The purpose of present work is a measurement of telomere length dynamic in proliferating cells in vitro by modified flow-FISH method. This method is a combination of two modifications： telomere length measurement in ...The purpose of present work is a measurement of telomere length dynamic in proliferating cells in vitro by modified flow-FISH method. This method is a combination of two modifications： telomere length measurement in differentiated cells by surface antigen and analysis of cells divisions＇ number by vital dye dilution. Lymphocytes were activated by anti-CD3 Abs with IL-2 presents and grown in vitro for 7 days. Cells division＇s number was measured by dilution of CFSE vital dye which cells were stained previously activation. For telomere length measurement we used flow-FISH method with Cy3 labeled telomere PNH probe. Using this method we evaluated the dynamic of telomere length in CD4＋ and CD8＋ T-cells after 7 days culturing in vitro and revealed the difference in telomere lengthening and shortening versus division rounds in cell subsets. In CD8＋ cells telomeres start lengthen on a second division with the maximum on 4th division round becoming more that 20% longer compared with undividing cells. In CD4＋ cells telomeres did not have any length peculiarities through all division rounds demonstrating different telomere regulation in subsets. This probably occurs due to the higher level ofhTERT protein expression in CD8＋ than CD4＋ cells do.展开更多
W型潜坝作为一种新型的堰坝结构,不仅具有消能、护岸、分流和改善通航条件等功能,而且具有显著的生态功能。选取鲫鱼幼鱼为目标鱼类,研究不同粒径组成的W型潜坝的河流流速分布及对目标鱼类聚集的影响,探明W型潜坝的生境改良效果,为裁弯...W型潜坝作为一种新型的堰坝结构,不仅具有消能、护岸、分流和改善通航条件等功能,而且具有显著的生态功能。选取鲫鱼幼鱼为目标鱼类,研究不同粒径组成的W型潜坝的河流流速分布及对目标鱼类聚集的影响,探明W型潜坝的生境改良效果,为裁弯河段生态涵养区建设提供依据。研究结果表明不同粒径的W型潜坝作用下,[10,20]mm较大粒径组成的W型潜坝透水性更好,流速梯度小于小粒径组,流态多样性高。在流量0.09 m 3/s条件下,[10,20]mm粒径组成的W型潜坝鱼类聚集区域停留最多达到85次;[10,20]mm较大粒径组成的W型潜坝生境多样性高,可以为试验鱼提供更好的栖息和庇护场所。因此,在人工运河修建中裁弯河段生态涵养区建议选透水效果较好的大粒径潜坝方案。流态多样性指数与鱼类平均聚集度存在线性关系,可作为评价栖息地质量的依据,研究成果为河流生境异质性研究和生态优化设计提供理论支撑。展开更多
文摘The purpose of present work is a measurement of telomere length dynamic in proliferating cells in vitro by modified flow-FISH method. This method is a combination of two modifications： telomere length measurement in differentiated cells by surface antigen and analysis of cells divisions＇ number by vital dye dilution. Lymphocytes were activated by anti-CD3 Abs with IL-2 presents and grown in vitro for 7 days. Cells division＇s number was measured by dilution of CFSE vital dye which cells were stained previously activation. For telomere length measurement we used flow-FISH method with Cy3 labeled telomere PNH probe. Using this method we evaluated the dynamic of telomere length in CD4＋ and CD8＋ T-cells after 7 days culturing in vitro and revealed the difference in telomere lengthening and shortening versus division rounds in cell subsets. In CD8＋ cells telomeres start lengthen on a second division with the maximum on 4th division round becoming more that 20% longer compared with undividing cells. In CD4＋ cells telomeres did not have any length peculiarities through all division rounds demonstrating different telomere regulation in subsets. This probably occurs due to the higher level ofhTERT protein expression in CD8＋ than CD4＋ cells do.
文摘W型潜坝作为一种新型的堰坝结构,不仅具有消能、护岸、分流和改善通航条件等功能,而且具有显著的生态功能。选取鲫鱼幼鱼为目标鱼类,研究不同粒径组成的W型潜坝的河流流速分布及对目标鱼类聚集的影响,探明W型潜坝的生境改良效果,为裁弯河段生态涵养区建设提供依据。研究结果表明不同粒径的W型潜坝作用下,[10,20]mm较大粒径组成的W型潜坝透水性更好,流速梯度小于小粒径组,流态多样性高。在流量0.09 m 3/s条件下,[10,20]mm粒径组成的W型潜坝鱼类聚集区域停留最多达到85次;[10,20]mm较大粒径组成的W型潜坝生境多样性高,可以为试验鱼提供更好的栖息和庇护场所。因此,在人工运河修建中裁弯河段生态涵养区建议选透水效果较好的大粒径潜坝方案。流态多样性指数与鱼类平均聚集度存在线性关系,可作为评价栖息地质量的依据,研究成果为河流生境异质性研究和生态优化设计提供理论支撑。
