Objective Establishing a highly sensitive real-time fluorescence quantitative PCR (qPCR) method for universal testing of epidemic African swine fever virus (ASFV) strains. Methods The ASFV p72 gene was targeted to des...Objective Establishing a highly sensitive real-time fluorescence quantitative PCR (qPCR) method for universal testing of epidemic African swine fever virus (ASFV) strains. Methods The ASFV p72 gene was targeted to design primer probes covering 24 p72 genotypes. The optimal amount of dimethylsulphoxide (DMSO) for qPCR amplification was determined, Various sensitivity and limit of detection (LOD) tests were performed, and clinical samples from China and imported goods were tested. Results The optimal primer-probe combination could specifically detect ASFV, 1.5% DMSO was optimal for qPCR, and LOD reached 3.2 copies/μL with good reproducibility (n = 20, p = 0.369). The method was employed to test 142 clinically suspected samples, of which 30 pig blood and 37 pig tissue samples were ASFV-positive. Moreover, the positive testing rate for ASFV was higher than for the standard qPCR method recommended by the Office International Des Epizooties (OIE), and for the commercially available kit. Thus, our method is superior for testing weakly positive samples with low virus titre, and epidemic strains present in imported goods. Conclusion Our method could be employed for universal testing of epidemic ASFV strains worldwide, ensuring wider coverage of hosts and ASFV strains/endemic strains, reducing false<span style="font-family:;" "=""> </span><span style="font-family:Verdana;">negatives, and benefitting early diagnosis.</span>展开更多
目的本研究旨在分析腰椎手术引流管拔除后患者发热的相关危险因素。方法回顾性分析自2018年1月—2024年1月于阜外华中心血管病医院行腰椎后路手术治疗的216例腰椎疾病患者,收集患者年龄、性别、体重指数(body mass index,BMI)、手术时...目的本研究旨在分析腰椎手术引流管拔除后患者发热的相关危险因素。方法回顾性分析自2018年1月—2024年1月于阜外华中心血管病医院行腰椎后路手术治疗的216例腰椎疾病患者,收集患者年龄、性别、体重指数(body mass index,BMI)、手术时间、出血量、融合节段、减压节段、术中应用明胶海绵数量、引流管留置时间、吸烟史、是否合并糖尿病等临床资料并进行统计学分析。结果216例患者,发热组84例,占比38.89%,非发热组132例,占比61.11%,发热组和非发热组患者术中出血量、引流管留置时间、手术时间比较,差异有统计学意义(P<0.05);经多因素logistic回归分析结果显示,术中出血量多和手术时间长是腰椎手术拔管后发热的独立危险因素(OR>1,P<0.05),术后1周内引流管留置时间长是腰椎手术拔管后发热的保护性因素(OR<1,P<0.05)。结论术中出血量多、手术时间长是腰椎手术拔管后发热的危险因素。展开更多
文摘Objective Establishing a highly sensitive real-time fluorescence quantitative PCR (qPCR) method for universal testing of epidemic African swine fever virus (ASFV) strains. Methods The ASFV p72 gene was targeted to design primer probes covering 24 p72 genotypes. The optimal amount of dimethylsulphoxide (DMSO) for qPCR amplification was determined, Various sensitivity and limit of detection (LOD) tests were performed, and clinical samples from China and imported goods were tested. Results The optimal primer-probe combination could specifically detect ASFV, 1.5% DMSO was optimal for qPCR, and LOD reached 3.2 copies/μL with good reproducibility (n = 20, p = 0.369). The method was employed to test 142 clinically suspected samples, of which 30 pig blood and 37 pig tissue samples were ASFV-positive. Moreover, the positive testing rate for ASFV was higher than for the standard qPCR method recommended by the Office International Des Epizooties (OIE), and for the commercially available kit. Thus, our method is superior for testing weakly positive samples with low virus titre, and epidemic strains present in imported goods. Conclusion Our method could be employed for universal testing of epidemic ASFV strains worldwide, ensuring wider coverage of hosts and ASFV strains/endemic strains, reducing false<span style="font-family:;" "=""> </span><span style="font-family:Verdana;">negatives, and benefitting early diagnosis.</span>
文摘目的本研究旨在分析腰椎手术引流管拔除后患者发热的相关危险因素。方法回顾性分析自2018年1月—2024年1月于阜外华中心血管病医院行腰椎后路手术治疗的216例腰椎疾病患者,收集患者年龄、性别、体重指数(body mass index,BMI)、手术时间、出血量、融合节段、减压节段、术中应用明胶海绵数量、引流管留置时间、吸烟史、是否合并糖尿病等临床资料并进行统计学分析。结果216例患者,发热组84例,占比38.89%,非发热组132例,占比61.11%,发热组和非发热组患者术中出血量、引流管留置时间、手术时间比较,差异有统计学意义(P<0.05);经多因素logistic回归分析结果显示,术中出血量多和手术时间长是腰椎手术拔管后发热的独立危险因素(OR>1,P<0.05),术后1周内引流管留置时间长是腰椎手术拔管后发热的保护性因素(OR<1,P<0.05)。结论术中出血量多、手术时间长是腰椎手术拔管后发热的危险因素。
