Studying immiscible fluid displacement patterns can provide a better understanding of displacement processes within heterogeneous porous media,thereby helping improving oil recovery and optimizing geological CO_(2) se...Studying immiscible fluid displacement patterns can provide a better understanding of displacement processes within heterogeneous porous media,thereby helping improving oil recovery and optimizing geological CO_(2) sequestration.As the injection rate of water displacing oil increases and the displacement pattern transits from capillary fingering to viscous fingering,there is a broad crossover zone between the two that can adversely affect the oil displacement efficiency.While previous studies have utilized phase diagrams to investigate the influence of the viscosity ratio and wettability of the crossover zone,fewer have studied the impact of rock heterogeneity.In this study,we created pore network models with varying degrees of heterogeneity to simulate water flooding at different injection rates.Our model quantifies capillary and viscous fingering characteristics while investigating porous media heterogeneity's role in the crossover zone.Analysis of simulation results reveals that a higher characteristic front flow rate within the crossover zone leads to earlier breakthrough and reduced displacement efficiency.Increased heterogeneity in the porous media raises injection-site pressure,lowers water saturation,and elevates the characteristic front flow rate,thereby expanding the extent of crossover zone.展开更多
Viscous fingering is one of the main challenges that could reduce areal sweep efficiency during waterflooding in oil reservoirs. A series of waterflooding experiments were carried out in a Hele-Shaw cell at ambient te...Viscous fingering is one of the main challenges that could reduce areal sweep efficiency during waterflooding in oil reservoirs. A series of waterflooding experiments were carried out in a Hele-Shaw cell at ambient temperature during which areal sweep efficiency was estimated and techniques to ease the fingering problem were examined. The onset and propagation of viscous fingers were monitored as a function of both injection rate and injection/production positions. Image processing techniques were utilized to quantitatively investigate the propagation of fingers. The experimental results show that, under specific conditions, increasing the number of finger branches could improve the areal sweep efficiency, whereas growth of a single narrow finger has a negative impact on oil displacement efficiency. According to the obtained results,increasing the injection rate improves the areal sweep efficiency up to a critical rate at which viscous fingers start to grow.The impact of heterogeneity of the medium on distributing the viscous fingers was also investigated by introducing two different arrangements of fractures in the model. The results show that fractures perpendicular to the direction of flow would distribute the displacing water more uniformly, while fractures in the direction of flow would amplify the unfavorable sweep efficiency.展开更多
Fingering phenomena are common occurrence in the natural world. It generally takes place when a less viscous fluid displacesa more viscous fluid typically in porous media. Nowadays, such phenomena have extensively bee...Fingering phenomena are common occurrence in the natural world. It generally takes place when a less viscous fluid displacesa more viscous fluid typically in porous media. Nowadays, such phenomena have extensively been studied due to itsimportance in many industrial fields. In this paper, the effects of surface wettability on finger pattern are studied and simulatednumerically by the Lattice Boltzmann method (LBM). The displacement efficiency is investigated by using two parameters,namely, the breakthrough time and the areal sweep efficiency. The simulation has demonstrated that surface wettability willinfluence the finger pattern no matter the gravity is considered or not, but in the presence of gravity, the finger pattern is muchmore complicated and irregular due to the coexistence and competition of capillary force, viscous force and gravity.展开更多
Understanding fingering, as a challenge to stable displacement during the immiscible flow, has become a crucial phenomenon for geological carbon sequestration, enhanced oil recovery, and groundwater protection. Typica...Understanding fingering, as a challenge to stable displacement during the immiscible flow, has become a crucial phenomenon for geological carbon sequestration, enhanced oil recovery, and groundwater protection. Typically governed by gravity, viscous and capillary forces, these factors lead invasive fluids to occupy pore space irregularly and incompletely. Previous studies have demonstrated capillary numbers,describing the viscous and capillary forces, to quantificationally induce evolution of invasion patterns.While the evolution mechanisms of invasive patterns have not been deeply elucidated under the constant capillary number and three variable parameters including velocity, viscosity, and interfacial tension.Our research employs two horizontal visualization systems and a two-phase laminar flow simulation to investigate the tendency of invasive pattern transition by various parameters at the pore scale. We showed that increasing invasive viscosity or reducing interfacial tension in a homogeneous pore space significantly enhanced sweep efficiency, under constant capillary number. Additionally, in the fingering crossover pattern, the region near the inlet was prone to capillary fingering with multi-directional invasion, while the viscous fingering with unidirectional invasion was more susceptible occurred in the region near the outlet. Furthermore, increasing invasive viscosity or decreasing invasive velocity and interfacial tension promoted the extension of viscous fingering from the outlet to the inlet, presenting that the subsequent invasive fluid flows toward the outlet. In the case of invasive trunk along a unidirectional path, the invasive flow increased exponentially closer to the outlet, resulting in a significant decrease in the width of the invasive interface. Our work holds promising applications for optimizing invasive patterns in heterogeneous porous media.展开更多
Viscous fingering in a modified Hele-Shaw cell is numerically investigated. The cell allows periodic variation of depth in the lateral direction. The wavenumber n of the depth perturbation has great influence on finge...Viscous fingering in a modified Hele-Shaw cell is numerically investigated. The cell allows periodic variation of depth in the lateral direction. The wavenumber n of the depth perturbation has great influence on fingering patterns. For n = 1, the fingering pattern due to the interface instability remains the same as that in the conventional Hele- Shaw cell, while the depth variation causes the steady finger to be a little narrower. For n = 2, four different fingering patterns are captured, similar to the available experimental observations in a modified Hele-Shaw cell containing a centered step-like occlusion. It is found that new fingering patterns appear as n further increases, among which, two patterns with spatial oscillation along both edges of the finger are particularly interesting. One is a symmetric oscillatory finger for n = 3, and the other is an asymmetric one for n = 4. The influence of capillary number on fingering patterns is studied for n = 3 and 4. We find that spatial oscillation of the finger nearly ceases at moderate capillary numbers and occurs again as the capillary number increases further. Meanwhile, the wide finger shifts to the narrow one. It is accompanied by a sudden decrease in the finger width which otherwise decreases continuously as the capillary number increases. The wavenumber and the amplitude of depth perturbation have little effect on the finger width.展开更多
