Flowering time is a critical agronomic trait with a profound effect on the productivity and adaptabillity of rapeseed(Brassica napus L.).Strategically advancing flowering time can reduce the risk of yield losses due t...Flowering time is a critical agronomic trait with a profound effect on the productivity and adaptabillity of rapeseed(Brassica napus L.).Strategically advancing flowering time can reduce the risk of yield losses due to extreme climatic conditions and facilitate the cultivation of subsequent crops on the same land,thereby enhancing overall agricultural efficiency.In this review,we synthesize current information on flowering time regulation in rapeseed through an integrated analysis of its genetic,hormonal,and environmental dimensions,emphasizing their crosstalk and implications for yield.We consolidate multi-omics evidence from population genetics,functional genomics,and systems biology to create a haplotype-based framework that overcomes the trade-off between flowering time and yield,providing support for the precision breeding of early-maturing cultivars.The insights presented here could inform future research on flowering time regulation and guide strategies for increasing rapeseed productivity.展开更多
Objective Anoectochilus roxburghii is a valuable medicinal and ornamental plant.The aim of this study is to investigate the morphological and biochemical responses during the flower development stages of A.roxburghii,...Objective Anoectochilus roxburghii is a valuable medicinal and ornamental plant.The aim of this study is to investigate the morphological and biochemical responses during the flower development stages of A.roxburghii,and to assess the effects of exogenous polyamines(PAs)on bud differentiation and metabolism,thereby providing a theoretical basis for understanding the flowering form and physiology of A.roxburghii.Methods In this study,morphological and biochemical responses in flower development stages of A.roxburghii were investigated using paraffin sections and stereomicroscope.A.roxburghii was divided into five periods,including vegetative growth period,flower bud period,flowering period,late flowering period and fruiting period.During the flowering phase,specific biochemical parameters were measured,including soluble sugar content,superoxide dismutase(SOD)activity,soluble protein content,peroxidase(POD)activity,and catalase(CAT)activity.These measurements were conducted to understand the biochemical changes occurring within A.roxburghii during its flowering process.Furthermore,the effects of PAs on bud differentiation were examined.Additionally,the activities of S-adenosylmethionine decarboxylase(SAMDC)and polyamine oxidase(PAO),as well as the content of polyphenols,polysaccharides,and flavonoids in A.roxburghii,were measured after PA treatment to evaluate the metabolic changes induced by exogenous PAs.Results During the flowering phase of A.roxburghii,soluble sugar content and SOD activity were steadily declining.Soluble protein content was initially increasing and then reducing,and POD and CAT activities showed opposite pattern.In addition,the effects of exogenous PAs on bud differentiation were investigated.Results showed that 3 mmol/L putrescine or 0.3 mmol/L spermidine significantly promoted the bud differentiation of A.roxburghii and advanced the flowering.The activities of SAMDC,PAO,and the content of polyphenols,polysaccharides and flavonoids in A.roxburghii significantly increased after PA treatment,demonstrating that exogenous PA can accelerate metabolism and improved the active ingredients content.Conclusion The flower development of A.roxburghii was divided into five stages,with significant changes in soluble sugar,protein,POD,SOD,CAT,MDA,and PRO levels.Exogenous putrescine and spermidine enhanced bud differentiation and accelerated flowering,increasing SAMDC and PAO activities,suggesting accelerated PA metabolism.PAs also improved active component content.These findings provide a theoretical basis for studying flower morphology and PA-induced flowering regulation of A.roxburghii.展开更多
Flowering is one of the most important phenological periods,as it determines the timing of fruit maturation and seed dispersal.To date,both nitric oxide(NO)and DNA demethylation have been reported to regulate flowerin...Flowering is one of the most important phenological periods,as it determines the timing of fruit maturation and seed dispersal.To date,both nitric oxide(NO)and DNA demethylation have been reported to regulate flowering in plants.However,there is no compelling experimental evidence for a relationship between NO and DNA demethylation during plant flowering.In this study,an NO donor and a DNA methylation inhibitor were used to investigate the involvement of DNA demethylation in NO-mediated tomato(Solanum lycopersicum cv.Micro-Tom)flowering.The results showed that the promoting effect of NO on tomato flowering was dose-dependent,with the greatest positive effect observed at 10μmol L^(-1) of the NO donor S-nitrosoglutathione(GSNO).Treatment with 50μmol L^(-1) of the DNA methylation inhibitor 5-azacitidine(5-AzaC)also significantly promoted tomato flowering.Moreover,GSNO and 5-AzaC increased the peroxidase(POD)and catalase(CAT)activities and cytokinin(CTK)and proline contents,while they reduced the gibberellic acid(GA3)and indole-3-acetic acid(IAA)contents.Co-treatment with GSNO and 5-AzaC accelerated the positive effects of GSNO and 5-AzaC in promoting tomato flowering.Meanwhile,compared with a GSNO or 5-AzaC treatment alone,co-treatment with GSNO+5-AzaC significantly increased the global DNA demethylation levels in different tissues of tomato.The results also indicate that DNA demethylation may be involved in NO-induced flowering.The expression of flowering genes was significantly altered by the GSNO+5-AzaC treatment.Five of these flowering induction genes,ARGONAUTE 4(AGO4A),SlSP3D/SINGLE FLOWER TRUSS(SFT),MutS HOMOLOG 1(MSH1),ZINC FINGER PROTEIN 2(ZFP2),and FLOWERING LOCUS D(FLD),were selected as candidate genes for further study.An McrBC-PCR analysis showed that DNA demethylation of the SFT gene in the apex and the FLD gene in the stem might be involved in NO-induced flowering.Therefore,this study shows that NO might promote tomato flowering by mediating the DNA demethylation of flowering induction genes,and it provides direct evidence for a synergistic effect of NO and DNA demethylation in promoting tomato flowering.展开更多
FLOWERING LOCUS T(FT)基因是影响高等植物开花的关键基因,其翻译产生的蛋白产物是一种可长距离转运的成花激素,对开花时间起着重要的作用。目前在烟草种子生产中,均采用杂交育种方式,因此掌握烟草的开花时间尤为重要。为更好地了解FT...FLOWERING LOCUS T(FT)基因是影响高等植物开花的关键基因,其翻译产生的蛋白产物是一种可长距离转运的成花激素,对开花时间起着重要的作用。目前在烟草种子生产中,均采用杂交育种方式,因此掌握烟草的开花时间尤为重要。为更好地了解FT开花基因在烟草中的结构、表达及其进化关系,本研究利用8个烟草种的完整基因组序列数据,鉴定并分析了FT基因在8个烟草种中的蛋白结构、保守结构域、进化关系、共线性、基因注释和基因表达。结果显示,在8个烟草基因组中筛选鉴定出106个FT基因,并将这些FT基因分为6个组。在与其他植物FT基因的进化分析中发现野生烟草FT基因在进化过程中与茄科植物较接近。对普通烟草和2个亲本野生烟草的共线性分析,表明了普通烟草BX(Nicotiana tabacum BX)和普通烟草TN90(Nicotiana tabacum TN90)的FT基因进化关系非常接近。在FT基因注释分析中,发现有22个基因是促进烟草花朵发育,有16个基因是参与抑制花发育的生物学途径。所有106个FT基因均参与Ko04712植物昼夜节律代谢途径,并在其中担任开花途径的一部分。FT基因在普通烟草TN90的9个不同生长发育时期的表达模式分析发现,FT基因在根、茎、叶和花中均有表达,单表达量存在明显差异,其中大部分的FT基因是表达于鲜花朵、成熟花朵和衰老花朵3个时期中。这些结果将对以后研究中的基因查询、基因克隆和功能验证等方面发挥重要的作用。展开更多
Tibetan turnip and oilseeds are the most important vegetables cultivated in the Qinghai-Tibet Plateau.Our field observations identified a dominant early-bolting phenotype at the vegetative growth stage in the Tibetan ...Tibetan turnip and oilseeds are the most important vegetables cultivated in the Qinghai-Tibet Plateau.Our field observations identified a dominant early-bolting phenotype at the vegetative growth stage in the Tibetan turnip,which was possibly due to cross-pollination contamination from nearby oilseeds.We performed genetic and molecular experiments to explore the main reason for early bolting.We first analyzed gene expression and genomic sequence variation of turnip and oilseed BraFLC2,a gene that acts as a key repressor of flowering in turnip in a dosage-dependent manner.We found that the differences in flowering time and life habits between turnip and oilseeds were closely correlated with the genetic variations in BraFLC2.We further identified that the early-bolting turnip was the hybrid between turnip and oilseeds by selecting BraFLC2 as a testing gene.Furthermore,using an artificial hybridization experiment,we found that the heterozygote and low levels of BraFLC2 expression promoted early bolting in hybrid plants.These findings indicate that early-bolting in turnip is caused by cross-pollination contamination from oilseeds under human agricultural activities.We propose a strategy of strict seed screening,cultivation isolation and turnip breeding to ensure high quality and yield.展开更多
