BACKGROUND Simulated microgravity environment can lead to gastrointestinal motility disturbance.The pathogenesis of gastrointestinal motility disorders is closely related to the stem cell factor(SCF)/c-kit signaling p...BACKGROUND Simulated microgravity environment can lead to gastrointestinal motility disturbance.The pathogenesis of gastrointestinal motility disorders is closely related to the stem cell factor(SCF)/c-kit signaling pathway associated with intestinal flora and Cajal stromal cells.Moreover,intestinal flora can also affect the regulation of SCF/c-kit signaling pathway,thus affecting the expression of Cajal stromal cells.Cajal cells are the pacemakers of gastrointestinal motility.AIM To investigate the effects of Bifidobacterium lactis(B.lactis)BLa80 on the intestinal flora of rats in simulated microgravity and on the gastrointestinal motility-related SCF/c-kit pathway.METHODS The internationally recognized tail suspension animal model was used to simulate the microgravity environment,and 30 rats were randomly divided into control group,tail suspension group and drug administration tail suspension group with 10 rats in each group for a total of 28 days.The tail group was given B.lactis BLa80 by intragastric administration,and the other two groups were given water intragastric administration,the concentration of intragastric administration was 0.1 g/mL,and each rat was 1 mL/day.Hematoxylin&eosin staining was used to observe the histopathological changes in each segment of the intestine of each group,and the expression levels of SCF,c-kit,extracellular signal-regulated kinase(ERK)and p-ERK in the gastric antrum of each group were detected by Western blotting and PCR.The fecal flora and mucosal flora of rats in each group were detected by 16S rRNA.RESULTS Simulated microgravity resulted in severe exfoliation of villi of duodenum,jejunum and ileum in rats,marked damage,increased space between villi,loose arrangement,shortened columnar epithelium of colon,less folds,narrower mucosal thickness,reduced goblet cell number and crypts,and significant improvement after probiotic intervention.Simulated microgravity reduced the expressions of SCF and c-kit,and increased the expressions of ERK and P-ERK in the gastric antrum of rats.However,after probiotic intervention,the expressions of SCF and ckit were increased,while the expressions of ERK and P-ERK were decreased,with statistical significance(P<0.05).In addition,simulated microgravity can reduce the operational taxonomic unit(OTU)of the overall intestinal flora of rats,B.lactis BLa80 can increase the OTU of rats,simulated microgravity can reduce the overall richness and diversity of stool flora of rats,increase the abundance of firmicutes in stool flora of rats,and reduce the abundance of Bacteroides in stool flora of rats,most of which are mainly beneficial bacteria.Simulated microgravity can increase the overall richness and diversity of mucosal flora,increase the abundance of Bacteroides and Desulphurides in the rat mucosal flora,and decrease the abundance of firmicutes,most of which are proteobacteria.After probiotics intervention,the overall Bacteroidetes trend in simulated microgravity rats was increased.CONCLUSION B.lactis BLa80 can ameliorate intestinal mucosal injury,regulate intestinal flora,inhibit ERK expression,and activate the SCF/c-kit signaling pathway,which may have a facilitating effect on gastrointestinal motility in simulated microgravity rats.展开更多
Human wellness is the ultimate goal of our efforts in improving the human life.Special foods are undoubtedly important in achieving human wellness.However,overeating significantly leads to obesity and diabetes.These c...Human wellness is the ultimate goal of our efforts in improving the human life.Special foods are undoubtedly important in achieving human wellness.However,overeating significantly leads to obesity and diabetes.These chronic diseases will in turn affect the human wellness.Therefore,“dietary restriction and proper exercise”were introduced in the human daily life.Different foods cause various effects on the human health.The diversification of diet is a priority for nutritionists to keep our body healthy.To avoid diabetes mellitus,special foods for ketogenic diet,low-carbon diet,and low-calorie intake are also gradually attracting attention.In addition,the hypothesis that“hunger sensation comes from gut flora”brings new light to the research on the biological motivation for humans to eat food.This hypothesis has been gradually demonstrated using the flexible fasting technology by providing special foods,such as plant polysaccharides and dietary fibers.The response to food-needing signals from the gut flora to these foods demonstrates the importance of the gut flora in improving human wellness.The gut flora is probably an essential factor for translating the food-eating signals and converting the nutrition to our body.Therefore,“gut flora priority principle”is developed to guarantee human wellness.The 16S rRNA sequencing and mass spectrometric techniques can be used to identify the gut flora,which may guide us to a new era of human wellness based on gut flora wellness.展开更多
Numerous research conducted in recent years has revealed that gut microbial dysbiosis,such as modifications in composition and activity,might influence lung tissue homeostasis through specific pathways,thereby promoti...Numerous research conducted in recent years has revealed that gut microbial dysbiosis,such as modifications in composition and activity,might influence lung tissue homeostasis through specific pathways,thereby promoting susceptibility to lung diseases.The development and progression of lung cancer,as well as the effectiveness of immunotherapy are closely associated with gut flora and metabolites,which influence immunological and inflammatory responses.During abnormal proliferation,non-small cell lung cancer cells acquire more substances and energy by altering their own metabolic pathways.Glucose and amino acid metabolism reprogramming provide tumor cells with abundant ATP,carbon,and nitrogen sources,respectively,providing optimal conditions for tumor cell proliferation,invasion,and immune escape.This article reviews the relationship of immune response with gut flora and metabolic reprogramming in non-small cell lung cancer,and discusses the potential mechanisms by which gut flora and metabolic reprogramming affect the occurrence,development,and immunotherapy of non-small cell lung cancer,in order to provide new ideas for precision treatment of lung cancer patients.展开更多
The species composition and characteristics of mangrove flora in the world were reviewed and discussed. The results suggested that the world's mangrove plants have 84 species (including 12 varieties) in 24 genera ...The species composition and characteristics of mangrove flora in the world were reviewed and discussed. The results suggested that the world's mangrove plants have 84 species (including 12 varieties) in 24 genera and 16 families. Of which, true mangrove plants have 70 species (including 12 varieties) in 16 genera and 11 families, and semi-mangrove plants 14 species in eight genera and five families. The Eastern Group has 74 species (including 12 varieties) in 18 genera and 14 families, characterized by the genera Aegiceras, Osbornia, Aegialitis, Bruguiera, Ceriops, Kandelia, Scyphiphora and Nypa etc. The Western Group has only 10 species in six genera and five families, characterized by the endemic one-species family, Pelliceraceae, and the genus Laguncularia. The mangrove flora of China is composed of 26 species (including one variety) in 15 genera and 12 families, four of which are endemic. Hainan is most rich in mangrove species, making up about 96.2% of the Chinese total; Guangdong ranks second, making up about 42.3%. It has been demonstrated that Rhizophora stylosa was mistaken for R. mucronata in Taiwan by previous authors.展开更多
