[Objective] The aim was to research relationship between SPAD decline index after full-heading stage （SDIFHS） and productivity of rice. fertilized with nitro- gen in order to provide theoretical and practical refere...[Objective] The aim was to research relationship between SPAD decline index after full-heading stage （SDIFHS） and productivity of rice. fertilized with nitro- gen in order to provide theoretical and practical references for selection and breed- ing of rice varieties. [Method] From 2008 to 2010, 18 mid-season hybrid rice vari- eties were researched every year to explore relationship＇ between SDIFHS and pro- ductivity of rice fertilized with nitrogen. [Result] The productivity of rice fertilized with nitrogen was of extremely significant positive corretation with SDIFHS, because the higher SPAD decline index is, the higher LAI decline index and the transformation ratio of dry matter to spikes in overground plant would be. [Conclusion] The re- search established a new method to predict productivity of rice fertilized with nitro- gen based on SPAD decline index.展开更多
In order to investigate the effect of temperature on hatching result of fertilized peacock eggs, they were hatched under conditions of varying temperature and constant temperature respectively. The result shows that t...In order to investigate the effect of temperature on hatching result of fertilized peacock eggs, they were hatched under conditions of varying temperature and constant temperature respectively. The result shows that the hatching rate in the varying temperature treatment group （Group Ⅰ） is higher than that in the constant temperature treatment group （Group Ⅱ）. It suggests that the varying temperature treatment （high temperature in the early period, medium temperature in the middle period, slightly lower temperature in the late period, and slightly higher temperature in the hulling period） is in line with the optimum temperature variation in the hatching of fertilized peacock eggs, contributing the hatching of fertilized peacock eggs.展开更多
In order to screen the effective preventive drugs for saprolegniasis occurred during hatching of the fertilized eggs of golden trout, effects of two kinds of self-made Chinese herbal compounds（AB） and two kinds of c...In order to screen the effective preventive drugs for saprolegniasis occurred during hatching of the fertilized eggs of golden trout, effects of two kinds of self-made Chinese herbal compounds（AB） and two kinds of chemical bactericides（peroxyacetic acid andhydrogen peroxide, CD） with three disinfection concentrations were compared in this study. The fertilized eggs of golden trout were randomly divided into 13 groups, each group with two parallels of 1 000 eggs. Drugs at high,medium and low concentration were respectively used to immerse the fertilized eggs35 min for disinfection once a day. The numbers of live and dead eggs were recorded each day before disinfection and the numbers of survival eyed eggs were counted in each experimental group after finishing the whole test. The results indicated that all of four kinds of drugs used here had a certain effect on prevention and control of saprolegniasis during hatching of fertilized eggs of golden trout, and also showed a correlation between the concentrations and effects. After the selection of several drugs, we found that group B-medium, C-medium and D-high exhibited the most remarkable effects on control of saprolegniasis. However, careful consideration should be given to chemical drugs residues and their potential hazard to the eyed eggs. Therefore, Chinese herbal compound B was suggested to be used for prevention and control of saprolegniasis to achieve the healthy aquaculture. And the concentration and operation time of Chinese herbal compound B should be strictly controlled when used in disinfection of fertilized eggs.展开更多
MicroRNAs（miRNAs） are a class of highly conserved small non-coding RNA molecules that play a pivotal role in several cellular functions.In this study,miRNA and messenger RNA（mRNA） profiles were examined by Illumin...MicroRNAs（miRNAs） are a class of highly conserved small non-coding RNA molecules that play a pivotal role in several cellular functions.In this study,miRNA and messenger RNA（mRNA） profiles were examined by Illumina microarray in mouse embryonic stem cells（ESCs） derived from parthenogenetic,androgenetic,and fertilized blastocysts.The global analysis of miRNA-mRNA target pairs provided insight into the role of miRNAs in gene expression.Results showed that a total of 125 miRNAs and 2394 mRNAs were differentially expressed between androgenetic ESCs（aESCs） and fertilized ESCs（fESCs）,a total of 42 miRNAs and 87 mRNAs were differentially expressed between parthenogenetic ESCs（pESCs） and fESCs,and a total of 99 miRNAs and 1788 mRNAs were differentially expressed between aESCs and pESCs.In addition,a total of 575,5 and 376 miRNA-mRNA target pairs were observed in aESCs vs.fESCs,pESCs vs.fESCs,and aESCs vs.pESCs,respectively.Furthermore,15 known imprinted genes and 16 putative uniparentally expressed miRNAs with high expression levels were confirmed by both microarray and real-time RT-PCR.Finally,transfection of miRNA inhibitors was performed to validate the regulatory relationship between putative maternally expressed miRNAs and target mRNAs. Inhibition of miR-880 increased the expression of Peg3,Dyrklb,and Prrg2 mRNA,inhibition of miR-363 increased the expression of Nfat5 and Soatl mRNA,and inhibition of miR-883b-5p increased Nfat5,Tacstd2,and Ppapdc1 mRNA.These results warrant a functional study to fully understand the underlying regulation of genomic imprinting in early embryo development.展开更多
This study aims to evaluate the production cost of Heterobranchus longifilis larvae reared in fertilized fish ponds. Experiment was carried out in two fish ponds. Each fish pond was filled with 200 liters of water and...This study aims to evaluate the production cost of Heterobranchus longifilis larvae reared in fertilized fish ponds. Experiment was carried out in two fish ponds. Each fish pond was filled with 200 liters of water and fertilized with dry poultry droppings (dose: 0.6 g.L-1) and zooplankton was inoculated with 83±13 individuals/L. Six days after inoculation of zooplankton, larvae of H. longifilis of 2 days after post-hatching were directly introduced into ponds with 300 individuals/m3. These larvae used exclusively zooplankton during the first 7 days. From the 8th day, larvae were fed with Coppens feed until 30 days. At the end, the survival rates were ranged between 58.33% and 56.67%. Final mean weights were brought up to all the ponds and were notsignificantly different (p > 0.05). The production costs ranged between 2.55 FCFA and 2.60 FCFA per fingerling with ratio production cost/weight gain around 0.80 FCFA/g in each pond. Then, this production system of fingerlings in fertilized ponds is very efficient and could be popularized in the rural fish farms.展开更多
Biologically active soil organic carbon （BASOC） is an important fraction of soil organic carbon （SOC）, but our understanding of the correlation between BASOC and soil aggregate stability is limited. At an ecologic...Biologically active soil organic carbon （BASOC） is an important fraction of soil organic carbon （SOC）, but our understanding of the correlation between BASOC and soil aggregate stability is limited. At an ecological experimental station （28° 04＇-28° 37＇ N, 116° 41＇-117° 09＇ E） in Yujiang County, Jiangxi Province, China, we analyzed the dynamic relationship between soil aggregate stability and BASOC content over time in the red soil （Udic Ferrosols） fertilized with a nitrogen-phosphorus-potassium chemical fertilizer （NPK） without manure or with NPK plus livestock manure or green manure. The dynamics of BASOC was evaluated using CO2 efflux, and soil aggregates were separated according to size using a wet-sieving technique. The soils fertilized with NPK plus livestock manure had a significantly higher content of BASOC and an improved aggregate stability compared to the soils fertilized with NPK plus green manure or NPK alone. The BASOC contents in all fertilized soils decreased over time. The contents of large aggregates （800-2 000 μm） dramatically decreased over the first 7 d of incubation, but the contents of small aggregates （〈 800 μm） either remained the same or increased, depending on the incubation time and specific aggregate sizes. The aggregate stability did not differ significantly at the beginning and end of incubation, but the lowest stability in all fertilized soils occurred in the middle of the incubation, which implied that the soils had a strong resilience for aggregate stability. The change in BASOC content was only correlated with aggregate stability during the first 27 d of incubation.展开更多
