Panax species are globally recognized for their high medicinal and economic value,yet large-scale cultivation is constrained by high production costs,progressive soil acidification,and persistent soil-borne diseases.A...Panax species are globally recognized for their high medicinal and economic value,yet large-scale cultivation is constrained by high production costs,progressive soil acidification,and persistent soil-borne diseases.Although various soil improvement strategies have been tested,a comprehensive synthesis of their comparative effectiveness has been lacking.Here,we conducted a meta-analysis of 1381 observations from 54 independent studies to evaluate the effects of conventional fertilizers,microbial fertilizers,organic amendments,and inorganic amendments on Panax cultivation.Our results demonstrate that microbial fertilizers,organic amendments,and inorganic amendments significantly increased soil pH,thereby ameliorating soil acidification.Among them,organic amendments significantly enhanced the content of soil organic carbon,available nitrogen,and available phosphorus,alongside a notable increase in microbial diversity(Chao1 and ACE indices,which increased by 9%and 17%,respectively).Moreover,our analysis revealed that while microbial fertilizers,organic amendments,and inorganic amendments(except conventional fertilizers)reduced the disease index of Panax plants,organic amendments demonstrated absolute superiority in promoting plant height,root dry weight,root fresh weight,and root length.By quantitatively integrating multi-source evidence,this study provides novel mechanistic insights and practical recommendations that extend beyond local practices,offering guidance for sustainable ginseng cultivation and broader medicinal plant production systems worldwide.展开更多
Mammalian fertilization involves the migration of spermatozoa through the female reproductive system.Early embryonic development is a consequence of several steps and signaling pathways being activated,as well as bioc...Mammalian fertilization involves the migration of spermatozoa through the female reproductive system.Early embryonic development is a consequence of several steps and signaling pathways being activated,as well as biochemical and morphological modifications of spermatozoa that enable them to penetrate the membrane of mature oocytes.There are some crucial steps known to clearly explain the process of fertilization,starting with hyperactivation of spermatozoa,mutual recognition,and binding of gametes mediated by receptors located on the surface membranes of both gametes.The final step is followed by oocyte activation,which is primarily triggered via sperm-derived factors,inducing a sharp increase in intracellular calcium levels,eventually leading to polyspermy block.This review integrates current knowledge of the molecular and physiological events governing fertilization,emphasizing how ion regulation and signaling pathways converge to enable sperm function and oocyte activation.Special attention is given to sperm-derived factors such as phospholipase C zeta(PLCζ)and post-acrosomal sheath WW domain-binding protein(PAWP),which play essential roles in triggering calcium release and supporting early embryonic development.展开更多
Rationale:This case report describes a couple with recurrent fertilization failure despite undergoing multiple cycles of intracytoplasmic sperm injection(ICSI).The principal clinical concern was suspected oocyte activ...Rationale:This case report describes a couple with recurrent fertilization failure despite undergoing multiple cycles of intracytoplasmic sperm injection(ICSI).The principal clinical concern was suspected oocyte activation deficiency(OAD),in which fertilization is impeded due to the oocyte’s inability to initiate embryogenesis,commonly attributed to inadequate intracellular calcium(Ca^(2+))release following sperm injection.Patient concerns:The couple repeatedly experienced complete or near-complete fertilization failure in previous ICSI cycles,raising suspicion of an underlying oocyte activation defect.Diagnosis:Based on the repeated absence of fertilization post-ICSI and clinical history,a diagnosis of suspected OAD leading to recurrent ICSI fertilization failure was considered.Interventions:Artificial oocyte activation(AOA)using the calcium ionophore A23187 was performed.After ICSI,unfertilized oocytes were exposed to the ionophore to induce Ca^(2+)influx,simulating physiological calcium oscillations essential for oocyte activation.The efficacy of intervention was evaluated through subsequent embryonic development,morphological grading,and chromosomal integrity.Outcomes:Following AOA treatment,successful oocyte activation occurred,resulting in the formation of high-grade embryos with normal developmental progression.Chromosomal analysis revealed no detectable abnormalities,indicating genomic stability.Lessons:Calcium ionophore–mediated AOA may serve as an effective adjunct in cases of recurrent ICSI failure attributed to OAD.This case highlights the importance of individualized therapeutic strategies in assisted reproduction;however,further research is needed to refine protocols,validate broader clinical efficacy,and assess long-term safety,including potential epigenetic risks.展开更多
