Objective:To investigate the osteogenic effects of polyphenol-rich extracts from Wisteria floribunda(Willd.)DC.(W.floribunda)flowers and elucidate the underlying mechanisms.Methods:Polyphenolic compounds of W.floribun...Objective:To investigate the osteogenic effects of polyphenol-rich extracts from Wisteria floribunda(Willd.)DC.(W.floribunda)flowers and elucidate the underlying mechanisms.Methods:Polyphenolic compounds of W.floribunda extracts were analyzed,including flavonoids and glucoside derivatives.Osteogenic activity was assessed in MC3T3-E1 preosteoblast cells by measurement of alkaline phosphatase(ALP)activity,alizarin red S staining,and the expression of osteogenic markers(RUNX2,SP7,and ALPL).In vivo effects were evaluated in zebrafish larvae by assessing skeletal development and expression of osteogenic genes(runx2a,sp7,and alpl).The role of mammalian target of rapamycin(mTOR)pathway was examined using rapamycin.Results:W.floribunda extracts significantly enhanced ALP activity,bone mineralization,and the expression of RUNX2,SP7,and ALPL in MC3T3-E1 cells.In zebrafish larvae,W.floribunda extracts improved vertebral mineralization and upregulated osteogenic genes.Mechanistically,the plant extract activated the mTOR pathway,and rapamycin treatment attenuated the extracts-induced ALP activity,mineralization,and vertebral formation in zebrafish,confirming mTOR involvement.Conclusions:W.floribunda extracts promote osteoblast differentiation and bone formation via mTOR pathway activation.These findings provide novel insights into the potential of W.floribunda extracts and support its further investigation as a natural therapeutic candidate for bone degenerative disorders such as osteoporosis.展开更多
A new naphthoquinone-anthraquinone dimer named floribundiquinone E （1） and a new anthraquinone 2-acetylphyscion （2） were isolated from the roots of Berchemia floribunda Brongn. Their structures were elucidated on ...A new naphthoquinone-anthraquinone dimer named floribundiquinone E （1） and a new anthraquinone 2-acetylphyscion （2） were isolated from the roots of Berchemia floribunda Brongn. Their structures were elucidated on the basis of spectroscopic methods.展开更多
Objective:Alchornea floribunda leaves are widely used in ethnomedicinal management of inflammatory disorders. The present work is aimed at investigating this folkloric use.Methods:The anti-inflammatory effect of the l...Objective:Alchornea floribunda leaves are widely used in ethnomedicinal management of inflammatory disorders. The present work is aimed at investigating this folkloric use.Methods:The anti-inflammatory effect of the leaf extracts and fractions was investigated in experimental animal models of acute and chronic inflammation. The possible mechanisms by which the two most active fractions,hexane(HE) and ethyl acetate(EF) exert their effects were also investigated.Results:The crude extract(200 mg/kg) showed moderate inhibition of egg albumen-induced edema in rats(%edema inhibition = 54.69) at 4 h.HE and EF showed very high activity(%edema inhibition of 81.25 and 67.19 respectively at 200 mg/kg) at 4h as compared to the control. Both fractions ameliorated arthritis induced by formaldehyde in rats.At 400 mg/kg,HE evofeed a significant irritation of gastric mucosa in rats.EF(200 mg/kg,p.o.) significantly inhibited leucocytes(%inhibition = 36.79) migration in vivo,but could not stabilize heat and hypotonicity-indueed lysis of human erythrocyte at 200 and 400μg/mL in vitro.Phytochemical investigation revealed the presence of terpenoids and steroids in HE and flavonoids,tannins and saponins in EF.Conclusion:These results suggest that the leaves of Alchornea floribunda possess anti-inflammatory activity in acute and chronic inflammation.The activity may derive from a combination of inhibition of prostaglandin synthesis and leucocytes migration.The phytochemical constituents detected in HE and EF may account for the anti-inflammatory activity.展开更多
Friable callus was induced when immature seeds of floribunda rose were inoculated on MS medium supplemented with 2,4-D 3.0 mg.L^-1. When transfered onto subculture media, friable callus developed into embryogenic call...Friable callus was induced when immature seeds of floribunda rose were inoculated on MS medium supplemented with 2,4-D 3.0 mg.L^-1. When transfered onto subculture media, friable callus developed into embryogenic callus, which was used to establish cell suspension lines. Cell suspensions had to be subcultured at a interval of 4-5 days at the first several culture cycles. The best subculturing cycle for the stable cell suspensions was 8-10 days. The best inoculum quantity was 1 mL PCV（Packed Cell Volume） per 40 mL culture fluid.展开更多