The proper flowering time of rose(Rosa hybrida)is vital for the market value of this horticultural crop,but the mechanism regulating this trait is largely unclear.Here,we found that the transcription factor SQUAMOSA P...The proper flowering time of rose(Rosa hybrida)is vital for the market value of this horticultural crop,but the mechanism regulating this trait is largely unclear.Here,we found that the transcription factor SQUAMOSA PROMOTER BINDING PROTEIN-LIKE4(RhSPL4)positively regulates flowering time in rose.Transient silencing or overexpression transgenic rose plants of RhSPL4 exhibited delayed or early flowering,respectively.Analysis of transcriptome data from transgenic lines overexpressing RhSPL4 compared to the wild type indicated that differentially expressed genes were significantly enriched in the circadian rhythm pathway.Among the proteins encoded by these genes,RhSPL4 binds to the promoter of PSEUDO-RESPONSE REGULATOR 5-LIKE(RhPRR5L),as revealed in yeast one-hybrid,dual-Luciferase/Renilla luciferase reporter,chromatin immunoprecipitation-quantitative PCR and electrophoretic mobility shift assay.Furthermore,RhSPL4 specifically binds to the478 to441 bp region of the RhPRR5L promoter and activates its transcription.The silencing of RhPRR5L delayed flowering time in rose,resembling the phenotype of RhSPL4-silenced plants.Together,these results indicate that the RhSPL4-RhPRR5L module positively regulates flowering time in rose,laying the foundation for the genetic improvement of flowering time in this important horticultural crop.展开更多
Nitrogen(N)is a key component in plants and their biological macromolecules,having a profound effect on developmental stages,such as germination,vegetative growth,and flowering.However,the mechanism of nitrogen-regula...Nitrogen(N)is a key component in plants and their biological macromolecules,having a profound effect on developmental stages,such as germination,vegetative growth,and flowering.However,the mechanism of nitrogen-regulated flowering time remains unclear.In this study,CmNLP7 was isolated from the chrysanthemum cultivar‘Jinba'and was characterized.CmNLP7 is a transcription factor localized in the nucleus but has no transcriptional activity.Tissue expression pattern analysis showed that CmNLP7 was mainly transcribed in leaves and roots.Knocking down CmNLP7 through the artificial-miRNA method in chrysanthemum resulted in early flowering under optimal nitrogen(ON)and low nitrogen(LN)conditions;whereas overexpression lines showed delayed flowering under LN conditions.Transcriptome sequencing analysis showed that the nitrate transporters NRT2.5,NPF3.1,and NPF4.6;SBP-like genes SPL7 and SPL12,and flowering integration factor FT were significantly up-regulated in the knockdown lines.Based on the KEGG pathway enrichment analysis,the differentially transcribed genes were enriched in phenylpropanoid biosynthesis and starch and sucrose metabolism pathways,which indicated their alleged function in nitrogen-regulated flowering and development in chrysanthemum.Furthermore CmPP6 as a homolog of the Arabidopsis phosphatase PP6,was verified as an interacting protein of CmNLP7 by yeast two-hybrid,BiFC,pull-down and Biacore in vitro and in vivo,and the knockdown line of CmPP6(amiR-CmPP6)flowered earlier compared to that of the wild-type chrysanthemum‘Jinba'.Collectively,these results demonstrated that CmPP6 interacts with CmNLP7 to regulate chrysanthemum flowering,and CmNLP7 could regulate flowering time in response to nitrogen,which lays a foundation for the regulation of flowering and molecular breeding of chrysanthemum through changes in nutrient signaling.展开更多
The peony has become a cultural and economic powerhouse in Heze.The peony,revered as the“king of flowers”in China,has long transcended its botanical identity to become a cultural icon deeply rooted in the Yellow Riv...The peony has become a cultural and economic powerhouse in Heze.The peony,revered as the“king of flowers”in China,has long transcended its botanical identity to become a cultural icon deeply rooted in the Yellow River basin.In the fertile plains of the Yellow River,Heze City in Shandong Province has long held a special connection with the peony.展开更多
In plants,numerous non-Mendelian inherited dominant effects,including over-,incomplete-,and codominance,are frequently observed,yet they remain insufficiently understood.A novel phenotype has been identified in specif...In plants,numerous non-Mendelian inherited dominant effects,including over-,incomplete-,and codominance,are frequently observed,yet they remain insufficiently understood.A novel phenotype has been identified in specific soybean transformants overexpressing a single 35S::GmFT2a copy:superearly flowering dominance is exclusively observed in hemizygotes,not in homozygotes.Homozygous individual exhibits si RNA-mediated DNA methylation,causing epigenetic transcriptional silencing,whereas no such effect occurs in hemizygotes.Intriguingly,two distinct rounds of DNA methylation establishment occur,each mediated by a different mechanism.The homozygotes that derived from the hemizygous mother plants carrying 35S::GmFT2a locus was associated with the initiation of CHHcontext DNA methylation at 35S promoters mediated by 21 and 22 nucleotide(nt)si RNAs.Subsequently,24 nt si RNAs contribute to additional CHG-and CG-context DNA methylation at 35S promoters during the homozygosity of genes in plants already homozygous in maternal lineage.Reducing DNA methylation levels can be achieved by generating a hemizygous genotype through a crossing experiment with a recessive genotype.This research has unveiled a phenomenon:hemizygote-dependent dominance resulting from transcriptional silencing in homozygote offsprings.It provides new insights into the molecular mechanism underlying dominant effects.展开更多
Moso bamboo holds significant economic importance in China,serving various purposes,such as food,material,ornamentation,and greenery.Despite its versatility,the occurrence of flowering in Moso bamboo poses a threat to...Moso bamboo holds significant economic importance in China,serving various purposes,such as food,material,ornamentation,and greenery.Despite its versatility,the occurrence of flowering in Moso bamboo poses a threat to bamboo forests,resulting in substantial losses.The underlying cause of bamboo flowering remains elusive.Dynamic fluctuations in DNA methylation govern the transcriptional levels of crucial genes pivotal for plant growth and development.In this study,we conducted comprehensive DNA methylation(by whole-genome bisulfite sequencing)and transcriptome(by RNA-seq)analyses on non-flowering leaves,flowering leaves,and spikelets of Moso bamboo.Our findings revealed a notable reduction in the overall DNA methylation level,particularly CHH methylation,from leaves to spikelets,influencing the expression of differentially regulated genes.Notably,we identified DNA methylation as a regulatory mechanism for numerous flowering-related genes,including SPLs,FT,and SOC1.Specifically,the SPL3f gene,a key regulator of the aging pathway,exhibited hypomethylation and a high expression level in spikelets.Conversely,SOC1c displayed transcriptional silencing attributed to hypermethylation in the CHH context in the leaves of non-flowering plants.DNA methylation may affect the flowering mechanism of Moso bamboo by regulating the expression of key genes.In summary,our results shed light on the dynamic changes in DNA methylation between leaves and spikelets,unraveling an important epigenetic modification mechanism for flowering in Moso bamboo.展开更多