The combined effects of macronutrients(Nitrogen,Phosphorus,and Potassium-N,P,K)and micronutrient fertilization on turmeric yield,soil enzymatic activity,microbial biomass,and nutrient dynamics remains poorly understoo...The combined effects of macronutrients(Nitrogen,Phosphorus,and Potassium-N,P,K)and micronutrient fertilization on turmeric yield,soil enzymatic activity,microbial biomass,and nutrient dynamics remains poorly understood,despite their significance for sustainable soil fertility management and optimizing crop productivity across diverse agroecosystems.To investigate,a net house experiment on sandy loam Haplic Chernozem was conducted to 03 fertilizer regimes,viz.N_(75)P_(50)K_(50)kg ha^(−1)(T-2),N_(12)5P_(100)K_(100)kg ha^(−1)(T-3),and N_(100)P_(75)K_(75)+B_(3)Zn_(6)Fe_(6)kg ha^(−1)(T-4).Furthermore,the influence of these treatments was systematically assessed on soil nutrient status(N,P,K),enzymatic activities(alkaline phosphomonoesterase,dehydrogenase,fluorescein diacetate hydrolysis),microbial biomass carbon(MBC)and soil organic carbon(SOC).Balanced fertilization significantly turmeric productivity and soil health.All three fertilizer treatments showed a clear yield increase compared to the unfertilized control.Compared to the control,N_(75)P_(50)K_(50)kg/ha T-2 increased rhizome number and biomass per plant by 44.7%and 16.3%,respectively,while N_(100)P_(75)K_(75)+B_(3)Zn_(6)Fe_(6)kg/ha T-4 further enhanced them by 86.6%and 27.7%.T-3 produced the most significant yield response by increasing the rhizome biomass by 38.0%and rhizome number per plant by 100%compared to the control.The nutrient availability was also substantially improved.T-2 enhanced the soil nitrogen contents by 83.3%with maximum N levels observed in T-3&T-4.Phosphorus increased by 61.5%in T-3 and 37.3%in T-4,while potassium was enhanced by 12.9%in T-3 relative to the control,respectively.Enzymatic activities were markedly enhanced as T-3 was recorded to improve alkaline phosphomonoesterase(APA),dehydrogenase(DHA)and fluorescein diacetate hydrolysis(FDA)by 50.6%,37.4%,and 43.4%,where T-4 increased by 32.2%,30.9%,and 35.9%,respectively compared to control.MBC and SOC also rose significantly,with SOC increased by 13.8%(T-2),41.6%(T-3),and 47.2%(T-3)relative to control.The result of this study demonstrates that the integrated macroµnutrient fertilization,particularly T-37 T-4 treatments,sustainably enhanced turmeric yield,soil nutrient availability,enzyme activity,microbial biomass,and organic carbon.These findings highlight the critical role of balanced nutrient management in sustaining soil fertility and crop productivity across agroecosystems.展开更多
目的:探讨Guided Care护理模式在不孕症体外受精-胚胎移植(In vitro fertilization and embryo transfer,IVFET)助孕患者中的应用效果。方法:选取2022年12月至2024年12月于本院接受IVF-ET助孕治疗的92例不孕症患者作为研究对象,采用随...目的:探讨Guided Care护理模式在不孕症体外受精-胚胎移植(In vitro fertilization and embryo transfer,IVFET)助孕患者中的应用效果。方法:选取2022年12月至2024年12月于本院接受IVF-ET助孕治疗的92例不孕症患者作为研究对象,采用随机数字表法分为对照组和观察组,各46例。对照组患者接受常规护理,观察组患者接受Guided Care护理模式,两组均持续护理2 m。比较两组心理状态、治疗依从性、生活质量以及护理满意度。结果:护理后,观察组抑郁-焦虑-压力量表(Depression Anxiety Stress Scales,DASS)各项评分均较对照组低,Morisky改良版服药依从性量表(Morisky Medication Adherence Scale,MMAS-8)评分、(The Mos 36-item Short Form Health Survey,SF-36)评分及护理满意度均高于对照组(P<0.05)。结论:Guided Care护理模式能够有效改善不孕症患者接受IVF-ET治疗期间的焦虑、抑郁情绪,增强其治疗依从性,对于顺利妊娠具有积极意义,从而获得更高的护理满意度。展开更多
The aim of this study was to investigate whether the sperm chromatin structure assay （SCSA） results after swim-up are related to fertilization rates, embryo quality and pregnancy rates following in vitrofertilizati...The aim of this study was to investigate whether the sperm chromatin structure assay （SCSA） results after swim-up are related to fertilization rates, embryo quality and pregnancy rates following in vitrofertilization （IVF）. A total of 223 couples undergoing IVF in our hospital from October 2008 to September 2009 were included in this study. Data on the IVF process and sperm chromatin structure assay results were collected. Fertilization rate, embryo quality and IVF success rates of different DNA fragmentation index （DFI） subgroups and high DNA stainability （HDS） subgroups were compared. There were no significant differences in fertilization rate, clinical pregnancy or delivery rates between the DFI and HDS subgroups. However, the group with abnormal DFI had a lower good embryo rate. So, we concluded that the SCSA variables, either DFI or HDS after swim-up preparation, were not valuable in predicting fertilization failure or pregnancy rate, but an abnormal DFI meant a lower good embryo rate following IVF.展开更多
基金supported by the National Natural Science Foundation of China(Nos.42388101,82403271)Natural Science Foundation of Ningbo Municipality(No.2024J420)Ningbo Top Talent Project(No.215-432094250).
