An effective flotation approach is proposed for improving the recovery of molybdenite fines from a finely-disseminated molybdenum ore. To maximize the flotation recovery of molybdenum, process mineralogy of raw ore, c...An effective flotation approach is proposed for improving the recovery of molybdenite fines from a finely-disseminated molybdenum ore. To maximize the flotation recovery of molybdenum, process mineralogy of raw ore, contrast tests, optimization of operation conditions and particle size analysis were systematically investigated. Process mineralogy suggests that in the raw ore, 61.63% of molybdenite particles distribute in the 〈20 pm size fraction, and intergrow with muscovite and pyrite as the contained and disseminated type. Contrast tests indicate that conventional flotation responds to poor collection efficiency for particles less than 25 pm. Oil agglomerate flotation （OAF） process demonstrates an obvious superiority in improving the flotation recovery of molybdenite fines. Furthermore, the flotation results of OAF process reveal that the dosage of transformer oil plays a critical role on the average size of collected mineral particles （d（0）, agglomerates （d^0） and the molybdenum recovery. In addition, industrial tests illustrate that compared with the Mo-S bulk flotation approach, OAF process not only increases Mo recovery and grade of molybdenum concentrate by 22.75% and 17.47% respectively, but also achieves a sulfur concentrate with a superior grade of 38.92%.展开更多
We investigated the effects of finely ground wheat bran on the nutrient digestibility, digesta passage rate,and gut microbiota structure in sows. A 3 × 3 Latin square design with 3 test periods and 3 experimental...We investigated the effects of finely ground wheat bran on the nutrient digestibility, digesta passage rate,and gut microbiota structure in sows. A 3 × 3 Latin square design with 3 test periods and 3 experimental diets was used. Six non-pregnant sows(parity: 5 to 7) were randomly assigned to 3 experimental diets with 2 replicates per treatment in each period. Each period lasted 19 d(12 d for adaptation and 7 d for experiment). The experimental diets included(a) a basal corn and soybean meal diet(CON),(b) a basal diet with 20% coarse wheat bran(CWB;particle size: 605 μm), and(c) a basal diet with 20% fine wheat bran(FWB;particle size: 438 μm). The results demonstrated that the apparent total tract digestibility of neutral detergent fiber, acid detergent fiber and energy were reduced(P < 0.05) in the FWB and CWB groups compared with those in the CON group. Viscosity of digesta increased(P < 0.001) in FWB-fed sows. The passage rate of digesta from the mouth to the ileum decreased(P < 0.001) in FWB-fed sows. Peptide YY(PYY) concentration increased(P = 0.01) in FWB-fed sows after 30 min of feeding.In the FWB group, the relative abundance of Lactobacillaceae at the family level increased(P < 0.05) in the ileal digesta. At the class level, the relative abundance of Clostridia in feces decreased(P < 0.05) in FWB-fed sows. FWB enhanced the concentration of butyrate in feces compared with CON and CWB(P = 0.04). These results suggest that dietary supplementation with finely ground wheat bran reduces the passage rate of digesta, increases the abundance of beneficial microorganisms, and elevates the concentration of short-chain fatty acids and PYY in sows. These findings indicate that the addition of finelyground wheat bran to the diets of sows is more effective than using coarse wheat bran for improving their satiety and intestinal microbial composition.展开更多
Particle coatings are used extensively to generate dispersed solids with well-defined properties, e.g., to protect active ingredients, with most coating processes using core particles of a diameter larger than 200 μm...Particle coatings are used extensively to generate dispersed solids with well-defined properties, e.g., to protect active ingredients, with most coating processes using core particles of a diameter larger than 200 μm. This work contributes to the development of a coating process for fine dispersed particles （diam- eter less than 50 μm） by combining two particle-formulation processes, namely, coating and spray drying. The feasibility of the operation is based on and demonstrated by the innovative application of a two-fluid nozzle. Experiments were conducted by using glass particles as core particles and sodium benzoate as the coating agent. The coating of finely dispersed particles is achieved by the spraying of particles and coating solution as a homogeneous suspension. The aim is to create droplets with only one contained particle at the nozzle outlet. After evaporation of the water in the droplet, a thin solid film is built on the particle surface. The suspension viscosity was measured and compared with empirical equations from the literature. The liquid-film thickness on the particle surface was calculated to predict the building of a uniform coating layer or agglomerates. In this study, the feasibility of pneumatic transport through the nozzle and an investigation of the process were illustrated. The agglomeration fraction and degree of coating of the particle surface were analyzed optically by scanning electron microscopy. In this way, the influence of different processes and suspension parameters on the product quality were determined.展开更多
Pancreatic cysts are mostly incidental findings on computed tomography or magnetic resonance imaging scans,with few patients presenting with abdominal pain or other symptoms.The accurate diagnosis of cysts is importan...Pancreatic cysts are mostly incidental findings on computed tomography or magnetic resonance imaging scans,with few patients presenting with abdominal pain or other symptoms.The accurate diagnosis of cysts is important as management depends on the type(neoplastic or non-neoplastic).Cross-sectional imaging is fast being replaced with endoscopic ultrasound(EUS)and various techniques based on that such as EUS-guided fine needle aspiration,EUS-guided needle confocal laser endomicroscopy,EUS-through-the-needle biopsy,and contrast-enhanced EUS.Clinical studies have reported varying diagnostic and adverse event rates with these modalities.In addition,American,European,and Kyoto guidelines for the diagnosis and management of pancreatic cysts have provided different recommendations.In this editorial,we elaborate on the clinical guidelines,recent studies,and comparison of different endoscopic methods for the diagnosis of pancreatic cysts.展开更多
Powdery mildew negatively impacts wheat yield and quality.Emmer wheat(Triticum dicoccum),an ancestral species of common wheat,is a gene donor for wheat improvement.Cultivated emmer accession H1-707 exhibited all-stage...Powdery mildew negatively impacts wheat yield and quality.Emmer wheat(Triticum dicoccum),an ancestral species of common wheat,is a gene donor for wheat improvement.Cultivated emmer accession H1-707 exhibited all-stage resistance to powdery mildew over consecutive years.Genetic analysis of H1-707 at the seedling stage revealed a dominant monogenic inheritance pattern,and the underlying gene was designated Pm71.By employing bulked segregant exome sequencing(BSE-Seq)and using 2000 F2:3 families,Pm71 was fine mapped to a 336-kb interval on chromosome arm 6AS by referencing to the durum cv.Svevo RefSeq 1.0.Collinearity analysis revealed high homology in the candidate interval between Svevo and six Triticum species.Among six high-confidence genes annotated within this interval,TRITD6Av1G005050 encoding a GDSL esterase/lipase was identified as a key candidate for Pm71.展开更多
