[Objectives]To analyze the composition of lycophytes and ferns in Yunzhongshan Nature Reserve,Fujian Province.[Methods]This study examined the composition of lycophytes and ferns in Yunzhongshan Nature Reserve through...[Objectives]To analyze the composition of lycophytes and ferns in Yunzhongshan Nature Reserve,Fujian Province.[Methods]This study examined the composition of lycophytes and ferns in Yunzhongshan Nature Reserve through field investigations and specimen identification,supplemented by a review of relevant literature.[Results]A total of 84 species of lycophytes and ferns were identified,encompassing 55 genera and 29 families.This included 14 species of lycophytes distributed across 8 genera and 3 families,as well as 70 species of ferns belonging to 47 genera and 26 families.Polypodiaceae and Selaginella were the dominant family and genus,respectively,while oligospecific families and monotypic genera were significant components of lycophytes and ferns in the region.The distribution types of these families were primarily cosmopolitan and tropical.The Germline differentiation degree(SD)was 3.34,and the proportion of rare or endangered species among lycophytes and ferns was 8.33%.[Conclusions]Yunzhongshan Nature Reserve possesses a diverse array of lycophyte and fern species.This study offers valuable scientific insights that can inform efforts related to the conservation of biodiversity,resource utilization and development,species introduction,and system evolution of lycophytes and ferns within the reserve.展开更多
The Indo-Burma Biodiversity Hotspot is renowned for its rich biodiversity,including that of vascular plants.However,the fern diversity and its endemism in this hotspot have not been well understood and so far,the dive...The Indo-Burma Biodiversity Hotspot is renowned for its rich biodiversity,including that of vascular plants.However,the fern diversity and its endemism in this hotspot have not been well understood and so far,the diversity of very few groups of ferns in this region has been explored using combined molecular and morphological approaches.Here,we updated the plastid phylogeny of the Java fern genus Leptochilus with 226(115%increase of the latest sampling)samples across the distribution range,specifically those of three phylogenetically significant species,Leptochilus ovatus,L.pedunculatus,and L.pothifolius.We also reconstructed the first nuclear phylogeny of the genus based on pgiC gene data.Based on molecular and morphological evidence,we identified three new major clades and six new subclades,redefined three existing species,discovered a number of cryptic species of the genus,and elucidated the evolution of the three most variable characters.Our divergence time analyses and ancestral area reconstruction showed that Leptochilus originated in the Oligocene and diversified from early Miocene and 15 dispersal events from lower to higher latitudes are identified.The evolution of three most important morphological characters is analyzed in a context of the new phylogeny.Our analysis showed that 30(59%of total 51)species of Leptochilus occur in Indo-Burma hotspot,24(80%of the 30 species)of which are endemic to this hotspot.We argue that the Indo-Burma hotspot should be recognized as a diversity hotspot for ferns.展开更多
Through investigating the wild ornamental ferns in Guizhou Province, it is found that there are totally 312 species (including variations), 111 genera and 49 families of such resources in this region, which are classi...Through investigating the wild ornamental ferns in Guizhou Province, it is found that there are totally 312 species (including variations), 111 genera and 49 families of such resources in this region, which are classified into terrestrial fern, epiphytic fern, saxicolous fern, vine fern, tree fern and aquatic fern according to their ecological types. The application of ornamental ferns in garden landscaping and development countermeasures are analyzed in this study.展开更多
Suitable habitat is vital for the survival and restoration of a species.Understanding the suitable habitat range for lycophytes and ferns is prerequisite for effective species resource conservation and recovery effort...Suitable habitat is vital for the survival and restoration of a species.Understanding the suitable habitat range for lycophytes and ferns is prerequisite for effective species resource conservation and recovery efforts.In this study, we took Athyrium brevifrons as an example, predicted its suitable habitat using a Maxent model with 67 occurrence data and nine environmental variables in Northeast China.The area under the curve(AUC) value of independent test data, as well as the comparison with specimen county areal