The safety of crop protection products and their safe use is of paramount importance and requires that crop protection products are consistently marketed and used worldwide in the required registered quality.To meet t...The safety of crop protection products and their safe use is of paramount importance and requires that crop protection products are consistently marketed and used worldwide in the required registered quality.To meet these high-quality requirements,the highest demands must be placed on the production and purification of technical-grade crop protection products.In this context,impurity profiles are of utmost importance,as unknown impurities at higher than registered concentrations may pose new unknown toxicological risks to users,consumers,and the environment.Particularly high demands are placed on the production and purification of technical grade semisynthetic derivatives of fermentatively derived natural products to meet the required regulatory specifications,to ensure that relevant impurities do not exceed maximum allowable concentrations,to ensure the safe use of such products,and to eliminate any unknown product safety risks.The challenges associated with the manufacture and purification of Afidopyropen and the product safety risks associated with various qualities of technical grade Emamectin benzoate are discussed in detail.展开更多
Early life is a critical period for metabolic and immune development,during which nutritional inputs may in-fluence physiological maturation beyond basic nutrient supply.This study evaluated a milk-based nutritional f...Early life is a critical period for metabolic and immune development,during which nutritional inputs may in-fluence physiological maturation beyond basic nutrient supply.This study evaluated a milk-based nutritional formulation as an early-life intervention in lambs.The study further examined associations with microbial fermentation,systemic metabolism,immune responses,growth performance,and maternal physiological re-sponses.Synthetic milk supplementation resulted in body weight gain and average daily gain in lambs,together with elevated circulating growth hormone and insulin-like growth factor-1 levels.Immune indicators,including IgA and IgM,were increased,while pro-inflammatory cytokines were reduced.Supplemented lambs also exhibited higher serum vitamin D3 concentrations and a tendency toward increased calcium levels.At the mi-crobial level,supplementation altered rumen fermentation profiles,characterized by increased acetate and butyrate concentrations and enhanced activities of microbial-associated digestive enzymes,accompanied by shifts in several fermentation-related bacterial genera.Plasma metabolomic analysis revealed alterations in pathways related to amino acid metabolism,mineral absorption,and protein digestion and absorption.In addition,offspring-directed supplementation accompanied by in maternal body condition,immune indices,and plasma metabolic pathways linked to energy metabolism.Overall,these findings indicate that early-life milk-based nutritional supplementation is associated with coordinated microbial,metabolic,and immune responses in lambs and may influence physiological status within the maternal-offspring unit,supporting further investiga-tion of early-life nutritional strategies in food bioscience.展开更多
Neuro-oxidative stress mediated by reactive oxygen and nitrogen species has been widely implicated in the pathogenesis of Parkinson's disease(PD).Polydeoxyribonucleotide(PDRN),a DNA-derived biopolymer with reporte...Neuro-oxidative stress mediated by reactive oxygen and nitrogen species has been widely implicated in the pathogenesis of Parkinson's disease(PD).Polydeoxyribonucleotide(PDRN),a DNA-derived biopolymer with reported anti-inflammatory properties,has not been fully explored in the context of PD.In this study,PDRN purified from heat-inactivated Enterococcus faecium FBL1(HEF PDRN)was structurally characterized by electrophoresis and Fourier transform infrared spectroscopy.Its cytoprotective effects were evaluated in MPTPinduced SH-SY5Y and C2C12 cells,and its in vivo effects were examined in an MPTP-induced PD mouse model using behavioral assays,histological analysis,transcriptomics,and molecular profiling.HEF PDRN treatment was associated with improved motor performance in rotarod,grip strength,and wire-hanging tests,as well as reduced immobility in the forced swim test.Histological and immunohistochemical analyses indicated attenuation of MPTP-induced muscle damage,preservation of dopaminergic neurons,and reducedα-synuclein aggregation.Transcriptomic analysis revealed attenuation of MPTP-induced suppression of neuroprotective(Park7,and Sqstm1),myogenic(Myf5,MyoG,and Myh1),and osteogenic-associated(Bmp2,Runx2,and Wnt5b)gene expression,with enrichment of Wnt/β-catenin and BMP/SMAD signaling pathways.These effects were accompanied by modulation of MAPK signaling and activation of the adenosine A2A receptor,together with changes inβ-catenin levels.Overall,HEF PDRN may represent a food fermentation-derived bioactive compound associated with antioxidant and anti-inflammatory signaling responses,along with modulation of MAPKmediated BMP/SMAD/Wnt pathways,under neuro-oxidative stress.These findings suggest its potential relevance for the development of functional food ingredients targeting neuroprotective and neuromuscularassociated responses within an acute neurotoxicity model.展开更多
文摘The safety of crop protection products and their safe use is of paramount importance and requires that crop protection products are consistently marketed and used worldwide in the required registered quality.To meet these high-quality requirements,the highest demands must be placed on the production and purification of technical-grade crop protection products.In this context,impurity profiles are of utmost importance,as unknown impurities at higher than registered concentrations may pose new unknown toxicological risks to users,consumers,and the environment.Particularly high demands are placed on the production and purification of technical grade semisynthetic derivatives of fermentatively derived natural products to meet the required regulatory specifications,to ensure that relevant impurities do not exceed maximum allowable concentrations,to ensure the safe use of such products,and to eliminate any unknown product safety risks.The challenges associated with the manufacture and purification of Afidopyropen and the product safety risks associated with various qualities of technical grade Emamectin benzoate are discussed in detail.