The introduction of wireless capsule endoscopy has brought a revolutionary change in the diagnostic procedures for gastrointestinal disorders.Biopsy,an essential procedure for disease diagnosis,has been integrated int...The introduction of wireless capsule endoscopy has brought a revolutionary change in the diagnostic procedures for gastrointestinal disorders.Biopsy,an essential procedure for disease diagnosis,has been integrated into robotic capsule endoscopy to augment diagnostic capabilities.In this study,we propose a magnetically driven biopsy robot based on a Kresling origami.Considering the bistable properties of Krelsing origami and the elasticity of the creases,a foldable structure of the robot with constant force characteristics is designed.The folding motion of the structure is used to deploy the needle into the target tissue.The robot is capable of performing rolling motion under the control of an external magnetic drive system,and a fine needle biopsy technique is used to collect deep tissue samples.We also conduct in vitro rolling experiments and sampling experiments on apple tissues and pork tissues,which verify the performance of the robot.展开更多
A new adsorbent was successfully prepared by hydrothermal treatment and chemical activation through coal gasification fine slag(CGFS)and blue algae(BA)as raw materials and used for CO_(2)capture.The CO_(2)chemisorptio...A new adsorbent was successfully prepared by hydrothermal treatment and chemical activation through coal gasification fine slag(CGFS)and blue algae(BA)as raw materials and used for CO_(2)capture.The CO_(2)chemisorption capacity of the adsorbent was further enhanced by taking advantage of the nitrogenous bases contained in the BA.In the hydrothermal process,the addition of BA significantly increased the content of pyrrole nitrogen in the adsorbent.In the activation process,pyrrole nitrogen gradually changed into pyridine nitrogen and graphite nitrogen.Increased BA addition result in a higher specific surface area and microporosity of the adsorbent.The CO_(2)adsorption performance test proved that the CGFS-50%-CA sample has the strongest CO_(2)adsorption capacity at low temperature,up to 15.59 cm^(3)/g,which is mainly through physical adsorption,and the CGFS-10%-CA sample has the strongest CO_(2)adsorption capacity at high temperature,up to 7.31 cm^(3)/g,which is mainly through chemical adsorption.CO_(2)uptake of the CGFS-10%-CA sample was well maintained after 10 cycles,with regeneration efficiencies above 99%.The results indicate that the novel adsorbents with coexistence of physical and chemical adsorption have great potential for CO_(2)adsorption applications.展开更多
Hanyu Xu 1,Xuedan Song 1,*,Qing Zhang 1,Chang Yu 1,Jieshan Qiu 1,2,*1 Liaoning Key Lab for Energy Materials and Chemical Engineering,State Key Laboratory of Fine Chemicals,School of Chemical Engineering,Dalian Univers...Hanyu Xu 1,Xuedan Song 1,*,Qing Zhang 1,Chang Yu 1,Jieshan Qiu 1,2,*1 Liaoning Key Lab for Energy Materials and Chemical Engineering,State Key Laboratory of Fine Chemicals,School of Chemical Engineering,Dalian University of Technology,Dalian 116024,Liaoning Province,China.展开更多
Increasing evidence suggests that fine roots are particularly sensitive to environmental changes,making them essential in responding and adapting forest ecosystems to climate change.However,we still lack a fundamental...Increasing evidence suggests that fine roots are particularly sensitive to environmental changes,making them essential in responding and adapting forest ecosystems to climate change.However,we still lack a fundamental understanding of the underlying mechanisms that control fine root plasticity.The objective of this study was to determine the influence of soil moisture changes on fine root dynamics and morphology of European beech(Fagus sylvatica L.).We conducted a 30-month study of fine root traits,i.e.,fine root biomass(FRB),productivity,mortality,turnover,specific root length(SRL),specific root area(SRA),and root tip frequency(RTF),along a soil moisture gradient from dry,intermediate,and wet conditions in a near-natural mature beech forest.Sequential root coring with accompanying soil measurements was carried out at three study sites reflecting the gradient in soil water availability.For most fine root traits,we found significant differences between the upper 10 cm and lower soil depths.FRB showed significant differences between study sites,with the lowest FRB at the dry site.However,productivity,turnover,SRL,SRA,and RTF showed no significant differences between sites,but a high variability between seasons,suggesting an adaptation to short-term fluctuations but not to long-term gradients in soil water content(SWC).Linear mixed models revealed that decreasing SWC led to a significant increase in SRL,SRA,and RTF(standardized coefficients:-1.0±0.46,-1.1±0.46,and-1.1±0.43,respectively).Our observations indicate an adaptation strategy of beech to low availability of soil water and drought by forming thin absorptive roots and by maintaining a high seasonal plasticity to tolerate fluctuations in soil moisture.By highlighting the belowground morphological adaptations of mature forests to low soil water availability,our results provide novel insights into the structure and dynamics of forest ecosystem adaptations to climate change.展开更多
Fine particulate matter(particulate matter with a diameter of 2.5μm or less;PM2.5)causes millions of premature deaths globally1,but not all particles are equally harmful2-4.Current air-pollution control strategies,pr...Fine particulate matter(particulate matter with a diameter of 2.5μm or less;PM2.5)causes millions of premature deaths globally1,but not all particles are equally harmful2-4.Current air-pollution control strategies,prioritizing PM2.5 mass reduction,have provided considerable health benefits but further refinements based on differences in the toxicity of various emission sources may provide greater benefits5-7.Here we integrated field measurements with air-quality modelling to assess the unequal toxicities of PM2.5 from various anthropogenic sources.展开更多
BACKGROUND Liver biopsy is an important diagnostic tool to investigate patients with suspected liver disease.The efficacy and safety of endoscopic ultrasound-guided liver biopsy(EUS-LB)as a method for liver tissue acq...BACKGROUND Liver biopsy is an important diagnostic tool to investigate patients with suspected liver disease.The efficacy and safety of endoscopic ultrasound-guided liver biopsy(EUS-LB)as a method for liver tissue acquisition remain uncertain.AIM To study the diagnostic yield and safety of EUS-LB in patients with suspected parenchymal liver disease.METHODS This is a retrospective observational cohort study of patients undergoing EUS-LB in 2 tertiary-care centers in Kuwait in the period between January 2022-June 2025.The primary outcome was sample adequacy,while secondary outcomes included histological quality of the specimen and adverse events.Predictors of increased diagnostic adequacy and specimen quality were explored.RESULTS A total of 50 patients[mean age 43.9(15.5)years,40%males]were included.The left liver lobe was targeted for biopsy in most patients(96%),and 19-gauge fine needle aspiration(FNA)was used in most cases(52%).The median number of passes performed was 3(range 2-5),and the heparin-wet suction technique was used in 40%of patients.The overall diagnostic adequacy of EUS-LB was 86%,with no significant difference between 19-gauge FNA and 19-gauge fine needle biopsy(FNB)needles.Fulfillment of the European Association for the Study of Liver(EASL)and the American Association for the Study of Liver Diseases criteria was achieved in 54%and 34%,respectively,with a significantly higher number of patients fulfilling these criteria when FNB needle was used compared to FNA needle(83.3%vs 26.9%,P<0.0001)and(62.5%vs 7.7%,P<0.0001),respectively.Only 1 patient(2.0%)developed adverse post-procedure events,namely bleeding requiring transfusion.Multivariate regression analysis did not identify any factor that increased diagnostic adequacy;however,the use of FNB needles and wet heparin suction methods were associated with significantly increased odds of fulfilling EASL criteria,while performing more than 2 passes reduced the quality of the specimen.CONCLUSION EUS-LB is an effective and safe method for obtaining liver biopsy in patients with suspected parenchymal liver disease.EUS-LB demonstrated a favorable safety profile in our cohort,although larger prospective studies are required to validate bleeding risk.The use of 19-gauge FNB needles,wet-heparin suction technique,and performing≤2 passes is associated with improved quality of the specimen obtained without increasing the risk of adverse events.展开更多