distribution of A.brevifrons exhibited excellent predictive performance.The type of environmental variables showed that precipitation contributed the most to the distribution prediction, followed by temperature and topography.Percentage contribution and permutation importance both indicated that precipitation of driest quarter(Bio17) was the key factor in determining the natural distribution of A.brevifrons, the reason could be proved by the fern gametophyte biology.The analysis of high habitat suitability areas also showed the habitat preference of A.brevifrons: comparatively more precipitation and less fluctuation in the driest quarter.Changbai Mountains, covering almost all the high and medium habitat suitability areas, provide the best ecological conditions for the survival of A.brevifrons, and should be considered as priority areas for protection and restoration of the wild resource.The potential habitat suitability distribution map could provide a reference for the sustainable development and utilisation of A.brevifrons resource, and Maxent modelling could be valuable for conservation management planning for lycophytes and ferns in Northeast China.展开更多
Ferns and lycophytes have remarkably large genomes.However,little is known about how their genome size evolved in fern lineages.To explore the origins and evolution of chromosome numbers and genome size in ferns,we us...Ferns and lycophytes have remarkably large genomes.However,little is known about how their genome size evolved in fern lineages.To explore the origins and evolution of chromosome numbers and genome size in ferns,we used flow cytometry to measure the genomes of 240 species(255 samples)of extant ferns and lycophytes comprising 27 families and 72 genera,of which 228 species(242 samples)represent new reports.We analyzed correlations among genome size,spore size,chromosomal features,phylogeny,and habitat type preference within a phylogenetic framework.We also applied ANOVA and multinomial logistic regression analysis to preference of habitat type and genome size.Using the phylogeny,we conducted ancestral character reconstruction for habitat types and tested whether genome size changes simultaneously with shifts in habitat preference.We found that 2 C values had weak phylogenetic signal,whereas the base number of chromosomes(x)had a strong phylogenetic signal.Furthermore,our analyses revealed a positive correlation between genome size and chromosome traits,indicating that the base number of chromosomes(x),chromosome size,and polyploidization may be primary contributors to genome expansion in ferns and lycophytes.Genome sizes in different habitat types varied significantly and were significantly correlated with habitat types;specifically,multinomial logistic regression indicated that species with larger 2 C values were more likely to be epiphytes.Terrestrial habitat is inferred to be ancestral for both extant ferns and lycophytes,whereas transitions to other habitat types occurred as the major clades emerged.Shifts in habitat types appear be followed by periods of genomic stability.Based on these results,we inferred that habitat type changes and multiple whole-genome duplications have contributed to the formation of large genomes of ferns and their allies during their evolutionary history.展开更多
We analyzed the distributional patterns of95 selected species of leptosporangiate ferns inhabiting the Mexican Mountain Component, using grid-cells of one geographical degree as unit areas,applying endemism indices an...We analyzed the distributional patterns of95 selected species of leptosporangiate ferns inhabiting the Mexican Mountain Component, using grid-cells of one geographical degree as unit areas,applying endemism indices and richness and beta diversity analyses.Distributional data were obtained from several herbaria and specialized literature.Five grid-cells appear to be important for fern species richness, as they contain 35 to 49 species.These gridcells are located in the Sierra Madre Oriental(SMO),Trans-Mexican Volcanic Belt(TMVB) and the Sierra Madre del Sur(SMS).Mean richness by latitudinal belts of one degree showed that the belts with highest values are related to the TMVB and SMS.A total of 13grid-cells were recognized as important from theperspective of endemism; most of them are located also in the SMO, TMVB and SMS.The richest gridcells coincided with one of the main centres of endemism for ferns obtained in this study, located in the convergence of the southern part of the SMO, the eastern portion of the TMVB and the northern part of the SMS, reflecting the high humidity existing on the mountain slopes facing the Gulf of Mexico.Some important grid-cells recognized from richness and endemism analyses coincide with Mexican Natural Protected Areas.The beta diversity analysis showed a low degree of similarity among grid-cells, implying a high species replacement, as the result of environmental heterogeneity occurring in the Mexican mountain systems.On the other hand, the spatial analysis suggested a pattern of phytogeographical regionalization comprising twomain areas: the Mexican Transition Zone and the Mexican Central Plateau.Ferns play an important role in the Mexican biodiversity and contributing to the beta diversity of Mexico.展开更多