The production of nitrous oxide (N2O) is a widespread trait in fungi and is of interest because denitrifying fungi lack the N2O reductase gene (nosZ) that regulates N2O reduction to nitrogen gas (N2). The adaptive abi...The production of nitrous oxide (N2O) is a widespread trait in fungi and is of interest because denitrifying fungi lack the N2O reductase gene (nosZ) that regulates N2O reduction to nitrogen gas (N2). The adaptive ability of soil fungi is better than that of bacteria in acidic soils. We investigated the N2O reduction potential, described by the N2O product ratio (Nn2O), N2O/(N2O+N2), in soils of different types of fields under crop cultivation with different fertilizer inputs and a bare fallow field with no fertilization as a control. The fungi-to-bacteria abundance ratio (Rf/b) was negatively correlated (P < 0.01) with the natural pH of the soil;however, the high value of Rp/b measured in vineyards was due to the large inputs of manure. When the denitrification potential was measured at natural pH values of soils, Nn2O was negatively correlated (P < 0.01) with soil pH. When the denitrification potential was measured after short-term modifications of soil pH, however, no significant correlation was found between Nn2O and the modified pH. Based on stepwise multiple regression analysis, soil pH and residual nitrate (NO3) were the key factors regulating N2O reduction in soils at natural pH values (R^2 = 0.8& P < 0.001), whereas the key factor was the soil residual NO, alone (R^2 = 0.83, P < 0.001) when the soil pH was modified. When the effect of the soil chemical properties was weakened, a high Rf/b value had the potential (P < 0.01) to affect N2O reduction;however, the role of fungi was offset by the presence of denitrifying bacteria. These results provide evidence that compared to the indirect effects of Rf/b、the direct effects of the soil chemical properties have a greater effect on N2O reduction in fertilized soils.展开更多
Fertilizers are widely applied in agricultural practice to improve crop yield and quality.However, they can also alter the behavior of soil pollutants.A field experiment was conducted on a chernozem in Heilongjiang Pr...Fertilizers are widely applied in agricultural practice to improve crop yield and quality.However, they can also alter the behavior of soil pollutants.A field experiment was conducted on a chernozem in Heilongjiang Province, China.Various proportions of inorganic nitrogen(N), phosphorus(P), and organic(manure) fertilizers were applied.Soil samples were collected four times over 2 years, and the total concentrations and speciation of chromium(Cr) and cadmium(Cd) were analyzed.Inorganic fertilizer addition had little effect on the total concentration of Cr and Cd, while organic fertilizer addition reduced the total concentrations of both Cr and Cd.Inorganic fertilizers increased the concentration of Cr in the exchangeable form, but decreased that of the residual form(P < 0.05).Addition of inorganic or organic fertilizer alone decreased concentrations of exchangeable Cd(P < 0.05), but the combination of inorganic and organic fertilizers had the opposite effect.High concentration of total Cd exceeding the upper limits of the Environmental Quality Standard for Soils proposed by the Ministry of Environmental Protection of China could result in an environmental risk, and fertilizer application could lower that risk.Results of risk assessment code calculated as the percentage of the sum of metal concentrations in the exchangeable and carbonate-associated form in total metal concentration showed that Cr and Cd risks were lowered when organic fertilizer was applied alone or in combination with inorganic fertilizers.Our study highlighted that organic fertilizer should be applied alone or in combination with inorganic fertilizers to lower the environmental risks of Cr and Cd pollution in the chernozem.展开更多
This article presents a case of conservative managements of an in vitro-fertilized twin pregnancy with early loss of the first fetus. A 37-year-old woman in the 21st week of a twin pregnancy delivered her one of the f...This article presents a case of conservative managements of an in vitro-fertilized twin pregnancy with early loss of the first fetus. A 37-year-old woman in the 21st week of a twin pregnancy delivered her one of the fetuses with unfortunate outcome after the premature rupture of membranes. The umbilical cord of the first fetus was ligated at the cervical level and the second surviving fetus was left in utero. In order to save the surviving fetus, conservative managements including combination of steroids, tocolytics, antibiotics and cervical cerclage were performed. The second fetus was delivered vaginally in the 27th week of pregnancy, 4 h after premature rupture of membranes of the remaining amniotic sac, 41 d after the first fetus was born. The child and the mother have been followed up for 4 years and they are healthy. Conservative managements including steroids, tocolytics, antibiotics and cervical cerclage in a closely monitored environment would be given following the delivery of the first fetus. Delayed-interval pregnancy appears to be safe for the mother and the remaining fetuses.展开更多
Lysophosphatidic acid(LPA)is a small molecule glycerophospholipid,which regulates multiple downstream signalling pathways through G-protein-coupled receptors to achieve numerous functions on oocyte maturation and embr...Lysophosphatidic acid(LPA)is a small molecule glycerophospholipid,which regulates multiple downstream signalling pathways through G-protein-coupled receptors to achieve numerous functions on oocyte maturation and embryo development.In this study,sheep in vitro fertilized embryos were applied to investigate the effects of LPA on early embryos development and embryonic stem cell establishment.At first,the maturation medium containing estrus female sheep serum and synthetic oviduct fluid(SOF)were optimized for sheep IVF,and then the effects of LPA were investigated.From 0.1 to 10μmol L^(–1),LPA had no significant effect on the cleavage rate(P>0.05),but the maturation rate and blastocyst rate increased dependently with LPA concentration(P<0.05),and the blastocyst morphology was normal.When the LPA concentration was 15μmol L^(–1),the maturation rate,cleavage rate and blastocyst rate decreased significantly(P<0.05),and the blastocyst exhibited abnormal morphology and could not develop into highquality blastocyst.Besides,the exogenous LPA increases the expression of LPAR2,LPAR4,TE-related gene CDX-2and pluripotency-related gene OCT-4 in sheep early IVF embryos with the raise of LPA concentration from 0.1 to 10μmol L^(–1).The expression of LPAR2,LPAR4,CDX-2 and OCT-4 from the LPA-0.1μmol L^(–1)to LPA-10μmol L^(–1)groups in early embryos were extremely significant(P<0.05),while the expression of these genes significantly decreased in 15μmol L^(–1)LPA-treated embryos compared with LPA-10μmol L^(–1)group(P<0.05).The inner cell mass in 15μmol L^(–1)LPA-treated embryos was also disturbed,and the blastocysts formation was abnormal.Secondly,the sheep IVF blastocysts were applied to establish embryonic stem cells.The results showed that LPA made the blastocyst inoculated cells grow towards TSC-like cells.They enhanced the fluorescence intensity and mRNA abundance of OCT-4 and CDX-2 as the concentration increased from 0 to 10μmol L^(–1),while 15μmol L^(–1)LPA decreased OCT-4 and CDX-2 expression in the derived cells.The expression of CDX-2 and OCT-4 in the blastocyst inoculated cells of LPA-1μmol L^(–1)group and LPA-10μmol L^(–1)group extremely significantly increased(P<0.05),but there was significant decrease in LPA-15μmol L^(–1)group compared with LPA-10μmol L^(–1)group(P<0.05).Meanwhile,the protein expression of LPAR2 and LPAR4 remarkably increased after treatment of LPA at 10μmol L^(–1)concentration.This study references the IVF embryo production and embryonic stem cell research of domestic animals.展开更多
