Rapid diagnosis of rice bacterial diseases is critical for early warning and precise management during their initial phases.The use of rapid nucleic acid detection on paper-based platforms is an innovative technique t...Rapid diagnosis of rice bacterial diseases is critical for early warning and precise management during their initial phases.The use of rapid nucleic acid detection on paper-based platforms is an innovative technique that offers simplicity,portability,and affordability.However,the temperature dependence of the amplification process and variations in paper device technologies hinder on-site detection of pathogens using paper-based platforms.展开更多
Glial fibrillary acidic protein(GFAP)is one of the discriminative biomarkers for diagnosing traumatic brain injury(TBI),and accurate determination of GFAP is clinically significant.In this study,a novel fluorescence i...Glial fibrillary acidic protein(GFAP)is one of the discriminative biomarkers for diagnosing traumatic brain injury(TBI),and accurate determination of GFAP is clinically significant.In this study,a novel fluorescence immunoassay system was designed.We encapsulated carbon dots with a high fluorescence quantum yield(QY=92.5%)inside silicon nanocapsules to serve as fluorescent markers.These markers were then integrated with the streptavidin(SA)-biotin biomagnification system and immunomagnetic separation technology for the sensitive detection of GFAP.Based on the signal cascade amplification effect of the silicon nanocapsules and SA-biotin,the fluorescence signal of the SA-biotin-modified immunofluorescence nanocapsules increased 3.6-fold compared to the carbon dot-based immunoprobe.The fluorescence immunoassay system was constructed for GFAP using SA-biotin-modified immunocapsules as the sensing probe and immunomagnetic nanoparticles as the immunorecognition probe.The fluorescence immunoassay system can specifically and ultra-sensitively quantify GFAP in blood samples,with a detection range of 10 pg/mL–10 ng/mL and detection limits of 3.2 pg/mL(serum)and 3.6 pg/mL(plasma).Moreover,the fluorescence immunoassay system exhibited prominent recoveries of 99.4%–100.4%(phosphate buffered saline),96%–102.6%(serum),and 93.2%–110.2%(plasma),with favorable specificity and excellent stabilization.The novel fluorescence immunoassay system provides a new approach to the clinical analysis of GFAP and may serve as a potential tool for screening and diagnosing TBI.展开更多
Porcine Contagious Pleuropneumonia(PCP)is a respiratory infectious disease of pigs caused by Actinobacillus pleuropneumoniae.The disease has been prevalent in pig farms since it was first identified in 1957(Pattison e...Porcine Contagious Pleuropneumonia(PCP)is a respiratory infectious disease of pigs caused by Actinobacillus pleuropneumoniae.The disease has been prevalent in pig farms since it was first identified in 1957(Pattison et al.1957).展开更多
Over the past ten years,numerous papers have been published on the use of indocyanine green(ICG)fluorescence in liver surgery for hepatocellular carcinoma(HCC).There are many different applications.The first involves ...Over the past ten years,numerous papers have been published on the use of indocyanine green(ICG)fluorescence in liver surgery for hepatocellular carcinoma(HCC).There are many different applications.The first involves targeting superficial tumors in patients with macronodular cirrhosis and an irregular liver surface.In a minimally invasive setting,the lack of tactile feedback on the hepatic surface makes detecting subcapsular HCC with ultrasound alone challenging.ICG fusion images can mimic the tactile feedback of the hand and act as an ultrasound booster.ICG fluorescence can be used to evaluate tumor residues after minimally invasive thermal ablation.ICG fluorescence imaging can also be used to identify the grade of HCC early on and evaluate the microinvasive component.展开更多
BACKGROUND: Molecular cytogenetics of oncogene HER-2 amplification in primary hepatocellular carcinoma (HCC) is still unknown. The aim of this study was to in vestigate the frequency of HER-2 oncogene amplification in...BACKGROUND: Molecular cytogenetics of oncogene HER-2 amplification in primary hepatocellular carcinoma (HCC) is still unknown. The aim of this study was to in vestigate the frequency of HER-2 oncogene amplification in primary HCC and its relations to clinicopathological pa rameters and prognosis. METHODS: Forty-two surgical samples from patients with primary HCC were detected for their HER-2 oncogene am plification. The number of chromosome 17 and their ratio were tested by dual fluorescence in situ hybridization (FISH) technique, and then the correlations between HER-2 amplification, clinicopathological characteristics and prog nosis were analyzed statistically. RESULTS: HER-2 oncogene amplification was detected in 9 (21.4%) of the 42 primary HCCs, including 4 patient with high copy (HC) (9.5%) and 5 patients with low copy (LC) (11.9%). HER-2 amplification was associated signifi cantly with tumor size and postoperative survival time o HCC patients (P<0.05), and the presence of HER-2 gene amplification was correlated with postoperative relapse (P— 0.257), but not related to sex, age, AFP level, HBV infec tion, histopathological grading and clinical staging of HCC patients (P>0.05). The HER-2 oncogene copy was exa mined in 31 (73.8%) of the 42 primary HCCs, consisting of 9 patients with HER-2 amplification (21.4%) and 22 pa tients with aneuploidy (52.4%). No significant relation were observed between the HER-2 oncogene copy, patien sex, tumor size, histopathological grading, clinical stag ing, postoperative relapse and survival time (P >0.05); bu the HER-2 oncogene copy was correlated significantly to age, AFP level and HBV infection (P <0.05). CONCLUSIONS: There are a lower frequency of HER-2 oncogene amplification and a higher frequency of chromo- some 17 aneuploidy in primary HCC. HER-2 oncogene amplification may be involved in the development and pro- gression of large HCC in some patients, and seems to be a valuably independent prognostic factor predicting the re- currence and poor survival in patients with large HCC.展开更多