Objective:To explore the pharmacological effects and molecular mechanisms of a Jiao'ai decoction(JAD)in treating premature ovarian failure.Specifically,we evaluated the receptor for advanced glycation end(RAGE)pro...Objective:To explore the pharmacological effects and molecular mechanisms of a Jiao'ai decoction(JAD)in treating premature ovarian failure.Specifically,we evaluated the receptor for advanced glycation end(RAGE)products pathway,metabolic disorders,and intestinal flora dysbiosis.Methods:Forty female rats with normal estrous cycles were randomly divided into five groups:control,model,estradiol,low-dose JAD,and high-dose JAD,with 8 rats in each.Except for the control group,the rats in other groups were injected intraperitoneally with cisplatin for 8 days(1.5 mg/kg)to establish a premature ovarian failure model.Starting on the fifth day of cisplatin injections,the estradiol,low-dose JAD,and high-dose JAD groups were administered corresponding drugs for 21 days.Sex hormone levels and pathological changes in the ovaries were measured.Key proteins in the RAGE pathway related to apoptosis,aging,and inflammation,were tested using Western blot.A 16S rRNA analysis of feces and non-targeted metabolism in serum was performed to determine the effects of JAD on intestinal flora and metabolism.Results:Body weight,ovarian index,and the number of follicles at all levels increased in the JAD group.Regarding serum hormones,estradiol,anti-mullerian hormone(AMH)and P levels increased,whereas follicle-stimulating hormone(FSH)and luteinizing hormone(LH)levels decreased in the JAD group.The levels of phosphorylated Akt protein(P-Akt),receptor for advanced glycation end products(RAGE),tumor protein p53(P53),C-reactive protein(CRP),apoptosis regulator BAX(BAX)and Caspase3 were downregulated by JAD,whereas B cell leukemia/lymphoma 2(Bcl-2)and Endothelial nitric oxidase synthase(eNOS)were upregulated.JAD was also found to play an important role in the regulation of metabolic disorders and intestinal ecological imbalances by adjusting species composition and diversity.Conclusion:JAD can protect ovaries by exerting anti-inflammatory and anti-apoptotic effects via inhibition of the RAGE pathway.JAD can also regulate metabolic disorders and maintain the dynamic balance of intestinal flora,thereby contributing to the improvement of the ovarian reserve function.展开更多
Background:Liver injury often occurs but with limited drugs.Chaenomeles has a potent hepatoprotective effect,while the ability of Chaenomeles speciosa and Chaenomeles sinensis to treat liver injury in rats is unexplor...Background:Liver injury often occurs but with limited drugs.Chaenomeles has a potent hepatoprotective effect,while the ability of Chaenomeles speciosa and Chaenomeles sinensis to treat liver injury in rats is unexplored.Methods:The study involved 30 rats divided into five groups:negative control(NC),model control(MC),positive control(PC),Chaenomeles speciosa-delivered(ZP),and Chaenomeles sinensis-delivered(GP).Fecal samples from all groups were collected 24 h post-modeling for intestinal flora analysis.All rats were collected serum and liver tissues for biochemical and histopathological examinations,among other experiments.Chaenomeles would be effective in CCl4-induced liver injury in rats by analyzing the efficacy and mechanism and examining the differences between Chaenomeles speciosa and Chaenomeles sinensis through oxidative stress,inflammation,and apoptosis pathways.Then,we resolved the mechanism of action in the context of the intestinal flora.Results:The results showed that Chaenomeles intake improved the degree of CCl4-induced liver injury,decreased aspartate aminotransferase,alanine aminotransferase,and alkaline phosphatase levels,and increased total protein and total bilirubin levels.Noteworthy,the glutathione level in the GP group surpassed that a 1.5-fold increase compared to the PC group.Chaenomeles speciosa could exert its efficacy by regulating inflammatory and apoptotic pathways,while Chaenomeles speciosa did so through the oxidative stress pathway.In addition,Chaenomeles are both able to modulate intestinal flora and change the ratio of flora.Chaenomeles speciosa could regulate probiotics and prevent liver injury by altering the distribution and ratio of intestinal flora.Specifically,Lactobacillaceae in the ZP group exhibited 10-fold higher abundance than the other groups.Chaenomeles speciosa increased the abundance of probiotic Clostridiales butyricum in diseased rats,while Chaenomeles sinensis increased the abundance of pathogenic Escherichia Shigella.Conclusion:This study suggests that Chaenomeles may be hepatoprotective by oxidative stress,inflammation,and apoptosis pathways and modulating the composition and function of the intestinal flora.展开更多
The anti-inflammatory properties of silkworm pupa peptide(SPP)have been recognized for their potential benefits in colitis treatment.However,the underlying mechanism of SPP in colitis animal models remains unclear.The...The anti-inflammatory properties of silkworm pupa peptide(SPP)have been recognized for their potential benefits in colitis treatment.However,the underlying mechanism of SPP in colitis animal models remains unclear.The objective of our work was to investigate the improving effect and the mechanism of action of SPP on a mouse model of dextran sulfate sodium(DSS)-induced colitis.The findings suggested that SPP effectively eased ulcerative colitis(UC)symptoms in mice by decreasing disease activity index,ameliorating histopathological injury,and decreasing serum and colonic markers of inflammation.In addition,our research findings demonstrated that SPP restored intestinal barrier function by increasing the production of tight junction proteins such as zonula occludens-1(ZO-1),claudin-1,claudin-3,occludin,and adherens junction protein E-cadherin1.Further,SPP supplementation enhanced the concentration of short-chain fatty acids and positively altered the makeup of the gut bacteria in the mice's gut.These findings underscore SPP's ability to slow the progression of colitis and point to its possible use as a functional component in dietary supplements for the prevention of early-stage colitis.展开更多
BACKGROUND The intestinal flora(IF)has been linked to risks of non-communicable diseases,especially various cancers,stroke,and Alzheimer’s disease.However,many uncertainties of these associations during different sta...BACKGROUND The intestinal flora(IF)has been linked to risks of non-communicable diseases,especially various cancers,stroke,and Alzheimer’s disease.However,many uncertainties of these associations during different stages of growth,deve-lopment,and aging still exist.Therefore,further in-depth explorations are warranted.AIM To explore the associations of the human IF with disease risks during different stages of growth,development,and aging to achieve more accurate and con-vincing conclusions.METHODS Cohort,cross-sectional,case-control,and Mendelian randomization studies published in the PubMed and Web of Science databases until December 31,2023 were systematically reviewed to clarify the associations of the IF at the genus level with the risks of various non-communicable diseases,which were grouped in accordance with the 10^(th) revision of the International Classification of Diseases.RESULTS In total,57 studies were included to quantitatively examine the influence of the IF on the risks of 30 non-communicable diseases during different stages of growth,development,and aging.Population studies and Mendelian randomization studies confirmed positive associations of the abundances of Bifidobacterium and Ruminococcus with multiple sclerosis.CONCLUSION These findings contribute to a deeper understanding of the roles of the IF and provide novel evidence for effective strategies for the prevention and treatment of non-communicable diseases.In the future,it will be necessary to explore a greater variety of research techniques to uncover the specific mechanisms by which gut microbiota trigger diseases and conduct in-depth studies on the temporal relationship between microbiota alterations and diseases,so as to clarify the causal relationship more accurately.展开更多