We compared chemical and biological properties of soils in organically and conventionally fertilized apple orchards in Nagano Prefecture (one of the major apple producing regions in Japan). Five apple orchards with di...We compared chemical and biological properties of soils in organically and conventionally fertilized apple orchards in Nagano Prefecture (one of the major apple producing regions in Japan). Five apple orchards with different fertilizer management systems were used for this study. The total carbon and total nitrogen contents were higher in the organically fertilized orchard, while the total phosphorus and total potassium were at similar levels in both organically and conventionally fertilized orchards. The bacterial biomass did not differ between the two orchards, but the N circulation activity was clearly higher in the organically fertilized orchard from April to December. Total carbon from 50,000 to 60,000 mg/kg, total nitrogen at about 3000 to 4000 mg/kg, and a C/N ratio of 15 - 20 were suggested to be suitable conditions for a high level of apple production under an organic fertilizer management system.展开更多
To study the sensitivity of inter-subspecific hybrid rice to climatic conditions, the spikelet fertilized rate （SFR） of four types of rice including indica-japonica hybrid, intermediate hybrid, indica and japonica w...To study the sensitivity of inter-subspecific hybrid rice to climatic conditions, the spikelet fertilized rate （SFR） of four types of rice including indica-japonica hybrid, intermediate hybrid, indica and japonica were analyzed during 2000-2004. The inter-subspecific hybrids showed lower SFR, and much higher fluctuation under various climatic conditions than indica and japonica rice, showing the inter-subspecific hybrids were sensitive to ecological conditions. Among 12 climatic factors, the key factor affecting rice SFR was temperature, with the most significant factor being the average temperature of the seven days around panicle flowering （T7）. A regressive equation of SFR-temperature by T7, and a comprehensive synthetic model by four important temperature indices were put forward. The optimum temperature for inter-subspecific hybrids was estimated to be 26.1-26.6℃, and lower limit of safe temperature to be 22.5-23.3℃ for panicle flowering, showing higher by averagely 0.5℃ and 1.7℃, respectively, to be compared with indica and japonica rice. This suggested that inter-subspecific hybrids require proper climatic conditions. During panicle flowering, the suitable daily average temperature was 23.3-29.0℃, with the fittest one at 26.1-26.6℃. For an application example, optimum heading season for inter-subspecific hybrids in key rice growing areas in China was as same as common pure lines, while inferior limit for safe date of heading was about a ten-day period earlier than those of common pure lines.展开更多
Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of preimplantation embryos. ESCs exhibit true pluripotency, i.e., the ability to differentiate into cells of all three germ layers in the developi...Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of preimplantation embryos. ESCs exhibit true pluripotency, i.e., the ability to differentiate into cells of all three germ layers in the developing embryo. We used 2-DE MALDI-TOF/TOF to identify differentially expressed proteins among three types of mouse embryonic stem cells (ESCs) derived from ferti-lized, parthenogenetic, and androgenetic (fESC, pESC and aESC, respectively) blastocysts. We detected more than 800 proteins on silver- stained gels of whole protein extracts from each type of ESC. Of these, 52 differentially expressed proteins were identified by the MALDI–TOF/TOF analyzer, including 32 (fESCs vs. pESCs), 28 (fESCs vs. aESCs) and 17 (pESCs vs. aESCs) prominent proteins with significantly higher or lower expression relative to the comparison cells. Among the 32 proteins from fESCs, 12 were significantly increased in expression and 20 were reduced in expression in fESCs com-pared with pESCs. Similarly, 10 of 28 and 8 of 17 proteins were more highly expressed in fESCs and pESCs compared with aESCs, respectively. In contrast, 18 of 28 and 9 of 17 proteins were reduced in expression in fESCs and pESCs compared with aESCs, respectively. Of the eight protein candidates in fESCs, four were in-creased and four were decreased in expression relative to both pESCs and aESCs in the 2-DE analysis. Differential expression of these pro-teins were confirmed by mRNA expression analysis using real time RT-PCR. For three pro-teins, ANXA5, CLIC1 and SRM, Western blot analysis corroborated the expression patterns indicated by the 2-DE results. ANXA5 and CLIC1 were increased in expression and SRM was de-creased in expression in fESCs compared with both pESCs and aESCs. The differentially ex-pressed proteins identified in the present study warrant further investigation towards the goal of their potential application in ESC therapy.展开更多
Objective: To determine the effect of preparation to play a maternal role in mothers fertilized through in vitro fertilization on maternal anxiety. Methods: A total of 60 mothers undergoing in vitro fertilization were...Objective: To determine the effect of preparation to play a maternal role in mothers fertilized through in vitro fertilization on maternal anxiety. Methods: A total of 60 mothers undergoing in vitro fertilization were assigned into intervention group and control group. Pregnancy concerns and stress questionnaires were research tools. Questionnaires were completed in both groups before organizing preparation program. Then, the preparation program was held for four sessions for the mothers in the intervention group, and the subjects in both groups were immediately investigated again after completion of preparation program and one month later. Data analyses about pregnancy stress and concerns of mothers and its dimensions were performed by repeated measure (analysis of variance), Mann-Whitney, Friedman and Wilcoxon tests. Analysis of demographic variables was performed by using independent t-test and Chi-square test in SPSS 21. Results: The score of pregnancy stress and concerns before the intervention was not significantly different between the two groups. There was a significant difference in the intervention group in the score of pregnancy stress and concerns before and after the intervention (P<0.001), before and one month after the intervention (P<0.001), immediately after and one month after the intervention (P<0.001) which was not significant in the control group. Conclusions: Maternal preparation program can be effective in reducing maternal stress and concerns.展开更多
In this experiment,the sperms were obtained from(C57×CBA) F1 male mice and the eggs from NIHstrain female mice.Using the fertilization techni-que in Vitro,the slides of chromosome of mouse eggsat the first cleava...In this experiment,the sperms were obtained from(C57×CBA) F1 male mice and the eggs from NIHstrain female mice.Using the fertilization techni-que in Vitro,the slides of chromosome of mouse eggsat the first cleavage metaphase were prepared.展开更多
The objective of this study was to examine the effects of addition of inoculant to high-nitrogen (N) fertilized timothy on fermentation, nutritive value, and feed intake of silage. The silage of timothy cultivated wit...The objective of this study was to examine the effects of addition of inoculant to high-nitrogen (N) fertilized timothy on fermentation, nutritive value, and feed intake of silage. The silage of timothy cultivated with a N fertilizer rate at two levels (high level (H), standard level (S)) was prepared with (SI, HI) or without (SC, HC) an inoculants. The CP content of H increased by 38 g·kg-1 DM compared with that of S, and the WSC and ADF contents decreased compared with those of S. Regarding the fermentation of silage, the pH and NH3-N ratio was significantly lower in the silage with inoculant (SI and HI), showing improvement of the fermentation compared with those of the silage without the addition (SC and HC). The CP content was significantly higher in HC and HI than in SC and SI, and the NDF content was the lowest in HI among the four treatments. The CP digestibility of HC and HI was significantly higher than those of SC and SI. The EE digestibility of SI was significantly higher than that of SC, and that of HI was significantly higher than that of HC. The DCP contents were significantly higher in HC and HI than in SC and SI. The TDN content was the lowest in SC and highest in HI. The DCP intakes of HC and HI were significantly higher than those of SC and SI. When the N fertilizer rate was increased, the DCP content and DCP intake of the silage increased, and the addition of inoculant improved the fermentation and increased the TDN content.展开更多