[Objective]This study aimed to screen out floribunda rose cultivars resistant to high temperature in tropical areas.[Method]A total of 8 floribunda rose cul-tivars(Carefree Wonder,Scloss Mannheim,Bright Smile,Goldmari...[Objective]This study aimed to screen out floribunda rose cultivars resistant to high temperature in tropical areas.[Method]A total of 8 floribunda rose cul-tivars(Carefree Wonder,Scloss Mannheim,Bright Smile,Goldmarie,Betty Prior,Red Riding Hood Fairy Tale,Golden Scepter and Iceberg)were selected as the research objects,and their growth,adaptability and disease resistance in Sanya area were compared.[Result]Carefree Wonder,Scloss Mannheim,Iceberg and Bright Smile were suitable to be planted in Sanya area.Among them,Carefree Wonder,Scloss Mannheim and Iceberg were characterized with fewer diseases,bright colors,heat resistance and strong stress resistance,and they could be planted as ornamental flowers in Sanya area.[Conclusion]This study will provide a reference for the selection of ornamental flowers in Sanya area.展开更多
Bredemeyera floribunda Wild (Polygalaceae), also known as pau-de-ovo is a medicinal plant used to treat snakebites, as tonic and fortifiers to treat fatigue and exhaustion. The aim of the present study was to document...Bredemeyera floribunda Wild (Polygalaceae), also known as pau-de-ovo is a medicinal plant used to treat snakebites, as tonic and fortifiers to treat fatigue and exhaustion. The aim of the present study was to document the knowledge of herbal sellers in the preparation of the so-called “B. floribunda fortifier” and to compare the plant root content with the recommended nutritional values and the permitted limit for medicinal plants. The study revealed that, unlike other regions of Brazil, in the Midwest region, the root of the plant is used as food to treat fatigue and exhaustion. The contents in B. floribunda root decreased in the following order: Ca > K > P > Na > Fe > Mg > Si > Mn > Zn > Cu. Daily intake of 30 grams of B. floribunda roots are considered as an excellent source of Cr, Cu, Mn and Se for people 19 to 50 years of age according to the RDA. In addition, Cu and Fe showed content above the established limit for medicinal plants stipulated by other countries. The root of the plant has low K, Mg, P, Na and Zn content. When the contents in roots of the plant are below the oral dose of reference, consumption of this plant can be considered safe and without risk to human health. On the other hand, concentration of elements such as Cu, Fe, Ni, Al, Cr and Se exceeding the allowable limit may cause serious health problems due to prolonged ingestion and large amounts consumed daily. The Brazilian legislation does not establish a permissible limit of macro- and micro-elements in medicinal plants;thus, the results presented in this study will contribute to the Brazilian public health policy on medicinal plants.展开更多
Germination of Lespedeza floribunda Bunge.seeds was studied in order to provide cultivation and application in gardening and landscaping.Results indicated that the seeds of Lespedeza floribunda had the characteristic ...Germination of Lespedeza floribunda Bunge.seeds was studied in order to provide cultivation and application in gardening and landscaping.Results indicated that the seeds of Lespedeza floribunda had the characteristic of forcing dormancy that was caused by hard seed vessels.The dormancy could be released by soaking in hot water and by sulphuric acid treatment.The most appropriate treatment time was 10～30 min,resulting in average germination rate(96%),average germination power(82.53%)and average germination index(17.54).The optimum germination temperature was 25 ℃ and the germination rate,germination power and germination index was 95%,81%,22.95 respectively after treated by sulphuric acid for 20 min.展开更多
基金supported by the 2024 scientific promotion program funded by Jeju National University.
文摘Objective:To investigate the osteogenic effects of polyphenol-rich extracts from Wisteria floribunda(Willd.)DC.(W.floribunda)flowers and elucidate the underlying mechanisms.Methods:Polyphenolic compounds of W.floribunda extracts were analyzed,including flavonoids and glucoside derivatives.Osteogenic activity was assessed in MC3T3-E1 preosteoblast cells by measurement of alkaline phosphatase(ALP)activity,alizarin red S staining,and the expression of osteogenic markers(RUNX2,SP7,and ALPL).In vivo effects were evaluated in zebrafish larvae by assessing skeletal development and expression of osteogenic genes(runx2a,sp7,and alpl).The role of mammalian target of rapamycin(mTOR)pathway was examined using rapamycin.Results:W.floribunda extracts significantly enhanced ALP activity,bone mineralization,and the expression of RUNX2,SP7,and ALPL in MC3T3-E1 cells.In zebrafish larvae,W.floribunda extracts improved vertebral mineralization and upregulated osteogenic genes.Mechanistically,the plant extract activated the mTOR pathway,and rapamycin treatment attenuated the extracts-induced ALP activity,mineralization,and vertebral formation in zebrafish,confirming mTOR involvement.Conclusions:W.floribunda extracts promote osteoblast differentiation and bone formation via mTOR pathway activation.These findings provide novel insights into the potential of W.floribunda extracts and support its further investigation as a natural therapeutic candidate for bone degenerative disorders such as osteoporosis.
文摘A new naphthoquinone-anthraquinone dimer named floribundiquinone E （1） and a new anthraquinone 2-acetylphyscion （2） were isolated from the roots of Berchemia floribunda Brongn. Their structures were elucidated on the basis of spectroscopic methods.
