Objective A subcutaneous transplantation tumor model of human HT-29 cells was established in nude mice to study the anticarcinogenic activities and apoptosis-regulatory mechanistic effect of aqueous extract of ferment...Objective A subcutaneous transplantation tumor model of human HT-29 cells was established in nude mice to study the anticarcinogenic activities and apoptosis-regulatory mechanistic effect of aqueous extract of fermented barley with Lactobacillus plantarum dy-1 （LFBE）. Methods HT-29 cells were transplanted via subcutaneous injection of 1 × 107cells into the right flank of each nude mouse. Then, nude mice were treated for 30 days with LFBE （high-dose 2 g·kg-1·d-1; low-dose 1 g·kg-1·d-1） and for 7 days with 5-fluorouracil （5-FU, 25 g·kg-1·d-1） by gavage and intraperitoneal injection, respectively. Results Tumor volume and weight decreased significantly in both groups of nude mice treated with LFBE. In addition, the cell apoptosis rate of the LFBE group was significantly higher than that of the control group and 5-FU groups as measured by the TUNEL assay. Moreover, the real-time fluorescent quantitative PCR and Western blot methods further confirmed these apoptosis-enhancing and growth-inhibiting effects. The involvement of LFBE in inducing apoptosis was confirmed by the expression of Bax, Bcl-2, caspase-3, and cyclin D1. Conclusion The results showed that LFBE could induce subcutaneous transplantation tumor apoptosis in nude mice and could be used as a natural nutrient supplement or chemopreventive agent in the treatment of human colon cancer.展开更多
Objective To investigate the effect of fermented barley extracts with Lactobacillus plantarum dy-1（LFBE） for modulating glucose consumption in HepG2 cells via miR-212 regulation. Methods Hepatocellular carcinoma（He...Objective To investigate the effect of fermented barley extracts with Lactobacillus plantarum dy-1（LFBE） for modulating glucose consumption in HepG2 cells via miR-212 regulation. Methods Hepatocellular carcinoma（HepG2） cells were treated with palmitate. After 12 h, palmitate-induced HepG2 cells were treated with LFBE and its main components. Changes in glucose consumption, proinflammatory cytokine secretion, and miRNA-212 expression in HepG2 cells was observed. Results Treatment with LFBE rich in vanillic acid（VA） increased glucose consumption and reduced proinflammatory cytokine secretion in HepG2 cells. LFBE and VA normalized the upregulation of miR-212, which led to the upregulation of dual-specificity phosphatase-9（DUSP9）, a direct target of miR-212, at both protein and mR NA levels. Downregulation of miR-212 markedly increased glucose consumption and reduced proinflammatory cytokine secretion by enhancing DUSP9 expression. Conclusion The results showed the benefit of LFBE and miR-212 downregulation in modulating glucose consumption and reducing proinflammatory cytokine secretion by targeting DUSP9. VA in LFBE was a strong regulator of palmitate-induced abnormal glucose consumption in HepG2 cells and can be a primary mediator.展开更多
The present study was aimed that determine the effects of polysaccharide isolated from fermented barley about tumor metastasis inhibition via immune related factors such as complementary system,macrophages,natural kil...The present study was aimed that determine the effects of polysaccharide isolated from fermented barley about tumor metastasis inhibition via immune related factors such as complementary system,macrophages,natural killer(NK)cells,and cytotoxic T lymphocyte(CTL).To characterize the tumor inhibition effects through immunostimulation of polysaccharides purified from fermented barley,the active crude polysaccharide(BF-CP)was isolated from fermented barley and further fractionated into three purified polysaccharides(BF-I,-II,and-III)with different molecular weights through gel permeation chromatography.BF-I was mainly composed of four monosaccharides,including xylose(32.90%),arabinose(26.71%),and glucose(33.43%).BF-I augmented the secretion of cytokines,such as interleukin(IL)-6,IL-12,and tumor necrosis factor-α,through peritoneal macrophages and potently activated the complement system.In the experiment for NK cells and CTL activities,intravenous(i.v.)administration of BF-I significantly augmented the cytotoxicity of NK cells and CTL-mediated cytotoxicity against tumor cells.In addition,i.v.administration of BF-I potently inhibited tumor metastasis in a mouse model of lung cancer and tumor metastasis in mice with suppressed NK cell function through anti-asialo GM1.Consequently,BF-I shows potent tumor inhibition effects,and these effects are related to various immune factors such as macrophages,the complement system,NK cells,and CTL.Therefore,BF-I,the polysaccharide purified from fermented barley possess the new possibility for developing functional foods and medical ingredients.展开更多