Appropriate flowering time in rapeseed(Brassica napus L.)is vital for preventing losses from weather,diseases,and pests.However,the molecular basis of its regulation remains largely unknown.Here,a genome-wide associat...Appropriate flowering time in rapeseed(Brassica napus L.)is vital for preventing losses from weather,diseases,and pests.However,the molecular basis of its regulation remains largely unknown.Here,a genome-wide association study identifies BnaC09.FUL,a MADS-box transcription factor,as a promising candidate gene regulating flowering time in B.napus.BnaC09.FUL expression increases sharply in B.napus shoot apices near bolting.BnaC09.FUL overexpression results in early flowering,while BnaFUL mutation causes delayed flowering in B.napus.A zinc finger transcription factor,BnaC06.WIP2,is identified as an interaction partner of BnaC09.FUL,and BnaC06.WIP2 overexpression delays flowering in B.napus,with RNA sequencing revealing its influence on the expression of many flowering-associated genes.We further demonstrate that BnaC06.WIP2 directly represses the expression of BnaA05.SOC1,BnaC03.SOC1,BnaC04.SOC1,BnaC06.FT,BnaA06.LFY,BnaC07.FUL,BnaA08.CAL,and BnaC03.CAL and indirectly inhibits the expression of other flowering time-related genes.Genetic and molecular investigations highlight the antagonistic relationship between BnaC09.FUL and BnaC06.WIP2 in regulating the flowering time in B.napus through direct regulation of the expression of BnaC03.SOC1,BnaA08.CAL,and BnaC03.CAL.Overall,our findings provide a mechanism by which the BnaC09.FUL–BnaC06.WIP2 transcriptional regulatory module controls the flowering time in B.napus.展开更多
Photoperiod and temperature are crucial factors that trigger flowering in Brassica juncea(B.juncea).However,the underlying regulatory mechanisms remain poorly understood.The MADS-box transcription factor AGL18 acts as...Photoperiod and temperature are crucial factors that trigger flowering in Brassica juncea(B.juncea).However,the underlying regulatory mechanisms remain poorly understood.The MADS-box transcription factor AGL18 acts as a pivotal repressor of floral transition and functions redundantly with AGL15.In this study,we isolated BjuAGL18-1 from B.juncea and identified two unique transcripts,resulting in two distinct proteins:a full-length protein,BjuAGL18-1L,and a truncated protein,BjuAGL18-1S.Further investigation showed that the two isoforms had similar subcellular localizations but different expression patterns in various plant tissues.Notably,BjuAGL18-1L and BjuAGL18-1S were abundantly induced under short-and long-day photoperiods,respectively.BjuAGL18-1L overexpression in B.juncea and Arabidopsis thaliana(A.thaliana)led to late flowering,whereas BjuAGL18-1S overexpression resulted in early flowering.Yeast two-hybrid,bimolecular fluorescent complementation,and luciferase complementation assays showed that BjuAGL18-1L,but not BjuAGL18-1S(which lacked the EAR motif),interacted with the co-repressor BjuAFR2 and the histone deacetylase BjuHDA9 to form a multiprotein complex.Further analysis indicated that BjuAGL18-1L could also form a complex with BjuAGL15 and bind to the BjuFUL promoter,thus inhibiting its expression.However,BjuAGL18-1S interacted with BjuAGL18-1L to form heterodimers,which attenuated their activities,likely by disrupting their binding to target genes,resulting in accelerated flowering progression.These results suggest that BjuAGL18-1 is involved in photoperiod-induced flowering via different regulatory mechanisms in B.juncea.展开更多
Flowering time(or heading date)is a crucial agronomic trait for the adaptation of rice to specific growing regions and seasons.Although many flowering time-related rice genes have been identified and functionally char...Flowering time(or heading date)is a crucial agronomic trait for the adaptation of rice to specific growing regions and seasons.Although many flowering time-related rice genes have been identified and functionally characterized,continuing in-depth research is revealing how transcription of these genes is regulated.In this study,we determined that a basic leucine zipper transcription factor(OsbZIP40)and its homologous protein(OsbZIP12)participate in the control of flowering time.Overexpression of OsbZIP40 delayed flowering.Double mutants in which both OsbZIP40 and OsbZIP12 were knocked out exhibited an early-flowering phenotype under both long-day and short-day conditions.However,there was no difference in the heading date between the wild-type and each single mutant.These results suggest that OsbZIP40 functions as a flowering suppressor.Both OsbZIP40 and OsbZIP12 bound directly to the Ehd1 promoter and repressed its expression.Furthermore,MOTHER OF FT AND TFL1(Os MFT1)interacted with OsbZIP40/OsbZIP12 and enhanced their repressive effects on Ehd1 expression.Based on the data,we present a transcriptional regulatory mechanism in which OsbZIP40 and OsbZIP12 interact with Os MFT1 and modulate Ehd1 expression to delay flowering.Our findings provide relevant insights into the molecular mechanisms regulating flowering time in rice.展开更多
Foxtail millet(Setaria italica)is an important crop and an emerging model plant.Photoperiodic flowering is a key determinant of its production and geographic expansion.In this study,we found that SiPRR37 is responsibl...Foxtail millet(Setaria italica)is an important crop and an emerging model plant.Photoperiodic flowering is a key determinant of its production and geographic expansion.In this study,we found that SiPRR37 is responsible for the major quantitative trait locus(QTL)Heading date 2(Hd2)identified in 680 foxtail millets using a genome-wide association study.Overexpression of SiPRR37 in foxtail millet significantly delayed the heading date under both natural long-day and short-day conditions.CRISPR/Cas9-induced Siprr37 mutants exhibited earlier flowering in long-day conditions but later flowering in short-day conditions.The critical day length(CDL)for the reversal of Siprr37’s function was around 14.3 h.Haplotype analysis revealed that accessions with the Tc1-Mariner transposon insertion in SiPRR37(Hap 1)flowered significantly earlier at higher latitudes,and later at lower latitudes,indicating that natural variants of SiPRR37 exert dual functions in flowering regulation according to geographic latitude.The gradual,successive decrease in the frequency of Hap 2 from low to high latitudes,with the concurrent increase of Hap 1,demonstrates that these haplotypes have undergone artificial selection.Further FST analysis demonstrated that SiPRR37 has contributed to the ecological adaption of foxtail millet.Additionally,we reveal that OsPRR37 promotes flowering in rice,while GmPRR37 may only inhibit flowering in soybean.Further diurnal expression and transgenic analyses suggest that the dual function of SiPRR37 might depend on SiHd1.Our study uncovered the distinct functional reversal of SiPRR37 and functional diversification of PRR37 homologs in SD crops.These findings not only enrich knowledge about the regulation of photoperiodic flowering,but also contribute to genetic improvement of crops’regional adaptability.展开更多
