The level of glutathione(GSH)is significantly associated with numerous pathological processes,thus,real-time detection of the GSH level is of significance for early diagnosis of GSH-related diseases.Herein,we develope...The level of glutathione(GSH)is significantly associated with numerous pathological processes,thus,real-time detection of the GSH level is of significance for early diagnosis of GSH-related diseases.Herein,we developed in vivo second near-infrared(NIR-II)window fluorescence(FL)and ratiometric photoacoustic(RPA)dual-modality imaging of GSH using a GSH-activatable probe(LET-14).LET-14 was synthesized based on a rhodamine hybrid xanthene skeleton with a FL shielding 2,4-dinitrobenzene sulfonyl group that can be specifically cleaved by GSH,thus resulting in a markedly bathochromic-shift absorption,a 6.5-fold increase in NIR-II FL intensity(FL920)and a 13-fold increase in RPA signal(PA880/PA705)in vitro.Intriguingly,LET-14 exhibits good selectivity and sensitivity for NIR-II FL and RPA dual-modality imaging of GSH in 4T1 tumor-bearing mouse model.Our findings develop an in vivo detection tool of GSH,which has great potential in the field of cancer diagnosis.展开更多
Sulfur dioxide(SO_(2)) and its derivatives have been recognized as harmful environmental pollutants.However,they are often produced during the processing of traditional Chinese medicines,potentially compromising the q...Sulfur dioxide(SO_(2)) and its derivatives have been recognized as harmful environmental pollutants.However,they are often produced during the processing of traditional Chinese medicines,potentially compromising the quality of these medicinal materials and contributing to various health issues.Due to a lack of effective monitoring and imaging tools,the physiological effects of excessive SO_(2) residues in traditional Chinese medicine remain unclear.Therefore,developing a rapid and effective tool for detecting SO_(2) is crucial for understanding its metabolic pathways and effects in vivo.In this study,we developed a near infrared(NIR) and ratiometric fluorescent probe,NIR-RS,which exhibits high sensitivity,selectivity,and rapid response for SO_(2) detection.Notably,NIR-RS accurately quantifies SO_(2) contents in Pinelliae rhizoma(P.rhizoma) samples,with recovery rates from 98.46 % to 102.40 %,and relative standard deviations(RSDs)< 5.0 %.For bioimaging applications,NIR-RS has low cytotoxicity and good mitochondrial-targeting ability,making it suitable for imaging exogenous and endogenous SO_(2) in mitochondria.Additionally,NIR-RS was successfully applied to image SO_(2) content of P.rhizoma samples within cells,revealing that high SO_(2) residue elevated mitochondria adenosine triphosphate(ATP) content,these findings reveal that P.rhizoma with excessive SO_(2) can affect the organism's growth mechanisms through alterations in ATP pathways.In vivo,SO_(2) was found to predominantly accumulate in the liver following gavage with P.rhizoma solution,with accumulation levels increasing in proportion to SO_(2) residue concentration.High SO_(2) concentrations in P.rhizoma can cause pulmonary fibrosis and gastric mucosal damage.This work provides a valuable tool for regulating SO_(2) content in P.rhizoma and may help researcher better understand the metabolism of SO_(2) derivatives and explore their physiological roles in biological systems.展开更多
Using 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran(TCF)as a near-infrared fluorescent chromophore,we designed and synthesized a TCF-based fluorescent probe TCF-NS by introducing 2,4-dinitrophenyl ether ...Using 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran(TCF)as a near-infrared fluorescent chromophore,we designed and synthesized a TCF-based fluorescent probe TCF-NS by introducing 2,4-dinitrophenyl ether as the recognized site for H_(2)S.The probe TCF-NS displayed a rapid-response fluorescent against H_(2)S with high sensitivity and selection but had no significant fluorescence response to other biothiols.Furthermore,TCF-NS was applied to sense H_(2)S in living cells successfully with minimized cytotoxicity and a large Stokes shift.展开更多
In this editorial,we comment on the article by Li et al.We specifically focus on the novel use of multicolor near-infrared fluorescence imaging(MCFI)with indocyanine green in laparoscopic cholecystectomy,which is an i...In this editorial,we comment on the article by Li et al.We specifically focus on the novel use of multicolor near-infrared fluorescence imaging(MCFI)with indocyanine green in laparoscopic cholecystectomy,which is an innovative approach for enhancing biliary visualization during laparoscopic cholecystectomy.This study also highlighted the limitations of conventional single-color fluorescence imaging(SCFI),which relies solely on a green fluorescence signal,leading to challenges such as visual fatigue and difficulty in distinguishing biliary structures from background hepatic tissue.Given the complex anatomy of the biliary system and the challenges of visual fatigue encountered with SCFI,MCFI addresses these issues by enabling the differentiation of biliary structures by mapping the fluorescence intensity across a unique blue-to-purple color spectrum,thus improving the clarity of anatomical structures and reducing the visual strain for surgeons.We also focus specifically on the complications and cautious usage of indocyanine green in this context,as well as the advantages and disadvantages of MCFI and SCFI.Overall,MCFI represents a significant advancement in fluorescence-guided surgery,with the potential to become a standard imaging modality for safer and more effective laparoscopic procedures.展开更多
Triple-negative breast cancer (TNBC) is an aggressive and often fatal disease, especially since the brain metastasis of TNBC has been a particularly severe manifestation. However, brain metastasis in TNBC at early sta...Triple-negative breast cancer (TNBC) is an aggressive and often fatal disease, especially since the brain metastasis of TNBC has been a particularly severe manifestation. However, brain metastasis in TNBC at early stages often lacks noticeable symptoms, making it challenging to detect. Near-infrared II (NIR-II) fluorescence microscopic imaging obtains long wavelength, which enables reduced scattering, high spatial resolution and minimal autofluorescence, it is also a favorable imaging method for tumor diagnosis. PbS@CdS quantum dots (QDs) are one of the popular NIR-II fluorescence nanoprobes for well brightness. In this study, NIR-II emissive PbS@CdS QDs were utilized and further encapsulated with thiol-terminated poly(ethylene oxide) (SH-PEG, MW = 5000) to form PbS@CdS@PEG QDs nanoparticles (NPs). The obtained PbS@CdS@PEG QDs NPs were then characterized and further studied in detail. The PbS@CdS@PEG QDs NPs had large absorption spectra, exhibited strong NIR-II fluorescence emission at approximately 1300nm, and possessed good NIR-II fluorescence properties. Then, the mice model of early-stage brain metastases of TNBC was established, and the PbS@CdS@PEG QDs NPs were injected into the tumor-bearing mice for NIR-II fluorescence microscopic bioimaging. The brain vessels and tumors of the living mice were detected with high spatial resolution under the NIR-II fluorescence microscopic imaging system with irradiation of 808nm laser. The tumor tissues were further restricted and prepared as thin slices. The NIR-II fluorescence signals were collected from the tumor slices with high spatial resolution and signal-to-background ratio (SBR). Thus, the PbS@CdS@PEG QDs NPs-assisted NIR-II fluorescence microscopic system can effectively achieve targeting brain metastases of TNBC imaging, offering a novel and promising approach for TNBC-specific diagnosis.展开更多
