Objective: To evaluate the scientific basis for the use of Kang 601 heji(K-601) as an anti-in flammatory and antipyretic agent using appropriate animal models.Methods: Carrageenan-induced rat paw and xylene-induced ea...Objective: To evaluate the scientific basis for the use of Kang 601 heji(K-601) as an anti-in flammatory and antipyretic agent using appropriate animal models.Methods: Carrageenan-induced rat paw and xylene-induced ear oedemas were models used to investigate anti-in flammatory actions of K-601. Lipopolysaccharide-induced pyrexia model was used to evaluate antipyretic activity in Wistar rats. The antiin flammatory and antipyretic mechanisms were evaluated by detecting prostaglandins E2, nitric oxide, interleukin-1b and tumour necrosis factora levels using appropriate reagents and ELISA kits.Results: The results revealed that K-601 reduced the level of in flammations in both antiin flammatory models in a dose-dependent manner. The same was true for the antipyretic model. The possible mechanisms of actions were through the inhibition of prostaglandins E2, interleukin-1b, tumour necrosis factora and nitric oxide.Conclusions: K-601 has proven anti-in flammatory and antipyretic actions. The findings provide a scientific basis for the use of K-601 as anti-in flammatory and antipyretic agent in traditional Chinese medicinal practice.展开更多
Objective:To evaluate the effect of Mycoplasma pneumoniae pneumonia (MPP) on inflammatory factors.Methods:Using the combination of subject terms and free words to search the literature on the effect of children with M...Objective:To evaluate the effect of Mycoplasma pneumoniae pneumonia (MPP) on inflammatory factors.Methods:Using the combination of subject terms and free words to search the literature on the effect of children with MPP on inflammatory factors.Retrieved from CNKI, VIP,CBM, Wanfang Database, Pubmed Database, Sciencedirect Database and Google scholor. etc.Time period of retrieval was from database established to May. 2019. 2 researchers conducted literature screening independently according to the inclusion and exclusion criteria. and then processed quality evaluation.RevMan5.3 and stata 14.0 were adopted to conduct Meta analysis on all effective indicators. Results:A total of 23 studies were involved, including 2254 children with MPP and 1182 healthy children. Results of Meta analysis showed IL-6 level(SMD=17.74, 95% CI =15.38~20.09,P<0.0001)、IL-8 level (SMD=19.40,95% CI=18.12~20.69,P<0.0001)、IL-10 level (SMD=9.51,95% CI =8.25~10.97,P<0.0001)、TNF-a level (SMD=31.06,95% CI =24.57~37.54,P<0.0001) were significantly better than the control group, the difference were statistically significant. Subgroup analysis:SMPP compared with MPP, IL-6 level (SMD=12.02, 95% CI =7.08~16.96,P<0.0001)was significantly better than the control group, the difference were statistically significant;Compared with the recovery period, the acute phase of children with MPP, IL-6 level (SMD=20.53, 95% CI =17.51~23.56,P<0.0001)、IL-8 level (SMD=17.27,95% CI=10.14~24.40,P<0.0001)、IL-10 level (SMD=6.04,95% CI =1.72~10.36,P<0.0001)、TNF-a level (SMD=23.64,95% CI =17.60~29.69,P<0.0001) were significantly better than the control group, the difference was statistically significant. Conclusions:MPP caused elevated levels of IL-6, IL-8, IL-10, and TNF-a inflammatory factors in the serum of children, and the levels of inflammatory factors were directly proportional to the severity of the disease.展开更多
Isoflavone formononetin（FN） is a main active component of red clover（Trifolium pratense L.）, a medicinal plant possessing antitumorigenic and antioxidant properties. In the present study, we aimed to examine the e...Isoflavone formononetin（FN） is a main active component of red clover（Trifolium pratense L.）, a medicinal plant possessing antitumorigenic and antioxidant properties. In the present study, we aimed to examine the effect of FN on dextran sulfate sodium（DSS）-induced ulcerative colitis（UC） in mice. The results showed that FN（25, 50 mg/kg） markedly attenuated the loss of body weight, the disease activity index（DAI）, shortening of colon length and tissue injury induced by DSS treatment. In addition, the levels of tumor necrosis factor-α（TNF-α）, interleukin-6（IL-6）, and cyclooxygenase-2（COX-2） were also significantly reduced in FN treatment group compared with the DSS group. Moreover, several representative oxidative stress parameters in colorectum, including superoxide dismutase（SOD）, methane dicarboxylic aldehyde（MDA）, myeloperoxidase（MPO） and 8-oxoguanine, were markedly ameliorated. In this study, we also found that the expression of Nrf2 was increased by FN treatment. However, symptoms of UC were not ameliorated in Nrf2 knockout mice. Taken together, FN could prevent the development of UC through activating of Nrf2 axis, and the protective effect was Nrf2 dependent. Our results demonstrated that FN might be a potential therapeutic agent in the treatment of UC.展开更多
基金Supported in part by the National Natural Science Foundation of China(Nos.81222052 and 81421005)Jiangsu Province Science Fund for Distinguished Young Scholars(BK20130025)
文摘Objective: To evaluate the scientific basis for the use of Kang 601 heji(K-601) as an anti-in flammatory and antipyretic agent using appropriate animal models.Methods: Carrageenan-induced rat paw and xylene-induced ear oedemas were models used to investigate anti-in flammatory actions of K-601. Lipopolysaccharide-induced pyrexia model was used to evaluate antipyretic activity in Wistar rats. The antiin flammatory and antipyretic mechanisms were evaluated by detecting prostaglandins E2, nitric oxide, interleukin-1b and tumour necrosis factora levels using appropriate reagents and ELISA kits.Results: The results revealed that K-601 reduced the level of in flammations in both antiin flammatory models in a dose-dependent manner. The same was true for the antipyretic model. The possible mechanisms of actions were through the inhibition of prostaglandins E2, interleukin-1b, tumour necrosis factora and nitric oxide.Conclusions: K-601 has proven anti-in flammatory and antipyretic actions. The findings provide a scientific basis for the use of K-601 as anti-in flammatory and antipyretic agent in traditional Chinese medicinal practice.