Apple/Malus x domestica cv. ＇Topaz＇/M26 and sour cherry cv. ＇Debreceni Botermo＇/Prunus mahaleb L. maidens were fertilized with different organic fertilizers and soil amendments in an organic nursery. A mycorrhizal...Apple/Malus x domestica cv. ＇Topaz＇/M26 and sour cherry cv. ＇Debreceni Botermo＇/Prunus mahaleb L. maidens were fertilized with different organic fertilizers and soil amendments in an organic nursery. A mycorrhizal inoculant, Micosat, was added to the non-fertilized control and to combinations fertilized with various bioproducts. Under assessment was the effect of nutrient delivery on the growth and development of maiden fruit trees focussing on tree height, thickness of the tree trunk, the number and the length of lateral shoots in each treatment combination. It was found that the introduction of mycorrhizal fungi into the soil without fertilizer application significantly improved the growth and development of apple and sour cherry maidens. Mineral fertilizer application （NPK） combined with mycorrhizal inoculation had a negligible effect on the above features. In the presence of bioproducts such as granular manure, Humus Active ＋ Aktywit PM, BF Amin, Vinassa and Florovit Eko, the mycorrhizal fungi were more biologically active than in the absence of these products.展开更多
Coral reef restoration approaches have often utilized adult colonies as sources for fragments (i.e. clones) to be transplanted. Although restoration through this method is fast and cheap, it has been pointed out that ...Coral reef restoration approaches have often utilized adult colonies as sources for fragments (i.e. clones) to be transplanted. Although restoration through this method is fast and cheap, it has been pointed out that it may reduce genetic diversity of the restored population. Low genetic diversity is a concern for reef restoration when seed fragments are raised asexually from only a few donor colonies. This can lead to lower fertilization rates among seed fragments, and reducing the longterm benefits of reef restoration in particular areas. Additionally, low genetic diversity can compound the effects of increased ocean temperature and other environmental stressors, further jeopardizing the health of a reef. An alternative approach through sexually propagated coral cultures and out-plantings can alleviate this problem. Sexually produced offsprings are more genetically diverse. They can be produced in far greater numbers than coral fragments and do not imply destructive methods. Ongoing research at the Akajima Marine Science Laboratory in Okinawa, Japan has helped to improve the production and maintenance of sexually propagated larval cultures. Our results show that crosses between gametes from 6 or more colonies will provide the highest fertilization rate (>95%). Based on the results, we suggest the use of 6 or more donor colonies for practical gamete fertilization in sexually derived coral culture.展开更多
Rationale:This case report describes a couple with recurrent fertilization failure despite undergoing multiple cycles of intracytoplasmic sperm injection(ICSI).The principal clinical concern was suspected oocyte activ...Rationale:This case report describes a couple with recurrent fertilization failure despite undergoing multiple cycles of intracytoplasmic sperm injection(ICSI).The principal clinical concern was suspected oocyte activation deficiency(OAD),in which fertilization is impeded due to the oocyte’s inability to initiate embryogenesis,commonly attributed to inadequate intracellular calcium(Ca^(2+))release following sperm injection.Patient concerns:The couple repeatedly experienced complete or near-complete fertilization failure in previous ICSI cycles,raising suspicion of an underlying oocyte activation defect.Diagnosis:Based on the repeated absence of fertilization post-ICSI and clinical history,a diagnosis of suspected OAD leading to recurrent ICSI fertilization failure was considered.Interventions:Artificial oocyte activation(AOA)using the calcium ionophore A23187 was performed.After ICSI,unfertilized oocytes were exposed to the ionophore to induce Ca^(2+)influx,simulating physiological calcium oscillations essential for oocyte activation.The efficacy of intervention was evaluated through subsequent embryonic development,morphological grading,and chromosomal integrity.Outcomes:Following AOA treatment,successful oocyte activation occurred,resulting in the formation of high-grade embryos with normal developmental progression.Chromosomal analysis revealed no detectable abnormalities,indicating genomic stability.Lessons:Calcium ionophore–mediated AOA may serve as an effective adjunct in cases of recurrent ICSI failure attributed to OAD.This case highlights the importance of individualized therapeutic strategies in assisted reproduction;however,further research is needed to refine protocols,validate broader clinical efficacy,and assess long-term safety,including potential epigenetic risks.展开更多
One unresolved challenge in the egg industry is how to efficiently and non-invasively detect unfertilized eggs prior to incubation.This detection ability would not only significantly improve hatching rates and reduce ...One unresolved challenge in the egg industry is how to efficiently and non-invasively detect unfertilized eggs prior to incubation.This detection ability would not only significantly improve hatching rates and reduce costs but also conserve incubator space and prevent poor-quality embryos from contributing to the spread of infections.This study demonstrates a procedure for distinguishing between fertilized and unfertilized eggs prior to incubation by studying the respiratory differences between fertilized and unfertilized eggs using the Non-invasive Micro-test Technique(NMT).A customized micro-testing examination platform,NMT Egg Testing System(NMT-ETS)was constructed for the real-time monitoring of the intensity and rate of oxygen exchange between the egg and its external environment.The results from this study revealed that at room temperature,there is a significant difference in gas exchange rates between fertilized and unfertilized eggs.The results indicate that the oxygen flux of fertilized eggs exceed 20 pmol/(cm^(2)·s),whereas unfertilized eggs show a much lower oxygen flux.Based on the results,the NMT method can be used to effectively distinguish between fertilized and unfertilized chicken eggs.展开更多
基金Supported by Southuest China Rice Innovation System and Crop High-yielding Project by Science and Technology (2011BAD02A05) Transformation Project of Agricultural Fruits into Capitals (2006GB2F000256)+2 种基金Sichuan Academic Leaders Training FundSichuan Rice Cultivation Key ProjectSichuan Financial Distribution Project~~
文摘[Objective] The aim was to research relationship between SPAD decline index after full-heading stage （SDIFHS） and productivity of rice. fertilized with nitro- gen in order to provide theoretical and practical references for selection and breed- ing of rice varieties. [Method] From 2008 to 2010, 18 mid-season hybrid rice vari- eties were researched every year to explore relationship＇ between SDIFHS and pro- ductivity of rice fertilized with nitrogen. [Result] The productivity of rice fertilized with nitrogen was of extremely significant positive corretation with SDIFHS, because the higher SPAD decline index is, the higher LAI decline index and the transformation ratio of dry matter to spikes in overground plant would be. [Conclusion] The re- search established a new method to predict productivity of rice fertilized with nitro- gen based on SPAD decline index.