The complex of calcineurin B-like protein(CBL)and CBL-interacting protein kinase(CIPK)serves as key components in calcium-sensing,orchestrating various signals crucial for plant growth,development,and responses to bio...The complex of calcineurin B-like protein(CBL)and CBL-interacting protein kinase(CIPK)serves as key components in calcium-sensing,orchestrating various signals crucial for plant growth,development,and responses to biotic and abiotic stresses.However,the mechanism underlying the response of this module to cold stress and its role in flower development in wintersweet(Chimonanthus praecox)remains unclear.Through expression pattern analysis,calcium ion(Ca^(2+))concentration assays,correlation analysis,and linear regression analysis,we found that the[Ca^(2+)],along with CpCBL8 and CpCIPK9 expression levels in wintersweet flower buds(FBs),significantly decreased during the initial flowering stage when the chilling requirement reached 570 chill units(CU).Notably,there was a significant positive correlation between[Ca^(2+)]and CpCBL8 expression.Ca^(2+)increased the expression of Cp CBL8 and CpCIPK9 in FBs,causing a significant delay in the flowering of wintersweet.Furthermore,the function of CpCBL8 was studied using heterologous transformation.Overexpression of CpCBL8 significantly delayed flowering time and significantly reduced cold tolerance and altered the expression pattern of endogenous genes related to low-temperature stress and flower development in transgenic Arabidopsis thaliana.Additionally,transcriptome analysis of chilling-induced dormancy breaking and flower bud enlargement revealed that CpCBL8 and CpCIPK9 were negatively regulated by cold,and the expression pattern of endogenous genes related to flower development and cold stress in wintersweet were similar to that of in A.thaliana.Moreover,protein-protein interaction(PPI)analysis revealed that CpCBL8 and CpCIPK9 interacted in the plasma membrane and nucleus.On the basis of these findings,we speculated that the CpCBL8-CpCIPK9 module plays a crucial role in regulating responses to cold stress and flower development in wintersweet.This study elucidated molecular mechanisms through which the downregulation of the Ca^(2+)-induced CpCBL8-CpCIPK9 module results in dormancy breaking and enhances cold tolerance.This study provides valuable insights for the cultivation of new varieties of wintersweet with increased ornamental value and enhanced cold stress tolerance.展开更多
A pot experiment was conducted to study the influences of foliar application of glycine,alanine,lysine,and glutamic acid in 200 mg/kg or 500 mg/kg upon the quality and enzyme activity of flowering Chinese cabbage（Bra...A pot experiment was conducted to study the influences of foliar application of glycine,alanine,lysine,and glutamic acid in 200 mg/kg or 500 mg/kg upon the quality and enzyme activity of flowering Chinese cabbage（Brassica parachinensis Bailey）.The results showed that all the application of these four amino acids could increase the yield of flowering Chinese cabbage,significantly raise the content of soluble sugar,and reduce the accumulation of nitrate.The applications of three other amino acids except alanine can increase the content of soluble proteins and decrease the accumulation of oxalic acid.However,the application of amino acid has insignificant influences on the SPAD number of chlorophyll,and causes the decrease of Vitamin C content.Meanwhile,the application of amino acid can improve the activity of nitrate reductase（NR） and glutamate dehydrogenase（GDH） as well.It shows that the application of amino acid is beneficial to improve ammonia metabolism,reduce the accumulation of nitrate and oxalic acid,increase the content of soluble sugar and soluble proteins,and improve the quality of flowering Chinese cabbage.展开更多
[Objective]The aim was to select the suitable hormone factors for flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.[Method]The test-tube plantlets from the stems of Dendrobium officinate Ki...[Objective]The aim was to select the suitable hormone factors for flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.[Method]The test-tube plantlets from the stems of Dendrobium officinate Kimura et Migo were used as the experimental materials and MS medium as the basic medium.Comparative tests have been done between single-factor hormone treatments（different concentrations of PP333 or TDZ） and multi-factor hormone treatments（different combinations of PP333,TDZ,6-BA and NAA） to research the effects of hormone factors on the flowering induction of the plantlets.[Result]Among the single-factor hormone treatments,the suitable concentration and the rate of flower buds formation of PP333 treatment were 0.2 mg/L and 8.5%,the that of TDZ treatment were 0.06 mg/L and 15.5%;the effects of multi-factor hormone treatments on the flowering induction were ordered as follow：（PP333 ＋ 6-BA ＋ NAA ＋ TDZ）〉 （PP333 ＋ 6-BA ＋ NAA）〉 （PP333 ＋ 6-BA） and（PP333 ＋ NAA） ;the most suitable treatment was PP333 0.3 mg/L ＋ 6-BA 0.5 mg/L ＋ NAA 0.5 mg/L ＋ TDZ 0.06 mg/L,the rate of flower bud formation and the rate of the blossomed flower were respectly reached to 80.4% and 90.3%.[Conclusion]PP333 and TDZ showed the important effect on the flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.The effect of TDZ was better than that of PP333.It is much more conducive to the flower bud formation,when using appropriate concentration of TDZ combined with other hormones properly.展开更多
[Objective] This study was to use reverse genic male sterile FHS better in field production. [Method] Low energy nitrogen ion beam was taken as a mutation source to conduct mutagenic treatment for indica rice 99-02 an...[Objective] This study was to use reverse genic male sterile FHS better in field production. [Method] Low energy nitrogen ion beam was taken as a mutation source to conduct mutagenic treatment for indica rice 99-02 and the mutant FHS with special fertility that was isolated from their offspring. Some characteristics such as flowering habit and stigma exsertion rate of FHS were observed in this experiment. [Result] The reverse genic sterile rice FHS had an obvious peak flowering stage from 10:00 to 10:30, while the second peak flowering was from August 5 to August 10. Compared with PA64S, FHS flowered early and its flowering time was concentrated, showing that it is for seed propagation. The stigma exsertion rate of FHS was 85.8% and low exsertion rate was good for the purity of seed. [Conclusion] The reverse genic sterile rice FHS had good value in use, besides, it could also be used as comparison material for studying fertility alternation mechanism of photoperiod sensitive genic male sterile rice.展开更多
Effects of two media and fertilizer levels on the growth of Cymbidium hybridium were studied. Results demonstrated that peanut\|hull mixed sand (abbreviated as PH/S=1/1) medium had higher total porosity and larger...Effects of two media and fertilizer levels on the growth of Cymbidium hybridium were studied. Results demonstrated that peanut\|hull mixed sand (abbreviated as PH/S=1/1) medium had higher total porosity and larger air space and lower water holding capacity in comparison with sphagnum (abbreviated as SP) medium. Leaf growth rate, chlorophyll contents, fresh weight and photosynthetic rate change along with the changes of nitrogen concentrations of both SP and PH/S media. According to the investigation,when the nitrogen concentration was 222 75?mg·L -1 , the photosynthetic rate of SP\|medium reached the highest. The practical nitrogen content was 180 00?mg·L -1 for PH/S medium . The maximum of flower quantity (number per spike) was gotten by treating of N/P 2O 5/K 2O=10/30/20 on PH/S medium, and N/P 2O 5/K 2O=0/23/29 treatment was the lowest on PH/S medium. The differences in small flowers, stem length and flower quantity among the two media and the three fertilization methods reached significant at 0 05 level,respectively.展开更多
基金supported by the National Key Research and Development Program of China(2022YFD1200400)the National Natural Science Foundation of China(32272111)+4 种基金Special fund for youth team of the Southwest Universities(SWU-XJPY202306)Chongqing Natural Science Foundation(CSTB2024NSCQLZX0012)Modern Agro-industry Technology Research System(CARS-12)Chongqing Modern Agricultural Industry Technology System(COMAITS202504)Biological Breeding-National Science and Technology Major Project(2022ZD04008).We sincerely appreciate the Plant Editors team for English language editing of the manuscript,which significantly improved its clarity and overall quality.
