Thirteen volatile compounds were identified from Flemingia macrophylla plants. Eight major components significantly attracted the tea green leafhoppers, Empoasca flavescens F. Based on their relative abundances, follo...Thirteen volatile compounds were identified from Flemingia macrophylla plants. Eight major components significantly attracted the tea green leafhoppers, Empoasca flavescens F. Based on their relative abundances, following synthetic blends were made for field experiments: 1) eight-component-attractant blend included Z-3-hexen-1-ol, Z-3-hexenyl acetate, Z-ocimene, Me SA, Z-3-hexenyl butyrate, dodecane, hexadecane and nonanal at 10, 10, 1, 11, 2, 6, 2 and 4 mg mL^-1 in n-hexane, respectively;2) four-component-attractant blend #1 contained hexadecane, Z-3-hexenyl acetate, Z-3-hexen-1-ol and nonanal at 2, 10, 10 and 4 mg mL^-1 in n-hexane, respectively;3) four-component-attractant blend #2 contained hexadecane, Z-3-hexenyl acetate, Z-3-hexen-1-ol and Me SA at 2, 10, 10 and 11 mg mL^-1 in n-hexane, respectively. Thymol and 1-methoxy-4-methyl-2-(1-methylethyl)-benzene, identified from Lavandula angustifolia aeration samples, significantly repelled the leafhopper as strong repellents when tested alone or in combination at 10 mg mL^-1. For field bioassays, each attractant lure was attached to a bud green sticky board hung from a bamboo stick at above tea plant level for catching the leafhoppers, whereas the repellent dispenser was tied to a tea branch inside tea clump for pushing the leafhoppers away from tea clumps. The results showed that the eight-component-attractant blend caught similar numbers of the leafhopper as did the four-component-attractant blend #1 at about 53–79 leafhoppers/trap/day, which were significantly higher than those on the hexane-control bud green sticky boards. Average leafhopper catches from un-baited sticky boards were about 51–73 leafhoppers/trap/day when pushed by the repellents placed inside tea plants, with the two-component-repellent blend being more effective than their single components. When the two-component-repellent blend was further tested with the three attractant blends in a push-pull fashion, average trap catches ranged from 62 to 92 leafhoppers/trap/day. Control efficacy on the leafhoppers within the push-pull zones increased progressively from day 1(43%) to day 5(73%). This push-pull approach might have a great potential as a green control strategy for combating the tea green leafhoppers.展开更多
Four hitherto unknown polysubstituted isofiavonoids,including three isofiavans:7,4'-dihydroxy-8,2',3'-trimethoxyisofiavan(1),7,2',4'-trihydroxy-8,3'-dimethoxyisofiavan(2),and 7,2',4'-tr...Four hitherto unknown polysubstituted isofiavonoids,including three isofiavans:7,4'-dihydroxy-8,2',3'-trimethoxyisofiavan(1),7,2',4'-trihydroxy-8,3'-dimethoxyisofiavan(2),and 7,2',4'-trihydroxy-5-methoxyisofiavan(3),and one prenylated isofiavone cudraisofiavone M(4)were isolated from the ethanol extracts of Spatholobus suberectus(for 1 and 2),Flemingia macrophylla(for 3),and Cudrania cochinchinensis(for 4),respectively.Their structures were established on the basis of extensive spectroscopic analysis.Compounds 1 and 4 exhibited weak cytotoxic activity against five human cancer cell lines(HL-60,A-549,SMMC-7721,MCF-7,and SW-480).展开更多
Objective:To investigate the effect and anti-inflammatory mechanism the effect of Flemingia Roxb.exAit extract on acute gouty arthritis.Methods:Sixty SD rats were randomly divided into 6 groups by weight,control and m...Objective:To investigate the effect and anti-inflammatory mechanism the effect of Flemingia Roxb.exAit extract on acute gouty arthritis.Methods:Sixty SD rats were randomly divided into 6 groups by weight,control and model group,(100、150、200 mg/kg)of Flemingia Roxb.exAit extract group and colchicine group.The rats of each group were given by intragastric for 7 days of continuous administration,meanwhile,there were given 0.9%NaCl solution instead as control and model group.The acute gouty arthritis model was constructed through right ankle joint cavity injection of sodium urate crystal solutionon day 5 after 1 h,but rats in the negative control group were injected with 0.9%NaCl solution into the articular cavity of the right foot.The general condition of rats and the degree of joint swelling,and the gait was observed after constructed model.To detected the joint fluid IL-1β、TNF-α、IL-6 indicators,and the expression of NLRP3,Caspase-1 