基金Supported by Scientific and Technological Development General Program of Beijing Municipal Education Commission(KM201412448004)~~
文摘In order to investigate the effect of temperature on hatching result of fertilized peacock eggs, they were hatched under conditions of varying temperature and constant temperature respectively. The result shows that the hatching rate in the varying temperature treatment group （Group Ⅰ） is higher than that in the constant temperature treatment group （Group Ⅱ）. It suggests that the varying temperature treatment （high temperature in the early period, medium temperature in the middle period, slightly lower temperature in the late period, and slightly higher temperature in the hulling period） is in line with the optimum temperature variation in the hatching of fertilized peacock eggs, contributing the hatching of fertilized peacock eggs.
基金Supported by the Twelfth Five-year Plan"Integration and Demonstration of Key Technology of Raising Fish in Heilongjiang"(2012BAD25B10)~~
文摘In order to screen the effective preventive drugs for saprolegniasis occurred during hatching of the fertilized eggs of golden trout, effects of two kinds of self-made Chinese herbal compounds（AB） and two kinds of chemical bactericides（peroxyacetic acid andhydrogen peroxide, CD） with three disinfection concentrations were compared in this study. The fertilized eggs of golden trout were randomly divided into 13 groups, each group with two parallels of 1 000 eggs. Drugs at high,medium and low concentration were respectively used to immerse the fertilized eggs35 min for disinfection once a day. The numbers of live and dead eggs were recorded each day before disinfection and the numbers of survival eyed eggs were counted in each experimental group after finishing the whole test. The results indicated that all of four kinds of drugs used here had a certain effect on prevention and control of saprolegniasis during hatching of fertilized eggs of golden trout, and also showed a correlation between the concentrations and effects. After the selection of several drugs, we found that group B-medium, C-medium and D-high exhibited the most remarkable effects on control of saprolegniasis. However, careful consideration should be given to chemical drugs residues and their potential hazard to the eyed eggs. Therefore, Chinese herbal compound B was suggested to be used for prevention and control of saprolegniasis to achieve the healthy aquaculture. And the concentration and operation time of Chinese herbal compound B should be strictly controlled when used in disinfection of fertilized eggs.
基金supported by the grants from the NextGeneration BioGreen 21 Program(Nos.PJ009080 and PJ00909801)Rural Development Administration,Republic of Korea
文摘MicroRNAs（miRNAs） are a class of highly conserved small non-coding RNA molecules that play a pivotal role in several cellular functions.In this study,miRNA and messenger RNA（mRNA） profiles were examined by Illumina microarray in mouse embryonic stem cells（ESCs） derived from parthenogenetic,androgenetic,and fertilized blastocysts.The global analysis of miRNA-mRNA target pairs provided insight into the role of miRNAs in gene expression.Results showed that a total of 125 miRNAs and 2394 mRNAs were differentially expressed between androgenetic ESCs（aESCs） and fertilized ESCs（fESCs）,a total of 42 miRNAs and 87 mRNAs were differentially expressed between parthenogenetic ESCs（pESCs） and fESCs,and a total of 99 miRNAs and 1788 mRNAs were differentially expressed between aESCs and pESCs.In addition,a total of 575,5 and 376 miRNA-mRNA target pairs were observed in aESCs vs.fESCs,pESCs vs.fESCs,and aESCs vs.pESCs,respectively.Furthermore,15 known imprinted genes and 16 putative uniparentally expressed miRNAs with high expression levels were confirmed by both microarray and real-time RT-PCR.Finally,transfection of miRNA inhibitors was performed to validate the regulatory relationship between putative maternally expressed miRNAs and target mRNAs. Inhibition of miR-880 increased the expression of Peg3,Dyrklb,and Prrg2 mRNA,inhibition of miR-363 increased the expression of Nfat5 and Soatl mRNA,and inhibition of miR-883b-5p increased Nfat5,Tacstd2,and Ppapdc1 mRNA.These results warrant a functional study to fully understand the underlying regulation of genomic imprinting in early embryo development.
文摘This study aims to evaluate the production cost of Heterobranchus longifilis larvae reared in fertilized fish ponds. Experiment was carried out in two fish ponds. Each fish pond was filled with 200 liters of water and fertilized with dry poultry droppings (dose: 0.6 g.L-1) and zooplankton was inoculated with 83±13 individuals/L. Six days after inoculation of zooplankton, larvae of H. longifilis of 2 days after post-hatching were directly introduced into ponds with 300 individuals/m3. These larvae used exclusively zooplankton during the first 7 days. From the 8th day, larvae were fed with Coppens feed until 30 days. At the end, the survival rates were ranged between 58.33% and 56.67%. Final mean weights were brought up to all the ponds and were notsignificantly different (p > 0.05). The production costs ranged between 2.55 FCFA and 2.60 FCFA per fingerling with ratio production cost/weight gain around 0.80 FCFA/g in each pond. Then, this production system of fingerlings in fertilized ponds is very efficient and could be popularized in the rural fish farms.