文摘Panax species are globally recognized for their high medicinal and economic value,yet large-scale cultivation is constrained by high production costs,progressive soil acidification,and persistent soil-borne diseases.Although various soil improvement strategies have been tested,a comprehensive synthesis of their comparative effectiveness has been lacking.Here,we conducted a meta-analysis of 1381 observations from 54 independent studies to evaluate the effects of conventional fertilizers,microbial fertilizers,organic amendments,and inorganic amendments on Panax cultivation.Our results demonstrate that microbial fertilizers,organic amendments,and inorganic amendments significantly increased soil pH,thereby ameliorating soil acidification.Among them,organic amendments significantly enhanced the content of soil organic carbon,available nitrogen,and available phosphorus,alongside a notable increase in microbial diversity(Chao1 and ACE indices,which increased by 9%and 17%,respectively).Moreover,our analysis revealed that while microbial fertilizers,organic amendments,and inorganic amendments(except conventional fertilizers)reduced the disease index of Panax plants,organic amendments demonstrated absolute superiority in promoting plant height,root dry weight,root fresh weight,and root length.By quantitatively integrating multi-source evidence,this study provides novel mechanistic insights and practical recommendations that extend beyond local practices,offering guidance for sustainable ginseng cultivation and broader medicinal plant production systems worldwide.
文摘Mammalian fertilization involves the migration of spermatozoa through the female reproductive system.Early embryonic development is a consequence of several steps and signaling pathways being activated,as well as biochemical and morphological modifications of spermatozoa that enable them to penetrate the membrane of mature oocytes.There are some crucial steps known to clearly explain the process of fertilization,starting with hyperactivation of spermatozoa,mutual recognition,and binding of gametes mediated by receptors located on the surface membranes of both gametes.The final step is followed by oocyte activation,which is primarily triggered via sperm-derived factors,inducing a sharp increase in intracellular calcium levels,eventually leading to polyspermy block.This review integrates current knowledge of the molecular and physiological events governing fertilization,emphasizing how ion regulation and signaling pathways converge to enable sperm function and oocyte activation.Special attention is given to sperm-derived factors such as phospholipase C zeta(PLCζ)and post-acrosomal sheath WW domain-binding protein(PAWP),which play essential roles in triggering calcium release and supporting early embryonic development.
文摘Rationale:This case report describes a couple with recurrent fertilization failure despite undergoing multiple cycles of intracytoplasmic sperm injection(ICSI).The principal clinical concern was suspected oocyte activation deficiency(OAD),in which fertilization is impeded due to the oocyte’s inability to initiate embryogenesis,commonly attributed to inadequate intracellular calcium(Ca^(2+))release following sperm injection.Patient concerns:The couple repeatedly experienced complete or near-complete fertilization failure in previous ICSI cycles,raising suspicion of an underlying oocyte activation defect.Diagnosis:Based on the repeated absence of fertilization post-ICSI and clinical history,a diagnosis of suspected OAD leading to recurrent ICSI fertilization failure was considered.Interventions:Artificial oocyte activation(AOA)using the calcium ionophore A23187 was performed.After ICSI,unfertilized oocytes were exposed to the ionophore to induce Ca^(2+)influx,simulating physiological calcium oscillations essential for oocyte activation.The efficacy of intervention was evaluated through subsequent embryonic development,morphological grading,and chromosomal integrity.Outcomes:Following AOA treatment,successful oocyte activation occurred,resulting in the formation of high-grade embryos with normal developmental progression.Chromosomal analysis revealed no detectable abnormalities,indicating genomic stability.Lessons:Calcium ionophore–mediated AOA may serve as an effective adjunct in cases of recurrent ICSI failure attributed to OAD.This case highlights the importance of individualized therapeutic strategies in assisted reproduction;however,further research is needed to refine protocols,validate broader clinical efficacy,and assess long-term safety,including potential epigenetic risks.