Femtosecond time-resolved fluorescence non-collinear optical parametric amplification spec- troscopy can extract the curve of spectral gain from its parametric superfluorescence. This unique spectrum correction method...Femtosecond time-resolved fluorescence non-collinear optical parametric amplification spec- troscopy can extract the curve of spectral gain from its parametric superfluorescence. This unique spectrum correction method enables fluorescence non-collinear optical parametric amplification spectroscopy acquiring the genuine transient fluorescence spectrum of the studied system. In this work we employ fluorescence non-collinear optical parametric amplification spectroscopy technique to study the solvation dynamics of DCM dye in ethanol solution, and confirm that genuine solvation correlation function and shift of peak frequency can be derived from transient fluorescence spectra after the spectral gain correction. It demonstrates that fluorescence non-collinear optical parametric amplification spectroscopy can benefit the research fields, which focuses on both fluorescence intensity dynamics and fluorescence spectral shape evolution.展开更多
Objective: To establish a method to improve the detection of disseminated tumor cells in bone marrow and peripheral blood samples of neuroblastoma patients and analysis of cytogenetic aberration. Methods: Immunofluore...Objective: To establish a method to improve the detection of disseminated tumor cells in bone marrow and peripheral blood samples of neuroblastoma patients and analysis of cytogenetic aberration. Methods: Immunofluorescent staining was performed using a cocktail of primary monoclonal neuroblastoma antibodies (14.G2a, 5.1H11). Fluorescence in situ hybridization was applied with fluorescent probes specific for Nmyc genes afterwards. A novel computer assisted scanning system for automatic search, image analysis and repositioning of these positive cells was developed. Fifty-six bone marrow and peripheral blood samples from 7 patients were evaluated by this method. Results: Fluorescence in situ hybridization can be combined with immunofluorescent staining in detecting Nmyc amplification in neuroblastoma patients. Fluorescence in situ hybridization results correlated well with data obtained by conventional cytogenetic procedures. Conclusion: The technique described allows search of tumor cells in the bone marrow as well as detection of Nmyc amplification in interphase nuclei.展开更多
Recently,the simplified spherical harmonics equations(SP)model has at tracted much att entionin modeling the light propagation in small tissue ggeometriesat visible and near-infrared wave-leng ths.In this paper,we rep...Recently,the simplified spherical harmonics equations(SP)model has at tracted much att entionin modeling the light propagation in small tissue ggeometriesat visible and near-infrared wave-leng ths.In this paper,we report an eficient numerical method for fluorescence moleeular tom-ography(FMT)that combines the advantage of SP model and adaptive hp finite elementmethod(hp-FEM).For purposes of comparison,hp-FEM and h-FEM are,respectively applied tothe reconstruction pro cess with diffusion approximation and SPs model.Simulation experiments on a 3D digital mouse atlas and physical experiments on a phantom are designed to evaluate thereconstruction methods in terms of the location and the reconstructed fluorescent yield.Theexperimental results demonstrate that hp-FEM with SPy model,yield more accurate results thanh-FEM with difusion approximation model does.The phantom experiments show the potentialand feasibility of the proposed approach in FMT applications.展开更多
Although the construction of specific functional crystalline materials is still challenging,the multi-component molecular assembly has become a key solution for the design of functional materials.Here,we report a hydr...Although the construction of specific functional crystalline materials is still challenging,the multi-component molecular assembly has become a key solution for the design of functional materials.Here,we report a hydrogen-bonded organic framework(HOF)material FJU-360 constructed from disodium 6‑hydroxy-5-[(4-sulfophenyl)azo]-2-naphthalenesulfonate(SSY)and terephthalimidamide.The charge-assisted hydrogen bonding between amidinium and sulfonate makes FJU-360 produce much stronger fluorescence than SSY,and can be used as a luminescence sensor to rapidly quench aniline through luminescence quenching.FJU-360 is sensitive and highly selective for the detection of aniline,and the detection limit reached 3.2 nmol/L,which is the lowest value reported currently.The mechanism of aniline response was analyzed through the aniline@FJU-360 single crystal structure,and the luminescence mechanism was clarified through density function theory calculations.This work is an important step towards the rational synthesis and assembly of sensing materials.展开更多
Porcine epidemic diarrhea,a highly contagious enteric infectious disease caused by the porcine epidemic diarrhea virus(PEDV)with symptoms of vomit,diarrhea,loss of appetite of suckling pig,has led to serious economic ...Porcine epidemic diarrhea,a highly contagious enteric infectious disease caused by the porcine epidemic diarrhea virus(PEDV)with symptoms of vomit,diarrhea,loss of appetite of suckling pig,has led to serious economic loss to the global swine industry.In this study,a real-time fluorescence reverse transcription loop-mediated isothermal amplification(RT-LAMP)assay was developed to detect PEDV RNA.The real-time fluorescence RT-LAMP assay was performed at62℃for 60 min,using a simple and portable device,the ESE-Quant Tube Scanner.The detection limit of RNA was 2.9×10^(6) copies/μl,10 times as sensitive as RT-PCR,and the detection was specific only to PEDV.Application of this method to clinical samples yielded a positivity rate of 93%,which was higher than that of RT-PCR.This technique saves time and is efficient,and is thus expected to be useful for the diagnosis of PEDV infection in the field.展开更多