基金Supported by the National Natural Science Foundation of China (No.30971869)the Knowledge Innovation Program of the Chinese Academy of Sciences (No.KZCX2-YW-438-1)
文摘Biologically active soil organic carbon （BASOC） is an important fraction of soil organic carbon （SOC）, but our understanding of the correlation between BASOC and soil aggregate stability is limited. At an ecological experimental station （28° 04＇-28° 37＇ N, 116° 41＇-117° 09＇ E） in Yujiang County, Jiangxi Province, China, we analyzed the dynamic relationship between soil aggregate stability and BASOC content over time in the red soil （Udic Ferrosols） fertilized with a nitrogen-phosphorus-potassium chemical fertilizer （NPK） without manure or with NPK plus livestock manure or green manure. The dynamics of BASOC was evaluated using CO2 efflux, and soil aggregates were separated according to size using a wet-sieving technique. The soils fertilized with NPK plus livestock manure had a significantly higher content of BASOC and an improved aggregate stability compared to the soils fertilized with NPK plus green manure or NPK alone. The BASOC contents in all fertilized soils decreased over time. The contents of large aggregates （800-2 000 μm） dramatically decreased over the first 7 d of incubation, but the contents of small aggregates （〈 800 μm） either remained the same or increased, depending on the incubation time and specific aggregate sizes. The aggregate stability did not differ significantly at the beginning and end of incubation, but the lowest stability in all fertilized soils occurred in the middle of the incubation, which implied that the soils had a strong resilience for aggregate stability. The change in BASOC content was only correlated with aggregate stability during the first 27 d of incubation.
基金supported by the National Natural Science Foundation of China (Nos. 41425005 and 41571130062)
文摘The production of nitrous oxide (N2O) is a widespread trait in fungi and is of interest because denitrifying fungi lack the N2O reductase gene (nosZ) that regulates N2O reduction to nitrogen gas (N2). The adaptive ability of soil fungi is better than that of bacteria in acidic soils. We investigated the N2O reduction potential, described by the N2O product ratio (Nn2O), N2O/(N2O+N2), in soils of different types of fields under crop cultivation with different fertilizer inputs and a bare fallow field with no fertilization as a control. The fungi-to-bacteria abundance ratio (Rf/b) was negatively correlated (P < 0.01) with the natural pH of the soil;however, the high value of Rp/b measured in vineyards was due to the large inputs of manure. When the denitrification potential was measured at natural pH values of soils, Nn2O was negatively correlated (P < 0.01) with soil pH. When the denitrification potential was measured after short-term modifications of soil pH, however, no significant correlation was found between Nn2O and the modified pH. Based on stepwise multiple regression analysis, soil pH and residual nitrate (NO3) were the key factors regulating N2O reduction in soils at natural pH values (R^2 = 0.8& P < 0.001), whereas the key factor was the soil residual NO, alone (R^2 = 0.83, P < 0.001) when the soil pH was modified. When the effect of the soil chemical properties was weakened, a high Rf/b value had the potential (P < 0.01) to affect N2O reduction;however, the role of fungi was offset by the presence of denitrifying bacteria. These results provide evidence that compared to the indirect effects of Rf/b、the direct effects of the soil chemical properties have a greater effect on N2O reduction in fertilized soils.
基金the National Key Technology R&D Program of China(Nos.2012BAD15B05 and 2013BAD16B01)
文摘Fertilizers are widely applied in agricultural practice to improve crop yield and quality.However, they can also alter the behavior of soil pollutants.A field experiment was conducted on a chernozem in Heilongjiang Province, China.Various proportions of inorganic nitrogen(N), phosphorus(P), and organic(manure) fertilizers were applied.Soil samples were collected four times over 2 years, and the total concentrations and speciation of chromium(Cr) and cadmium(Cd) were analyzed.Inorganic fertilizer addition had little effect on the total concentration of Cr and Cd, while organic fertilizer addition reduced the total concentrations of both Cr and Cd.Inorganic fertilizers increased the concentration of Cr in the exchangeable form, but decreased that of the residual form(P < 0.05).Addition of inorganic or organic fertilizer alone decreased concentrations of exchangeable Cd(P < 0.05), but the combination of inorganic and organic fertilizers had the opposite effect.High concentration of total Cd exceeding the upper limits of the Environmental Quality Standard for Soils proposed by the Ministry of Environmental Protection of China could result in an environmental risk, and fertilizer application could lower that risk.Results of risk assessment code calculated as the percentage of the sum of metal concentrations in the exchangeable and carbonate-associated form in total metal concentration showed that Cr and Cd risks were lowered when organic fertilizer was applied alone or in combination with inorganic fertilizers.Our study highlighted that organic fertilizer should be applied alone or in combination with inorganic fertilizers to lower the environmental risks of Cr and Cd pollution in the chernozem.
基金supported by Xiamen Science and Technology Innovation Platform Funded Program (3502Z20111006)
文摘This article presents a case of conservative managements of an in vitro-fertilized twin pregnancy with early loss of the first fetus. A 37-year-old woman in the 21st week of a twin pregnancy delivered her one of the fetuses with unfortunate outcome after the premature rupture of membranes. The umbilical cord of the first fetus was ligated at the cervical level and the second surviving fetus was left in utero. In order to save the surviving fetus, conservative managements including combination of steroids, tocolytics, antibiotics and cervical cerclage were performed. The second fetus was delivered vaginally in the 27th week of pregnancy, 4 h after premature rupture of membranes of the remaining amniotic sac, 41 d after the first fetus was born. The child and the mother have been followed up for 4 years and they are healthy. Conservative managements including steroids, tocolytics, antibiotics and cervical cerclage in a closely monitored environment would be given following the delivery of the first fetus. Delayed-interval pregnancy appears to be safe for the mother and the remaining fetuses.