We investigate the nonlinear dynamics of amoving interface in aHele-Shaw cell subject to an in-plane applied electric field.We develop a spectrally accurate numerical method for solving a coupled integral equation sys...We investigate the nonlinear dynamics of amoving interface in aHele-Shaw cell subject to an in-plane applied electric field.We develop a spectrally accurate numerical method for solving a coupled integral equation system.Although the stiffness due to the high order spatial derivatives can be removed using a small scale decomposition technique,the long-time simulation is still expensive since the evolving velocity of the interface drops dramatically as the interface expands.We remove this physically imposed stiffness by employing a rescaling scheme,which accelerates the slow dynamics and reduces the computational cost.Our nonlinear results reveal that positive currents restrain finger ramification and promote the overall stabilization of patterns.On the other hand,negative currents make the interface more unstable and lead to the formation of thin tail structures connecting the fingers and a small inner region.When no fluid is injected,and a negative current is utilized,the interface tends to approach the origin and break up into several drops.We investigate the temporal evolution of the smallest distance between the interface and the origin and find that it obeys an algebraic law(t∗−t)b,where t∗is the estimated pinch-off time.展开更多
Objective:Triple-negative breast cancer(TNBC)is highly aggressive and lacks an effective targeted therapy.This study aimed to elucidate the functions and possible mechanisms of action of zinc finger miz-type containin...Objective:Triple-negative breast cancer(TNBC)is highly aggressive and lacks an effective targeted therapy.This study aimed to elucidate the functions and possible mechanisms of action of zinc finger miz-type containing 2(ZMIZ2)and minichromosome maintenance complex component 3(MCM3)in TNBC progression.Methods:The relationship between ZMIZ2 expression and clinical characteristics of TNBC was investigated.In vitro and in vivo experiments were performed to investigate the role of ZMIZ2 dysregulation in TNBC cell malignant behaviors.The regulatory relationship between ZMIZ2 and MCM3 was also explored.Transcriptome sequencing was performed to elucidate possible mechanisms underlying the ZMIZ2/MCM3 axis in TNBC.Results:High ZMIZ2 expression levels were associated with the malignant degree of TNBC.ZMIZ2 overexpression promoted TNBC cell proliferation,migration,and invasion;inhibited apoptosis;and induced G1 phase cell cycle arrest,whereas knockdown of ZMIZ2 had the opposite effect.ZMIZ2 directly targeted and positively regulated MCM3 expression.MCM3 knockdown reversed the effect of ZMIZ2 overexpression on TNBC tumor growth both in vitro and in vivo.High MCM3 expression levels were linked to the degree of malignancy and poor prognosis in TNBC.The differentially expressed genes associated with the ZMIZ2/MCM3 axis were significantly enriched in multiple pathways,such as the mitogen-activated protein kinase(MAPK),mechanistic target of rapamycin(mTOR),Wnt,and Ras signaling pathways,as verified by The Cancer Genome Atlas data.Conclusions:ZMIZ2 and MCM3 were highly expressed in TNBC.ZMIZ2 promoted the development by positively regulating MCM3 expression.Key pathways,such as the Ras/MAPK,phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/mTOR,and Wnt signaling pathways,may be key downstreammechanisms.展开更多
Understanding and controlling fracture propagation is one of the most challenging engineering problems,especially in the oil and gas sector,groundwater hydrology,and geothermal energy applications.Predicting fracture ...Understanding and controlling fracture propagation is one of the most challenging engineering problems,especially in the oil and gas sector,groundwater hydrology,and geothermal energy applications.Predicting fracture orientation while possessing a nonlinear material response becomes more complex when the medium is subjected to anisotropic boundary stresses.Furthermore,the fracturing behaviour of geological porous media that exhibit high leak-off potential is not clearly understood.In this context,a novel testing technique is used to simulate in situ conditions in the laboratory and to study the instability characteristics of such geomaterials.The bespoke apparatus designed and developed in this research programme is capable of applying true anisotropic boundary stresses,injecting fluid at a predefined flow rate and viscosity while imaging instability/fracture propagation in a porous medium such as sand and bio-cemented sands.Pressure profiles and fracture propagation are simultaneously recorded during fluid injection into specimens subjected to different boundary stresses.Analysis of the images obtained during the fluid injection process provides information on the evolution of infiltration and fracture area during the injection event.The quantification of pressure and the corresponding fracture signature is,therefore,useful for a comprehensive understanding of the mechanics of fluid-induced fracture propagation.展开更多
BACKGROUND Hepatocellular carcinoma(HCC)is one of the most prevalent and aggressive forms of liver cancer,with high morbidity and poor prognosis due to late diagnosis and limited treatment options.Despite advances in ...BACKGROUND Hepatocellular carcinoma(HCC)is one of the most prevalent and aggressive forms of liver cancer,with high morbidity and poor prognosis due to late diagnosis and limited treatment options.Despite advances in understanding its molecular mechanisms,effective biomarkers for early detection and targeted therapy remain scarce.Zinc finger protein 71(ZNF71),a zinc-finger protein,has been implicated in various cancers,yet its role in HCC remains largely unexplored.This gap in knowledge underscores the need for further investigation into the ZNF71 of potential as a diagnostic or therapeutic target in HCC.AIM To explore the expression levels,clinical relevance,and molecular mechanisms of ZNF71 in the progression of HCC.METHODS The study evaluated ZNF71 expression in 235 HCC specimens and 13 noncancerous liver tissue samples using immunohistochemistry.High-throughput datasets were employed to assess the differential expression of ZNF71 in HCC and its association with clinical and pathological features.The impact of ZNF71 on HCC cell line growth was examined through clustered regularly interspaced short palindromic repeat knockout screens.Co-expressed genes were identified and analyzed for enrichment using LinkedOmics and Sangerbox 3.0,focusing on significant correlations(P<0.01,correlation coefficient≥0.3).Furthermore,the relationship between ZNF71 expression and immune cell infiltration was quantified using TIMER2.0.RESULTS ZNF71 showed higher expression in HCC tissues vs non-tumorous tissues,with a significant statistical difference(P<0.05).Data from the UALCAN platform indicated increased ZNF71 levels across early to mid-stage HCC,correlating with disease severity(P<0.05).High-throughput analysis presented a standardized mean difference in ZNF71 expression of 0.55(95%confidence interval[CI]:0.34-0.75).The efficiency of ZNF71 mRNA was evaluated,yielding an area under the curve of 0.78(95%CI:0.75-0.82),a sensitivity of 0.63(95%CI:0.53-0.72),and a specificity of 0.82(95%CI:0.73-0.89).Diagnostic likelihood ratios were positive at 3.61(95%CI:2.41-5.41)and negative at 0.45(95%CI:0.36-0.56).LinkedOmics analysis identified strong positive correlations of ZNF71 with genes such as ZNF470,ZNF256,and ZNF285.Pathway enrichment analyses highlighted associations with herpes simplex virus type 1 infection,the cell cycle,and DNA replication.Negative correlations involved metabolic pathways,peroxisomes,and fatty acid degradation.TIMER2.0 analysis demonstrated positive correlations of high ZNF71 expression with various immune cell types,including CD4^(+)T cells,B cells,regulatory T cells,monocytes,macrophages,and myeloid dendritic cells.CONCLUSION ZNF71 is significantly upregulated in HCC,correlating with the disease’s clinical and pathological stages.It appears to promote HCC progression through mechanisms involving the cell cycle and metabolism and is associated with immune cell infiltration.These findings suggest that ZNF71 could be a novel target for diagnosing and treating HCC.展开更多