Objective:To investigate the clinical efficacy of Modified Gegen Qinlian Decoction combined with probiotics(Clostridium butyricum live capsules)in treating radiation enteritis(RE)in tumor patients,as well as its regul...Objective:To investigate the clinical efficacy of Modified Gegen Qinlian Decoction combined with probiotics(Clostridium butyricum live capsules)in treating radiation enteritis(RE)in tumor patients,as well as its regulatory effect on the structure of intestinal flora,providing clinical evidence for the integrated traditional Chinese and Western medicine treatment of radiation enteritis.Methods:A total of 40 patients with radiation enteritis admitted to the Oncology Department of Linfen Central Hospital from September 2023 to December 2024 were selected and divided into an observation group and a control group according to the random number table method,with 20 cases in each group.The control group was treated with Clostridium butyricum live capsules,while the observation group was treated with Modified Gegen Qinlian Decoction in addition to the treatment given to the control group.Both groups received a 4-week treatment course.Intestinal function indicators and changes in intestinal flora structure were compared between the two groups before and after treatment,and clinical efficacy was evaluated.Results:After 4 weeks of treatment,the total effective rate in the observation group versus the control group was(95.00%vs 65.00%),with a statistically significant difference(P<0.05).The fecal formation rate in the observation group(85.00%vs 60.00%)was significantly higher than that in the control group,and the defecation frequency(2.15±0.42 vs 3.85±0.65)times/day was significantly lower than that in the control group,with statistically significant differences(P<0.05).After treatment,the quantities of Bifidobacterium(6.85±0.72 L vs 5.23±0.61 L)gCFU/g and Lactobacillus(6.52±0.68 L vs 4.98±0.57 L)gCFU/g in the observation group were significantly higher than those in the control group,while the quantities of Escherichia coli(4.12±0.53 L vs 5.67±0.65 L)gCFU/g and Staphylococcus(3.85±0.48 L vs 5.23±0.59 L)gCFU/g were significantly lower than those in the control group,with statistically significant differences(P<0.05).Conclusion:Clinical studies have confirmed that the synergistic treatment of radiation enteritis with Modified Gegen Qinlian Decoction and probiotics can significantly improve patients’clinical symptoms and restore the balance of intestinal flora,providing an effective regimen for clinical treatment.展开更多
This study established a rapid extraction method for the 16S rRNA gene of intestinal flora.Combined with nextgeneration sequencing(NGS)technology,this method can be applied to intestinal microbial ecology analysis.The...This study established a rapid extraction method for the 16S rRNA gene of intestinal flora.Combined with nextgeneration sequencing(NGS)technology,this method can be applied to intestinal microbial ecology analysis.The fecal sample was mixed with lysis buffer,incubated at 90°C for 15 minutes,vortexed,and then centrifuged.The supernatant was collected,and specific primers were added for PCR amplification.The PCR products were purified,and index tags were established for the targets to obtain libraries.After library purification and quality inspection,the libraries could be loaded for sequencing.Under optimized conditions:when the number of PCR cycles was 25 and the initial amount of DNA for library construction was 12.5 ng,fewer chimeras were generated.The library size was 500-700 bp,and there were no primer dimers of approximately 120 bp.This method is rapid,accurate,and sensitive,and can be used for the analysis and detection of the 16S rRNA gene of intestinal flora.展开更多
Background:Intratumoral flora and its metabolites play an important role in the occurrence,development and treatment of cancer,and are correlated with the genotype expression of breast cancer;However,the internal rela...Background:Intratumoral flora and its metabolites play an important role in the occurrence,development and treatment of cancer,and are correlated with the genotype expression of breast cancer;However,the internal relationship between intratumoral flora and triple negative breast cancer(TNBC)has not been elucidated.Methods:Fourteen patients with TNBC who met the criteria were included.The tumor tissues and adjacent tissues were respectively taken as the patient group and the control group.The 5R 16S sequencing technique was used to detect the abundance and distribution of the intratumoral flora between the two groups,and the differences between the groups were analyzed to find the bacteria with significant differences between groups(P<0.05).Results:The abundance of intratumoral microbiota in TNBC patients was significantly lower than that in adjacent tissues(P<0.05).The differential bacteria in TNBC tumors(P<0.05)included Acinetobacter,Renibacterium,Flavobacterium,Dechloromonas and others.The differential bacteria genera(P<0.05)in the adjacent tissues included Comamonas,Bacillus,Caulobacter,Afipia,Aerococcus,Roseomonas and so on.Conclusion:There is a significant difference in the flora structure between the tumor and normal tissues in TNBC patients.Proteobacteria plays an important role in the occurrence,development and treatment of TNBC.Among them,Acinetobacter may be the key reason for the metastasis of TNBC.展开更多
The investigation and analysis of woody plants on the campus of Anhui Xinhua University reveals the presence of 103 species distributed across 43 families and 78 genera.Notably,depauperate families and monotypic gener...The investigation and analysis of woody plants on the campus of Anhui Xinhua University reveals the presence of 103 species distributed across 43 families and 78 genera.Notably,depauperate families and monotypic genera are found to be the most numerous.Among the families,Rosaceae is identified as the most diverse,encompassing a total of 21 species,while Prunus is found to contain the highest number of species,totaling 5.The geographic component of the plant flora is intricate and encompasses a wide range of distribution types,with a predominance of both tropic and temperate plants.展开更多
AIM:To assess and compare the conjunctival bacterial flora in patients with congenital ptosis(CP)to that of healthy individuals.METHODS:The study included 38 patients with CP and 42 healthy control subjects.Conjunctiv...AIM:To assess and compare the conjunctival bacterial flora in patients with congenital ptosis(CP)to that of healthy individuals.METHODS:The study included 38 patients with CP and 42 healthy control subjects.Conjunctival cultures were collected using a cotton-tipped swab applied to the inferior conjunctival fornix.The samples were inoculated into blood agar,eosin methylene blue agar,chocolate agar for bacteria,and Sabouraud dextrose agar medium for fungi.RESULTS:The culture positivity rate was 68.4%in CP and 47.6%in the control group(P<0.001).Additionally,there was a significant difference in culture positivity between the eyes of patients with unilateral ptosis(P=0.039).In the CP group,the most common found microorganism was Staphylococcus epidermidis at 23.67%,followed by Haemophilus species at 21.04%,and Corynebacterium species at 15.78%.In the control group,Staphylococcus epidermidis and Corynebacterium species were both the most commonly isolated microorganisms,each accounting for 19.04%.More than one species of bacteria was grown in the cultures of 36.84%and 23.80%of the CP and control subjects,respectively.Gram-negative bacteria were more common in CP than in control subjects(P=0.031).CONCLUSION:Culture positivity is significantly higher in the ptotic eyes of CP.Potentially pathogenic microorganisms are more frequently isolated from the CP group compared with the healthy control group.展开更多
The gut microbiota and it’s metabolism are vital targets of probiotics regulating high fat-diet(HFD)induced hyperlipidemia,which can relieve the pressure caused by the striking growth of sub-health people.Pediococcus...The gut microbiota and it’s metabolism are vital targets of probiotics regulating high fat-diet(HFD)induced hyperlipidemia,which can relieve the pressure caused by the striking growth of sub-health people.Pediococcus pentosaceus PP04(PP04)could colonize in intestine to regulate gut microbiota and it’s metabolites directly,the rebalanced intestinal flora mediated by PP04 could facilitate the secretion of short chain fatty acids to control body weight gain,PP04 intervention also changed bile acid(BA)profiles and enhanced the ileal concentrations of antagonists including tauro-α/β-muricholic acid sodium salt and ursodeoxycholic acid to inhibit intestinal farnesoid X receptor/fibroblast growth factor 15(FXR/FGF15)signaling coupled with the activation of hepatic FXR/small heterodimer partners signaling,which accelerated the hepatic BA de novo synthesis and excretion with feces to eliminate HFD caused hyperlipemia effectively.This study provided important evidence regarding PP04 as dietary supplement to relieve hyperlipidemia by influencing BA enterohepatic circulation.展开更多