Gastric cancer(GC)ranks among the most common and deadly malignancies globally.Surgical resection with lymph node(LN)dissection is the primary treatment.The accuracy of LN dissection is essential to reduce postoperati...Gastric cancer(GC)ranks among the most common and deadly malignancies globally.Surgical resection with lymph node(LN)dissection is the primary treatment.The accuracy of LN dissection is essential to reduce postoperative complications and mortality.Therefore,improving the quality of LN dissection in GC surgery and refining postoperative LN staging have been the focus of clinical attention.Indocyanine green(ICG)fluorescence imaging serves as a vital clinical tracing technique in GC surgery.It enables accurate tumor localization,enhances the completeness of LN dissection,and evaluates anastomotic blood supply after digestive tract reconstruction.These benefits collectively improve surgical outcomes and lower recurrence rates.This article examines the principles of ICG fluorescence imaging and its necessity in GC tracing surgery.Compared to conventional tracers,ICG offers superior safety and lower toxicity,with robust evidence supporting its clinical efficacy.This technology represents a paradigm shift in GC surgery.Current studies optimize ICG delivery protocols,such as injection time and dose,and integrate it with emerging technologies like robotic systems to improve LN detection rates.This article demonstrates the safety and efficacy of ICG as a tracer,which is poised to advance the precision of GC surgery and improve patient outcomes.展开更多
Non-invasive measurement of the transmission matrix(TM)enables light focusing through scattering media using linear fluorescence signals.However,its speed is limited by the frame rate of the array detector.Although ph...Non-invasive measurement of the transmission matrix(TM)enables light focusing through scattering media using linear fluorescence signals.However,its speed is limited by the frame rate of the array detector.Although photodetectors can achieve fast light focusing,they can only detect single-point signals and are ineffective in scenarios that require speckle information,such as non-invasive focusing.展开更多
Photodynamic therapy(PDT)presents a promising avenue in cancer treatment.Erlotinib,an FDAapproved anticancer drug targeting epidermal growth factor receptor(EGFR),has shown effectiveness in normalizing tumor vasculatu...Photodynamic therapy(PDT)presents a promising avenue in cancer treatment.Erlotinib,an FDAapproved anticancer drug targeting epidermal growth factor receptor(EGFR),has shown effectiveness in normalizing tumor vasculature across various tumors,thereby promoting tumor oxygenation and facilitating PDT.In this work,erlotinib was conjugated with a near-infrared(NIR)photosensitizer,benzo[a]phenoselenazinium,yielding three EGFR-targeted PDT agents(NBSe-n C-Er).These newly synthesized photosensitizers demonstrate specificity in binding to EGFR,thereby enhancing their accumulation in cancer cells and tumors,and consequently improving the efficiency of both PDT and chemotherapy.Additionally,the NIR fluorescence emitted by the photosensitizer allows for imaging-guided therapy,offering a non-invasive means of monitoring treatment progress.The distinctive properties of the three-inone photosensitizer render it an ideal candidate for precise tumor treatment,overcoming the limitations of conventional therapies.展开更多
Cancer is one of the main causes of death throughout the world.Radical elimination of tumor is crucial for a successful treatment.However,during cancer treatment,it is difficult to distinguish tumor boundaries with th...Cancer is one of the main causes of death throughout the world.Radical elimination of tumor is crucial for a successful treatment.However,during cancer treatment,it is difficult to distinguish tumor boundaries with the naked eye and to accurately exterminate it.In this work,based on the overexpression of H2S in some tumors,an activatable second near-infrared(NIR-Ⅱ)theranostic agent(NRS)for distinguishing tumor tissues from normal tissues,guiding surgical resection and ablating tumor tissues by efficient photothermal therapy is proposed.This developed probe NRS can emit fluorescence in the range of 900-1100 nm and detect tumor tissues with H2S overexpression.Under the guidance of NIR-Ⅱ fluorescence imaging,the tumor margins can be delineated clearly with high signal-to-background ratio.In addition,with the help of NIR-Ⅱ fluorescence surgery navigation,tumors tissues can be precisely resected.More importantly,the probe displays a high photothermal conversion efficiency and can efficiently induce tumor cells apoptosis under 808 nm laser irradiation.By using the desirable attributes of NRS,the tumor tissues with H2S overexpression was successfully ablated.This work provides a new tool for the future precision eradicate tumors without recurrence,which may have translational potential in biological and clinical systems.展开更多
Three-dimensional (3D) visualization of dynamic biological processes in deep tissue remains challenging due to the trade-off between temporal resolution and imaging depth. Here, we present a novel near-infrared-II (NI...Three-dimensional (3D) visualization of dynamic biological processes in deep tissue remains challenging due to the trade-off between temporal resolution and imaging depth. Here, we present a novel near-infrared-II (NIR-II, 900–1880nm) fluorescence volumetric microscopic imaging method that combines an electrically tunable lens (ETL) with deep learning approaches for rapid 3D imaging. The technology achieves volumetric imaging at 4.2 frames per second (fps) across a 200 μm depth range in live mouse brain vasculature. Two specialized neural networks are utilized: a scale-recurrent network (SRN) for image enhancement and a cerebral vessel interpolation (CVI) network that enables 16-fold axial upsampling. The SRN, trained on two-photon fluorescence microscopic data, improves both lateral and axial resolution of NIR-II fluorescence wide-field microscopic images. The CVI network, adapted from video interpolation techniques, generates intermediate frames between acquired axial planes, resulting in smooth and continuous 3D vessel reconstructions. Using this integrated system, we visualize and quantify blood flow dynamics in individual vessels and are capable of measuring blood velocity at different depths. This approach maintains high lateral resolution while achieving rapid volumetric imaging, and is particularly suitable for studying dynamic vascular processes in deep tissue. Our method demonstrates the potential of combining optical engineering with artificial intelligence to advance biological imaging capabilities.展开更多
