Some haplotypes of the sucrose synthase gene TaSus1 are associated with thousand-grain weight(TGW)in wheat(Triticum aestivum L.).However,no mutations have been identified within the gene to test this association.The e...Some haplotypes of the sucrose synthase gene TaSus1 are associated with thousand-grain weight(TGW)in wheat(Triticum aestivum L.).However,no mutations have been identified within the gene to test this association.The effects of TaSus1 on grain number per spike(GNS)also are largely unknown.Our previous genome-wide association study identified TaSus-A1 as a candidate gene controlling fertile spikelet number per spike(FSN).In the present study,we generated two independent mutants for the three TaSus1 homoeologs by CRISPR/Cas9-mediated genome editing.The triple mutants displayed lower FSN,GNS,grain number per spikelet(GNST),and TGW than wild-type plants.In 306 hexaploid wheat accessions,two single-nucleotide polymorphisms in TaSus-A1 contributed differently to GNS.Introgression of the two alleles into a wheat genetic background confirmed their effects.The alleles differed in geographical distribution among the accessions.展开更多
The additives such as phosphoric acid, calcium phosphate, calcium super phosphate, calcium over super phosphate, calcium carbonate, sodium hydrosulphite, etc. were used to produce furfural from the straw by hydrolys...The additives such as phosphoric acid, calcium phosphate, calcium super phosphate, calcium over super phosphate, calcium carbonate, sodium hydrosulphite, etc. were used to produce furfural from the straw by hydrolysis with sulfuric acid. The effect of amount of the additives, the content of the added substance and the conditions of distillation on the acidity of the residues were studied. The experiment results showed that the all residues became neutral complex fertilizer, and the productivity of furfural increases under the following conditions: sulfuric acid concentration is 20% (by weight), the ratio of liquid to solid is 3∶1—4∶1 (by weight), the ratio of the additives to straw is suitable.展开更多
基金This work was supported by the Strategic Priority Research Program of Chinese Academy of Sciences(XDA24010104-2).
文摘Some haplotypes of the sucrose synthase gene TaSus1 are associated with thousand-grain weight(TGW)in wheat(Triticum aestivum L.).However,no mutations have been identified within the gene to test this association.The effects of TaSus1 on grain number per spike(GNS)also are largely unknown.Our previous genome-wide association study identified TaSus-A1 as a candidate gene controlling fertile spikelet number per spike(FSN).In the present study,we generated two independent mutants for the three TaSus1 homoeologs by CRISPR/Cas9-mediated genome editing.The triple mutants displayed lower FSN,GNS,grain number per spikelet(GNST),and TGW than wild-type plants.In 306 hexaploid wheat accessions,two single-nucleotide polymorphisms in TaSus-A1 contributed differently to GNS.Introgression of the two alleles into a wheat genetic background confirmed their effects.The alleles differed in geographical distribution among the accessions.
文摘The additives such as phosphoric acid, calcium phosphate, calcium super phosphate, calcium over super phosphate, calcium carbonate, sodium hydrosulphite, etc. were used to produce furfural from the straw by hydrolysis with sulfuric acid. The effect of amount of the additives, the content of the added substance and the conditions of distillation on the acidity of the residues were studied. The experiment results showed that the all residues became neutral complex fertilizer, and the productivity of furfural increases under the following conditions: sulfuric acid concentration is 20% (by weight), the ratio of liquid to solid is 3∶1—4∶1 (by weight), the ratio of the additives to straw is suitable.