The Andaman Islands,part of the Indo-Burma biodiversity hotspot,holds unique florawith many endemic and threatened species.However,this fragile diversity is under increasing pressure from infrastructure expansion,clim...The Andaman Islands,part of the Indo-Burma biodiversity hotspot,holds unique florawith many endemic and threatened species.However,this fragile diversity is under increasing pressure from infrastructure expansion,climate change and habitat fragmentation.The recently announced Great Nicobar Island Development project by the Government of India,which includes an international transhipment terminal,airport,township and power plant,if implemented,would cause major habitat loss and fragmentation,possibly leading to species extinctions.展开更多
Thymus quinquecostatus Celak.,a traditional aromatic edible plant from Lamiaceae,is widely used as food additive,food condiment,spice,and herbal teas.Polyphenol-rich fraction of T.quinquecostatus(PRF)has been proven t...Thymus quinquecostatus Celak.,a traditional aromatic edible plant from Lamiaceae,is widely used as food additive,food condiment,spice,and herbal teas.Polyphenol-rich fraction of T.quinquecostatus(PRF)has been proven to be effective protective effect for cerebral ischemia reperfusion injury(CIRI)in our previous study.In this study,we developed a novel“Gut flora-Compound-Target-Pathway”(GCTP)network based on network pharmacology coupled with gastrointestinal metabolism for screening bio-active components,key targets and gut floras through the classical technique for order preference by similarity to ideal solution(TOPSIS).This compensates for the lack of gut floras and gastrointestinal metabolism in network pharmacology.Firstly,four incubation models covering simulated gastric juice,simulated intestinal juice,gut floras of normal and transient middle cerebral artery occlusion(tMCAO)rat in vitro were applied to PRF.The 109 proto-components and 64 metabolites were elucidated by ultra-high performance liquid chromatography Q exactive orbitrap-mass spectrometry(UPLC-QE-Orbitrap-MS).Then,the key targets of matrix metalloproteinase 9(MMP9),prostaglandin-endoperoxide synthase 2(PTGS2),tyrosine-protein kinase fyn(FYN),estrogen receptor 1(ESR1),amyloid precursor protein(APP),and epidermal growth factor receptor(EGFR),and gut floras of Enterococcus avium LY1 were selected.Moreover,the selected key proto components were rosmarinic acid,daidzein,quercetin,luteolin,apigenin,methyl rosmarinate,kaempferol,luteoloside,and caffeic acid,and the key metabolites were isokaempferide,isorhamnetin,isoquercetin,and mangiferin.Binding of compounds to the key proteins was analyzed by molecular docking,and also verified though an 2,2'-azobis(2-amidinopropane)dihydrochloride(AAPH)induced oxidative stress zebrafish model and real-time quantitative polymerase chain reaction(RT-qPCR)assays.This study provides a new idea and a better understanding of PRF for its protective effects on CIRI and its underlying mechanisms.展开更多
According to the investigation of sampling area of 6800 m2 on the south slope of Shennongjia Mountain, there were 126 vascular plant species, belonging to 108 genera and 64 families, in the investigated rare plant com...According to the investigation of sampling area of 6800 m2 on the south slope of Shennongjia Mountain, there were 126 vascular plant species, belonging to 108 genera and 64 families, in the investigated rare plant communities, of which 9 rare plant species were recorded, accounting for 27.3% of the total rare plants. The communities were about 30 m in height and were divided into three layers as tree layer, shrub layer, and herb layer. The flora of the communities had obvious temperate character. Phanerophytes (accounted for 65.9%), Mesophyllous (62.7%), Papyraceous (84.1%), simple leaf (83.3%), un-entire leaf (69.8%) were dominant in life form, leaf size class, leaf texture, leaf form, and leaf margin respectively. According to important value of species, the communities were divided into three types as Davidia involucrata + Litsea pungens community, Cercidiphyllum japanicum + Padus wilsonii community, and Padus wilsonii + Acer mono community. The indexes of species diversity of tree layer had little difference among communities and evenness was high. The results indicated that the communities had complex structure and relative stability.展开更多
Based on the thorough investigation of the components of urban forest in Nanjing and the collection of samples,it is concluded that there are 1 500 species(including subspecies) of vascular plants belonging to 572 gen...Based on the thorough investigation of the components of urban forest in Nanjing and the collection of samples,it is concluded that there are 1 500 species(including subspecies) of vascular plants belonging to 572 genera of 172 families,among which there are 63 species of ferns belonging to 36 genera of 25 families,1 437 species of seed plants belonging to 536 genera of 147 families.The characteristics are that the flora is rich in species,complex in geographic composition,and possess the distinct transferring quality from tropical to temperate zone.Besides,it is characterized with certain ancient nature,and the peculiar elements are comparatively simple.展开更多
The tropical flora in southern China is represented mainly by three regional flora, i.e. southern Yunnan (southwestern China), southwestern Guangxi (southwestern China) and Hainan Island (southern China). The floristi...The tropical flora in southern China is represented mainly by three regional flora, i.e. southern Yunnan (southwestern China), southwestern Guangxi (southwestern China) and Hainan Island (southern China). The floristic composition of each is concisely enumerated. Their geographical elements at generic level are analyzed. Furthermore, a comparison of floristic similarities between southern Yunnan and Hainan Island and Malay Peninsula and Brunei of western Malesia is made, based on existing regional flora treatments. The following is concluded: (1) The flora of southern China consists mainly of tropical floristic elements which contribute about 60% at the family level and more than 80 % at the generic level of its total flora. The dominant distribution type of the flora of southern China at the generic level is the tropical Asian distribution pattern. This reveals that the flora of southern China is of tropical nature with a strong tropical Asian affinity; (2) Most of the dominant families from the flora of southern China are also dominant in the Malesian flora. The floristic similarities between the regional flora of southern China and the regional flora of western Malesia are over 70% at the family level and more than 50% at the generic level. This suggests that the tropical flora of southern China belongs to the Malesian floristic region; (3) Situated at the northern margin of tropical Asia, on the other hand it is also obvious that the flora of southern China comprises less strictly tropical elements compared to the Malesian flora, and is consequently only a marginal type of the latter; (4) The close affinity of the flora of southern China to the Malesian flora can be explained by the geological history of southeast Asia.展开更多
To investigate the effects of different vegetable growing regions and planting modes on soil quality,soils in high,medium and low altitude areas of Guizhou were respectively sampled under different vegetable efficient...To investigate the effects of different vegetable growing regions and planting modes on soil quality,soils in high,medium and low altitude areas of Guizhou were respectively sampled under different vegetable efficient planting modes,and the variations of soil microbial flora and enzyme activities were analyzed. The soil microbial count and total bacteria of the vegetable efficient cultivation mode were significantly higher than that of the control （traditional planting mode） in each planting area,and the microbial diversity index was also improved to varying de- grees.The soil phosphatase,catalase and urease activities of the vegetable efficient planting mode were higher than that of the control.The soil catalase and urease activities were higher than that of the control by 1.37-1.44 and 1.51-2.80 times. Application of vegetable efficient planting mode in different regions will help to im- prove the soil quality in a given period.展开更多