BACKGROUND Intraoperative and postoperative biliary injuries remain significant complications of laparoscopic common bile duct exploration(LCBDE).Indocyanine green(ICG)has been shown to significantly reduce injuries c...BACKGROUND Intraoperative and postoperative biliary injuries remain significant complications of laparoscopic common bile duct exploration(LCBDE).Indocyanine green(ICG)has been shown to significantly reduce injuries caused by intraoperative operational errors.We found that the J-tube can reduce postoperative strictures and injuries to the common bile duct.At this moment,we aim to analyze and compare the complications,efficacy,short-term outcomes,and feasibility of these two adjunctive tools for LCBDE.AIM To evaluate the efficacy of ICG fluorescence imaging In LCBDE and J-tube drainage for patients with common bile duct stones.METHODS We retrospectively collected the clinical case data of patients who were treated at the Hepatobiliary Surgery Department of the Third People’s Hospital of Nantong,affiliated with Nantong University,from January 2016 to January 2021 due to gallbladder stones with choledocholithiasis and who underwent LCBDE combined with a primary suture and either J-tube or T-tube drainage.The patients were divided into groups:Traditional white-light laparoscopy+T-tube group(WL+T-tube),traditional WL+J-tube group,fluorescent laparoscopy+T-tube group(ICG+T-tube)and fluorescent laparoscopy+J-tube group(ICG+J-tube).The preoperative and postoperative clinical case data,laboratory examination data,and intraoperative and postoperative complications(including postoperative bile leakage,electrolyte disturbances,biliary peritonitis,and postoperative infections)and other relevant indicators were compared.RESULTS A total of 198 patients(112 males and 86 females)were included in the study,with 74 patients in the WL+T-tube,47 in the WL+J-tube,42 in the ICG+T-tube,and 35 in the ICG+J-tube.Compared with the other groups,the ICG+J had significantly shorter operation time(114 minutes,P=0.001),less blood loss(42 mL,P=0.02),shorter postoperative hospital stays(7 days,P=0.038),and lower surgical costs(China yuan 30178,P=0.001).Furthermore,patients were subdivided into two groups based on whether a T-tube or J-tube was placed during the surgery.By the third postoperative day,the aspartate transaminase,glutamic pyruvic transaminase,total bilirubin,and direct bilirubin levels were lower in the J-tube group than in the T-tube group(P<0.001).At last,follow-up observations showed that the incidence of biliary strictures at three months postoperatively was significantly lower in the J-tube group than in the T-tube group(P=0.002).CONCLUSION ICG fluorescence imaging in laparoscopic cholecystectomy with common bile duct exploration and J-tube drainage facilitates rapid identification of biliary anatomy and variations,reducing intraoperative bile duct injury,blood loss,surgery duration,and postoperative bile duct stenosis rates,supporting its clinical adoption.展开更多
A pancreas surgeon’s constant goal is to do"less damage,more radical".Currently,a small number of highly trained surgeons opt for single-incision laparoscopic pancreaticoduodenectomy(SILPD)or single-incisio...A pancreas surgeon’s constant goal is to do"less damage,more radical".Currently,a small number of highly trained surgeons opt for single-incision laparoscopic pancreaticoduodenectomy(SILPD)or single-incision plus one-port LPD(SILPD+1)to minimize post-operative pain,improve convalescence,and provide a more pleas-ing cosmetic outcome[1,2].Additionally,some skilled surgeons have claimed that laparoscopic duodenum-preserving complete pancreatic head resections(LDPPHR)result in less trauma and en-hanced quality of life[3,4].However,LDPPHR is still challenging because of its lengthy learning curve and"sword-fighting"impact.Additionally,there has not been any global reporting on the suit-ability of single-incision plus one-port DPPHR with pancreaticogas-trostomy(SILDPPHR-T+1)in place of SILPD+1.This study aimed to illustrate the SILDPPHR-T+1 procedure specifics for a patient with pancreatic head intraductal papillary mucinous neoplasm(IPMN)(main pancreatic duct type)(MD-IPMN).展开更多
Infrared and visible light images can be obtained simultaneously by building fluorescence imaging system,which includes fluorescence excitation,images acquisition,mechanical part,image transmission and processing sect...Infrared and visible light images can be obtained simultaneously by building fluorescence imaging system,which includes fluorescence excitation,images acquisition,mechanical part,image transmission and processing section.This system studied the 2charge-coupled device(CCD)camera(AD-080CL)of the JAI company.Fusion algorithm of visible light and near infrared images was designed for the fluorescence imaging system with wavelet transform image fusion algorithm.In order to enhance the fluorescent moiety of the fusion image,the luminance value of the green component of the color image was changed.And using microsoft foundation classes(MFC)application architecture,the supporting software system was bulit in VS2010 environment.展开更多
[Objective] This study was to determine the response of Ficus microcarpa L. foliage to polyethylene glycol （PEG） simulated water stress using chlorophyll fluo- rescence imaging technique. [Method] The responses of d...[Objective] This study was to determine the response of Ficus microcarpa L. foliage to polyethylene glycol （PEG） simulated water stress using chlorophyll fluo- rescence imaging technique. [Method] The responses of detached leaves from Ficus microcarpa, Ficus benjamina and Nerium oleander to PEG-6000 simulated water stress were detected, and the chlorophyll fluorescence imaging technique was used to detect and analyze the stress at different spots of a single leaf simultaneously. [Result] The responses of Ficus microcarpa, Ficus benjamina and Nerium oleander to dehydration showed that： ~1~） the maximal photochemical efficiency （Fv/Fm） and non- photo-chemical quenching （NPQ） values were small in the reaction center among different detected spots of leaves, and there were great differences between relative electron transport rate （ETR）, photochemical quenching （qP） and quantum efficiency of PSII photochemistry （（φPSII）; （2） the differences of these parameters were more ob- vious among different spots of water-stressed leaves; （3） the discrete degrees of the species with strong resitances decreased significantly. [Conclusion] This study lays the foundation for the further research on the response of plants to drought stress using chlorophyll fluorescence imaging technique.展开更多
The aim of this research was to study the influence of chlorsulfuron residue and cadmium on the enzymatic activity and photosynthetic apparatus of maize(Zea mays L.) plants. Chlorsulfuron and cadmium at 0.001 and 5.0 ...The aim of this research was to study the influence of chlorsulfuron residue and cadmium on the enzymatic activity and photosynthetic apparatus of maize(Zea mays L.) plants. Chlorsulfuron and cadmium at 0.001 and 5.0 mg kg–1, respectively, were mixed and applied to soil prior to planting. The levels of chlorsulfuron-and cadmium-induced stress to plants were estimated by growth, chlorophyll content, lipid peroxide content, enzyme activities, and major fluorescence parameters of chlorophyll(revealed by the fluorescence imaging system Fluor Cam). Chlorsulfuron negatively affected the chlorophyll content, photochemical efficiency of photosystem II in the dark-adapted state, the maximum efficiency of photosystem II, photochemical quenching coefficient, and steady-state fluorescence decline ratio in the leaves of maize seedlings. However, cadmium did not produce noticeable changes. Plants that were exposed to both chlorsulfuron and cadmium showed an obvious increase in the steady-state fluorescence decline ratio. These results implied that the seedlings possessed more resistance to cadmium than to chlorsulfuron and their resistance to chlorsulfuron toxicity was enhanced by the presence of cadmium. The results also suggested that chlorophyll fluorescence imaging reveals overall alterations within the leaves but may not reflect small-scale effects on tissues, as numeric values of specific parameters are averages of the data collected from the whole leaf.展开更多
