金supported by the National Natural Science Foundation of China(No.U21A20290)Guangdong Basic and Applied Basic Research Foundation(No.2022A1515011656)+2 种基金the Projects of Talents Recruitment of GDUPT(No.2023rcyj1003)the 2022“Sail Plan”Project of Maoming Green Chemical Industry Research Institute(No.MMGCIRI2022YFJH-Y-024)Maoming Science and Technology Project(No.2023382).
文摘The presence of aluminum(Al^(3+))and fluoride(F^(−))ions in the environment can be harmful to ecosystems and human health,highlighting the need for accurate and efficient monitoring.In this paper,an innovative approach is presented that leverages the power of machine learning to enhance the accuracy and efficiency of fluorescence-based detection for sequential quantitative analysis of aluminum(Al^(3+))and fluoride(F^(−))ions in aqueous solutions.The proposed method involves the synthesis of sulfur-functionalized carbon dots(C-dots)as fluorescence probes,with fluorescence enhancement upon interaction with Al^(3+)ions,achieving a detection limit of 4.2 nmol/L.Subsequently,in the presence of F^(−)ions,fluorescence is quenched,with a detection limit of 47.6 nmol/L.The fingerprints of fluorescence images are extracted using a cross-platform computer vision library in Python,followed by data preprocessing.Subsequently,the fingerprint data is subjected to cluster analysis using the K-means model from machine learning,and the average Silhouette Coefficient indicates excellent model performance.Finally,a regression analysis based on the principal component analysis method is employed to achieve more precise quantitative analysis of aluminum and fluoride ions.The results demonstrate that the developed model excels in terms of accuracy and sensitivity.This groundbreaking model not only showcases exceptional performance but also addresses the urgent need for effective environmental monitoring and risk assessment,making it a valuable tool for safeguarding our ecosystems and public health.
基金funded by the National Natural Science Foundation of China(Nos.22374055,22022404,22074050,82172055)the National Natural Science Foundation of Hubei Province(No.22022CFA033)the Fundamental Research Funds for the Central Universities(Nos.CCNU24JCPT001,CCNU24JCPT020)。
文摘Plants play a crucial role in maintaining ecological balance and biodiversity.However,plant health is easily affected by environmental stresses.Hence,the rapid and precise monitoring of plant health is crucial for global food security and ecological balance.Currently,traditional detection strategies for monitoring plant health mainly rely on expensive equipment and complex operational procedures,which limit their widespread application.Fortunately,near-infrared(NIR)fluorescence and surface-enhanced Raman scattering(SERS)techniques have been recently highlighted in plants.NIR fluorescence imaging holds the advantages of being non-invasive,high-resolution and real-time,which is suitable for rapid screening in large-scale scenarios.While SERS enables highly sensitive and specific detection of trace chemical substances within plant tissues.Therefore,the complementarity of NIR fluorescence and SERS modalities can provide more comprehensive and accurate information for plant disease diagnosis and growth status monitoring.This article summarizes these two modalities in plant applications,and discusses the advantages of multimodal NIR fluorescence/SERS for a better understanding of a plant’s response to stress,thereby improving the accuracy and sensitivity of detection.
基金supported by the National Natural Science Foundation of China(No.22274148)the Science and Technology Development Foundation of Jilin Province(Nos.20220204098YY,20230402045GH,20230402018GH,YDZJ202201ZYTS359,YDZJ202201ZYTS351,20240404070ZP,SKL202302030)the Jilin Province Development and Reform Commission’s Innovation Capacity Building Program(No.2023C041-8)。
文摘Synergistic therapy using multiple modalities is a highly promising therapeutic strategy.Near-infrared-Ⅱ(NIR-Ⅱ)fluorescence imaging,with its deep penetration and high fidelity,has frequently been employed in the literature to guide and assist treatment.Herein,we report the development of a NIR-Ⅱfluorescence imaging guided multi-therapy platform PDI-DS NPs,which integrates a novel activatable phototheranostic agent PDI-DBU,a H_(2)S donor DPS and an amphiphilic polymer DSPE-m PEG2000.In order to maximize redshift of absorption and emission of PDI derivatives,we introduced an electron donating group DBU on PDI to obtain PDI-DBU.PDI-DBU exhibits a distinct absorption band at 700-900 nm and demonstrates excellent NIR-Ⅱfluorescence emission/imaging properties and good photothermal effects under 808 nm laser irradiation.More importantly,under 808 nm laser irradiation,PDI-DBU could be oxidized,and the photodynamic effect of the material could be subsequently activated under 530 nm laser irradiation,achieving the combination of photothermal and activatable photodynamic dual modality treatment.The H_(2)S donor DPS,when triggered by the abundant glutathione(GSH)within the tumor microenvironment(TME),is capable of generating H_(2)S.On one hand,H_(2)S can inhibit tumor growth by disrupting mitochondrial function,on the other hand,it can also repress the expression of heat shock protein 90(HSP90),thereby reversing tumor cell resistance mechanism against photothermal therapy.The utilization of PDI-DS NPs combined with DPS for efficient tumor ablation has been successfully demonstrated both in vitro and in vivo.This synergistic therapeutic platform thus offers a promising strategy in the field of NIR-Ⅱfluorescence imaging guided tumor therapy.