基金NSFC(No.81373687、81874490)Liaoning province"hundreds of thousands of talents project"candidate funding project(No.[2018]47)Shenyang science and technology innovation program for young and middle-aged talent(No.RC180246).
文摘Objective:To evaluate the effect of Mycoplasma pneumoniae pneumonia (MPP) on inflammatory factors.Methods:Using the combination of subject terms and free words to search the literature on the effect of children with MPP on inflammatory factors.Retrieved from CNKI, VIP,CBM, Wanfang Database, Pubmed Database, Sciencedirect Database and Google scholor. etc.Time period of retrieval was from database established to May. 2019. 2 researchers conducted literature screening independently according to the inclusion and exclusion criteria. and then processed quality evaluation.RevMan5.3 and stata 14.0 were adopted to conduct Meta analysis on all effective indicators. Results:A total of 23 studies were involved, including 2254 children with MPP and 1182 healthy children. Results of Meta analysis showed IL-6 level(SMD=17.74, 95% CI =15.38~20.09,P<0.0001)、IL-8 level (SMD=19.40,95% CI=18.12~20.69,P<0.0001)、IL-10 level (SMD=9.51,95% CI =8.25~10.97,P<0.0001)、TNF-a level (SMD=31.06,95% CI =24.57~37.54,P<0.0001) were significantly better than the control group, the difference were statistically significant. Subgroup analysis:SMPP compared with MPP, IL-6 level (SMD=12.02, 95% CI =7.08~16.96,P<0.0001)was significantly better than the control group, the difference were statistically significant;Compared with the recovery period, the acute phase of children with MPP, IL-6 level (SMD=20.53, 95% CI =17.51~23.56,P<0.0001)、IL-8 level (SMD=17.27,95% CI=10.14~24.40,P<0.0001)、IL-10 level (SMD=6.04,95% CI =1.72~10.36,P<0.0001)、TNF-a level (SMD=23.64,95% CI =17.60~29.69,P<0.0001) were significantly better than the control group, the difference was statistically significant. Conclusions:MPP caused elevated levels of IL-6, IL-8, IL-10, and TNF-a inflammatory factors in the serum of children, and the levels of inflammatory factors were directly proportional to the severity of the disease.
基金National Natural Science Foundation of China(Grant No.81274150,81573680 and 81470179)
文摘Isoflavone formononetin（FN） is a main active component of red clover（Trifolium pratense L.）, a medicinal plant possessing antitumorigenic and antioxidant properties. In the present study, we aimed to examine the effect of FN on dextran sulfate sodium（DSS）-induced ulcerative colitis（UC） in mice. The results showed that FN（25, 50 mg/kg） markedly attenuated the loss of body weight, the disease activity index（DAI）, shortening of colon length and tissue injury induced by DSS treatment. In addition, the levels of tumor necrosis factor-α（TNF-α）, interleukin-6（IL-6）, and cyclooxygenase-2（COX-2） were also significantly reduced in FN treatment group compared with the DSS group. Moreover, several representative oxidative stress parameters in colorectum, including superoxide dismutase（SOD）, methane dicarboxylic aldehyde（MDA）, myeloperoxidase（MPO） and 8-oxoguanine, were markedly ameliorated. In this study, we also found that the expression of Nrf2 was increased by FN treatment. However, symptoms of UC were not ameliorated in Nrf2 knockout mice. Taken together, FN could prevent the development of UC through activating of Nrf2 axis, and the protective effect was Nrf2 dependent. Our results demonstrated that FN might be a potential therapeutic agent in the treatment of UC.