Objective: To evaluate the imaging potential of a novel near-infrared(NIR) probe conjugated to COC183 B2 monoclonal antibodies(MAb) in ovarian cancer(OC).Methods: The expression of OC183 B2 antigen in OC was determine...Objective: To evaluate the imaging potential of a novel near-infrared(NIR) probe conjugated to COC183 B2 monoclonal antibodies(MAb) in ovarian cancer(OC).Methods: The expression of OC183 B2 antigen in OC was determined by immunohistochemical(IHC) staining using tissue microarrays with the H-score system and immunofluorescence(IF) staining of tumor cell lines.Imaging probes with the NIR fluorescent dye cyanine 7(Cy7) conjugated to COC183 B2 Mab were chemically engineered. OC183 B2-positive human OC cells(SKOV3-Luc) were injected subcutaneously into BALB/c nude mice. Bioluminescent imaging(BLI) was performed to detect tumor location and growth. COC183 B2-Cy7 at 1.1,3.3, 10, or 30 μg were used for in vivo fluorescence imaging, and phosphate-buffered saline(PBS), free Cy7 dye and mouse isotype immunoglobulin G(IgG)-Cy7(delivered at the same doses as COC183 B2-Cy7) were used as controls.Results: The expression of OC183 B2 with a high H-score was more prevalent in OC tissue than fallopian tube(FT) tissue. Among 417 OC patients, the expression of OC183 B2 was significantly correlated with the histological subtype, histological grade, residual tumor size, relapse state and survival status. IF staining demonstrated that COC183 B2 specifically expressed in SKOV3 cells but not HeLa cells. In vivo NIR fluorescence imaging indicated that COC183 B2-Cy7 was mainly distributed in the xenograft and liver with optimal tumor-to-background(T/B)ratios in the xenograft at 30 μg dose. The highest fluorescent signals in the tumor were observed at 96 h postinjection(hpi). Ex vivo fluorescence imaging revealed the fluorescent signals mainly from the tumor and liver. IHC analysis confirmed that xenografts were OC183 B2 positive.Conclusions: COC183 B2 is a good candidate for NIR fluorescence imaging and imaging-guided surgery in OC.展开更多
In recent years,the use of fluorescence-guided surgery(FGS)to treat benign and malignant visceral,hepatobiliary and pancreatic neoplasms has significantly increased.FGS relies on the fluorescence signal emitted by inj...In recent years,the use of fluorescence-guided surgery(FGS)to treat benign and malignant visceral,hepatobiliary and pancreatic neoplasms has significantly increased.FGS relies on the fluorescence signal emitted by injected substances(fluorophores)after being illuminated by ad hoc laser sources to help guide the surgical procedure and provide the surgeon with real-time visualization of the fluorescent structures of interest that would be otherwise invisible.This review surveys and discusses the most common and emerging clinical applications of indocyanine green(ICG)-based fluorescence in visceral,hepatobiliary and pancreatic surgery.The analysis,findings,and discussion presented here rely on the authors'significant experience with this technique in their medical institutions,an up-to-date review of the most relevant articles published on this topic between 2014 and 2018,and lengthy discussions with key opinion leaders in the field during recent conferences and congresses.For each application,the benefits and limitations of this technique,as well as applicable future directions,are described.The imaging of fluorescence emitted by ICG is a simple,fast,relatively inexpensive,and harmless tool with numerous different applications in surgery for both neoplasms and benign pathologies of the visceral and hepatobiliary systems.The ever-increasing availability of visual systems that can utilize this tool will transform some of these applications into the standard of care in the near future.Further studies are needed to evaluate the strengths and weaknesses of each application of ICG-based fluorescence imaging in abdominal surgery.展开更多
A new fluorescent probe（Rhod-Sec） for selenol detection with ultralow background fluorescence have been developed in this paper, which showed a 380-fold off-on fluorescence response, and the nontoxic Rhod-Sec is wel...A new fluorescent probe（Rhod-Sec） for selenol detection with ultralow background fluorescence have been developed in this paper, which showed a 380-fold off-on fluorescence response, and the nontoxic Rhod-Sec is well suitable for detecting and imaging both exogenous and endogenous selenol in living cells. It also can be applied to visualize the fluctuation of selenol in HepG2 cells through LPS-induced cells oxidation resistance.展开更多
The rapid development of super-resolution microscopy has made it possible to observe subcellular structures and dynamic behaviors in living cells with nanoscale spatial resolution, greatly advancing progress in life s...The rapid development of super-resolution microscopy has made it possible to observe subcellular structures and dynamic behaviors in living cells with nanoscale spatial resolution, greatly advancing progress in life sciences. As hardware technology continues to evolve, the availability of new fluorescent probes with superior performance is becoming increasingly important. In recent years, fluorescent nanoprobes (FNPs) have emerged as highly promising fluorescent probes for bioimaging due to their high brightness and excellent photostability. This paper focuses on the development and applications of FNPs as probes for live-cell super-resolution imaging. It provides an overview of different super-resolution methods, discusses the performance requirements for FNPs in these methods, and reviews the latest applications of FNPs in the super-resolution imaging of living cells. Finally, it addresses the challenges and future outlook in this field.展开更多
An ultimate goal of neuroscience is to decipher the principles underlying neuronal information processing at the molecular,cellular,circuit,and system levels.The advent of miniature fluorescence microscopy has further...An ultimate goal of neuroscience is to decipher the principles underlying neuronal information processing at the molecular,cellular,circuit,and system levels.The advent of miniature fluorescence microscopy has furthered the quest by visualizing brain activities and structural dynamics in animals engaged in self-determined behaviors.In this brief review,we summarize recent advances in miniature fluorescence microscopy for neuroscience,focusing mostly on two mainstream solutions-miniature single-photon microscopy,and miniature two-photon microscopy.We discuss their technical advantages and limitations as well as unmet challenges for future improvement.Examples of preliminary applications are also presented to reflect on a new trend of brain imaging in experimental paradigms involving body movements,long and complex protocols,and even disease progression and aging.展开更多
基金supported by the National Natural Science Foundation of China(Nos.82372116,U23A2097)Guangdong Basic and Applied Basic Research Foundation(No.2022A1515010620)+2 种基金Shenzhen Medical Research Fund(Nos.B2302047,A2302047)Shenzhen Science and Technology Program(No.JCYJ20220818095806014)Research Team Cultivation Program of Shenzhen University(No.2023QNT019).