PrPSc,a misfolded,aggregation-prone isoform of the cellular prion protein(PrPC),is the infectious prion agent responsible for fatal neurodegenerative diseases of humans and other mammals.PrPSccan adopt different patho...PrPSc,a misfolded,aggregation-prone isoform of the cellular prion protein(PrPC),is the infectious prion agent responsible for fatal neurodegenerative diseases of humans and other mammals.PrPSccan adopt different pathogenic conformations(prion strains),which can be resistant to potential drugs,or acquire drug resistance,posing challenges for the development of effective therapies.Since PrPCis the obligate precursor of any prion strain and serves as the mediator of prion neurotoxicity,it represents an attractive therapeutic target fo r prion diseases.In this minireview,we briefly outline the approaches to target PrPCand discuss our recent identification of Zn(Ⅱ)-Bn PyP,a PrPC-targeting porphyrin with an unprecedented bimodal mechanism of action.We argue that in-depth understanding of the molecular mechanism by which Zn(Ⅱ)-Bn PyP targets PrPCmay lead toward the development of a new class of dual mechanism anti-prion compounds.展开更多
BACKGROUND Colorectal cancer(CRC)is one of the most common causes of cancer mortality worldwide.The transcription factor Myc-associated zinc finger protein(MAZ)has been implicated in cancer progression.However,its pre...BACKGROUND Colorectal cancer(CRC)is one of the most common causes of cancer mortality worldwide.The transcription factor Myc-associated zinc finger protein(MAZ)has been implicated in cancer progression.However,its precise function and mecha-nisms in CRC remain unclear.AIM To investigate the role and mechanism of the MAZ/ubiquitin-like with PHD and RING finger domains 1(UHRF1)/esophageal cancer-related gene 4(ECRG4)axis in CRC metastasis.METHODS Western blot,quantitative reverse transcription polymerase chain reaction(PCR)and transwell were performed to evaluate the impact of MAZ knockdown on CRC cell migration and invasion.A xenograft tumor metastasis model was es-tablished by injecting MAZ-deficient CRC cells into nude mice to assess in vivo metastatic potential.Dual-luciferase reporter assay was performed to determine the role of MAZ and its downstream target,UHRF1.Chromatin immunoprecip-itation-quantitative PCR and methylation-specific PCR were used to analyze whether UHRF1 regulated ECRG4 through DNA methylation.RESULTS MAZ was highly upregulated in CRC cells and promoted CRC migration,inva-sion,epithelial-mesenchymal transition(EMT)and metastasis.Mechanistically,MAZ transcriptionally activated UHRF1,which in turn led to DNA methylation of ECRG4.Knockdown of MAZ suppressed CRC migration and invasion was reversed by overexpression of UHRF1.Loss of UHRF1 upregulated ECRG4,inhibited EMT,and reduced cell migration and invasion.However,simultaneous knockdown of ECRG4 partially reversed these effects.CONCLUSION MAZ promotes CRC cell migration,invasion,and EMT by transcriptionally activating UHRF1,which downreg-ulates ECRG4 through DNA methylation.展开更多
Wheat(Triticum aestivum L.)is a highly valued cereal crop produced and consumed globally,particularly in arid or semi-arid regions(Zhou et al.,2020;Mao et al.,2023).However,its production is increasingly threatened by...Wheat(Triticum aestivum L.)is a highly valued cereal crop produced and consumed globally,particularly in arid or semi-arid regions(Zhou et al.,2020;Mao et al.,2023).However,its production is increasingly threatened by the rising incidence of drought events associated with climate change.Arid regions are especially susceptible to these droughts,which are intensifying in both severity and frequency(Eckardt et al.,2023;Mao et al.,2023;Yang and Qin,2023).As of a 2022 report,more than 92%of wheat-producing regions are estimated to experience one or more drought/heatwave events in each growing season.Furthermore,the duration and frequency of these combined stress events have increased by approximately 28%over the past four decades(He et al.,2022).To address this challenge,wheat breeding programs have allocated substantial and research efforts to developing elite,stress tolerant lines.This initiative is large part by rapid innovation in transgenic and genome editing strategies(Hu and Xiong,2014;Gao et al.,2021.展开更多
Finger reimplantation is a pivotal technique in microsurgery,playing an irreplaceable role in restoring the functional integrity of fingers,maintaining the aesthetic appearance of the hand,ensuring the patient’s abil...Finger reimplantation is a pivotal technique in microsurgery,playing an irreplaceable role in restoring the functional integrity of fingers,maintaining the aesthetic appearance of the hand,ensuring the patient’s ability to perform delicate hand operations,and enhancing their social participation and quality of life.This paper reports on the perioperative nursing approach employed in a successful case of replantation involving a single hand with a multi-planar,20-segment amputation of four fingers.By establishing a specialized nursing team,conducting a comprehensive assessment of the patient’s condition,formulating a personalized nursing plan,and implementing precise nursing care,we achieved successful outcomes.After nearly four weeks of meticulous diagnosis,treatment,and nursing,17 segments of the replanted fingers survived,preserving the functional length of the amputated fingers and maximizing the restoration of hand function.This nursing method provides valuable insights and approaches for effectively managing vascular crises following digital replantation,improving the replantation survival rate,and enhancing patient satisfaction.展开更多
[Objectives]To explore the effects of motivational interviewing intervention based on the transtheoretical model(TTM)on psychological resilience and self-management in patients undergoing finger reimplantation after a...[Objectives]To explore the effects of motivational interviewing intervention based on the transtheoretical model(TTM)on psychological resilience and self-management in patients undergoing finger reimplantation after amputation.[Methods]The patients with finger replantation due to fractures admitted from October 2024 to June 2025 were divided into either the control group or the observation group according to the random number table method,with 40 cases in each group.The control group received conventional perioperative care,while the observation group underwent motivational interviewing based on TTM framework on the basis of the control group.The psychological resilience and self-management levels of the two patient groups were then compared following their respective care interventions.[Results]The psychological resilience and self-management scores of the patients were significantly higher than those of the control group after the intervention,with the difference being statistically significant(P<0.05).[Conclusions]Motivational interviewing based on the TTM can effectively improve the psychological resilience and self-management in patients undergoing severed finger reimplantation,while effectively reducing the occurrence of vascular crisis.展开更多
