The width of rice leaves determines the size of the photosynthetic area.Optimizing rice leaf width can improve the photosynthetic rate,thereby increasing rice yield.In this study,a genome-wide association study(GWAS)w...The width of rice leaves determines the size of the photosynthetic area.Optimizing rice leaf width can improve the photosynthetic rate,thereby increasing rice yield.In this study,a genome-wide association study(GWAS)was conducted by 225 rice germplasm resources to explore the genetic basis of rice flag leaf width(FLW).We identified nine QTLs associated with FLW(qFLWs),with phenotypic contribution rates ranging from 3.17%to 14.37%.Near-isogenic lines(NILs)were developed for fine-mapping of qFLW11,and the function of FLW11 was further verified.We narrowed down q FLW11 to an 87-kb interval,which contains five genes.展开更多
基金supported by the Zhejiang Provincial Natural Science Foundation,China(Grant No.LD24C130001)the National Natural Science Foundation of China(Grant Nos.W2412006 and 32372125)+3 种基金the Hainan Provincial Natural Science Foundation,China(Grant Nos.GHYF2025029 and YBXM2422)the Innovation Platform for Academicians of Hainan Province,China(Grant No.YSPTZX202502)the National Modern Agricultural Industry Technology System Project,China(Grant No.CARS-01-18)the Special Support Program of Chinese Academy of Agricultural Sciences(Grant Nos.NKYCLJ-C-2021-015 and CAAS-ZDRW202401)。
文摘The width of rice leaves determines the size of the photosynthetic area.Optimizing rice leaf width can improve the photosynthetic rate,thereby increasing rice yield.In this study,a genome-wide association study(GWAS)was conducted by 225 rice germplasm resources to explore the genetic basis of rice flag leaf width(FLW).We identified nine QTLs associated with FLW(qFLWs),with phenotypic contribution rates ranging from 3.17%to 14.37%.Near-isogenic lines(NILs)were developed for fine-mapping of qFLW11,and the function of FLW11 was further verified.We narrowed down q FLW11 to an 87-kb interval,which contains five genes.
文摘叶片是植物进行光合作用的主要器官,与植物形态建成有重要关系。旗叶的大小及其与茎杆的夹角直接影响到小麦植株的受光,从而影响到小麦的产量水平。本研究利用比较基因组学的方法,以水稻重要叶宽基因Os NAL1为参考序列在小麦中克隆其同源基因TaNAL1-5,挖掘其优良等位变异并研究其对旗叶宽等重要农艺性状的影响。结果表明,小麦TaNAL1-5A、TaNAL1-5B和TaNAL1-5D基因均由5个外显子和4个内含子组成,TaNAL1-5A和TaNAL1-5B编码600个氨基酸,TaNAL1-5D编码598个氨基酸,具有典型的半胱氨酸/丝氨酸胰蛋白酶结构域。系统进化树分析发现小麦TaNAL1-5基因与乌拉尔图小麦、粗山羊草、二穗短柄草等单子叶植物亲缘关系较近,而与拟南芥、大豆等双子叶植物亲缘关系较远。在不同小麦品种中,TaNAL1-5B/5D没有检测到序列多态性,而TaNAL1-5A呈现单体型的9个SNP位点和一个19 bp Indel的两种等位变异,并根据19 bp的Indel设计了TaNAL1-5A两种等位变异的功能标记。功能标记扫描发现,TaNAL1-5A与小麦旗叶宽度、千粒重、小穗数呈极显著正相关。此外,基因表达分析显示TaNAL1-5基因在籽粒、根、茎、叶、穗、穗下节等部位均表达,且在开花后25天即灌浆高峰期表达量最高,可能参与了籽粒的形成过程。本研究有助于解析小麦旗叶形成的遗传控制及株型改良。