Lycophytes and ferns are unique and charismatic members of many terrestrial ecosystems and occupy the pivotal position in land plant origin and evolution.The Chinese lycophytes and ferns flora,with approximately 2000 ...Lycophytes and ferns are unique and charismatic members of many terrestrial ecosystems and occupy the pivotal position in land plant origin and evolution.The Chinese lycophytes and ferns flora,with approximately 2000 species,contributes a substantial component to the global lycophytes and ferns diversity,with estimates of 12000 species.Among them,about 433 species are medicinally recorded and researches based on their phytochemical properties are important topics in natural medicines.This paper reviewed the research history and current status of chemical constituents and biological activities of lycophytes and ferns,which had highlighted the research progress of our group.展开更多
64 species （including 5 varieties）, 30 genera and 24 families of the edible ferns in Guizhou are preliminartiy confirmed, according to the combination of spot investigation and refereuce analySis：the species, gener...64 species （including 5 varieties）, 30 genera and 24 families of the edible ferns in Guizhou are preliminartiy confirmed, according to the combination of spot investigation and refereuce analySis：the species, genera and families account for 8.13%, 19.74% and 44.44% of ferns in Guizhou, respectively. The floristic composition characters and resource distribution of 23 kinds of the edible ferns in Guizhou are described.展开更多
Background Transgenic research in crops involves using genetic engineering techniques to introduce specific genes of interest from other organisms,or even entirely new genes into plant genomes to create crops with des...Background Transgenic research in crops involves using genetic engineering techniques to introduce specific genes of interest from other organisms,or even entirely new genes into plant genomes to create crops with desirable traits that wouldn’t be possible through conventional breeding methods.Transgenic crops have been developed for various traits globally.Whitefly,Bemisia tabaci(Gennadius)is one of the major sucking pests of cotton that cause significant damage to the cotton production.To combat whitefly infestations,researchers have developed four transgenic cotton lines expressing the fern protein.And those transgenic lines need to be evaluated for their performance against the target pest—whitefly.The evaluation was designed as controlled trials in polyhouse or muslin cloth cages under open-choice and no-choice conditions by comparing four transgenic cotton lines(A,B,C,and D)with three control groups,including untransformed cotton plants with a same genetic background of the transgenic line,conventionally bred whitefly-resistant cotton,and whitefly-susceptible cotton.In order to study the generational effect,the evaluation also involved studies on whitefly development in laboratory,muslin cloth cage,and polyhouse conditions.Results Both open-choice and no-choice experiments had shown that all the four transgenic cotton lines(A,B,C,and D)expressing the fern protein reduced adult whitefly numbers significantly compared with the control lines,except for the no-choice conditions in 2021,where the transgenic line C was non-significant different from the resistant control line.Notably,the nymphal population on the resistant control line was relatively low and nonsignificant different from the transgenic line C in 2021;and the transgenic lines A and C in 2022 under open-choice conditions.Under no-choice condition,the nymphal counts in the resistant control line was non-significant different from transgenic lines C and D in 2021;and transgenic line D in 2022.All transgenic lines showed significant decrease in egg hatching in 2021 and nymphal development in 2022,except for the transgenic line C which had no significant different in the nymphal development comparing with non-transgenic control lines in 2022.Adult emergence rates in both years of evaluation showed significant decrease in transgenic lines A and B comparing with the control lines.Additionally,the