A facile and rapid approach for detecting low concentration of iron ion(Fe3+) with improved sensitivity was developed on the basis of plasmon enhanced fluorescence and subsequently amplified fluorescence quenching.Au1...A facile and rapid approach for detecting low concentration of iron ion(Fe3+) with improved sensitivity was developed on the basis of plasmon enhanced fluorescence and subsequently amplified fluorescence quenching.Au1Ag4@Si O2 nanoparticles were synthesized and dispersed into fluorescein isothiocyanate(FITC) solution. The fluorescence of the FITC solution was improved due to plasmon enhanced fluorescence. However, efficient fluorescence quenching of the FITC/Au1Ag4@Si O2 solution was subsequently achieved when Fe3+, with a concentration ranging from17 n M to 3.4 l M, was added into the FITC/Au1Ag4@Si O2 solution, whereas almost no fluorescence quenching was observed for pure FITC solution under the same condition. FITC/Au1Ag4@Si O2 solution shows a better sensitivity for detecting low concentration of Fe3+compared to pure FITC solution. The quantized limit of detection toward Fe3+was improved from 4.6 l M for pure FITC solution to 20 n M for FITC/Au1Ag4@Si O2 solution.展开更多
We demonstrate the output characteristic of broadband parametric amplification of incoherent light pulses in a 355-nm pumped degenerate picosecond optical parametric amplification with either saturated or unsaturated ...We demonstrate the output characteristic of broadband parametric amplification of incoherent light pulses in a 355-nm pumped degenerate picosecond optical parametric amplification with either saturated or unsaturated amplification. The optical parametric amplifier is seeded by the fluorescence generated in a solution of pyridine-1 dye in ethanol. With the saturated amplification, we can obtain high energy incoherent light pulses, whose full width at half maximum bandwidth varies from 16 nm to 53 nm for the different phase matching angles near degeneracy. Moreover, the unsaturated bandwidth of the amplified pulses fits well to the calculated result at degeneracy. Selecting s-polarized fluorescence with a Glan-Taylor prism, the maximum bandwidth of the amplified fluorescence is found to be 59 nm for a purely s-polarized seed. The maximum output energy is 0.67 mJ for the optical parametric amplifier. By using an optical filter and compressor, the generated high energy incoherent light has great potential as the incoherent pump, signal or idler wave of a parametric down-conversion process, so that a wave with a high degree of coherence can be generated from an incoherent pump light.展开更多
Bay-site carboxyl functionalized perylene diimide derivative 1,7-COOH-PDI-C_(12)(PDI-COOH)was synthesized and distinct enhanced fluorescence was observed through combining with calcium ion(Ca^(2+))in THF/H_(2)O soluti...Bay-site carboxyl functionalized perylene diimide derivative 1,7-COOH-PDI-C_(12)(PDI-COOH)was synthesized and distinct enhanced fluorescence was observed through combining with calcium ion(Ca^(2+))in THF/H_(2)O solution.The assembly and fluorescence behavior of PDI-COOH/Ca^(2+)were studied in detail by changing hydration state with different concentrations.Based on the differences in assembly morphology and stoichiometric ratios of PDICOOH/Ca^(2+),we proposed the fluorescence emission mechanism of PDI-COOH/Ca^(2+)in THF/H_(2)O and THF,respectively.This work reveals a novel strategy of aggregated state fluorescence enhancement and reminds us of the important role of water in molecular fluorescence emission and assembly.展开更多
5-Formylcytosine(5fC), as an important epigenetic modification, plays a vital role in diverse biological processes and multiple diseases by regulating gene expression. Owing to the extremely low abundance of 5fC in al...5-Formylcytosine(5fC), as an important epigenetic modification, plays a vital role in diverse biological processes and multiple diseases by regulating gene expression. Owing to the extremely low abundance of 5fC in all mammalian tissues and high structural similarity with other cytosine derivatives, the precise and sensitive detection of 5fC is challenging. Herein, a photo-elutable and template-free isothermal amplification strategy has been proposed for the sensitive detection of 5fC in genomic DNA based on5fC-specific biotinylation, enrichment, photocleavage, and terminal deoxynucleotidyl transferase(Td T)-assisted fluorescence signal amplification, which is termed 5fC-PTIAS. By introducing the highly specific chemolabeling and the one-step photoelution processes, this strategy possesses a minimal nonspecific background as well as a much higher amplification efficiency. With the high signal-to-noise ratio, this strategy can achieve the accurate quantification of 5fC in various biological samples including mouse brain, kidney, and liver, with a limit of detection(LOD) of 0.025‰ in DNA(S/N=3). These results not only confirm the widespread distribution of 5fC but also indicate its significant variation in different tissues and ages. The bisulfite-and mass spectrometry-free strategy is highly sensitive, selective, and easily mastered, holding great promise in detecting other epigenetic modifications with much lower levels.展开更多
