The study of the mechanism by which plant polyphenols regulate lipid metabolism homeostasis has become a cutting-edge direction in the field of functional foods.Our recent research has reported the inhibitory effect o...The study of the mechanism by which plant polyphenols regulate lipid metabolism homeostasis has become a cutting-edge direction in the field of functional foods.Our recent research has reported the inhibitory effect of Hyperoside(Hyp)on lipase was significantly better than that of the orlistat and other main polyphenols from mulberry leaf.The present study further explored the pathways through which it regulated lipid metabolism.Firstly,a HepG2 adipose accumulation model was established to preliminarily explore the effect of Hyp on lipid metabolism;Further,the in-vivo efficacy was validated through a high-fat diet induced hyperlipidemia C57BL/6 mice model.Finally,the molecular mechanisms were investigated by combining untargeted metabolomics,KEGG enrichment pathway analysis,and RT-qPCR key genes validation.The results showed that Hyp exhibited a significant reducing effect on TC in HepG2 adipocytes.Besides,Hyp significantly reduced serum and liver TC and TG,and reduced the serum LDL-C content by upregulating the expression of LDL-R gene in hyperlipidemic mice.Hyp was metabolized to produce characteristic metabolites such as miquelianin,quercetin,morin,3-O-methyl-quercetin,and luteolin,which activated glycerophospholipid metabolism,regulation of lipolysis in adipocytes,and primary bile acid biosynthesis pathways,and exerted lipid metabolism regulation effects through the“gut-liver axis”Fiaf/CYP7A1 signaling pathway.展开更多
目的:探讨化石利胆汤对逆行胰胆管造影取石(ERCP)术后胆总管结石患者血液以及血脂中的胆固醇7α羟化酶以及二磷酸葡萄糖醛酸基转移酶的影响。方法:选取90例行ERCP检查且证实胆总管结石并行碎石取石术患者,将其随机分为治疗组及对照组,每...目的:探讨化石利胆汤对逆行胰胆管造影取石(ERCP)术后胆总管结石患者血液以及血脂中的胆固醇7α羟化酶以及二磷酸葡萄糖醛酸基转移酶的影响。方法:选取90例行ERCP检查且证实胆总管结石并行碎石取石术患者,将其随机分为治疗组及对照组,每组45例,治疗组给予常规治疗结合化石利胆汤,对照组予常规治疗,分别比较治疗前以及治疗后第7天、1个月患者血CYP7A1、B-UGT m RNA以及蛋白的表达情况。结果:治疗组服药后第7天、1个月的CYP7A1、B-UGT m RNA表达水平均明显高于治疗前,且与对照组同时期比较明显升高,治疗组服药治疗后第7天的CYP7A1、B-UGT蛋白表达水平明显较治疗前升高,且与对照组同时期比较明显升高,组间比较差异均有统计学意义(P<0.05)。结论:化石利胆汤可上调胆总管结石ERCP术后患者CYP7A1、B-UGT m RNA及其蛋白的表达。展开更多
基金funding from the Science&Technology Pro-jects of Guangdong Province[2024A1515011122]the Collaborative Innovation Center of GDAAS[XTXM202205,XT202502]+2 种基金the contribution of Innovative Research Team Construction Project for Modern Agricultural Industry Common Key Technologies of Guangdong Province[2024CXTD16]the High-level Guangdong Province Agricultural Science and Technology Demonstration City Construction Fund Project in 2023[2320060002429]the China Agriculture Research System of MOF and MARA[CARS-18-ZJ0503].
文摘The study of the mechanism by which plant polyphenols regulate lipid metabolism homeostasis has become a cutting-edge direction in the field of functional foods.Our recent research has reported the inhibitory effect of Hyperoside(Hyp)on lipase was significantly better than that of the orlistat and other main polyphenols from mulberry leaf.The present study further explored the pathways through which it regulated lipid metabolism.Firstly,a HepG2 adipose accumulation model was established to preliminarily explore the effect of Hyp on lipid metabolism;Further,the in-vivo efficacy was validated through a high-fat diet induced hyperlipidemia C57BL/6 mice model.Finally,the molecular mechanisms were investigated by combining untargeted metabolomics,KEGG enrichment pathway analysis,and RT-qPCR key genes validation.The results showed that Hyp exhibited a significant reducing effect on TC in HepG2 adipocytes.Besides,Hyp significantly reduced serum and liver TC and TG,and reduced the serum LDL-C content by upregulating the expression of LDL-R gene in hyperlipidemic mice.Hyp was metabolized to produce characteristic metabolites such as miquelianin,quercetin,morin,3-O-methyl-quercetin,and luteolin,which activated glycerophospholipid metabolism,regulation of lipolysis in adipocytes,and primary bile acid biosynthesis pathways,and exerted lipid metabolism regulation effects through the“gut-liver axis”Fiaf/CYP7A1 signaling pathway.
文摘目的:探讨化石利胆汤对逆行胰胆管造影取石(ERCP)术后胆总管结石患者血液以及血脂中的胆固醇7α羟化酶以及二磷酸葡萄糖醛酸基转移酶的影响。方法:选取90例行ERCP检查且证实胆总管结石并行碎石取石术患者,将其随机分为治疗组及对照组,每组45例,治疗组给予常规治疗结合化石利胆汤,对照组予常规治疗,分别比较治疗前以及治疗后第7天、1个月患者血CYP7A1、B-UGT m RNA以及蛋白的表达情况。结果:治疗组服药后第7天、1个月的CYP7A1、B-UGT m RNA表达水平均明显高于治疗前,且与对照组同时期比较明显升高,治疗组服药治疗后第7天的CYP7A1、B-UGT蛋白表达水平明显较治疗前升高,且与对照组同时期比较明显升高,组间比较差异均有统计学意义(P<0.05)。结论:化石利胆汤可上调胆总管结石ERCP术后患者CYP7A1、B-UGT m RNA及其蛋白的表达。