This study embarks on an explorative investigation into the effects of typical concentrations and varying particle sizes of fine grits(FG,the involatile portion of suspended solids)and fine debris(FD,the volatile yet ...This study embarks on an explorative investigation into the effects of typical concentrations and varying particle sizes of fine grits(FG,the involatile portion of suspended solids)and fine debris(FD,the volatile yet unbiodegradable fraction of suspended solids)within the influent on themixed liquor volatile suspended solids(MLVSS)/mixed liquor suspended solids(MLSS)ratio of an activated sludge system.Through meticulous experimentation,it was discerned that the addition of FG or FD,the particle size of FG,and the concentration of FD bore no substantial impact on the pollutant removal efficiency(denoted by the removal rate of COD and ammonia nitrogen)under constant operational conditions.However,a notable decrease in the MLVSS/MLSS ratio was observed with a typical FG concentration of 20 mg/L,with smaller FG particle sizes exacerbating this reduction.Additionally,variations in FD concentrations influenced both MLSS andMLVSS/MLSS ratios;a higher FD concentration led to an increased MLSS and a reduced MLVSS/MLSS ratio,indicating FD accumulation in the system.A predictive model for MLVSS/MLSS was constructed based on quality balance calculations,offering a tool for foreseeing the MLVSS/MLSS ratio under stable long-term influent conditions of FG and FD.This model,validated using data from the BXH wastewater treatment plant(WWTP),showcased remarkable accuracy.展开更多
Cattle carcass traits are economically important in the beef industry.In the present study,we identified 184 significant genes and 822 alternative genes for 7 carcass traits using genome-wide association studies(GWAS)...Cattle carcass traits are economically important in the beef industry.In the present study,we identified 184 significant genes and 822 alternative genes for 7 carcass traits using genome-wide association studies(GWAS)in 1,566 Huaxi beef cattle.We then identified 5,860 unique cis-genes and 734 trans-genes in 227 longissimus dorsi muscle(LDM)samples to better understand the genetic regulation of gene expression.Our integration study of the GWAS and cis-eQTL analysis detected 13 variants regulating 12 identical genes,in which one variant was also detected in fine-mapping analysis.Moreover,using a transcriptome-wide association study(TWAS),we identified 4 genes(TTC30B,HMGA1,PRKD3 and FXN)that were significantly related to carcass chest depth(CCD),carcass length(CL),carcass weight(CW)and dressing percentage(DP).This study identified variants and genes that may be useful for understanding the molecular mechanism of carcass traits in beef cattle.展开更多
Rubbery waste at the end of the cycle often constitutes a threat for the environment because of their encumbrance and low biodeterioration.The purpose of the research presented is to develop the rubber fine powder as ...Rubbery waste at the end of the cycle often constitutes a threat for the environment because of their encumbrance and low biodeterioration.The purpose of the research presented is to develop the rubber fine powder as a pavement.It is interested primarily in the behavior of two types of bitumen 40/50 modified by the addition of two varieties of rubber fine powders of different grading,resulting from the crushing of the rubbery products intended for the clothes industry of soles of shoes.The objective of the experimentation is to study the influence of the added polymer on the physical properties of the ordinary road bitumen with the incorporation of the fine powder.The experimental approach is carried out using the two tests of characterization of the bitumen i.e.the softening point test and the penetration test which remain the most used to define and classify the road bitumen.It will be noted however,that the experimental investigation which is based on several tests according to the type and the content of fine powders,leads on a whole of interesting correlations.展开更多
Melon is a globally cultivated horticultural crop with a predominantly hybrid commercial seed market in China.Seedling morphology,particularly hypocotyl color,is a valuable trait for rapid F1 hybrid seed purity assess...Melon is a globally cultivated horticultural crop with a predominantly hybrid commercial seed market in China.Seedling morphology,particularly hypocotyl color,is a valuable trait for rapid F1 hybrid seed purity assessment.While green hypocotyls are common,white hypocotyls are rare in melon germplasm.This study identified a mutant with white hypocotyl but green leaves from the heavy ion beam mutant library.Genetic analysis revealed that a single recessive gene controlled the white hypocotyl,designated CmGhc1.A single-base deletion in the fifth exon of CmGhc1 led to a truncated CmGhc1 lacking the HTH-MYB DNA binding domain,likely affecting its transcriptional activity.CmGhc1 was localized in the nucleus,and yeast two-hybrid analysis and a dual-LUC assay demonstrated it as a transcription repressor.Furthermore,a KASP marker(hc1)was developed and verified as a functional marker for breeding white hypocotyl germplasms in melon.RNA-seq data revealed that CmGhc1 significantly affected the transcription of genes related to chlorophyll metabolism and photosynthesis in hypocotyl.In summary,these findings contribute to our understanding of chloroplast biogenesis and provide a valuable tool for melon breeding.展开更多
Peanut is a globally significant oil crop and economic resource,notable for its kernel containing over 50%oil content.White testa peanuts are highly valued for their superior nutritional profile,minimal pigmentation,a...Peanut is a globally significant oil crop and economic resource,notable for its kernel containing over 50%oil content.White testa peanuts are highly valued for their superior nutritional profile,minimal pigmentation,and superior oil clarity.Identification of genes controlling white testa color is crucial for advancing breeding programs and understanding the genetic mechanisms involved.A genetic mapping study was performed in peanut to identify genes controlling white testa color,a trait associated with desirable end-use quality traits in this oilseed crop.In an F_(2)population generated from a cross of a white-testa with a pink-testa cultivar,two recessive quantitative-trait loci controlling white testa were identified and finemapped to A02 and B02 chromosomes.Two homologous genes,Arahy.MP3D3D and Arahy.26781N,encoding bHLH transcriptional factors,were identified as candidates for the two loci.Reduced expression of these two genes likely suppresses anthocyanin biosynthesis.展开更多