results showed a significant reduction in cotton leaf curl disease and sooty mold development in all the four transgenic lines compared with susceptible control under open-choice conditions,indicating potential benefits of transgenic lines beyond direct effect on whitefly control.Furthermore,the research explored the generational effects of the fern protein on whitefly which revealed the lowest fecundity in the transgenic line C across F0,F1 and F3 generations,lower egg hatching in F1 and F2 generations in transgenic lines A and B,shorter nymphal duration in F1 and F2 generations in transgenic line B,and the least total adult emergence in the transgenic line C in F0 and F3 generations.Conclusions These findings suggest that the transgenic cotton lines expressing fern protein disrupts whitefly populations and the life cycle to a certain extent.However,results are not consistent over generations and years of study,indicating these transgenic lines were not superior over control lines and need to be improved in future breeding.展开更多
The Chilean Pampean flat slab subduction segment is characterized by the nearly horizontal subduction of the Nazca Plate within the depth range of 100-120 km.Numerous seismic tomography studies have been conducted to ...The Chilean Pampean flat slab subduction segment is characterized by the nearly horizontal subduction of the Nazca Plate within the depth range of 100-120 km.Numerous seismic tomography studies have been conducted to investigate its velocity structure;however,they have used only seismic body wave data or surface wave data.As a result,the existing velocity models in the region may have relatively large uncertainties.In this study,we use body wave arrival times from earthquakes occurring in central Chile between 2014 and 2019,as well as Rayleigh wave phase velocity maps at periods of 5-80 s from ambient noise empirical Green’s functions in Chile.By jointly using body wave arrival times and surface wave dispersion data,we refine the VS model and improve earthquake locations in the central Chile subduction zone.Compared with previous velocity models,our velocity model better reveals an eastward-dipping high-velocity plate representing the subducting Nazca Plate,which is 40-50 km thick and is more consistent with the slab thickness estimated by receiver function imaging and thermal modeling.Overall,the intraslab seismicity distribution spatially correlates well with the slab high-velocity anomalies except along the subduction paths of the CopiapóRidge and Juan Fernández Ridge.Additionally,parallel low-velocity stripes are imaged beneath the subducting plate,which are likely associated with the accumulated melts.The joint inversion velocity model also resolves widespread low-velocity anomalies in the crust beneath the Central Volcanic Zone of the central Andes,likely representing crustal magma chambers for various volcanoes.展开更多
基金Supported by National Key Protected Plant Investigation Project in Anxi Yunzhongshan Nature Reserve Primarily Focusing on Alsophila spinulosa,Paris polyphylla,and Angiopteris fokiensis([350524]BWZ[CS]2024003).
文摘[Objectives]To analyze the composition of lycophytes and ferns in Yunzhongshan Nature Reserve,Fujian Province.[Methods]This study examined the composition of lycophytes and ferns in Yunzhongshan Nature Reserve through field investigations and specimen identification,supplemented by a review of relevant literature.[Results]A total of 84 species of lycophytes and ferns were identified,encompassing 55 genera and 29 families.This included 14 species of lycophytes distributed across 8 genera and 3 families,as well as 70 species of ferns belonging to 47 genera and 26 families.Polypodiaceae and Selaginella were the dominant family and genus,respectively,while oligospecific families and monotypic genera were significant components of lycophytes and ferns in the region.The distribution types of these families were primarily cosmopolitan and tropical.The Germline differentiation degree(SD)was 3.34,and the proportion of rare or endangered species among lycophytes and ferns was 8.33%.[Conclusions]Yunzhongshan Nature Reserve possesses a diverse array of lycophyte and fern species.This study offers valuable scientific insights that can inform efforts related to the conservation of biodiversity,resource utilization and development,species introduction,and system evolution of lycophytes and ferns within the reserve.
基金supported by the Second Tibetan Plateau Scientific Expedition and Research(STEP)program(2019QZKK0502)a grant from Yunnan Fundamental Research Projects(Grant#202201BC070001)a Yunnan Revitalization Talent Support Program“Young Talent”Project and a CAS Scholarship to Liang Zhang.
