The research on the application of X-ray diffraction in the quantitative analysis of Chinese medicines is rare. The main reason is that the technical problems related to the internal standard and the selection of quan...The research on the application of X-ray diffraction in the quantitative analysis of Chinese medicines is rare. The main reason is that the technical problems related to the internal standard and the selection of quantitative peaks are not well solved, and the accuracy and precision of the results are not satisfactory. This study employed the concept of mass absorption coefficient based on the internal standard method, and the full spectrum fitting and quantitative methods were used to solve the above technical problems. The sample was blended. the internal standard substance of zinc oxide was fully ground, and tablets were prepared by positive pressure method. Under certain instrumental conditions, the PXRD pattern was obtained by scanning. The percentage of gypsum fibrosum in Xiaokening tablet was obtained by quantitative analysis of full spectrum fitting internal standard by TOPAS software. The method was investigated by methodology. At the same time, the method was compared by ion chromatography, and SPSS software was used to make a significant t test on the results of the two methods. After the investigation, the average standard recovery rate of CaSO4-2H2O was 99.06%(RSD = 3.02%);and the recovery rate for simulated samples was 96.7%. The method had good specificity. After statistical analysis, there was no significant difference between the new PXRD method and the traditional method of ion chromatography.展开更多
Objective:To investigate the regulatory effects of two traditional mineral medicines(TMMs),Gypsum Fibrosum(Shigao,GF)and Terra Flava Usta(Zaoxintu,TFU),on gut-beneficial bacteria in mice,and preliminarily explore thei...Objective:To investigate the regulatory effects of two traditional mineral medicines(TMMs),Gypsum Fibrosum(Shigao,GF)and Terra Flava Usta(Zaoxintu,TFU),on gut-beneficial bacteria in mice,and preliminarily explore their mechanisms of action.Methods:Mice were randomly divided into 3 groups(n=10 per group):the control group(standard diet),the GF group(diet supplemented with 2%GF),and the TFU group(diet supplemented with 2%TFU).After 4-week intervention,16S rRNA gene sequencing was used to analyze the changes in the gut microbiota(GM).Scanning electron microscopy,in combination with coumarin A tetramethyl rhodamine conjugate and Hoechst stainings,was used to observe the bacteria and biofilm formation.Results:Principal coordinate analysis revealed that GF and TFU significantly altered the GM composition in mice.Further analysis revealed that GF and TFU affected different types of gut bacteria,suggesting that different TMMs may selectively modulate specific bacterial populations.For certain bacteria,such as Faecalibaculum and lleibacterium,both GF and TFU exhibited growth-promoting effects,implying that they may be sensitive to TMMs and that different TMMs can increase their abundance through their respective mechanisms.Notably,Lactobacillus reuteri,a widely recognized and used probiotic,was significantly enriched in the GF group.Random forest analysis identified lleibacterium valens as a potential indicator bacterium for TMMs'impact on GM.Further mechanistic studies showed that gut bacteria formed biofilm structures on the TFU surface.Conclusions:This study provides new insights into the interaction between TMMs and GM.As safe and effective natural clays,GF and TFU hold promise as potential candidates for prebiotic development.展开更多
文摘The research on the application of X-ray diffraction in the quantitative analysis of Chinese medicines is rare. The main reason is that the technical problems related to the internal standard and the selection of quantitative peaks are not well solved, and the accuracy and precision of the results are not satisfactory. This study employed the concept of mass absorption coefficient based on the internal standard method, and the full spectrum fitting and quantitative methods were used to solve the above technical problems. The sample was blended. the internal standard substance of zinc oxide was fully ground, and tablets were prepared by positive pressure method. Under certain instrumental conditions, the PXRD pattern was obtained by scanning. The percentage of gypsum fibrosum in Xiaokening tablet was obtained by quantitative analysis of full spectrum fitting internal standard by TOPAS software. The method was investigated by methodology. At the same time, the method was compared by ion chromatography, and SPSS software was used to make a significant t test on the results of the two methods. After the investigation, the average standard recovery rate of CaSO4-2H2O was 99.06%(RSD = 3.02%);and the recovery rate for simulated samples was 96.7%. The method had good specificity. After statistical analysis, there was no significant difference between the new PXRD method and the traditional method of ion chromatography.
基金Supported by Natural Science Foundation of China(No.31770540)the Key Research Program of Jiangsu Province(No.BE2018663)。
文摘Objective:To investigate the regulatory effects of two traditional mineral medicines(TMMs),Gypsum Fibrosum(Shigao,GF)and Terra Flava Usta(Zaoxintu,TFU),on gut-beneficial bacteria in mice,and preliminarily explore their mechanisms of action.Methods:Mice were randomly divided into 3 groups(n=10 per group):the control group(standard diet),the GF group(diet supplemented with 2%GF),and the TFU group(diet supplemented with 2%TFU).After 4-week intervention,16S rRNA gene sequencing was used to analyze the changes in the gut microbiota(GM).Scanning electron microscopy,in combination with coumarin A tetramethyl rhodamine conjugate and Hoechst stainings,was used to observe the bacteria and biofilm formation.Results:Principal coordinate analysis revealed that GF and TFU significantly altered the GM composition in mice.Further analysis revealed that GF and TFU affected different types of gut bacteria,suggesting that different TMMs may selectively modulate specific bacterial populations.For certain bacteria,such as Faecalibaculum and lleibacterium,both GF and TFU exhibited growth-promoting effects,implying that they may be sensitive to TMMs and that different TMMs can increase their abundance through their respective mechanisms.Notably,Lactobacillus reuteri,a widely recognized and used probiotic,was significantly enriched in the GF group.Random forest analysis identified lleibacterium valens as a potential indicator bacterium for TMMs'impact on GM.Further mechanistic studies showed that gut bacteria formed biofilm structures on the TFU surface.Conclusions:This study provides new insights into the interaction between TMMs and GM.As safe and effective natural clays,GF and TFU hold promise as potential candidates for prebiotic development.
文摘目的：探讨麻杏石甘汤配伍对其解热作用的影响.方法：利用混料均匀设计,以麻黄（X1）、苦杏仁（X2）、炙甘草（X3）和石膏（X4）4个组分设计4因素12水平的麻杏石甘汤配比实验.将SD健康雄性大鼠随机分为15组,分别为麻杏石甘汤配比N1 ～ N12组、临床常用方组、模型组、空白组.采用尾静脉注射脂多糖（LPS）造模,造模后每0.5h记录1次体温,计算体温反应指数（TRI）;测定造模后6.0h血浆前列腺素E2（PGE2）含量;对结果进行二次多项式回归分析.结果：各配伍组与模型组比较,均能不同程度的抑制LPS诱导的大鼠发热,具有极显著性意义（P＜0.01）;经麻杏石甘汤干预后,血浆PGE2有不同程度的下降;经麻杏石甘汤干预后,TRI有不同程度的下降.回归分析的结果表明,影响TRI的因素为石膏＞麻黄＞苦杏仁与炙甘草的交互作用.麻黄与体温反应指数为负相关.结论：4因素12水平的混料均匀设计实验结果表明：对于疗热,方中石膏为君药,最佳配比剂量为麻黄0.6g,苦杏仁7.5g,甘草7.5g,石膏26.4 g.