文摘Flowering time is a critical agronomic trait with a profound effect on the productivity and adaptabillity of rapeseed(Brassica napus L.).Strategically advancing flowering time can reduce the risk of yield losses due to extreme climatic conditions and facilitate the cultivation of subsequent crops on the same land,thereby enhancing overall agricultural efficiency.In this review,we synthesize current information on flowering time regulation in rapeseed through an integrated analysis of its genetic,hormonal,and environmental dimensions,emphasizing their crosstalk and implications for yield.We consolidate multi-omics evidence from population genetics,functional genomics,and systems biology to create a haplotype-based framework that overcomes the trade-off between flowering time and yield,providing support for the precision breeding of early-maturing cultivars.The insights presented here could inform future research on flowering time regulation and guide strategies for increasing rapeseed productivity.
基金supported by the National Natural Science Foundation of China(No.82373977,82173916)Wenzhou Major Science and Technology Innovation Projects(No.ZNF2023007)+1 种基金the“San Nong Jiu Fang”Science and Technology Cooperation Project of Zhejiang Province(No.2024SNJF039)Tongxiang Science and Technology Plan Project(No.202301017).
文摘Objective Anoectochilus roxburghii is a valuable medicinal and ornamental plant.The aim of this study is to investigate the morphological and biochemical responses during the flower development stages of A.roxburghii,and to assess the effects of exogenous polyamines(PAs)on bud differentiation and metabolism,thereby providing a theoretical basis for understanding the flowering form and physiology of A.roxburghii.Methods In this study,morphological and biochemical responses in flower development stages of A.roxburghii were investigated using paraffin sections and stereomicroscope.A.roxburghii was divided into five periods,including vegetative growth period,flower bud period,flowering period,late flowering period and fruiting period.During the flowering phase,specific biochemical parameters were measured,including soluble sugar content,superoxide dismutase(SOD)activity,soluble protein content,peroxidase(POD)activity,and catalase(CAT)activity.These measurements were conducted to understand the biochemical changes occurring within A.roxburghii during its flowering process.Furthermore,the effects of PAs on bud differentiation were examined.Additionally,the activities of S-adenosylmethionine decarboxylase(SAMDC)and polyamine oxidase(PAO),as well as the content of polyphenols,polysaccharides,and flavonoids in A.roxburghii,were measured after PA treatment to evaluate the metabolic changes induced by exogenous PAs.Results During the flowering phase of A.roxburghii,soluble sugar content and SOD activity were steadily declining.Soluble protein content was initially increasing and then reducing,and POD and CAT activities showed opposite pattern.In addition,the effects of exogenous PAs on bud differentiation were investigated.Results showed that 3 mmol/L putrescine or 0.3 mmol/L spermidine significantly promoted the bud differentiation of A.roxburghii and advanced the flowering.The activities of SAMDC,PAO,and the content of polyphenols,polysaccharides and flavonoids in A.roxburghii significantly increased after PA treatment,demonstrating that exogenous PA can accelerate metabolism and improved the active ingredients content.Conclusion The flower development of A.roxburghii was divided into five stages,with significant changes in soluble sugar,protein,POD,SOD,CAT,MDA,and PRO levels.Exogenous putrescine and spermidine enhanced bud differentiation and accelerated flowering,increasing SAMDC and PAO activities,suggesting accelerated PA metabolism.PAs also improved active component content.These findings provide a theoretical basis for studying flower morphology and PA-induced flowering regulation of A.roxburghii.
基金supported by the National Natural Science Foundation of China(32360743,32072559,and31860568)the National Key Research and Development Program,China(2018YFD1000800)the Fostering Foundation for the Excellent Ph D Dissertation of Gansu Agricultural University,China(YB2022004)。
文摘Flowering is one of the most important phenological periods,as it determines the timing of fruit maturation and seed dispersal.To date,both nitric oxide(NO)and DNA demethylation have been reported to regulate flowering in plants.However,there is no compelling experimental evidence for a relationship between NO and DNA demethylation during plant flowering.In this study,an NO donor and a DNA methylation inhibitor were used to investigate the involvement of DNA demethylation in NO-mediated tomato(Solanum lycopersicum cv.Micro-Tom)flowering.The results showed that the promoting effect of NO on tomato flowering was dose-dependent,with the greatest positive effect observed at 10μmol L^(-1) of the NO donor S-nitrosoglutathione(GSNO).Treatment with 50μmol L^(-1) of the DNA methylation inhibitor 5-azacitidine(5-AzaC)also significantly promoted tomato flowering.Moreover,GSNO and 5-AzaC increased the peroxidase(POD)and catalase(CAT)activities and cytokinin(CTK)and proline contents,while they reduced the gibberellic acid(GA3)and indole-3-acetic acid(IAA)contents.Co-treatment with GSNO and 5-AzaC accelerated the positive effects of GSNO and 5-AzaC in promoting tomato flowering.Meanwhile,compared with a GSNO or 5-AzaC treatment alone,co-treatment with GSNO+5-AzaC significantly increased the global DNA demethylation levels in different tissues of tomato.The results also indicate that DNA demethylation may be involved in NO-induced flowering.The expression of flowering genes was significantly altered by the GSNO+5-AzaC treatment.Five of these flowering induction genes,ARGONAUTE 4(AGO4A),SlSP3D/SINGLE FLOWER TRUSS(SFT),MutS HOMOLOG 1(MSH1),ZINC FINGER PROTEIN 2(ZFP2),and FLOWERING LOCUS D(FLD),were selected as candidate genes for further study.An McrBC-PCR analysis showed that DNA demethylation of the SFT gene in the apex and the FLD gene in the stem might be involved in NO-induced flowering.Therefore,this study shows that NO might promote tomato flowering by mediating the DNA demethylation of flowering induction genes,and it provides direct evidence for a synergistic effect of NO and DNA demethylation in promoting tomato flowering.
基金supported by the National Natural Science Foundation of China(no.32200306,32170385 and 32070362)the Postdoctoral Directional Training Foundation of Yunnan Province,and the Second Tibetan Plateau Scientific Expedition and Research(STEP)program(no.2019QZKK0502).