文摘The Indo-Burma Biodiversity Hotspot is renowned for its rich biodiversity,including that of vascular plants.However,the fern diversity and its endemism in this hotspot have not been well understood and so far,the diversity of very few groups of ferns in this region has been explored using combined molecular and morphological approaches.Here,we updated the plastid phylogeny of the Java fern genus Leptochilus with 226(115%increase of the latest sampling)samples across the distribution range,specifically those of three phylogenetically significant species,Leptochilus ovatus,L.pedunculatus,and L.pothifolius.We also reconstructed the first nuclear phylogeny of the genus based on pgiC gene data.Based on molecular and morphological evidence,we identified three new major clades and six new subclades,redefined three existing species,discovered a number of cryptic species of the genus,and elucidated the evolution of the three most variable characters.Our divergence time analyses and ancestral area reconstruction showed that Leptochilus originated in the Oligocene and diversified from early Miocene and 15 dispersal events from lower to higher latitudes are identified.The evolution of three most important morphological characters is analyzed in a context of the new phylogeny.Our analysis showed that 30(59%of total 51)species of Leptochilus occur in Indo-Burma hotspot,24(80%of the 30 species)of which are endemic to this hotspot.We argue that the Indo-Burma hotspot should be recognized as a diversity hotspot for ferns.
文摘Through investigating the wild ornamental ferns in Guizhou Province, it is found that there are totally 312 species (including variations), 111 genera and 49 families of such resources in this region, which are classified into terrestrial fern, epiphytic fern, saxicolous fern, vine fern, tree fern and aquatic fern according to their ecological types. The application of ornamental ferns in garden landscaping and development countermeasures are analyzed in this study.
基金Under the auspices of National Key Research and Development Program of China(No.2016YFC0500300)National Natural Science Foundation of China(No.41675153)
文摘Suitable habitat is vital for the survival and restoration of a species.Understanding the suitable habitat range for lycophytes and ferns is prerequisite for effective species resource conservation and recovery efforts.In this study, we took Athyrium brevifrons as an example, predicted its suitable habitat using a Maxent model with 67 occurrence data and nine environmental variables in Northeast China.The area under the curve(AUC) value of independent test data, as well as the comparison with specimen county areal distribution of A.brevifrons exhibited excellent predictive performance.The type of environmental variables showed that precipitation contributed the most to the distribution prediction, followed by temperature and topography.Percentage contribution and permutation importance both indicated that precipitation of driest quarter(Bio17) was the key factor in determining the natural distribution of A.brevifrons, the reason could be proved by the fern gametophyte biology.The analysis of high habitat suitability areas also showed the habitat preference of A.brevifrons: comparatively more precipitation and less fluctuation in the driest quarter.Changbai Mountains, covering almost all the high and medium habitat suitability areas, provide the best ecological conditions for the survival of A.brevifrons, and should be considered as priority areas for protection and restoration of the wild resource.The potential habitat suitability distribution map could provide a reference for the sustainable development and utilisation of A.brevifrons resource, and Maxent modelling could be valuable for conservation management planning for lycophytes and ferns in Northeast China.
基金the National Natural Science Foundation of China(grant number 31870188,31800174,31700172,41571056)to Wang,Shen,Wang and XingShanghai Landscaping and City Appearance Administrative Bureau of China,Scientific Research Grants(G182411)to Yan+1 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(grant number XDA13020603,XDA13020500)to Chen and JianGuangdong Natural Science Foundation(grant number 2015A030308015)to Wang。
文摘Ferns and lycophytes have remarkably large genomes.However,little is known about how their genome size evolved in fern lineages.To explore the origins and evolution of chromosome numbers and genome size in ferns,we used flow cytometry to measure the genomes of 240 species(255 samples)of extant ferns and lycophytes comprising 27 families and 72 genera,of which 228 species(242 samples)represent new reports.We analyzed correlations among genome size,spore size,chromosomal features,phylogeny,and habitat type preference within a phylogenetic framework.We also applied ANOVA and multinomial logistic regression analysis to preference of habitat type and genome size.Using the phylogeny,we conducted ancestral character reconstruction for habitat types and tested whether genome size changes simultaneously with shifts in habitat preference.We found that 2 C values had weak phylogenetic signal,whereas the base number of chromosomes(x)had a strong phylogenetic signal.Furthermore,our analyses revealed a positive correlation between genome size and chromosome traits,indicating that the base number of chromosomes(x),chromosome size,and polyploidization may be primary contributors to genome expansion in ferns and lycophytes.Genome sizes in different habitat types varied significantly and were significantly correlated with habitat types;specifically,multinomial logistic regression indicated that species with larger 2 C values were more likely to be epiphytes.Terrestrial habitat is inferred to be ancestral for both extant ferns and lycophytes,whereas transitions to other habitat types occurred as the major clades emerged.Shifts in habitat types appear be followed by periods of genomic stability.Based on these results,we inferred that habitat type changes and multiple whole-genome duplications have contributed to the formation of large genomes of ferns and their allies during their evolutionary history.