Background With the increasing prominence of hand and finger motion tracking in virtual reality(VR)applications and rehabilitation studies,data gloves have emerged as a prevalent solution.In this study,we developed an...Background With the increasing prominence of hand and finger motion tracking in virtual reality(VR)applications and rehabilitation studies,data gloves have emerged as a prevalent solution.In this study,we developed an innovative,lightweight,and detachable data glove tailored for finger motion tracking in VR environments.Methods The glove design incorporates a potentiometer coupled with a flexible rack and pinion gear system,facilitating precise and natural hand gestures for interaction with VR applications.Initially,we calibrated the potentiometer to align with the actual finger bending angle,and verified the accuracy of angle measurements recorded by the data glove.To verify the precision and reliability of our data glove,we conducted repeatability testing for flexion(grip test)and extension(flat test),with 250 measurements each,across five users.We employed the Gage Repeatability and Reproducibility to analyze and interpret the repeatable data.Furthermore,we integrated the gloves into a SteamVR home environment using the OpenGlove auto-calibration tool.Conclusions The repeatability analysis revealed an aggregate error of 1.45 degrees in both the gripped and flat hand positions.This outcome was notably favorable when compared with the findings from assessments of nine alternative data gloves that employed similar protocols.In these experiments,users navigated and engaged with virtual objects,underlining the glove's exact tracking of finger motion.Furthermore,the proposed data glove exhibited a low response time of 17-34 ms and back-drive force of only 0.19 N.Additionally,according to a comfort evaluation using the Comfort Rating Scales,the proposed glove system is wearable,placing it at the WL1 level.展开更多
BACKGROUND Colorectal cancer(CRC)is one of the most common malignant gastrointestinal tumors worldwide,with high incidence and mortality rates.AIM To investigate the expression significance of the chromatin-remodeling...BACKGROUND Colorectal cancer(CRC)is one of the most common malignant gastrointestinal tumors worldwide,with high incidence and mortality rates.AIM To investigate the expression significance of the chromatin-remodeling protein MORC family CW-type zinc finger 4(MORC4)as a biomarker in CRC patients,and to explore its relationship with pathological features and prognosis.METHODS A total of 143 CRC specimens and 57 adjacent tissue specimens,surgically removed from our hospital between January 2020 and January 2021,were collected.MORC4 protein expression was assessed using immunohistochemistry after paraffin embedding.The relationship between MORC4 protein expression and clinicopathological characteristics of patients was analyzed.Kaplan-Meier survival curves were plotted to analyze the relationship between MORC4 protein expression and prognosis in CRC patients.RESULTS Compared with adjacent tissues,the expression rate of MORC4 protein in CRC tissues was significantly higher(P<0.05).No significant difference was observed in the high expression rate of MORC4 protein in CRC tissues among patients of different gender,age,tumor location,tumor diameter,and primary tumor status(P>0.05).However,significant differences were found in the high expression rate of MORC4 protein in patients with different degrees of differentiation,lymph node metastasis,distant metastasis,tumor-lymph node-metastasis stage,and serum carcinoembryonic antigen levels(P<0.05).Compared with patients with low MORC4 expression,patients with high MORC4 expression had a worse prognosis(P<0.05).CONCLUSION The upregulation of MORC4 expression in CRC patients is closely related to disease severity and prognosis,suggesting its potential as an evaluation biomarker,which warrants further investigation.展开更多
Following the publication of Xu et al.(2022),an error was identified in Figure 1D.Specifically,the top left panel was inadvertently duplicated during figure preparation.To ensure the accuracy and integrity of our publ...Following the publication of Xu et al.(2022),an error was identified in Figure 1D.Specifically,the top left panel was inadvertently duplicated during figure preparation.To ensure the accuracy and integrity of our published work,we request the publication of a corrigendum with the corrected image.We apologize for this oversight and any confusion it may have caused.The amended figure is provided in the updated Supplementary Materials.展开更多
Objectives:Recently,we and others have demonstrated the involvement of Zinc Finger Antisense 1(ZFAS1)in cancer development.However,the intricate interplay of ZFAS1 with miRNAs and mRNAs remains to be fully understood....Objectives:Recently,we and others have demonstrated the involvement of Zinc Finger Antisense 1(ZFAS1)in cancer development.However,the intricate interplay of ZFAS1 with miRNAs and mRNAs remains to be fully understood.Materials and methods:We followed PRISMA guidelines to retrieve and assess the available literature on the topic“ZFAS1/miRNA/mRNA axis”and“Cancer”from databases such as PubMed,Google Scholar,and ScienceDirect.We also used bioinformatic webtools for analyzing the potential miRNA targets of ZFAS1 and its role in survival of cancer patients along with their role in various biological functions and pathways.Results:Our literature search and bioinformatic analysis reveals that ZFAS1 serves as a sponge for numerous miRNAs.Among the various targeted miRNAs,miR-150-5p stands out as significantly correlated with ZFAS1 across multiple databases(p-value=3.27e-16,R-value=-0.346).Additionally,our Kaplan-Meier survival analysis indicates a noteworthy association between ZFAS1 expression levels and overall poor prognosis and survival rates in ovarian,sarcoma,and pancreatic cancers.We also underscore the involvement of various signaling pathways,including Signal Transducer and Activator of Transcription 3(STAT3),Spindle and Kinetochore-associated Protein 1(SKA1),Lysophosphatidic acid receptor 1(LPAR1),and Wntβ-catenin,in cancer development through the ZFAS1/miRNAs/mRNAs axis.Furthermore,we identify ZFAS1’s pivotal roles in diverse molecular processes,such as RNA binding and ribonucleoprotein formation.Conclusion:In conclusion,this review comprehensively summarizes the latest advancements in understanding the regulatory relationships among ZFAS1,miRNAs,and mRNAs,emphasizing their collective role in cancer development to propose innovative avenues for cancer treatment.We believe that the intricate relationship among the ZFAS1-miRNA-mRNA axis may yield potential therapeutic targets for effective cancer management.展开更多
Genome editing in plants is a powerful strategy that can substantially advance functional genomics research,facilitating the discovery,enhancement,and development of novel traits with significant agricultural implicat...Genome editing in plants is a powerful strategy that can substantially advance functional genomics research,facilitating the discovery,enhancement,and development of novel traits with significant agricultural implications.Various methodologies,such as zinc finger nucleases(ZFNs),transcription activator-like effector nucleases(TALENs),and CRISPR/Cas systems,have been developed for this purpose.展开更多
基金supported by the Research and Innovation Fund for Graduate Students of Southwest Petroleum University(No.2022KYCX027)supported by the National Natural Science Foundation for Youth Grant(No.41902157).