基金supported by the priority academic program development of Jiangsu higher education institutionsthe graduate research and innovation projects of Jiangsu province(CXZZ13_0694)
文摘Objective A subcutaneous transplantation tumor model of human HT-29 cells was established in nude mice to study the anticarcinogenic activities and apoptosis-regulatory mechanistic effect of aqueous extract of fermented barley with Lactobacillus plantarum dy-1 （LFBE）. Methods HT-29 cells were transplanted via subcutaneous injection of 1 × 107cells into the right flank of each nude mouse. Then, nude mice were treated for 30 days with LFBE （high-dose 2 g·kg-1·d-1; low-dose 1 g·kg-1·d-1） and for 7 days with 5-fluorouracil （5-FU, 25 g·kg-1·d-1） by gavage and intraperitoneal injection, respectively. Results Tumor volume and weight decreased significantly in both groups of nude mice treated with LFBE. In addition, the cell apoptosis rate of the LFBE group was significantly higher than that of the control group and 5-FU groups as measured by the TUNEL assay. Moreover, the real-time fluorescent quantitative PCR and Western blot methods further confirmed these apoptosis-enhancing and growth-inhibiting effects. The involvement of LFBE in inducing apoptosis was confirmed by the expression of Bax, Bcl-2, caspase-3, and cyclin D1. Conclusion The results showed that LFBE could induce subcutaneous transplantation tumor apoptosis in nude mice and could be used as a natural nutrient supplement or chemopreventive agent in the treatment of human colon cancer.
基金supported by the priority academic program development of Jiangsu Higher education institutions and the National Natural Science Foundation of China [3150156931371760]
文摘Objective To investigate the effect of fermented barley extracts with Lactobacillus plantarum dy-1（LFBE） for modulating glucose consumption in HepG2 cells via miR-212 regulation. Methods Hepatocellular carcinoma（HepG2） cells were treated with palmitate. After 12 h, palmitate-induced HepG2 cells were treated with LFBE and its main components. Changes in glucose consumption, proinflammatory cytokine secretion, and miRNA-212 expression in HepG2 cells was observed. Results Treatment with LFBE rich in vanillic acid（VA） increased glucose consumption and reduced proinflammatory cytokine secretion in HepG2 cells. LFBE and VA normalized the upregulation of miR-212, which led to the upregulation of dual-specificity phosphatase-9（DUSP9）, a direct target of miR-212, at both protein and mR NA levels. Downregulation of miR-212 markedly increased glucose consumption and reduced proinflammatory cytokine secretion by enhancing DUSP9 expression. Conclusion The results showed the benefit of LFBE and miR-212 downregulation in modulating glucose consumption and reducing proinflammatory cytokine secretion by targeting DUSP9. VA in LFBE was a strong regulator of palmitate-induced abnormal glucose consumption in HepG2 cells and can be a primary mediator.
基金supported by Kyonggi University’s Graduate Research Assistantship 2022.
文摘The present study was aimed that determine the effects of polysaccharide isolated from fermented barley about tumor metastasis inhibition via immune related factors such as complementary system,macrophages,natural killer(NK)cells,and cytotoxic T lymphocyte(CTL).To characterize the tumor inhibition effects through immunostimulation of polysaccharides purified from fermented barley,the active crude polysaccharide(BF-CP)was isolated from fermented barley and further fractionated into three purified polysaccharides(BF-I,-II,and-III)with different molecular weights through gel permeation chromatography.BF-I was mainly composed of four monosaccharides,including xylose(32.90%),arabinose(26.71%),and glucose(33.43%).BF-I augmented the secretion of cytokines,such as interleukin(IL)-6,IL-12,and tumor necrosis factor-α,through peritoneal macrophages and potently activated the complement system.In the experiment for NK cells and CTL activities,intravenous(i.v.)administration of BF-I significantly augmented the cytotoxicity of NK cells and CTL-mediated cytotoxicity against tumor cells.In addition,i.v.administration of BF-I potently inhibited tumor metastasis in a mouse model of lung cancer and tumor metastasis in mice with suppressed NK cell function through anti-asialo GM1.Consequently,BF-I shows potent tumor inhibition effects,and these effects are related to various immune factors such as macrophages,the complement system,NK cells,and CTL.Therefore,BF-I,the polysaccharide purified from fermented barley possess the new possibility for developing functional foods and medical ingredients.