基金Support from projects PAPIIT IN215914 and IV201015
文摘We analyzed the distributional patterns of95 selected species of leptosporangiate ferns inhabiting the Mexican Mountain Component, using grid-cells of one geographical degree as unit areas,applying endemism indices and richness and beta diversity analyses.Distributional data were obtained from several herbaria and specialized literature.Five grid-cells appear to be important for fern species richness, as they contain 35 to 49 species.These gridcells are located in the Sierra Madre Oriental(SMO),Trans-Mexican Volcanic Belt(TMVB) and the Sierra Madre del Sur(SMS).Mean richness by latitudinal belts of one degree showed that the belts with highest values are related to the TMVB and SMS.A total of 13grid-cells were recognized as important from theperspective of endemism; most of them are located also in the SMO, TMVB and SMS.The richest gridcells coincided with one of the main centres of endemism for ferns obtained in this study, located in the convergence of the southern part of the SMO, the eastern portion of the TMVB and the northern part of the SMS, reflecting the high humidity existing on the mountain slopes facing the Gulf of Mexico.Some important grid-cells recognized from richness and endemism analyses coincide with Mexican Natural Protected Areas.The beta diversity analysis showed a low degree of similarity among grid-cells, implying a high species replacement, as the result of environmental heterogeneity occurring in the Mexican mountain systems.On the other hand, the spatial analysis suggested a pattern of phytogeographical regionalization comprising twomain areas: the Mexican Transition Zone and the Mexican Central Plateau.Ferns play an important role in the Mexican biodiversity and contributing to the beta diversity of Mexico.
基金supported by the National Natural Science Foundation of China(No.21837003)Open fund from the State Key Laboratory of Phytochemistry and Plant Resources in West China(No.P2017-KF13)
文摘Lycophytes and ferns are unique and charismatic members of many terrestrial ecosystems and occupy the pivotal position in land plant origin and evolution.The Chinese lycophytes and ferns flora,with approximately 2000 species,contributes a substantial component to the global lycophytes and ferns diversity,with estimates of 12000 species.Among them,about 433 species are medicinally recorded and researches based on their phytochemical properties are important topics in natural medicines.This paper reviewed the research history and current status of chemical constituents and biological activities of lycophytes and ferns,which had highlighted the research progress of our group.
基金Supported by Guiyang Science and Technology Planning Project~~
文摘64 species （including 5 varieties）, 30 genera and 24 families of the edible ferns in Guizhou are preliminartiy confirmed, according to the combination of spot investigation and refereuce analySis：the species, genera and families account for 8.13%, 19.74% and 44.44% of ferns in Guizhou, respectively. The floristic composition characters and resource distribution of 23 kinds of the edible ferns in Guizhou are described.