文摘The level of glutathione(GSH)is significantly associated with numerous pathological processes,thus,real-time detection of the GSH level is of significance for early diagnosis of GSH-related diseases.Herein,we developed in vivo second near-infrared(NIR-II)window fluorescence(FL)and ratiometric photoacoustic(RPA)dual-modality imaging of GSH using a GSH-activatable probe(LET-14).LET-14 was synthesized based on a rhodamine hybrid xanthene skeleton with a FL shielding 2,4-dinitrobenzene sulfonyl group that can be specifically cleaved by GSH,thus resulting in a markedly bathochromic-shift absorption,a 6.5-fold increase in NIR-II FL intensity(FL920)and a 13-fold increase in RPA signal(PA880/PA705)in vitro.Intriguingly,LET-14 exhibits good selectivity and sensitivity for NIR-II FL and RPA dual-modality imaging of GSH in 4T1 tumor-bearing mouse model.Our findings develop an in vivo detection tool of GSH,which has great potential in the field of cancer diagnosis.
基金supported by the Natural Science Foundation of Hubei Province (Nos.2023AFB376 and 2024AFD287)National Key Research and Development Program (No.2023YFC3503804)the National Natural Science Foundation of China (No.22077044)。
文摘Sulfur dioxide(SO_(2)) and its derivatives have been recognized as harmful environmental pollutants.However,they are often produced during the processing of traditional Chinese medicines,potentially compromising the quality of these medicinal materials and contributing to various health issues.Due to a lack of effective monitoring and imaging tools,the physiological effects of excessive SO_(2) residues in traditional Chinese medicine remain unclear.Therefore,developing a rapid and effective tool for detecting SO_(2) is crucial for understanding its metabolic pathways and effects in vivo.In this study,we developed a near infrared(NIR) and ratiometric fluorescent probe,NIR-RS,which exhibits high sensitivity,selectivity,and rapid response for SO_(2) detection.Notably,NIR-RS accurately quantifies SO_(2) contents in Pinelliae rhizoma(P.rhizoma) samples,with recovery rates from 98.46 % to 102.40 %,and relative standard deviations(RSDs)< 5.0 %.For bioimaging applications,NIR-RS has low cytotoxicity and good mitochondrial-targeting ability,making it suitable for imaging exogenous and endogenous SO_(2) in mitochondria.Additionally,NIR-RS was successfully applied to image SO_(2) content of P.rhizoma samples within cells,revealing that high SO_(2) residue elevated mitochondria adenosine triphosphate(ATP) content,these findings reveal that P.rhizoma with excessive SO_(2) can affect the organism's growth mechanisms through alterations in ATP pathways.In vivo,SO_(2) was found to predominantly accumulate in the liver following gavage with P.rhizoma solution,with accumulation levels increasing in proportion to SO_(2) residue concentration.High SO_(2) concentrations in P.rhizoma can cause pulmonary fibrosis and gastric mucosal damage.This work provides a valuable tool for regulating SO_(2) content in P.rhizoma and may help researcher better understand the metabolism of SO_(2) derivatives and explore their physiological roles in biological systems.
基金financially supported by the Natural Science Foundation of Jiangsu Province(Grant No.BK20241181)the State Key Laboratory of AnalyticalChemistry for Life Science,School of Chemistry and Chemical Engineering,Nanjing University(Grant No.SKLACLS2419)。
文摘Using 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran(TCF)as a near-infrared fluorescent chromophore,we designed and synthesized a TCF-based fluorescent probe TCF-NS by introducing 2,4-dinitrophenyl ether as the recognized site for H_(2)S.The probe TCF-NS displayed a rapid-response fluorescent against H_(2)S with high sensitivity and selection but had no significant fluorescence response to other biothiols.Furthermore,TCF-NS was applied to sense H_(2)S in living cells successfully with minimized cytotoxicity and a large Stokes shift.
文摘In this editorial,we comment on the article by Li et al.We specifically focus on the novel use of multicolor near-infrared fluorescence imaging(MCFI)with indocyanine green in laparoscopic cholecystectomy,which is an innovative approach for enhancing biliary visualization during laparoscopic cholecystectomy.This study also highlighted the limitations of conventional single-color fluorescence imaging(SCFI),which relies solely on a green fluorescence signal,leading to challenges such as visual fatigue and difficulty in distinguishing biliary structures from background hepatic tissue.Given the complex anatomy of the biliary system and the challenges of visual fatigue encountered with SCFI,MCFI addresses these issues by enabling the differentiation of biliary structures by mapping the fluorescence intensity across a unique blue-to-purple color spectrum,thus improving the clarity of anatomical structures and reducing the visual strain for surgeons.We also focus specifically on the complications and cautious usage of indocyanine green in this context,as well as the advantages and disadvantages of MCFI and SCFI.Overall,MCFI represents a significant advancement in fluorescence-guided surgery,with the potential to become a standard imaging modality for safer and more effective laparoscopic procedures.
基金supported by the National Natural Science Foundation of China(NSFC)under Grant Nos.62035011,82202220 and 82060326State Key Laboratory of Pathogenesis,Prevention and treat ment of High Incident Diseases in central Asia(Nos.SKL-HIDCA-2022-3 and SKL-HIDCA-2022-GJ1)+3 种基金the Xinjiang Uygur Autonomous Region Regional Collaborative Innovation Special Science and Technology Assistance Program(No.2022E02130)Xinjiang Uygur Autonomous Region Natural Sci ence Foundation Key Project(No.2022D01D40)Outstanding Youth Project(2023D01E06)Y.Gao and C.Zhang authors contributed equally to this work.
文摘Triple-negative breast cancer (TNBC) is an aggressive and often fatal disease, especially since the brain metastasis of TNBC has been a particularly severe manifestation. However, brain metastasis in TNBC at early stages often lacks noticeable symptoms, making it challenging to detect. Near-infrared II (NIR-II) fluorescence microscopic imaging obtains long wavelength, which enables reduced scattering, high spatial resolution and minimal autofluorescence, it is also a favorable imaging method for tumor diagnosis. PbS@CdS quantum dots (QDs) are one of the popular NIR-II fluorescence nanoprobes for well brightness. In this study, NIR-II emissive PbS@CdS QDs were utilized and further encapsulated with thiol-terminated poly(ethylene oxide) (SH-PEG, MW = 5000) to form PbS@CdS@PEG QDs nanoparticles (NPs). The obtained PbS@CdS@PEG QDs NPs were then characterized and further studied in detail. The PbS@CdS@PEG QDs NPs had large absorption spectra, exhibited strong NIR-II fluorescence emission at approximately 1300nm, and possessed good NIR-II fluorescence properties. Then, the mice model of early-stage brain metastases of TNBC was established, and the PbS@CdS@PEG QDs NPs were injected into the tumor-bearing mice for NIR-II fluorescence microscopic bioimaging. The brain vessels and tumors of the living mice were detected with high spatial resolution under the NIR-II fluorescence microscopic imaging system with irradiation of 808nm laser. The tumor tissues were further restricted and prepared as thin slices. The NIR-II fluorescence signals were collected from the tumor slices with high spatial resolution and signal-to-background ratio (SBR). Thus, the PbS@CdS@PEG QDs NPs-assisted NIR-II fluorescence microscopic system can effectively achieve targeting brain metastases of TNBC imaging, offering a novel and promising approach for TNBC-specific diagnosis.