Fungal infections are hazardous to human health that has drawn wide attention.In this work,a specific and sensitive method combing the recognition of aptamer to(1,3)-β-D-glucan and tyramide signal amplification techn...Fungal infections are hazardous to human health that has drawn wide attention.In this work,a specific and sensitive method combing the recognition of aptamer to(1,3)-β-D-glucan and tyramide signal amplification technology was proposed for the in situ fluorescence imaging of fungi.Fungi could be distinctly observed by fluorescence microscope rapidly.This method provides morphology and diagnostic information for identifying fungi.The combination of aptamer and tyramide signal amplification technology is a promising tool for the detection of fungi,bacteria and even eukaryotic cell with the virtue of biomarkers.展开更多
The presence of aluminum(Al^(3+))and fluoride(F^(−))ions in the environment can be harmful to ecosystems and human health,highlighting the need for accurate and efficient monitoring.In this paper,an innovative approac...The presence of aluminum(Al^(3+))and fluoride(F^(−))ions in the environment can be harmful to ecosystems and human health,highlighting the need for accurate and efficient monitoring.In this paper,an innovative approach is presented that leverages the power of machine learning to enhance the accuracy and efficiency of fluorescence-based detection for sequential quantitative analysis of aluminum(Al^(3+))and fluoride(F^(−))ions in aqueous solutions.The proposed method involves the synthesis of sulfur-functionalized carbon dots(C-dots)as fluorescence probes,with fluorescence enhancement upon interaction with Al^(3+)ions,achieving a detection limit of 4.2 nmol/L.Subsequently,in the presence of F^(−)ions,fluorescence is quenched,with a detection limit of 47.6 nmol/L.The fingerprints of fluorescence images are extracted using a cross-platform computer vision library in Python,followed by data preprocessing.Subsequently,the fingerprint data is subjected to cluster analysis using the K-means model from machine learning,and the average Silhouette Coefficient indicates excellent model performance.Finally,a regression analysis based on the principal component analysis method is employed to achieve more precise quantitative analysis of aluminum and fluoride ions.The results demonstrate that the developed model excels in terms of accuracy and sensitivity.This groundbreaking model not only showcases exceptional performance but also addresses the urgent need for effective environmental monitoring and risk assessment,making it a valuable tool for safeguarding our ecosystems and public health.展开更多
Plants play a crucial role in maintaining ecological balance and biodiversity.However,plant health is easily affected by environmental stresses.Hence,the rapid and precise monitoring of plant health is crucial for glo...Plants play a crucial role in maintaining ecological balance and biodiversity.However,plant health is easily affected by environmental stresses.Hence,the rapid and precise monitoring of plant health is crucial for global food security and ecological balance.Currently,traditional detection strategies for monitoring plant health mainly rely on expensive equipment and complex operational procedures,which limit their widespread application.Fortunately,near-infrared(NIR)fluorescence and surface-enhanced Raman scattering(SERS)techniques have been recently highlighted in plants.NIR fluorescence imaging holds the advantages of being non-invasive,high-resolution and real-time,which is suitable for rapid screening in large-scale scenarios.While SERS enables highly sensitive and specific detection of trace chemical substances within plant tissues.Therefore,the complementarity of NIR fluorescence and SERS modalities can provide more comprehensive and accurate information for plant disease diagnosis and growth status monitoring.This article summarizes these two modalities in plant applications,and discusses the advantages of multimodal NIR fluorescence/SERS for a better understanding of a plant’s response to stress,thereby improving the accuracy and sensitivity of detection.展开更多
Objective:To explore a method to solve the issue of interference in fluorescence quantitative PCR non-specific amplification for gene detection.Method:A three-step method was used for amplification,and the quantitativ...Objective:To explore a method to solve the issue of interference in fluorescence quantitative PCR non-specific amplification for gene detection.Method:A three-step method was used for amplification,and the quantitative fluorescence signal collection process was set in the extension stage.Results:Three-step amplification has the advantages of wide application range;improved accuracy;and reduced primer design requirements.Conclusion:The interference of non-specific amplification signals was effectively avoided,the melting curve plotting process was omitted,the reaction time was shortened,and the detection accuracy was improved.展开更多
Cholecystectomy is extensively employed for the treatment of various gallbladder diseases,including symptomatic cholelithiasis,asymptomatic cholelithiasis with a high risk of gallbladder cancer or complications,non-ca...Cholecystectomy is extensively employed for the treatment of various gallbladder diseases,including symptomatic cholelithiasis,asymptomatic cholelithiasis with a high risk of gallbladder cancer or complications,non-calculous cholecystitis,gallbladder polyps larger than 1.0 cm,and porcelain gallbladder,etc.Currently,laparoscopic cholecystectomy(LC)constitutes over 95%of all cholecystectomy procedures,which is the preferred approach for gallbladder surgery[1,2].展开更多
基金supported by the National Natural Science Foundation of China(Grant No.32171895)National Key Research and Development Program for Young Scientists,China(Grant No.2022YFD2000200)Jiangsu Province Key Reserch and Development Program Project,China(Grant No.BE2022052-2).