文摘Objective:Alchornea floribunda leaves are widely used in ethnomedicinal management of inflammatory disorders. The present work is aimed at investigating this folkloric use.Methods:The anti-inflammatory effect of the leaf extracts and fractions was investigated in experimental animal models of acute and chronic inflammation. The possible mechanisms by which the two most active fractions,hexane(HE) and ethyl acetate(EF) exert their effects were also investigated.Results:The crude extract(200 mg/kg) showed moderate inhibition of egg albumen-induced edema in rats(%edema inhibition = 54.69) at 4 h.HE and EF showed very high activity(%edema inhibition of 81.25 and 67.19 respectively at 200 mg/kg) at 4h as compared to the control. Both fractions ameliorated arthritis induced by formaldehyde in rats.At 400 mg/kg,HE evofeed a significant irritation of gastric mucosa in rats.EF(200 mg/kg,p.o.) significantly inhibited leucocytes(%inhibition = 36.79) migration in vivo,but could not stabilize heat and hypotonicity-indueed lysis of human erythrocyte at 200 and 400μg/mL in vitro.Phytochemical investigation revealed the presence of terpenoids and steroids in HE and flavonoids,tannins and saponins in EF.Conclusion:These results suggest that the leaves of Alchornea floribunda possess anti-inflammatory activity in acute and chronic inflammation.The activity may derive from a combination of inhibition of prostaglandin synthesis and leucocytes migration.The phytochemical constituents detected in HE and EF may account for the anti-inflammatory activity.
文摘Friable callus was induced when immature seeds of floribunda rose were inoculated on MS medium supplemented with 2,4-D 3.0 mg.L^-1. When transfered onto subculture media, friable callus developed into embryogenic callus, which was used to establish cell suspension lines. Cell suspensions had to be subcultured at a interval of 4-5 days at the first several culture cycles. The best subculturing cycle for the stable cell suspensions was 8-10 days. The best inoculum quantity was 1 mL PCV（Packed Cell Volume） per 40 mL culture fluid.
基金Supported by Special Fund for Applied Technology Research and Development and Demonstration and Promotion in Hainan Province(ZDXM2014002)~~
文摘[Objective]This study aimed to screen out floribunda rose cultivars resistant to high temperature in tropical areas.[Method]A total of 8 floribunda rose cul-tivars(Carefree Wonder,Scloss Mannheim,Bright Smile,Goldmarie,Betty Prior,Red Riding Hood Fairy Tale,Golden Scepter and Iceberg)were selected as the research objects,and their growth,adaptability and disease resistance in Sanya area were compared.[Result]Carefree Wonder,Scloss Mannheim,Iceberg and Bright Smile were suitable to be planted in Sanya area.Among them,Carefree Wonder,Scloss Mannheim and Iceberg were characterized with fewer diseases,bright colors,heat resistance and strong stress resistance,and they could be planted as ornamental flowers in Sanya area.[Conclusion]This study will provide a reference for the selection of ornamental flowers in Sanya area.
基金partially supported by the Brazilian Research Council(CNPq)(CNPq:Process No 311336/2017-5)financed in part by the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-Brasil(CAPES)-Finance Code 001.
文摘Bredemeyera floribunda Wild (Polygalaceae), also known as pau-de-ovo is a medicinal plant used to treat snakebites, as tonic and fortifiers to treat fatigue and exhaustion. The aim of the present study was to document the knowledge of herbal sellers in the preparation of the so-called “B. floribunda fortifier” and to compare the plant root content with the recommended nutritional values and the permitted limit for medicinal plants. The study revealed that, unlike other regions of Brazil, in the Midwest region, the root of the plant is used as food to treat fatigue and exhaustion. The contents in B. floribunda root decreased in the following order: Ca > K > P > Na > Fe > Mg > Si > Mn > Zn > Cu. Daily intake of 30 grams of B. floribunda roots are considered as an excellent source of Cr, Cu, Mn and Se for people 19 to 50 years of age according to the RDA. In addition, Cu and Fe showed content above the established limit for medicinal plants stipulated by other countries. The root of the plant has low K, Mg, P, Na and Zn content. When the contents in roots of the plant are below the oral dose of reference, consumption of this plant can be considered safe and without risk to human health. On the other hand, concentration of elements such as Cu, Fe, Ni, Al, Cr and Se exceeding the allowable limit may cause serious health problems due to prolonged ingestion and large amounts consumed daily. The Brazilian legislation does not establish a permissible limit of macro- and micro-elements in medicinal plants;thus, the results presented in this study will contribute to the Brazilian public health policy on medicinal plants.
文摘Germination of Lespedeza floribunda Bunge.seeds was studied in order to provide cultivation and application in gardening and landscaping.Results indicated that the seeds of Lespedeza floribunda had the characteristic of forcing dormancy that was caused by hard seed vessels.The dormancy could be released by soaking in hot water and by sulphuric acid treatment.The most appropriate treatment time was 10～30 min,resulting in average germination rate(96%),average germination power(82.53%)and average germination index(17.54).The optimum germination temperature was 25 ℃ and the germination rate,germination power and germination index was 95%,81%,22.95 respectively after treated by sulphuric acid for 20 min.