基金Supported by the National Natural Science Foundation of China,No.82473154 and No.82372805Jiangsu Province Basic Research Special Fund(Soft Science Research)Special Funding,No.BK20210022Nanjing Municipal Special Fund for Health Science and Technology Development,No.YKK21078.
文摘Gastric cancer(GC)ranks among the most common and deadly malignancies globally.Surgical resection with lymph node(LN)dissection is the primary treatment.The accuracy of LN dissection is essential to reduce postoperative complications and mortality.Therefore,improving the quality of LN dissection in GC surgery and refining postoperative LN staging have been the focus of clinical attention.Indocyanine green(ICG)fluorescence imaging serves as a vital clinical tracing technique in GC surgery.It enables accurate tumor localization,enhances the completeness of LN dissection,and evaluates anastomotic blood supply after digestive tract reconstruction.These benefits collectively improve surgical outcomes and lower recurrence rates.This article examines the principles of ICG fluorescence imaging and its necessity in GC tracing surgery.Compared to conventional tracers,ICG offers superior safety and lower toxicity,with robust evidence supporting its clinical efficacy.This technology represents a paradigm shift in GC surgery.Current studies optimize ICG delivery protocols,such as injection time and dose,and integrate it with emerging technologies like robotic systems to improve LN detection rates.This article demonstrates the safety and efficacy of ICG as a tracer,which is poised to advance the precision of GC surgery and improve patient outcomes.
基金National Natural Science Foundation of China(62275188)Central Guidance on Local Science and Technology Development Fund(YDZJSX2024D019)。
文摘Non-invasive measurement of the transmission matrix(TM)enables light focusing through scattering media using linear fluorescence signals.However,its speed is limited by the frame rate of the array detector.Although photodetectors can achieve fast light focusing,they can only detect single-point signals and are ineffective in scenarios that require speckle information,such as non-invasive focusing.
基金supported by the National Natural Science Foundation of China(Nos.22278447 and 22178395)State Key Laboratory of Fine Chemicals(No.KF2109)State Key Laboratory of Chemo/Biosensing and Chemometrics(No.20230768)。
文摘Photodynamic therapy(PDT)presents a promising avenue in cancer treatment.Erlotinib,an FDAapproved anticancer drug targeting epidermal growth factor receptor(EGFR),has shown effectiveness in normalizing tumor vasculature across various tumors,thereby promoting tumor oxygenation and facilitating PDT.In this work,erlotinib was conjugated with a near-infrared(NIR)photosensitizer,benzo[a]phenoselenazinium,yielding three EGFR-targeted PDT agents(NBSe-n C-Er).These newly synthesized photosensitizers demonstrate specificity in binding to EGFR,thereby enhancing their accumulation in cancer cells and tumors,and consequently improving the efficiency of both PDT and chemotherapy.Additionally,the NIR fluorescence emitted by the photosensitizer allows for imaging-guided therapy,offering a non-invasive means of monitoring treatment progress.The distinctive properties of the three-inone photosensitizer render it an ideal candidate for precise tumor treatment,overcoming the limitations of conventional therapies.
基金financially supported by National Natural Science Foundation of China(Nos.22406099,82172961,22077048 and22277014)the Special Fund of Taishan Scholars Project of Shandong Province(No.tsqnz20231253)+2 种基金Guangxi Natural Science Foundation(Nos.2021GXNSFDA075003,AD21220061)the startup fund of Guangxi University(No.A3040051003)the Major Science and Technology Plan Project of Hainan Province(No.ZDKJ202005)。
文摘Cancer is one of the main causes of death throughout the world.Radical elimination of tumor is crucial for a successful treatment.However,during cancer treatment,it is difficult to distinguish tumor boundaries with the naked eye and to accurately exterminate it.In this work,based on the overexpression of H2S in some tumors,an activatable second near-infrared(NIR-Ⅱ)theranostic agent(NRS)for distinguishing tumor tissues from normal tissues,guiding surgical resection and ablating tumor tissues by efficient photothermal therapy is proposed.This developed probe NRS can emit fluorescence in the range of 900-1100 nm and detect tumor tissues with H2S overexpression.Under the guidance of NIR-Ⅱ fluorescence imaging,the tumor margins can be delineated clearly with high signal-to-background ratio.In addition,with the help of NIR-Ⅱ fluorescence surgery navigation,tumors tissues can be precisely resected.More importantly,the probe displays a high photothermal conversion efficiency and can efficiently induce tumor cells apoptosis under 808 nm laser irradiation.By using the desirable attributes of NRS,the tumor tissues with H2S overexpression was successfully ablated.This work provides a new tool for the future precision eradicate tumors without recurrence,which may have translational potential in biological and clinical systems.
基金supported by the National Key R&D Program of China (No. 2024YFF1206700)the National Natural Science Foundation of China (No. U23A20487)the Hangzhou Chengxi Sci-tech Innovation Corridor Management Committee.
文摘Three-dimensional (3D) visualization of dynamic biological processes in deep tissue remains challenging due to the trade-off between temporal resolution and imaging depth. Here, we present a novel near-infrared-II (NIR-II, 900–1880nm) fluorescence volumetric microscopic imaging method that combines an electrically tunable lens (ETL) with deep learning approaches for rapid 3D imaging. The technology achieves volumetric imaging at 4.2 frames per second (fps) across a 200 μm depth range in live mouse brain vasculature. Two specialized neural networks are utilized: a scale-recurrent network (SRN) for image enhancement and a cerebral vessel interpolation (CVI) network that enables 16-fold axial upsampling. The SRN, trained on two-photon fluorescence microscopic data, improves both lateral and axial resolution of NIR-II fluorescence wide-field microscopic images. The CVI network, adapted from video interpolation techniques, generates intermediate frames between acquired axial planes, resulting in smooth and continuous 3D vessel reconstructions. Using this integrated system, we visualize and quantify blood flow dynamics in individual vessels and are capable of measuring blood velocity at different depths. This approach maintains high lateral resolution while achieving rapid volumetric imaging, and is particularly suitable for studying dynamic vascular processes in deep tissue. Our method demonstrates the potential of combining optical engineering with artificial intelligence to advance biological imaging capabilities.
