Adenosma buchneroides Bonati,also known as fleagrass,is an important medicinal plant used by the Akha(Hani)people of China for treating inflammation-related skin swelling,acne,and diarrhoea,among other conditions.In t...Adenosma buchneroides Bonati,also known as fleagrass,is an important medicinal plant used by the Akha(Hani)people of China for treating inflammation-related skin swelling,acne,and diarrhoea,among other conditions.In this study,we aimed to evaluate the anti-inflammatory activities and explore the molecular mechanisms of fleagrass on treating skin swelling and acne.The results demonstrated that fleagrass inhibited the enzymatic activities of 5-LOX and COX-2 in vitro,and decreased the release of NO,IL-6,TNF-α,and IL-10 in the LPS-induced RAW264.7 macrophages.The levels of proteins associated with the nuclear factor-kappa B(NF-κB)pathway were examined by western blotting and immunofluorescence,demonstrating that fleagrass downregulated the expression of TLR4,MyD88,NF-κB/p65,and iNOS and blocked the nuclear translocation of NF-κB/p65.Furthermore,fleagrass exhibited acute anti-inflammatory activity in paw oedema models.The results confirm that fleagrass exhibits remarkable anti-inflammatory activity and can be used in alleviating inflammation,suggesting that fleagrass has the potential to be a novel anti-inflammatory agent.展开更多
基金funded by Plant Germplasm Resources Innovation Project of Chinese Academy of Sciences(No.KFJ-BRP-007-002)Yunnan Revitalization Talent Support Program Industrial Innovation Talent Project(Fleagrass Planting and Mosquito Repellent Product Industrialization Research and Development).
文摘Adenosma buchneroides Bonati,also known as fleagrass,is an important medicinal plant used by the Akha(Hani)people of China for treating inflammation-related skin swelling,acne,and diarrhoea,among other conditions.In this study,we aimed to evaluate the anti-inflammatory activities and explore the molecular mechanisms of fleagrass on treating skin swelling and acne.The results demonstrated that fleagrass inhibited the enzymatic activities of 5-LOX and COX-2 in vitro,and decreased the release of NO,IL-6,TNF-α,and IL-10 in the LPS-induced RAW264.7 macrophages.The levels of proteins associated with the nuclear factor-kappa B(NF-κB)pathway were examined by western blotting and immunofluorescence,demonstrating that fleagrass downregulated the expression of TLR4,MyD88,NF-κB/p65,and iNOS and blocked the nuclear translocation of NF-κB/p65.Furthermore,fleagrass exhibited acute anti-inflammatory activity in paw oedema models.The results confirm that fleagrass exhibits remarkable anti-inflammatory activity and can be used in alleviating inflammation,suggesting that fleagrass has the potential to be a novel anti-inflammatory agent.