文摘Rapid diagnosis of rice bacterial diseases is critical for early warning and precise management during their initial phases.The use of rapid nucleic acid detection on paper-based platforms is an innovative technique that offers simplicity,portability,and affordability.However,the temperature dependence of the amplification process and variations in paper device technologies hinder on-site detection of pathogens using paper-based platforms.
基金supported by the AMS Funding Project(No.ZZB2023C7010).
文摘Glial fibrillary acidic protein(GFAP)is one of the discriminative biomarkers for diagnosing traumatic brain injury(TBI),and accurate determination of GFAP is clinically significant.In this study,a novel fluorescence immunoassay system was designed.We encapsulated carbon dots with a high fluorescence quantum yield(QY=92.5%)inside silicon nanocapsules to serve as fluorescent markers.These markers were then integrated with the streptavidin(SA)-biotin biomagnification system and immunomagnetic separation technology for the sensitive detection of GFAP.Based on the signal cascade amplification effect of the silicon nanocapsules and SA-biotin,the fluorescence signal of the SA-biotin-modified immunofluorescence nanocapsules increased 3.6-fold compared to the carbon dot-based immunoprobe.The fluorescence immunoassay system was constructed for GFAP using SA-biotin-modified immunocapsules as the sensing probe and immunomagnetic nanoparticles as the immunorecognition probe.The fluorescence immunoassay system can specifically and ultra-sensitively quantify GFAP in blood samples,with a detection range of 10 pg/mL–10 ng/mL and detection limits of 3.2 pg/mL(serum)and 3.6 pg/mL(plasma).Moreover,the fluorescence immunoassay system exhibited prominent recoveries of 99.4%–100.4%(phosphate buffered saline),96%–102.6%(serum),and 93.2%–110.2%(plasma),with favorable specificity and excellent stabilization.The novel fluorescence immunoassay system provides a new approach to the clinical analysis of GFAP and may serve as a potential tool for screening and diagnosing TBI.
基金supported by the University-Industry Col aborative Education Program,China(220904860093831)。
文摘Porcine Contagious Pleuropneumonia(PCP)is a respiratory infectious disease of pigs caused by Actinobacillus pleuropneumoniae.The disease has been prevalent in pig farms since it was first identified in 1957(Pattison et al.1957).
文摘Over the past ten years,numerous papers have been published on the use of indocyanine green(ICG)fluorescence in liver surgery for hepatocellular carcinoma(HCC).There are many different applications.The first involves targeting superficial tumors in patients with macronodular cirrhosis and an irregular liver surface.In a minimally invasive setting,the lack of tactile feedback on the hepatic surface makes detecting subcapsular HCC with ultrasound alone challenging.ICG fusion images can mimic the tactile feedback of the hand and act as an ultrasound booster.ICG fluorescence can be used to evaluate tumor residues after minimally invasive thermal ablation.ICG fluorescence imaging can also be used to identify the grade of HCC early on and evaluate the microinvasive component.
基金This study was supported by grants from the National Outstanding YouthFoundation of China (type B, No. 3982511 ) and the Provincial NaturalScience Foundation of Guangdong, China (No. 980107)
文摘BACKGROUND: Molecular cytogenetics of oncogene HER-2 amplification in primary hepatocellular carcinoma (HCC) is still unknown. The aim of this study was to in vestigate the frequency of HER-2 oncogene amplification in primary HCC and its relations to clinicopathological pa rameters and prognosis. METHODS: Forty-two surgical samples from patients with primary HCC were detected for their HER-2 oncogene am plification. The number of chromosome 17 and their ratio were tested by dual fluorescence in situ hybridization (FISH) technique, and then the correlations between HER-2 amplification, clinicopathological characteristics and prog nosis were analyzed statistically. RESULTS: HER-2 oncogene amplification was detected in 9 (21.4%) of the 42 primary HCCs, including 4 patient with high copy (HC) (9.5%) and 5 patients with low copy (LC) (11.9%). HER-2 amplification was associated signifi cantly with tumor size and postoperative survival time o HCC patients (P<0.05), and the presence of HER-2 gene amplification was correlated with postoperative relapse (P— 0.257), but not related to sex, age, AFP level, HBV infec tion, histopathological grading and clinical staging of HCC patients (P>0.05). The HER-2 oncogene copy was exa mined in 31 (73.8%) of the 42 primary HCCs, consisting of 9 patients with HER-2 amplification (21.4%) and 22 pa tients with aneuploidy (52.4%). No significant relation were observed between the HER-2 oncogene copy, patien sex, tumor size, histopathological grading, clinical stag ing, postoperative relapse and survival time (P >0.05); bu the HER-2 oncogene copy was correlated significantly to age, AFP level and HBV infection (P <0.05). CONCLUSIONS: There are a lower frequency of HER-2 oncogene amplification and a higher frequency of chromo- some 17 aneuploidy in primary HCC. HER-2 oncogene amplification may be involved in the development and pro- gression of large HCC in some patients, and seems to be a valuably independent prognostic factor predicting the re- currence and poor survival in patients with large HCC.