文摘BACKGROUND Intraoperative and postoperative biliary injuries remain significant complications of laparoscopic common bile duct exploration(LCBDE).Indocyanine green(ICG)has been shown to significantly reduce injuries caused by intraoperative operational errors.We found that the J-tube can reduce postoperative strictures and injuries to the common bile duct.At this moment,we aim to analyze and compare the complications,efficacy,short-term outcomes,and feasibility of these two adjunctive tools for LCBDE.AIM To evaluate the efficacy of ICG fluorescence imaging In LCBDE and J-tube drainage for patients with common bile duct stones.METHODS We retrospectively collected the clinical case data of patients who were treated at the Hepatobiliary Surgery Department of the Third People’s Hospital of Nantong,affiliated with Nantong University,from January 2016 to January 2021 due to gallbladder stones with choledocholithiasis and who underwent LCBDE combined with a primary suture and either J-tube or T-tube drainage.The patients were divided into groups:Traditional white-light laparoscopy+T-tube group(WL+T-tube),traditional WL+J-tube group,fluorescent laparoscopy+T-tube group(ICG+T-tube)and fluorescent laparoscopy+J-tube group(ICG+J-tube).The preoperative and postoperative clinical case data,laboratory examination data,and intraoperative and postoperative complications(including postoperative bile leakage,electrolyte disturbances,biliary peritonitis,and postoperative infections)and other relevant indicators were compared.RESULTS A total of 198 patients(112 males and 86 females)were included in the study,with 74 patients in the WL+T-tube,47 in the WL+J-tube,42 in the ICG+T-tube,and 35 in the ICG+J-tube.Compared with the other groups,the ICG+J had significantly shorter operation time(114 minutes,P=0.001),less blood loss(42 mL,P=0.02),shorter postoperative hospital stays(7 days,P=0.038),and lower surgical costs(China yuan 30178,P=0.001).Furthermore,patients were subdivided into two groups based on whether a T-tube or J-tube was placed during the surgery.By the third postoperative day,the aspartate transaminase,glutamic pyruvic transaminase,total bilirubin,and direct bilirubin levels were lower in the J-tube group than in the T-tube group(P<0.001).At last,follow-up observations showed that the incidence of biliary strictures at three months postoperatively was significantly lower in the J-tube group than in the T-tube group(P=0.002).CONCLUSION ICG fluorescence imaging in laparoscopic cholecystectomy with common bile duct exploration and J-tube drainage facilitates rapid identification of biliary anatomy and variations,reducing intraoperative bile duct injury,blood loss,surgery duration,and postoperative bile duct stenosis rates,supporting its clinical adoption.
基金supported by grants from the National Natu-ral Science Foundation of China(81302161 and 82003103)the Science and Technology Department of Sichuan Province(2021YFS0375 and 2020YJ0450).
文摘A pancreas surgeon’s constant goal is to do"less damage,more radical".Currently,a small number of highly trained surgeons opt for single-incision laparoscopic pancreaticoduodenectomy(SILPD)or single-incision plus one-port LPD(SILPD+1)to minimize post-operative pain,improve convalescence,and provide a more pleas-ing cosmetic outcome[1,2].Additionally,some skilled surgeons have claimed that laparoscopic duodenum-preserving complete pancreatic head resections(LDPPHR)result in less trauma and en-hanced quality of life[3,4].However,LDPPHR is still challenging because of its lengthy learning curve and"sword-fighting"impact.Additionally,there has not been any global reporting on the suit-ability of single-incision plus one-port DPPHR with pancreaticogas-trostomy(SILDPPHR-T+1)in place of SILPD+1.This study aimed to illustrate the SILDPPHR-T+1 procedure specifics for a patient with pancreatic head intraductal papillary mucinous neoplasm(IPMN)(main pancreatic duct type)(MD-IPMN).
基金National Natural Science Foundation of China(No.61171177)National Major Scientific Equipment Development Projects of China(No.2013YQ240803)+1 种基金Natural Science Foundation for Young Scientists of Shanxi Province(No.2012021011-1)Scientific and Technological Project in Shanxi Province(No.20140321010-02)
文摘Infrared and visible light images can be obtained simultaneously by building fluorescence imaging system,which includes fluorescence excitation,images acquisition,mechanical part,image transmission and processing section.This system studied the 2charge-coupled device(CCD)camera(AD-080CL)of the JAI company.Fusion algorithm of visible light and near infrared images was designed for the fluorescence imaging system with wavelet transform image fusion algorithm.In order to enhance the fluorescent moiety of the fusion image,the luminance value of the green component of the color image was changed.And using microsoft foundation classes(MFC)application architecture,the supporting software system was bulit in VS2010 environment.
基金Supported by the Major Program for the West Action Projects of the Knowledge Innovation Program of the Chinese Academy of Sciences(KZCX2-XB2-08)the Science-Technology Foundation of Zealquest(ZQFD200705)~~
文摘[Objective] This study was to determine the response of Ficus microcarpa L. foliage to polyethylene glycol （PEG） simulated water stress using chlorophyll fluo- rescence imaging technique. [Method] The responses of detached leaves from Ficus microcarpa, Ficus benjamina and Nerium oleander to PEG-6000 simulated water stress were detected, and the chlorophyll fluorescence imaging technique was used to detect and analyze the stress at different spots of a single leaf simultaneously. [Result] The responses of Ficus microcarpa, Ficus benjamina and Nerium oleander to dehydration showed that： ~1~） the maximal photochemical efficiency （Fv/Fm） and non- photo-chemical quenching （NPQ） values were small in the reaction center among different detected spots of leaves, and there were great differences between relative electron transport rate （ETR）, photochemical quenching （qP） and quantum efficiency of PSII photochemistry （（φPSII）; （2） the differences of these parameters were more ob- vious among different spots of water-stressed leaves; （3） the discrete degrees of the species with strong resitances decreased significantly. [Conclusion] This study lays the foundation for the further research on the response of plants to drought stress using chlorophyll fluorescence imaging technique.
基金supported by grants from the National Natural Science Foundation of China(30740037)the Special Fund for Agro-scientific Research in the Public Interest,China(201103024)the Foundation for Graduate Innovation,Shanxi University,China(011452901009)
文摘The aim of this research was to study the influence of chlorsulfuron residue and cadmium on the enzymatic activity and photosynthetic apparatus of maize(Zea mays L.) plants. Chlorsulfuron and cadmium at 0.001 and 5.0 mg kg–1, respectively, were mixed and applied to soil prior to planting. The levels of chlorsulfuron-and cadmium-induced stress to plants were estimated by growth, chlorophyll content, lipid peroxide content, enzyme activities, and major fluorescence parameters of chlorophyll(revealed by the fluorescence imaging system Fluor Cam). Chlorsulfuron negatively affected the chlorophyll content, photochemical efficiency of photosystem II in the dark-adapted state, the maximum efficiency of photosystem II, photochemical quenching coefficient, and steady-state fluorescence decline ratio in the leaves of maize seedlings. However, cadmium did not produce noticeable changes. Plants that were exposed to both chlorsulfuron and cadmium showed an obvious increase in the steady-state fluorescence decline ratio. These results implied that the seedlings possessed more resistance to cadmium than to chlorsulfuron and their resistance to chlorsulfuron toxicity was enhanced by the presence of cadmium. The results also suggested that chlorophyll fluorescence imaging reveals overall alterations within the leaves but may not reflect small-scale effects on tissues, as numeric values of specific parameters are averages of the data collected from the whole leaf.