protein in ankle joint tissue for 2 hours after the last administration.Results:Groups of Flemingia Roxb.exAit extract could markedly reduce the joint swelling on acute gouty arthritis rat model,improve gait in a dose-dependent manner,high dose group of Flemingia Roxb.exAit extract did particularly well(Pjoint swelling<0.01;Pgait<0.05),close to the therapeutic effect colchicine group.Further mechanism studies show that the joint fluid IL-1β、TNF-α、IL-6 level was reduced by Flemingia Roxb.exAit extract group,the expressions of NLRP3、Caspase-1 protein of Flemingia Roxb.exAit extract group was decreased observbly than model group,but the most obvious is that high dose group as well as colchicine group(PNALP3<0.01;PCaspase-1<0.01).Conclusion:Flemingia Roxb.exAit extract has the effect of treating acute gouty arthritis and its mechanism may be related toinhibiting NLRP3 inflammasome activation and inhibiting inflammatory response.展开更多
Objective To study the chemical constituents from the roots of Flemingia philippinensis. Methods The chemical constituents were isolated and purified by combination of silica gel column, Sephadex LH-20, polyamide, and...Objective To study the chemical constituents from the roots of Flemingia philippinensis. Methods The chemical constituents were isolated and purified by combination of silica gel column, Sephadex LH-20, polyamide, and ODS column chromatography. The structures of the isolated compounds were identified by means of spectral data. Results Ten compounds were isolated from F. philippinensis and identified as isoderrone (1), dalparvin A (2), prunetin (3), 7,3′-dihydroxy-5,4′,5′-trimethoxyisoflavone (4), pratensein-7-O-β-D-glucoside (5), sissotrin (6), sophororicoside (7), formononetin (8), orobol (9), and biochanin A (10). Conclusion Compounds 1-6 are obtained from this plant for the first time.展开更多
基金financially supported by the National Key Research and Development Program of China (2018YFC1604402)the Natural Science Foundation of Zhejiang Province, China (LY17C140002)+1 种基金the Fundamental and Public Welfare of Zhejiang Province, China (LGN18C160006)the College Student Innovation and Entrepreneurship of Zhejiang Province, China (2017R409055)
文摘Thirteen volatile compounds were identified from Flemingia macrophylla plants. Eight major components significantly attracted the tea green leafhoppers, Empoasca flavescens F. Based on their relative abundances, following synthetic blends were made for field experiments: 1) eight-component-attractant blend included Z-3-hexen-1-ol, Z-3-hexenyl acetate, Z-ocimene, Me SA, Z-3-hexenyl butyrate, dodecane, hexadecane and nonanal at 10, 10, 1, 11, 2, 6, 2 and 4 mg mL^-1 in n-hexane, respectively;2) four-component-attractant blend #1 contained hexadecane, Z-3-hexenyl acetate, Z-3-hexen-1-ol and nonanal at 2, 10, 10 and 4 mg mL^-1 in n-hexane, respectively;3) four-component-attractant blend #2 contained hexadecane, Z-3-hexenyl acetate, Z-3-hexen-1-ol and Me SA at 2, 10, 10 and 11 mg mL^-1 in n-hexane, respectively. Thymol and 1-methoxy-4-methyl-2-(1-methylethyl)-benzene, identified from Lavandula angustifolia aeration samples, significantly repelled the leafhopper as strong repellents when tested alone or in combination at 10 mg mL^-1. For field bioassays, each attractant lure was attached to a bud green sticky board hung from a bamboo stick at above tea plant level for catching the leafhoppers, whereas the repellent dispenser was tied to a tea branch inside tea clump for pushing the leafhoppers away from tea clumps. The results showed that the eight-component-attractant blend caught similar numbers of the leafhopper as did the four-component-attractant blend #1 at about 53–79 leafhoppers/trap/day, which were significantly higher than those on the hexane-control bud green sticky boards. Average leafhopper catches from un-baited sticky boards were about 51–73 leafhoppers/trap/day when pushed by the repellents placed inside tea plants, with the two-component-repellent blend being more effective than their single components. When the two-component-repellent blend was further tested with the three attractant blends in a push-pull fashion, average trap catches ranged from 62 to 92 leafhoppers/trap/day. Control efficacy on the leafhoppers within the push-pull zones increased progressively from day 1(43%) to day 5(73%). This push-pull approach might have a great potential as a green control strategy for combating the tea green leafhoppers.