Fine debris is an important component of natural debris flows.Previous studies focused primarily on the clay minerals found in the fines,and non-clay minerals were often neglected.The effects of mineralogy of fines on...Fine debris is an important component of natural debris flows.Previous studies focused primarily on the clay minerals found in the fines,and non-clay minerals were often neglected.The effects of mineralogy of fines on debris-mass slurrying and flow behaviors of the resultant slurries are examined herein.The fines(≤0.04 mm)in the<5 mm fraction of the Dongyuege Creek debris-flow deposit is replaced with five other mineral powders with the same maximum particle size.Four types of separate and sequential experiments related to debris slurrying and slurry behaviors are carried out with the prepared clastic materials.The obtained slurrying index ranging from 0.08 to 0.18 shows that non-clay minerals also can function as the fine fractions of debris-flow materials,so long as the requirement of grain size distribution is met.Equidimensional,non-clay minerals making up fines of debris flows can increase the upper solid concentration limits of slurrying(with a maximum of 0.692)and peak values of relative excess water pressure(measured maximum mean peak value is 0.99),leading to higher momentum and higher competence,and thereby more destructive catastrophe.The sediments with platy non-clay mineral-dominated fines have potential for mobilizing into small-to medium-size debris flows with a relatively small competence.Clay minerals in the fines may indeed enhance the liquefaction potential of debris masses by expanding the difference between upper and lower solid concentration limits of slurrying(0.413 and 0.238,respectively,for pure kaolinite),but they significantly suppress the momentum,competence,and destructive power of potential debris flows by lowering upper solid concentration limit of slurrying of debris masses.Alpine catchments rich in non-clay minerals,notably those releasing dolomite into loose sediments,may be more prone to threatening and destructive debris flows.The basin producing clay minerals should be more susceptible to lowmagnitude/high-frequency debris flows with less devastating consequences.展开更多
The mineral composition of the sinter affects the quality of cold-bonded briquettes(CBB),which are prepared from returned sinter fines and serve as a cleaner blast furnace charge.Pulverization rate,compressive strengt...The mineral composition of the sinter affects the quality of cold-bonded briquettes(CBB),which are prepared from returned sinter fines and serve as a cleaner blast furnace charge.Pulverization rate,compressive strength,reduction disintegration index(RDI)and compressive strength after reduction experiment were tested to analyze the experimental parameters of CBB under the influence of different basicities and sintering time.The results show that when the basicity of CBB is increased from 0.5 to 1.5,the pulverization rate increases,and performance indexes such as compressive strength,RDI,and compressive strength exhibit a decreasing trend.When the basicity is increased from 1.5 to 3.0,all the aforementioned performance indexes are improved.When the sintering time is extended from 0 to 8 h,the properties mentioned above are improved.The results of X-ray diffraction,microstructure,and thermodynamic calculations confirm that the hematite in the mineral composition of CBB tends to convert into calcium ferrite,which leads to the increased compressive strength of CBB.The reasonable basicity and sintering time during sinter preparation not only form the desired mineral composition but also improve the properties of the CBB.展开更多
Soybean seed isoflavones are a type of secondary metabolites that can provide health and nutrition benefits for humans. In our previous study, a stable quantitative trait locus(QTL) qIF05-1 controlling the seed isofla...Soybean seed isoflavones are a type of secondary metabolites that can provide health and nutrition benefits for humans. In our previous study, a stable quantitative trait locus(QTL) qIF05-1 controlling the seed isoflavone content in soybean was detected on chromosome(Chr.) 05 in a recombinant inbred line(RIL) population from a cross of Huachun 2×Wayao. In this study, the parental lines were re-sequenced using the Illumina Solexa System with deep coverage. A total of 63,099 polymorphic long insertions and deletions(InDels)(≥15 bp)were identified between the parents Huachun 2 and Wayao. The InDels were unevenly distributed on 20chromosomes of soybean, varying from 1,826 in Chr. 12 to 4,544 in Chr. 18. A total of 10,002 long InDels(15.85% of total) were located in genic regions, including 1,139 large-effect long InDels which resulted in truncated or elongated protein sequences. In the qIF05-1 region, 68 long InDels were detected between the two parents. Using a progeny recombination experiment and genotype analysis, the qIF05-1 locus was mapped into a 102.2 kb genomic region, and this region contained 12 genes. By RNA-seq data analysis, genome sequence comparison and functional validation through ectopic expression in Arabidopsis thaliana, Glyma.05G208300(described as GmEGL3), which is a basic helix-loop-helix(bHLH) transcription factor in plants, emerged as the most likely confirmed gene in qIF05-1. These long InDels can be used as a type of complementary genetic method for QTL fine mapping, and they can facilitate genetic studies and molecular-assisted selection breeding in soybean.展开更多
This paper aims to develop a nonrigid registration method of preoperative and intraoperative thoracoabdominal CT images in computer-assisted interventional surgeries for accurate tumor localization and tissue visualiz...This paper aims to develop a nonrigid registration method of preoperative and intraoperative thoracoabdominal CT images in computer-assisted interventional surgeries for accurate tumor localization and tissue visualization enhancement.However,fine structure registration of complex thoracoabdominal organs and large deformation registration caused by respiratory motion is challenging.To deal with this problem,we propose a 3D multi-scale attention VoxelMorph(MAVoxelMorph)registration network.To alleviate the large deformation problem,a multi-scale axial attention mechanism is utilized by using a residual dilated pyramid pooling for multi-scale feature extraction,and position-aware axial attention for long-distance dependencies between pixels capture.To further improve the large deformation and fine structure registration results,a multi-scale context channel attention mechanism is employed utilizing content information via adjacent encoding layers.Our method was evaluated on four public lung datasets(DIR-Lab dataset,Creatis dataset,Learn2Reg dataset,OASIS dataset)and a local dataset.Results proved that the proposed method achieved better registration performance than current state-of-the-art methods,especially in handling the registration of large deformations and fine structures.It also proved to be fast in 3D image registration,using about 1.5 s,and faster than most methods.Qualitative and quantitative assessments proved that the proposed MA-VoxelMorph has the potential to realize precise and fast tumor localization in clinical interventional surgeries.展开更多
基金Project（2016zztsl03） supported by the Fundamental Research Funds for the Central Universities, China Project（51374249） supported by the National Natural Science Foundation of China+1 种基金 Project（2015BAB12B02） supported by the National Key Technology R＆D Program of China Project（2013B090800016） supported by Guangdong Provincial Science and Technology Plan, China
文摘An effective flotation approach is proposed for improving the recovery of molybdenite fines from a finely-disseminated molybdenum ore. To maximize the flotation recovery of molybdenum, process mineralogy of raw ore, contrast tests, optimization of operation conditions and particle size analysis were systematically investigated. Process mineralogy suggests that in the raw ore, 61.63% of molybdenite particles distribute in the 〈20 pm size fraction, and intergrow with muscovite and pyrite as the contained and disseminated type. Contrast tests indicate that conventional flotation responds to poor collection efficiency for particles less than 25 pm. Oil agglomerate flotation （OAF） process demonstrates an obvious superiority in improving the flotation recovery of molybdenite fines. Furthermore, the flotation results of OAF process reveal that the dosage of transformer oil plays a critical role on the average size of collected mineral particles （d（0）, agglomerates （d^0） and the molybdenum recovery. In addition, industrial tests illustrate that compared with the Mo-S bulk flotation approach, OAF process not only increases Mo recovery and grade of molybdenum concentrate by 22.75% and 17.47% respectively, but also achieves a sulfur concentrate with a superior grade of 38.92%.