文摘Studying immiscible fluid displacement patterns can provide a better understanding of displacement processes within heterogeneous porous media,thereby helping improving oil recovery and optimizing geological CO_(2) sequestration.As the injection rate of water displacing oil increases and the displacement pattern transits from capillary fingering to viscous fingering,there is a broad crossover zone between the two that can adversely affect the oil displacement efficiency.While previous studies have utilized phase diagrams to investigate the influence of the viscosity ratio and wettability of the crossover zone,fewer have studied the impact of rock heterogeneity.In this study,we created pore network models with varying degrees of heterogeneity to simulate water flooding at different injection rates.Our model quantifies capillary and viscous fingering characteristics while investigating porous media heterogeneity's role in the crossover zone.Analysis of simulation results reveals that a higher characteristic front flow rate within the crossover zone leads to earlier breakthrough and reduced displacement efficiency.Increased heterogeneity in the porous media raises injection-site pressure,lowers water saturation,and elevates the characteristic front flow rate,thereby expanding the extent of crossover zone.
基金Shiraz University Enhanced Oil Recovery(EOR)Research Center for the support
文摘Viscous fingering is one of the main challenges that could reduce areal sweep efficiency during waterflooding in oil reservoirs. A series of waterflooding experiments were carried out in a Hele-Shaw cell at ambient temperature during which areal sweep efficiency was estimated and techniques to ease the fingering problem were examined. The onset and propagation of viscous fingers were monitored as a function of both injection rate and injection/production positions. Image processing techniques were utilized to quantitatively investigate the propagation of fingers. The experimental results show that, under specific conditions, increasing the number of finger branches could improve the areal sweep efficiency, whereas growth of a single narrow finger has a negative impact on oil displacement efficiency. According to the obtained results,increasing the injection rate improves the areal sweep efficiency up to a critical rate at which viscous fingers start to grow.The impact of heterogeneity of the medium on distributing the viscous fingers was also investigated by introducing two different arrangements of fractures in the model. The results show that fractures perpendicular to the direction of flow would distribute the displacing water more uniformly, while fractures in the direction of flow would amplify the unfavorable sweep efficiency.
基金supported by the NSFC sponsored project under grant(50976017)(50736001)
文摘Fingering phenomena are common occurrence in the natural world. It generally takes place when a less viscous fluid displacesa more viscous fluid typically in porous media. Nowadays, such phenomena have extensively been studied due to itsimportance in many industrial fields. In this paper, the effects of surface wettability on finger pattern are studied and simulatednumerically by the Lattice Boltzmann method (LBM). The displacement efficiency is investigated by using two parameters,namely, the breakthrough time and the areal sweep efficiency. The simulation has demonstrated that surface wettability willinfluence the finger pattern no matter the gravity is considered or not, but in the presence of gravity, the finger pattern is muchmore complicated and irregular due to the coexistence and competition of capillary force, viscous force and gravity.
基金supported by the National Natural Science Foundation of China Joint Fund Project (Grant/Award Number: U20B6003)National Natural Science Foundation of China (Grant/Award Number: 52304054)。
文摘Understanding fingering, as a challenge to stable displacement during the immiscible flow, has become a crucial phenomenon for geological carbon sequestration, enhanced oil recovery, and groundwater protection. Typically governed by gravity, viscous and capillary forces, these factors lead invasive fluids to occupy pore space irregularly and incompletely. Previous studies have demonstrated capillary numbers,describing the viscous and capillary forces, to quantificationally induce evolution of invasion patterns.While the evolution mechanisms of invasive patterns have not been deeply elucidated under the constant capillary number and three variable parameters including velocity, viscosity, and interfacial tension.Our research employs two horizontal visualization systems and a two-phase laminar flow simulation to investigate the tendency of invasive pattern transition by various parameters at the pore scale. We showed that increasing invasive viscosity or reducing interfacial tension in a homogeneous pore space significantly enhanced sweep efficiency, under constant capillary number. Additionally, in the fingering crossover pattern, the region near the inlet was prone to capillary fingering with multi-directional invasion, while the viscous fingering with unidirectional invasion was more susceptible occurred in the region near the outlet. Furthermore, increasing invasive viscosity or decreasing invasive velocity and interfacial tension promoted the extension of viscous fingering from the outlet to the inlet, presenting that the subsequent invasive fluid flows toward the outlet. In the case of invasive trunk along a unidirectional path, the invasive flow increased exponentially closer to the outlet, resulting in a significant decrease in the width of the invasive interface. Our work holds promising applications for optimizing invasive patterns in heterogeneous porous media.
基金Project supported by the National Natural Science Foundation of China(No.11232011)the 111 Project of China(No.B07033)
文摘Viscous fingering in a modified Hele-Shaw cell is numerically investigated. The cell allows periodic variation of depth in the lateral direction. The wavenumber n of the depth perturbation has great influence on fingering patterns. For n = 1, the fingering pattern due to the interface instability remains the same as that in the conventional Hele- Shaw cell, while the depth variation causes the steady finger to be a little narrower. For n = 2, four different fingering patterns are captured, similar to the available experimental observations in a modified Hele-Shaw cell containing a centered step-like occlusion. It is found that new fingering patterns appear as n further increases, among which, two patterns with spatial oscillation along both edges of the finger are particularly interesting. One is a symmetric oscillatory finger for n = 3, and the other is an asymmetric one for n = 4. The influence of capillary number on fingering patterns is studied for n = 3 and 4. We find that spatial oscillation of the finger nearly ceases at moderate capillary numbers and occurs again as the capillary number increases further. Meanwhile, the wide finger shifts to the narrow one. It is accompanied by a sudden decrease in the finger width which otherwise decreases continuously as the capillary number increases. The wavenumber and the amplitude of depth perturbation have little effect on the finger width.
基金the National Science Foundation,Division of Mathematical Sciences(NSF-DMS)grants DMS-1714973,1719960,1763272(J.L.)DMS-1720420(S.L.).J.L.thanks the support from the Simons Foundation(594598QN)for a NSF-Simons Center for Multiscale Cell Fate Research.J.L.also thanks the National Institutes of Health for partial support through grants 1U54CA217378-01A1 for a National Center in Cancer Systems Biology at UC Irvine and P30CA062203 for the Chao Family Comprehensive Cancer Center at UC Irvine.