基金financially supported by the Science and Technology Major Project of the Inner Mongolia Autonomous Region of China(2020ZD0007)the Major Program of the Inner Mongolia Natural Science Foundation,China(2020ZD10)+3 种基金the National Natural Science Foundation of China(32160172)the Natural Science Foundation of Inner Mongolia Autonomous Region(2020BS03003 and 2020BS03022)the National Transgenic Project of China(2016ZX0801000-002 and 2016ZX08010005-001)the Science and Technology Major Project of the Inner Mongolia Autonomous Region of China(zdzx2018065)。
文摘Lysophosphatidic acid(LPA)is a small molecule glycerophospholipid,which regulates multiple downstream signalling pathways through G-protein-coupled receptors to achieve numerous functions on oocyte maturation and embryo development.In this study,sheep in vitro fertilized embryos were applied to investigate the effects of LPA on early embryos development and embryonic stem cell establishment.At first,the maturation medium containing estrus female sheep serum and synthetic oviduct fluid(SOF)were optimized for sheep IVF,and then the effects of LPA were investigated.From 0.1 to 10μmol L^(–1),LPA had no significant effect on the cleavage rate(P>0.05),but the maturation rate and blastocyst rate increased dependently with LPA concentration(P<0.05),and the blastocyst morphology was normal.When the LPA concentration was 15μmol L^(–1),the maturation rate,cleavage rate and blastocyst rate decreased significantly(P<0.05),and the blastocyst exhibited abnormal morphology and could not develop into highquality blastocyst.Besides,the exogenous LPA increases the expression of LPAR2,LPAR4,TE-related gene CDX-2and pluripotency-related gene OCT-4 in sheep early IVF embryos with the raise of LPA concentration from 0.1 to 10μmol L^(–1).The expression of LPAR2,LPAR4,CDX-2 and OCT-4 from the LPA-0.1μmol L^(–1)to LPA-10μmol L^(–1)groups in early embryos were extremely significant(P<0.05),while the expression of these genes significantly decreased in 15μmol L^(–1)LPA-treated embryos compared with LPA-10μmol L^(–1)group(P<0.05).The inner cell mass in 15μmol L^(–1)LPA-treated embryos was also disturbed,and the blastocysts formation was abnormal.Secondly,the sheep IVF blastocysts were applied to establish embryonic stem cells.The results showed that LPA made the blastocyst inoculated cells grow towards TSC-like cells.They enhanced the fluorescence intensity and mRNA abundance of OCT-4 and CDX-2 as the concentration increased from 0 to 10μmol L^(–1),while 15μmol L^(–1)LPA decreased OCT-4 and CDX-2 expression in the derived cells.The expression of CDX-2 and OCT-4 in the blastocyst inoculated cells of LPA-1μmol L^(–1)group and LPA-10μmol L^(–1)group extremely significantly increased(P<0.05),but there was significant decrease in LPA-15μmol L^(–1)group compared with LPA-10μmol L^(–1)group(P<0.05).Meanwhile,the protein expression of LPAR2 and LPAR4 remarkably increased after treatment of LPA at 10μmol L^(–1)concentration.This study references the IVF embryo production and embryonic stem cell research of domestic animals.
文摘We compared chemical and biological properties of soils in organically and conventionally fertilized apple orchards in Nagano Prefecture (one of the major apple producing regions in Japan). Five apple orchards with different fertilizer management systems were used for this study. The total carbon and total nitrogen contents were higher in the organically fertilized orchard, while the total phosphorus and total potassium were at similar levels in both organically and conventionally fertilized orchards. The bacterial biomass did not differ between the two orchards, but the N circulation activity was clearly higher in the organically fertilized orchard from April to December. Total carbon from 50,000 to 60,000 mg/kg, total nitrogen at about 3000 to 4000 mg/kg, and a C/N ratio of 15 - 20 were suggested to be suitable conditions for a high level of apple production under an organic fertilizer management system.
文摘To study the sensitivity of inter-subspecific hybrid rice to climatic conditions, the spikelet fertilized rate （SFR） of four types of rice including indica-japonica hybrid, intermediate hybrid, indica and japonica were analyzed during 2000-2004. The inter-subspecific hybrids showed lower SFR, and much higher fluctuation under various climatic conditions than indica and japonica rice, showing the inter-subspecific hybrids were sensitive to ecological conditions. Among 12 climatic factors, the key factor affecting rice SFR was temperature, with the most significant factor being the average temperature of the seven days around panicle flowering （T7）. A regressive equation of SFR-temperature by T7, and a comprehensive synthetic model by four important temperature indices were put forward. The optimum temperature for inter-subspecific hybrids was estimated to be 26.1-26.6℃, and lower limit of safe temperature to be 22.5-23.3℃ for panicle flowering, showing higher by averagely 0.5℃ and 1.7℃, respectively, to be compared with indica and japonica rice. This suggested that inter-subspecific hybrids require proper climatic conditions. During panicle flowering, the suitable daily average temperature was 23.3-29.0℃, with the fittest one at 26.1-26.6℃. For an application example, optimum heading season for inter-subspecific hybrids in key rice growing areas in China was as same as common pure lines, while inferior limit for safe date of heading was about a ten-day period earlier than those of common pure lines.
文摘Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of preimplantation embryos. ESCs exhibit true pluripotency, i.e., the ability to differentiate into cells of all three germ layers in the developing embryo. We used 2-DE MALDI-TOF/TOF to identify differentially expressed proteins among three types of mouse embryonic stem cells (ESCs) derived from ferti-lized, parthenogenetic, and androgenetic (fESC, pESC and aESC, respectively) blastocysts. We detected more than 800 proteins on silver- stained gels of whole protein extracts from each type of ESC. Of these, 52 differentially expressed proteins were identified by the MALDI–TOF/TOF analyzer, including 32 (fESCs vs. pESCs), 28 (fESCs vs. aESCs) and 17 (pESCs vs. aESCs) prominent proteins with significantly higher or lower expression relative to the comparison cells. Among the 32 proteins from fESCs, 12 were significantly increased in expression and 20 were reduced in expression in fESCs com-pared with pESCs. Similarly, 10 of 28 and 8 of 17 proteins were more highly expressed in fESCs and pESCs compared with aESCs, respectively. In contrast, 18 of 28 and 9 of 17 proteins were reduced in expression in fESCs and pESCs compared with aESCs, respectively. Of the eight protein candidates in fESCs, four were in-creased and four were decreased in expression relative to both pESCs and aESCs in the 2-DE analysis. Differential expression of these pro-teins were confirmed by mRNA expression analysis using real time RT-PCR. For three pro-teins, ANXA5, CLIC1 and SRM, Western blot analysis corroborated the expression patterns indicated by the 2-DE results. ANXA5 and CLIC1 were increased in expression and SRM was de-creased in expression in fESCs compared with both pESCs and aESCs. The differentially ex-pressed proteins identified in the present study warrant further investigation towards the goal of their potential application in ESC therapy.
文摘Objective: To determine the effect of preparation to play a maternal role in mothers fertilized through in vitro fertilization on maternal anxiety. Methods: A total of 60 mothers undergoing in vitro fertilization were assigned into intervention group and control group. Pregnancy concerns and stress questionnaires were research tools. Questionnaires were completed in both groups before organizing preparation program. Then, the preparation program was held for four sessions for the mothers in the intervention group, and the subjects in both groups were immediately investigated again after completion of preparation program and one month later. Data analyses about pregnancy stress and concerns of mothers and its dimensions were performed by repeated measure (analysis of variance), Mann-Whitney, Friedman and Wilcoxon tests. Analysis of demographic variables was performed by using independent t-test and Chi-square test in SPSS 21. Results: The score of pregnancy stress and concerns before the intervention was not significantly different between the two groups. There was a significant difference in the intervention group in the score of pregnancy stress and concerns before and after the intervention (P<0.001), before and one month after the intervention (P<0.001), immediately after and one month after the intervention (P<0.001) which was not significant in the control group. Conclusions: Maternal preparation program can be effective in reducing maternal stress and concerns.