文摘Tibetan turnip and oilseeds are the most important vegetables cultivated in the Qinghai-Tibet Plateau.Our field observations identified a dominant early-bolting phenotype at the vegetative growth stage in the Tibetan turnip,which was possibly due to cross-pollination contamination from nearby oilseeds.We performed genetic and molecular experiments to explore the main reason for early bolting.We first analyzed gene expression and genomic sequence variation of turnip and oilseed BraFLC2,a gene that acts as a key repressor of flowering in turnip in a dosage-dependent manner.We found that the differences in flowering time and life habits between turnip and oilseeds were closely correlated with the genetic variations in BraFLC2.We further identified that the early-bolting turnip was the hybrid between turnip and oilseeds by selecting BraFLC2 as a testing gene.Furthermore,using an artificial hybridization experiment,we found that the heterozygote and low levels of BraFLC2 expression promoted early bolting in hybrid plants.These findings indicate that early-bolting in turnip is caused by cross-pollination contamination from oilseeds under human agricultural activities.We propose a strategy of strict seed screening,cultivation isolation and turnip breeding to ensure high quality and yield.
基金supported by Yunnan Province Agricultural Joint Key Project(Grant No.202401BD070001-016)the National Natural Science Foundation of China(Grant No.32202530)+3 种基金Talent Introduction and Training Project of Yunnan Academy of Agricultural Sciences(Grant No.2024RCYP-09)Fundamental Research Project(Grant No.202401CF070046)Xingdian Talent support program(XDYC-QNRC-2023-0457)Yunnan Technology Innovation Center of Flower Technique.
文摘The proper flowering time of rose(Rosa hybrida)is vital for the market value of this horticultural crop,but the mechanism regulating this trait is largely unclear.Here,we found that the transcription factor SQUAMOSA PROMOTER BINDING PROTEIN-LIKE4(RhSPL4)positively regulates flowering time in rose.Transient silencing or overexpression transgenic rose plants of RhSPL4 exhibited delayed or early flowering,respectively.Analysis of transcriptome data from transgenic lines overexpressing RhSPL4 compared to the wild type indicated that differentially expressed genes were significantly enriched in the circadian rhythm pathway.Among the proteins encoded by these genes,RhSPL4 binds to the promoter of PSEUDO-RESPONSE REGULATOR 5-LIKE(RhPRR5L),as revealed in yeast one-hybrid,dual-Luciferase/Renilla luciferase reporter,chromatin immunoprecipitation-quantitative PCR and electrophoretic mobility shift assay.Furthermore,RhSPL4 specifically binds to the478 to441 bp region of the RhPRR5L promoter and activates its transcription.The silencing of RhPRR5L delayed flowering time in rose,resembling the phenotype of RhSPL4-silenced plants.Together,these results indicate that the RhSPL4-RhPRR5L module positively regulates flowering time in rose,laying the foundation for the genetic improvement of flowering time in this important horticultural crop.
基金supported by grants from the National Natural Science Foundation of China(Grant No.31930100)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions。
文摘Nitrogen(N)is a key component in plants and their biological macromolecules,having a profound effect on developmental stages,such as germination,vegetative growth,and flowering.However,the mechanism of nitrogen-regulated flowering time remains unclear.In this study,CmNLP7 was isolated from the chrysanthemum cultivar‘Jinba'and was characterized.CmNLP7 is a transcription factor localized in the nucleus but has no transcriptional activity.Tissue expression pattern analysis showed that CmNLP7 was mainly transcribed in leaves and roots.Knocking down CmNLP7 through the artificial-miRNA method in chrysanthemum resulted in early flowering under optimal nitrogen(ON)and low nitrogen(LN)conditions;whereas overexpression lines showed delayed flowering under LN conditions.Transcriptome sequencing analysis showed that the nitrate transporters NRT2.5,NPF3.1,and NPF4.6;SBP-like genes SPL7 and SPL12,and flowering integration factor FT were significantly up-regulated in the knockdown lines.Based on the KEGG pathway enrichment analysis,the differentially transcribed genes were enriched in phenylpropanoid biosynthesis and starch and sucrose metabolism pathways,which indicated their alleged function in nitrogen-regulated flowering and development in chrysanthemum.Furthermore CmPP6 as a homolog of the Arabidopsis phosphatase PP6,was verified as an interacting protein of CmNLP7 by yeast two-hybrid,BiFC,pull-down and Biacore in vitro and in vivo,and the knockdown line of CmPP6(amiR-CmPP6)flowered earlier compared to that of the wild-type chrysanthemum‘Jinba'.Collectively,these results demonstrated that CmPP6 interacts with CmNLP7 to regulate chrysanthemum flowering,and CmNLP7 could regulate flowering time in response to nitrogen,which lays a foundation for the regulation of flowering and molecular breeding of chrysanthemum through changes in nutrient signaling.
文摘The peony has become a cultural and economic powerhouse in Heze.The peony,revered as the“king of flowers”in China,has long transcended its botanical identity to become a cultural icon deeply rooted in the Yellow River basin.In the fertile plains of the Yellow River,Heze City in Shandong Province has long held a special connection with the peony.
基金supported by grants from the National Key Research and Development Program of China(2023YFD120300)the National Natural Science Foundation of China(32201869)the Earmarked Fund for China Agriculture Research System(CARS-04)。
文摘In plants,numerous non-Mendelian inherited dominant effects,including over-,incomplete-,and codominance,are frequently observed,yet they remain insufficiently understood.A novel phenotype has been identified in specific soybean transformants overexpressing a single 35S::GmFT2a copy:superearly flowering dominance is exclusively observed in hemizygotes,not in homozygotes.Homozygous individual exhibits si RNA-mediated DNA methylation,causing epigenetic transcriptional silencing,whereas no such effect occurs in hemizygotes.Intriguingly,two distinct rounds of DNA methylation establishment occur,each mediated by a different mechanism.The homozygotes that derived from the hemizygous mother plants carrying 35S::GmFT2a locus was associated with the initiation of CHHcontext DNA methylation at 35S promoters mediated by 21 and 22 nucleotide(nt)si RNAs.Subsequently,24 nt si RNAs contribute to additional CHG-and CG-context DNA methylation at 35S promoters during the homozygosity of genes in plants already homozygous in maternal lineage.Reducing DNA methylation levels can be achieved by generating a hemizygous genotype through a crossing experiment with a recessive genotype.This research has unveiled a phenomenon:hemizygote-dependent dominance resulting from transcriptional silencing in homozygote offsprings.It provides new insights into the molecular mechanism underlying dominant effects.
基金supported by the National Natural Science Foundation of China(Grant No.32160142)the Guangxi Natural Science Foundation(Grant No.2023GXNSFDA026034)+3 种基金the Sugarcane Research Foundation of Guangxi University(Grant No.2022GZA002)the State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources(Grant No.SKLCUSAb202302)the National Natural Science Foundation of China(Grant No.31960051)the Natural Science Foundation for Young Scientists of Jiangxi Province(Grant No.20192ACB21005).