文摘BACKGROUND Simulated microgravity environment can lead to gastrointestinal motility disturbance.The pathogenesis of gastrointestinal motility disorders is closely related to the stem cell factor(SCF)/c-kit signaling pathway associated with intestinal flora and Cajal stromal cells.Moreover,intestinal flora can also affect the regulation of SCF/c-kit signaling pathway,thus affecting the expression of Cajal stromal cells.Cajal cells are the pacemakers of gastrointestinal motility.AIM To investigate the effects of Bifidobacterium lactis(B.lactis)BLa80 on the intestinal flora of rats in simulated microgravity and on the gastrointestinal motility-related SCF/c-kit pathway.METHODS The internationally recognized tail suspension animal model was used to simulate the microgravity environment,and 30 rats were randomly divided into control group,tail suspension group and drug administration tail suspension group with 10 rats in each group for a total of 28 days.The tail group was given B.lactis BLa80 by intragastric administration,and the other two groups were given water intragastric administration,the concentration of intragastric administration was 0.1 g/mL,and each rat was 1 mL/day.Hematoxylin&eosin staining was used to observe the histopathological changes in each segment of the intestine of each group,and the expression levels of SCF,c-kit,extracellular signal-regulated kinase(ERK)and p-ERK in the gastric antrum of each group were detected by Western blotting and PCR.The fecal flora and mucosal flora of rats in each group were detected by 16S rRNA.RESULTS Simulated microgravity resulted in severe exfoliation of villi of duodenum,jejunum and ileum in rats,marked damage,increased space between villi,loose arrangement,shortened columnar epithelium of colon,less folds,narrower mucosal thickness,reduced goblet cell number and crypts,and significant improvement after probiotic intervention.Simulated microgravity reduced the expressions of SCF and c-kit,and increased the expressions of ERK and P-ERK in the gastric antrum of rats.However,after probiotic intervention,the expressions of SCF and ckit were increased,while the expressions of ERK and P-ERK were decreased,with statistical significance(P<0.05).In addition,simulated microgravity can reduce the operational taxonomic unit(OTU)of the overall intestinal flora of rats,B.lactis BLa80 can increase the OTU of rats,simulated microgravity can reduce the overall richness and diversity of stool flora of rats,increase the abundance of firmicutes in stool flora of rats,and reduce the abundance of Bacteroides in stool flora of rats,most of which are mainly beneficial bacteria.Simulated microgravity can increase the overall richness and diversity of mucosal flora,increase the abundance of Bacteroides and Desulphurides in the rat mucosal flora,and decrease the abundance of firmicutes,most of which are proteobacteria.After probiotics intervention,the overall Bacteroidetes trend in simulated microgravity rats was increased.CONCLUSION B.lactis BLa80 can ameliorate intestinal mucosal injury,regulate intestinal flora,inhibit ERK expression,and activate the SCF/c-kit signaling pathway,which may have a facilitating effect on gastrointestinal motility in simulated microgravity rats.
基金This work was supported by the National Basic Research Project(973 program,2012CB518200)General Program(81371232,81573251)of the Natural Science Foundation of China+1 种基金Special Key Programs for Drug R&D of China(2012ZX09102301-016,2014ZX09J14107-05B)Foundation of Joint Research Center for Translational Medicine between Beijing Proteome Research Center and Tianjin Baodi Hospital(TMRC201301).
文摘Human wellness is the ultimate goal of our efforts in improving the human life.Special foods are undoubtedly important in achieving human wellness.However,overeating significantly leads to obesity and diabetes.These chronic diseases will in turn affect the human wellness.Therefore,“dietary restriction and proper exercise”were introduced in the human daily life.Different foods cause various effects on the human health.The diversification of diet is a priority for nutritionists to keep our body healthy.To avoid diabetes mellitus,special foods for ketogenic diet,low-carbon diet,and low-calorie intake are also gradually attracting attention.In addition,the hypothesis that“hunger sensation comes from gut flora”brings new light to the research on the biological motivation for humans to eat food.This hypothesis has been gradually demonstrated using the flexible fasting technology by providing special foods,such as plant polysaccharides and dietary fibers.The response to food-needing signals from the gut flora to these foods demonstrates the importance of the gut flora in improving human wellness.The gut flora is probably an essential factor for translating the food-eating signals and converting the nutrition to our body.Therefore,“gut flora priority principle”is developed to guarantee human wellness.The 16S rRNA sequencing and mass spectrometric techniques can be used to identify the gut flora,which may guide us to a new era of human wellness based on gut flora wellness.
基金supported by the Scientific Research Fund Project of Education Department of Yunnan Province,China(No.2024Y386).
文摘Numerous research conducted in recent years has revealed that gut microbial dysbiosis,such as modifications in composition and activity,might influence lung tissue homeostasis through specific pathways,thereby promoting susceptibility to lung diseases.The development and progression of lung cancer,as well as the effectiveness of immunotherapy are closely associated with gut flora and metabolites,which influence immunological and inflammatory responses.During abnormal proliferation,non-small cell lung cancer cells acquire more substances and energy by altering their own metabolic pathways.Glucose and amino acid metabolism reprogramming provide tumor cells with abundant ATP,carbon,and nitrogen sources,respectively,providing optimal conditions for tumor cell proliferation,invasion,and immune escape.This article reviews the relationship of immune response with gut flora and metabolic reprogramming in non-small cell lung cancer,and discusses the potential mechanisms by which gut flora and metabolic reprogramming affect the occurrence,development,and immunotherapy of non-small cell lung cancer,in order to provide new ideas for precision treatment of lung cancer patients.
文摘The species composition and characteristics of mangrove flora in the world were reviewed and discussed. The results suggested that the world's mangrove plants have 84 species (including 12 varieties) in 24 genera and 16 families. Of which, true mangrove plants have 70 species (including 12 varieties) in 16 genera and 11 families, and semi-mangrove plants 14 species in eight genera and five families. The Eastern Group has 74 species (including 12 varieties) in 18 genera and 14 families, characterized by the genera Aegiceras, Osbornia, Aegialitis, Bruguiera, Ceriops, Kandelia, Scyphiphora and Nypa etc. The Western Group has only 10 species in six genera and five families, characterized by the endemic one-species family, Pelliceraceae, and the genus Laguncularia. The mangrove flora of China is composed of 26 species (including one variety) in 15 genera and 12 families, four of which are endemic. Hainan is most rich in mangrove species, making up about 96.2% of the Chinese total; Guangdong ranks second, making up about 42.3%. It has been demonstrated that Rhizophora stylosa was mistaken for R. mucronata in Taiwan by previous authors.
基金sponsored by the National Natural Science Foundation of China(81673764)National Key R&D Program of China(022YFC3502301).