文摘In this experiment,the sperms were obtained from(C57×CBA) F1 male mice and the eggs from NIHstrain female mice.Using the fertilization techni-que in Vitro,the slides of chromosome of mouse eggsat the first cleavage metaphase were prepared.
文摘The objective of this study was to examine the effects of addition of inoculant to high-nitrogen (N) fertilized timothy on fermentation, nutritive value, and feed intake of silage. The silage of timothy cultivated with a N fertilizer rate at two levels (high level (H), standard level (S)) was prepared with (SI, HI) or without (SC, HC) an inoculants. The CP content of H increased by 38 g·kg-1 DM compared with that of S, and the WSC and ADF contents decreased compared with those of S. Regarding the fermentation of silage, the pH and NH3-N ratio was significantly lower in the silage with inoculant (SI and HI), showing improvement of the fermentation compared with those of the silage without the addition (SC and HC). The CP content was significantly higher in HC and HI than in SC and SI, and the NDF content was the lowest in HI among the four treatments. The CP digestibility of HC and HI was significantly higher than those of SC and SI. The EE digestibility of SI was significantly higher than that of SC, and that of HI was significantly higher than that of HC. The DCP contents were significantly higher in HC and HI than in SC and SI. The TDN content was the lowest in SC and highest in HI. The DCP intakes of HC and HI were significantly higher than those of SC and SI. When the N fertilizer rate was increased, the DCP content and DCP intake of the silage increased, and the addition of inoculant improved the fermentation and increased the TDN content.
文摘Apple/Malus x domestica cv. ＇Topaz＇/M26 and sour cherry cv. ＇Debreceni Botermo＇/Prunus mahaleb L. maidens were fertilized with different organic fertilizers and soil amendments in an organic nursery. A mycorrhizal inoculant, Micosat, was added to the non-fertilized control and to combinations fertilized with various bioproducts. Under assessment was the effect of nutrient delivery on the growth and development of maiden fruit trees focussing on tree height, thickness of the tree trunk, the number and the length of lateral shoots in each treatment combination. It was found that the introduction of mycorrhizal fungi into the soil without fertilizer application significantly improved the growth and development of apple and sour cherry maidens. Mineral fertilizer application （NPK） combined with mycorrhizal inoculation had a negligible effect on the above features. In the presence of bioproducts such as granular manure, Humus Active ＋ Aktywit PM, BF Amin, Vinassa and Florovit Eko, the mycorrhizal fungi were more biologically active than in the absence of these products.
文摘Coral reef restoration approaches have often utilized adult colonies as sources for fragments (i.e. clones) to be transplanted. Although restoration through this method is fast and cheap, it has been pointed out that it may reduce genetic diversity of the restored population. Low genetic diversity is a concern for reef restoration when seed fragments are raised asexually from only a few donor colonies. This can lead to lower fertilization rates among seed fragments, and reducing the longterm benefits of reef restoration in particular areas. Additionally, low genetic diversity can compound the effects of increased ocean temperature and other environmental stressors, further jeopardizing the health of a reef. An alternative approach through sexually propagated coral cultures and out-plantings can alleviate this problem. Sexually produced offsprings are more genetically diverse. They can be produced in far greater numbers than coral fragments and do not imply destructive methods. Ongoing research at the Akajima Marine Science Laboratory in Okinawa, Japan has helped to improve the production and maintenance of sexually propagated larval cultures. Our results show that crosses between gametes from 6 or more colonies will provide the highest fertilization rate (>95%). Based on the results, we suggest the use of 6 or more donor colonies for practical gamete fertilization in sexually derived coral culture.
文摘Rationale:This case report describes a couple with recurrent fertilization failure despite undergoing multiple cycles of intracytoplasmic sperm injection(ICSI).The principal clinical concern was suspected oocyte activation deficiency(OAD),in which fertilization is impeded due to the oocyte’s inability to initiate embryogenesis,commonly attributed to inadequate intracellular calcium(Ca^(2+))release following sperm injection.Patient concerns:The couple repeatedly experienced complete or near-complete fertilization failure in previous ICSI cycles,raising suspicion of an underlying oocyte activation defect.Diagnosis:Based on the repeated absence of fertilization post-ICSI and clinical history,a diagnosis of suspected OAD leading to recurrent ICSI fertilization failure was considered.Interventions:Artificial oocyte activation(AOA)using the calcium ionophore A23187 was performed.After ICSI,unfertilized oocytes were exposed to the ionophore to induce Ca^(2+)influx,simulating physiological calcium oscillations essential for oocyte activation.The efficacy of intervention was evaluated through subsequent embryonic development,morphological grading,and chromosomal integrity.Outcomes:Following AOA treatment,successful oocyte activation occurred,resulting in the formation of high-grade embryos with normal developmental progression.Chromosomal analysis revealed no detectable abnormalities,indicating genomic stability.Lessons:Calcium ionophore–mediated AOA may serve as an effective adjunct in cases of recurrent ICSI failure attributed to OAD.This case highlights the importance of individualized therapeutic strategies in assisted reproduction;however,further research is needed to refine protocols,validate broader clinical efficacy,and assess long-term safety,including potential epigenetic risks.
基金supported by National Natural Science Foundation of China(31371771)Special Fund for Agroscientific Research in the Public Interest(201303084)National Science and Technology Support Programme(2015BAD19B05).
文摘One unresolved challenge in the egg industry is how to efficiently and non-invasively detect unfertilized eggs prior to incubation.This detection ability would not only significantly improve hatching rates and reduce costs but also conserve incubator space and prevent poor-quality embryos from contributing to the spread of infections.This study demonstrates a procedure for distinguishing between fertilized and unfertilized eggs prior to incubation by studying the respiratory differences between fertilized and unfertilized eggs using the Non-invasive Micro-test Technique(NMT).A customized micro-testing examination platform,NMT Egg Testing System(NMT-ETS)was constructed for the real-time monitoring of the intensity and rate of oxygen exchange between the egg and its external environment.The results from this study revealed that at room temperature,there is a significant difference in gas exchange rates between fertilized and unfertilized eggs.The results indicate that the oxygen flux of fertilized eggs exceed 20 pmol/(cm^(2)·s),whereas unfertilized eggs show a much lower oxygen flux.Based on the results,the NMT method can be used to effectively distinguish between fertilized and unfertilized chicken eggs.