文摘Moso bamboo holds significant economic importance in China,serving various purposes,such as food,material,ornamentation,and greenery.Despite its versatility,the occurrence of flowering in Moso bamboo poses a threat to bamboo forests,resulting in substantial losses.The underlying cause of bamboo flowering remains elusive.Dynamic fluctuations in DNA methylation govern the transcriptional levels of crucial genes pivotal for plant growth and development.In this study,we conducted comprehensive DNA methylation(by whole-genome bisulfite sequencing)and transcriptome(by RNA-seq)analyses on non-flowering leaves,flowering leaves,and spikelets of Moso bamboo.Our findings revealed a notable reduction in the overall DNA methylation level,particularly CHH methylation,from leaves to spikelets,influencing the expression of differentially regulated genes.Notably,we identified DNA methylation as a regulatory mechanism for numerous flowering-related genes,including SPLs,FT,and SOC1.Specifically,the SPL3f gene,a key regulator of the aging pathway,exhibited hypomethylation and a high expression level in spikelets.Conversely,SOC1c displayed transcriptional silencing attributed to hypermethylation in the CHH context in the leaves of non-flowering plants.DNA methylation may affect the flowering mechanism of Moso bamboo by regulating the expression of key genes.In summary,our results shed light on the dynamic changes in DNA methylation between leaves and spikelets,unraveling an important epigenetic modification mechanism for flowering in Moso bamboo.
基金supported by the National Key Research and Development Program of China(2022YFD1200400)the Scientific and Technological Innovation Team of Shaanxi Province(2024RSCXTD-69)+1 种基金the Key Research and Development Program of Shaanxi Province(2021LLRH-07)a grant from the Yang Ling Seed Industry Innovation Center(K3031122024).
文摘Appropriate flowering time in rapeseed(Brassica napus L.)is vital for preventing losses from weather,diseases,and pests.However,the molecular basis of its regulation remains largely unknown.Here,a genome-wide association study identifies BnaC09.FUL,a MADS-box transcription factor,as a promising candidate gene regulating flowering time in B.napus.BnaC09.FUL expression increases sharply in B.napus shoot apices near bolting.BnaC09.FUL overexpression results in early flowering,while BnaFUL mutation causes delayed flowering in B.napus.A zinc finger transcription factor,BnaC06.WIP2,is identified as an interaction partner of BnaC09.FUL,and BnaC06.WIP2 overexpression delays flowering in B.napus,with RNA sequencing revealing its influence on the expression of many flowering-associated genes.We further demonstrate that BnaC06.WIP2 directly represses the expression of BnaA05.SOC1,BnaC03.SOC1,BnaC04.SOC1,BnaC06.FT,BnaA06.LFY,BnaC07.FUL,BnaA08.CAL,and BnaC03.CAL and indirectly inhibits the expression of other flowering time-related genes.Genetic and molecular investigations highlight the antagonistic relationship between BnaC09.FUL and BnaC06.WIP2 in regulating the flowering time in B.napus through direct regulation of the expression of BnaC03.SOC1,BnaA08.CAL,and BnaC03.CAL.Overall,our findings provide a mechanism by which the BnaC09.FUL–BnaC06.WIP2 transcriptional regulatory module controls the flowering time in B.napus.
基金supported by grants from the National Natural Science Foundation of China(32172547).
文摘Photoperiod and temperature are crucial factors that trigger flowering in Brassica juncea(B.juncea).However,the underlying regulatory mechanisms remain poorly understood.The MADS-box transcription factor AGL18 acts as a pivotal repressor of floral transition and functions redundantly with AGL15.In this study,we isolated BjuAGL18-1 from B.juncea and identified two unique transcripts,resulting in two distinct proteins:a full-length protein,BjuAGL18-1L,and a truncated protein,BjuAGL18-1S.Further investigation showed that the two isoforms had similar subcellular localizations but different expression patterns in various plant tissues.Notably,BjuAGL18-1L and BjuAGL18-1S were abundantly induced under short-and long-day photoperiods,respectively.BjuAGL18-1L overexpression in B.juncea and Arabidopsis thaliana(A.thaliana)led to late flowering,whereas BjuAGL18-1S overexpression resulted in early flowering.Yeast two-hybrid,bimolecular fluorescent complementation,and luciferase complementation assays showed that BjuAGL18-1L,but not BjuAGL18-1S(which lacked the EAR motif),interacted with the co-repressor BjuAFR2 and the histone deacetylase BjuHDA9 to form a multiprotein complex.Further analysis indicated that BjuAGL18-1L could also form a complex with BjuAGL15 and bind to the BjuFUL promoter,thus inhibiting its expression.However,BjuAGL18-1S interacted with BjuAGL18-1L to form heterodimers,which attenuated their activities,likely by disrupting their binding to target genes,resulting in accelerated flowering progression.These results suggest that BjuAGL18-1 is involved in photoperiod-induced flowering via different regulatory mechanisms in B.juncea.
基金supported by the National Key Research and Development Program of China(2024YFE0103400)the Natural Science Foundation of Jiangsu Province(BK20230013)+3 种基金the National Natural Science Foundation of China(32100259)the Program of Jiangsu Province Government(JBGS[2021]001-1-2)the Program of Zhongshan Biological Breeding Laboratory(ZSBBLKY2023-01)the PAPD Program from Jiangsu Government。
文摘Flowering time(or heading date)is a crucial agronomic trait for the adaptation of rice to specific growing regions and seasons.Although many flowering time-related rice genes have been identified and functionally characterized,continuing in-depth research is revealing how transcription of these genes is regulated.In this study,we determined that a basic leucine zipper transcription factor(OsbZIP40)and its homologous protein(OsbZIP12)participate in the control of flowering time.Overexpression of OsbZIP40 delayed flowering.Double mutants in which both OsbZIP40 and OsbZIP12 were knocked out exhibited an early-flowering phenotype under both long-day and short-day conditions.However,there was no difference in the heading date between the wild-type and each single mutant.These results suggest that OsbZIP40 functions as a flowering suppressor.Both OsbZIP40 and OsbZIP12 bound directly to the Ehd1 promoter and repressed its expression.Furthermore,MOTHER OF FT AND TFL1(Os MFT1)interacted with OsbZIP40/OsbZIP12 and enhanced their repressive effects on Ehd1 expression.Based on the data,we present a transcriptional regulatory mechanism in which OsbZIP40 and OsbZIP12 interact with Os MFT1 and modulate Ehd1 expression to delay flowering.Our findings provide relevant insights into the molecular mechanisms regulating flowering time in rice.
基金supported by the National Natural Science Foundation of China(32101759,32241042)the National Key Research and Development Program of China(2023YFD1200700 and 2023YFD1200704).
文摘Foxtail millet(Setaria italica)is an important crop and an emerging model plant.Photoperiodic flowering is a key determinant of its production and geographic expansion.In this study,we found that SiPRR37 is responsible for the major quantitative trait locus(QTL)Heading date 2(Hd2)identified in 680 foxtail millets using a genome-wide association study.Overexpression of SiPRR37 in foxtail millet significantly delayed the heading date under both natural long-day and short-day conditions.CRISPR/Cas9-induced Siprr37 mutants exhibited earlier flowering in long-day conditions but later flowering in short-day conditions.The critical day length(CDL)for the reversal of Siprr37’s function was around 14.3 h.Haplotype analysis revealed that accessions with the Tc1-Mariner transposon insertion in SiPRR37(Hap 1)flowered significantly earlier at higher latitudes,and later at lower latitudes,indicating that natural variants of SiPRR37 exert dual functions in flowering regulation according to geographic latitude.The gradual,successive decrease in the frequency of Hap 2 from low to high latitudes,with the concurrent increase of Hap 1,demonstrates that these haplotypes have undergone artificial selection.Further FST analysis demonstrated that SiPRR37 has contributed to the ecological adaption of foxtail millet.Additionally,we reveal that OsPRR37 promotes flowering in rice,while GmPRR37 may only inhibit flowering in soybean.Further diurnal expression and transgenic analyses suggest that the dual function of SiPRR37 might depend on SiHd1.Our study uncovered the distinct functional reversal of SiPRR37 and functional diversification of PRR37 homologs in SD crops.These findings not only enrich knowledge about the regulation of photoperiodic flowering,but also contribute to genetic improvement of crops’regional adaptability.