基金supported by the Department of Biotechnology,Government of India(DBT),TWAS,and the Ministry of Innovative Development of Uzbekistan。
文摘The combined effects of macronutrients(Nitrogen,Phosphorus,and Potassium-N,P,K)and micronutrient fertilization on turmeric yield,soil enzymatic activity,microbial biomass,and nutrient dynamics remains poorly understood,despite their significance for sustainable soil fertility management and optimizing crop productivity across diverse agroecosystems.To investigate,a net house experiment on sandy loam Haplic Chernozem was conducted to 03 fertilizer regimes,viz.N_(75)P_(50)K_(50)kg ha^(−1)(T-2),N_(12)5P_(100)K_(100)kg ha^(−1)(T-3),and N_(100)P_(75)K_(75)+B_(3)Zn_(6)Fe_(6)kg ha^(−1)(T-4).Furthermore,the influence of these treatments was systematically assessed on soil nutrient status(N,P,K),enzymatic activities(alkaline phosphomonoesterase,dehydrogenase,fluorescein diacetate hydrolysis),microbial biomass carbon(MBC)and soil organic carbon(SOC).Balanced fertilization significantly turmeric productivity and soil health.All three fertilizer treatments showed a clear yield increase compared to the unfertilized control.Compared to the control,N_(75)P_(50)K_(50)kg/ha T-2 increased rhizome number and biomass per plant by 44.7%and 16.3%,respectively,while N_(100)P_(75)K_(75)+B_(3)Zn_(6)Fe_(6)kg/ha T-4 further enhanced them by 86.6%and 27.7%.T-3 produced the most significant yield response by increasing the rhizome biomass by 38.0%and rhizome number per plant by 100%compared to the control.The nutrient availability was also substantially improved.T-2 enhanced the soil nitrogen contents by 83.3%with maximum N levels observed in T-3&T-4.Phosphorus increased by 61.5%in T-3 and 37.3%in T-4,while potassium was enhanced by 12.9%in T-3 relative to the control,respectively.Enzymatic activities were markedly enhanced as T-3 was recorded to improve alkaline phosphomonoesterase(APA),dehydrogenase(DHA)and fluorescein diacetate hydrolysis(FDA)by 50.6%,37.4%,and 43.4%,where T-4 increased by 32.2%,30.9%,and 35.9%,respectively compared to control.MBC and SOC also rose significantly,with SOC increased by 13.8%(T-2),41.6%(T-3),and 47.2%(T-3)relative to control.The result of this study demonstrates that the integrated macroµnutrient fertilization,particularly T-37 T-4 treatments,sustainably enhanced turmeric yield,soil nutrient availability,enzyme activity,microbial biomass,and organic carbon.These findings highlight the critical role of balanced nutrient management in sustaining soil fertility and crop productivity across agroecosystems.
文摘目的:探讨Guided Care护理模式在不孕症体外受精-胚胎移植(In vitro fertilization and embryo transfer,IVFET)助孕患者中的应用效果。方法:选取2022年12月至2024年12月于本院接受IVF-ET助孕治疗的92例不孕症患者作为研究对象,采用随机数字表法分为对照组和观察组,各46例。对照组患者接受常规护理,观察组患者接受Guided Care护理模式,两组均持续护理2 m。比较两组心理状态、治疗依从性、生活质量以及护理满意度。结果:护理后,观察组抑郁-焦虑-压力量表(Depression Anxiety Stress Scales,DASS)各项评分均较对照组低,Morisky改良版服药依从性量表(Morisky Medication Adherence Scale,MMAS-8)评分、(The Mos 36-item Short Form Health Survey,SF-36)评分及护理满意度均高于对照组(P<0.05)。结论:Guided Care护理模式能够有效改善不孕症患者接受IVF-ET治疗期间的焦虑、抑郁情绪,增强其治疗依从性,对于顺利妊娠具有积极意义,从而获得更高的护理满意度。
基金ACKNOWLEDGMENTS This study was supported by grants from the Natural Science Foundation of Shanghai （No. 10JC1410800）. The support of Jian-Bin Liu in collecting the patient data is gratefully acknowledged.
文摘The aim of this study was to investigate whether the sperm chromatin structure assay （SCSA） results after swim-up are related to fertilization rates, embryo quality and pregnancy rates following in vitrofertilization （IVF）. A total of 223 couples undergoing IVF in our hospital from October 2008 to September 2009 were included in this study. Data on the IVF process and sperm chromatin structure assay results were collected. Fertilization rate, embryo quality and IVF success rates of different DNA fragmentation index （DFI） subgroups and high DNA stainability （HDS） subgroups were compared. There were no significant differences in fertilization rate, clinical pregnancy or delivery rates between the DFI and HDS subgroups. However, the group with abnormal DFI had a lower good embryo rate. So, we concluded that the SCSA variables, either DFI or HDS after swim-up preparation, were not valuable in predicting fertilization failure or pregnancy rate, but an abnormal DFI meant a lower good embryo rate following IVF.
