目的：测定贵阳地区正常人群长波紫外线（UVA）、中波紫外线（UVB）的最小红斑量（MED）。方法：以SUV-l000型日光紫外线模拟器作为照射光源,测定190例健康志愿者腹部正常皮肤UVA-MED及UVB-MED,比较不同皮肤类型、性别、年龄及季节受试...目的：测定贵阳地区正常人群长波紫外线（UVA）、中波紫外线（UVB）的最小红斑量（MED）。方法：以SUV-l000型日光紫外线模拟器作为照射光源,测定190例健康志愿者腹部正常皮肤UVA-MED及UVB-MED,比较不同皮肤类型、性别、年龄及季节受试者UVA-MED及UVB-MED。结果：190例志愿者UVA-MED中位数为48.25 J/cm2,UVB-MED为35.24 m J/cm2,男性UVA-MED和UVB-MED中位数均高于女性（P〈0.01）,Ⅳ型皮肤MED中位数亦均显著高于Ⅲ型皮肤（P〈0.01）;男性10～29岁组及30～49岁组UVA-MED明显高于≥50岁组（P〈0.01）,10～29岁组UVB-MED高于30～49岁组及≥50岁组（P〈0.05）;男性夏季UVA-MED显著低于春、秋两季（P〈0.01）,女性夏季UVA-MED亦显著低于春、冬两季（P〈0.01）;男、女性夏季UVB-MED低于春、秋、冬季（P〈0.05）。结论：皮肤日光反应类型、性别、年龄及季节是影响贵阳地区人群MED的重要因素。展开更多
Background:Wound healing can result in various outcomes,including hypertrophic scar(HTS).Pigs serve as models to study wound healing as their skin shares physi-ologic similarity with humans.Yorkshire(Yk)and Duroc(Dc)p...Background:Wound healing can result in various outcomes,including hypertrophic scar(HTS).Pigs serve as models to study wound healing as their skin shares physi-ologic similarity with humans.Yorkshire(Yk)and Duroc(Dc)pigs have been used to mimic normal and abnormal wound healing,respectively.The reason behind this differential healing phenotype was explored here.Methods:Excisional wounds were made on Dc and Yk pigs and were sampled and imaged for 98 days.PCR arrays were used to determine differential gene expression.Vancouver Scar Scale(VSS)scores were given.Re-epithelialization was analyzed.H&E,Mason's trichrome,and immunostains were used to determine cellularity,col-lagen content,and blood vessel density,respectively.Results:Yk wounds heal to a“port wine”HTS,resembling scarring in Fitzpatrick skin types(FST)I-III.Dc wounds heal to a dyspigmented,non-pliable HTS,resembling scarring in FST IV-VI.Gene expression during wound healing was differentially regulated versus uninjured skin in 40/80 genes,15 of which differed between breeds.Yk scars had a higher VSS score at all time points.Yk and Dc wounds had equivalent re-epithelialization,collagen disorganization,and blood vessel density.Conclusions:Our findings demonstrate that Dc and Yk pigs can produce HTS.Wound creation and healing were consistent among breeds,and differences in gene expression were not sufficient to explain differences in resulting scar phenotype.Both pig breeds should be used in animal models to investigate novel therapeutics to provide insight into a treatment's effectiveness on various skin types.展开更多
文摘目的：测定贵阳地区正常人群长波紫外线（UVA）、中波紫外线（UVB）的最小红斑量（MED）。方法：以SUV-l000型日光紫外线模拟器作为照射光源,测定190例健康志愿者腹部正常皮肤UVA-MED及UVB-MED,比较不同皮肤类型、性别、年龄及季节受试者UVA-MED及UVB-MED。结果：190例志愿者UVA-MED中位数为48.25 J/cm2,UVB-MED为35.24 m J/cm2,男性UVA-MED和UVB-MED中位数均高于女性（P〈0.01）,Ⅳ型皮肤MED中位数亦均显著高于Ⅲ型皮肤（P〈0.01）;男性10～29岁组及30～49岁组UVA-MED明显高于≥50岁组（P〈0.01）,10～29岁组UVB-MED高于30～49岁组及≥50岁组（P〈0.05）;男性夏季UVA-MED显著低于春、秋两季（P〈0.01）,女性夏季UVA-MED亦显著低于春、冬两季（P〈0.01）;男、女性夏季UVB-MED低于春、秋、冬季（P〈0.05）。结论：皮肤日光反应类型、性别、年龄及季节是影响贵阳地区人群MED的重要因素。
基金This work was funded in part by R01 AR070851-01A1.
文摘Background:Wound healing can result in various outcomes,including hypertrophic scar(HTS).Pigs serve as models to study wound healing as their skin shares physi-ologic similarity with humans.Yorkshire(Yk)and Duroc(Dc)pigs have been used to mimic normal and abnormal wound healing,respectively.The reason behind this differential healing phenotype was explored here.Methods:Excisional wounds were made on Dc and Yk pigs and were sampled and imaged for 98 days.PCR arrays were used to determine differential gene expression.Vancouver Scar Scale(VSS)scores were given.Re-epithelialization was analyzed.H&E,Mason's trichrome,and immunostains were used to determine cellularity,col-lagen content,and blood vessel density,respectively.Results:Yk wounds heal to a“port wine”HTS,resembling scarring in Fitzpatrick skin types(FST)I-III.Dc wounds heal to a dyspigmented,non-pliable HTS,resembling scarring in FST IV-VI.Gene expression during wound healing was differentially regulated versus uninjured skin in 40/80 genes,15 of which differed between breeds.Yk scars had a higher VSS score at all time points.Yk and Dc wounds had equivalent re-epithelialization,collagen disorganization,and blood vessel density.Conclusions:Our findings demonstrate that Dc and Yk pigs can produce HTS.Wound creation and healing were consistent among breeds,and differences in gene expression were not sufficient to explain differences in resulting scar phenotype.Both pig breeds should be used in animal models to investigate novel therapeutics to provide insight into a treatment's effectiveness on various skin types.