基金funded by the Natural Science Foundation of Chongqing(CSTB2023NSCQ-MSX0236)Fundamental Research Funds for the Central Universities(SWU-XDJH202308)Earmarked Funds for the China Agriculture Research System(CARS-26)。
文摘The complex of calcineurin B-like protein(CBL)and CBL-interacting protein kinase(CIPK)serves as key components in calcium-sensing,orchestrating various signals crucial for plant growth,development,and responses to biotic and abiotic stresses.However,the mechanism underlying the response of this module to cold stress and its role in flower development in wintersweet(Chimonanthus praecox)remains unclear.Through expression pattern analysis,calcium ion(Ca^(2+))concentration assays,correlation analysis,and linear regression analysis,we found that the[Ca^(2+)],along with CpCBL8 and CpCIPK9 expression levels in wintersweet flower buds(FBs),significantly decreased during the initial flowering stage when the chilling requirement reached 570 chill units(CU).Notably,there was a significant positive correlation between[Ca^(2+)]and CpCBL8 expression.Ca^(2+)increased the expression of Cp CBL8 and CpCIPK9 in FBs,causing a significant delay in the flowering of wintersweet.Furthermore,the function of CpCBL8 was studied using heterologous transformation.Overexpression of CpCBL8 significantly delayed flowering time and significantly reduced cold tolerance and altered the expression pattern of endogenous genes related to low-temperature stress and flower development in transgenic Arabidopsis thaliana.Additionally,transcriptome analysis of chilling-induced dormancy breaking and flower bud enlargement revealed that CpCBL8 and CpCIPK9 were negatively regulated by cold,and the expression pattern of endogenous genes related to flower development and cold stress in wintersweet were similar to that of in A.thaliana.Moreover,protein-protein interaction(PPI)analysis revealed that CpCBL8 and CpCIPK9 interacted in the plasma membrane and nucleus.On the basis of these findings,we speculated that the CpCBL8-CpCIPK9 module plays a crucial role in regulating responses to cold stress and flower development in wintersweet.This study elucidated molecular mechanisms through which the downregulation of the Ca^(2+)-induced CpCBL8-CpCIPK9 module results in dormancy breaking and enhances cold tolerance.This study provides valuable insights for the cultivation of new varieties of wintersweet with increased ornamental value and enhanced cold stress tolerance.
基金Supported by National Scientific and Technological Supporting Project(2008BADA4B04-09)Guangdong Province Scientific and Technological Project(2008A020100017)Guangdong Province Department of Finance Project[(2006)143]~~
文摘A pot experiment was conducted to study the influences of foliar application of glycine,alanine,lysine,and glutamic acid in 200 mg/kg or 500 mg/kg upon the quality and enzyme activity of flowering Chinese cabbage（Brassica parachinensis Bailey）.The results showed that all the application of these four amino acids could increase the yield of flowering Chinese cabbage,significantly raise the content of soluble sugar,and reduce the accumulation of nitrate.The applications of three other amino acids except alanine can increase the content of soluble proteins and decrease the accumulation of oxalic acid.However,the application of amino acid has insignificant influences on the SPAD number of chlorophyll,and causes the decrease of Vitamin C content.Meanwhile,the application of amino acid can improve the activity of nitrate reductase（NR） and glutamate dehydrogenase（GDH） as well.It shows that the application of amino acid is beneficial to improve ammonia metabolism,reduce the accumulation of nitrate and oxalic acid,increase the content of soluble sugar and soluble proteins,and improve the quality of flowering Chinese cabbage.
基金Supported by Plan Project of Science and Technology Committee of Guangxi Province (0322024-3A) Scientific Research Foundation for Returned Scholars of Guangxi Province~~
文摘[Objective]The aim was to select the suitable hormone factors for flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.[Method]The test-tube plantlets from the stems of Dendrobium officinate Kimura et Migo were used as the experimental materials and MS medium as the basic medium.Comparative tests have been done between single-factor hormone treatments（different concentrations of PP333 or TDZ） and multi-factor hormone treatments（different combinations of PP333,TDZ,6-BA and NAA） to research the effects of hormone factors on the flowering induction of the plantlets.[Result]Among the single-factor hormone treatments,the suitable concentration and the rate of flower buds formation of PP333 treatment were 0.2 mg/L and 8.5%,the that of TDZ treatment were 0.06 mg/L and 15.5%;the effects of multi-factor hormone treatments on the flowering induction were ordered as follow：（PP333 ＋ 6-BA ＋ NAA ＋ TDZ）〉 （PP333 ＋ 6-BA ＋ NAA）〉 （PP333 ＋ 6-BA） and（PP333 ＋ NAA） ;the most suitable treatment was PP333 0.3 mg/L ＋ 6-BA 0.5 mg/L ＋ NAA 0.5 mg/L ＋ TDZ 0.06 mg/L,the rate of flower bud formation and the rate of the blossomed flower were respectly reached to 80.4% and 90.3%.[Conclusion]PP333 and TDZ showed the important effect on the flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.The effect of TDZ was better than that of PP333.It is much more conducive to the flower bud formation,when using appropriate concentration of TDZ combined with other hormones properly.
文摘[Objective] This study was to use reverse genic male sterile FHS better in field production. [Method] Low energy nitrogen ion beam was taken as a mutation source to conduct mutagenic treatment for indica rice 99-02 and the mutant FHS with special fertility that was isolated from their offspring. Some characteristics such as flowering habit and stigma exsertion rate of FHS were observed in this experiment. [Result] The reverse genic sterile rice FHS had an obvious peak flowering stage from 10:00 to 10:30, while the second peak flowering was from August 5 to August 10. Compared with PA64S, FHS flowered early and its flowering time was concentrated, showing that it is for seed propagation. The stigma exsertion rate of FHS was 85.8% and low exsertion rate was good for the purity of seed. [Conclusion] The reverse genic sterile rice FHS had good value in use, besides, it could also be used as comparison material for studying fertility alternation mechanism of photoperiod sensitive genic male sterile rice.
文摘Effects of two media and fertilizer levels on the growth of Cymbidium hybridium were studied. Results demonstrated that peanut\|hull mixed sand (abbreviated as PH/S=1/1) medium had higher total porosity and larger air space and lower water holding capacity in comparison with sphagnum (abbreviated as SP) medium. Leaf growth rate, chlorophyll contents, fresh weight and photosynthetic rate change along with the changes of nitrogen concentrations of both SP and PH/S media. According to the investigation,when the nitrogen concentration was 222 75?mg·L -1 , the photosynthetic rate of SP\|medium reached the highest. The practical nitrogen content was 180 00?mg·L -1 for PH/S medium . The maximum of flower quantity (number per spike) was gotten by treating of N/P 2O 5/K 2O=10/30/20 on PH/S medium, and N/P 2O 5/K 2O=0/23/29 treatment was the lowest on PH/S medium. The differences in small flowers, stem length and flower quantity among the two media and the three fertilization methods reached significant at 0 05 level,respectively.