文摘Femtosecond time-resolved fluorescence non-collinear optical parametric amplification spec- troscopy can extract the curve of spectral gain from its parametric superfluorescence. This unique spectrum correction method enables fluorescence non-collinear optical parametric amplification spectroscopy acquiring the genuine transient fluorescence spectrum of the studied system. In this work we employ fluorescence non-collinear optical parametric amplification spectroscopy technique to study the solvation dynamics of DCM dye in ethanol solution, and confirm that genuine solvation correlation function and shift of peak frequency can be derived from transient fluorescence spectra after the spectral gain correction. It demonstrates that fluorescence non-collinear optical parametric amplification spectroscopy can benefit the research fields, which focuses on both fluorescence intensity dynamics and fluorescence spectral shape evolution.
基金This work was supported by a grant fromthe CSC scholarship of the Ministry of Education ofChina(No. 99837010)
文摘Objective: To establish a method to improve the detection of disseminated tumor cells in bone marrow and peripheral blood samples of neuroblastoma patients and analysis of cytogenetic aberration. Methods: Immunofluorescent staining was performed using a cocktail of primary monoclonal neuroblastoma antibodies (14.G2a, 5.1H11). Fluorescence in situ hybridization was applied with fluorescent probes specific for Nmyc genes afterwards. A novel computer assisted scanning system for automatic search, image analysis and repositioning of these positive cells was developed. Fifty-six bone marrow and peripheral blood samples from 7 patients were evaluated by this method. Results: Fluorescence in situ hybridization can be combined with immunofluorescent staining in detecting Nmyc amplification in neuroblastoma patients. Fluorescence in situ hybridization results correlated well with data obtained by conventional cytogenetic procedures. Conclusion: The technique described allows search of tumor cells in the bone marrow as well as detection of Nmyc amplification in interphase nuclei.
基金supported by the National Natural Science Foundation of China(Grant No.61372046)the Research Fund for the Doctoral Program of Higher Education of China(New Teachers)(Grant No.20116101120018)+6 种基金the China Postdoctoral Science Foundation Funded Project(Grant Nos.2011M501467 and 2012T50814)the Natural Science Basic Research Plan in Shaanxi Province of China(Grant No.2011JQ1006)the Fundamental Research Funds for the Central Universities(Grant No.GK201302007)Science and Technology Plan Program,in Shaanxi Province of China(Grant Nos.2012 KJXX-29 and 2013K12-20-12)the Science and Technology Plan Program in Xian of China(Grant No.CXY1348(2))the.GraduateInovation Project of Northwest University(Grant No.YZZ12093)the Seience and Technology Program of Educational Committee,of Shaanxi Province of China(Grant No.12JK0729).
文摘Recently,the simplified spherical harmonics equations(SP)model has at tracted much att entionin modeling the light propagation in small tissue ggeometriesat visible and near-infrared wave-leng ths.In this paper,we report an eficient numerical method for fluorescence moleeular tom-ography(FMT)that combines the advantage of SP model and adaptive hp finite elementmethod(hp-FEM).For purposes of comparison,hp-FEM and h-FEM are,respectively applied tothe reconstruction pro cess with diffusion approximation and SPs model.Simulation experiments on a 3D digital mouse atlas and physical experiments on a phantom are designed to evaluate thereconstruction methods in terms of the location and the reconstructed fluorescent yield.Theexperimental results demonstrate that hp-FEM with SPy model,yield more accurate results thanh-FEM with difusion approximation model does.The phantom experiments show the potentialand feasibility of the proposed approach in FMT applications.
基金supported by the National Natural Science Foundation of China(Nos.21673039,21573042.21805039,21975044,21971038 and 21922810)the Fujian Provincial Department of Education(No.JAT200077).
文摘Although the construction of specific functional crystalline materials is still challenging,the multi-component molecular assembly has become a key solution for the design of functional materials.Here,we report a hydrogen-bonded organic framework(HOF)material FJU-360 constructed from disodium 6‑hydroxy-5-[(4-sulfophenyl)azo]-2-naphthalenesulfonate(SSY)and terephthalimidamide.The charge-assisted hydrogen bonding between amidinium and sulfonate makes FJU-360 produce much stronger fluorescence than SSY,and can be used as a luminescence sensor to rapidly quench aniline through luminescence quenching.FJU-360 is sensitive and highly selective for the detection of aniline,and the detection limit reached 3.2 nmol/L,which is the lowest value reported currently.The mechanism of aniline response was analyzed through the aniline@FJU-360 single crystal structure,and the luminescence mechanism was clarified through density function theory calculations.This work is an important step towards the rational synthesis and assembly of sensing materials.