文摘We investigate the nonlinear dynamics of amoving interface in aHele-Shaw cell subject to an in-plane applied electric field.We develop a spectrally accurate numerical method for solving a coupled integral equation system.Although the stiffness due to the high order spatial derivatives can be removed using a small scale decomposition technique,the long-time simulation is still expensive since the evolving velocity of the interface drops dramatically as the interface expands.We remove this physically imposed stiffness by employing a rescaling scheme,which accelerates the slow dynamics and reduces the computational cost.Our nonlinear results reveal that positive currents restrain finger ramification and promote the overall stabilization of patterns.On the other hand,negative currents make the interface more unstable and lead to the formation of thin tail structures connecting the fingers and a small inner region.When no fluid is injected,and a negative current is utilized,the interface tends to approach the origin and break up into several drops.We investigate the temporal evolution of the smallest distance between the interface and the origin and find that it obeys an algebraic law(t∗−t)b,where t∗is the estimated pinch-off time.
基金supported by the Jilin Province Health Science and Technology Ability Improvement Project(2023JL057).
文摘Objective:Triple-negative breast cancer(TNBC)is highly aggressive and lacks an effective targeted therapy.This study aimed to elucidate the functions and possible mechanisms of action of zinc finger miz-type containing 2(ZMIZ2)and minichromosome maintenance complex component 3(MCM3)in TNBC progression.Methods:The relationship between ZMIZ2 expression and clinical characteristics of TNBC was investigated.In vitro and in vivo experiments were performed to investigate the role of ZMIZ2 dysregulation in TNBC cell malignant behaviors.The regulatory relationship between ZMIZ2 and MCM3 was also explored.Transcriptome sequencing was performed to elucidate possible mechanisms underlying the ZMIZ2/MCM3 axis in TNBC.Results:High ZMIZ2 expression levels were associated with the malignant degree of TNBC.ZMIZ2 overexpression promoted TNBC cell proliferation,migration,and invasion;inhibited apoptosis;and induced G1 phase cell cycle arrest,whereas knockdown of ZMIZ2 had the opposite effect.ZMIZ2 directly targeted and positively regulated MCM3 expression.MCM3 knockdown reversed the effect of ZMIZ2 overexpression on TNBC tumor growth both in vitro and in vivo.High MCM3 expression levels were linked to the degree of malignancy and poor prognosis in TNBC.The differentially expressed genes associated with the ZMIZ2/MCM3 axis were significantly enriched in multiple pathways,such as the mitogen-activated protein kinase(MAPK),mechanistic target of rapamycin(mTOR),Wnt,and Ras signaling pathways,as verified by The Cancer Genome Atlas data.Conclusions:ZMIZ2 and MCM3 were highly expressed in TNBC.ZMIZ2 promoted the development by positively regulating MCM3 expression.Key pathways,such as the Ras/MAPK,phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/mTOR,and Wnt signaling pathways,may be key downstreammechanisms.
基金supported and funded by the British Petroleum-International Centre for Advanced Materials(BP-ICAM)on project ICAM-39(Water Injection in Soft Sand Reservoirs)the University of Cambridge,UK.Their support is gratefully acknowledged.
文摘Understanding and controlling fracture propagation is one of the most challenging engineering problems,especially in the oil and gas sector,groundwater hydrology,and geothermal energy applications.Predicting fracture orientation while possessing a nonlinear material response becomes more complex when the medium is subjected to anisotropic boundary stresses.Furthermore,the fracturing behaviour of geological porous media that exhibit high leak-off potential is not clearly understood.In this context,a novel testing technique is used to simulate in situ conditions in the laboratory and to study the instability characteristics of such geomaterials.The bespoke apparatus designed and developed in this research programme is capable of applying true anisotropic boundary stresses,injecting fluid at a predefined flow rate and viscosity while imaging instability/fracture propagation in a porous medium such as sand and bio-cemented sands.Pressure profiles and fracture propagation are simultaneously recorded during fluid injection into specimens subjected to different boundary stresses.Analysis of the images obtained during the fluid injection process provides information on the evolution of infiltration and fracture area during the injection event.The quantification of pressure and the corresponding fracture signature is,therefore,useful for a comprehensive understanding of the mechanics of fluid-induced fracture propagation.
基金Supported by Joint Project on Regional High Incidence Diseases Research of Guangxi Natural Science Foundation,No.2024GXNSFAA010057 and No.2024GXNSFAA010085Natural Science Foundation of Guangxi,China,No.2022GXNSFBA035657+2 种基金Guangxi Zhuang Autonomous Region Health Commission Self-Financed Scientific Research Project,No.Z20210764Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine Scientific Research Project,No.GXZYA20230270 and No.GXZYA20240305Advanced Innovation Teams and Xinghu Scholars Program of Guangxi Medical University(2022).
文摘BACKGROUND Hepatocellular carcinoma(HCC)is one of the most prevalent and aggressive forms of liver cancer,with high morbidity and poor prognosis due to late diagnosis and limited treatment options.Despite advances in understanding its molecular mechanisms,effective biomarkers for early detection and targeted therapy remain scarce.Zinc finger protein 71(ZNF71),a zinc-finger protein,has been implicated in various cancers,yet its role in HCC remains largely unexplored.This gap in knowledge underscores the need for further investigation into the ZNF71 of potential as a diagnostic or therapeutic target in HCC.AIM To explore the expression levels,clinical relevance,and molecular mechanisms of ZNF71 in the progression of HCC.METHODS The study evaluated ZNF71 expression in 235 HCC specimens and 13 noncancerous liver tissue samples using immunohistochemistry.High-throughput datasets were employed to assess the differential expression of ZNF71 in HCC and its association with clinical and pathological features.The impact of ZNF71 on HCC cell line growth was examined through clustered regularly interspaced short palindromic repeat knockout screens.Co-expressed genes were identified and analyzed for enrichment using LinkedOmics and Sangerbox 3.0,focusing on significant correlations(P<0.01,correlation coefficient≥0.3).Furthermore,the relationship between ZNF71 expression and immune cell infiltration was quantified using TIMER2.0.RESULTS ZNF71 showed higher expression in HCC tissues vs non-tumorous tissues,with a significant statistical difference(P<0.05).Data from the UALCAN platform indicated increased ZNF71 levels across early to mid-stage HCC,correlating with disease severity(P<0.05).High-throughput analysis presented a standardized mean difference in ZNF71 expression of 0.55(95%confidence interval[CI]:0.34-0.75).The efficiency of ZNF71 mRNA was evaluated,yielding an area under the curve of 0.78(95%CI:0.75-0.82),a sensitivity of 0.63(95%CI:0.53-0.72),and a specificity of 0.82(95%CI:0.73-0.89).Diagnostic likelihood ratios were positive at 3.61(95%CI:2.41-5.41)and negative at 0.45(95%CI:0.36-0.56).LinkedOmics analysis identified strong positive correlations of ZNF71 with genes such as ZNF470,ZNF256,and ZNF285.Pathway enrichment analyses highlighted associations with herpes simplex virus type 1 infection,the cell cycle,and DNA replication.Negative correlations involved metabolic pathways,peroxisomes,and fatty acid degradation.TIMER2.0 analysis demonstrated positive correlations of high ZNF71 expression with various immune cell types,including CD4^(+)T cells,B cells,regulatory T cells,monocytes,macrophages,and myeloid dendritic cells.CONCLUSION ZNF71 is significantly upregulated in HCC,correlating with the disease’s clinical and pathological stages.It appears to promote HCC progression through mechanisms involving the cell cycle and metabolism and is associated with immune cell infiltration.These findings suggest that ZNF71 could be a novel target for diagnosing and treating HCC.