基金supported by the"Large-scale Compound Library"project of National Development and Reform Commission of China.
文摘Four hitherto unknown polysubstituted isofiavonoids,including three isofiavans:7,4'-dihydroxy-8,2',3'-trimethoxyisofiavan(1),7,2',4'-trihydroxy-8,3'-dimethoxyisofiavan(2),and 7,2',4'-trihydroxy-5-methoxyisofiavan(3),and one prenylated isofiavone cudraisofiavone M(4)were isolated from the ethanol extracts of Spatholobus suberectus(for 1 and 2),Flemingia macrophylla(for 3),and Cudrania cochinchinensis(for 4),respectively.Their structures were established on the basis of extensive spectroscopic analysis.Compounds 1 and 4 exhibited weak cytotoxic activity against five human cancer cell lines(HL-60,A-549,SMMC-7721,MCF-7,and SW-480).
基金2016 Hainan Medical College Students Innovation and Entrepreneurship Training Program(No.HYCX2016055)2017 Hainan College Students Innovation and Entrepreneurship Training Program Project(No.2017098)。
文摘Objective:To investigate the effect and anti-inflammatory mechanism the effect of Flemingia Roxb.exAit extract on acute gouty arthritis.Methods:Sixty SD rats were randomly divided into 6 groups by weight,control and model group,(100、150、200 mg/kg)of Flemingia Roxb.exAit extract group and colchicine group.The rats of each group were given by intragastric for 7 days of continuous administration,meanwhile,there were given 0.9%NaCl solution instead as control and model group.The acute gouty arthritis model was constructed through right ankle joint cavity injection of sodium urate crystal solutionon day 5 after 1 h,but rats in the negative control group were injected with 0.9%NaCl solution into the articular cavity of the right foot.The general condition of rats and the degree of joint swelling,and the gait was observed after constructed model.To detected the joint fluid IL-1β、TNF-α、IL-6 indicators,and the expression of NLRP3,Caspase-1 protein in ankle joint tissue for 2 hours after the last administration.Results:Groups of Flemingia Roxb.exAit extract could markedly reduce the joint swelling on acute gouty arthritis rat model,improve gait in a dose-dependent manner,high dose group of Flemingia Roxb.exAit extract did particularly well(Pjoint swelling<0.01;Pgait<0.05),close to the therapeutic effect colchicine group.Further mechanism studies show that the joint fluid IL-1β、TNF-α、IL-6 level was reduced by Flemingia Roxb.exAit extract group,the expressions of NLRP3、Caspase-1 protein of Flemingia Roxb.exAit extract group was decreased observbly than model group,but the most obvious is that high dose group as well as colchicine group(PNALP3<0.01;PCaspase-1<0.01).Conclusion:Flemingia Roxb.exAit extract has the effect of treating acute gouty arthritis and its mechanism may be related toinhibiting NLRP3 inflammasome activation and inhibiting inflammatory response.
基金National Natural Science Foundation of China (30973635)the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-YW-R-217+2 种基金 KSCX2-EW-J-28)the Program of South China Botanical Garden (201037)Guangdong Province Natural Science Foundation (10151065005000026)
文摘Objective To study the chemical constituents from the roots of Flemingia philippinensis. Methods The chemical constituents were isolated and purified by combination of silica gel column, Sephadex LH-20, polyamide, and ODS column chromatography. The structures of the isolated compounds were identified by means of spectral data. Results Ten compounds were isolated from F. philippinensis and identified as isoderrone (1), dalparvin A (2), prunetin (3), 7,3′-dihydroxy-5,4′,5′-trimethoxyisoflavone (4), pratensein-7-O-β-D-glucoside (5), sissotrin (6), sophororicoside (7), formononetin (8), orobol (9), and biochanin A (10). Conclusion Compounds 1-6 are obtained from this plant for the first time.