基金Supported by Science and Technology Research Project of Universities in Hebei Province,China(QN2014220)
文摘Porcine epidemic diarrhea,a highly contagious enteric infectious disease caused by the porcine epidemic diarrhea virus(PEDV)with symptoms of vomit,diarrhea,loss of appetite of suckling pig,has led to serious economic loss to the global swine industry.In this study,a real-time fluorescence reverse transcription loop-mediated isothermal amplification(RT-LAMP)assay was developed to detect PEDV RNA.The real-time fluorescence RT-LAMP assay was performed at62℃for 60 min,using a simple and portable device,the ESE-Quant Tube Scanner.The detection limit of RNA was 2.9×10^(6) copies/μl,10 times as sensitive as RT-PCR,and the detection was specific only to PEDV.Application of this method to clinical samples yielded a positivity rate of 93%,which was higher than that of RT-PCR.This technique saves time and is efficient,and is thus expected to be useful for the diagnosis of PEDV infection in the field.
基金supported by the National Natural Science Foundation of China (51003069)Natural Science Foundation of Jiangsu Higher Education Institutions of China (10KJB430014)A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘A facile and rapid approach for detecting low concentration of iron ion(Fe3+) with improved sensitivity was developed on the basis of plasmon enhanced fluorescence and subsequently amplified fluorescence quenching.Au1Ag4@Si O2 nanoparticles were synthesized and dispersed into fluorescein isothiocyanate(FITC) solution. The fluorescence of the FITC solution was improved due to plasmon enhanced fluorescence. However, efficient fluorescence quenching of the FITC/Au1Ag4@Si O2 solution was subsequently achieved when Fe3+, with a concentration ranging from17 n M to 3.4 l M, was added into the FITC/Au1Ag4@Si O2 solution, whereas almost no fluorescence quenching was observed for pure FITC solution under the same condition. FITC/Au1Ag4@Si O2 solution shows a better sensitivity for detecting low concentration of Fe3+compared to pure FITC solution. The quantized limit of detection toward Fe3+was improved from 4.6 l M for pure FITC solution to 20 n M for FITC/Au1Ag4@Si O2 solution.
基金Project supported by the National Basic Research Program of China (Grant No. 2007CB613205)the National Natural Science Foundation of China (Grant No. 61078005)
文摘We demonstrate the output characteristic of broadband parametric amplification of incoherent light pulses in a 355-nm pumped degenerate picosecond optical parametric amplification with either saturated or unsaturated amplification. The optical parametric amplifier is seeded by the fluorescence generated in a solution of pyridine-1 dye in ethanol. With the saturated amplification, we can obtain high energy incoherent light pulses, whose full width at half maximum bandwidth varies from 16 nm to 53 nm for the different phase matching angles near degeneracy. Moreover, the unsaturated bandwidth of the amplified pulses fits well to the calculated result at degeneracy. Selecting s-polarized fluorescence with a Glan-Taylor prism, the maximum bandwidth of the amplified fluorescence is found to be 59 nm for a purely s-polarized seed. The maximum output energy is 0.67 mJ for the optical parametric amplifier. By using an optical filter and compressor, the generated high energy incoherent light has great potential as the incoherent pump, signal or idler wave of a parametric down-conversion process, so that a wave with a high degree of coherence can be generated from an incoherent pump light.
文摘Bay-site carboxyl functionalized perylene diimide derivative 1,7-COOH-PDI-C_(12)(PDI-COOH)was synthesized and distinct enhanced fluorescence was observed through combining with calcium ion(Ca^(2+))in THF/H_(2)O solution.The assembly and fluorescence behavior of PDI-COOH/Ca^(2+)were studied in detail by changing hydration state with different concentrations.Based on the differences in assembly morphology and stoichiometric ratios of PDICOOH/Ca^(2+),we proposed the fluorescence emission mechanism of PDI-COOH/Ca^(2+)in THF/H_(2)O and THF,respectively.This work reveals a novel strategy of aggregated state fluorescence enhancement and reminds us of the important role of water in molecular fluorescence emission and assembly.
基金supported by the Scientific Technology Project of Shenzhen City(Nos.JCYJ20200109142410170 and JCYJ20210324124003008)the National Natural Science Foundation of China(No.21974153)+2 种基金the Scientific Technology Project of Guangzhou City(No.202103000003)the Guangdong Natural Science Foundation(No.2019A1515010587)the Guangdong Science and Technology Plan Project(No.2020B1212060077)。
文摘5-Formylcytosine(5fC), as an important epigenetic modification, plays a vital role in diverse biological processes and multiple diseases by regulating gene expression. Owing to the extremely low abundance of 5fC in all mammalian tissues and high structural similarity with other cytosine derivatives, the precise and sensitive detection of 5fC is challenging. Herein, a photo-elutable and template-free isothermal amplification strategy has been proposed for the sensitive detection of 5fC in genomic DNA based on5fC-specific biotinylation, enrichment, photocleavage, and terminal deoxynucleotidyl transferase(Td T)-assisted fluorescence signal amplification, which is termed 5fC-PTIAS. By introducing the highly specific chemolabeling and the one-step photoelution processes, this strategy possesses a minimal nonspecific background as well as a much higher amplification efficiency. With the high signal-to-noise ratio, this strategy can achieve the accurate quantification of 5fC in various biological samples including mouse brain, kidney, and liver, with a limit of detection(LOD) of 0.025‰ in DNA(S/N=3). These results not only confirm the widespread distribution of 5fC but also indicate its significant variation in different tissues and ages. The bisulfite-and mass spectrometry-free strategy is highly sensitive, selective, and easily mastered, holding great promise in detecting other epigenetic modifications with much lower levels.