基金supported by Telethon Italy award GGP15225(to RC and GM)Italian Ministry of Health award RF-2016-02362950(to RC and CZ)+1 种基金the CJD Foundation USA(to RC)the Associazione Italiana Encefalopatie da Prioni(AIEnP)(to RC).
文摘PrPSc,a misfolded,aggregation-prone isoform of the cellular prion protein(PrPC),is the infectious prion agent responsible for fatal neurodegenerative diseases of humans and other mammals.PrPSccan adopt different pathogenic conformations(prion strains),which can be resistant to potential drugs,or acquire drug resistance,posing challenges for the development of effective therapies.Since PrPCis the obligate precursor of any prion strain and serves as the mediator of prion neurotoxicity,it represents an attractive therapeutic target fo r prion diseases.In this minireview,we briefly outline the approaches to target PrPCand discuss our recent identification of Zn(Ⅱ)-Bn PyP,a PrPC-targeting porphyrin with an unprecedented bimodal mechanism of action.We argue that in-depth understanding of the molecular mechanism by which Zn(Ⅱ)-Bn PyP targets PrPCmay lead toward the development of a new class of dual mechanism anti-prion compounds.
基金Supported by Hangzhou Medical and Health Science and Technology Plan,No.B20210014.
文摘BACKGROUND Colorectal cancer(CRC)is one of the most common causes of cancer mortality worldwide.The transcription factor Myc-associated zinc finger protein(MAZ)has been implicated in cancer progression.However,its precise function and mecha-nisms in CRC remain unclear.AIM To investigate the role and mechanism of the MAZ/ubiquitin-like with PHD and RING finger domains 1(UHRF1)/esophageal cancer-related gene 4(ECRG4)axis in CRC metastasis.METHODS Western blot,quantitative reverse transcription polymerase chain reaction(PCR)and transwell were performed to evaluate the impact of MAZ knockdown on CRC cell migration and invasion.A xenograft tumor metastasis model was es-tablished by injecting MAZ-deficient CRC cells into nude mice to assess in vivo metastatic potential.Dual-luciferase reporter assay was performed to determine the role of MAZ and its downstream target,UHRF1.Chromatin immunoprecip-itation-quantitative PCR and methylation-specific PCR were used to analyze whether UHRF1 regulated ECRG4 through DNA methylation.RESULTS MAZ was highly upregulated in CRC cells and promoted CRC migration,inva-sion,epithelial-mesenchymal transition(EMT)and metastasis.Mechanistically,MAZ transcriptionally activated UHRF1,which in turn led to DNA methylation of ECRG4.Knockdown of MAZ suppressed CRC migration and invasion was reversed by overexpression of UHRF1.Loss of UHRF1 upregulated ECRG4,inhibited EMT,and reduced cell migration and invasion.However,simultaneous knockdown of ECRG4 partially reversed these effects.CONCLUSION MAZ promotes CRC cell migration,invasion,and EMT by transcriptionally activating UHRF1,which downreg-ulates ECRG4 through DNA methylation.
基金supported by grants from the Major Project on Agricultural Bio-breeding of China(2023ZD04026)National Natural Science Foundation of China(31872866 and 32372124)+2 种基金China Postdoctoral Science Foundation(2022M721101)National Natural Science Foundation of Hunan(2023JJ40132)Natural Science Foundation of Chongqing(CSTB2023NSCQ-MSX0542).
文摘Wheat(Triticum aestivum L.)is a highly valued cereal crop produced and consumed globally,particularly in arid or semi-arid regions(Zhou et al.,2020;Mao et al.,2023).However,its production is increasingly threatened by the rising incidence of drought events associated with climate change.Arid regions are especially susceptible to these droughts,which are intensifying in both severity and frequency(Eckardt et al.,2023;Mao et al.,2023;Yang and Qin,2023).As of a 2022 report,more than 92%of wheat-producing regions are estimated to experience one or more drought/heatwave events in each growing season.Furthermore,the duration and frequency of these combined stress events have increased by approximately 28%over the past four decades(He et al.,2022).To address this challenge,wheat breeding programs have allocated substantial and research efforts to developing elite,stress tolerant lines.This initiative is large part by rapid innovation in transgenic and genome editing strategies(Hu and Xiong,2014;Gao et al.,2021.
文摘Finger reimplantation is a pivotal technique in microsurgery,playing an irreplaceable role in restoring the functional integrity of fingers,maintaining the aesthetic appearance of the hand,ensuring the patient’s ability to perform delicate hand operations,and enhancing their social participation and quality of life.This paper reports on the perioperative nursing approach employed in a successful case of replantation involving a single hand with a multi-planar,20-segment amputation of four fingers.By establishing a specialized nursing team,conducting a comprehensive assessment of the patient’s condition,formulating a personalized nursing plan,and implementing precise nursing care,we achieved successful outcomes.After nearly four weeks of meticulous diagnosis,treatment,and nursing,17 segments of the replanted fingers survived,preserving the functional length of the amputated fingers and maximizing the restoration of hand function.This nursing method provides valuable insights and approaches for effectively managing vascular crises following digital replantation,improving the replantation survival rate,and enhancing patient satisfaction.
基金Supported by 2023 Shiyan Taihe Hospital Fund Project(2023JJXM024).