文摘Objective:To explore the pharmacological effects and molecular mechanisms of a Jiao'ai decoction(JAD)in treating premature ovarian failure.Specifically,we evaluated the receptor for advanced glycation end(RAGE)products pathway,metabolic disorders,and intestinal flora dysbiosis.Methods:Forty female rats with normal estrous cycles were randomly divided into five groups:control,model,estradiol,low-dose JAD,and high-dose JAD,with 8 rats in each.Except for the control group,the rats in other groups were injected intraperitoneally with cisplatin for 8 days(1.5 mg/kg)to establish a premature ovarian failure model.Starting on the fifth day of cisplatin injections,the estradiol,low-dose JAD,and high-dose JAD groups were administered corresponding drugs for 21 days.Sex hormone levels and pathological changes in the ovaries were measured.Key proteins in the RAGE pathway related to apoptosis,aging,and inflammation,were tested using Western blot.A 16S rRNA analysis of feces and non-targeted metabolism in serum was performed to determine the effects of JAD on intestinal flora and metabolism.Results:Body weight,ovarian index,and the number of follicles at all levels increased in the JAD group.Regarding serum hormones,estradiol,anti-mullerian hormone(AMH)and P levels increased,whereas follicle-stimulating hormone(FSH)and luteinizing hormone(LH)levels decreased in the JAD group.The levels of phosphorylated Akt protein(P-Akt),receptor for advanced glycation end products(RAGE),tumor protein p53(P53),C-reactive protein(CRP),apoptosis regulator BAX(BAX)and Caspase3 were downregulated by JAD,whereas B cell leukemia/lymphoma 2(Bcl-2)and Endothelial nitric oxidase synthase(eNOS)were upregulated.JAD was also found to play an important role in the regulation of metabolic disorders and intestinal ecological imbalances by adjusting species composition and diversity.Conclusion:JAD can protect ovaries by exerting anti-inflammatory and anti-apoptotic effects via inhibition of the RAGE pathway.JAD can also regulate metabolic disorders and maintain the dynamic balance of intestinal flora,thereby contributing to the improvement of the ovarian reserve function.
基金supported by the Key Project at the Central Government Level(No.2060302),the Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine(No.ZYYCXTD-D-202005),and the National Natural Science Foundation of China(No.8187295682173929).
文摘Background:Liver injury often occurs but with limited drugs.Chaenomeles has a potent hepatoprotective effect,while the ability of Chaenomeles speciosa and Chaenomeles sinensis to treat liver injury in rats is unexplored.Methods:The study involved 30 rats divided into five groups:negative control(NC),model control(MC),positive control(PC),Chaenomeles speciosa-delivered(ZP),and Chaenomeles sinensis-delivered(GP).Fecal samples from all groups were collected 24 h post-modeling for intestinal flora analysis.All rats were collected serum and liver tissues for biochemical and histopathological examinations,among other experiments.Chaenomeles would be effective in CCl4-induced liver injury in rats by analyzing the efficacy and mechanism and examining the differences between Chaenomeles speciosa and Chaenomeles sinensis through oxidative stress,inflammation,and apoptosis pathways.Then,we resolved the mechanism of action in the context of the intestinal flora.Results:The results showed that Chaenomeles intake improved the degree of CCl4-induced liver injury,decreased aspartate aminotransferase,alanine aminotransferase,and alkaline phosphatase levels,and increased total protein and total bilirubin levels.Noteworthy,the glutathione level in the GP group surpassed that a 1.5-fold increase compared to the PC group.Chaenomeles speciosa could exert its efficacy by regulating inflammatory and apoptotic pathways,while Chaenomeles speciosa did so through the oxidative stress pathway.In addition,Chaenomeles are both able to modulate intestinal flora and change the ratio of flora.Chaenomeles speciosa could regulate probiotics and prevent liver injury by altering the distribution and ratio of intestinal flora.Specifically,Lactobacillaceae in the ZP group exhibited 10-fold higher abundance than the other groups.Chaenomeles speciosa increased the abundance of probiotic Clostridiales butyricum in diseased rats,while Chaenomeles sinensis increased the abundance of pathogenic Escherichia Shigella.Conclusion:This study suggests that Chaenomeles may be hepatoprotective by oxidative stress,inflammation,and apoptosis pathways and modulating the composition and function of the intestinal flora.
基金supported by the National Key Research and Development Program of China(2023YFF1103802)。
文摘The anti-inflammatory properties of silkworm pupa peptide(SPP)have been recognized for their potential benefits in colitis treatment.However,the underlying mechanism of SPP in colitis animal models remains unclear.The objective of our work was to investigate the improving effect and the mechanism of action of SPP on a mouse model of dextran sulfate sodium(DSS)-induced colitis.The findings suggested that SPP effectively eased ulcerative colitis(UC)symptoms in mice by decreasing disease activity index,ameliorating histopathological injury,and decreasing serum and colonic markers of inflammation.In addition,our research findings demonstrated that SPP restored intestinal barrier function by increasing the production of tight junction proteins such as zonula occludens-1(ZO-1),claudin-1,claudin-3,occludin,and adherens junction protein E-cadherin1.Further,SPP supplementation enhanced the concentration of short-chain fatty acids and positively altered the makeup of the gut bacteria in the mice's gut.These findings underscore SPP's ability to slow the progression of colitis and point to its possible use as a functional component in dietary supplements for the prevention of early-stage colitis.
基金Supported by National Natural Science Foundation of China,No.81903398the Research Start-Up Fund for the Introduction of Talents of Sichuan University,No.YJ2021112+4 种基金Medical Youth Innovation Research Project of Sichuan Province,No.Q21016Natural Science Foundation of Sichuan,No.2023NSFSC1927“From 0 to 1”Innovation Project,Sichuan University,No.2023SCUH0026Sichuan Provincial Science and Technology Department 2023 Central Guide Local Project,No.2023ZYD0097Cigar Fermentation Technology Key Laboratory of Tobacco Industry,No.20202309BC530.
文摘BACKGROUND The intestinal flora(IF)has been linked to risks of non-communicable diseases,especially various cancers,stroke,and Alzheimer’s disease.However,many uncertainties of these associations during different stages of growth,deve-lopment,and aging still exist.Therefore,further in-depth explorations are warranted.AIM To explore the associations of the human IF with disease risks during different stages of growth,development,and aging to achieve more accurate and con-vincing conclusions.METHODS Cohort,cross-sectional,case-control,and Mendelian randomization studies published in the PubMed and Web of Science databases until December 31,2023 were systematically reviewed to clarify the associations of the IF at the genus level with the risks of various non-communicable diseases,which were grouped in accordance with the 10^(th) revision of the International Classification of Diseases.RESULTS In total,57 studies were included to quantitatively examine the influence of the IF on the risks of 30 non-communicable diseases during different stages of growth,development,and aging.Population studies and Mendelian randomization studies confirmed positive associations of the abundances of Bifidobacterium and Ruminococcus with multiple sclerosis.CONCLUSION These findings contribute to a deeper understanding of the roles of the IF and provide novel evidence for effective strategies for the prevention and treatment of non-communicable diseases.In the future,it will be necessary to explore a greater variety of research techniques to uncover the specific mechanisms by which gut microbiota trigger diseases and conduct in-depth studies on the temporal relationship between microbiota alterations and diseases,so as to clarify the causal relationship more accurately.