基金This study was financially supported by the National Key Research and Development Program of China(Project No:2021YFD1300202)the National Natural Science Foundation of China(Project No:32102587).
文摘We investigated the effects of finely ground wheat bran on the nutrient digestibility, digesta passage rate,and gut microbiota structure in sows. A 3 × 3 Latin square design with 3 test periods and 3 experimental diets was used. Six non-pregnant sows(parity: 5 to 7) were randomly assigned to 3 experimental diets with 2 replicates per treatment in each period. Each period lasted 19 d(12 d for adaptation and 7 d for experiment). The experimental diets included(a) a basal corn and soybean meal diet(CON),(b) a basal diet with 20% coarse wheat bran(CWB;particle size: 605 μm), and(c) a basal diet with 20% fine wheat bran(FWB;particle size: 438 μm). The results demonstrated that the apparent total tract digestibility of neutral detergent fiber, acid detergent fiber and energy were reduced(P < 0.05) in the FWB and CWB groups compared with those in the CON group. Viscosity of digesta increased(P < 0.001) in FWB-fed sows. The passage rate of digesta from the mouth to the ileum decreased(P < 0.001) in FWB-fed sows. Peptide YY(PYY) concentration increased(P = 0.01) in FWB-fed sows after 30 min of feeding.In the FWB group, the relative abundance of Lactobacillaceae at the family level increased(P < 0.05) in the ileal digesta. At the class level, the relative abundance of Clostridia in feces decreased(P < 0.05) in FWB-fed sows. FWB enhanced the concentration of butyrate in feces compared with CON and CWB(P = 0.04). These results suggest that dietary supplementation with finely ground wheat bran reduces the passage rate of digesta, increases the abundance of beneficial microorganisms, and elevates the concentration of short-chain fatty acids and PYY in sows. These findings indicate that the addition of finelyground wheat bran to the diets of sows is more effective than using coarse wheat bran for improving their satiety and intestinal microbial composition.
文摘Particle coatings are used extensively to generate dispersed solids with well-defined properties, e.g., to protect active ingredients, with most coating processes using core particles of a diameter larger than 200 μm. This work contributes to the development of a coating process for fine dispersed particles （diam- eter less than 50 μm） by combining two particle-formulation processes, namely, coating and spray drying. The feasibility of the operation is based on and demonstrated by the innovative application of a two-fluid nozzle. Experiments were conducted by using glass particles as core particles and sodium benzoate as the coating agent. The coating of finely dispersed particles is achieved by the spraying of particles and coating solution as a homogeneous suspension. The aim is to create droplets with only one contained particle at the nozzle outlet. After evaporation of the water in the droplet, a thin solid film is built on the particle surface. The suspension viscosity was measured and compared with empirical equations from the literature. The liquid-film thickness on the particle surface was calculated to predict the building of a uniform coating layer or agglomerates. In this study, the feasibility of pneumatic transport through the nozzle and an investigation of the process were illustrated. The agglomeration fraction and degree of coating of the particle surface were analyzed optically by scanning electron microscopy. In this way, the influence of different processes and suspension parameters on the product quality were determined.
文摘Pancreatic cysts are mostly incidental findings on computed tomography or magnetic resonance imaging scans,with few patients presenting with abdominal pain or other symptoms.The accurate diagnosis of cysts is important as management depends on the type(neoplastic or non-neoplastic).Cross-sectional imaging is fast being replaced with endoscopic ultrasound(EUS)and various techniques based on that such as EUS-guided fine needle aspiration,EUS-guided needle confocal laser endomicroscopy,EUS-through-the-needle biopsy,and contrast-enhanced EUS.Clinical studies have reported varying diagnostic and adverse event rates with these modalities.In addition,American,European,and Kyoto guidelines for the diagnosis and management of pancreatic cysts have provided different recommendations.In this editorial,we elaborate on the clinical guidelines,recent studies,and comparison of different endoscopic methods for the diagnosis of pancreatic cysts.
基金financially supported by National Natural Science Foundation of China(32301800,32301923 and 32072053)Wheat Industrial Technology System of Shandong Province(SDAIT-01-01)Key Research and Development Project of Shandong Province(2022LZG002-4,2023LZGC009-4-4).
文摘Powdery mildew negatively impacts wheat yield and quality.Emmer wheat(Triticum dicoccum),an ancestral species of common wheat,is a gene donor for wheat improvement.Cultivated emmer accession H1-707 exhibited all-stage resistance to powdery mildew over consecutive years.Genetic analysis of H1-707 at the seedling stage revealed a dominant monogenic inheritance pattern,and the underlying gene was designated Pm71.By employing bulked segregant exome sequencing(BSE-Seq)and using 2000 F2:3 families,Pm71 was fine mapped to a 336-kb interval on chromosome arm 6AS by referencing to the durum cv.Svevo RefSeq 1.0.Collinearity analysis revealed high homology in the candidate interval between Svevo and six Triticum species.Among six high-confidence genes annotated within this interval,TRITD6Av1G005050 encoding a GDSL esterase/lipase was identified as a key candidate for Pm71.
基金supported by the National Natural Science Foundation of China(Grant Nos.51805047 and 52175003)the Outstanding Youth Program of Hunan Education Department(Grant No.23B0335)the Natural Science Foundation of Hunan Province(Grant Nos.2023JJ30021 and 2023JJ50077).