文摘[Objectives]To explore the effects of motivational interviewing intervention based on the transtheoretical model(TTM)on psychological resilience and self-management in patients undergoing finger reimplantation after amputation.[Methods]The patients with finger replantation due to fractures admitted from October 2024 to June 2025 were divided into either the control group or the observation group according to the random number table method,with 40 cases in each group.The control group received conventional perioperative care,while the observation group underwent motivational interviewing based on TTM framework on the basis of the control group.The psychological resilience and self-management levels of the two patient groups were then compared following their respective care interventions.[Results]The psychological resilience and self-management scores of the patients were significantly higher than those of the control group after the intervention,with the difference being statistically significant(P<0.05).[Conclusions]Motivational interviewing based on the TTM can effectively improve the psychological resilience and self-management in patients undergoing severed finger reimplantation,while effectively reducing the occurrence of vascular crisis.
基金Supported by the Sirindhorn International Institute of Technology,Thammasat University,EFS-G(Excellent foreign Student-Graduate)research fund.
文摘Background With the increasing prominence of hand and finger motion tracking in virtual reality(VR)applications and rehabilitation studies,data gloves have emerged as a prevalent solution.In this study,we developed an innovative,lightweight,and detachable data glove tailored for finger motion tracking in VR environments.Methods The glove design incorporates a potentiometer coupled with a flexible rack and pinion gear system,facilitating precise and natural hand gestures for interaction with VR applications.Initially,we calibrated the potentiometer to align with the actual finger bending angle,and verified the accuracy of angle measurements recorded by the data glove.To verify the precision and reliability of our data glove,we conducted repeatability testing for flexion(grip test)and extension(flat test),with 250 measurements each,across five users.We employed the Gage Repeatability and Reproducibility to analyze and interpret the repeatable data.Furthermore,we integrated the gloves into a SteamVR home environment using the OpenGlove auto-calibration tool.Conclusions The repeatability analysis revealed an aggregate error of 1.45 degrees in both the gripped and flat hand positions.This outcome was notably favorable when compared with the findings from assessments of nine alternative data gloves that employed similar protocols.In these experiments,users navigated and engaged with virtual objects,underlining the glove's exact tracking of finger motion.Furthermore,the proposed data glove exhibited a low response time of 17-34 ms and back-drive force of only 0.19 N.Additionally,according to a comfort evaluation using the Comfort Rating Scales,the proposed glove system is wearable,placing it at the WL1 level.
基金was approved by the Ethics Committee of Cangzhou Central Hospital,No.29795793.
文摘BACKGROUND Colorectal cancer(CRC)is one of the most common malignant gastrointestinal tumors worldwide,with high incidence and mortality rates.AIM To investigate the expression significance of the chromatin-remodeling protein MORC family CW-type zinc finger 4(MORC4)as a biomarker in CRC patients,and to explore its relationship with pathological features and prognosis.METHODS A total of 143 CRC specimens and 57 adjacent tissue specimens,surgically removed from our hospital between January 2020 and January 2021,were collected.MORC4 protein expression was assessed using immunohistochemistry after paraffin embedding.The relationship between MORC4 protein expression and clinicopathological characteristics of patients was analyzed.Kaplan-Meier survival curves were plotted to analyze the relationship between MORC4 protein expression and prognosis in CRC patients.RESULTS Compared with adjacent tissues,the expression rate of MORC4 protein in CRC tissues was significantly higher(P<0.05).No significant difference was observed in the high expression rate of MORC4 protein in CRC tissues among patients of different gender,age,tumor location,tumor diameter,and primary tumor status(P>0.05).However,significant differences were found in the high expression rate of MORC4 protein in patients with different degrees of differentiation,lymph node metastasis,distant metastasis,tumor-lymph node-metastasis stage,and serum carcinoembryonic antigen levels(P<0.05).Compared with patients with low MORC4 expression,patients with high MORC4 expression had a worse prognosis(P<0.05).CONCLUSION The upregulation of MORC4 expression in CRC patients is closely related to disease severity and prognosis,suggesting its potential as an evaluation biomarker,which warrants further investigation.
文摘Following the publication of Xu et al.(2022),an error was identified in Figure 1D.Specifically,the top left panel was inadvertently duplicated during figure preparation.To ensure the accuracy and integrity of our published work,we request the publication of a corrigendum with the corrected image.We apologize for this oversight and any confusion it may have caused.The amended figure is provided in the updated Supplementary Materials.
文摘Objectives:Recently,we and others have demonstrated the involvement of Zinc Finger Antisense 1(ZFAS1)in cancer development.However,the intricate interplay of ZFAS1 with miRNAs and mRNAs remains to be fully understood.Materials and methods:We followed PRISMA guidelines to retrieve and assess the available literature on the topic“ZFAS1/miRNA/mRNA axis”and“Cancer”from databases such as PubMed,Google Scholar,and ScienceDirect.We also used bioinformatic webtools for analyzing the potential miRNA targets of ZFAS1 and its role in survival of cancer patients along with their role in various biological functions and pathways.Results:Our literature search and bioinformatic analysis reveals that ZFAS1 serves as a sponge for numerous miRNAs.Among the various targeted miRNAs,miR-150-5p stands out as significantly correlated with ZFAS1 across multiple databases(p-value=3.27e-16,R-value=-0.346).Additionally,our Kaplan-Meier survival analysis indicates a noteworthy association between ZFAS1 expression levels and overall poor prognosis and survival rates in ovarian,sarcoma,and pancreatic cancers.We also underscore the involvement of various signaling pathways,including Signal Transducer and Activator of Transcription 3(STAT3),Spindle and Kinetochore-associated Protein 1(SKA1),Lysophosphatidic acid receptor 1(LPAR1),and Wntβ-catenin,in cancer development through the ZFAS1/miRNAs/mRNAs axis.Furthermore,we identify ZFAS1’s pivotal roles in diverse molecular processes,such as RNA binding and ribonucleoprotein formation.Conclusion:In conclusion,this review comprehensively summarizes the latest advancements in understanding the regulatory relationships among ZFAS1,miRNAs,and mRNAs,emphasizing their collective role in cancer development to propose innovative avenues for cancer treatment.We believe that the intricate relationship among the ZFAS1-miRNA-mRNA axis may yield potential therapeutic targets for effective cancer management.
基金supported by the Agricultural Gongguan Project of Xinjiang Production and Construction Corps,China(NYHXGG,2023AA102)the Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-CSIAF-202402)+1 种基金the Outstanding Youth Foundation of Henan Scientific Committee,China(222300420097)the China Agriculture Research System of MOF and MARA(CARS-15-02).
文摘Genome editing in plants is a powerful strategy that can substantially advance functional genomics research,facilitating the discovery,enhancement,and development of novel traits with significant agricultural implications.Various methodologies,such as zinc finger nucleases(ZFNs),transcription activator-like effector nucleases(TALENs),and CRISPR/Cas systems,have been developed for this purpose.