基金Four“batches”innovation project of invigorating medicine through science and technology of Shanxi province(Project No.:2023XM059)。
文摘Objective:To investigate the clinical efficacy of Modified Gegen Qinlian Decoction combined with probiotics(Clostridium butyricum live capsules)in treating radiation enteritis(RE)in tumor patients,as well as its regulatory effect on the structure of intestinal flora,providing clinical evidence for the integrated traditional Chinese and Western medicine treatment of radiation enteritis.Methods:A total of 40 patients with radiation enteritis admitted to the Oncology Department of Linfen Central Hospital from September 2023 to December 2024 were selected and divided into an observation group and a control group according to the random number table method,with 20 cases in each group.The control group was treated with Clostridium butyricum live capsules,while the observation group was treated with Modified Gegen Qinlian Decoction in addition to the treatment given to the control group.Both groups received a 4-week treatment course.Intestinal function indicators and changes in intestinal flora structure were compared between the two groups before and after treatment,and clinical efficacy was evaluated.Results:After 4 weeks of treatment,the total effective rate in the observation group versus the control group was(95.00%vs 65.00%),with a statistically significant difference(P<0.05).The fecal formation rate in the observation group(85.00%vs 60.00%)was significantly higher than that in the control group,and the defecation frequency(2.15±0.42 vs 3.85±0.65)times/day was significantly lower than that in the control group,with statistically significant differences(P<0.05).After treatment,the quantities of Bifidobacterium(6.85±0.72 L vs 5.23±0.61 L)gCFU/g and Lactobacillus(6.52±0.68 L vs 4.98±0.57 L)gCFU/g in the observation group were significantly higher than those in the control group,while the quantities of Escherichia coli(4.12±0.53 L vs 5.67±0.65 L)gCFU/g and Staphylococcus(3.85±0.48 L vs 5.23±0.59 L)gCFU/g were significantly lower than those in the control group,with statistically significant differences(P<0.05).Conclusion:Clinical studies have confirmed that the synergistic treatment of radiation enteritis with Modified Gegen Qinlian Decoction and probiotics can significantly improve patients’clinical symptoms and restore the balance of intestinal flora,providing an effective regimen for clinical treatment.
文摘This study established a rapid extraction method for the 16S rRNA gene of intestinal flora.Combined with nextgeneration sequencing(NGS)technology,this method can be applied to intestinal microbial ecology analysis.The fecal sample was mixed with lysis buffer,incubated at 90°C for 15 minutes,vortexed,and then centrifuged.The supernatant was collected,and specific primers were added for PCR amplification.The PCR products were purified,and index tags were established for the targets to obtain libraries.After library purification and quality inspection,the libraries could be loaded for sequencing.Under optimized conditions:when the number of PCR cycles was 25 and the initial amount of DNA for library construction was 12.5 ng,fewer chimeras were generated.The library size was 500-700 bp,and there were no primer dimers of approximately 120 bp.This method is rapid,accurate,and sensitive,and can be used for the analysis and detection of the 16S rRNA gene of intestinal flora.
基金Administration of Traditional Chinese Medicine of Zhejiang Province and Young Talents Fund Project of Zhejiang Province Traditional Chinese Medicine Scienese and Technology Project(2022ZQ015).
文摘Background:Intratumoral flora and its metabolites play an important role in the occurrence,development and treatment of cancer,and are correlated with the genotype expression of breast cancer;However,the internal relationship between intratumoral flora and triple negative breast cancer(TNBC)has not been elucidated.Methods:Fourteen patients with TNBC who met the criteria were included.The tumor tissues and adjacent tissues were respectively taken as the patient group and the control group.The 5R 16S sequencing technique was used to detect the abundance and distribution of the intratumoral flora between the two groups,and the differences between the groups were analyzed to find the bacteria with significant differences between groups(P<0.05).Results:The abundance of intratumoral microbiota in TNBC patients was significantly lower than that in adjacent tissues(P<0.05).The differential bacteria in TNBC tumors(P<0.05)included Acinetobacter,Renibacterium,Flavobacterium,Dechloromonas and others.The differential bacteria genera(P<0.05)in the adjacent tissues included Comamonas,Bacillus,Caulobacter,Afipia,Aerococcus,Roseomonas and so on.Conclusion:There is a significant difference in the flora structure between the tumor and normal tissues in TNBC patients.Proteobacteria plays an important role in the occurrence,development and treatment of TNBC.Among them,Acinetobacter may be the key reason for the metastasis of TNBC.
基金Sponsored by National Undergraduate Innovation Training Program of Anhui Xinhua University(202412216001)Provincial-level Undergraduate Innovation Training Program of Anhui Xinhua University(S202312216041)+1 种基金Natural Science Key Research Program for Colleges and Universities in Anhui Province(2023AH051816)Anhui General Teaching Research Project(2022jyxm665).
文摘The investigation and analysis of woody plants on the campus of Anhui Xinhua University reveals the presence of 103 species distributed across 43 families and 78 genera.Notably,depauperate families and monotypic genera are found to be the most numerous.Among the families,Rosaceae is identified as the most diverse,encompassing a total of 21 species,while Prunus is found to contain the highest number of species,totaling 5.The geographic component of the plant flora is intricate and encompasses a wide range of distribution types,with a predominance of both tropic and temperate plants.
文摘AIM:To assess and compare the conjunctival bacterial flora in patients with congenital ptosis(CP)to that of healthy individuals.METHODS:The study included 38 patients with CP and 42 healthy control subjects.Conjunctival cultures were collected using a cotton-tipped swab applied to the inferior conjunctival fornix.The samples were inoculated into blood agar,eosin methylene blue agar,chocolate agar for bacteria,and Sabouraud dextrose agar medium for fungi.RESULTS:The culture positivity rate was 68.4%in CP and 47.6%in the control group(P<0.001).Additionally,there was a significant difference in culture positivity between the eyes of patients with unilateral ptosis(P=0.039).In the CP group,the most common found microorganism was Staphylococcus epidermidis at 23.67%,followed by Haemophilus species at 21.04%,and Corynebacterium species at 15.78%.In the control group,Staphylococcus epidermidis and Corynebacterium species were both the most commonly isolated microorganisms,each accounting for 19.04%.More than one species of bacteria was grown in the cultures of 36.84%and 23.80%of the CP and control subjects,respectively.Gram-negative bacteria were more common in CP than in control subjects(P=0.031).CONCLUSION:Culture positivity is significantly higher in the ptotic eyes of CP.Potentially pathogenic microorganisms are more frequently isolated from the CP group compared with the healthy control group.
基金supported by the Jilin Province Science and Technology Plan Project(20230402032GH)the Jilin Province Science and Technology Development Project(20220508115RC).
文摘The gut microbiota and it’s metabolism are vital targets of probiotics regulating high fat-diet(HFD)induced hyperlipidemia,which can relieve the pressure caused by the striking growth of sub-health people.Pediococcus pentosaceus PP04(PP04)could colonize in intestine to regulate gut microbiota and it’s metabolites directly,the rebalanced intestinal flora mediated by PP04 could facilitate the secretion of short chain fatty acids to control body weight gain,PP04 intervention also changed bile acid(BA)profiles and enhanced the ileal concentrations of antagonists including tauro-α/β-muricholic acid sodium salt and ursodeoxycholic acid to inhibit intestinal farnesoid X receptor/fibroblast growth factor 15(FXR/FGF15)signaling coupled with the activation of hepatic FXR/small heterodimer partners signaling,which accelerated the hepatic BA de novo synthesis and excretion with feces to eliminate HFD caused hyperlipemia effectively.This study provided important evidence regarding PP04 as dietary supplement to relieve hyperlipidemia by influencing BA enterohepatic circulation.