文摘The introduction of wireless capsule endoscopy has brought a revolutionary change in the diagnostic procedures for gastrointestinal disorders.Biopsy,an essential procedure for disease diagnosis,has been integrated into robotic capsule endoscopy to augment diagnostic capabilities.In this study,we propose a magnetically driven biopsy robot based on a Kresling origami.Considering the bistable properties of Krelsing origami and the elasticity of the creases,a foldable structure of the robot with constant force characteristics is designed.The folding motion of the structure is used to deploy the needle into the target tissue.The robot is capable of performing rolling motion under the control of an external magnetic drive system,and a fine needle biopsy technique is used to collect deep tissue samples.We also conduct in vitro rolling experiments and sampling experiments on apple tissues and pork tissues,which verify the performance of the robot.
基金supported by the National Natural Science Foundation of China(22168032)the National Key Research and Development Program of China(2023YFC3904302,2023YFB4103500)the Key Projects of Ning Dong Energy and Chemical Industry Base(2023NDKJXMLX022).
文摘A new adsorbent was successfully prepared by hydrothermal treatment and chemical activation through coal gasification fine slag(CGFS)and blue algae(BA)as raw materials and used for CO_(2)capture.The CO_(2)chemisorption capacity of the adsorbent was further enhanced by taking advantage of the nitrogenous bases contained in the BA.In the hydrothermal process,the addition of BA significantly increased the content of pyrrole nitrogen in the adsorbent.In the activation process,pyrrole nitrogen gradually changed into pyridine nitrogen and graphite nitrogen.Increased BA addition result in a higher specific surface area and microporosity of the adsorbent.The CO_(2)adsorption performance test proved that the CGFS-50%-CA sample has the strongest CO_(2)adsorption capacity at low temperature,up to 15.59 cm^(3)/g,which is mainly through physical adsorption,and the CGFS-10%-CA sample has the strongest CO_(2)adsorption capacity at high temperature,up to 7.31 cm^(3)/g,which is mainly through chemical adsorption.CO_(2)uptake of the CGFS-10%-CA sample was well maintained after 10 cycles,with regeneration efficiencies above 99%.The results indicate that the novel adsorbents with coexistence of physical and chemical adsorption have great potential for CO_(2)adsorption applications.
文摘Hanyu Xu 1,Xuedan Song 1,*,Qing Zhang 1,Chang Yu 1,Jieshan Qiu 1,2,*1 Liaoning Key Lab for Energy Materials and Chemical Engineering,State Key Laboratory of Fine Chemicals,School of Chemical Engineering,Dalian University of Technology,Dalian 116024,Liaoning Province,China.
基金supported by the Federal Ministry of Food and Agriculture (BMEL)the Federal Ministry for the Environment,Nature Conservation,Nuclear Safety and Consumer Protection (BMUV) through the Fachagentur Nachwachsende Rohstoffe e. V.(FNR)(grant no. 2218 WK53X4)
文摘Increasing evidence suggests that fine roots are particularly sensitive to environmental changes,making them essential in responding and adapting forest ecosystems to climate change.However,we still lack a fundamental understanding of the underlying mechanisms that control fine root plasticity.The objective of this study was to determine the influence of soil moisture changes on fine root dynamics and morphology of European beech(Fagus sylvatica L.).We conducted a 30-month study of fine root traits,i.e.,fine root biomass(FRB),productivity,mortality,turnover,specific root length(SRL),specific root area(SRA),and root tip frequency(RTF),along a soil moisture gradient from dry,intermediate,and wet conditions in a near-natural mature beech forest.Sequential root coring with accompanying soil measurements was carried out at three study sites reflecting the gradient in soil water availability.For most fine root traits,we found significant differences between the upper 10 cm and lower soil depths.FRB showed significant differences between study sites,with the lowest FRB at the dry site.However,productivity,turnover,SRL,SRA,and RTF showed no significant differences between sites,but a high variability between seasons,suggesting an adaptation to short-term fluctuations but not to long-term gradients in soil water content(SWC).Linear mixed models revealed that decreasing SWC led to a significant increase in SRL,SRA,and RTF(standardized coefficients:-1.0±0.46,-1.1±0.46,and-1.1±0.43,respectively).Our observations indicate an adaptation strategy of beech to low availability of soil water and drought by forming thin absorptive roots and by maintaining a high seasonal plasticity to tolerate fluctuations in soil moisture.By highlighting the belowground morphological adaptations of mature forests to low soil water availability,our results provide novel insights into the structure and dynamics of forest ecosystem adaptations to climate change.
文摘Fine particulate matter(particulate matter with a diameter of 2.5μm or less;PM2.5)causes millions of premature deaths globally1,but not all particles are equally harmful2-4.Current air-pollution control strategies,prioritizing PM2.5 mass reduction,have provided considerable health benefits but further refinements based on differences in the toxicity of various emission sources may provide greater benefits5-7.Here we integrated field measurements with air-quality modelling to assess the unequal toxicities of PM2.5 from various anthropogenic sources.
文摘BACKGROUND Liver biopsy is an important diagnostic tool to investigate patients with suspected liver disease.The efficacy and safety of endoscopic ultrasound-guided liver biopsy(EUS-LB)as a method for liver tissue acquisition remain uncertain.AIM To study the diagnostic yield and safety of EUS-LB in patients with suspected parenchymal liver disease.METHODS This is a retrospective observational cohort study of patients undergoing EUS-LB in 2 tertiary-care centers in Kuwait in the period between January 2022-June 2025.The primary outcome was sample adequacy,while secondary outcomes included histological quality of the specimen and adverse events.Predictors of increased diagnostic adequacy and specimen quality were explored.RESULTS A total of 50 patients[mean age 43.9(15.5)years,40%males]were included.The left liver lobe was targeted for biopsy in most patients(96%),and 19-gauge fine needle aspiration(FNA)was used in most cases(52%).The median number of passes performed was 3(range 2-5),and the heparin-wet suction technique was used in 40%of patients.The overall diagnostic adequacy of EUS-LB was 86%,with no significant difference between 19-gauge FNA and 19-gauge fine needle biopsy(FNB)needles.Fulfillment of the European Association for the Study of Liver(EASL)and the American Association for the Study of Liver Diseases criteria was achieved in 54%and 34%,respectively,with a significantly higher number of patients fulfilling these criteria when FNB needle was used compared to FNA needle(83.3%vs 26.9%,P<0.0001)and(62.5%vs 7.7%,P<0.0001),respectively.Only 1 patient(2.0%)developed adverse post-procedure events,namely bleeding requiring transfusion.Multivariate regression analysis did not identify any factor that increased diagnostic adequacy;however,the use of FNB needles and wet heparin suction methods were associated with significantly increased odds of fulfilling EASL criteria,while performing more than 2 passes reduced the quality of the specimen.CONCLUSION EUS-LB is an effective and safe method for obtaining liver biopsy in patients with suspected parenchymal liver disease.EUS-LB demonstrated a favorable safety profile in our cohort,although larger prospective studies are required to validate bleeding risk.The use of 19-gauge FNB needles,wet-heparin suction technique,and performing≤2 passes is associated with improved quality of the specimen obtained without increasing the risk of adverse events.
