The progress of modern industry has given rise to great requirements for network transmission latency and reliability in domains such as smart grid and intelligent driving.To address these challenges,the concept of Ti...The progress of modern industry has given rise to great requirements for network transmission latency and reliability in domains such as smart grid and intelligent driving.To address these challenges,the concept of Time-sensitive networking(TSN)is proposed by IEEE 802.1TSN working group.In order to achieve low latency,Cyclic queuing and forwarding(CQF)mechanism is introduced to schedule Timetriggered(TT)flows.In this paper,we construct a TSN model based on CQF and formulate the flow scheduling problem as an optimization problem aimed at maximizing the success rate of flow scheduling.The problem is tackled by a novel algorithm that makes full use of the characteristics and the relationship between the flows.Firstly,by K-means algorithm,the flows are initially partitioned into subsets based on their correlations.Subsequently,the flows within each subset are sorted by a new special criteria extracted from multiple features of flow.Finally,a flow offset selecting method based on load balance is used for resource mapping,so as to complete the process of flow scheduling.Experimental results demonstrate that the proposed algorithm exhibits significant advantages in terms of scheduling success rate and time efficiency.展开更多
Objective: In this paper we compared the two methods of cell sorting (magnetic cell sorting and flow cytometry sorting) for the isolation and function analysis of mouse CD4+ CD25+ regulatory T (Treg) cells, in order t...Objective: In this paper we compared the two methods of cell sorting (magnetic cell sorting and flow cytometry sorting) for the isolation and function analysis of mouse CD4+ CD25+ regulatory T (Treg) cells, in order to inform further studies in Treg cell function. Methods: We separately used magnetic cell sorting and flow cytometry sorting to identify CD4+ CD25+ Treg cells. After magnetic cell separation, we further used flow cytometry to analyze the purity of CD4+ CD25+ Treg cells, trypan blue staining to detect cell viability, and propidium iodide (PI) staining to assess the cell viability. We detected the immune inhibition of CD4+ CD25+ Treg cells in the in vitro proliferation experiments. Results: The results showed that compared to flow cytometry sorting, magnetic cell sorting took more time and effort, but fewer live cells were obtained than with flow cytometry sorting. The CD4+ CD25+ Treg cells, however, obtained with both methods have similar immunosuppressive capacities. Conclusion: The result suggests that both methods can be used in isolating CD4+ CD25+ Treg cells, and one can select the best method according to specific needs and availability of the methodologies.展开更多
Wheat sharp eyespot, a stem disease caused by the soilborne fungus Rhizoctonia cerealis van der Hoeven,has become a threat to wheat production worldwide. Exploiting resistance resources from wild relatives of wheat is...Wheat sharp eyespot, a stem disease caused by the soilborne fungus Rhizoctonia cerealis van der Hoeven,has become a threat to wheat production worldwide. Exploiting resistance resources from wild relatives of wheat is a promising strategy for controlling this disease. In this study, a new wheat–Dasypyrum villosum T2DS·2V#4L translocation line in the background of Chinese Spring(CS) showed stable resistance to R. cerealis. Introgression of the T2DS·2V#4L chromosome into wheat cultivar Aikang 58 by backcrossing produced a marked increase in sharp eyespot resistance in NIL-T2DS·2V#4L in comparison with NILT2DS·2DL, and no detrimental effects of 2V#4L on agronomic traits were observed in the BC2F2, BC2F2:3,and BC2F2:4generations. Flow-sorted sequencing of 2V#4L yielded 384.3 Mb of assembled sequence, and8836 genes were predicted of which 6154 had orthologs in at least one of the 2AL, 2BL, and 2DL arms of CS, whereas 1549 genes were unique to 2V#4L. About 100,000 SNPs were detected in genes of 2V#4L and2DL in 10 sequenced bread wheat cultivars. A Kompetitive Allele Specific Polymerase chain reaction and30 conserved ortholog sequence markers were developed to trace the 2V#4L chromatin in wheat backgrounds. T2DS·2V#4L compensating translocation lines represent novel germplasm with sharp eyespot resistance and the markers will allow rapid detection in breeding programs.展开更多
基金supported by Science and Technology Project of State Grid Corporation Headquarters under Grant 5108-202218280A-2-170-XG(Development and Application of Power Time-Sensitive Network Switching Chip。
文摘The progress of modern industry has given rise to great requirements for network transmission latency and reliability in domains such as smart grid and intelligent driving.To address these challenges,the concept of Time-sensitive networking(TSN)is proposed by IEEE 802.1TSN working group.In order to achieve low latency,Cyclic queuing and forwarding(CQF)mechanism is introduced to schedule Timetriggered(TT)flows.In this paper,we construct a TSN model based on CQF and formulate the flow scheduling problem as an optimization problem aimed at maximizing the success rate of flow scheduling.The problem is tackled by a novel algorithm that makes full use of the characteristics and the relationship between the flows.Firstly,by K-means algorithm,the flows are initially partitioned into subsets based on their correlations.Subsequently,the flows within each subset are sorted by a new special criteria extracted from multiple features of flow.Finally,a flow offset selecting method based on load balance is used for resource mapping,so as to complete the process of flow scheduling.Experimental results demonstrate that the proposed algorithm exhibits significant advantages in terms of scheduling success rate and time efficiency.