文摘Cholecystectomy is extensively employed for the treatment of various gallbladder diseases,including symptomatic cholelithiasis,asymptomatic cholelithiasis with a high risk of gallbladder cancer or complications,non-calculous cholecystitis,gallbladder polyps larger than 1.0 cm,and porcelain gallbladder,etc.Currently,laparoscopic cholecystectomy(LC)constitutes over 95%of all cholecystectomy procedures,which is the preferred approach for gallbladder surgery[1,2].
基金supported by National Natural Science Foundation of China(22361031,22308260).
文摘Volatile aromatic aldehydes,including benzaldehyde(BzH),4-fluorobenzaldehyde(4-F-BzH),4-isobutylbenzaldehyde(4-iBu-BzH),3-trifluoromethylbenzaldehyde(3-CF_(3)-BzH),p-methoxybenzaldehyde(4-MeO-BzH),and o-trifluoromethylbenzaldehyde(2-CF_(3)-BzH),are crucial raw materials for the synthesis of various pesticides and pharmaceuticals[1].
文摘Two novel lanthanide complexes,[Sm_(2)(BA)_(6)(4-OH-terpy)_(2)]·2H_(2)O·2EtOH(1)and[Pr_(2)(BA)_(6)(4-OH-terpy)_(2)(H_(2)O)_(2)]·HBA·H_(2)O(2),where HBA=benzoic acid,4-OH-terpy=4-hydroxy-2,2'∶6',2″-terpyridine,were successfully synthesized using ultrasonic dissolution and the conventional solution method with two mixed ligands HBA and 4-OH-terpy.During the synthesis,4-OH-terpy was involved in the reaction as a neutral ligand,while HBA,in its deprotonated form(BA-),coordinated with the lanthanide ions as an acidic ligand.The crystal structures of these two complexes were precisely determined by single-crystal X-ray diffraction.Elemental analysis,infrared and Raman spectroscopy,and powder X-ray diffraction techniques were also employed to further explore the physicochemical properties of the two complexes.The single-crystal X-ray diffraction data indicate that,despite their structural differences,both complexes belong to the triclinic crystal system P1 space group.The central lanthanide ions have the same coordination number but exhibit different coordination environments.To comprehensively evaluate the thermal stability of these two complexes,comprehensive tests including thermogravimetric analysis,differential thermogravimetric analysis,differential scanning calorimetry,Fourier transform infrared spectroscopy,and mass spectrometry were conducted.Meanwhile,an in-depth investigation was conducted into the 3D infrared stacked images and mass spectra of the gases emitted from the complexes.In addition,studies of the fluorescence properties of complex1 showed that it exhibited fluorescence emission matching the Sm^(3+)characteristic transition.
基金financially supported by the Natural Science Foundation of Jiangsu Province(Grant No.BK20241181)the State Key Laboratory of AnalyticalChemistry for Life Science,School of Chemistry and Chemical Engineering,Nanjing University(Grant No.SKLACLS2419)。
文摘Using 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran(TCF)as a near-infrared fluorescent chromophore,we designed and synthesized a TCF-based fluorescent probe TCF-NS by introducing 2,4-dinitrophenyl ether as the recognized site for H_(2)S.The probe TCF-NS displayed a rapid-response fluorescent against H_(2)S with high sensitivity and selection but had no significant fluorescence response to other biothiols.Furthermore,TCF-NS was applied to sense H_(2)S in living cells successfully with minimized cytotoxicity and a large Stokes shift.
基金supported by the National Natural Science Foundation of China (Grant No.22063010)the Natural Science Foundation of Shaanxi Province (Grant No.2022QFY07-05)Yan'an Science and Technology Plan Project (Grants No.2022SLJBZ-002, 2023-CYL-193)。
文摘We used the natural product chamomile as a carbon source to synthesize praseodymium(Pr) and nitrogen(N) co-doped biomass carbon dots(Pr/N-BCDs) with remarkable luminescence properties by one-step hydrothermal method.Compared with single N-doped BCDs(N-BCDs) and Pr-doped BCDs(Pr-BCDs),Pr/N-BCDs not only showed better fluorescence properties and stability but also achieved a significant increase in quantum yield of 12%.More importantly,under certain conditions,Pr/N-BCDs and 2,4-dinitrophenylhydrazide(2,4-DNPH) had significant fluorescence internal filtration effect(IFE) and dynamic quenching effect,and in the concentration range of0.50-20 μmol·L^(-1),the concentration of 2,4-DNPH had a good linear relationship with the fluorescence quenching signal,and the detection limit was as low as 2.1 nmol·L^(-1).
文摘In this editorial,we comment on the article by Li et al.We specifically focus on the novel use of multicolor near-infrared fluorescence imaging(MCFI)with indocyanine green in laparoscopic cholecystectomy,which is an innovative approach for enhancing biliary visualization during laparoscopic cholecystectomy.This study also highlighted the limitations of conventional single-color fluorescence imaging(SCFI),which relies solely on a green fluorescence signal,leading to challenges such as visual fatigue and difficulty in distinguishing biliary structures from background hepatic tissue.Given the complex anatomy of the biliary system and the challenges of visual fatigue encountered with SCFI,MCFI addresses these issues by enabling the differentiation of biliary structures by mapping the fluorescence intensity across a unique blue-to-purple color spectrum,thus improving the clarity of anatomical structures and reducing the visual strain for surgeons.We also focus specifically on the complications and cautious usage of indocyanine green in this context,as well as the advantages and disadvantages of MCFI and SCFI.Overall,MCFI represents a significant advancement in fluorescence-guided surgery,with the potential to become a standard imaging modality for safer and more effective laparoscopic procedures.