基金supported by the National Special Project for Science and Technology on Water Pollution Control and Management(No.2017ZX07102-003)the Fundamental Research Funds for the Central Universities,and the Research Funds of Renmin University of China(No.2020030257).
文摘This study embarks on an explorative investigation into the effects of typical concentrations and varying particle sizes of fine grits(FG,the involatile portion of suspended solids)and fine debris(FD,the volatile yet unbiodegradable fraction of suspended solids)within the influent on themixed liquor volatile suspended solids(MLVSS)/mixed liquor suspended solids(MLSS)ratio of an activated sludge system.Through meticulous experimentation,it was discerned that the addition of FG or FD,the particle size of FG,and the concentration of FD bore no substantial impact on the pollutant removal efficiency(denoted by the removal rate of COD and ammonia nitrogen)under constant operational conditions.However,a notable decrease in the MLVSS/MLSS ratio was observed with a typical FG concentration of 20 mg/L,with smaller FG particle sizes exacerbating this reduction.Additionally,variations in FD concentrations influenced both MLSS andMLVSS/MLSS ratios;a higher FD concentration led to an increased MLSS and a reduced MLVSS/MLSS ratio,indicating FD accumulation in the system.A predictive model for MLVSS/MLSS was constructed based on quality balance calculations,offering a tool for foreseeing the MLVSS/MLSS ratio under stable long-term influent conditions of FG and FD.This model,validated using data from the BXH wastewater treatment plant(WWTP),showcased remarkable accuracy.
基金supported by the National Natural Science Foundation of China(32372833 and 32202652)the Agricultural Science and Technology Innovation Program in the Chinese Academy of Agricultural Sciences(ASTIP-IAS03 and CAASZDRW202102)+1 种基金the Program of National Beef Cattle and Yak Industrial Technology System,China(CARS-37)the Science and Technology Project of Inner Mongolia Autonomus Region,China(2020GG0210)。
文摘Cattle carcass traits are economically important in the beef industry.In the present study,we identified 184 significant genes and 822 alternative genes for 7 carcass traits using genome-wide association studies(GWAS)in 1,566 Huaxi beef cattle.We then identified 5,860 unique cis-genes and 734 trans-genes in 227 longissimus dorsi muscle(LDM)samples to better understand the genetic regulation of gene expression.Our integration study of the GWAS and cis-eQTL analysis detected 13 variants regulating 12 identical genes,in which one variant was also detected in fine-mapping analysis.Moreover,using a transcriptome-wide association study(TWAS),we identified 4 genes(TTC30B,HMGA1,PRKD3 and FXN)that were significantly related to carcass chest depth(CCD),carcass length(CL),carcass weight(CW)and dressing percentage(DP).This study identified variants and genes that may be useful for understanding the molecular mechanism of carcass traits in beef cattle.
文摘Rubbery waste at the end of the cycle often constitutes a threat for the environment because of their encumbrance and low biodeterioration.The purpose of the research presented is to develop the rubber fine powder as a pavement.It is interested primarily in the behavior of two types of bitumen 40/50 modified by the addition of two varieties of rubber fine powders of different grading,resulting from the crushing of the rubbery products intended for the clothes industry of soles of shoes.The objective of the experimentation is to study the influence of the added polymer on the physical properties of the ordinary road bitumen with the incorporation of the fine powder.The experimental approach is carried out using the two tests of characterization of the bitumen i.e.the softening point test and the penetration test which remain the most used to define and classify the road bitumen.It will be noted however,that the experimental investigation which is based on several tests according to the type and the content of fine powders,leads on a whole of interesting correlations.
基金financially supported by the grants from the earmarked fund for Xinjiang Agriculture Research System,China(XJARS-06)the Key Research and Development Task Special Project of Xinjiang,China(2022B02002-3)+1 种基金the Tianshan Talent Training Program,China(2023TSYCLJ0015)the Key Research and Development Program of Xinjiang Uygur Autonomous Region,China(2023B02017).
文摘Melon is a globally cultivated horticultural crop with a predominantly hybrid commercial seed market in China.Seedling morphology,particularly hypocotyl color,is a valuable trait for rapid F1 hybrid seed purity assessment.While green hypocotyls are common,white hypocotyls are rare in melon germplasm.This study identified a mutant with white hypocotyl but green leaves from the heavy ion beam mutant library.Genetic analysis revealed that a single recessive gene controlled the white hypocotyl,designated CmGhc1.A single-base deletion in the fifth exon of CmGhc1 led to a truncated CmGhc1 lacking the HTH-MYB DNA binding domain,likely affecting its transcriptional activity.CmGhc1 was localized in the nucleus,and yeast two-hybrid analysis and a dual-LUC assay demonstrated it as a transcription repressor.Furthermore,a KASP marker(hc1)was developed and verified as a functional marker for breeding white hypocotyl germplasms in melon.RNA-seq data revealed that CmGhc1 significantly affected the transcription of genes related to chlorophyll metabolism and photosynthesis in hypocotyl.In summary,these findings contribute to our understanding of chloroplast biogenesis and provide a valuable tool for melon breeding.
基金supported by the Key Research and Development Program of China(2022YFD1200400)National Natural Science Foundation of China(32472041)+2 种基金the Project of the Development for High-quality Seed Industry of Hubei Province(HBZY2023B003)Key Research and Development Program of Hubei Province(2021BBA077)Innovation Program of the Chinese Academy of Agricultural Sciences(2024-2060299-089-031)。
文摘Peanut is a globally significant oil crop and economic resource,notable for its kernel containing over 50%oil content.White testa peanuts are highly valued for their superior nutritional profile,minimal pigmentation,and superior oil clarity.Identification of genes controlling white testa color is crucial for advancing breeding programs and understanding the genetic mechanisms involved.A genetic mapping study was performed in peanut to identify genes controlling white testa color,a trait associated with desirable end-use quality traits in this oilseed crop.In an F_(2)population generated from a cross of a white-testa with a pink-testa cultivar,two recessive quantitative-trait loci controlling white testa were identified and finemapped to A02 and B02 chromosomes.Two homologous genes,Arahy.MP3D3D and Arahy.26781N,encoding bHLH transcriptional factors,were identified as candidates for the two loci.Reduced expression of these two genes likely suppresses anthocyanin biosynthesis.