基金supported by the National Key Research and Development Program of China (No.2016YFA0201400)National Natural Science Foundation of China (No. 81671431)
文摘Objective: To evaluate the imaging potential of a novel near-infrared(NIR) probe conjugated to COC183 B2 monoclonal antibodies(MAb) in ovarian cancer(OC).Methods: The expression of OC183 B2 antigen in OC was determined by immunohistochemical(IHC) staining using tissue microarrays with the H-score system and immunofluorescence(IF) staining of tumor cell lines.Imaging probes with the NIR fluorescent dye cyanine 7(Cy7) conjugated to COC183 B2 Mab were chemically engineered. OC183 B2-positive human OC cells(SKOV3-Luc) were injected subcutaneously into BALB/c nude mice. Bioluminescent imaging(BLI) was performed to detect tumor location and growth. COC183 B2-Cy7 at 1.1,3.3, 10, or 30 μg were used for in vivo fluorescence imaging, and phosphate-buffered saline(PBS), free Cy7 dye and mouse isotype immunoglobulin G(IgG)-Cy7(delivered at the same doses as COC183 B2-Cy7) were used as controls.Results: The expression of OC183 B2 with a high H-score was more prevalent in OC tissue than fallopian tube(FT) tissue. Among 417 OC patients, the expression of OC183 B2 was significantly correlated with the histological subtype, histological grade, residual tumor size, relapse state and survival status. IF staining demonstrated that COC183 B2 specifically expressed in SKOV3 cells but not HeLa cells. In vivo NIR fluorescence imaging indicated that COC183 B2-Cy7 was mainly distributed in the xenograft and liver with optimal tumor-to-background(T/B)ratios in the xenograft at 30 μg dose. The highest fluorescent signals in the tumor were observed at 96 h postinjection(hpi). Ex vivo fluorescence imaging revealed the fluorescent signals mainly from the tumor and liver. IHC analysis confirmed that xenografts were OC183 B2 positive.Conclusions: COC183 B2 is a good candidate for NIR fluorescence imaging and imaging-guided surgery in OC.
文摘In recent years,the use of fluorescence-guided surgery(FGS)to treat benign and malignant visceral,hepatobiliary and pancreatic neoplasms has significantly increased.FGS relies on the fluorescence signal emitted by injected substances(fluorophores)after being illuminated by ad hoc laser sources to help guide the surgical procedure and provide the surgeon with real-time visualization of the fluorescent structures of interest that would be otherwise invisible.This review surveys and discusses the most common and emerging clinical applications of indocyanine green(ICG)-based fluorescence in visceral,hepatobiliary and pancreatic surgery.The analysis,findings,and discussion presented here rely on the authors'significant experience with this technique in their medical institutions,an up-to-date review of the most relevant articles published on this topic between 2014 and 2018,and lengthy discussions with key opinion leaders in the field during recent conferences and congresses.For each application,the benefits and limitations of this technique,as well as applicable future directions,are described.The imaging of fluorescence emitted by ICG is a simple,fast,relatively inexpensive,and harmless tool with numerous different applications in surgery for both neoplasms and benign pathologies of the visceral and hepatobiliary systems.The ever-increasing availability of visual systems that can utilize this tool will transform some of these applications into the standard of care in the near future.Further studies are needed to evaluate the strengths and weaknesses of each application of ICG-based fluorescence imaging in abdominal surgery.
基金supported by the National Natural Science Foundation of China (Nos. 21622504, 21302050)the Hunan Provincial Natural Science Foundation of China (Nos. 14JJ2047)+1 种基金the Hunan University Fund for Multidisciplinary Developing (Nos. 2015JCA04)Open Funding Project of the State Key Laboratory of Bioreactor Engineering
文摘A new fluorescent probe（Rhod-Sec） for selenol detection with ultralow background fluorescence have been developed in this paper, which showed a 380-fold off-on fluorescence response, and the nontoxic Rhod-Sec is well suitable for detecting and imaging both exogenous and endogenous selenol in living cells. It also can be applied to visualize the fluctuation of selenol in HepG2 cells through LPS-induced cells oxidation resistance.
基金supported by the following grants:National Natural Science Foundation of China(grant nos.92354305,32271428,and 32201132)National Key R&D Program of China(grant no.2022YFC3401100)+1 种基金Fund for Knowledge Innovation of Wuhan Science and Technology Bureau(grant no.2022020801010558)Director Fund of WNLO.
文摘The rapid development of super-resolution microscopy has made it possible to observe subcellular structures and dynamic behaviors in living cells with nanoscale spatial resolution, greatly advancing progress in life sciences. As hardware technology continues to evolve, the availability of new fluorescent probes with superior performance is becoming increasingly important. In recent years, fluorescent nanoprobes (FNPs) have emerged as highly promising fluorescent probes for bioimaging due to their high brightness and excellent photostability. This paper focuses on the development and applications of FNPs as probes for live-cell super-resolution imaging. It provides an overview of different super-resolution methods, discusses the performance requirements for FNPs in these methods, and reviews the latest applications of FNPs in the super-resolution imaging of living cells. Finally, it addresses the challenges and future outlook in this field.
基金We thank Dr.Zhe Zhao and Dr.Haitao Wu for helping with the experiments for Fig.2D,and Dr.Weijian Zong for discussion.This work was supported by grants from the National Natural Science Foundation of China(31327901,31570839,61975002,31830036,31821091,and 8182780030)the Major State Basic Research Program of China(2016 YFA0500400 and 2016YFA0500403)and the National Postdoctoral Program for Innovative Talents of China(BX20190011).
文摘An ultimate goal of neuroscience is to decipher the principles underlying neuronal information processing at the molecular,cellular,circuit,and system levels.The advent of miniature fluorescence microscopy has furthered the quest by visualizing brain activities and structural dynamics in animals engaged in self-determined behaviors.In this brief review,we summarize recent advances in miniature fluorescence microscopy for neuroscience,focusing mostly on two mainstream solutions-miniature single-photon microscopy,and miniature two-photon microscopy.We discuss their technical advantages and limitations as well as unmet challenges for future improvement.Examples of preliminary applications are also presented to reflect on a new trend of brain imaging in experimental paradigms involving body movements,long and complex protocols,and even disease progression and aging.