基金Project supported by the National Natural Science Foundation of China (Nos. 30872578 and 30753761)the Natural Science Founda-tion of Shanxi Province (No. SJ08C201)+1 种基金the Science and Technology Key Projects Foundation of Shanxi Province (No. 2008K13-04)the Science and Technology Plan Projects Foundation of Xi’an (No. SF08006-2), China
文摘Objective: In this paper we compared the two methods of cell sorting (magnetic cell sorting and flow cytometry sorting) for the isolation and function analysis of mouse CD4+ CD25+ regulatory T (Treg) cells, in order to inform further studies in Treg cell function. Methods: We separately used magnetic cell sorting and flow cytometry sorting to identify CD4+ CD25+ Treg cells. After magnetic cell separation, we further used flow cytometry to analyze the purity of CD4+ CD25+ Treg cells, trypan blue staining to detect cell viability, and propidium iodide (PI) staining to assess the cell viability. We detected the immune inhibition of CD4+ CD25+ Treg cells in the in vitro proliferation experiments. Results: The results showed that compared to flow cytometry sorting, magnetic cell sorting took more time and effort, but fewer live cells were obtained than with flow cytometry sorting. The CD4+ CD25+ Treg cells, however, obtained with both methods have similar immunosuppressive capacities. Conclusion: The result suggests that both methods can be used in isolating CD4+ CD25+ Treg cells, and one can select the best method according to specific needs and availability of the methodologies.
基金supported by the National Key Research and Develpment Program of China (2021YFD1200600)the National Natural Science Foundation of China (31871619, 32101703, and 32101800)+4 种基金the Natural Science Foundation of Jiangsu Province (BK20210152)the Jiangsu Seed Industry Revitalization Project (JBGS (2021) 013)the Key Research and Development Program of Jiangsu Province(BE2022346)Jiangsu Agricultural Science and Technology Innovation Fund of China (CX (20) 3029)supported by the European Regional Development Fund (CZ.02.1.01/0.0/0.0/16_019/0000827)。
文摘Wheat sharp eyespot, a stem disease caused by the soilborne fungus Rhizoctonia cerealis van der Hoeven,has become a threat to wheat production worldwide. Exploiting resistance resources from wild relatives of wheat is a promising strategy for controlling this disease. In this study, a new wheat–Dasypyrum villosum T2DS·2V#4L translocation line in the background of Chinese Spring(CS) showed stable resistance to R. cerealis. Introgression of the T2DS·2V#4L chromosome into wheat cultivar Aikang 58 by backcrossing produced a marked increase in sharp eyespot resistance in NIL-T2DS·2V#4L in comparison with NILT2DS·2DL, and no detrimental effects of 2V#4L on agronomic traits were observed in the BC2F2, BC2F2:3,and BC2F2:4generations. Flow-sorted sequencing of 2V#4L yielded 384.3 Mb of assembled sequence, and8836 genes were predicted of which 6154 had orthologs in at least one of the 2AL, 2BL, and 2DL arms of CS, whereas 1549 genes were unique to 2V#4L. About 100,000 SNPs were detected in genes of 2V#4L and2DL in 10 sequenced bread wheat cultivars. A Kompetitive Allele Specific Polymerase chain reaction and30 conserved ortholog sequence markers were developed to trace the 2V#4L chromatin in wheat backgrounds. T2DS·2V#4L compensating translocation lines represent novel germplasm with sharp eyespot resistance and the markers will allow rapid detection in breeding programs.