基金supported by the National Key Research and Development Program,China(2022YFD1302102)Agricultural Science and Technology Innovation Program,China(ASTIPIAS07)China Scholarship Council,China(202503250014).
文摘Early life is a critical period for metabolic and immune development,during which nutritional inputs may in-fluence physiological maturation beyond basic nutrient supply.This study evaluated a milk-based nutritional formulation as an early-life intervention in lambs.The study further examined associations with microbial fermentation,systemic metabolism,immune responses,growth performance,and maternal physiological re-sponses.Synthetic milk supplementation resulted in body weight gain and average daily gain in lambs,together with elevated circulating growth hormone and insulin-like growth factor-1 levels.Immune indicators,including IgA and IgM,were increased,while pro-inflammatory cytokines were reduced.Supplemented lambs also exhibited higher serum vitamin D3 concentrations and a tendency toward increased calcium levels.At the mi-crobial level,supplementation altered rumen fermentation profiles,characterized by increased acetate and butyrate concentrations and enhanced activities of microbial-associated digestive enzymes,accompanied by shifts in several fermentation-related bacterial genera.Plasma metabolomic analysis revealed alterations in pathways related to amino acid metabolism,mineral absorption,and protein digestion and absorption.In addition,offspring-directed supplementation accompanied by in maternal body condition,immune indices,and plasma metabolic pathways linked to energy metabolism.Overall,these findings indicate that early-life milk-based nutritional supplementation is associated with coordinated microbial,metabolic,and immune responses in lambs and may influence physiological status within the maternal-offspring unit,supporting further investiga-tion of early-life nutritional strategies in food bioscience.
基金supported by the National Research Foundation of the Republic of Korea(NRF)grant funded by the Republic of the Korean Government(MSIT)(RS-2023-NR077274).
文摘Neuro-oxidative stress mediated by reactive oxygen and nitrogen species has been widely implicated in the pathogenesis of Parkinson's disease(PD).Polydeoxyribonucleotide(PDRN),a DNA-derived biopolymer with reported anti-inflammatory properties,has not been fully explored in the context of PD.In this study,PDRN purified from heat-inactivated Enterococcus faecium FBL1(HEF PDRN)was structurally characterized by electrophoresis and Fourier transform infrared spectroscopy.Its cytoprotective effects were evaluated in MPTPinduced SH-SY5Y and C2C12 cells,and its in vivo effects were examined in an MPTP-induced PD mouse model using behavioral assays,histological analysis,transcriptomics,and molecular profiling.HEF PDRN treatment was associated with improved motor performance in rotarod,grip strength,and wire-hanging tests,as well as reduced immobility in the forced swim test.Histological and immunohistochemical analyses indicated attenuation of MPTP-induced muscle damage,preservation of dopaminergic neurons,and reducedα-synuclein aggregation.Transcriptomic analysis revealed attenuation of MPTP-induced suppression of neuroprotective(Park7,and Sqstm1),myogenic(Myf5,MyoG,and Myh1),and osteogenic-associated(Bmp2,Runx2,and Wnt5b)gene expression,with enrichment of Wnt/β-catenin and BMP/SMAD signaling pathways.These effects were accompanied by modulation of MAPK signaling and activation of the adenosine A2A receptor,together with changes inβ-catenin levels.Overall,HEF PDRN may represent a food fermentation-derived bioactive compound associated with antioxidant and anti-inflammatory signaling responses,along with modulation of MAPKmediated BMP/SMAD/Wnt pathways,under neuro-oxidative stress.These findings suggest its potential relevance for the development of functional food ingredients targeting neuroprotective and neuromuscularassociated responses within an acute neurotoxicity model.