文摘Background Transgenic research in crops involves using genetic engineering techniques to introduce specific genes of interest from other organisms,or even entirely new genes into plant genomes to create crops with desirable traits that wouldn’t be possible through conventional breeding methods.Transgenic crops have been developed for various traits globally.Whitefly,Bemisia tabaci(Gennadius)is one of the major sucking pests of cotton that cause significant damage to the cotton production.To combat whitefly infestations,researchers have developed four transgenic cotton lines expressing the fern protein.And those transgenic lines need to be evaluated for their performance against the target pest—whitefly.The evaluation was designed as controlled trials in polyhouse or muslin cloth cages under open-choice and no-choice conditions by comparing four transgenic cotton lines(A,B,C,and D)with three control groups,including untransformed cotton plants with a same genetic background of the transgenic line,conventionally bred whitefly-resistant cotton,and whitefly-susceptible cotton.In order to study the generational effect,the evaluation also involved studies on whitefly development in laboratory,muslin cloth cage,and polyhouse conditions.Results Both open-choice and no-choice experiments had shown that all the four transgenic cotton lines(A,B,C,and D)expressing the fern protein reduced adult whitefly numbers significantly compared with the control lines,except for the no-choice conditions in 2021,where the transgenic line C was non-significant different from the resistant control line.Notably,the nymphal population on the resistant control line was relatively low and nonsignificant different from the transgenic line C in 2021;and the transgenic lines A and C in 2022 under open-choice conditions.Under no-choice condition,the nymphal counts in the resistant control line was non-significant different from transgenic lines C and D in 2021;and transgenic line D in 2022.All transgenic lines showed significant decrease in egg hatching in 2021 and nymphal development in 2022,except for the transgenic line C which had no significant different in the nymphal development comparing with non-transgenic control lines in 2022.Adult emergence rates in both years of evaluation showed significant decrease in transgenic lines A and B comparing with the control lines.Additionally,the results showed a significant reduction in cotton leaf curl disease and sooty mold development in all the four transgenic lines compared with susceptible control under open-choice conditions,indicating potential benefits of transgenic lines beyond direct effect on whitefly control.Furthermore,the research explored the generational effects of the fern protein on whitefly which revealed the lowest fecundity in the transgenic line C across F0,F1 and F3 generations,lower egg hatching in F1 and F2 generations in transgenic lines A and B,shorter nymphal duration in F1 and F2 generations in transgenic line B,and the least total adult emergence in the transgenic line C in F0 and F3 generations.Conclusions These findings suggest that the transgenic cotton lines expressing fern protein disrupts whitefly populations and the life cycle to a certain extent.However,results are not consistent over generations and years of study,indicating these transgenic lines were not superior over control lines and need to be improved in future breeding.
基金funded by the National Natural Science Foundation of China under Grant 42230101the National Agency for Research and Development of Chile(ANID)by Projects AFB180004 and AFB220002the ANID Programa de Cooperación Internacional(PCI)Grant PII-180003.
文摘The Chilean Pampean flat slab subduction segment is characterized by the nearly horizontal subduction of the Nazca Plate within the depth range of 100-120 km.Numerous seismic tomography studies have been conducted to investigate its velocity structure;however,they have used only seismic body wave data or surface wave data.As a result,the existing velocity models in the region may have relatively large uncertainties.In this study,we use body wave arrival times from earthquakes occurring in central Chile between 2014 and 2019,as well as Rayleigh wave phase velocity maps at periods of 5-80 s from ambient noise empirical Green’s functions in Chile.By jointly using body wave arrival times and surface wave dispersion data,we refine the VS model and improve earthquake locations in the central Chile subduction zone.Compared with previous velocity models,our velocity model better reveals an eastward-dipping high-velocity plate representing the subducting Nazca Plate,which is 40-50 km thick and is more consistent with the slab thickness estimated by receiver function imaging and thermal modeling.Overall,the intraslab seismicity distribution spatially correlates well with the slab high-velocity anomalies except along the subduction paths of the CopiapóRidge and Juan Fernández Ridge.Additionally,parallel low-velocity stripes are imaged beneath the subducting plate,which are likely associated with the accumulated melts.The joint inversion velocity model also resolves widespread low-velocity anomalies in the crust beneath the Central Volcanic Zone of the central Andes,likely representing crustal magma chambers for various volcanoes.
