Fusarium graminearum is a fungal plant pathogen which causes Fusarium head blight(FHB), a devastating diseaseon cereal crops. Here we report that FgPMA1 could be a new target to control FHB by the application of doubl...Fusarium graminearum is a fungal plant pathogen which causes Fusarium head blight(FHB), a devastating diseaseon cereal crops. Here we report that FgPMA1 could be a new target to control FHB by the application of double-stranded RNA(dsRNA) of FgPMA1. FgPMA1 was divided into 6 segments to generated RNA interference(RNAi)constructs(FgPMA1RNAi-1,-2,-3,-4,-5, and-6), and these constructs were transformed in F. graminearum strainPH-1. The expression of FgPMA1 reduced by 18.48, 33.48 and 56.93% in FgPMA1RNAi-1, FgPMA1RNAi-2 and FgPMA1RNAi-5, respectively. FgPMA1RNAi-1,-2, and-5 mutants inhibited fungal development, including mycelium growth, mycelial morphology, asexual and sexual development, and toxin production. The length of lesions on wheat leaves, wheat coleoptiles and wheat ears were shorter after infection with FgPMA1RNAi-1,-2, and-5 mutants thanwild type PH-1. These results showed that three segments(FgPMA1RNAi-1,-2, and-5) exhibited effective silencing effects. After treatment with 25 ng μL^(–1)dsRNA of these segments in vitro, the growth rate of mycelium growth was significant decreased, mycelium became deformed with bulbous structure at the tip, and the mycelium lost the ability to produce conidia in F. graminearum strain PH-1, Fusarium asiacitum strain 2021 and phenamacril-resistant strainYP-1. After application of FgPMA1RNAi-1-dsRNA and FgPMA1RNAi-2-dsRNA to wheat ears, pathogenicity reduced 34.21–35.40%.展开更多
基金sponsored by the National Natural Science Foundation of China (32272585)the National Key R&D Program of China (2022YFD1400900)the Fundamental Research Funds for the Central Universities, China (KYCXJC2023003)。
文摘Fusarium graminearum is a fungal plant pathogen which causes Fusarium head blight(FHB), a devastating diseaseon cereal crops. Here we report that FgPMA1 could be a new target to control FHB by the application of double-stranded RNA(dsRNA) of FgPMA1. FgPMA1 was divided into 6 segments to generated RNA interference(RNAi)constructs(FgPMA1RNAi-1,-2,-3,-4,-5, and-6), and these constructs were transformed in F. graminearum strainPH-1. The expression of FgPMA1 reduced by 18.48, 33.48 and 56.93% in FgPMA1RNAi-1, FgPMA1RNAi-2 and FgPMA1RNAi-5, respectively. FgPMA1RNAi-1,-2, and-5 mutants inhibited fungal development, including mycelium growth, mycelial morphology, asexual and sexual development, and toxin production. The length of lesions on wheat leaves, wheat coleoptiles and wheat ears were shorter after infection with FgPMA1RNAi-1,-2, and-5 mutants thanwild type PH-1. These results showed that three segments(FgPMA1RNAi-1,-2, and-5) exhibited effective silencing effects. After treatment with 25 ng μL^(–1)dsRNA of these segments in vitro, the growth rate of mycelium growth was significant decreased, mycelium became deformed with bulbous structure at the tip, and the mycelium lost the ability to produce conidia in F. graminearum strain PH-1, Fusarium asiacitum strain 2021 and phenamacril-resistant strainYP-1. After application of FgPMA1RNAi-1-dsRNA and FgPMA1RNAi-2-dsRNA to wheat ears, pathogenicity reduced 34.21–35.40%.