基金supported by the Global Genome Initiative for Gardens(GGI-Gardens Award),United States Botanic Garden and Botanic Gardens Conservation International,United Kingdom(Grant No.GGI/2024/1).
文摘The Andaman Islands,part of the Indo-Burma biodiversity hotspot,holds unique florawith many endemic and threatened species.However,this fragile diversity is under increasing pressure from infrastructure expansion,climate change and habitat fragmentation.The recently announced Great Nicobar Island Development project by the Government of India,which includes an international transhipment terminal,airport,township and power plant,if implemented,would cause major habitat loss and fragmentation,possibly leading to species extinctions.
基金supported by the Key Research and Development Project of Ningxia Hui Autonomous Region(2020BFG03007).
文摘Thymus quinquecostatus Celak.,a traditional aromatic edible plant from Lamiaceae,is widely used as food additive,food condiment,spice,and herbal teas.Polyphenol-rich fraction of T.quinquecostatus(PRF)has been proven to be effective protective effect for cerebral ischemia reperfusion injury(CIRI)in our previous study.In this study,we developed a novel“Gut flora-Compound-Target-Pathway”(GCTP)network based on network pharmacology coupled with gastrointestinal metabolism for screening bio-active components,key targets and gut floras through the classical technique for order preference by similarity to ideal solution(TOPSIS).This compensates for the lack of gut floras and gastrointestinal metabolism in network pharmacology.Firstly,four incubation models covering simulated gastric juice,simulated intestinal juice,gut floras of normal and transient middle cerebral artery occlusion(tMCAO)rat in vitro were applied to PRF.The 109 proto-components and 64 metabolites were elucidated by ultra-high performance liquid chromatography Q exactive orbitrap-mass spectrometry(UPLC-QE-Orbitrap-MS).Then,the key targets of matrix metalloproteinase 9(MMP9),prostaglandin-endoperoxide synthase 2(PTGS2),tyrosine-protein kinase fyn(FYN),estrogen receptor 1(ESR1),amyloid precursor protein(APP),and epidermal growth factor receptor(EGFR),and gut floras of Enterococcus avium LY1 were selected.Moreover,the selected key proto components were rosmarinic acid,daidzein,quercetin,luteolin,apigenin,methyl rosmarinate,kaempferol,luteoloside,and caffeic acid,and the key metabolites were isokaempferide,isorhamnetin,isoquercetin,and mangiferin.Binding of compounds to the key proteins was analyzed by molecular docking,and also verified though an 2,2'-azobis(2-amidinopropane)dihydrochloride(AAPH)induced oxidative stress zebrafish model and real-time quantitative polymerase chain reaction(RT-qPCR)assays.This study provides a new idea and a better understanding of PRF for its protective effects on CIRI and its underlying mechanisms.
基金This paper was supported by the Chinese Academy of Sciences (KZCX2-406) National Natural Science Foundation of China (NSFC39970123) Changbai Mountain Open Research Station.
文摘According to the investigation of sampling area of 6800 m2 on the south slope of Shennongjia Mountain, there were 126 vascular plant species, belonging to 108 genera and 64 families, in the investigated rare plant communities, of which 9 rare plant species were recorded, accounting for 27.3% of the total rare plants. The communities were about 30 m in height and were divided into three layers as tree layer, shrub layer, and herb layer. The flora of the communities had obvious temperate character. Phanerophytes (accounted for 65.9%), Mesophyllous (62.7%), Papyraceous (84.1%), simple leaf (83.3%), un-entire leaf (69.8%) were dominant in life form, leaf size class, leaf texture, leaf form, and leaf margin respectively. According to important value of species, the communities were divided into three types as Davidia involucrata + Litsea pungens community, Cercidiphyllum japanicum + Padus wilsonii community, and Padus wilsonii + Acer mono community. The indexes of species diversity of tree layer had little difference among communities and evenness was high. The results indicated that the communities had complex structure and relative stability.
文摘Based on the thorough investigation of the components of urban forest in Nanjing and the collection of samples,it is concluded that there are 1 500 species(including subspecies) of vascular plants belonging to 572 genera of 172 families,among which there are 63 species of ferns belonging to 36 genera of 25 families,1 437 species of seed plants belonging to 536 genera of 147 families.The characteristics are that the flora is rich in species,complex in geographic composition,and possess the distinct transferring quality from tropical to temperate zone.Besides,it is characterized with certain ancient nature,and the peculiar elements are comparatively simple.
文摘The tropical flora in southern China is represented mainly by three regional flora, i.e. southern Yunnan (southwestern China), southwestern Guangxi (southwestern China) and Hainan Island (southern China). The floristic composition of each is concisely enumerated. Their geographical elements at generic level are analyzed. Furthermore, a comparison of floristic similarities between southern Yunnan and Hainan Island and Malay Peninsula and Brunei of western Malesia is made, based on existing regional flora treatments. The following is concluded: (1) The flora of southern China consists mainly of tropical floristic elements which contribute about 60% at the family level and more than 80 % at the generic level of its total flora. The dominant distribution type of the flora of southern China at the generic level is the tropical Asian distribution pattern. This reveals that the flora of southern China is of tropical nature with a strong tropical Asian affinity; (2) Most of the dominant families from the flora of southern China are also dominant in the Malesian flora. The floristic similarities between the regional flora of southern China and the regional flora of western Malesia are over 70% at the family level and more than 50% at the generic level. This suggests that the tropical flora of southern China belongs to the Malesian floristic region; (3) Situated at the northern margin of tropical Asia, on the other hand it is also obvious that the flora of southern China comprises less strictly tropical elements compared to the Malesian flora, and is consequently only a marginal type of the latter; (4) The close affinity of the flora of southern China to the Malesian flora can be explained by the geological history of southeast Asia.
基金Supported by Key Project from National Spark Plan,China(2012GA820001)Special Project of Guizhou Provincial Science and Technology,China[Qiankehe Special Project(2011)6001)]+1 种基金"321"Efficient Planting Technique Integration and Demonstration of Vegetable from Technology Ombudsman,China[(2013)6061-1)]Guizhou Vegetable Industry Technique System Construction Program,China(GZCYTX2011-0101)~~
文摘To investigate the effects of different vegetable growing regions and planting modes on soil quality,soils in high,medium and low altitude areas of Guizhou were respectively sampled under different vegetable efficient planting modes,and the variations of soil microbial flora and enzyme activities were analyzed. The soil microbial count and total bacteria of the vegetable efficient cultivation mode were significantly higher than that of the control （traditional planting mode） in each planting area,and the microbial diversity index was also improved to varying de- grees.The soil phosphatase,catalase and urease activities of the vegetable efficient planting mode were higher than that of the control.The soil catalase and urease activities were higher than that of the control by 1.37-1.44 and 1.51-2.80 times. Application of vegetable efficient planting mode in different regions will help to im- prove the soil quality in a given period.