基金supported by the National Natural Science Foundation of China(Nos.22174102,21974101,22174101)the Sino-German rapid response funding call for Covid-19 related research(No.C-0008)。
文摘Fungal infections are hazardous to human health that has drawn wide attention.In this work,a specific and sensitive method combing the recognition of aptamer to(1,3)-β-D-glucan and tyramide signal amplification technology was proposed for the in situ fluorescence imaging of fungi.Fungi could be distinctly observed by fluorescence microscope rapidly.This method provides morphology and diagnostic information for identifying fungi.The combination of aptamer and tyramide signal amplification technology is a promising tool for the detection of fungi,bacteria and even eukaryotic cell with the virtue of biomarkers.
基金supported by the National Natural Science Foundation of China(No.U21A20290)Guangdong Basic and Applied Basic Research Foundation(No.2022A1515011656)+2 种基金the Projects of Talents Recruitment of GDUPT(No.2023rcyj1003)the 2022“Sail Plan”Project of Maoming Green Chemical Industry Research Institute(No.MMGCIRI2022YFJH-Y-024)Maoming Science and Technology Project(No.2023382).
文摘The presence of aluminum(Al^(3+))and fluoride(F^(−))ions in the environment can be harmful to ecosystems and human health,highlighting the need for accurate and efficient monitoring.In this paper,an innovative approach is presented that leverages the power of machine learning to enhance the accuracy and efficiency of fluorescence-based detection for sequential quantitative analysis of aluminum(Al^(3+))and fluoride(F^(−))ions in aqueous solutions.The proposed method involves the synthesis of sulfur-functionalized carbon dots(C-dots)as fluorescence probes,with fluorescence enhancement upon interaction with Al^(3+)ions,achieving a detection limit of 4.2 nmol/L.Subsequently,in the presence of F^(−)ions,fluorescence is quenched,with a detection limit of 47.6 nmol/L.The fingerprints of fluorescence images are extracted using a cross-platform computer vision library in Python,followed by data preprocessing.Subsequently,the fingerprint data is subjected to cluster analysis using the K-means model from machine learning,and the average Silhouette Coefficient indicates excellent model performance.Finally,a regression analysis based on the principal component analysis method is employed to achieve more precise quantitative analysis of aluminum and fluoride ions.The results demonstrate that the developed model excels in terms of accuracy and sensitivity.This groundbreaking model not only showcases exceptional performance but also addresses the urgent need for effective environmental monitoring and risk assessment,making it a valuable tool for safeguarding our ecosystems and public health.
基金funded by the National Natural Science Foundation of China(Nos.22374055,22022404,22074050,82172055)the National Natural Science Foundation of Hubei Province(No.22022CFA033)the Fundamental Research Funds for the Central Universities(Nos.CCNU24JCPT001,CCNU24JCPT020)。
文摘Plants play a crucial role in maintaining ecological balance and biodiversity.However,plant health is easily affected by environmental stresses.Hence,the rapid and precise monitoring of plant health is crucial for global food security and ecological balance.Currently,traditional detection strategies for monitoring plant health mainly rely on expensive equipment and complex operational procedures,which limit their widespread application.Fortunately,near-infrared(NIR)fluorescence and surface-enhanced Raman scattering(SERS)techniques have been recently highlighted in plants.NIR fluorescence imaging holds the advantages of being non-invasive,high-resolution and real-time,which is suitable for rapid screening in large-scale scenarios.While SERS enables highly sensitive and specific detection of trace chemical substances within plant tissues.Therefore,the complementarity of NIR fluorescence and SERS modalities can provide more comprehensive and accurate information for plant disease diagnosis and growth status monitoring.This article summarizes these two modalities in plant applications,and discusses the advantages of multimodal NIR fluorescence/SERS for a better understanding of a plant’s response to stress,thereby improving the accuracy and sensitivity of detection.
文摘Objective:To explore a method to solve the issue of interference in fluorescence quantitative PCR non-specific amplification for gene detection.Method:A three-step method was used for amplification,and the quantitative fluorescence signal collection process was set in the extension stage.Results:Three-step amplification has the advantages of wide application range;improved accuracy;and reduced primer design requirements.Conclusion:The interference of non-specific amplification signals was effectively avoided,the melting curve plotting process was omitted,the reaction time was shortened,and the detection accuracy was improved.
文摘Cholecystectomy is extensively employed for the treatment of various gallbladder diseases,including symptomatic cholelithiasis,asymptomatic cholelithiasis with a high risk of gallbladder cancer or complications,non-calculous cholecystitis,gallbladder polyps larger than 1.0 cm,and porcelain gallbladder,etc.Currently,laparoscopic cholecystectomy(LC)constitutes over 95%of all cholecystectomy procedures,which is the preferred approach for gallbladder surgery[1,2].