基金supported by the National Natural Science Foundation of China(41931294)the National Natural Science Foundation of China-Yunnan Joint Fund(U1502232,U1033601)。
文摘Fine debris is an important component of natural debris flows.Previous studies focused primarily on the clay minerals found in the fines,and non-clay minerals were often neglected.The effects of mineralogy of fines on debris-mass slurrying and flow behaviors of the resultant slurries are examined herein.The fines(≤0.04 mm)in the<5 mm fraction of the Dongyuege Creek debris-flow deposit is replaced with five other mineral powders with the same maximum particle size.Four types of separate and sequential experiments related to debris slurrying and slurry behaviors are carried out with the prepared clastic materials.The obtained slurrying index ranging from 0.08 to 0.18 shows that non-clay minerals also can function as the fine fractions of debris-flow materials,so long as the requirement of grain size distribution is met.Equidimensional,non-clay minerals making up fines of debris flows can increase the upper solid concentration limits of slurrying(with a maximum of 0.692)and peak values of relative excess water pressure(measured maximum mean peak value is 0.99),leading to higher momentum and higher competence,and thereby more destructive catastrophe.The sediments with platy non-clay mineral-dominated fines have potential for mobilizing into small-to medium-size debris flows with a relatively small competence.Clay minerals in the fines may indeed enhance the liquefaction potential of debris masses by expanding the difference between upper and lower solid concentration limits of slurrying(0.413 and 0.238,respectively,for pure kaolinite),but they significantly suppress the momentum,competence,and destructive power of potential debris flows by lowering upper solid concentration limit of slurrying of debris masses.Alpine catchments rich in non-clay minerals,notably those releasing dolomite into loose sediments,may be more prone to threatening and destructive debris flows.The basin producing clay minerals should be more susceptible to lowmagnitude/high-frequency debris flows with less devastating consequences.
基金supported by Distinguished Professor(Tracking Plan)of Oriental Scholars of Shanghai Universities(Grant No.GZ2020013)National 111 Project(The Program of Introducing Talents of Discipline to University)(Grant No.Dl7002)+1 种基金Independent Research Project of State Key Laboratory of Advanced Special Steel Shanghai Key Laboratory of Advanced Ferrometallurgy and Shanghai University(Grant No.SKLASS 2022-Z011)Additionally,the authors are appreciative of the funding from the Academy of Finland with Grant No.349833.
文摘The mineral composition of the sinter affects the quality of cold-bonded briquettes(CBB),which are prepared from returned sinter fines and serve as a cleaner blast furnace charge.Pulverization rate,compressive strength,reduction disintegration index(RDI)and compressive strength after reduction experiment were tested to analyze the experimental parameters of CBB under the influence of different basicities and sintering time.The results show that when the basicity of CBB is increased from 0.5 to 1.5,the pulverization rate increases,and performance indexes such as compressive strength,RDI,and compressive strength exhibit a decreasing trend.When the basicity is increased from 1.5 to 3.0,all the aforementioned performance indexes are improved.When the sintering time is extended from 0 to 8 h,the properties mentioned above are improved.The results of X-ray diffraction,microstructure,and thermodynamic calculations confirm that the hematite in the mineral composition of CBB tends to convert into calcium ferrite,which leads to the increased compressive strength of CBB.The reasonable basicity and sintering time during sinter preparation not only form the desired mineral composition but also improve the properties of the CBB.
基金supported by the China Agriculture Research System of MOF and MARA(CARS-04-PS12)the Research and Development Program in the Key-Areas of Guangdong Province,China(2020B020220008)the Guangdong Agricultural Research System,China(2023KJ136-03).
文摘Soybean seed isoflavones are a type of secondary metabolites that can provide health and nutrition benefits for humans. In our previous study, a stable quantitative trait locus(QTL) qIF05-1 controlling the seed isoflavone content in soybean was detected on chromosome(Chr.) 05 in a recombinant inbred line(RIL) population from a cross of Huachun 2×Wayao. In this study, the parental lines were re-sequenced using the Illumina Solexa System with deep coverage. A total of 63,099 polymorphic long insertions and deletions(InDels)(≥15 bp)were identified between the parents Huachun 2 and Wayao. The InDels were unevenly distributed on 20chromosomes of soybean, varying from 1,826 in Chr. 12 to 4,544 in Chr. 18. A total of 10,002 long InDels(15.85% of total) were located in genic regions, including 1,139 large-effect long InDels which resulted in truncated or elongated protein sequences. In the qIF05-1 region, 68 long InDels were detected between the two parents. Using a progeny recombination experiment and genotype analysis, the qIF05-1 locus was mapped into a 102.2 kb genomic region, and this region contained 12 genes. By RNA-seq data analysis, genome sequence comparison and functional validation through ectopic expression in Arabidopsis thaliana, Glyma.05G208300(described as GmEGL3), which is a basic helix-loop-helix(bHLH) transcription factor in plants, emerged as the most likely confirmed gene in qIF05-1. These long InDels can be used as a type of complementary genetic method for QTL fine mapping, and they can facilitate genetic studies and molecular-assisted selection breeding in soybean.
基金supported in part by the National Natural Science Foundation of China[62301374]Hubei Provincial Natural Science Foundation of China[2022CFB804]+2 种基金Hubei Provincial Education Research Project[B2022057]the Youths Science Foundation of Wuhan Institute of Technology[K202240]the 15th Graduate Education Innovation Fund of Wuhan Institute of Technology[CX2023295].
文摘This paper aims to develop a nonrigid registration method of preoperative and intraoperative thoracoabdominal CT images in computer-assisted interventional surgeries for accurate tumor localization and tissue visualization enhancement.However,fine structure registration of complex thoracoabdominal organs and large deformation registration caused by respiratory motion is challenging.To deal with this problem,we propose a 3D multi-scale attention VoxelMorph(MAVoxelMorph)registration network.To alleviate the large deformation problem,a multi-scale axial attention mechanism is utilized by using a residual dilated pyramid pooling for multi-scale feature extraction,and position-aware axial attention for long-distance dependencies between pixels capture.To further improve the large deformation and fine structure registration results,a multi-scale context channel attention mechanism is employed utilizing content information via adjacent encoding layers.Our method was evaluated on four public lung datasets(DIR-Lab dataset,Creatis dataset,Learn2Reg dataset,OASIS dataset)and a local dataset.Results proved that the proposed method achieved better registration performance than current state-of-the-art methods,especially in handling the registration of large deformations and fine structures.It also proved to be fast in 3D image registration,using about 1.5 s,and faster than most methods.Qualitative and quantitative assessments proved that the proposed MA-VoxelMorph has the potential to realize precise and fast tumor localization in clinical interventional surgeries.
